BGC0000026

BGC0000026: avilamycin A biosynthetic gene cluster from Streptomyces viridochromogenes Tue57

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Location: 1,590 - 39,999 nt. (total: 38,410 nt).
This entry is originally from NCBI GenBank AF333038.2.

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core biosynthetic genes

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Gene details

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General

Compounds

Genes

Saccharide

History

KnownClusterBlast

General information about the BGC

MIBiG accession	BGC0000026
Short description	avilamycin A biosynthetic gene cluster from Streptomyces viridochromogenes Tue57
Status	Minimal annotation: no A minimal annotation only contains information on the BGC loci and one or more linked chemical product(s) Completeness: incomplete Whether the loci encodes everything needed for the pathway producing the compound(s)
Biosynthetic class(es)	Saccharide (oligosaccharide)
Loci	NCBI GenBank: AF333038.2
Compounds	avilamycin A avilamycin C
Species	Streptomyces viridochromogenes Tue57 [taxonomy]
References	Biosynthesis of the orthosomycin antibiotic avilamycin A: deductions from the molecular analysis of the avi biosynthetic gene cluster of Streptomyces viridochromogenes Tü57 and production of new antibiotics. Weitnauer G et al., Chem Biol (2001) PMID:11410376 An ATP-binding cassette transporter and two rRNA methyltransferases are involved in resistance to avilamycin in the producer organism Streptomyces viridochromogenes Tü57. Weitnauer G et al., Antimicrob Agents Chemother (2001) PMID:11181344 Analysis of a C-methyltransferase gene (aviG1) involved in avilamycin biosynthesis in Streptomyces viridochromogenes Tü57 and complementation of a Saccharopolyspora erythraea eryBIII mutant by aviG1. Weitnauer G et al., Microbiology (Reading) (2002) PMID:11832501 Novel avilamycin derivatives with improved polarity generated by targeted gene disruption. Weitnauer G et al., Chem Biol (2004) PMID:15489167 Genes involved in formation and attachment of a two-carbon chain as a component of eurekanate, a branched-chain sugar moiety of avilamycin A. Treede I et al., Appl Environ Microbiol (2005) PMID:15640214 Genes encoding enzymes responsible for biosynthesis of L-lyxose and attachment of eurekanate during avilamycin biosynthesis. Hofmann C et al., Chem Biol (2005) PMID:16242656 The active conformation of avilamycin A is conferred by AviX12, a radical AdoMet enzyme. Boll R et al., J Biol Chem (2006) PMID:16537546 Insights into the synthesis of lipopolysaccharide and antibiotics through the structures of two retaining glycosyltransferases from family GT4. Martinez-Fleites C et al., Chem Biol (2006) PMID:17113996 Production of avilamycin A is regulated by AviC1 and AviC2, two transcriptional activators. Rebets Y et al., J Antibiot (Tokyo) (2009) PMID:19609289 Structure and function of the antibiotic resistance-mediating methyltransferase AviRb from Streptomyces viridochromogenes. Mosbacher TG et al., J Mol Biol (2005) PMID:15581897 The avilamycin resistance determinants AviRa and AviRb methylate 23S rRNA at the guanosine 2535 base and the uridine 2479 ribose. Treede I et al., Mol Microbiol (2003) PMID:12828631 Evernimicin binds exclusively to the 50S ribosomal subunit and inhibits translation in cell-free systems derived from both gram-positive and gram-negative bacteria. McNicholas PM et al., Antimicrob Agents Chemother (2000) PMID:10770739 Flambamycin, a new antibiotic from Streptomyces hygroscopicus DS 23 230. Ninet L et al., Experientia (1974) PMID:4435163

Chemical products information

avilamycin A

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C₆₁H₈₈Cl₂O₃₂

Chemical database entries
PubCHEM
ChemSpider

avilamycin C

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C₆₁H₉₀Cl₂O₃₂

Chemical database entries
PubCHEM
ChemSpider

List of genes involved in compound(s) production

Identifiers	Position	Product	Functions	Evidence	Extra
AAK83161.1 aviX1	1590 - 2678 (+)	putative GTP binding protein			copy AA seq copy Nt seq
AAK83162.1 aviX2	2870 - 3151 (+)	AviX2			copy AA seq copy Nt seq
AAK83163.1 aviX3	3148 - 3402 (+)	AviX3			copy AA seq copy Nt seq
AAK83164.1 aviX4	3624 - 4310 (-)	AviX4			copy AA seq copy Nt seq
AAK83165.1 aviX5	4307 - 4852 (-)	AviX5			copy AA seq copy Nt seq
AAG32066.1 aviRb	4931 - 5794 (+)	rRNA methyltransferase	Resistance/immunity	Activity assay	copy AA seq copy Nt seq
AAK83166.1 aviX6	5809 - 6159 (+)	AviX6			copy AA seq copy Nt seq
AAK83167.1 aviX7	6159 - 6305 (+)	AviX7			copy AA seq copy Nt seq
AAK83168.1 aviX8	6489 - 6830 (+)	AviX8			copy AA seq copy Nt seq
AAG32067.2 aviRa	6930 - 7682 (-)	rRNA methyltransferase AviRa	Resistance/immunity	Activity assay	copy AA seq copy Nt seq
AAK83169.1 aviQ1	7935 - 8903 (+)	putative UDP-glucose 4-epimerase			copy AA seq copy Nt seq
AAK83170.1 aviGT2	9018 - 9740 (-)	putative glycosyltransferase			copy AA seq copy Nt seq
AAK83171.1 aviX9	9960 - 11027 (+)	AviX9			copy AA seq copy Nt seq
AAK83172.1 aviC1	11060 - 11680 (-)	putative two-component regulator	Regulation	Heterologous expression Knock-out	copy AA seq copy Nt seq
AAK83173.1 aviC2	11951 - 12529 (-)	putative response regulator	Regulation	Heterologous expression Knock-out	copy AA seq copy Nt seq
AAK83174.1 aviX10	12685 - 13095 (+)	AviX10			copy AA seq copy Nt seq
AAK83175.1 aviX11	13092 - 13787 (+)	AviX11			copy AA seq copy Nt seq
AAK83176.1 aviG1	13784 - 15007 (+)	methyltransferase	Precursor biosynthesis	Heterologous expression Knock-out	copy AA seq copy Nt seq
AAK83177.1 aviJ	15012 - 16442 (+)	putative transporter			copy AA seq copy Nt seq
AAK83178.1 aviN	16593 - 17624 (+)	AviN			copy AA seq copy Nt seq
AAK83194.1 aviM	17621 - 21502 (+)	polyketide synthase			copy AA seq copy Nt seq
AAK83195.1 aviD	21692 - 22759 (+)	putative dTDP glucose synthetase			copy AA seq copy Nt seq
AAK83196.1 aviE1	22810 - 23877 (+)	putative dTDP-glucose 4,6-dehydratase			copy AA seq copy Nt seq
AAK83179.1 aviQ2	23925 - 24953 (+)	putative NDP-glucose 4-epimerase			copy AA seq copy Nt seq
AAK83180.1 aviG5	25011 - 25610 (+)	putative methyltransferase	Tailoring (Methylation)	Knock-out	copy AA seq copy Nt seq
AAK83181.1 aviO1	25761 - 26432 (+)	putative oxygenase			copy AA seq copy Nt seq
AAK83182.1 aviGT1	26490 - 27782 (+)	putative glycosyltransferase			copy AA seq copy Nt seq
AAK83183.1 aviE2	27779 - 28792 (+)	UDP-GlcA decarboxylase	Precursor biosynthesis	Activity assay	copy AA seq copy Nt seq
AAK83184.1 aviG2	28789 - 29511 (+)	putative methyltransferase	Tailoring (Methylation)	Knock-out	copy AA seq copy Nt seq
AAK83185.1 aviZ1	29508 - 30500 (+)	putative oxidoreductase			copy AA seq copy Nt seq
AAK83186.1 aviG6	30514 - 31233 (+)	putative methyltransferase	Tailoring (Methylation)	Knock-out	copy AA seq copy Nt seq
AAK83187.1 aviO3	31230 - 32174 (+)	putative oxygenase			copy AA seq copy Nt seq
AAK83188.1 aviG3	32171 - 33004 (+)	AviG3			copy AA seq copy Nt seq
AAK83189.1 aviX12	33001 - 34191 (+)	C-epimerase	Activation / processing	Knock-out	copy AA seq copy Nt seq
AAG32068.1 aviABC1	34188 - 35159 (+)	putative ATP binding protein			copy AA seq copy Nt seq
AAG32069.1 aviABC2	35156 - 35953 (+)	putative transmembrane protein			copy AA seq copy Nt seq
AAK83190.1 aviB1	35950 - 36954 (+)	putative pyruvate dehydrogenase	Precursor biosynthesis	Knock-out	copy AA seq copy Nt seq
AAK83191.1 aviB2	36954 - 37916 (+)	putative pyruvate dehydrogenase			copy AA seq copy Nt seq
AAK83192.1 aviGT3	37955 - 38971 (+)	putative glycosyltransferase			copy AA seq copy Nt seq
AAK83193.1 aviGT4	38971 - 39999 (+)	putative glycosyltransferase			copy AA seq copy Nt seq

Saccharide-specific information

Subclass	oligosaccharide
Glycosyltransferases	aviGT1 Evidence: Sequence-based prediction
	aviGT2 Evidence: Sequence-based prediction
	aviGT3 Evidence: Sequence-based prediction
	aviGT4 Specificity: eurekanate Evidence: Knock-out construct

Annotation changelog

MIBiG version	Submitter	Notes
1.0	Hidden contributor (ID: LPHAMZXQ3BT6INJWOLKUYGWU, no GDPR consent given).	Submitted
2.0	Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).	Migrated from v1.4
3.0	Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).	Cleaned up synonym list
3.1	Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).	Update chemical activity to schema version 2.11

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