LOCUS       BGC0000037              7821 bp    DNA     linear   PLN 27-FEB-2015
DEFINITION  TPA_asm: Aspergillus nidulans FGSC A4 chromosome I.
ACCESSION   BGC0000037
VERSION     BGC0000037
KEYWORDS    .
SOURCE      Aspergillus nidulans FGSC A4
  ORGANISM  Aspergillus nidulans FGSC A4
            Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina;
            Eurotiomycetes; Eurotiomycetidae; Eurotiales; Aspergillaceae;
            Aspergillus; Aspergillus subgen. Nidulantes.
COMMENT     Aspergillus nidulans FGSC A4 CADRE,
            updated by Eurofungbase with contributions from
            CADRE (http://www.cadre-genomes.org.uk),
            AspGD (http://www.aspergillusgenome.org) and
            Ensembl Genomes (http://www.ensemblgenomes.org).
            ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-07 14:58:49
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     mRNA            complement(join(1..959,1018..5438,5600..7158,7207..7821))
                     /locus_tag="ANIA_06448"
                     /note="transcript_id=CADANIAT00006526"
                     /old_locus_tag="AN6448.4"
     gene            complement(1..7821)
                     /locus_tag="ANIA_06448"
                     /old_locus_tag="AN6448.4"
     protocluster    1..7821
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[0:7821]"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1..7821
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
     cand_cluster    1..7821
                     /SMILES="CC(=O)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(PKS_AT and (PKS_KS or ene_KS or
                     mod_KS or hyb_KS or itr_KS or tra_KS))"
                     /kind="single"
                     /product="T1PKS"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..7821
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000037"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..7821
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T1PKS"
                     /region_number="1"
                     /rules="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS))"
                     /subregion_numbers="1"
                     /tool="antismash"
     CDS             complement(join(1..959,1018..5438,5600..7158,7207..7821))
                     /NRPS_PKS="Domain: SAT (91-242). E-value: 7e-10. Score:
                     30.7. Matches aSDomain: nrpspksdomains_ANIA_06448_SAT.1"
                     /NRPS_PKS="Domain: PKS_KS(Iterative-KS) (382-727). E-value:
                     5.9e-130. Score: 425.8. Matches aSDomain:
                     nrpspksdomains_ANIA_06448_PKS_KS.1"
                     /NRPS_PKS="Domain: PKS_AT (849-1137). E-value: 7.1e-59.
                     Score: 191.4. Matches aSDomain:
                     nrpspksdomains_ANIA_06448_PKS_AT.1"
                     /NRPS_PKS="Domain: PKS_DH2 (1248-1446). E-value: 4.8e-08.
                     Score: 24.5. Matches aSDomain:
                     nrpspksdomains_ANIA_06448_PKS_DH2.1"
                     /NRPS_PKS="Domain: PP-binding (1580-1646). E-value: 8e-10.
                     Score: 31.0. Matches aSDomain:
                     nrpspksdomains_ANIA_06448_PP-binding.1"
                     /NRPS_PKS="Domain: PP-binding (1692-1757). E-value:
                     2.6e-12. Score: 39.0. Matches aSDomain:
                     nrpspksdomains_ANIA_06448_PP-binding.2"
                     /NRPS_PKS="Domain: cMT (1955-2128). E-value: 6.5e-49.
                     Score: 157.8. Matches aSDomain:
                     nrpspksdomains_ANIA_06448_cMT.1"
                     /codon_start=1
                     /db_xref="EnsemblGenomes-Gn:CADANIAG00006526"
                     /db_xref="EnsemblGenomes-Tr:CADANIAT00006526"
                     /db_xref="GOA:Q5AZ32"
                     /db_xref="InterPro:IPR001227"
                     /db_xref="InterPro:IPR001375"
                     /db_xref="InterPro:IPR006162"
                     /db_xref="InterPro:IPR009081"
                     /db_xref="InterPro:IPR013094"
                     /db_xref="InterPro:IPR013217"
                     /db_xref="InterPro:IPR014030"
                     /db_xref="InterPro:IPR014031"
                     /db_xref="InterPro:IPR014043"
                     /db_xref="InterPro:IPR016035"
                     /db_xref="InterPro:IPR016036"
                     /db_xref="InterPro:IPR016038"
                     /db_xref="InterPro:IPR016039"
                     /db_xref="InterPro:IPR029058"
                     /db_xref="InterPro:IPR029063"
                     /db_xref="UniProtKB/TrEMBL:Q5AZ32"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     itr_KS"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_AT"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S9"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022:Beta-ketoacyl synthase (Score: 200.1; E-value:
                     1.1e-60)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ANIA_06448"
                     /note="transcript_id=CADANIAT00006526"
                     /old_locus_tag="AN6448.4"
                     /product="polyketide synthase, putative (JCVI)"
                     /protein_id="CBF69451.1"
                     /sec_met_domain="PP-binding (E-value: 4.7e-14, bitscore:
                     39.0, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="PKS_AT (E-value: 1.3e-60, bitscore: 191.4,
                     seeds: 1682, tool: rule-based-clusters)"
                     /sec_met_domain="itr_KS (E-value: 1.2e-133, bitscore:
                     432.1, seeds: 96, tool: rule-based-clusters)"
                     /sec_met_domain="Peptidase_S9 (E-value: 1e-11, bitscore:
                     30.9, seeds: 64, tool: rule-based-clusters)"
                     /transl_table=1
                     /translation="MAPAPSALVFGSQTTLPSVEAASRLRAALLLDPRLYRMRTSIESL
                     PEIWPALAISDPALERVAGPAEKSLRQLCRWLSHSEFPDATEISELPATFVTPFTVILH
                     TVLYMHYTDENGSRGHADVLRAVRNNGGVQGFCTGFLTAVSVATSPDLEALSRQASVAL
                     RLAVAIGAYIDLDLLDSDVSSVAVRSRAGKKGLEETLAQFPGAYISVITDELNATVTAP
                     RASLDALSQSLASNGLSAKRFDLRGRFHHPAHQKALEGLYNLVASNPVFQFSHSELLAP
                     VYSNIDGQLLSSDSIIDTLLQSILVQRCDWYASISTALHKRSNGEKTSVVQFSLVECIP
                     PSVLRQARLSVQRITDVPVQNSPATVPAPLNAVRARIQTSEPIAIIGMGCKFPGADTLD
                     EYWQLLAQGTSMCRTMPEERFKTSSLRRSPGEKLKFWGNFVNDVDAFDHRFFKKSSREA
                     ASMDPQQRLVLQVAYQTLESAEYSGLSGIKASRDVGCYLGLCASDYTDNVASHPPNAFS
                     SLGTLRAFLSGKISHFFGWTGPSITYDTACSSSAVAIQAACRALQTGECSMALAGGVSL
                     YTSPNFYQNLSAASFLSPTGPTKPFDAKGDGYCRGEGVGLVFLKPLSSALADHDNIMGV
                     IAAAAVNQNQNSTAITVPHSESQIELYRKVVSEAGLHPHDVSFVEAHGTGTPVGDPIEF
                     TSIRTVFGGSNRANPLAIASVKGNIGHTEGAAACINNYGAAGSNAAMIVTEAPTGARSE
                     KQGTLPKCPIYVSANTVSSLKEYCKELLRSLRGRSPDCLASLAFQLANSQNRGFPHALI
                     TSVTSKAELEDQLSAVVENRNNSLHTVAPTERPVVLAFGGQVARSVGLSRQVYDSSAVL
                     RTHLSNCDDILTSAGLNSIFPAIFRKEPIADVVLLHSALFSAQYACAMTWIEAGVIPAA
                     LIGHSFGQLTALSVGRVLSLKDGLGLITERAKLMRDAWGPEHGSMVSVQADVQTVARLM
                     KAAETKDPKDALEIACFNGPTSHVVVGSADAADRLEAALTQESIRYKRLAVSHGFHSKF
                     TEPLLLGLEQCAERLTFRTPKYAIETCSSGSSWSEFNASMIVQHTRTPVYYTEALARIE
                     AKLGACTWLEVGTGGSVAGMIRGALNVPSDHLIQAVNLAGETGTAALADATVNLWKSSH
                     KLQFWAFHRSERECYQPLELPPYQFEKTRHWLDWKDTMTEQATVTQSTREETSVEEFLT
                     FVKYKDSTKQQAEFRISTEHEKYSFFVKGHAVLAEPLCPAPLYIDLACKAGQMVYSDTS
                     ETLIIPSVEDLEIQAPLGVGDRVIILRLQQSPFLKTAWTFCFCSRPVMGNSAEEQLHAS
                     GTVVLRENDTKTAAEFSRFGRLVSSKRVQEMKSDPDCHILQGPVVYQLFSRVVSYADYY
                     KGVQSVYASGAEVTGRIRLPPTVKDADTRRPLLVDNFIQTAGIHVNCLTDVGAKEVYVC
                     TKVDRVQSAAAFTEDLANVDASWIVHSSYHPTSEKEVVNDIFVFNAATGELAMFILGAH
                     FTRVQISSLGRVLSRANTADAAPIKVAVPVQSPALRAQPKRVLLPPLTKRSITRPTLEI
                     SEKLKKTLSRVVEVPVADIHDGGILADLGVDSLLGTEVLTEINQVFNVSIPADEFALLT
                     DVASISKCLASYLGVHDSGSQPEDLADADSVESDSDMPTGAVTSGITTPDDAVSRLADL
                     LAENLEYDGTIEASNNLADLGLDSILSIELANDIKKIFNCDVDMSQLNMESTFADLIAL
                     VPALNIEQSLSSVPASLTTQGSDFEMAQHAFEQIRFDYDIYTKETGFYDFWKRVYPAQS
                     RLVLAYTVEAFAQLGCDLALMHPGDRLPKIGYLPAHEKLVQQLYNILRDGMLVATSDSG
                     FVRSDKPVDPTSSTRLLEEINTIAPQHASEHSLLHITGSKLADCLTGTADPLNLLFRSK
                     ANRDLLAEVYLNGPMYAAISRLLCSFLGNAFSDRQSSGTFQILELGGGTGGTTGHVLDY
                     LVRSGIPFTYTFSDVSGSFVAAARKKFAGRPYMEYQVIDIEKEPADSLTGKFHAVISTN
                     CIHATTNLEISTGNIHRMLRPDGFVALVEFTRNMYWFDLVFGLLEGWWLFEDGRQHVIA
                     SESFWNTSMRKAGFQHVSWTDGDSFEARTLRIIAGFRAPAVNEIYTPRLDSRDTETAVE
                     SVMYKQADGIPLFADIYYPPSVSNEPRPIALMIHGGGHIMLSRKDIRPKQTAHLHTLGF
                     LPVSIDYRLCPETTLTEGPMRDVSDAMAWARSTLCSLPLLCPGLTLDPSRVVVVGWSTG
                     GHLAMTTAFTSIERGLSPPDAILAFYCPTDYEDRVWTQPNYPENTDVDGLSLMKYNLLE
                     GVQDRPITAYNIPLTSRSNSKAGGTPAGGWMAPDDPRSRIVLHMNWKGQCLPVLLRGLP
                     PSNSLSPGDAEKLISQPQPEIEEIQRVSPYAQIRRGVYRTPTFVIHGTDDDLIPYEQSV
                     RTVQALKDMGVRAEVSVPQGKAHLFDMFKDADGSSWEVVKRGYDFLKEEVSGK"
     CDS_motif       complement(670..717)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ANIA_06448_0006"
                     /evalue="1.90E-03"
                     /label="NRPS-te1"
                     /locus_tag="ANIA_06448"
                     /protein_end="2295"
                     /protein_start="2279"
                     /score="10.8"
                     /tool="antismash"
                     /translation="VVVGWSTGGHLAMTTA"
     aSDomain        complement(1229..1747)
                     /aSDomain="MT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ANIA_06448_cMT.1"
                     /domain_subtype="cMT"
                     /evalue="6.50E-49"
                     /label="ANIA_06448_cMT.1"
                     /locus_tag="ANIA_06448"
                     /protein_end="2128"
                     /protein_start="1955"
                     /score="157.8"
                     /tool="antismash"
                     /translation="LCSFLGNAFSDRQSSGTFQILELGGGTGGTTGHVLDYLVRSGIPF
                     TYTFSDVSGSFVAAARKKFAGRPYMEYQVIDIEKEPADSLTGKFHAVISTNCIHATTNL
                     EISTGNIHRMLRPDGFVALVEFTRNMYWFDLVFGLLEGWWLFEDGRQHVIASESFWNTS
                     MRKAGFQHVS"
     aSDomain        complement(2342..2536)
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ANIA_06448_PP-binding.2"
                     /evalue="2.60E-12"
                     /label="ANIA_06448_PP-binding.2"
                     /locus_tag="ANIA_06448"
                     /protein_end="1757"
                     /protein_start="1692"
                     /score="39.0"
                     /tool="antismash"
                     /translation="RLADLLAENLEYDGTIEASNNLADLGLDSILSIELANDIKKIFNC
                     DVDMSQLNMESTFADLIALV"
     aSDomain        complement(2675..2872)
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ANIA_06448_PP-binding.1"
                     /evalue="8.00E-10"
                     /label="ANIA_06448_PP-binding.1"
                     /locus_tag="ANIA_06448"
                     /protein_end="1646"
                     /protein_start="1580"
                     /score="31.0"
                     /tool="antismash"
                     /translation="EKLKKTLSRVVEVPVADIHDGGILADLGVDSLLGTEVLTEINQVF
                     NVSIPADEFALLTDVASISKC"
     aSModule        2675..6627
                     /complete
                     /domains="nrpspksdomains_ANIA_06448_PKS_KS.1"
                     /domains="nrpspksdomains_ANIA_06448_PKS_AT.1"
                     /domains="nrpspksdomains_ANIA_06448_PKS_DH2.1"
                     /domains="nrpspksdomains_ANIA_06448_PP-binding.1"
                     /iterative
                     /locus_tags="ANIA_06448"
                     /monomer_pairings="mal -> mal"
                     /tool="antismash"
                     /type="pks"
     aSDomain        complement(3275..3868)
                     /aSDomain="PKS_DH2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ANIA_06448_PKS_DH2.1"
                     /evalue="4.80E-08"
                     /label="ANIA_06448_PKS_DH2.1"
                     /locus_tag="ANIA_06448"
                     /protein_end="1446"
                     /protein_start="1248"
                     /score="24.5"
                     /tool="antismash"
                     /translation="SFFVKGHAVLAEPLCPAPLYIDLACKAGQMVYSDTSETLIIPSVE
                     DLEIQAPLGVGDRVIILRLQQSPFLKTAWTFCFCSRPVMGNSAEEQLHASGTVVLREND
                     TKTAAEFSRFGRLVSSKRVQEMKSDPDCHILQGPVVYQLFSRVVSYADYYKGVQSVYAS
                     GAEVTGRIRLPPTVKDADTRRPLLVDNFIQTAGIH"
     aSDomain        complement(4202..5065)
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ANIA_06448_PKS_AT.1"
                     /evalue="7.10E-59"
                     /label="ANIA_06448_PKS_AT.1"
                     /locus_tag="ANIA_06448"
                     /protein_end="1137"
                     /protein_start="849"
                     /score="191.4"
                     /specificity="consensus: mal"
                     /specificity="PKS signature: Malonyl-CoA"
                     /specificity="Minowa: mal"
                     /tool="antismash"
                     /translation="FGGQVARSVGLSRQVYDSSAVLRTHLSNCDDILTSAGLNSIFPAI
                     FRKEPIADVVLLHSALFSAQYACAMTWIEAGVIPAALIGHSFGQLTALSVGRVLSLKDG
                     LGLITERAKLMRDAWGPEHGSMVSVQADVQTVARLMKAAETKDPKDALEIACFNGPTSH
                     VVVGSADAADRLEAALTQESIRYKRLAVSHGFHSKFTEPLLLGLEQCAERLTFRTPKYA
                     IETCSSGSSWSEFNASMIVQHTRTPVYYTEALARIEAKLGACTWLEVGTGGSVAGMIRG
                     ALNVPSD"
     CDS_motif       complement(4379..4411)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ANIA_06448_0005"
                     /evalue="6.80E+01"
                     /label="PKSI-KS_m3"
                     /locus_tag="ANIA_06448"
                     /protein_end="1078"
                     /protein_start="1067"
                     /score="-2.5"
                     /tool="antismash"
                     /translation="PKYAIETCSSG"
     CDS_motif       complement(4721..4840)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ANIA_06448_0004"
                     /evalue="1.80E-14"
                     /label="PKSI-AT-M_m3"
                     /locus_tag="ANIA_06448"
                     /protein_end="964"
                     /protein_start="924"
                     /score="45.7"
                     /tool="antismash"
                     /translation="GVIPAALIGHSFGQLTALSVGRVLSLKDGLGLITERAKLM"
     aSDomain        complement(join(5432..5438,5600..6627))
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ANIA_06448_PKS_KS.1"
                     /domain_subtype="Iterative-KS"
                     /evalue="5.90E-130"
                     /label="ANIA_06448_PKS_KS.1"
                     /locus_tag="ANIA_06448"
                     /protein_end="727"
                     /protein_start="382"
                     /score="425.8"
                     /tool="antismash"
                     /translation="IAIIGMGCKFPGADTLDEYWQLLAQGTSMCRTMPEERFKTSSLRR
                     SPGEKLKFWGNFVNDVDAFDHRFFKKSSREAASMDPQQRLVLQVAYQTLESAEYSGLSG
                     IKASRDVGCYLGLCASDYTDNVASHPPNAFSSLGTLRAFLSGKISHFFGWTGPSITYDT
                     ACSSSAVAIQAACRALQTGECSMALAGGVSLYTSPNFYQNLSAASFLSPTGPTKPFDAK
                     GDGYCRGEGVGLVFLKPLSSALADHDNIMGVIAAAAVNQNQNSTAITVPHSESQIELYR
                     KVVSEAGLHPHDVSFVEAHGTGTPVGDPIEFTSIRTVFGGSNRANPLAIASVKGNIGHT
                     EGAAA"
     CDS_motif       complement(5695..5742)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ANIA_06448_0003"
                     /evalue="3.30E-04"
                     /label="PKSI-KS_m5"
                     /locus_tag="ANIA_06448"
                     /protein_end="693"
                     /protein_start="677"
                     /score="12.8"
                     /tool="antismash"
                     /translation="FVEAHGTGTPVGDPIE"
     CDS_motif       complement(5935..5973)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ANIA_06448_0002"
                     /evalue="3.90E-04"
                     /label="PKSI-KS_m4"
                     /locus_tag="ANIA_06448"
                     /protein_end="613"
                     /protein_start="600"
                     /score="13.5"
                     /tool="antismash"
                     /translation="FDAKGDGYCRGEG"
     CDS_motif       complement(6115..6162)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ANIA_06448_0001"
                     /evalue="3.80E-05"
                     /label="PKSI-KS_m3"
                     /locus_tag="ANIA_06448"
                     /protein_end="553"
                     /protein_start="537"
                     /score="15.9"
                     /tool="antismash"
                     /translation="GPSITYDTACSSSAVA"
     aSDomain        complement(join(7048..7158,7207..7548))
                     /aSDomain="SAT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ANIA_06448_SAT.1"
                     /evalue="7.00E-10"
                     /label="ANIA_06448_SAT.1"
                     /locus_tag="ANIA_06448"
                     /protein_end="242"
                     /protein_start="91"
                     /score="30.7"
                     /tool="antismash"
                     /translation="PATFVTPFTVILHTVLYMHYTDENGSRGHADVLRAVRNNGGVQGF
                     CTGFLTAVSVATSPDLEALSRQASVALRLAVAIGAYIDLDLLDSDVSSVAVRSRAGKKG
                     LEETLAQFPGAYISVITDELNATVTAPRASLDALSQSLASNGLSAKR"
ORIGIN
        1 ttacttccca gacacctcct ccttcaaaaa atcgtaccct ctctttacaa cttcccacga
       61 actcccgtct gcgtctttga acatatcaaa cagatgcgcc tttccctgtg gcacactgac
      121 ttcagcacga acgcccatgt ccttcaaagc ctgcacagtc cgtacactct gctcataggg
      181 gataagatca tcatctgtgc catgaatgac aaatgttggg gtgcgataaa ctccccttcg
      241 gatctgcgcg tagggactaa cacgctggat ctcctcaatc tctggttgtg gttgagaaat
      301 caacttctca gcatcgcctg gagaaaggga attgctagga ggcaatccac gcaacaaaac
      361 gggaagacac tggcccttcc aattcatatg caggacaatc cttgatcggg ggtcatccgg
      421 tgccatccat cccccggctg gagtcccacc tgccttagaa ttagacctag atgtaagggg
      481 gatgttgtaa gctgttatcg gacgatcctg tacaccctcg agcaggttat acttcatcag
      541 ggatagacca tcaacatctg tattctctgg ataatttggc tgcgtccaaa cacggtcctc
      601 gtagtcggtc gggcagtaaa aagccagaat tgcgtcggga ggagataggc cccgttcaat
      661 ggatgtgaat gctgttgtca tggcaagatg cccgcctgtc gaccagccga caacgacaac
      721 gcgcgaggga tcaagggtca agccaggaca cagtaatggc aacgaacaaa gggtactccg
      781 cgcccaggcc atggcgtcgc taacatccct cattggccct tctgtcaacg tagtctcggg
      841 gcagagacgg tagtcgatag agaccgggag gaagcctagc gtgtgcaggt gcgccgtttg
      901 ctttggacga atgtccttgc gagagagcat aatgtggccg cctccgtgga tcatcagggc
      961 tgcagggttc agagtcaggt tcacagatgt gcataaaggg atggccatag atctcaccta
     1021 tcggacgcgg ttcattagag acagagggag gatagtatat gtccgcaaat agggggattc
     1081 cgtctgcctg cttgtacatc acactctcaa ccgccgtttc tgtatccctg ctatcgagtc
     1141 ttggtgtata gatctcgttg acggccggcg ctctgaaacc ggcgatgatc ctcaatgtac
     1201 gtgcctcaaa actatctcca tccgtccacg aaacatgctg gaacccagcc ttgcgcatgc
     1261 ttgtgttcca gaaggattct gatgcgatga catgctgacg cccatcctca aacaaccacc
     1321 aaccctcaag cagtccaaag acgagatcaa accaatacat attgcgtgta aactcgacaa
     1381 gtgccacaaa tccatccggg cggagcattc tgtggatgtt tcctgttgag atctcgagat
     1441 tagtcgtggc atggatgcaa ttagttgata tgactgcatg gaattttccg gttagcgagt
     1501 cggccggctc cttttcaatg tcgatgacct gatactccat atagggccgg ccagcgaact
     1561 tttttctagc ggccgcaacg aaggaaccag acacgtcgct gaatgtgtaa gtgaatggta
     1621 taccagagcg aacaaggtag tcgaggacat ggcctgttgt gcctcccgtt ccccctccta
     1681 gctccaagat ctggaatgta ccagaagact gtctatctga gaaggcgttt cctagaaagc
     1741 tgcaaaggag tcgggagata gcagcgtaca taggtccatt caggtacacc tccgccagca
     1801 agtccctgtt tgcctttgac cggaagagca ggttcagagg atcagcggtc ccagtcaagc
     1861 aatcggccaa cttagagcct gtaatatgaa gtagcgaatg ttcagaggcg tgctgcggtg
     1921 ctatcgtatt gatctcttcc agcaatcttg tcgacgatgt gggatcgaca ggcttgtctg
     1981 agcgaacaaa accagagtcg ctggtcgcaa ctaacatccc atctctcagg atgttgtata
     2041 actgctgcac aagcttctcg tgagcgggaa gatacccgat cttgggtagc cggtcgcctg
     2101 gatgcatcag ggcgagatcg catccaagct gagcaaatgc ctcgacagta taggcaagca
     2161 cgagcctgga ttgggcagga tagaccctct tccaaaagtc gtagaaacca gtctccttag
     2221 tatagatatc gtagtcgaat cgtatttgct caaacgcgtg ctgcgccatt tcgaagtcac
     2281 tgccctgcgt tgttagcgac gccggaacag acgacaggct ttgctctata ttcagggccg
     2341 gtacaagggc aattagatct gcaaaggtcg attccatgtt cagctgcgac atgtcgacgt
     2401 cgcagttaaa gatcttttta atgtcgtttg ccagctcaat gctcagtatc gaatccaggc
     2461 caagatccgc caggttgttc gatgcctcaa tagtcccgtc gtactccaaa ttttcagcca
     2521 gcaaatctgc gagcctgctt accgcgtcat ctggagtggt aatgccggac gtgacagcgc
     2581 ctgttggcat gtctgaatca gactcaacag agtctgcatc ggccaggtcc tcaggctgag
     2641 agccactgtc atgcacgccg aggtacgagg ccaagcattt cgaaattgag gccacatctg
     2701 tcaagagcgc aaattcatca gcagggatag agacattgaa tacctggttg atctcagtca
     2761 agacttctgt tcctagcagg gagtctacgc ccaggtctgc gaggattccg ccgtcgtgga
     2821 tgtcggcgac cgggacctcg acaacgcgac tcagtgtttt cttgagcttt tcagatatct
     2881 ctaaagtcgg tcttgttatc gaacgcttgg taagaggtgg cagcaagacc ctctttggtt
     2941 gtgctctcag ggcaggtgac tggaccggta cggccacctt gatcggcgca gcatctgctg
     3001 tattggcacg agagaggacc ctcccaagcg acgaaatctg gaccctggta aagtgtgctc
     3061 caagaatgaa cattgccagt tctcctgtgg ctgcattaaa gacaaatata tcattcacaa
     3121 cctccttctc actcgtcgga tgataagaag agtgcacaat ccaagaggca tcgacgttgg
     3181 ccaggtcctc ggtaaaggcc gccgcagact gtacgcggtc gaccttggtg cagacgtaga
     3241 cttccttagc accgacgtcg gttaggcagt taacatgaat gccagcggtc tggatgaaat
     3301 tgtcgacgag taatggccgc cgtgtgtcgg catccttaac ggttgggggc agcctgatcc
     3361 tgcccgtcac ttctgctcca gaagcataca cgctctgcac gcccttgtag tagtctgcat
     3421 agctgacgac tctgctgaag agctggtaca ccactggtcc ttgcaggata tgacagtctg
     3481 ggtcagattt catttcctga acacgcttgc tgcttaccag gcggccaaag cgggagaact
     3541 cggccgctgt tttagtgtcg ttttcgcgta ggacgactgt ccctgaggca tggagctgtt
     3601 cctcagctga gttgcccatg acaggtctac tgcagaaaca aaacgtccat gccgtcttta
     3661 agaagggtga ctgttgcagc cggaggatga tgacacggtc gcctacaccc agcggagcct
     3721 ggatctctag atcctcgaca ctgggtataa tgagagtctc actggtgtca gaatacacca
     3781 tctgaccagc tttgcaggca agatcaatgt ataaaggtgc agggcacaag ggctctgcga
     3841 gcacagcgtg gcctttgaca aagaaagagt acttttcgtg ctccgtgctt attcggaatt
     3901 cagcctgttg ctttgtacta tctttgtact tgacgaacgt caagaactcc tcaacagagg
     3961 tctcctccct cgttgattgg gtgactgtag cttgctcggt cattgtgtct ttccagtcaa
     4021 gccagtgtct cgtcttctcg aattggtagg ggggaagctc aagcggttgg tagcactctc
     4081 tctcgctccg gtggaaagcc cagaactgca atttatgact ggacttccag agattgaccg
     4141 tcgcatccgc cagggctgcg gtaccagtct cgcctgcgag attaacagct tggatcagat
     4201 ggtctgaggg gacattgagt gcaccacgaa tcattccagc cacggaacct ccggtaccca
     4261 cctcgagcca tgtgcatgcg cccagcttgg cctcaattcg agcgagggct tctgtgtagt
     4321 agacaggggt ccgcgtgtgt tgcacgatca tggatgcgtt gaactccgac caagatgagc
     4381 cactcgagca cgtctcgatg gcgtattttg gcgtgcggaa ggttagtctc tcagcgcact
     4441 gttccagccc aagcagtagc ggctcagtga atttggaatg gaagccatgc gagacggcga
     4501 gccgtttata cctgatagac tcctgagtaa gagcggcctc caggcgatca gcggcatctg
     4561 cactgccgac aacaacgtgg ctggtcggcc cattaaaaca ggcaatttcg agcgcatctt
     4621 ttggatcctt ggtctcagct gctttcatca ggcgagcgac ggtctggaca tctgcctgaa
     4681 ccgaaaccat cgagccatgc tctgggcccc aggcgtcccg cataagcttt gcgcgttctg
     4741 tgatcaaacc gagtccgtcc tttagagaaa ggaccctgcc gactgataga gcagtcaact
     4801 ggccaaaact gtggccgatt agggcagctg ggataacccc ggcctctatc caggtcatag
     4861 cgcaggcgta ctgggccgaa aacaacgcag agtgtagcag aacaacatca gcgattggct
     4921 ccttccgaaa tatagcagga aaaatactat ttaaaccggc tgaggtgagg atatcatcgc
     4981 aattactcag atgtgttcgg aggaccgcag aagagtcata aacctggcga ctgaggccga
     5041 ctgacctggc cacttgacca ccaaatgcga gaacaaccgg acgttctgtt ggagcaaccg
     5101 tatgcaatga attattcctg ttctcaacaa ctgctgacag ctggtcctcc agttcagctt
     5161 tcgaagtgac cgaagtaatc agtgcatgtg gaaagccgcg gttctgggag ttggcaagct
     5221 ggaaagcgag actagcaagg cagtctggag aacggccacg caaagatcgg agcagctctt
     5281 tgcagtactc cttcagactt gaaacagtat tggcagaaac gtaaatggga cactttggta
     5341 gggtcccctg cttctctgat cttgcaccgg ttggggcctc agtcacgatc atggcagcat
     5401 tgctcccagc tgcaccatag ttattgatgc aagctgcgct ttaaagttcg cagaccaggg
     5461 ctgcgttgta tgtgggatta tcaccttatc ttgctccaaa ggcgcaatct tggggttcag
     5521 tgttgtatgg ttggcctgca ttgggatcaa tccacgctgc agcatcaata ccgtcttgat
     5581 aagtgatgcc acacccgacg cgccttctgt gtgcccaatg tttcccttga ccgatgcaat
     5641 ggcgagaggg ttggctcgat tgctaccgcc gaatacggtt cgaatactgg tgaattcgat
     5701 aggatcaccc acaggcgtgc cggtcccatg ggcctcgaca aacgagacgt catgtggatg
     5761 caagccagcc tcagagacaa cctttctgta aagttcgatc tgtgactcag aatgcggcac
     5821 cgtgatggcg gtcgaatttt gattctggtt gactgcggcc gcagcaatca cccccatgat
     5881 gttatcgtga tcggccaggg ctgaagatag gggtttcaaa aagaccaacc ccaccccttc
     5941 tccacgacag taaccatctc cctttgcatc aaatggcttc gttgggccag taggactcag
     6001 gaaagaagcg gctgagaggt tctggtagaa gttagggctc gtgtacagac tgactccccc
     6061 cgctagcgcc atcgagcatt cgcccgtctg caaagcccgg caggcagctt gaattgctac
     6121 agctgaggaa gagcatgcag tgtcatatgt aattgacggt cctgtccagc cgaagaaatg
     6181 gctgattttt ccggatagaa atgccctcag tgttccaagg ctggaaaatg cattgggtgg
     6241 gtgtgaggcc acgttatcgg tgtagtctga ggcacataac cccagataac acccgacatc
     6301 tctgctggcc ttgatcccag agagaccaga atactccgcc gactctaatg tctgataagc
     6361 aacctgcagc acaagccgct gttgaggatc catgctcgcc gcctcacgag acgacttctt
     6421 gaagaaccga tggtcaaagg catccacgtc attgacaaag ttaccccaga acttgagttt
     6481 ctcgcccggc gatcttcgta aactagatgt cttgaatcgt tcctcgggca tggtacggca
     6541 catggatgtc ccttgagcta gcagctgcca gtactcgtcc agggtatccg cccccggaaa
     6601 cttgcagccc atgccgatga ttgcgatggg ctctgaagtc tgtattcttg ctctcacagc
     6661 gttcaatgga gcaggcacag ttgcaggtga gttttgcacg ggcacgtccg ttatccgctg
     6721 cacagaaaga cgagcctggc gcaggacgga agggggaatg cactcaacta gactgaattg
     6781 aacgacgctt gtcttctctc cgttgctgcg cttatgcaga gccgttgaga tagacgcata
     6841 ccaatcgcaa cgctgaacta gtatggactg aagcagcgta tcgatgatag aatccgaaga
     6901 gagaagctgc ccgtcgatat tagagtatac gggagcgagc aactctgagt gggaaaactg
     6961 aaagaccggg ttcgacgcta ccagattgta cagaccctca agagctttct gatgtgcggg
     7021 atggtggaat cgacctcgca aatcaaatcg tttggcggaa agtccgtttg acgcgagcga
     7081 ctgagagaga gcgtcaagcg aggccctggg tgcagtgacc gtagcattta attcatccgt
     7141 aattactgag atatatgcct aataacgtca tcagtggatg caatggccag aaggcaccgt
     7201 tcttacacca gggaattgcg cgagagtctc ttccaggcct ttcttgccag cgcgagacct
     7261 gacagcaact gagctgacat ctgagtctag aagatccagg tctatatatg caccaatcgc
     7321 aacagccagc ctcaacgcga cgcttgcttg acgactcaac gcctcaaggt cagggctcgt
     7381 ggcaaccgac actgctgtga ggaagcccgt gcagaagccc tgcacgcccc cattgttccg
     7441 aacggctctg agaacatccg catgtccccg actaccgttt tcatctgtat agtgcatata
     7501 caggacggta tggaggatga ccgtaaacgg cgtgacgaat gtcgctggaa gttccgagat
     7561 ctctgtggcg tcaggaaact ctgagtggga tagccaacga catagctgcc gtaaagactt
     7621 ctcggcaggg ccagcaacac gttctagtgc agggtcggag atggctaatg cgggccatat
     7681 ctcgggcagc gactcgatcg aggtacgcat ccgatataga cgagggtcga ggagcaacgc
     7741 cgcacgcagt cttgaggccg cttcgacaga cggcaaagta gtctgggaac cgaaaacaag
     7801 agcggatggt gccggagcca t
//
