BGC0000097: ML-449 biosynthetic gene cluster from Streptomyces sp. MP39-85
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Location: 1 - 89,357 nt. (total: 89,357 nt).
This entry is originally from NCBI GenBank FJ872525.1.

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core biosynthetic genes
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General
Compounds
Genes
Polyketide
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0000097
Short description ML-449 biosynthetic gene cluster from Streptomyces sp. MP39-85
Status Minimal annotation: no
A minimal annotation only contains information on the BGC loci and one or more linked chemical product(s)

Completeness: complete
Whether the loci encodes everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • Polyketide (Other)
Loci NCBI GenBank: FJ872525.1
Compounds
  • ML-449
Species Streptomyces sp. MP39-85 [taxonomy]
References
Chemical products information
ML-449
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C29H39N1O3
Chemical database entries
PubCHEM
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • ACO94480.1
 530 - 1102 (-) putative lipoprotein
  • Unknown
Sequence-based prediction
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  • ACO94481.1
 1290 - 1838 (-) hypothetical protein
  • Unknown
Sequence-based prediction
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  • ACO94482.1
  • mlaH
 3783 - 6638 (+) LuxR-type transcriptional regulator
  • Regulation
Sequence-based prediction
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  • ACO94483.1
  • mlaA1
 7108 - 26259 (+) polyketide synthase type I
  • Precursor biosynthesis
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  • ACO94484.1
  • mlaA2
 26297 - 29428 (+) polyketide synthase type I
  • Precursor biosynthesis
Sequence-based prediction
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  • ACO94485.1
  • mlaI
 29667 - 30758 (-) glycine oxidase/FAD-dependent oxidoreductase
  • Precursor biosynthesis
Sequence-based prediction
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  • ACO94486.1
  • mlaC
 30755 - 32842 (-) polyketide synthase type I
  • Precursor biosynthesis
Sequence-based prediction
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  • ACO94487.1
  • mlaU
 32874 - 33437 (-) putative NRPS accessory protein
  • Precursor biosynthesis
Sequence-based prediction
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  • ACO94488.1
  • mlaB
 33434 - 44026 (-) polyketide synthase type I
  • Scaffold biosynthesis
Sequence-based prediction
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  • ACO94489.1
  • mlaJ
 44073 - 45671 (-) putative AMP-dependent acyl-CoA synthetase/ligase
  • Scaffold biosynthesis
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  • ACO94490.1
  • mlaK
 45898 - 46839 (+) acyltransferase
  • Scaffold biosynthesis
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  • ACO94491.1
  • mlaS
 46855 - 47091 (-) peptidyl carrier protein
  • Scaffold biosynthesis
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  • ACO94492.1
  • mlaL
 47209 - 48723 (+) NRPS adenylation domain protein
  • Activation / processing
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  • ACO94493.1
  • mlaM
 48706 - 49302 (-) TetR-type transcriptional regulator
  • Regulation
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  • ACO94494.1
  • mlaN
 49403 - 50974 (+) MFS-type efflux pump
  • Transport
Sequence-based prediction
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  • ACO94495.1
  • mlaO
 51086 - 52321 (-) P450 monooxygenase
  • Tailoring (Monooxygenation)
Sequence-based prediction
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  • ACO94496.1
  • mlaD
 52371 - 62468 (-) polyketide synthase type I
  • Scaffold biosynthesis
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  • ACO94497.1
  • mlaP
 62476 - 63411 (-) putative L-amino acid amidase/proline iminopeptidase
  • Scaffold biosynthesis
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  • ACO94498.1
  • mlaG
 63505 - 69483 (-) polyketide synthase type I
  • Scaffold biosynthesis
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  • ACO94499.1
  • mlaF
 69491 - 79612 (-) polyketide synthase type I
  • Scaffold biosynthesis
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  • ACO94500.1
  • mlaE
 79662 - 84575 (-) polyketide synthase type I
  • Scaffold biosynthesis
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  • ACO94501.1
  • mlaT
 85138 - 85404 (-) SimX2-like protein
  • Unknown
Sequence-based prediction
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  • ACO94502.1
  • mlaQ
 85808 - 86578 (+) thioesterase type II
  • Scaffold biosynthesis
Sequence-based prediction
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  • ACO94503.1
 86721 - 87437 (+) PlsC-type phospholipid/glycerol acyltransferase
  • Unknown
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Polyketide-specific information
Subclass Other
Starter unit Methylmalonyl-CoA
Cyclic? yes
Release type
  • Macrolactamization
Polyketide-synthases
Genes Properties Modules
mlaG
+
mlaA1
+
mlaB
+
mlaA2
+
mlaF
+
mlaD
+
mlaC
+
mlaE 		Synthase subclass: Modular type I
Module 1
Specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
Genes: mlaA1
Core domains: Ketosynthase, Acyltransferase, Thiolation (ACP/PCP)
Module 2
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: mlaA1
Core domains: Ketosynthase, Acyltransferase, Dehydratase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: Unknown
Module 3
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: mlaA1
Core domains: Ketosynthase, Acyltransferase, Dehydratase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: Unknown
Module 4
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: mlaA1
Core domains: Ketosynthase, Acyltransferase, Dehydratase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: Unknown
Module 5
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: mlaA2, mlaC
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: Unknown
Module 6
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: mlaB
Core domains: Ketosynthase, Acyltransferase, Dehydratase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: Unknown
Module 7
Specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
Genes: mlaB
Core domains: Ketosynthase, Acyltransferase, Dehydratase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: Unknown
Module 8
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: mlaD
Core domains: Ketosynthase, Acyltransferase, Dehydratase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: Unknown
Module 9
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: mlaD
Core domains: Ketosynthase, Acyltransferase, Dehydratase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: Unknown
Module 10
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: mlaE
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: Unknown
Module 11
Specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
Genes: mlaF
Core domains: Ketosynthase, Acyltransferase, Dehydratase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: Unknown
Module 12
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: mlaF
Core domains: Ketosynthase, Acyltransferase, Dehydratase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: Unknown
Module 13
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: mlaG
Core domains: Ketosynthase, Acyltransferase, Dehydratase, Ketoreductase, Thiolation (ACP/PCP), Thioesterase
KR-domain stereochemistry: Unknown
Annotation changelog
MIBiG version Submitter Notes
1.0
  • Hidden contributor (ID: MU4LZLKCEF7Q67JF6ZEMKX3R, no GDPR consent given).
  • Submitted
2.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Migrated from v1.4
  • Updated compound(s) information (MIBiG Annotathon)
  • Updated compound(s) information (NPAtlas curation)
3.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Removed gene names of 'No gene ID'
  • Removed duplicate gene name
  • Removed ketoreductase stereochemistry annotation from modules without ketoreductases
  • Sorted modules by module number
  • Corrected module numbering
3.1
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Update chemical activity to schema version 2.11
Detailed domain annotation
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