BGC0000176: difficidin biosynthetic gene cluster from Bacillus velezensis FZB42
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Location: 1 - 69,641 nt. (total: 69,641 nt).
This entry is originally from NCBI GenBank AJ634062.2.

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core biosynthetic genes
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Gene details
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General
Compounds
Genes
Polyketide
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0000176
Short description difficidin biosynthetic gene cluster from Bacillus velezensis FZB42
Status Minimal annotation: no
A minimal annotation only contains information on the BGC loci and one or more linked chemical product(s)

Completeness: complete
Whether the loci encodes everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • Polyketide
Loci NCBI GenBank: AJ634062.2
Compounds
  • difficidin
Species Bacillus velezensis FZB42 [taxonomy]
References
Chemical products information
difficidin
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C31H45O6P1
Chemical database entries
NPAtlas
PubCHEM
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • CAG23974.1
  • difA
 1 - 2376 (+) malonyl CoA [acyl-carrier-protein] transacylase
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  • CAG23975.2
  • difB
 2345 - 3397 (+) putative long-chain fatty acid CoA ligase
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  • CAJ57406.1
  • difC
 3422 - 3694 (+) probable acyl carrier protein
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  • CAJ57407.1
  • difD
 3691 - 5055 (+) acyl CoA synthetase (AMP forming)/AMP acid ligase II
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  • CAG23976.1
  • difE
 5070 - 5807 (+) 3-oxoacyl-(acyl carrier protein) reductase
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  • CAG23977.1
  • difF
 5847 - 18437 (+) polyketide synthase type I
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  • CAG23978.1
  • difG
 18456 - 24752 (+) polyketide synthase type I
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  • CAJ57408.1
  • difH
 24765 - 30518 (+) polyketide synthase type I
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  • CAJ57409.1
  • difI
 30570 - 46184 (+) polyketide synthase type I
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  • CAJ57410.1
  • difJ
 46189 - 53907 (+) polyketide synthase type I
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  • CAJ57411.1
  • difK
 53930 - 60082 (+) polyketide synthase type I
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  • CAG23983.1
  • difL
 60079 - 66294 (+) polyketide synthase type I
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  • CAG23984.1
  • difM
 66370 - 67530 (+) putative cytochrome P450 monooxygenase
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  • CAG23985.1
  • difN
 67588 - 68835 (+) 3-hydroxy-3-methylglutaryl coenzyme A synthase-like
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  • CAG23986.1
  • difO
 68895 - 69641 (+) Enoyl-CoA hydratase
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Polyketide-specific information
Subclass Unknown
Cyclic? no
Polyketide-synthases
Genes Properties Modules
difA
+
difF
+
difG
+
difH
+
difI
+
difJ
+
difK
+
difL 		Synthase subclass:
Trans-acyltansferases: difA
Module 1
Genes: difF
Core domains: Ketosynthase, Dehydratase, Ketoreductase, Thiolation (ACP/PCP)
Scaffold-modifying domains: Methylation
KR-domain stereochemistry: Unknown
Module 2
Genes: difF
Core domains: Ketosynthase, Dehydratase, Ketoreductase
KR-domain stereochemistry: Unknown
Module 3
Genes: difF, difG
Core domains: Ketosynthase, Thiolation (ACP/PCP)
Module 4
Genes: difG
Core domains: Ketosynthase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: Unknown
Module 5
Genes: difG, difH
Core domains: Ketosynthase, Dehydratase, Thiolation (ACP/PCP)
Non-canonical activity:
  • Skipped
Evidence for non-canonical activity:
  • Structure-based inference
Module 6
Genes: difH, difI
Core domains: Ketosynthase, Dehydratase, Ketoreductase, Thiolation (ACP/PCP)
Scaffold-modifying domains: Methylation
KR-domain stereochemistry: Unknown
Module 7
Genes: difI
Core domains: Ketosynthase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: Unknown
Module 8
Genes: difI
Core domains: Ketosynthase, Dehydratase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: Unknown
Module 9
Genes: difI
Core domains: Ketosynthase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: Unknown
Module 10
Genes: difI, difJ
Core domains: Ketosynthase, Dehydratase, Thiolation (ACP/PCP)
Module 11
Genes: difJ, difK
Core domains: Ketosynthase, Dehydratase, Dehydratase
Non-canonical activity:
  • Skipped
Evidence for non-canonical activity:
  • Structure-based inference
Module 12
Genes: difK, difL
Core domains: Ketosynthase, Dehydratase, Thiolation (ACP/PCP), Enoylreductase
Scaffold-modifying domains: Methylation
Module 13
Genes: difL
Core domains: Ketosynthase, Thiolation (ACP/PCP), Thioesterase
Annotation changelog
MIBiG version Submitter Notes
1.0
  • Hidden contributor (ID: FICEQDRJCNKWHHGNCRVDDP5D, no GDPR consent given).
  • Submitted
2.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Migrated from v1.4
  • Updated compound(s) information (NPAtlas curation)
3.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Hidden contributor (ID: 77MNCE2XEGC43DNOUCFLS2KM, no GDPR consent given).
  • Removed invalid NRP information
  • Removed ketoreductase stereochemistry annotation from modules without ketoreductases
  • Reworked PKS modules to match reference paper
  • Updated bioactivity data
3.1
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Update chemical activity to schema version 2.11
Detailed domain annotation
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