LOCUS       BGC0000192             15155 bp    DNA     linear   BCT 14-JUL-2016
DEFINITION  Streptomyces galilaeus aclacinomycin biosynthesis gene cluster,
            partial sequence.
ACCESSION   BGC0000192
VERSION     BGC0000192
KEYWORDS    .
SOURCE      Streptomyces galilaeus
  ORGANISM  Streptomyces galilaeus
            Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
            Streptomyces.
COMMENT     On Aug 15, 2006 this sequence version replaced AF257324.1.
            ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-07 17:39:22
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 15155
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     gene            complement(<1..617)
                     /gene="aknM"
     CDS             complement(<1..617)
                     /codon_start=1
                     /gene="aknM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010:NAD-dependent epimerase/dehydratase (Score: 86.2;
                     E-value: 4.4e-26)"
                     /gene_kind="biosynthetic-additional"
                     /note="dTDP-4-keto-6-deoxyhexose reductase homolog"
                     /product="AknM"
                     /protein_id="AAF70100.1"
                     /transl_table=11
                     /translation="MLGATGCVGRCVSAALAREGHEVLAVARRGGPAVADYLFAGLDVA
                     AVPPAELARLLTGHRVRAVVNVTGGWGTTEEEMRYAHITLVDRLLRALTLMDDRPRLVH
                     VGSIHEYGPVPEPLSIGEDLPPRPATMYARTKLAGSELVLDAASTGRVDALVLRAVNVC
                     GPRTTRASFLGAVAGMLRAADAANPVTLRVADARRDFVDVRDP"
     source          1..15155
                     /db_xref="ATCC:31615"
                     /db_xref="taxon:33899"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces galilaeus"
                     /strain="ATCC 31615"
     protocluster    1..15155
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[8310:9417]"
                     /cutoff="10000"
                     /detection_rule="(t2pks2 or ksIII)"
                     /neighbourhood="20000"
                     /product="PKS-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      8311..9417
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(t2pks2 or ksIII)"
                     /neighbourhood="20000"
                     /product="PKS-like"
                     /protocluster_number="1"
     protocluster    1..15155
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[5443:7935]"
                     /cutoff="20000"
                     /detection_rule="(t2ks and t2clf)"
                     /neighbourhood="35000"
                     /product="T2PKS"
                     /protocluster_number="2"
                     /t2pks_malonyl_elongations="8|9 (Score: 676.9; E-value:
                     1.9e-206)"
                     /t2pks_molecular_weights="propionyl-CoA_8 (Da): 558.573"
                     /t2pks_molecular_weights="propionyl-CoA_9 (Da): 600.609"
                     /t2pks_product_classes="anthracycline"
                     /t2pks_starter_units="propionyl-CoA (Score: 1396.0;
                     E-value: 0)"
                     /tool="antismash"
     proto_core      5444..7935
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(t2ks and t2clf)"
                     /neighbourhood="35000"
                     /product="T2PKS"
                     /protocluster_number="2"
                     /t2pks_malonyl_elongations="8|9 (Score: 676.9; E-value:
                     1.9e-206)"
                     /t2pks_molecular_weights="propionyl-CoA_8 (Da): 558.573"
                     /t2pks_molecular_weights="propionyl-CoA_9 (Da): 600.609"
                     /t2pks_product_classes="anthracycline"
                     /t2pks_starter_units="propionyl-CoA (Score: 1396.0;
                     E-value: 0)"
     cand_cluster    1..15155
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(t2pks2 or ksIII)"
                     /detection_rules="(t2ks and t2clf)"
                     /kind="neighbouring"
                     /product="PKS-like"
                     /product="T2PKS"
                     /protoclusters="1"
                     /protoclusters="2"
                     /tool="antismash"
     subregion       1..15155
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000192"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..15155
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="PKS-like"
                     /product="T2PKS"
                     /region_number="1"
                     /rules="(t2pks2 or ksIII)"
                     /rules="(t2ks and t2clf)"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            complement(656..1261)
                     /gene="aknL"
     CDS             complement(656..1261)
                     /codon_start=1
                     /gene="aknL"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) dTDP_sugar_isom"
                     /gene_kind="biosynthetic-additional"
                     /note="dTDP-4-keto-6-deoxyhexose 3,5-epimerase"
                     /product="AknL"
                     /protein_id="AAF70101.1"
                     /sec_met_domain="dTDP_sugar_isom (E-value: 1.3e-58,
                     bitscore: 187.4, seeds: 19, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQFRELAVSGAFVFTPPVFEDDRGLFTSPFQEPAFVAALGHPLFP
                     VAQSNHSRSRRGTVRGIHYTVTPPGVAKYVYCARGRAIDIVVDIRVGSPTYGRWDSSVL
                     DPEGFRAMYFPVGVGHAFVALEDDTVMSYMLSGSYEAQHEMSLSPLDPALGLPIPQDVA
                     PLLSARDTAAPLLEQVRAEGGLPEYDKCRRIEAALWRP"
     gene            complement(1274..2596)
                     /gene="aknK"
     CDS             complement(1274..2596)
                     /codon_start=1
                     /gene="aknK"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) MGT"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1062:glycosyltransferase, MGT family (Score: 408.4;
                     E-value: 6.5e-124)"
                     /gene_functions="biosynthetic-additional (t2pks) GT (Score:
                     513.1; E-value: 1.4e-156)"
                     /gene_kind="biosynthetic-additional"
                     /note="glycosyl transferase"
                     /product="AknK"
                     /protein_id="AAF70102.1"
                     /sec_met_domain="MGT (E-value: 1.5e-152, bitscore: 498.2,
                     seeds: 193, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKVLFTTFAAKSHMHAQVPLAWALQTAGHEVRIASQPDLAEDITR
                     TGLTAVCVGEPLLLEEQMQRVNEGLGDDAEIMESQAEAGMDMTETRPEMLTWDHVLGVF
                     TSMTAMAFQNSCPERMIDDVVAFAREWQPDLIVWDTLSFAGPVAAQVTGAAHARLLFGL
                     DLLGRMRETFLDLQEERLPEQRDDPLREWLTWTLGRYGAEFEEEVAVGQWTVDPVPPSM
                     RFPVKQPFVPLRYIPYNGQAVIPDWLHEPPKKRRVCLTLGVAHREVLDGDRASIGELVE
                     ALAELDVEVVATLNEKQLAGMELPDNVRAVDFVPLNALLPTCSAVIHHGGSGTFQTALA
                     HGVPQLIVPDMVWDTIHKAKQLERFGAGLYLHDVDNYTAQDLRDHLLRLLEEPSFAENC
                     ARIRREMVGTPSPNDIVPLLEKLTAEHRRDRGARGTVRGEQ"
     gene            complement(2682..4034)
                     /gene="aknE1"
     CDS             complement(2682..4034)
                     /NRPS_PKS="Domain: Polyketide_cyc2 (5-146). E-value: 2e-13.
                     Score: 42.8. Matches aSDomain:
                     nrpspksdomains_aknE1_Polyketide_cyc2.1"
                     /NRPS_PKS="Domain: Polyketide_cyc2 (157-297). E-value:
                     6.8e-10. Score: 31.3. Matches aSDomain:
                     nrpspksdomains_aknE1_Polyketide_cyc2.2"
                     /codon_start=1
                     /gene="aknE1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1128:cyclase/dehydrase (Score: 427.3; E-value:
                     5.9e-130)"
                     /gene_functions="biosynthetic-additional (t2pks) CYC C7-C12
                     (Score: 517.7; E-value: 1.9e-158)"
                     /gene_kind="biosynthetic-additional"
                     /note="putative bifunctional aromatase"
                     /product="AknE1"
                     /protein_id="AAF70103.1"
                     /transl_table=11
                     /translation="MAHEVHTTEHTITVAAAPEAVFGLVERAEDWPHVFPPSLHVEYLE
                     RSGEEERLRIWATANGEVKSWTSRRTLDRAGLRIRFRQEVSQDPVAAMGGEWIVEALPG
                     GGSRVRLLHDFRAVDDLERNVSWILEAIERNSGAELAALESAALRTGTDEAPYTFEDSV
                     RIDAPAQDVRDFLHDAGQWQERLPHVARVLLKEDTPDVQHLEMDTRTPDGSTHTTASVR
                     ICLPDGRITYKQLQTPALMSVHTGEWSVREIPSGCVAVSRHTVVVNAAAVTKVLGASAT
                     TADARTFIRDALGGNSTITMRHAKEYAQRRAATEPVPPAEYASVQRFYARQMRLLDEGE
                     GDAWADTFTEDGTFDQSSFAEPLRGRAAIAAAVRGRPAAPAGTVRRHWLGLPAAWRFPD
                     GSVRTAYDALVVSTEAGAAPVIRLSTSCQDVLVADGDGWLVSHRYVGHDGQ"
     aSDomain        complement(3144..3563)
                     /aSDomain="Polyketide_cyc"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_aknE1_Polyketide_cyc2.2"
                     /domain_subtype="Polyketide_cyc2"
                     /evalue="6.80E-10"
                     /label="aknE1_Polyketide_cyc2.2"
                     /locus_tag="aknE1"
                     /protein_end="297"
                     /protein_start="157"
                     /score="31.3"
                     /tool="antismash"
                     /translation="TFEDSVRIDAPAQDVRDFLHDAGQWQERLPHVARVLLKEDTPDVQ
                     HLEMDTRTPDGSTHTTASVRICLPDGRITYKQLQTPALMSVHTGEWSVREIPSGCVAVS
                     RHTVVVNAAAVTKVLGASATTADARTFIRDALGGNS"
     aSModule        3144..4019
                     /domains="nrpspksdomains_aknE1_Polyketide_cyc2.1"
                     /domains="nrpspksdomains_aknE1_Polyketide_cyc2.2"
                     /incomplete
                     /locus_tags="aknE1"
                     /tool="antismash"
                     /type="unknown"
     aSDomain        complement(3597..4019)
                     /aSDomain="Polyketide_cyc"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_aknE1_Polyketide_cyc2.1"
                     /domain_subtype="Polyketide_cyc2"
                     /evalue="2.00E-13"
                     /label="aknE1_Polyketide_cyc2.1"
                     /locus_tag="aknE1"
                     /protein_end="146"
                     /protein_start="5"
                     /score="42.8"
                     /tool="antismash"
                     /translation="HTTEHTITVAAAPEAVFGLVERAEDWPHVFPPSLHVEYLERSGEE
                     ERLRIWATANGEVKSWTSRRTLDRAGLRIRFRQEVSQDPVAAMGGEWIVEALPGGGSRV
                     RLLHDFRAVDDLERNVSWILEAIERNSGAELAALESA"
     gene            complement(4082..4867)
                     /gene="aknA"
     CDS             complement(4082..4867)
                     /NRPS_PKS="Domain: PKS_KR (7-117). E-value: 9.7e-13. Score:
                     40.2. Matches aSDomain: nrpspksdomains_aknA_PKS_KR.1"
                     /codon_start=1
                     /gene="aknA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001:short-chain dehydrogenase/reductase SDR (Score:
                     267.9; E-value: 1.4e-81)"
                     /gene_functions="biosynthetic-additional (t2pks) KR C9
                     (Score: 484.8; E-value: 1.3e-148)"
                     /gene_kind="biosynthetic-additional"
                     /note="polyketide ketoreductase"
                     /product="AknA"
                     /protein_id="AAF70104.1"
                     /sec_met_domain="adh_short (E-value: 1.3e-40, bitscore:
                     129.3, seeds: 230, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPPASERVAIVTGATSGIGLAVARSLAEGGARVFICARDGDRVAH
                     TVKELREAGHDVDGASCDVRDTARVRAFVQEARDRFGPVDALVNNAGRSGGGHTAQIAD
                     ELWLDVIETNLNSVFRMTREVLTTGGMLERGAGRIVNIASTGGKQGVALGAPYSASKHG
                     VVGFTKALGLELAKTGITVNAVCPGYVETPMAERVRQGYAGAWDITEDEVLERFEAKIP
                     LGRYSTPDEVAGLVGYLLSSTAASITAQAMNVCGGLGNY"
     CDS_motif       complement(4337..4477)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_aknA_0002"
                     /evalue="1.30E-01"
                     /label="PKSI-KR_m1"
                     /locus_tag="aknA"
                     /protein_end="177"
                     /protein_start="130"
                     /score="5.0"
                     /tool="antismash"
                     /translation="MLERGAGRIVNIASTGGKQGVALGAPYSASKHGVVGFTKALGLEL
                     AK"
     aSDomain        complement(4517..4846)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_aknA_PKS_KR.1"
                     /evalue="9.70E-13"
                     /label="aknA_PKS_KR.1"
                     /locus_tag="aknA"
                     /protein_end="117"
                     /protein_start="7"
                     /score="40.2"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C1"
                     /tool="antismash"
                     /translation="VAIVTGATSGIGLAVARSLAEGGARVFICARDGDRVAHTVKELRE
                     AGHDVDGASCDVRDTARVRAFVQEARDRFGPVDALVNNAGRSGGGHTAQIADELWLDVI
                     ETNLNS"
     CDS_motif       complement(4769..4840)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_aknA_0001"
                     /evalue="4.10E-07"
                     /label="PKSI-KR_m1"
                     /locus_tag="aknA"
                     /protein_end="33"
                     /protein_start="9"
                     /score="22.6"
                     /tool="antismash"
                     /translation="IVTGATSGIGLAVARSLAEGGARV"
     gene            5079..5447
                     /gene="aknX"
     CDS             5079..5447
                     /codon_start=1
                     /gene="aknX"
                     /note="polyketide oxygenase"
                     /product="AknX"
                     /protein_id="AAF70105.1"
                     /transl_table=11
                     /translation="MTDHEPGTEGADAVTFVNTFTVHAEPEVFEKEFARTSEFMAQQPG
                     FVRHTLCRHAERPGQYVNVAEWRDLASFRAAVSHDGFRPHADALRALSESRPELYLVRL
                     RREGAPGLDGPASEGEEI"
     gene            5444..6715
                     /gene="aknB"
     CDS             5444..6715
                     /NRPS_PKS="Domain: PKS_KS(Iterative-KS) (5-398). E-value:
                     8.7e-52. Score: 168.2. Matches aSDomain:
                     nrpspksdomains_aknB_PKS_KS.1"
                     /codon_start=1
                     /gene="aknB"
                     /gene_functions="biosynthetic (rule-based-clusters) T2PKS:
                     t2ks"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022:Beta-ketoacyl synthase (Score: 459.8; E-value:
                     1.5e-139)"
                     /gene_functions="biosynthetic-additional (t2pks) KS (Score:
                     725.2; E-value: 4.9e-221)"
                     /gene_kind="biosynthetic"
                     /note="ketosynthase KSI; minimal PKS"
                     /product="AknB"
                     /protein_id="AAF70106.1"
                     /sec_met_domain="t2ks (E-value: 3.7e-228, bitscore: 747.4,
                     seeds: 25, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTARRVVITGLGVIAPGGIGTKAFWERVVSGVSATRTITAFDASG
                     FRSRMAAECDFDGVRSGLTVRDTARLDRATQFAVVAAREALADSGIEIDERNAHRTGVS
                     LGSAVGCTQKLEEEYVARSDGGQRWLVDHAAGTPYLYDYFVPSSMAAEVAWEAGAEGPA
                     ALVSAGCTSGLDSLGHALDLIREGTVDIMIAGGSDAPIAPITVACFDAIKATSPRNDTP
                     EHASRPFDRTRSGFVLGEGAAVLVLEERESALRRGAQIYAEIAGYAGRGNAHHMTGLRP
                     DGLEMSAAITGALDDARIDREAVGYVNAHGTATRQNDIHETAAIKHSLGEHAHRVPVSS
                     IKAVIGHSLGAVGSIEAVASALVIRHGVVPPTAGLHEPDPQLDLDYVPLTARDQATDTV
                     LTVGSGFGGFQSAMVLTSAEGSRS"
     aSDomain        5459..6637
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_aknB_PKS_KS.1"
                     /domain_subtype="Iterative-KS"
                     /evalue="8.70E-52"
                     /label="aknB_PKS_KS.1"
                     /locus_tag="aknB"
                     /protein_end="398"
                     /protein_start="5"
                     /score="168.2"
                     /tool="antismash"
                     /translation="VVITGLGVIAPGGIGTKAFWERVVSGVSATRTITAFDASGFRSRM
                     AAECDFDGVRSGLTVRDTARLDRATQFAVVAAREALADSGIEIDERNAHRTGVSLGSAV
                     GCTQKLEEEYVARSDGGQRWLVDHAAGTPYLYDYFVPSSMAAEVAWEAGAEGPAALVSA
                     GCTSGLDSLGHALDLIREGTVDIMIAGGSDAPIAPITVACFDAIKATSPRNDTPEHASR
                     PFDRTRSGFVLGEGAAVLVLEERESALRRGAQIYAEIAGYAGRGNAHHMTGLRPDGLEM
                     SAAITGALDDARIDREAVGYVNAHGTATRQNDIHETAAIKHSLGEHAHRVPVSSIKAVI
                     GHSLGAVGSIEAVASALVIRHGVVPPTAGLHEPDPQLDLDYVPLTARDQATDT"
     CDS_motif       6467..6538
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_aknB_0001"
                     /evalue="3.40E-02"
                     /label="PKSI-AT-mM_m3"
                     /locus_tag="aknB"
                     /protein_end="365"
                     /protein_start="341"
                     /score="7.2"
                     /tool="antismash"
                     /translation="KAVIGHSLGAVGSIEAVASALVIR"
     gene            6712..7935
                     /gene="aknC"
     CDS             6712..7935
                     /NRPS_PKS="Domain: PKS_KS(Iterative-KS) (64-402). E-value:
                     1.5e-30. Score: 98.3. Matches aSDomain:
                     nrpspksdomains_aknC_PKS_KS.1"
                     /codon_start=1
                     /gene="aknC"
                     /gene_functions="biosynthetic (rule-based-clusters) T2PKS:
                     t2clf"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1093:Beta-ketoacyl synthase (Score: 635.8; E-value:
                     4e-193)"
                     /gene_functions="biosynthetic-additional (t2pks) CLF 8|9
                     (Score: 676.9; E-value: 1.9e-206)"
                     /gene_kind="biosynthetic"
                     /note="KS II; minimal PKS; chain length determinant"
                     /product="AknC"
                     /protein_id="AAF70107.1"
                     /sec_met_domain="t2clf (E-value: 6e-201, bitscore: 657.3,
                     seeds: 26, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSAATVVTGIGVLAPNGIGAEEFWAATLRAESGIGRITRFEPASY
                     PSRLAGEVTGFSARDHIPSRLVPQTDRTTQFALTGSEWALRDSGLSADSLPADERGVVT
                     ASASGGFEFGQRELGHLWGKDPRHVSAYMSFAWFYAVNSGQISIRHDLRGPTGVLVTDQ
                     AGGLDAVAQARRRIRKGTPVMLSGGMDASLCPYGLAAQISAGMLSESDDPARAYRPFDP
                     AADGHVPGEGGAILTLEDGERARARGARSHGEISGYAATFDPRPGSGRPANLDRAIRGA
                     LADAGLTPRDIAFVLADGAGEPEPDRAEARALTDVFGPRGVPVTVPKSMTGRLYAGAVP
                     LDLVTALFALRDGVVPPTVHVDGPDPAYDIDLVTGSAGPSAETRALVLARGRGGFNSAM
                     VVRRPSAA"
     aSDomain        6904..7917
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_aknC_PKS_KS.1"
                     /domain_subtype="Iterative-KS"
                     /evalue="1.50E-30"
                     /label="aknC_PKS_KS.1"
                     /locus_tag="aknC"
                     /protein_end="402"
                     /protein_start="64"
                     /score="98.3"
                     /tool="antismash"
                     /translation="RLVPQTDRTTQFALTGSEWALRDSGLSADSLPADERGVVTASASG
                     GFEFGQRELGHLWGKDPRHVSAYMSFAWFYAVNSGQISIRHDLRGPTGVLVTDQAGGLD
                     AVAQARRRIRKGTPVMLSGGMDASLCPYGLAAQISAGMLSESDDPARAYRPFDPAADGH
                     VPGEGGAILTLEDGERARARGARSHGEISGYAATFDPRPGSGRPANLDRAIRGALADAG
                     LTPRDIAFVLADGAGEPEPDRAEARALTDVFGPRGVPVTVPKSMTGRLYAGAVPLDLVT
                     ALFALRDGVVPPTVHVDGPDPAYDIDLVTGSAGPSAETRALVLARGRGGFNSAMVVR"
     CDS_motif       7369..7407
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_aknC_0001"
                     /evalue="8.40E-03"
                     /label="PKSI-KS_m4"
                     /locus_tag="aknC"
                     /protein_end="232"
                     /protein_start="219"
                     /score="9.6"
                     /tool="antismash"
                     /translation="FDPAADGHVPGEG"
     gene            8039..8314
                     /gene="aknD"
     CDS             8039..8314
                     /NRPS_PKS="Domain: ACP (11-80). E-value: 3.1e-12. Score:
                     38.5. Matches aSDomain: nrpspksdomains_aknD_ACP.1"
                     /codon_start=1
                     /gene="aknD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1129:acyl carrier protein (Score: 104.1; E-value:
                     5.1e-32)"
                     /gene_functions="biosynthetic-additional (t2pks) ACP
                     (Score: 108.3; E-value: 3.4e-34)"
                     /gene_kind="biosynthetic-additional"
                     /note="acyl carrier protein"
                     /product="AknD"
                     /protein_id="AAF70108.1"
                     /sec_met_domain="PP-binding (E-value: 7.3e-10, bitscore:
                     29.3, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSAFTVEELFQIMRECAGEEEAVDLADAAEQEFSLLGYDSLALME
                     AISRVERGLGIDLPEETVGEVLTPAAFVDVVNAELARSAPVVEAAG"
     aSDomain        8072..8278
                     /aSDomain="ACP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_aknD_ACP.1"
                     /evalue="3.10E-12"
                     /label="aknD_ACP.1"
                     /locus_tag="aknD"
                     /protein_end="80"
                     /protein_start="11"
                     /score="38.5"
                     /tool="antismash"
                     /translation="IMRECAGEEEAVDLADAAEQEFSLLGYDSLALMEAISRVERGLGI
                     DLPEETVGEVLTPAAFVDVVNAEL"
     gene            8311..9417
                     /gene="aknE2"
     CDS             8311..9417
                     /codon_start=1
                     /gene="aknE2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     PKS-like: ksIII"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1084:3-oxoacyl-(acyl carrier protein) synthase III
                     (Score: 112.2; E-value: 4.3e-34)"
                     /gene_functions="biosynthetic-additional (t2pks) KSIII
                     (Score: 528.4; E-value: 1.8e-161)"
                     /gene_functions="biosynthetic-additional (t2pks) KSIII
                     AAF70109.1_Aclacinomycin_propionyl-CoA (Score: 729.0;
                     E-value: 0)"
                     /gene_kind="biosynthetic"
                     /note="similar to DpsC"
                     /product="AknE2"
                     /protein_id="AAF70109.1"
                     /sec_met_domain="ksIII (E-value: 3.6e-92, bitscore: 298.6,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTEEHLDPAGGAPTARAPEQDIRIAGCAAWLPPRVPVAKTVAAGL
                     CDEALATATAMVSVAVAQDEPAPEMAARAARTALARDGSDDVSLILHASFFYQGHDLWA
                     PASYVQRVAVGNRCPAIEVGQVSNGGMAALGLAVDHLSAGRPAEAGRRVLVTTGDAFRP
                     PGFDRWRSDPGTFYGDGGTALVLSSGEGFARIRGLATVSAPELEGMHRGDDPFGDTPFS
                     HRPVVDLEACKKDFLASRRVTQVIAASAAAQDAALGQALAAAGAGLADIDRFVLPHMGR
                     KRLRAGFLNRLGIGEDRTTWEWSRGVGHLGAGDQIAGFDHLVGSGSLGPGDLVLWMSVG
                     AGFTYSCAVVEMLERPGWAGTARTAGAA"
     gene            9414..10457
                     /gene="aknF"
     CDS             9414..10457
                     /NRPS_PKS="Domain: PKS_AT (13-295). E-value: 1.6e-71.
                     Score: 232.9. Matches aSDomain:
                     nrpspksdomains_aknF_PKS_AT.1"
                     /codon_start=1
                     /gene="aknF"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PKS_AT"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1021:malonyl CoA-acyl carrier protein transacylase
                     (Score: 275.4; E-value: 1.8e-83)"
                     /gene_functions="biosynthetic-additional (t2pks) AT (Score:
                     480.8; E-value: 5e-147)"
                     /gene_functions="biosynthetic-additional (t2pks) AT
                     AAF70110.1_Aclacinomycin_propionyl-CoA (Score: 667.0;
                     E-value: 0)"
                     /gene_kind="biosynthetic-additional"
                     /note="similar to DpsD"
                     /product="AknF"
                     /protein_id="AAF70110.1"
                     /sec_met_domain="PKS_AT (E-value: 4.4e-72, bitscore: 232.9,
                     seeds: 1682, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTGTAGALPVALLLPGQGSQHRRMAAGLYGHEPVFTEAMDEFFDA
                     AGPEGDPLRDDWLAERPVTDIDHVTRSQPLLFAVDHALGRLVLGRGVRPAALLGHSIGE
                     LAAATLAGVFAPRDAAGLVLDRIRRLSAAPPGGMLAVAASTAEVAPYLRGDVVVGAVNA
                     PRQTVLAGPDGPLDEVDRALREAGFVCRRVPSLSAFHSPVLEPACRGAAPLFAAACKHP
                     PAVPVHSAYTAAPLTESDIDDPAFWARQPVAPVLFWPALEGLLATGDHLLVEVGPGQGL
                     SQLVRRHPAVRRGGSAVVSLLPARPGPPEADRAAVAAATEQITAAGRQAAPASADHGRP
                     SRQAAAG"
     CDS_motif       9450..9500
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_aknF_0001"
                     /evalue="4.20E-04"
                     /label="PKSI-AT-mM_m1"
                     /locus_tag="aknF"
                     /protein_end="29"
                     /protein_start="12"
                     /score="13.0"
                     /tool="antismash"
                     /translation="LLPGQGSQHRRMAAGLY"
     aSDomain        9453..10298
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_aknF_PKS_AT.1"
                     /evalue="1.60E-71"
                     /label="aknF_PKS_AT.1"
                     /locus_tag="aknF"
                     /protein_end="295"
                     /protein_start="13"
                     /score="232.9"
                     /specificity="consensus: mal"
                     /specificity="PKS signature: Malonyl-CoA"
                     /specificity="Minowa: mal"
                     /tool="antismash"
                     /translation="LPGQGSQHRRMAAGLYGHEPVFTEAMDEFFDAAGPEGDPLRDDWL
                     AERPVTDIDHVTRSQPLLFAVDHALGRLVLGRGVRPAALLGHSIGELAAATLAGVFAPR
                     DAAGLVLDRIRRLSAAPPGGMLAVAASTAEVAPYLRGDVVVGAVNAPRQTVLAGPDGPL
                     DEVDRALREAGFVCRRVPSLSAFHSPVLEPACRGAAPLFAAACKHPPAVPVHSAYTAAP
                     LTESDIDDPAFWARQPVAPVLFWPALEGLLATGDHLLVEVGPGQGLSQLVRRHPAVRRG
                     G"
     CDS_motif       9684..9791
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_aknF_0002"
                     /evalue="1.40E-16"
                     /label="PKSI-AT-M_m3"
                     /locus_tag="aknF"
                     /protein_end="126"
                     /protein_start="90"
                     /score="52.6"
                     /tool="antismash"
                     /translation="GVRPAALLGHSIGELAAATLAGVFAPRDAAGLVLDR"
     CDS_motif       9891..9926
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_aknF_0003"
                     /evalue="9.10E-04"
                     /label="PKSI-AT-M_m5"
                     /locus_tag="aknF"
                     /protein_end="171"
                     /protein_start="159"
                     /score="12.5"
                     /tool="antismash"
                     /translation="AVNAPRQTVLAG"
     gene            complement(10511..11371)
                     /gene="aknG"
     CDS             complement(10511..11371)
                     /codon_start=1
                     /gene="aknG"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PCMT"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089:methyltransferase (Score: 117.4; E-value:
                     1.2e-35)"
                     /gene_functions="biosynthetic-additional (t2pks) MET
                     carboxy_O (Score: 431.2; E-value: 2.7e-132)"
                     /gene_kind="biosynthetic-additional"
                     /note="aklanonic acid methyl transferase"
                     /product="AknG"
                     /protein_id="AAF70111.1"
                     /sec_met_domain="PCMT (E-value: 4.3e-12, bitscore: 36.2,
                     seeds: 9, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDTSTYKSEVAAAFNSAAATYDRLGVEFFTPMGRRLVERAAPAAG
                     ARVLDVGCGRGACVFPAAERVGPEGRVVGIDVAEAMIEEATKEAALRDAAMVELRVMDG
                     EHPDFAPHSFDVVLGSYSVIFLPDAPTALARYAPLLSPGGRLAFTSPVFSDDTFPFLPP
                     VFTELIPRELLLDLPPQWQPEQLQQRFNSWLADPRDLTRTLERCGFQEVTVVDEPVTLV
                     AESGLAWVDWSHTQGMRLLWNHLAQDKRQQLRERLITSLDAMRDGDGPLTIDTPVRYVT
                     ATVRN"
     gene            11570..12004
                     /gene="aknH"
     CDS             11570..12004
                     /codon_start=1
                     /gene="aknH"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF07366"
                     /gene_functions="biosynthetic-additional (t2pks) CYC C2-C19
                     (Score: 289.9; E-value: 5.9e-90)"
                     /gene_kind="biosynthetic-additional"
                     /note="aklanonic acid methyl ester cyclase"
                     /product="AknH"
                     /protein_id="AAF70112.1"
                     /sec_met_domain="PF07366 (E-value: 2.1e-37, bitscore:
                     117.8, seeds: 29, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSEQIAAVRRMVEAYNTGKTDDVADYIHPEYMNPGTLEFTSLRGP
                     ELFAINVAWVKKTFSEEARLEEVGIEERADWVRARLVLYGRHVGEMVGMAPTGRLFSGE
                     QIHLLHFVDGKIHHHRDWPDYQGTYRQLGEPWPETEHRRP"
     gene            12048..12890
                     /gene="aknI"
     CDS             12048..12890
                     /codon_start=1
                     /gene="aknI"
                     /gene_functions="regulatory (smcogs)
                     SMCOG1041:transcriptional regulator, SARP family (Score:
                     308.3; E-value: 2.2e-93)"
                     /gene_kind="regulatory"
                     /note="regulator"
                     /product="AknI"
                     /protein_id="AAF70113.1"
                     /transl_table=11
                     /translation="MEFGVLGPLYVRVNGATTAPSAPKLRNVLAMLLVHAGQVVPVPSL
                     VRDLWDEDPPVSGLTTLQTYILNLRKMFSAVTGLTTAEVARDVLVTRAGGYALAAEAGV
                     LDIHRYQRLVTSGREALARGDDAAGVRDLNEALLLWRGPALADVPAGRVLESRRRQLEE
                     SRLAAFEYLVDVELRLGMYREVLAELAALTVENPLHEGLHGQYMWALHLSGRRAQALQV
                     FHRLRGALVAGLGLEPGPQVQRLHQAVLNADTAFEPRFTVRRTAVAASATAFGGGRSY"
     gene            13513..15150
                     /gene="aknOx"
     CDS             13513..15150
                     /codon_start=1
                     /gene="aknOx"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1138:FAD linked oxidase domain protein (Score: 474.9;
                     E-value: 6.3e-144)"
                     /gene_kind="biosynthetic-additional"
                     /product="aclacinomycin oxidoreductase"
                     /protein_id="ABI15166.1"
                     /transl_table=11
                     /translation="MFVLNEFTRRGFLGTAAAVGGTTVVTTALGGAPAAQAAVPEAADG
                     GACGARTALVKVDRVDRRYQDLVTRGFNGRFRGRPDVVYVVHTADQVVDAVNQAMAAGQ
                     RIAVRSGGHCFEGFVDDPAVRAVIDMSQMRQVFYDSGKRAFAVEPGATLGETYRALYLD
                     WGVTIPAGVCPQVGVGGHVLGGGYGPLSRRDGVVADHLYAVEVVVVDASGRARKVVATS
                     AADDPNRELWWAHTGGGGGNFGIVTRYWFRTPGATGTDPSQLLPKAPTSTLRHIVTWDW
                     SALTEEAFTRIIDNHGAWHQSNSAAGTPYASMHSVFYLNSRAAGQILLDIQIDGGLDGA
                     EALLNDFVAAVNEGTGVEPAVQRSTEPWLRATLANKFDTGGFDRTKSKGAYLRKPWTAA
                     QAATLYRHLSADSQVWGEVSLYSYGGKVNSVPETATATAQRDSIIKVWMSATWMDPAHD
                     DANLAWIREIYREIFATTGGVPVPDDRTEGTFINYPDVDLVDERWNTSGVPWYTLYYKG
                     NYPRLQKVKARWDPRDVFRHALSVRPPG"
ORIGIN
        1 ggatcacgca catcgacgaa gtcccgccgc gcgtccgcga cacgcagcgt caccgggttc
       61 gccgcgtcgg cggcccgcag catgccggcg accgccccga ggaaactggc ccgggtggtg
      121 cggggaccgc acacgttcac cgcgcgcagc accagggcgt ccacccggcc ggtgctggcc
      181 gcgtcgagca ccagctcgga gccggccagc ttggtcctgg cgtacatcgt cgccggccgg
      241 ggtggaagat cctcgccgat ggagaggggc tcgggcaccg gcccgtactc gtggatggaa
      301 cccacatgga ccaggcgcgg ccggtcgtcc atcagtgtca gggcccgcag cagccggtcg
      361 accagggtga tgtgcgcgta ccgcatctcc tcctcggtcg tgccccagcc gcccgtcacg
      421 ttgaccacgg cgcgcacccg gtgcccggtg agcagccgcg ccagctcggc aggggggacc
      481 gccgccacgt ccaggccggc gaagaggtaa tccgccaccg cgggcccgcc gcgccgggcc
      541 acggcgagca cctcgtgccc ctcgcgcgcc agagcggcac tcacacagcg tccgacacag
      601 ccggtggccc cgagcaccgc gatccgcgat cccgcggccg gcggccggcc cccgctcagg
      661 gccgccacag tgcggcctcg atccgacggc acttgtcgta ctccggcagg ccgccctccg
      721 cccgcacctg ctcgagcagg ggcgcggccg tgtcccgcgc ggacagcagg ggtgccacgt
      781 cctgggggat cggcaggccg agcgcggggt cgagcgggga gagggacatc tcgtgctgcg
      841 cctcgtagct tcccgagagc atgtacgaca tcaccgtgtc gtcctccagg gcgacgaagg
      901 cgtgcccgac gccgaccggg aagtacatgg cgcgaaaccc ctccgggtcg agcaccgagg
      961 agtcccagcg gccgtaggtg ggcgagccga cgcggatgtc gaccacgatg tcgatggcgc
     1021 ggccccgggc gcagtacacg tacttcgcca cgcccggcgg ggtgaccgtg tagtggatgc
     1081 cccgtacggt gccgcgccgc gacctgctgt ggttggactg cgcgaccggg aacagggggt
     1141 ggcccagtgc cgccacgaag gcaggttcct ggaacggcga ggtgaacaga ccgcggtcgt
     1201 cctcgaagac gggcggagtg aagacgaacg cgccggagac ggcgagttcg cggaactgca
     1261 cggcggaatc catctactgc tcccctcgga cggtgccacg ggcacctcgg tcacggcggt
     1321 gttccgcggt gagcttctcc agcagcggga cgatgtcgtt ggggctcggc gtgcccacca
     1381 tctcccggcg gatacgcgcg cagttctctg cgaacgacgg ctcctccagc agccgcagca
     1441 ggtggtcgcg caggtcctga gcggtgtagt tgtcgacgtc gtgcaggtag agccccgccc
     1501 cgaaccgctc gagctgcttc gccttgtgga tcgtgtccca gaccatgtcg ggcacgatga
     1561 gctgcgggac accatgcgcc agcgccgtct ggaaggtgcc ggagccaccg tggtggatga
     1621 ccgcggagca ggtcggcagc agcgcgttga gcgggacgaa gtcgacggcc ctgacgttgt
     1681 ccggcagttc catcccggcg agctgcttct cgttgagcgt cgcgaccacc tcgacgtcca
     1741 gttccgcgag cgcctcgacg agttcgccga tcgaggcgcg gtcgccgtcc agcacctcgc
     1801 ggtgggccac ccccagcgtc agacagacgc ggcgcttctt cggcggctcg tgcagccagt
     1861 ccgggatgac ggcctgcccg ttgtagggga tgtaccgcag tggcacgaac ggctgcttca
     1921 ccgggaaccg catcgacggc ggcaccgggt cgacggtcca ctgaccgacc gccacctcct
     1981 cctcgaactc ggcgccgtag cggccgagcg tccaggtcag ccactcgcgc agcgggtcgt
     2041 cccgctgctc gggcagccgc tcctcctgaa ggtcgaggaa ggtctcccgc atccgcccca
     2101 gcagatcgag gccgaacagc agccgggcgt gggcggcgcc ggtgacctgc gcggccaccg
     2161 gccccgcgaa ggacagcgtg tcccagacga tcaggtcggg ctgccactcg cgggcgaacg
     2221 cgaccacgtc gtcgatcatg cgctcagggc aggagttctg gaaggccatc gccgtcatcg
     2281 aggtgaacac accgaggacg tggtcccagg tcagcatctc gggacgggtc tccgtcatgt
     2341 ccatcccggc ttccgcctgg ctctccatga tctcggcgtc gtcgcccagg ccctcgttga
     2401 cccgctgcat ctgctcctcc aggagcagcg gctcaccgac acacacggcg gtcagtccgg
     2461 tacgggtgat gtcctccgcc aggtccggct ggctggcgat acggacctcg tggcccgcgg
     2521 tctggagggc ccaggccagc ggaacctggg catgcatatg ggacttcgcg gcgaacgtgg
     2581 tgaacaggac cttcatcgat ctccctcttc gcaggtggtg gtggcggccg cgggaggccg
     2641 gcgagggctc cggcggccgc cgccgtccgg gacggcgggc ttcactgccc gtcgtggccg
     2701 acgtagcggt ggctcaccag ccagccgtcc ccgtcggcga cgaggacgtc ctggcacgag
     2761 gtgctcagcc ggatcaccgg agcggcgccc gcctcggtcg agacgaccag ggcgtcgtag
     2821 gccgtgcgca ccgaaccgtc cgggaaccgc caggcggccg gcaggcccag ccagtgccgg
     2881 cgcaccgtgc cggcgggcgc cgcgggccgg ccgcgcaccg ccgccgcgat cgccgcccgg
     2941 ccgcgcagcg gctcggcgaa cgagctctgg tcgaaggtcc cgtcctcggt gaaggtgtcc
     3001 gcccaggcat ccccctcgcc ctcgtcgagg agccgcatct gacgggcgta gaaccgctgg
     3061 accgaggcgt actcggccgg cggcaccggc tccgtcgcgg cccgccgctg cgcgtactcc
     3121 ttggcgtgcc gcatggtgat cgtgctgttg ccgccgagcg cgtcccggat gaaggtgcgg
     3181 gcgtcggcgg tcgtcgcgga ggcaccgagg accttggtga cggccgccgc gttgacgacc
     3241 acggtgtgcc gggagacggc cacgcagccg gacgggatct cccgtaccga ccactccccg
     3301 gtgtgcacgg acatcagggc cggcgtctgc agctgcttgt acgtgatccg cccgtcgggc
     3361 aggcagatcc gcaccgaagc cgtggtgtgc gtcgaaccgt cgggggtgcg ggtgtccatc
     3421 tccaggtgct ggacgtcggg cgtgtcctcc ttcagcagca cccgggcgac gtgcggcagc
     3481 cgttcctgcc actgccccgc gtcgtgcagg aagtcccgca cgtcctgggc cggggcgtcg
     3541 atgcgcaccg agtcctcgaa ggtgtagggc gcctcgtcgg tgccggtgcg cagcgcggcg
     3601 gactccagcg cggccagctc ggccccgctg ttgcgctcga tcgcctccag gatccagctg
     3661 acgttgcgtt ccaggtcgtc gacggcccgg aagtcgtgca gcagccggac ccggctgccg
     3721 ccgccgggga gcgcctcgac gatccactcc ccgcccatcg cggctacggg atcctgggag
     3781 acctcctggc ggaaccggat ccgcagtccc gcgcggtcca gggtgcgccg tgaggtccag
     3841 gacttgacct cgccgttggc ggtggcccag atgcgcagcc gctcctcctc gcccgaccgc
     3901 tccagatact ccacgtgcag gctcggcgga aagacgtgcg gccagtcctc cgcccgctcc
     3961 accaggccga agacggcctc cggggcggcg gccacggtga tcgtgtgttc ggtcgtgtgc
     4021 acctcgtgcg ccatgctgat cccctctgtc tgcccgaggc cgcgcacggc cgggcccgct
     4081 gtcagtagtt gccgagcccg ccgcagacgt tcatcgcctg ggcggtgatc gacgcggccg
     4141 tcgaggagag caggtagccg acgagtccgg cgacctcgtc cggcgtggag tagcggccga
     4201 gggggatctt cgcctcgaac ctctccagca cctcgtcctc ggtgatgtcc caggcgccgg
     4261 cgtatccctg gcgcacccgc tcggccatcg gtgtctcgac gtaacccggg cagaccgcgt
     4321 tgacggtgat gcccgtcttc gccagctcga ggcccagggc cttggtgaag ccgaccacgc
     4381 cgtgcttcga cgccgagtac ggcgccccca gggccacgcc ctgcttgccg cccgtcgagg
     4441 cgatgttgac gatccggccg gcgccgcgct ccagcatccc gccggtcgtc agcacctcgc
     4501 gggtcatccg gaagacgctg ttgaggttgg tctcgatcac gtcgagccac aactcgtcgg
     4561 caatctgcgc ggtgtgcccg ccgccgctgc ggccggcgtt gttcaccagg gcgtcgacgg
     4621 gcccgaaccg gtccctggcc tcctggacga aggcgcgtac ccgcgcggtg tcgcgtacgt
     4681 cgcaggaggc gccgtcgacg tcgtggcccg cctcgcgcaa ctccttgacg gtgtgggcga
     4741 cccggtcgcc gtcgcgggcg cagatgaaga cccgcgcacc gccttcggcc aggctcctgg
     4801 ccacggcgag accgatgccg ctggtggcac ccgtgacgat ggcgacgcgt tctgatgcag
     4861 gcggcatgac ggctcctcag cggtccttgt ggtgcggtgc cggacgactg cgagggtgtc
     4921 agcggccgct tgaaatgcgc tcgacccccg gcccggcccg cgctccatcg gccttccacc
     4981 cgcctccgag accgggcccg cagcttggta cgagacgaac gaacgcacgg tgtcaagcga
     5041 cgccgggtca caccgaccgg ccaatgagga ggccatcgat gacggatcat gagccaggca
     5101 ccgagggggc cgacgccgtg accttcgtga acaccttcac cgtgcacgcc gaacccgagg
     5161 tgttcgagaa ggagttcgcg cggacgtcgg agttcatggc acagcagccg ggtttcgtcc
     5221 ggcacacgct ctgccggcac gcggagcggc ccggccagta cgtcaacgtc gccgagtggc
     5281 gggacctcgc ctcgtttcgc gcggccgtct cgcacgacgg cttccggccg cacgccgacg
     5341 cgctgcgcgc gctgagcgag agccggcccg aactgtatct ggtacggctg cgccgcgagg
     5401 gcgccccggg tctcgacggt ccggcgtccg aaggggagga gatatgacgg cccgccgggt
     5461 ggtgatcacc ggcctgggag tgatcgcccc gggtggcatc ggcaccaagg cgttctggga
     5521 acgcgtcgtc tccggcgtct ccgcgaccag gaccatcacg gccttcgacg cgtccgggtt
     5581 ccgctcccgg atggccgcgg agtgcgactt cgacggcgtc cgctcgggcc tgaccgtccg
     5641 tgacaccgca cggctggacc gtgccaccca gttcgcggtg gtcgccgcgc gcgaggcgct
     5701 cgccgacagc ggcatcgaga tcgacgagag gaacgcccac cgcaccgggg tcagcctcgg
     5761 ctccgccgtg gggtgcaccc agaagctcga ggaggagtac gtcgcgcgca gtgacggcgg
     5821 gcagcggtgg ctggtcgatc acgccgccgg cacaccgtac ctgtacgact acttcgtgcc
     5881 gagttcgatg gcggccgaag tggcgtggga ggccggcgcc gagggccccg ccgcgctcgt
     5941 ctcggcgggc tgcacctccg gtctcgactc cctcgggcac gcgctcgacc tcatcaggga
     6001 gggcaccgtc gacatcatga tcgccggtgg cagcgacgcg cccatcgcac ccatcaccgt
     6061 ggcctgtttc gacgccatca aggcgacctc gccgcgcaac gacaccccgg agcacgcctc
     6121 gcggcccttc gacaggaccc gcagcggctt cgtgctcggc gagggtgcgg ccgtactcgt
     6181 cctggaggag cgggagtccg ccctgcggcg cggagcacag atctacgccg agatcgcggg
     6241 ctacgccggg cgtggcaacg cccaccacat gaccggactg cgccccgacg gactggagat
     6301 gtccgccgcc atcaccggcg ccctcgacga cgcccgcatc gaccgcgaag ccgtcggtta
     6361 cgtcaacgcg cacggtacgg ccacccggca gaacgacatc cacgagacgg ccgcgatcaa
     6421 gcacagcctg ggcgagcacg cccaccgggt cccggtcagc tccatcaagg cggtcatcgg
     6481 tcactcgctc ggcgccgtcg gctccatcga ggccgtcgcc tccgccctgg tgatccggca
     6541 cggagtggtg ccgcccacgg cgggactgca cgagcccgat ccccagctcg acctcgacta
     6601 cgttccgctg accgcgcgcg atcaggccac cgacaccgtg ctgacggtgg gcagcggatt
     6661 cggcggattc cagagcgcca tggtgctcac cagcgccgaa gggagccgct catgagtgcc
     6721 gcgaccgtgg tcaccggaat cggtgtgctc gcgcccaacg ggatcggggc ggaggagttc
     6781 tgggcggcca ccctgcgcgc cgagagcggc atcggccgca tcacccgctt cgagcccgcc
     6841 tcgtatccct cccggctggc cggcgaggtc acggggttct cggcacggga ccacataccg
     6901 agccggctcg tcccgcagac cgaccgcacc acgcagttcg ccctgacggg ctccgagtgg
     6961 gcactgcggg actccggact gtccgccgac agcctgccgg cggacgagcg cggggtggtc
     7021 accgccagcg cctccggcgg gttcgagttc ggccagcgcg aactgggcca tctgtggggc
     7081 aaggaccccc ggcacgtcag cgcgtacatg tccttcgcct ggttctacgc cgtcaactcc
     7141 ggtcagatct ccatccggca cgatctgcga ggccccaccg gagtgctcgt caccgaccag
     7201 gcgggcggtc tcgacgcggt ggcacaggcc aggcgccgga tccgcaaggg caccccggtc
     7261 atgctgtccg gcggcatgga cgcctcgctg tgcccctacg ggctcgcggc ccagatctcc
     7321 gccggaatgc tcagcgagag cgacgacccg gcccgcgcct accggccgtt cgacccggcg
     7381 gccgacggcc atgtgccggg cgagggcggg gcgatcctga ccctggagga cggggaacgg
     7441 gcgcgggcgc gcggggcacg cagccacggg gagatcagcg ggtacgccgc caccttcgac
     7501 ccccgtcccg gctcgggccg gcccgccaac ctggaccggg cgatccgggg cgcgctggcc
     7561 gacgccgggc tgaccccgcg ggacatcgcc ttcgtcctcg cggacggcgc cggtgaaccg
     7621 gaacccgacc gggccgaggc gcgggcgctc accgacgtct tcggcccccg gggcgtaccc
     7681 gtgacggtcc ccaagagcat gacggggcgc ctctacgcgg gtgccgtccc gctcgatctc
     7741 gtcaccgccc tgttcgcact gcgcgacggc gtcgtcccgc cgacggtcca cgtcgacgga
     7801 ccggaccccg cctacgacat cgacctcgtg accggctccg ccggcccgtc cgcggagacg
     7861 cgggcgctgg tcctggcccg gggacgcggc ggcttcaaca gcgccatggt cgtacggcgc
     7921 ccgtcggcgg cctgagccgt caccccggcg gccgaggccg ccgccatccg ctcacccggc
     7981 cggccagtcg gcggtcggcc cccacaccgc ggcccgtgcg gccgcctttg gagacgtcat
     8041 gtccgctttc acggtagaag agcttttcca gatcatgcgc gagtgcgcgg gcgaggagga
     8101 ggccgtcgat ctggccgacg ccgcggagca ggagttctcc ctgctcggct acgactccct
     8161 cgcgctcatg gaggcgatca gcagggtgga gcggggtctc ggcatcgacc tgcccgagga
     8221 gacggtgggc gaggtgctga cgcccgccgc gttcgtcgac gtcgtcaacg ccgaactggc
     8281 ccgcagcgcg cccgtggtgg aggccgccgg atgaccgagg agcacctcga ccccgcgggc
     8341 ggcgctccca ccgcgcgggc cccggagcag gacatccgga tcgcggggtg cgcagcctgg
     8401 ctgccgcccc gcgtgccggt cgcgaagacg gtggctgcgg gactgtgcga cgaggccctg
     8461 gccacggcca ccgccatggt ctcggtggcc gtggcgcagg acgagccggc ccccgagatg
     8521 gcggcgaggg cggcccggac cgccctcgcg cgcgacggct ccgacgacgt gtcgctgatc
     8581 ctgcacgcca gcttcttcta ccaggggcac gacctgtggg cgcccgcctc ctacgtccag
     8641 cgcgtcgccg tcggcaaccg gtgccccgcg atcgaggtcg gccaggtgtc caacggcggc
     8701 atggccgccc tcggcctcgc cgtcgaccac ctgagcgccg ggcggccggc cgaggcgggc
     8761 cggcgcgtcc tggtgacgac cggtgacgcc ttccgcccgc ccggcttcga ccgctggcgc
     8821 agcgatccgg gcaccttcta cggggacggg ggcaccgcgc tggtgctgtc ctcgggggag
     8881 gggttcgccc ggatccgcgg actggccacc gtctccgctc cggagctgga ggggatgcac
     8941 cgcggtgacg accccttcgg ggacacaccg ttcagccacc gtccggtcgt cgacctggaa
     9001 gcctgcaaga aggacttcct cgcctctcgc cgggtgaccc aggtgatcgc cgcgagcgcg
     9061 gccgcgcagg acgcagccct cggccaggcc ctcgcggcgg ccggggccgg actcgcggac
     9121 atcgaccgct tcgtccttcc ccacatgggc cgcaagcggt tgcgggccgg cttcctcaac
     9181 cgcctcggga tcggggagga ccgcaccacc tgggagtgga gccgcggtgt cgggcacctg
     9241 ggggccggtg accagatcgc cggcttcgac cacctggtcg gctccgggag cctcggcccc
     9301 ggcgacctgg tcctgtggat gagcgtgggc gccgggttca cctactcctg cgcggtcgtc
     9361 gagatgctgg aacggcccgg ctgggccggt acggcgcgta cggcaggtgc cgcgtgaccg
     9421 gcaccgcggg ggccctgccg gtcgccctgc tgctgccggg ccagggctcc cagcaccggc
     9481 ggatggccgc ggggctctac gggcacgagc cggtgttcac cgaggccatg gacgagttct
     9541 tcgacgcggc cggccccgag ggcgatccgc tgcgcgacga ctggctggcc gaacggcccg
     9601 tcacggacat cgaccacgtc acccgctcac agccgctgct cttcgccgtg gaccacgcgc
     9661 tggggcggct ggtgctcggc cggggtgtgc gcccggcggc cctcctgggg cacagtatcg
     9721 gggagctggc ggcggccacg ctggccgggg tgttcgctcc gcgggacgcc gccggcctgg
     9781 tgctcgaccg gatacggcgg ctgtcggcgg ctccgccggg cggcatgctc gcggtggccg
     9841 cgtccacggc ggaggtggcc ccgtacctga ggggagacgt cgtggtcggc gcggtcaacg
     9901 ccccgcggca gaccgtgctc gccggacccg acggcccgct ggacgaggtg gaccgtgccc
     9961 tgcgggaggc cgggttcgtc tgccgccggg tgccgtcgct gagcgccttc cacagcccgg
    10021 tgctcgaacc cgcctgccgg ggggcggccc ctctcttcgc cgcggcctgc aagcatccgc
    10081 cggccgtgcc ggtccactcc gcctacaccg ccgccccgct gacggagtca gacatcgacg
    10141 accccgcctt ctgggcgcgg cagccggtgg cgccggtgct gttctggccc gctctggaag
    10201 ggctgctggc caccggcgac catctgctgg tggaggtggg gccgggacaa gggctgtccc
    10261 agctggtccg ccgtcacccg gctgtgcgcc ggggcggcag tgccgtggtg tcgctgcttc
    10321 cggcgcggcc ggggccgccg gaggcggacc gggccgcggt ggccgccgcc acggagcaga
    10381 tcacggcggc ggggcgccag gccgcccccg cgagcgcgga ccacggccgg ccgtcacggc
    10441 aggccgcggc cggctgaccc acggaaagga ccgacgcccc gtccccgtga accgcccggg
    10501 gggcggggcg tcagttccgt acggtcgcgg tgacgtaccg caccggggtg tcgatcgtga
    10561 gcgggccgtc cccgtcccgc atcgcgtcga gcgaggtgat cagccgctcc cgcagctgct
    10621 gacgtttgtc ctgcgccagg tggttccaga gcaggcgcat cccctgggtg tgcgaccagt
    10681 ccacccaggc caggcccgat tcggccacca gagtcacggg ttcgtcgacg accgtcacct
    10741 cttggaagcc gcaccgctcc agtgtgcggg tgaggtcccg cgggtccgcg agccagctgt
    10801 tgaagcgctg ctgaagctgt tcgggctgcc actgcggcgg caggtcgagc agcagctcgc
    10861 gcgggatcag ttcggtgaac accgggggca ggaaggggaa ggtgtcgtcg ctgaacaccg
    10921 ggctggtgaa ggcgagtcgg cccccggggg agagcagcgg cgcgtaacgg gccagcgccg
    10981 tcggggcgtc gggcaggaag atgacgctgt agctgcccag gaccacgtcg aaggagtgcg
    11041 gcgcgaagtc ggggtgctcc ccgtccatca cccgcagctc gaccatggcg gcgtcgcgca
    11101 gcgccgcctc cttcgtggcc tcctcgatca tcgcctcggc gacgtcgatg cccacgacac
    11161 ggccctcggg ccccacgcgt tcggcagccg ggaagacgca cgcgccccgc ccgcagccga
    11221 cgtcgaggac ccgggcgccg gcggccggtg ccgcgcgttc caccagacgc cggcccatcg
    11281 gggtgaagaa ttccacgccg agccggtcgt aggtcgccgc ggcgctgttg aaggcggccg
    11341 ccacttcgga tttgtaggtg gacgtgtcca ctggctctcc cgctctctcg gtggattctc
    11401 cacttttccc ctccgctctc gggcgcgggt gagcgctctt ggagtccgct ggaatgcggc
    11461 tggacaaacg aaaaggagcc cctggagggc ctgttggctc ttgttacggt cgtgccgtct
    11521 cctaagaatt ggttccggca cggtagtgac cgattcagaa ggagcaggcg tgagtgagca
    11581 gatagcggcc gttcggcgca tggtcgaggc gtacaacacc ggaaaaacgg atgatgtggc
    11641 cgactacatc catccggaat acatgaatcc gggaaccctg gaattcacct cgctgcgtgg
    11701 cccggaactc ttcgcgatca atgtcgcgtg ggtcaagaag acgttctccg aggaggcgcg
    11761 gctggaagag gtcggcatcg aggagcgggc cgactgggtg cgcgcccggc tggtgctcta
    11821 cggccggcac gtcggtgaga tggtcggcat ggcgccgacc ggacggctgt tctccggcga
    11881 gcagatccat ctcctgcact tcgtcgacgg gaagatccac caccaccgcg actggcccga
    11941 ctaccagggc acctaccgcc agctcggcga gccgtggccg gagaccgagc accgccgtcc
    12001 ctgaggcggc ttcgacggcg ggaaacggcg ggaaaatggg gggcgcgatg gagttcggtg
    12061 ttctgggacc gctgtacgtg cgggtgaacg gtgcgaccac cgcgccgagc gccccgaaac
    12121 tgcggaacgt gctggcgatg ctgctcgtcc acgcggggca ggtggtgcct gtgccgtcgc
    12181 tggtgcggga cctgtgggac gaggatccgc cggtcagcgg cctgaccacg ctgcagacgt
    12241 acatcctgaa cctgcgcaag atgttctccg cggtcaccgg actgaccacc gcggaggtgg
    12301 cccgcgacgt cctggtgacc cgtgcgggcg gatacgcgct ggccgcggag gccggcgtgc
    12361 tcgacatcca ccgctaccag cgcctcgtga cctccggccg agaggccctg gcgcgcggtg
    12421 acgacgcggc cggggttcgg gacctgaacg aggcgctgct gctgtggcgg ggccccgcgc
    12481 tggcggacgt accggcgggg cgggtgctgg agagcaggcg ccggcagctg gaggagtccc
    12541 ggctcgccgc cttcgaatac ctggtcgacg tggagctgcg gctcggcatg taccgcgagg
    12601 tgctcgccga gctggccgcc ctcacggtgg agaacccgct gcacgagggg ctgcacggcc
    12661 agtacatgtg ggccctgcac ctcagcgggc ggcgggccca ggcgttgcag gtcttccacc
    12721 ggctgcgggg tgcgctggtg gcgggtctcg gcctggagcc gggtccgcag gtgcagcggc
    12781 tgcaccaggc ggtgctgaac gcggacaccg ccttcgaacc ccgcttcacc gtgcgccgga
    12841 ccgcggtcgc cgcgtcggcc acggccttcg ggggcggacg gtcgtactga cgggcgtgac
    12901 cacacgtgat tgcgctgctc gatcaggcgt ggctagggtg cccactagcc aaatttgatt
    12961 agttcggagg ggtcatgccg gagaaggacc agtcgcagcg gacggaacag caggacgagg
    13021 cgcccctgga cagtcccacg gggtgggtgg cggcgcacat ccgccgttac gaccgcagcg
    13081 gcgggaagga ggggcagaag tggtacgggc tcgacacgct gctcctgacc acccgcggcc
    13141 ggaaatcggg ccggctccgc cgcaccgcgg tgatctacgg ccgggacggc ggcaacatca
    13201 tcgtcgtcgg atcgaacggg ggaaagcccg agcacccctt gtggtacctc aacctcgtcg
    13261 cctcgcccga ggcccacgtc cagatcggtg aggaacatct ggatgtgcgg gcgcgcacgg
    13321 cgaccgccga ggagaagccg gatctctgga agctgatgac ggggctcttt ccccagtaca
    13381 agagttacca gaagaagacc gaccgggaaa ttccggtggt cattctggag cccgtcgagc
    13441 agtgatcgtc gacggcccgg aaccgagccg aggccgaatg ctcgagaaaa tttaacggca
    13501 ggtaaggaaa gggtgtttgt gttgaacgaa ttcacgcgcc gcggattcct cggcaccgcg
    13561 gcagcggtcg gcggaaccac cgtggtgacc acggccctgg gcggagcccc ggccgcccag
    13621 gcggccgtcc cggaagcggc ggacggtggc gcgtgcggcg cgcggaccgc gctggtgaag
    13681 gtggaccggg tggaccggcg ctaccaggac ctggtcacca ggggcttcaa cgggcgcttc
    13741 cgcggccggc ccgacgtcgt gtacgtcgtc cacaccgcgg accaggtcgt cgacgcggtg
    13801 aaccaggcca tggccgcggg ccagcggatc gcggtgcgca gcggcgggca ctgcttcgag
    13861 ggcttcgtgg acgatcccgc cgtccgggcc gtcatcgaca tgtcccagat gaggcaggtc
    13921 ttctacgact ccggcaagcg ggcgttcgcc gtggaacccg gcgccaccct cggggagacg
    13981 taccgggccc tgtatctgga ctggggcgtg accatcccgg ccggcgtctg cccccaggtg
    14041 ggcgtcggcg gccatgtgct gggcggaggc tacggtccgc tgtcccgccg ggacggcgtg
    14101 gtggccgacc atctgtacgc ggtcgaggtc gtcgtcgtgg acgcctcggg ccgggcccgc
    14161 aaggtcgtgg ccacgagcgc cgcggacgac cccaaccgag agctgtggtg ggcccacacc
    14221 ggcggcggcg gaggcaactt cgggatcgtc acccggtact ggttccgcac ccccggggcc
    14281 accggcaccg acccgtccca actcctgccc aaggccccca cgtccaccct gcgccacatc
    14341 gtgacctggg actggtccgc tctcaccgag gaggcgttca cccggatcat cgacaaccac
    14401 ggggcctggc accagagcaa cagcgccgcc ggcaccccgt acgccagcat gcacagcgtc
    14461 ttctacctca acagccgtgc ggccggtcag atcctgctgg acatccagat cgacggcggc
    14521 ctggacggcg ccgaggccct gctgaacgac ttcgtggccg cggtcaacga gggcaccggc
    14581 gtcgagcccg ccgtgcagcg gagcacggag ccgtggctgc gcgccacgct ggccaacaag
    14641 ttcgacaccg gcggcttcga ccggaccaag tcgaagggcg cgtatctgcg caagccctgg
    14701 accgccgccc aggccgccac tctctaccgc catctgagcg ccgactccca ggtctgggga
    14761 gaggtctcgc tctactcgta cggaggcaag gtcaactccg tgccggagac ggccacggcc
    14821 acggcccagc gcgactccat catcaaggtg tggatgtcgg cgacctggat ggatcccgcc
    14881 cacgacgatg ccaacctcgc ctggatccgg gagatctacc gcgagatctt cgccaccacc
    14941 ggcggcgttc cggtgcccga cgaccgcacg gagggcacct tcatcaacta tcccgacgtc
    15001 gacctggtcg acgagcggtg gaacacctcg ggagtcccct ggtacaccct ctactacaag
    15061 gggaactacc cgcgcctgca gaaggtcaag gcgcgctggg atccccgtga cgtcttccgg
    15121 cacgcgctgt ccgtgcggcc gcccggctga ccgga
//
