LOCUS       BGC0000280              6033 bp    DNA     linear   BCT 26-JUL-2016
DEFINITION  Pseudomonas fluorescens strain F113 2,4-diacetylphloroglucinol
            biosynthetic gene cluster, partial sequence.
ACCESSION   BGC0000280
VERSION     BGC0000280
KEYWORDS    .
SOURCE      Pseudomonas fluorescens
  ORGANISM  Pseudomonas fluorescens
            Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
COMMENT     ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2022-11-07 18:03:21
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6033
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..6033
                     /db_xref="taxon:294"
                     /mol_type="genomic DNA"
                     /note="biocontrol strain; very efficient against Pythium
                     ultimum"
                     /organism="Pseudomonas fluorescens"
                     /strain="F113"
     subregion       1..6033
                     /aStool="mibig"
                     /contig_edge="True"
                     /label="Polyketide"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..6033
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     CDS             complement(385..1011)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1224:TetR family
                     transcriptional regulator (Score: 167.6; E-value: 4.8e-51)"
                     /gene_kind="regulatory"
                     /product="PhlF"
                     /protein_id="AAM27406.1"
                     /transl_table=11
                     /translation="MARKPSRSSIGSLRSPHTHKAIIISAIETLKECGYSGLSIEAVAR
                     RAGASKPTIYRWWGNKAALIAEVYESESEQIRKEPDRGSFKENLNFLLLNLWKVWRETI
                     CGEAFRCVIAEAQLDPSTLPKLKDEFMERRRELPRKLVENAIQQGELPKDTSRELLLDM
                     IFGFCWYRLLTEQLEVEADINEFTTLLLNGVLCTNSAAVQGAAEA"
     misc_feature    1012..1428
                     /note="intergenic region containing phlF binding site"
     CDS             1429..2511
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1043:hydroxymethylglutaryl-CoA synthase (Score: 237.7;
                     E-value: 3.4e-72)"
                     /gene_kind="biosynthetic-additional"
                     /note="5' region determined by primer extension"
                     /product="PhlA"
                     /protein_id="AAM27405.1"
                     /transl_table=11
                     /translation="MNKVGIVSYGAGIPVCRLKVDDVIQVWKNTDLSLVKGQLGVLERA
                     VLQPDEDVITLGVLAAQRALDKAPPCSLEALYLGTCTNPYDSRASAAIILEMLGCGYDA
                     FCADVQFAGKSGTTALQIAYALVASGMVGNALAVGADTINRNTAPGDLTEWYAGAGAAA
                     LLLGTENVIAHFDASFSCAADVADNIRPQGDRYIRSGMGLGSDKNSIGLEDHTRRAASG
                     LMAKIHAQAGDFDYVVFQQNLVSTPYSLGKHLGFTTAQIEPGIYAQSVGDAGAASPLLG
                     LVNVLDQARPGERILVVSYGFGAGSDAIALTVTDAIEAYQKTNVPLRTLLEDKYYVDYG
                     TSIKYEFKYLRPDYALTAYL"
     CDS             2543..3739
                     /codon_start=1
                     /product="PhlC"
                     /protein_id="AAM27404.1"
                     /transl_table=11
                     /translation="MSARRVAIVSAAYTPKPGSSRVCQTFKEMIVESAYQALNAIKMHP
                     RELQAERYGYHGEGISEYGGLGPTISDALGISPAPTFMSTANCTSSSVSFQMAHQMVAS
                     GEYDIVLCGGFEKMTDHINYAEYIGSSTECEYDYFLGILHTDAFALATAEYFEKFGYAG
                     READVLATFGRQMRIYAHNTPTATRYGVPIPSLEALKSSEACGSMLAWGEASGCAILVA
                     EHLAHRYTTQPVFIRGCAYTGVSHYFGTRYHNPTLQFPGLPKDVGMAVSANSMACAEIA
                     YKKAGITAKDIDVAQVYDLLGAGLIQMESMGVCGPGQAGDFVLEGGIALAGQLPLNTDG
                     GNIGRGHASGCDGILHITELFRQLRGESNNQVKGARIGVSQNLGGYAAHNSVIVLSND"
     CDS             3750..4190
                     /codon_start=1
                     /product="PhlB"
                     /protein_id="AAM27407.1"
                     /transl_table=11
                     /translation="MSLYPEQIHRMTTASMLREWREHGGKYRLEGSRCQDCEEIFFPRR
                     TVCGACNSLNVEPYRCARTGTIQVSAHAENPILAAMGYGETVPRFMAMVRLDDGLVIAS
                     EIVDVVDPRQVVSGAPVRMVIRKHVRESNLAWQYAYKFVLVK"
     CDS             4346..5395
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) T3PKS:
                     Chal_sti_synt_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1024:chalcone and stilbene synthase domain protein
                     (Score: 343.4; E-value: 2e-104)"
                     /gene_kind="biosynthetic"
                     /product="PhlD"
                     /protein_id="AAM27403.1"
                     /sec_met_domain="Chal_sti_synt_N (E-value: 1e-24, bitscore:
                     75.9, seeds: 21, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTLCKPSLLFPQYKITQQQMIEHLEQLHDDHPRMALAKRMIQNT
                     QVNERHLVLPIDELAVHTGFTHRSIVYEREARRMSSIAARQAIENAGLTTDDIRMVAVT
                     SCTGFMMPSLTAHLINDLGLRTSTVQLPIAQLGCVAGAAAINRANDFASRAPDNHVLIV
                     SLEFSSLCYQPQDTKLHAFISAALFGDAVSACVMRADDQAPGFKIAKTGSYFLPDSEHY
                     IKYDVKDSGFHFTLDKAVMNSIKDVAPMMEELNYETFNQHCAQNDFFIFHTGGRKILDE
                     LVLQLDLEPGRVAQSRDSLSEAGNIATVVVFDVLKRQFDSGLVDGATGMLAAFGPGFTA
                     EMAVGKWVA"
     CDS             5521..>6033
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1106:major
                     facilitator transporter (Score: 115.3; E-value: 4.3e-35)"
                     /gene_kind="transport"
                     /product="PhlE"
                     /protein_id="AAM27402.1"
                     /transl_table=11
                     /translation="MEMKYLVARPWGGYERRMITLLSLSFGLVGLDRFIIMPLFPVIMR
                     DLALDYQDLSMLSAILAFAWGGSALFMGAAIKVLGPKKLLVLSITLLSLLAGASALITS
                     LVGLVLLRALMGVCEGAFTPVSIIVTDEVSQPSRRGLNLGIQQALFPIIGLCLGPLLAG
                     VLLEIFGS"
ORIGIN
        1 ggatccctgc gagctgggct tctgtccgga tctgtaccgc gaatccaaaa tagacggcag
       61 cgtcggtgcc ctgatgagcc tggcccacga accgcaagtg ccggtgtcgc tgatggcgca
      121 cttgtttcaa ggaatgcccg gaaggtttgt acctgaacgg agccgttact gggtcggcag
      181 ccaccctgcc atgcagcgtt ttccaggcgc cgagcgagcc gctcagctac tggaagaaaa
      241 cggcctggga gaagtggagc tggaaaccct ggcttatgaa tttgcggtgc atgacttgtg
      301 tgagttcaac cacctggcca gccattctgc cgagccttca tgcacaattc agtggcgcaa
      361 agtaagggca ccgccacccg ataatcaggc ttcggcggcg ccctgaaccg ctgccgaatt
      421 tgtgcacagt accccgttca gcagaagtgt cgtgaattca ttgatatccg cttccacctc
      481 caattgctcg gtcaacagcc ggtaccagca aaatccaaag atcatgtcca ggagcaattc
      541 acgcgacgtg tccttgggca actcgccctg ctgaatggca ttttccacca gcttgcgagg
      601 caactcccta cgtcgctcca tgaattcgtc cttcagtttg ggcagcgtgc tgggatcgag
      661 ttgcgcctca gcgatgacac accgaaaagc ctccccgcaa attgtttctc gccagacctt
      721 ccagagattg agcagcaaga aattgaggtt ctccttgaag gaacctctat caggttcttt
      781 gcgaatctgc tcgctttcac tctcgtagac ctcggcgatc aaggctgcct tgttacccca
      841 ccaccggtag attgtcggtt tgctggcgcc agcgcggcga gccaccgcct cgatgctcag
      901 ccccgagtaa ccgcactcct taagggtttc tatggcagaa ataatgatcg ctttgtgcgt
      961 gtgggggctc ctcaatgagc caatggagct ccgagatggt ttacgggcca taaatcaaaa
     1021 tctccaatat ggcgcttgac aaaagagact cagggtatag gattctctcg aaaagcaatg
     1081 aaacggatcg tttcgttttg tatgtttatt tgatctcagc ttctgcttag cagggggtct
     1141 cttgagcgct tattacctct aaatagggag atatgagaag gaaagtcata aaattttaac
     1201 gctaccttaa cgatacggta cgtttcgtat catacataat gattgtacga aaacaaacct
     1261 attgttcata ggttgaccgg taggttctga cgcttgaacc tgccgattta tgggcatggg
     1321 accgccattt atcactttgc gagcaccaat agatagttct aaatatttca tgcgcctgtc
     1381 tcggctttct gccagcaggt gaaagaacgg aatctggagg aagtacacat gaataaagta
     1441 ggaattgtga gctatggcgc gggcatcccg gtctgccggc tcaaggtgga cgacgtgatc
     1501 caggtgtgga aaaacaccga tttgagcttg gtcaagggtc aactgggcgt tcttgaaagg
     1561 gccgtcctgc aacccgatga agacgtcatt accttgggcg tcctcgccgc gcaacgcgca
     1621 ctggacaaag cgccgccttg ttcccttgaa gcgctttatc ttggcacctg caccaatcct
     1681 tatgactccc gtgcctcggc ggcgatcatc ctggaaatgc tcggctgcgg ctacgacgcc
     1741 ttctgtgcag acgtgcagtt tgccggaaaa tcaggcacca ccgcccttca gattgcatac
     1801 gccctggtag catcgggcat ggtgggcaac gcgttggcgg tgggcgccga tacgatcaac
     1861 cgcaacaccg ctcccggtga tttgacagag tggtacgccg gagcgggcgc cgctgccctg
     1921 ctgttgggta cagaaaatgt gatcgcgcat tttgacgcaa gtttttcttg cgcggcggat
     1981 gtcgctgata acatcaggcc tcaaggggac cgctatatcc ggtcgggaat gggactgggc
     2041 tcggacaaga acagcatcgg ccttgaagac cacactcgcc gcgctgcctc gggactgatg
     2101 gccaagatcc atgcgcaggc cggcgatttc gattatgtcg tcttccaaca gaacctagtg
     2161 tcgaccccct actctctcgg caagcacctg ggattcacga cggcgcaaat cgaaccgggc
     2221 atctatgccc agagtgtcgg tgatgctggg gcagcgagcc ctttgctggg tttggtcaac
     2281 gtcctcgatc aagcgcgtcc cggtgaaaga atcctggtgg tttcctatgg gtttggtgcg
     2341 ggcagcgatg ccattgcctt gaccgtcacc gatgccatcg aagcctatca aaagaccaac
     2401 gttccgctgc gcacgctgct ggaggataag tactacgtgg attacgggac gtcgatcaag
     2461 tacgagttca aatacttgcg gcccgactat gccctgacgg cctatctctg attccaatcc
     2521 atccagcaag gagcactttt ttatgagcgc acgacgcgtt gctatcgttt cggctgccta
     2581 tacccctaag cccggcagct ccagagtttg tcaaactttc aaggagatga tcgtcgagtc
     2641 cgcctaccag gcgctcaacg ccatcaagat gcacccccgc gaacttcagg cagaacggta
     2701 tggctaccac ggtgaaggca tttctgaata cggaggcctg ggcccgacca tttccgatgc
     2761 cctgggtatc agccctgctc ccacgttcat gagtactgcc aactgcacca gcagctcggt
     2821 gtctttccag atggcccacc aaatggtcgc ctcaggtgag tacgacatcg tcctgtgcgg
     2881 cggcttcgaa aaaatgaccg accacatcaa ctacgccgaa tacatcggct ccagcactga
     2941 atgtgaatac gattattttc tgggcatctt acacacagac gcctttgccc ttgcaaccgc
     3001 cgaatatttt gaaaaatttg gctatgcagg acgcgaagcg gatgtattgg caacgtttgg
     3061 gcggcagatg cgtatctatg cccataacac ccccacggct acccgctatg gcgtgccgat
     3121 cccttccctc gaagcactca agagtagcga agcctgtggc tcgatgttgg cctggggtga
     3181 agccagcggt tgcgccatcc tggttgccga acaccttgcg caccgttaca cgacccaacc
     3241 ggtctttatc cgtggctgtg cctacaccgg cgtatcgcac tatttcggca cgcgctatca
     3301 caacccgacg ttgcaatttc cggggctgcc aaaagatgta ggcatggcgg tttccgccaa
     3361 ctcgatggcc tgcgccgaaa tcgcctacaa aaaagccggc atcactgcaa aggatatcga
     3421 tgtggcgcaa gtctacgact tgctgggcgc cgggttgatt caaatggagt ccatgggcgt
     3481 atgtggtcca ggccaggcag gcgactttgt attggaaggc ggcatcgccc ttgctgggca
     3541 acttcctctg aacactgacg gcggcaacat cggacgcggc cacgcctccg gttgcgatgg
     3601 gattttgcac attactgaat tgttccgaca gcttcgcggc gagtccaaca accaagtcaa
     3661 aggcgcccgt attggcgttt cgcaaaacct gggcggttac gccgcgcaca acagtgtcat
     3721 tgtgctttcc aacgattaag gagccgacca tgtcccttta ccctgaacaa attcaccgca
     3781 tgaccaccgc cagcatgttg cgcgaatggc gcgagcacgg cggaaaatac cgtctcgaag
     3841 gcagccgttg tcaggattgc gaagagatct tcttcccccg gcgcacagtg tgtggcgcgt
     3901 gcaactcgtt gaatgtggaa ccgtatcgct gcgcccgcac cggcacgatc caagtcagtg
     3961 cccatgccga gaatccgata ctggcggcca tgggctatgg cgaaaccgtg ccccgcttca
     4021 tggcgatggt gcgcctggat gatggcctgg tgattgcctc ggaaatcgtt gatgtcgtcg
     4081 atccccggca agttgtcagc ggtgcgcctg tgcgcatggt tattcgcaaa catgtccgag
     4141 aaagcaactt ggcctggcaa tatgcctata agtttgtatt ggtgaaataa caaactaggc
     4201 gcccgctaca tcccgctctt tcgataacaa cagtcaagtt ttttggctct ccagcccgct
     4261 ttgattctgt ctttcagggt cgtgctttgc agtcattgcc ttaaatgtgc ttctaacttt
     4321 attggcttct cgccgaggac ttgtcatgtc tactctttgc aaacccagtc tgttgtttcc
     4381 gcaatacaag atcacccagc aacaaatgat cgaacatctt gagcagttgc atgatgatca
     4441 tccacgaatg gcacttgcca aacgaatgat tcaaaacaca caagtgaacg aacggcactt
     4501 ggtcctgcct atcgatgaac tggcggtgca caccggcttc actcaccgca gcatcgttta
     4561 tgagcgcgaa gcccgccgca tgtcatccat cgcggcgcgc caagccattg agaatgccgg
     4621 gctgaccacc gacgacattc gaatggtcgc ggtgacatcg tgcacgggtt tcatgatgcc
     4681 ttcgctgacg gcccacctga tcaatgacct gggcctgcga acctcgaccg tacaactgcc
     4741 catcgctcaa ttgggctgcg tggcaggcgc tgcggcgatc aatcgagcca atgattttgc
     4801 cagccgggcg ccggacaacc atgtcctcat cgtctccctg gagttttcat cgctctgcta
     4861 tcaaccccag gacaccaagt tgcacgcgtt catatcggcg gcgttatttg gcgatgccgt
     4921 atcggcctgc gtcatgcgcg ccgatgacca ggcgcctggt ttcaagatcg ccaagaccgg
     4981 gtcttacttc ctgcctgaca gcgagcacta cattaaatac gacgttaaag acagcggctt
     5041 tcacttcacc ctggacaagg ctgtcatgaa ctccattaaa gatgtcgctc cgatgatgga
     5101 ggaattgaac tacgagacct tcaatcaaca ttgcgcgcaa aatgacttct tcatcttcca
     5161 taccggcgga cggaaaattc ttgatgaact ggtcctgcaa ctggacttgg aacccggccg
     5221 ggtcgcgcag tctcgcgaca gtttgagcga agccgggaac atcgccacgg tggtggtctt
     5281 cgatgtgctc aagcgccagt ttgacagcgg gcttgtcgat ggcgcgacgg gcatgctggc
     5341 ggccttcggc cccggtttca ccgctgaaat ggccgtgggc aagtgggtcg cctgacagac
     5401 cgataaaaca ccgacaccca gccaggggca catctgcccc tggctgctgg cgtatggatc
     5461 gcaggcgttc acctcgttcg agagggcttc gaaagcgcta gggaaagaag gggaaggaac
     5521 atggagatga aatatctggt cgcccgacct tggggcggct atgaaagacg catgatcacg
     5581 cttctgtcgc tgagcttcgg gctggtagga ctggacaggt tcattatcat gccgctgttc
     5641 ccggtgatca tgcgtgactt ggccctggac tatcaggact tgagcatgtt gtcggccatc
     5701 ctggcatttg catggggagg ctctgccctg ttcatgggcg ccgccatcaa ggtactgggc
     5761 cccaagaagt tgctggtgct gtcaatcacc ctgctatcgc tcctggccgg tgcgtctgca
     5821 ttgatcacca gcctggtggg cctggtactc ttgcgcgcct tgatgggcgt ctgtgaaggt
     5881 gccttcactc cagtcagtat cattgtcacc gatgaagtct cgcagccttc ccggcgcgga
     5941 ctcaacctag ggattcaaca ggccttgttc cccatcatcg gcctgtgcct cggaccgctg
     6001 ctggcgggcg tcttgctgga aatattcgga tcc
//
