BGC0000350: ET-743 biosynthetic gene cluster from uncultured organism
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Location: 1 - 35,392 nt. (total: 35,392 nt).
This entry is originally from NCBI GenBank HQ609499.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
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Gene details
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General
Compounds
Genes
NRP
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0000350
Short description ET-743 biosynthetic gene cluster from uncultured organism
Status Minimal annotation: no
A minimal annotation only contains information on the BGC loci and one or more linked chemical product(s)

Completeness: incomplete
Whether the loci encodes everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • NRP (Beta-lactam)
Loci NCBI GenBank: HQ609499.1
Compounds
  • ET-743
Species uncultured organism [taxonomy]
References
Chemical products information
ET-743 [synonyms: yondelis, ecteinascidin 743, trabectedin]
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C39H43N3O11S1
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • ETU_000001
  • ADQ55468.1
 1 - 1167 (-) acetyl-CoA carboxylase biotin carboxylase subunit
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  • ETU_000002
  • ADQ55469.1
 1215 - 1673 (-) acetyl-CoA carboxylase biotin carboxyl carrier protein subunit
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  • ETU_000003
  • ADQ55470.1
 2074 - 2862 (-) Mg-dependent DNase
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  • ETU_000004
  • ADQ55471.1
 2954 - 3868 (-) DNA polymerase III delta prime subunit
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  • ETU_000005
  • ADQ55472.1
 4283 - 5089 (+) hypothetical 29 kDa protein
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  • ETU_000006
  • ADQ55473.1
 5154 - 6239 (+) SAM-dependent methyltransferase
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  • ETU_000007
  • ADQ55474.1
 6255 - 8201 (+) NRPS
  • Scaffold biosynthesis
Sequence-based prediction
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  • ETU_000008
  • ADQ55475.1
 8319 - 12653 (+) NRPS
  • Scaffold biosynthesis
Sequence-based prediction
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  • ETU_000009
  • ADQ55476.1
 12734 - 18106 (+) NRPS
  • Scaffold biosynthesis
Sequence-based prediction
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  • ETU_000010
  • ADQ55477.1
 18099 - 20105 (+) pyruvate dehydrogenase E1 component
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  • ETU_000011
  • ADQ55478.1
 20074 - 21171 (+) pyruvate dehydrogenase E2 component
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  • ETU_000012
  • ADQ55479.1
 21249 - 22742 (+) FAD-binding monooxygenase
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  • ETU_000013
  • ADQ55480.1
 22720 - 23382 (-) O-methyltransferase family 3
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  • ETU_000014
  • ADQ55481.1
 23389 - 23868 (-) catechol hydroxylase
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  • ETU_000015
  • ADQ55482.1
 24066 - 24407 (-) transcriptional regulator MerR family
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  • ETU_000016
  • ADQ55483.1
 24434 - 26680 (-) penicillin acylase
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  • ETU_000017
  • ADQ55484.1
 26897 - 28357 (-) aspartyl glutamyl-tRNA Asn Gln amidotransferase subunit B
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  • ETU_000018
  • ADQ55485.1
 28368 - 29549 (-) aspartyl glutamyl-tRNA Asn Gln amidotransferase subunit A
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  • ETU_000019
  • ADQ55486.1
 29878 - 30186 (-) aspartyl glutamyl-tRNA Asn Gln amidotransferase subunit C
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  • ETU_000020
  • ADQ55487.1
 30383 - 31828 (+) peptidase U62
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  • ETU_000021
  • ADQ55488.1
 31961 - 32491 (+) shikimate kinase I
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  • ETU_000022
  • ADQ55489.1
 32712 - 33116 (-) DnaK suppressor protein
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  • ETU_000023
  • ADQ55490.1
 33226 - 34143 (-) putative drug_metabolite transporter superfamily protein
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  • ETU_000024
  • ADQ55491.1
  • polA
 34413 - 35036 (+) DNA polymerase I
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  • ETU_000025
  • ADQ55492.1
 35042 - >35392 (+) hypothetical protein
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NRP-specific information
Subclass Beta-lactam
Cyclic? yes
Release type
  • Reductive release
NRP-synthases
Gene Modules
ETU_000009
Module 0
Specificity: fatty acid
Evidence for specificity:
  • Structure-based inference
Condensation domain type: Unknown
Module 1
Specificity: cysteine
Evidence for specificity:
  • Sequence-based prediction
Condensation domain type: Unknown
ETU_000007
Module 2
Specificity: glycolic acid
Evidence for specificity:
  • Sequence-based prediction
Condensation domain type: Unknown
ETU_000008
Module 3
Specificity: 3-hydroxy-O-methyl-5-methyltyrosine
Evidence for specificity:
  • Structure-based inference
Non-canonical activity:
  • Iterated
Evidence for non-canonical activity:
  • Structure-based inference
Annotation changelog
MIBiG version Submitter Notes
1.0
  • Hidden contributor (ID: RIBLHRV4YSJAGJ5EVZV4GS2Z, no GDPR consent given).
  • Submitted
2.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Migrated from v1.4
3.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Corrected NRP module activity
  • Corrected NRP substrate specificities
  • Updated NRP substrate specificities
3.1
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Update chemical activity to schema version 2.11
Detailed domain annotation
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