BGC0000398: orfamide B biosynthetic gene cluster from Pseudomonas sp. CMR12a
Shows the layout of the region, marking coding sequences and areas of interest. Clicking a gene will select it and show any relevant details. Clicking an area feature (e.g. a candidate cluster) will select all coding sequences within that area. Double clicking an area feature will zoom to that area. Multiple genes and area features can be selected by clicking them while holding the Ctrl key.
Location: 1 - 45,376 nt. (total: 45,376 nt).
This entry is originally from NCBI GenBank JQ309921.1.

Legend:

core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
reset zoomreset view
zoomzoom to selection
Gene details
Shows details of the most recently selected gene, including names, products, location, and other annotations.
Select a gene to view the details available for it
General
Compounds
Genes
NRP
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0000398
Short description orfamide B biosynthetic gene cluster from Pseudomonas sp. CMR12a
Status Minimal annotation: no
A minimal annotation only contains information on the BGC loci and one or more linked chemical product(s)

Completeness: complete
Whether the loci encodes everything needed for the pathway producing the compound(s)
Remarks "orfamide A and B possess a 3-OH-tetradecanoic acid lipid moiety, while orfamide C possesses 3-OH dodecanoic acid"
Biosynthetic class(es)
  • NRP (Cyclic depsipeptide)
Loci NCBI GenBank: JQ309921.1
Compounds
  • orfamide B
Species Pseudomonas sp. CMR12a [taxonomy]
References
Chemical products information
orfamide B
Copy SMILES
C63H112N10O17
Chemical database entries
NPAtlas
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • AFH75326.1
2082 - 2816 (+) putative acetyltransferase
copy AA seq
copy Nt seq
  • AFH75327.1
2951 - 3751 (-) LuxR family transcriptional regulator
  • Regulation
Knock-out
Sequence-based prediction
copy AA seq
copy Nt seq
  • AFH75328.1
  • ofaA
4357 - 10734 (+) nonribosomal peptide synthetase
  • Scaffold biosynthesis
Knock-out
Sequence-based prediction
copy AA seq
copy Nt seq
  • AFH75329.1
  • ofaB
11026 - 24123 (+) nonribosomal peptide synthetase
  • Scaffold biosynthesis
Knock-out
Sequence-based prediction
copy AA seq
copy Nt seq
  • AFH75330.1
  • ofaC
24120 - 38810 (+) nonribosomal peptide synthetase
  • Scaffold biosynthesis
Knock-out
Sequence-based prediction
copy AA seq
copy Nt seq
  • AFH75331.1
  • macA
39000 - 40121 (+) macrolide-specific efflux protein
  • Transport
Knock-out
Sequence-based prediction
copy AA seq
copy Nt seq
  • AFH75332.1
  • macB
40124 - 42082 (+) macrolide export ATP-binding/permease protein MacB
  • Transport
Knock-out
Sequence-based prediction
copy AA seq
copy Nt seq
  • AFH75333.1
42125 - 42895 (-) LuxR family transcriptional regulator
  • Regulation
Knock-out
Sequence-based prediction
copy AA seq
copy Nt seq
  • AFH75334.1
43363 - 43779 (-) glyoxalase family protein
copy AA seq
copy Nt seq
  • AFH75335.1
43969 - 45375 (-) heme biosynthesis protein
copy AA seq
copy Nt seq
NRP-specific information
Subclass Cyclic depsipeptide
Cyclic? yes
Thioesterase genes
  • ofaC (Unknown)
NRP-synthases
Gene Modules
ofaA
Module A1
Specificity: leucine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: Starter
Module A2
Specificity: glutamic acid
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
ofaB
Module B1
Specificity: threonine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: Dual
Module B2
Specificity: valine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: Dual
Module B3
Specificity: leucine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: Dual
Module B4
Specificity: serine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: Dual
ofaC
Module C3
Specificity: serine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: DCL
Module C2
Specificity: leucine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
Module C1
Specificity: leucine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: Dual
Module C4
Specificity: valine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: Dual
Annotation changelog
MIBiG version Submitter Notes
1.0
  • Hidden contributor (ID: 4VPAMSFU7CTQRXZA3KNXYTFK, no GDPR consent given).
  • Submitted
2.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Migrated from v1.4
  • Updated compound(s) information (NPAtlas curation)
3.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Corrected NRP module activity
  • Updated bioactivity data
  • Updated NRP substrate specificities
3.1
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Update chemical activity to schema version 2.11
Detailed domain annotation
Shows NRPS- and PKS-related domains for each feature that contains them. Click on each domain for more information about the domain's location, consensus monomer prediction, and other details.
A glossary is available here.
Selected features only
Show module domains
Similar known gene clusters
Shows clusters from the MiBIG database that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
Click on reference genes to show details of similarities to genes within the current region.
Click on an accession to open that entry in the MiBIG database.