BGC0000399: orfamide A biosynthetic gene cluster from Pseudomonas protegens Pf-5
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Location: 2,364,934 - 2,411,953 nt. (total: 47,020 nt).
This entry is originally from NCBI GenBank CP000076.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
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Gene details
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General
Compounds
Genes
NRP
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0000399
Short description orfamide A biosynthetic gene cluster from Pseudomonas protegens Pf-5
Status Minimal annotation: no
A minimal annotation only contains information on the BGC loci and one or more linked chemical product(s)

Completeness: complete
Whether the loci encodes everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • NRP (Cyclic depsipeptide)
Loci NCBI GenBank: CP000076.1
Compounds
  • orfamide A
  • orfamide C
Species Pseudomonas protegens Pf-5 [taxonomy]
References
Chemical products information
orfamide A
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C64H114N10O17
Chemical database entries
NPAtlas
orfamide C
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C62H110N10O17
Chemical database entries
NPAtlas
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • PFL_2142
  • AAY91416.1
 2364934 - 2366364 (-) efflux transporter, outer membrane factor lipoprotein, NodT family
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  • PFL_2143
  • AAY91417.1
 2366424 - 2367224 (-) transcriptional regulator, LuxR family
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  • PFL_2144
  • AAY91418.1
 2367557 - 2367703 (-) conserved hypothetical protein
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  • PFL_2145
  • AAY91419.3
  • ofaA
 2367819 - 2374184 (+) non-ribosomal peptide synthetase OfaA
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  • PFL_2146
  • AAY91420.2
  • ofaB
 2374502 - 2387605 (+) non-ribosomal peptide synthetase OfaB
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  • PFL_2147
  • AAY91421.3
  • ofaC
 2387602 - 2402307 (+) non-ribosomal peptide synthetase OfaC
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  • PFL_2148
  • AAY91422.1
 2402458 - 2403609 (+) efflux transporter, RND family, MFP subunit
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  • PFL_2149
  • AAY91423.1
 2403612 - 2405570 (+) efflux ABC transporter, permease/ATP-binding protein
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  • PFL_2150
  • AAY91424.1
 2405631 - 2406419 (-) transcriptional regulator, LuxR family
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  • PFL_2151
  • AAY91425.1
 2406647 - 2407060 (-) glyoxalase family protein
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  • PFL_2152
  • AAY91426.1
 2407236 - 2408744 (-) radical SAM domain protein
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  • PFL_2153
  • AAY91427.1
 2408835 - 2409443 (-) conserved hypothetical protein
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  • PFL_2154
  • AAY91428.1
 2409495 - 2410130 (-) conserved hypothetical protein
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  • PFL_2155
  • AAY91429.1
 2410167 - 2410355 (-) conserved hypothetical protein
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  • PFL_2156
  • AAY91430.1
 2410673 - 2411302 (-) HAD hydrolase, family IA, variant 3
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  • PFL_2157
  • AAY91431.1
 2411415 - 2411672 (+) conserved hypothetical protein
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  • PFL_2158
  • AAY91432.1
 2411768 - 2411953 (+) conserved hypothetical protein
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NRP-specific information
Subclass Cyclic depsipeptide
Cyclic? yes
Release type
  • Macrolactonization
NRP-synthases
Gene Modules
ofaA
Module 1
Specificity: leucine
Evidence for specificity:
  • Structure-based inference
  • Sequence-based prediction
Condensation domain type: Starter
Module 2
Specificity: glutamic acid
Evidence for specificity:
  • Structure-based inference
  • Sequence-based prediction
Condensation domain type: Dual
ofaB
Module 3
Specificity: threonine
Evidence for specificity:
  • Structure-based inference
  • Sequence-based prediction
Condensation domain type: Dual
Module 4
Specificity: isoleucine
Evidence for specificity:
  • Structure-based inference
  • Sequence-based prediction
Condensation domain type: Dual
Module 5
Specificity: leucine
Evidence for specificity:
  • Structure-based inference
  • Sequence-based prediction
Condensation domain type: Dual
Module 6
Specificity: serine
Evidence for specificity:
  • Structure-based inference
  • Sequence-based prediction
Condensation domain type: Dual
ofaC
Module 7
Specificity: leucine
Evidence for specificity:
  • Structure-based inference
  • Sequence-based prediction
Condensation domain type: Dual
Module 8
Specificity: leucine
Evidence for specificity:
  • Structure-based inference
  • Sequence-based prediction
Condensation domain type: LCL
Module 9
Specificity: serine
Evidence for specificity:
  • Structure-based inference
  • Sequence-based prediction
Condensation domain type: LCL
Module 10
Specificity: valine
Evidence for specificity:
  • Structure-based inference
  • Sequence-based prediction
Condensation domain type: Dual
Annotation changelog
MIBiG version Submitter Notes
1.0
  • Hidden contributor (ID: KRKW7VUNYGF36O3Z7FAQ6YXG, no GDPR consent given).
  • Submitted
2.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Migrated from v1.4
  • Updated compound(s) information (NPAtlas curation)
3.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Corrected NRP module activity
  • Corrected gene identifiers
  • Updated NRP substrate specificities
3.1
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Update chemical activity to schema version 2.11
Detailed domain annotation
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