BGC0000459: griseoviridin biosynthetic gene cluster from Streptomyces griseoviridis
Shows the layout of the region, marking coding sequences and areas of interest. Clicking a gene will select it and show any relevant details. Clicking an area feature (e.g. a candidate cluster) will select all coding sequences within that area. Double clicking an area feature will zoom to that area. Multiple genes and area features can be selected by clicking them while holding the Ctrl key.
Location: 13,530 - 118,200 nt. (total: 104,671 nt).
This entry is originally from NCBI GenBank JX508597.1.

Legend:

core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
reset zoomreset view
zoomzoom to selection
Gene details
Shows details of the most recently selected gene, including names, products, location, and other annotations.
Select a gene to view the details available for it
General
Compounds
Genes
Polyketide
NRP
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0000459
Short description griseoviridin biosynthetic gene cluster from Streptomyces griseoviridis
Status Minimal annotation: no
A minimal annotation only contains information on the BGC loci and one or more linked chemical product(s)

Completeness: complete
Whether the loci encodes everything needed for the pathway producing the compound(s)
Remarks "VG is synthesised by the NRPS within this cluster whereas GV is hybrid PK-NRP and as such utilises the PKS machinery also."
Biosynthetic class(es)
  • NRP (Cyclic depsipeptide)
  • Polyketide (Other)
Loci NCBI GenBank: JX508597.1
Compounds
  • griseoviridin
  • fijimycin A
Species Streptomyces griseoviridis [taxonomy]
References
Chemical products information
griseoviridin [synonyms: virginiamycin m, pristinamycin IIA]
Copy SMILES
C22H27N3O7S1
Chemical database entries
NPAtlas
fijimycin A
Copy SMILES
C44H62N8O11
Chemical database entries
NPAtlas
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • AGN74872.1
13530 - 14696 (+) SARP-type regulator
  • Regulation
Knock-out
copy AA seq
copy Nt seq
  • AGN74873.1
14816 - 16408 (-) major facilitator transporter
  • Transport
Sequence-based prediction
copy AA seq
copy Nt seq
  • AGN74874.1
16726 - 17847 (-) branched-chain amino acid aminotransferase
  • Precursor biosynthesis
Sequence-based prediction
copy AA seq
copy Nt seq
  • AGN74875.1
17916 - 18935 (-) methyltransferase
  • Precursor biosynthesis
Sequence-based prediction
copy AA seq
copy Nt seq
  • AGN74876.1
19073 - 26989 (-) nonribosomal peptide synthetase
  • Scaffold biosynthesis
Sequence-based prediction
copy AA seq
copy Nt seq
  • AGN74877.1
27311 - 28057 (+) type ii thioestrerase
  • Scaffold biosynthesis
Knock-out
copy AA seq
copy Nt seq
  • AGN74878.1
28052 - 29134 (-) flavin-dependent oxidoreductase
  • Precursor biosynthesis
Knock-out
copy AA seq
copy Nt seq
  • AGN74879.1
29368 - 29613 (-) hypothetical protein
  • Unknown
Sequence-based prediction
copy AA seq
copy Nt seq
  • AGN74880.1
29782 - 31404 (+) 3-hydroxypicolinic acid:AMP ligase
  • Scaffold biosynthesis
Knock-out
copy AA seq
copy Nt seq
  • AGN74881.1
31408 - 32709 (+) L-lysine 2-aminotransferase
  • Precursor biosynthesis
Sequence-based prediction
copy AA seq
copy Nt seq
  • AGN74882.1
32763 - 32951 (+) 4-oxalocrotonate tautomerase
  • Unknown
Sequence-based prediction
copy AA seq
copy Nt seq
  • AGN74883.1
33036 - 34343 (-) phenylacetyl-CoA dioxygenase
copy AA seq
copy Nt seq
  • AGN74884.1
34358 - 34609 (-) MbtH domain-containing protein
  • Scaffold biosynthesis
Knock-out
copy AA seq
copy Nt seq
  • AGN74885.1
34683 - 51854 (-) nonribosomal peptide synthetase
  • Scaffold biosynthesis
Sequence-based prediction
copy AA seq
copy Nt seq
  • AGN74886.1
51851 - 56554 (-) nonribosomal peptide synthetase
  • Scaffold biosynthesis
Sequence-based prediction
copy AA seq
copy Nt seq
  • AGN74887.1
57108 - 58265 (+) branched-chain alpha-keto acid dehydrogenase E1 subunit alpha
  • Precursor biosynthesis
Knock-out
copy AA seq
copy Nt seq
  • AGN74888.1
58262 - 59317 (+) branched-chain alpha-keto acid dehydrogenase E1 subnuit beta
  • Precursor biosynthesis
Knock-out
copy AA seq
copy Nt seq
  • AGN74889.1
59323 - 60720 (+) branched-chain alpha-keto acid dehydrogenase E2
  • Precursor biosynthesis
Knock-out
copy AA seq
copy Nt seq
  • AGN74890.1
60959 - 62614 (+) ABC transporter
  • Transport
Sequence-based prediction
copy AA seq
copy Nt seq
  • AGN74891.1
62773 - 63963 (-) P450-like monooxygenase
copy AA seq
copy Nt seq
  • AGN74892.1
64158 - 77825 (+) nonribosomal peptide synthetase/polyketide synthase hybrid protein
  • Scaffold biosynthesis
Sequence-based prediction
copy AA seq
copy Nt seq
  • AGN74893.1
77828 - 84568 (+) nonribosomal peptide synthetase/polyketide synthase hybrid protein
  • Scaffold biosynthesis
Sequence-based prediction
copy AA seq
copy Nt seq
  • AGN74894.1
84740 - 91246 (+) nonribosomal peptide synthetase/polyketide synthase hybrid protein
  • Scaffold biosynthesis
Sequence-based prediction
copy AA seq
copy Nt seq
  • AGN74895.1
91257 - 99092 (+) nonribosomal peptide synthetase/polyketide synthase hybrid protein
  • Scaffold biosynthesis
Sequence-based prediction
copy AA seq
copy Nt seq
  • AGN74896.1
99089 - 104953 (+) nonribosomal peptide synthetase/polyketide synthase hybrid protein
  • Scaffold biosynthesis
Knock-out
copy AA seq
copy Nt seq
  • AGN74897.1
104950 - 105900 (+) acyltransferase
  • Scaffold biosynthesis
Knock-out
copy AA seq
copy Nt seq
  • AGN74898.1
105894 - 108320 (+) nonribosomal peptide synthetase
  • Scaffold biosynthesis
Knock-out
copy AA seq
copy Nt seq
  • AGN74899.1
108570 - 109964 (+) major facilitator transporter
  • Transport
Sequence-based prediction
copy AA seq
copy Nt seq
  • AGN74900.1
109996 - 110889 (-) streptogramin B lyase
  • Resistance/immunity
Sequence-based prediction
copy AA seq
copy Nt seq
  • AGN74901.1
111137 - 112285 (-) sarcosine oxidase
copy AA seq
copy Nt seq
  • AGN74902.1
112395 - 113237 (-) SARP-type regulator
  • Regulation
Knock-out
copy AA seq
copy Nt seq
  • AGN74903.1
113627 - 114274 (-) TetR-type regulator
  • Regulation
Knock-out
copy AA seq
copy Nt seq
  • AGN74904.1
114756 - 115520 (+) ketoreductase
  • Regulation
Knock-out
copy AA seq
copy Nt seq
  • AGN74905.1
115710 - 116678 (+) AfsA-like gamma-butyrolactone biosynthetic protein
  • Regulation
Knock-out
copy AA seq
copy Nt seq
  • AGN74906.1
116703 - 117386 (-) gamma-butyrolactone receptor
  • Regulation
Knock-out
copy AA seq
copy Nt seq
  • AGN74907.1
117511 - 118200 (-) phosphatase
copy AA seq
copy Nt seq
Polyketide-specific information
Subclass Other
Starter unit Acetyl-CoA
Cyclic? yes
Release type
  • Macrolactonization
Polyketide-synthases
Genes Properties Modules
AGN74877.1
+
AGN74892.1
+
AGN74893.1
+
AGN74895.1
+
AGN74894.1
+
AGN74897.1
Synthase subclass: Trans-AT type I
Trans-acyltansferases: AGN74897.1
Thioesterases:
  • AGN74877.1 (Unknown)
Module B1
Genes: AGN74892.1
Core domains: Ketosynthase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: D-OH
Module B2
Genes: AGN74892.1
Core domains: Ketosynthase, Dehydratase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: D-OH
Module B4
Genes: AGN74893.1
Core domains: Ketosynthase, Dehydratase, Ketoreductase, Thiolation (ACP/PCP), Ketosynthase
KR-domain stereochemistry: D-OH
Module B5
Genes: AGN74894.1
Core domains: Ketosynthase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module B6
Genes: AGN74894.1
Core domains: Ketosynthase, Thiolation (ACP/PCP)
Module B8
Genes: AGN74895.1
Core domains: Ketosynthase, Thiolation (ACP/PCP)
Module 0
Genes: AGN74892.1
Core domains: Thiolation (ACP/PCP)
NRP-specific information
Subclass Cyclic depsipeptide
Cyclic? yes
Release type
  • Macrolactonization
NRP-synthases
Gene Modules
AGN74880.1
Module 0
Specificity: 3-hydroxypicolinic acid
Evidence for specificity:
  • Sequence-based prediction
AGN74876.1
Module 1
Specificity: threonine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
Module 2
Specificity: leucine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
AGN74886.1
Module A3
Specificity: 4R-hydroxyproline
Evidence for specificity:
  • Structure-based inference
Condensation domain type: DCL
AGN74885.1
Module 4
Specificity: glycine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: DCL
Modification domains: Methylation
Module 5
Specificity: 3-methylleucine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
Modification domains: Methylation
Module 6
Specificity: alanine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
Module A7
Specificity: phenylglycine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
Modification domains: Methylation
AGN74892.1
Module B3
Specificity: glycine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: Unknown
AGN74895.1
Module B7
Specificity: serine
Evidence for specificity:
  • Structure-based inference
Modification domains: Unknown
AGN74898.1
Module B9
Specificity: cysteine
Evidence for specificity:
  • Structure-based inference
AGN74896.1
Module B10 [inactive]
Condensation domain type: Unknown
Module 9
Condensation domain type: Unknown
Annotation changelog
MIBiG version Submitter Notes
1.0
  • Hidden contributor (ID: AADADFAEMFE2AOMR24EUYNFH, no GDPR consent given).
  • Submitted
2.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Migrated from v1.4
  • Updated compound(s) information (NPAtlas curation)
3.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Corrected NRP module activity
  • Removed ketoreductase stereochemistry annotation from modules without ketoreductases
  • Sorted modules by module number
  • Corrected gene identifiers
  • Updated bioactivity data
  • Updated NRP substrate specificities
3.1
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Update chemical activity to schema version 2.11
Detailed domain annotation
Shows NRPS- and PKS-related domains for each feature that contains them. Click on each domain for more information about the domain's location, consensus monomer prediction, and other details.
A glossary is available here.
Selected features only
Show module domains
Similar known gene clusters
Shows clusters from the MiBIG database that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
Click on reference genes to show details of similarities to genes within the current region.
Click on an accession to open that entry in the MiBIG database.