LOCUS       BGC0000488             11388 bp    DNA     linear   BCT 15-APR-2005
DEFINITION  Clostridium beijerinckii cfgR gene, cfgK gene, cfg02 gene, cfg01
            gene, cirA gene, cirB gene, cirC gene, cirD gene, cirE gene, cirG
            gene, cirH gene and cirI gene, genomic RNA, strain ATCC 25752.
ACCESSION   BGC0000488
VERSION     BGC0000488
KEYWORDS    .
SOURCE      Clostridium beijerinckii
  ORGANISM  Clostridium beijerinckii
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
            Clostridium.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-07 19:39:57
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..11388
                     /db_xref="taxon:1520"
                     /mol_type="mRNA"
                     /organism="Clostridium beijerinckii"
                     /strain="ATCC 25752"
                     /type_material="type strain of Clostridium beijerinckii"
     protocluster    1..11388
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[2239:2839]"
                     /cutoff="20000"
                     /detection_rule="(TIGR04223 or AgrB)"
                     /neighbourhood="10000"
                     /product="cyclic-lactone-autoinducer"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      2240..2839
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(TIGR04223 or AgrB)"
                     /neighbourhood="10000"
                     /product="cyclic-lactone-autoinducer"
                     /protocluster_number="1"
     cand_cluster    1..11388
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(TIGR04223 or AgrB)"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="neighbouring"
                     /product="cyclic-lactone-autoinducer"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /protoclusters="2"
                     /tool="antismash"
     subregion       1..11388
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000488"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..11388
                     /candidate_cluster_numbers="1"
                     /candidate_cluster_numbers="2"
                     /contig_edge="True"
                     /product="cyclic-lactone-autoinducer"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(TIGR04223 or AgrB)"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /subregion_numbers="1"
                     /tool="antismash"
     protocluster    1..9006
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[3787:4006]"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="2"
                     /tool="antismash"
     proto_core      3788..4006
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="2"
     cand_cluster    1..9006
                     /candidate_cluster_number="2"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="2"
                     /tool="antismash"
     gene            162..938
                     /gene="cfgR"
     CDS             162..938
                     /codon_start=1
                     /db_xref="GOA:Q7WYU5"
                     /db_xref="InterPro:IPR001789"
                     /db_xref="InterPro:IPR007492"
                     /db_xref="InterPro:IPR008246"
                     /db_xref="InterPro:IPR011006"
                     /db_xref="UniProtKB/TrEMBL:Q7WYU5"
                     /gene="cfgR"
                     /product="putative response regulator"
                     /protein_id="CAD97576.1"
                     /transl_table=11
                     /translation="MFSVIIVEDDFMQRDILKKMILSMYEFIEIYEADSENTALDIIFK
                     HDINMFLIDISLKESSGLDLAMKIRNIDKYQFSQIIFLTTHMEYITQAFKQTHCYDYII
                     KPYNQEDVQDMLNKLIHYERSRLNNSNDNFNEYNKDNDKKVSINLKNGIYAVIKTDDIV
                     FFEIKGRVCEINTVNGIYEANNTSLKKIMKLVDCEYIVQSHRAFAINKNYIRKVEKIST
                     KLSIIYFNNCLKTALLGYKFKDNIMSEIKAGEMILC"
     gene            932..2209
                     /gene="cfgK"
     CDS             932..2209
                     /codon_start=1
                     /db_xref="GOA:Q7WYU4"
                     /db_xref="InterPro:IPR003594"
                     /db_xref="UniProtKB/TrEMBL:Q7WYU4"
                     /gene="cfgK"
                     /product="putative histidine kinase"
                     /protein_id="CAD97577.1"
                     /transl_table=11
                     /translation="MLNDFMLSIMDTFSVIYLWIILTKKEKHIFSLLASVIIISILVTV
                     TDHLGLNFIFTYIIDIIVIKVIYKKNFKNIIFELLLSVLIIILIQLILFVVINKFVYDS
                     TIQDFAIESIILVCLVIFSRFNLVNKNFIAEKITNDILFYFVLTSIIYVMIIKFIWEYD
                     RKIIMDNLIIISVVLSILIISQVFTYLYVAKVVKEKETLKVSNEYNSIINEIVQEIKQR
                     QHDFINYKNTIRGIVQVVEEKDIKSVITNYMKDEETYDNKINELIYIDNAIIRSIIYRD
                     ICKAEKYNINFKYQIENNVLDNILGYHEISNLLNNMLNNAFDEVLKEECDKKNIEVRIY
                     NLEGTPHLIVKNQIVNPNNINLNEMFTRGYTTKNIKGTRGYGLYNVQKIVNSHKGCINF
                     YVEHETIIFDIYFNNSSGKSGSPIRV"
     gene            complement(2240..2839)
                     /gene="cfg02"
     CDS             complement(2240..2839)
                     /codon_start=1
                     /db_xref="GOA:Q7WYU3"
                     /db_xref="InterPro:IPR006741"
                     /db_xref="UniProtKB/Swiss-Prot:Q7WYU3"
                     /gene="cfg02"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyclic-lactone-autoinducer: AgrB"
                     /gene_kind="biosynthetic"
                     /product="putative ArgB homologue"
                     /protein_id="CAD97578.1"
                     /sec_met_domain="AgrB (E-value: 6.7e-35, bitscore: 109.8,
                     seeds: 181, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIKYLSTNISLYFQENNSCLSKKDVLKIQYTLEAILSDLSKFIII
                     FLVFLFIKEIPLFLFSFIILNSTRPLLGGIHCKTYYGCLTCSILYFMIILLFTRLFPEL
                     NTNFYIVFFILSLAITFIFAPCPNEKRPVKNKATLKILSLISLTFWIILFYLSPLQTRN
                     CILISIFLQIIQVIIINTKGVIFNAKNNKTFFNRTT"
     gene            complement(3090..3482)
                     /gene="cfg01"
     CDS             complement(3090..3482)
                     /codon_start=1
                     /db_xref="UniProtKB/TrEMBL:Q7WYU2"
                     /gene="cfg01"
                     /product="hypothetical protein"
                     /protein_id="CAD97579.1"
                     /transl_table=11
                     /translation="MESTQLKKLGAGIVTISAIQLIGAIFSVLGSLLLFSSKSEILNEM
                     QTSKQNIASNTQITITLILQVLLAITIILILLKKSIGIYSYFTVVTINILYSIISIGFQ
                     WTIIFSLILPVLMAIFIYKKKSLFTF"
     gene            3788..4006
                     /gene="cirA"
     CDS             3788..4006
                     /codon_start=1
                     /db_xref="InterPro:IPR009086"
                     /db_xref="UniProtKB/TrEMBL:Q8GB47"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /function="anti-bacterial peptide"
                     /gene="cirA"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IId"
                     /gene_kind="biosynthetic"
                     /product="circularin A precursor"
                     /protein_id="CAD97580.1"
                     /sec_met_domain="Bacteriocin_IId (E-value: 2.6e-31,
                     bitscore: 97.3, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFLVAGALGVQTAAATTIVNVILNAGTLVTVLGIIASIASGGAGT
                     LMTIGWATFKATVQKLAKQSMARAIAY"
     mat_peptide     3797..4003
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /gene="cirA"
                     /product="circularin A"
     gene            4107..5852
                     /gene="cirB"
     CDS             4107..5852
                     /codon_start=1
                     /db_xref="UniProtKB/TrEMBL:Q7WYU1"
                     /gene="cirB"
                     /product="CirB protein"
                     /protein_id="CAD97581.1"
                     /transl_table=11
                     /translation="MKNSFFYLIDMLKILLKLKIRTQYNSIFKILKIKSPTNVYWILLI
                     LKTIFSSIIIFAIYSHYYDSNYNSWWFIIISLIIICLNLEYTFDSYKKITKPSNIELYI
                     ISALSESEISTLFNLLEYCWFKLSILDKLMPIKILFIVIFGIKSVLWLVFLESIFILTT
                     LVINNLIKSSYDKSISYSIVVKFINYIFTNIFIFFLLYIFSSFLAIGLKIFRSNMANSS
                     SLNQDTLASIEMQLNDLWITPVIEKLDIAINLFNQIKSNINILLTGNGYIILMMLSIVN
                     LIILFIIIKFKYIKYYYKHNNIIPNIYLKYINIHKKINLFFNSNNKLVLKDLNILIKGD
                     NELKTNFFTVTLFSYELIIEVAILISINSFIENIYLYSMLIIFFNNILILNQANNIINS
                     LTRIFKYKYDLPNIDIYKMSQTSLKDLYESKVKLGRLFMLIPTFIFVIFNSYMLVCKLN
                     ISTYYIVSQILLLILSIANYYLAPIFSLYMSPYIISFSNNEFEESDSLKDEDKLYSKFY
                     NIPKMILIMPVSYLFFINSVVNIFKYDTWIYINIAYCFLTLALVLIFNRYFRNIVHKGV
                     EKFEF"
     gene            5833..6390
                     /gene="cirC"
     CDS             5833..6390
                     /codon_start=1
                     /db_xref="UniProtKB/TrEMBL:Q7WYU0"
                     /gene="cirC"
                     /product="CirC protein"
                     /protein_id="CAD97582.1"
                     /transl_table=11
                     /translation="MKNLSFSNKLKDIISPNKIFTSLISIKKQILISFSFYFIGIIVGM
                     FLSRNQEYTTNPQNLKFAYIFFNNYRLSILIIIIGLITVGIGSSIMLFTNGVILGDILI
                     GVFNKYSVWPILTGVAPHFIFEISALCIATSISYESIRFIRLLLDKNYTNKVHIGRILS
                     NFVTMTVLFFVAALIETNIAHI"
     gene            6390..7055
                     /gene="cirD"
     CDS             6390..7055
                     /codon_start=1
                     /db_xref="GOA:Q7WYT9"
                     /db_xref="InterPro:IPR003439"
                     /db_xref="InterPro:IPR003593"
                     /db_xref="UniProtKB/TrEMBL:Q7WYT9"
                     /gene="cirD"
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 139.7; E-value:
                     2.1e-42)"
                     /gene_kind="transport"
                     /product="ATP-binding protein"
                     /protein_id="CAD97583.1"
                     /transl_table=11
                     /translation="MVKGWNYMKPMLEITNLSFSYSEETEVLKNLDFTLNEKEILCIKG
                     PNGAGKTTFLKILCGLIRINSCNLKYMGQKNKLRNIKDDIAYVPSDPYLYTKLTGMENL
                     ELICDIWKEDKKKFMLTSMELANFFNLEEDLNTYVEDYSLGMKHKLYLIGMLSRNTKII
                     IMDEPLTALDIQSQNIAIKMFHEYVQLNKSIIFVSHINDLITRLATKELNLVNGRFYD"
     gene            7085..7234
                     /gene="cirE"
     CDS             7085..7234
                     /codon_start=1
                     /db_xref="UniProtKB/TrEMBL:Q7WYT8"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /gene="cirE"
                     /product="circularin immunity protein"
                     /protein_id="CAD97584.1"
                     /transl_table=11
                     /translation="MNKKKLLIYAILFLIYIILFLTYNNSIFRIILVVSLGFLSSIISK
                     LQIK"
     gene            7302..8729
                     /gene="cirG"
     CDS             7302..8729
                     /codon_start=1
                     /db_xref="GOA:Q7WYT7"
                     /db_xref="InterPro:IPR003997"
                     /db_xref="InterPro:IPR006143"
                     /db_xref="InterPro:IPR011053"
                     /db_xref="UniProtKB/TrEMBL:Q7WYT7"
                     /gene="cirG"
                     /gene_functions="transport (smcogs) SMCOG1029:RND family
                     efflux transporter MFP subunit (Score: 201.1; E-value:
                     4.2e-61)"
                     /gene_kind="transport"
                     /product="putative hylD family protein"
                     /protein_id="CAD97585.1"
                     /transl_table=11
                     /translation="MPKLEIEKDKFESQIKEGTRLKKILSKKVKIIICIVIVVAVAFLL
                     TKLQTSGVKTDTKYTVLSKGNIVNSINVSGVVKSGTSSNVYSTLDNKIKEVRVKVGDKV
                     KAGDILAVLDSGDLEKDIEQAEATTAANEANNKMELNAAKKEYENTLYLYNNNLNSEIK
                     NAEEAVNSAKLSLEDKENIYEYNKMLLESGEISKQELKQKKIDFENAKSEYDKAAVALE
                     NAKVKALQELDTAKNNYEKAQNAANNNSERIALEKQQSNLEKCQIKAPIDGTVTSVSAV
                     AGDPGSGNLFRVEDLDNIEINASIKEIDRASIKVGQKAEIRTDSTGDAVIYGEVVSISP
                     TANTSEIPINTEKSQLTNTSTNTSTEEGFEAKIKISEPNESIYVGMSTRANIILSEKSD
                     IYTISYDSIVENDDGKSLYIAEKSDNNDYIVKDVPISTGLESDSSVEISGEGISDGTII
                     IDDPSTCKVGDKIQISG"
     gene            8729..9475
                     /gene="cirH"
     CDS             8729..9475
                     /codon_start=1
                     /db_xref="GOA:Q7WYT6"
                     /db_xref="HSSP:Q58206"
                     /db_xref="InterPro:IPR003439"
                     /db_xref="InterPro:IPR003593"
                     /db_xref="UniProtKB/TrEMBL:Q7WYT6"
                     /gene="cirH"
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 207; E-value:
                     5.4e-63)"
                     /gene_kind="transport"
                     /product="ATP binding protein"
                     /protein_id="CAD97586.1"
                     /transl_table=11
                     /translation="MSLLNKNIIEMKNIVKSFYIGTPNQLDILKNIDITIKEGEFISIV
                     GASGSGKSTLMNIIGALDRQTSGNYILDNFNMNEVSDDGLSQIRNKKIGFVFQTFNLIP
                     RSTALKNVELPMLYAGVDRKERLERAESLLNLVGMEDRITHVPNELSGGQKQRVAIARA
                     LANDPSIILADEPTGALDSSTGRVVMDLFHKVHELEGKTIILITHNQELAEETERIITM
                     KDGKIVSEQFNRMYTKKFSNGEKACL"
     gene            9466..10734
                     /gene="cirI"
     CDS             9466..10734
                     /codon_start=1
                     /db_xref="GOA:Q7WYT5"
                     /db_xref="InterPro:IPR003838"
                     /db_xref="UniProtKB/TrEMBL:Q7WYT5"
                     /gene="cirI"
                     /product="putative ABC transporter permease"
                     /protein_id="CAD97587.1"
                     /transl_table=11
                     /translation="MFIKENILLAISSIKANKMRSMLTMLGIIIGISSVIGIVSIGSAI
                     TASVSNTLGKMGASNIGVSVMQKSDNSSGDGVSLSSKEPEDSDLLSLEQINSFREVFKG
                     QISTISISEEINQGRVKDGHLYANVSITGVNDGYRDTNNVKMVNGKFISDRDVKGTKKS
                     AVVSDKLVKNIFKKNINPVGKEIKVYIDDEVETYTIVGVYKYESSSLIGGSVVSEKDIS
                     TNLYIPITTSKIMKKNKNYSNFTIMPKSNVDSEKLVKDIEKHGEKLYKNNKHWTLYVLD
                     NKKSMESIAAVFKSISIGIAVIASIALLVGGIGVMNIMLVSVTERTREIGTRKALGARS
                     GHIKTQFIIESVIICTIGGTIGMILGVGMGVIACLVLKSPISISIPSIIISFTFSTVIG
                     VFFGYYPAKKAAQLDPIEALRYE"
ORIGIN
        1 tgtaagtatt ttataaaaag tatttgcaag ctttattttt aagggggagt atatgaaaca
       61 agaaaaataa aaaaactatt attttaagct gatttatgat tgtaataaac tataaagaag
      121 agatataccc agaagtggta tacaaagggg agatgaaaat tttgttttcg gttattatag
      181 tagaagatga ttttatgcaa agagatattt taaagaaaat gattttatcg atgtatgaat
      241 ttatagaaat ttatgaagct gacagcgaaa atacagcttt agatataatt tttaaacatg
      301 atattaatat gtttttaata gacataagtt taaaggaatc ttcaggattg gatcttgcaa
      361 tgaagattag aaacatagat aaatatcaat ttagtcaaat cattttttta acaactcata
      421 tggaatatat aacgcaagca tttaagcaaa cacattgtta tgactatata ataaaaccat
      481 ataatcaaga agatgtacaa gatatgttaa ataaacttat acactatgaa agaagccgat
      541 taaacaatag taatgataat tttaatgaat ataataagga caatgataaa aaggtttcta
      601 taaatttaaa aaatgggata tatgcggtga taaaaacgga tgacatagta ttttttgaga
      661 ttaaggggag agtttgtgaa ataaatactg ttaatggaat atatgaggct aacaatacaa
      721 gtttaaaaaa gattatgaaa ttagttgatt gtgagtatat agtacaaagt catagagcct
      781 ttgctataaa caaaaattat attcgtaagg tagaaaagat aagtactaag ctaagtatta
      841 tatattttaa caattgctta aaaacagctc ttttggggta taaatttaaa gataatataa
      901 tgtctgaaat taaagctggg gaaatgatat tatgttaaat gattttatgc taagtattat
      961 ggatacattc agtgtaattt acctgtggat aattttaact aaaaaagaaa agcatatatt
     1021 tagtttatta gcaagtgtaa tcattatttc gattttggtt acggtaactg atcatttagg
     1081 actaaatttc atatttacat atataataga cattattgta attaaggtta tatataagaa
     1141 aaattttaaa aatatcatat ttgaattgtt attatcagta cttataatta tacttataca
     1201 attaatatta tttgtagtta ttaataaatt tgtttatgat tctactatcc aagatttcgc
     1261 gatagaatct ataatacttg tgtgtttagt tattttctca agatttaatt tagttaataa
     1321 aaatttcatt gctgaaaaaa taactaacga tattttattt tattttgtat taacatcaat
     1381 tatatatgtc atgatcatta aatttatttg ggaatatgat agaaaaataa taatggataa
     1441 tttaattatt atatcagttg tattgagtat attaattata tctcaagttt tcacatactt
     1501 atatgttgct aaagtagtaa aagaaaaaga aacattaaaa gtttctaatg agtacaattc
     1561 tatcattaat gagatagttc aagaaataaa acaaagacaa catgatttta ttaattataa
     1621 aaatacaata agaggaatag tacaagttgt agaagaaaaa gatataaaat cagtgattac
     1681 taattatatg aaagatgaag agacgtatga taataaaata aatgaactaa tatatataga
     1741 taatgcaatt attagatcta taatatatag agatatatgt aaagcagaaa aatataatat
     1801 aaattttaaa tatcagatag aaaataatgt tttagataat atattaggtt accatgaaat
     1861 atctaattta ttaaataata tgctcaataa tgcctttgat gaagtattaa aagaagaatg
     1921 tgataagaaa aatattgaag taagaattta taatttggag ggaacacctc atttaattgt
     1981 taaaaaccaa atagtcaatc caaataacat taacttaaat gagatgttta caagaggtta
     2041 tactactaaa aatataaaag gtactagagg atatggcttg tacaatgtac aaaaaatagt
     2101 taacagccat aaaggatgta taaattttta tgtggagcat gaaacgatta tttttgatat
     2161 ttattttaat aattcttcag gaaaatctgg ttcgcctata agagtatgac aactcgatct
     2221 tgatagtaaa acaaacaaac taagtagtac ggttgaaaaa agttttatta ttttttgcat
     2281 tgaaaatcac tcctttcgta ttaataataa taacttgaat tatttgtaag aatatactta
     2341 taagtataca gttacgtgtt tgtaaaggtg ataggtaaaa aagtataatc caaaaagtaa
     2401 gcgatattaa agataatatt tttaatgtcg ctttattttt tactggtctt ttttcattag
     2461 ggcaaggagc aaaaataaaa gtaattgcaa gagatagtat aaagaaaact atataaaaat
     2521 tagtatttag ttctggaaat aatctagtaa ataataatat tatcatgaaa tatagtatgc
     2581 tgcaggttag acacccatag taagtcttgc agtgaattcc tcctaacaaa ggccttgttg
     2641 aatttaaaat tataaaagag aacagaaata atggaatttc ttttataaac aaaaaaacta
     2701 aaaatatgat aataaattta cttaaatcac ttaaaattgc ttctaaggta tattgaattt
     2761 taagtacatc ttttttgctt agacaagaat tattctcttg aaaatatagt gatatattag
     2821 tcgataaata tttaatcatc tttaacctct ttatgcaatt agtagtcaat attaaatatg
     2881 tgtatatata tatttatata tacaatattt aatggaaaat caatactaat cgcattaaaa
     2941 gcatagatat tgttattaaa agattatatt taataaagtg gaaataagtt ataagtaagt
     3001 gaaaaataaa aaaagcattt tcttatcttt aaagaaaata ctttttataa agctctagta
     3061 aatatatatt tatttcatag ctaattccac taaaaggtga agagactctt ttttttatat
     3121 ataaaaattg ccattaatac tgggagtatt aatgagaaaa tgattgtcca ttgaaaacca
     3181 atactaataa ttgaataaag tatattaatt gtaacaactg taaaataaga atatattcct
     3241 atagattttt taagcaaaat gagaataatt gttatagcaa gtaatacttg caatattaaa
     3301 gttatagtaa tttgagtatt agatgcgatg ttttgtttgc ttgtctgcat ttcatttaga
     3361 atttcacttt ttgatgagaa taacaataat gatcctaaaa cactaaatat agcaccgatc
     3421 aactgaatag ccgatattgt aactattcct gctcctaatt tttttaattg agtactttcc
     3481 ataataacct ccttgtatat gtaactaatt gtaaatattt agtttcttaa aaactcatgt
     3541 tatcataata tctaaatgaa atgaacattt ttagcattaa atgttcattt tggttcacaa
     3601 aaagagtatt ttgataaagt atgttaaaga ggttcctttt atgactaaat ttttacattt
     3661 aatgttataa gtatttactt tattatggtt atcatgtaaa aatgtaattg caagtgatta
     3721 aatagtttct tgcgattata ttgataacat atcaatatat cagtttttca aaaggaggtg
     3781 attaattatg tttttagttg caggagcact aggcgtgcaa acagctgcag ctactacaat
     3841 tgttaatgtt attcttaatg caggtacatt agttactgtt ttaggtatta tagcttcaat
     3901 tgcaagtggt ggtgcaggta cattaatgac tattggatgg gcaacattca aagcaacagt
     3961 tcaaaaatta gctaagcaaa gtatggcaag agctatagct tactaatcaa aatttatgat
     4021 tttaattaaa tggatagctt gctacagaca ggctatctat ttaattaaat gaataaaaaa
     4081 tcttgtattt attggagaaa ataaaaatga aaaatagctt tttttattta atagatatgc
     4141 taaaaatttt attaaagtta aaaattagaa ctcaatataa ttcaattttt aaaattttaa
     4201 aaattaaatc acctacaaat gtatattgga tcttacttat attaaaaact atttttagct
     4261 caattataat ttttgctata tattctcatt attacgactc aaactataac tcttggtggt
     4321 ttattataat atcacttatt ataatatgct taaatttaga atatacgttt gatagttata
     4381 aaaaaataac taaaccatca aatatagagc tatatataat aagtgcacta tctgaatcag
     4441 aaatatctac tttgtttaat ttactagaat attgctggtt taagcttagc atacttgata
     4501 agttaatgcc aataaaaata ctatttatag ttatatttgg aattaaatct gttctttggt
     4561 tagtgttttt ggaaagtatt tttattttaa ctacattagt tattaataat ttaattaaga
     4621 gttcgtatga taagtcaatc agctattcaa ttgtagtaaa atttataaat tatattttta
     4681 ctaacatatt tattttcttc ttattatata ttttttccag tttcttagca ataggattaa
     4741 aaattttccg aagtaatatg gcaaattcat ctagccttaa tcaagataca ttggcttcta
     4801 tagaaatgca gttaaatgat ttatggatta ctccggtaat tgaaaaatta gatattgcta
     4861 ttaacttatt taatcaaata aaatcaaata ttaatatact attaacagga aacggatata
     4921 ttattctaat gatgctttca atagttaatc tcattatact atttataata ataaaattta
     4981 aatatataaa atattattac aaacacaata atattattcc caatatatat ttaaaatata
     5041 taaatattca taaaaaaata aacttatttt ttaatagtaa taataaatta gtattaaaag
     5101 atttgaacat attgattaaa ggagataacg aattaaaaac taattttttt acagttacat
     5161 tattctcata tgaacttatt attgaagtag ctattcttat ttcaataaat agttttatag
     5221 aaaatatata tttatattca atgcttataa tattttttaa taatatttta atattaaatc
     5281 aagctaataa cataataaat tcactaacta gaatctttaa gtataaatat gacctaccta
     5341 atatagatat atataaaatg tctcaaactt ctctaaaaga tttatatgaa tcaaaagtta
     5401 aattgggaag attatttatg ttgattccaa catttatttt tgtaattttt aatagctaca
     5461 tgttagtatg taaacttaat attagcactt attatattgt atcccaaata ctactattaa
     5521 tactatctat tgcaaattat tatttggctc caatattctc attatatatg agtccgtata
     5581 taataagctt ttcaaataat gaatttgagg aaagcgatag tttaaaagat gaagataaat
     5641 tatattctaa attttataat attcctaaaa tgatactaat tatgcctgta tcatacctat
     5701 tttttataaa tagtgtagtt aatattttta aatatgatac ttggatttat attaatatag
     5761 cttattgttt tttgacttta gctttagttt taatatttaa cagatatttt agaaatatag
     5821 tccataaagg agttgaaaaa tttgagtttt agtaataagt tgaaagatat aatttcccca
     5881 aacaaaattt tcacctcatt aatatcaata aaaaagcaaa ttttaatttc attttcattt
     5941 tattttatag gtattattgt tggaatgttt ctttctagga atcaggaata tactactaac
     6001 ccacaaaacc tgaagtttgc atatattttc tttaataact acaggttatc gatcttaatt
     6061 ataattatag gattgataac agttggaata gggagctcaa taatgttatt taccaacgga
     6121 gttattctag gagatatatt aataggggtt tttaataaat atagtgtttg gccaatttta
     6181 acaggagtgg ctccacattt tatctttgag atctcagcat tatgcattgc tacatctatt
     6241 tcttatgaaa gtatccgatt tattcgtctt ttacttgata agaattatac taataaagtg
     6301 cacataggta ggattttaag taattttgtc acaatgacag ttttattctt tgttgctgct
     6361 ttaattgaaa ctaatatagc acatatttaa tggtgaaagg atggaattat atgaagccta
     6421 tgctagagat aactaattta agtttttcat atagtgaaga aactgaagta ttgaaaaact
     6481 tagatttcac tttgaatgaa aaagaaattt tatgtattaa aggaccaaat ggtgctggaa
     6541 agactacatt ccttaaaata ttatgtggct taattcggat taatagttgt aacttaaaat
     6601 atatggggca aaaaaataag cttagaaata taaaagatga tatcgcatat gttcctagtg
     6661 atccatattt atatacaaag cttactggaa tggagaattt ggaacttatt tgcgatatat
     6721 ggaaggaaga taaaaaaaaa tttatgctaa catccatgga attagcaaat ttttttaatt
     6781 tagaagaaga tctaaataca tatgtagaag attattctct tgggatgaaa cacaagttat
     6841 acctaatagg tatgttatct agaaatacta aaattattat tatggatgaa cctttaacag
     6901 cattagatat tcaaagtcaa aatattgcca taaaaatgtt ccacgagtat gttcaattaa
     6961 ataaatcaat aatttttgta agtcatataa atgatttaat aactagatta gcaacaaaag
     7021 aacttaatct agttaacgga agattttatg attaaactaa tttattagaa aggtagtaga
     7081 atatatgaat aaaaaaaaat tattaatata cgcaattctt tttttgatat acataattct
     7141 atttttaaca tataacaata gtatttttag aattatatta gttgtatcct taggtttttt
     7201 atctagtata atatctaaat tacaaattaa atagaatgat gtaattaaag gctctaataa
     7261 gtttgagcaa taactaatgt taaagtggga gaggttttaa aatgccaaag ttagagattg
     7321 aaaaggataa atttgagagc caaataaaag aaggcacaag attaaaaaag atactaagta
     7381 aaaaagtaaa gataataata tgtatagtta ttgtagtagc tgtcgctttt ttattgacta
     7441 aattacaaac gtctggagta aaaacagata ctaagtatac agttctctca aaaggaaata
     7501 tagttaatag tattaatgtt tctggtgtag taaaaagtgg tacatcatct aatgtatata
     7561 gtacgttaga taataaaata aaagaagtta gggtgaaagt tggggataaa gttaaagcag
     7621 gagatatttt agcagtatta gactcaggag atttagagaa ggatattgaa caagcggaag
     7681 caactacagc agccaatgaa gctaataata aaatggaact taatgctgca aaaaaagaat
     7741 atgagaatac actatattta tataataata atttgaattc agagataaaa aatgcggaag
     7801 aagctgtaaa ttcagcaaaa cttagtttgg aagataaaga aaatatttat gaatataata
     7861 aaatgttatt agaatctgga gaaatctcta agcaggaatt aaagcaaaaa aagatagatt
     7921 ttgaaaatgc aaagtcagaa tatgataagg ctgcagttgc acttgagaat gcgaaggtta
     7981 aagctttgca agaattagat acagcaaaaa acaactatga aaaagcccaa aacgctgcta
     8041 ataacaatag cgaaaggatt gctcttgaaa aacaacaaag caatcttgaa aagtgtcaaa
     8101 ttaaagcacc tattgatgga acggttacat cagtaagcgc agttgcagga gatcctggat
     8161 ctggaaactt attcagagtt gaggatttgg ataatattga aataaatgca tccataaagg
     8221 agattgacag agcaagtatt aaagttgggc aaaaagcaga gataagaaca gattcaacag
     8281 gggatgctgt tatttacgga gaagttgtaa gtattagtcc aacagctaat acgtcagaaa
     8341 tacctataaa tacagaaaag tctcaattga caaatacaag tacaaataca agcacagagg
     8401 aaggttttga agcaaaaatt aagattagtg agccaaatga gagtatttat gttggaatga
     8461 gtacaagagc taatattatt ttaagtgaaa aatcagatat atatactatt tcatatgaca
     8521 gtatagttga aaatgatgat ggtaagagtt tatatatagc agagaaaagt gataataatg
     8581 attatatagt taaggacgtg ccaataagta caggacttga atctgattca agtgtagaaa
     8641 tttcaggcga aggaatttca gatggaacca taataattga tgatccttca acttgcaagg
     8701 ttggggataa aattcaaata agtgggtgat gagtcttttg aataaaaata taatagagat
     8761 gaaaaatatt gtcaaaagct tttatatagg aactccaaat caattagata ttttaaaaaa
     8821 cattgatata acaataaaag aaggcgaatt tatatctata gttggagcat caggatctgg
     8881 taaaagtact ttgatgaaca tcattggtgc acttgatagg cagacatcag gaaactatat
     8941 cttagataat ttcaatatga atgaagtatc tgatgatgga ttatcacaaa tacgcaataa
     9001 gaagatagga tttgtatttc aaacttttaa tttaatacca agaagtacag cattaaaaaa
     9061 tgtagaatta ccaatgcttt atgcaggagt agatagaaaa gaacgcttag agagagccga
     9121 aagtcttttg aaccttgttg gaatggaaga taggataacc catgttccaa atgagttatc
     9181 aggcgggcaa aaacaaagag ttgcaattgc ccgtgctctt gcaaatgatc caagtataat
     9241 cttagcagat gaacctacag gagctcttga ttcttcaact ggaagagtgg ttatggacct
     9301 atttcacaaa gtacatgaat tggaaggtaa gactataata cttataactc ataatcagga
     9361 gcttgctgaa gaaacagaac gaattattac aatgaaggat gggaaaatag tatcagaaca
     9421 atttaataga atgtatacta aaaaattctc aaatggtgaa aaggcatgtt tataaaagaa
     9481 aatatactac ttgcaatatc atcaataaag gcaaataaaa tgcgctcgat gttgactatg
     9541 cttggaatta taatcgggat atcttctgtt atagggattg tttcaatcgg aagtgcaatc
     9601 accgcttcag tatcaaatac attaggtaaa atgggagcta gcaatattgg agttagtgta
     9661 atgcaaaaaa gtgataatag ttctggtgac ggagttagtt tatctagtaa agagcctgaa
     9721 gatagtgacc ttctttcttt ggaacaaata aattcattta gagaagtatt taaaggtcaa
     9781 attagcacta taagtatatc agaagaaata aatcaaggaa gagtaaagga tggacattta
     9841 tatgcaaatg taagtattac tggagtaaat gatggatata gagatactaa taacgtaaag
     9901 atggttaatg gaaaatttat ttccgataga gatgttaaag gaacgaaaaa aagtgctgta
     9961 gtttctgata aacttgttaa gaatattttt aaaaaaaata ttaatccagt tggtaaagaa
    10021 attaaagttt atatagatga tgaggtcgaa acatatacta ttgtgggagt atataaatat
    10081 gagagttctt cattgattgg aggaagtgta gtatcagaaa aagatatatc aaccaattta
    10141 tacattccta taacaacatc taaaataatg aaaaagaata aaaattattc gaattttact
    10201 atcatgccta agtctaatgt tgattcagaa aaattagtga aagatataga aaaacatgga
    10261 gaaaagttgt ataagaataa taaacattgg acactttatg ttttagataa caaaaaaagt
    10321 atggagtcta tagccgctgt atttaagagt atatcaattg gaattgctgt aattgcatct
    10381 atagcattac ttgttggagg tataggggta atgaacataa tgcttgtatc tgtcactgaa
    10441 agaacaagag aaataggaac aaggaaagct ctcggtgcaa gaagtggtca tataaaaaca
    10501 caatttataa ttgagtcagt cataatatgt acaattggag gaacaatagg aatgatactt
    10561 ggggttggta tgggggttat tgcttgttta gtattaaaat caccaatatc aatatctata
    10621 ccatcaatca taataagttt cacattctca acggtaatag gagtattctt tggatattat
    10681 ccggctaaaa aagcagcaca gcttgaccct atagaagcgt taagatatga gtaataaaat
    10741 aaataacatt aagtagtaaa gataacactg acttataatg attttaagag tgttatcttt
    10801 aaatatatcc attaaacgtt agcatagcta ataataaatt aattaatata taataagtgt
    10861 agtgtaaata atcgcattat attataaatt aaggagtaag aaaatgaaaa aggtatttac
    10921 gaattttttt attatgacgt taatattaac aaacttgtct tttgcagaca taaatagtgt
    10981 acaagcagct actactaata aaacaatcaa agtttcatta gaaaatatta aagatattat
    11041 tatagagaat agttcagaga ttaaaattca acataataac atacaaataa aaaaagaata
    11101 ttataataac gctaaagata ttaaagataa aaatgagagt gcaaaagacg aggcagagaa
    11161 taaattaaag aatctaatgg gggattctaa tgccactaag gaacagataa agtatgccga
    11221 agatgcttta gaggctgcca aaaaagaagt caaaaatgat aatgatgcat tagtgcaggc
    11281 taggaaagca tataaagatg caaaaacagt ttatgaccaa aaggttgaaa ccgaggtcga
    11341 tgaagcgcaa aaagcttata tagattattt atctaatata tcaaatac
//
