Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0000529.1:
Cluster 1RiPP121407

BGC0000529, microbisporicin biosynthetic gene cluster from Microbispora corallina. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: microbisporicin A2
SMILES string: Copy to clipboard
Molecular formula: C94 H127 Cl N26 O26 S5
Exact molecular mass: 2232.95 Da (none)
Molecular activity: Antibacterial
Molecular target: Lipid II

Class-specific details

Biosynthetic class(es):
RiPP
RiPP subclass:
Lantipeptide (linear)
Peptides in this cluster:
ADK32554
RiPP core peptide sequence(s): VTSWSLCTPGCTSPGGGSNCSFCC
Leader peptide length: 33
Follower peptide length: -1
Recognition motif in leader peptide for modification: LDLD
Peptidase(s) involved in precursor cleavage:
Crosslinks in final product:
3=7 AA (Thioether)
8=11 AA (Thioether)
13=20 AA (Thioether)
18=23 AA (Thioether)
21=23 AA (Thioether)

Gene cluster description

microbisporicin (BGC0000529). Gene Cluster 1. Biosynthetic class = RiPP. GenBank HM536998, positions 1656-23062. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Detailed annotation

mibA leader / core peptide, putative Class I
MPADILETRTSETEDLLDLDLSIGVEEITAGPA - VDhbDhaWDhaLCDhbPGCDhbDhaPGGGDhaNCDhaFCC

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Homologous known gene clusters

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Knock-out studies, Heterologous expression, Sequence-based prediction
MIxS-compliance:Unknown
Contact for this cluster:Margherita Sosio and Lorena T. Fernandez-Martinez (Naicons Srl and John Innes Centre; Norwich Research Park; Norwich; NR4 7UH; United Kingdom)

Literature references

1. Foulston LC, Bibb MJ. (2010) Microbisporicin gene cluster reveals unusual features of lantibiotic biosynthesis in actinomycetes. Proc Natl Acad Sci U S A 107(30):13461-6. doi:
2. Sosio M et al. (2014 TI) Draft Genome Sequence of the Microbispora sp. Strain ATCC-PTA-5024, Producing the Lantibiotic NAI-107. LID - 10.1128/genomeA.01198-13 [doi] LID - e01198-13 [pii] Genome Announc 2(1). pii: e01198-13. doi: 10.1128/genomeA.01198-13.
3. Maffioli SI et al. (2014) Characterization of the congeners in the lantibiotic NAI-107 complex. J Nat Prod 77(1):79-84. doi: 10.1021/np400702t. Epub 2014 Jan 14.
4. Foulston L, Bibb M. (2011) Feed-forward regulation of microbisporicin biosynthesis in Microbispora corallina. J Bacteriol 193(12):3064-71. doi: 10.1128/JB.00250-11. Epub 2011 Apr 8.
5. Munch D et al. (2014) The lantibiotic NAI-107 binds to bactoprenol-bound cell wall precursors and impairs membrane functions. J Biol Chem 289(17):12063-76. doi: 10.1074/jbc.M113.537449. Epub