LOCUS       BGC0000559             14616 bp    DNA     linear   BCT 19-DEC-1997
DEFINITION  Bacillus subtilis spaB, spaT, spaC, subtilin (spaS), spaI, spaF,
            spaE, spaG, spaR spaK and YvaN genes, complete cds.
ACCESSION   BGC0000559
VERSION     BGC0000559
KEYWORDS    .
SOURCE      Bacillus subtilis subsp. spizizenii ATCC 6633 = JCM 2499
  ORGANISM  Bacillus subtilis subsp. spizizenii ATCC 6633 = JCM 2499
            Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
COMMENT     On Dec 19, 1997 this sequence version replaced gi:595315.
            ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-07 19:52:05
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..14616
                     /db_xref="ATCC:6633"
                     /db_xref="taxon:703612"
                     /mol_type="genomic DNA"
                     /organism="Bacillus subtilis subsp. spizizenii ATCC 6633 =
                     JCM 2499"
                     /strain="ATCC 6633"
                     /sub_species="spizizenii"
     protocluster    1..14616
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[494:6720]"
                     /cutoff="20000"
                     /detection_rule="((LANC_like and (Lant_dehydr_N or
                     Lant_dehydr_C)) and not (YcaO or TIGR03604))"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-i"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      495..6720
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="((LANC_like and (Lant_dehydr_N or
                     Lant_dehydr_C)) and not (YcaO or TIGR03604))"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-i"
                     /protocluster_number="1"
     cand_cluster    1..14616
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((LANC_like and (Lant_dehydr_N or
                     Lant_dehydr_C)) and not (YcaO or TIGR03604))"
                     /kind="single"
                     /product="lanthipeptide-class-i"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..14616
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000559"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..14616
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lanthipeptide-class-i"
                     /region_number="1"
                     /rules="((LANC_like and (Lant_dehydr_N or Lant_dehydr_C))
                     and not (YcaO or TIGR03604))"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            495..3587
                     /gene="spaB"
     CDS             495..3587
                     /codon_start=1
                     /gene="spaB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_N"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_C"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Lanthipeptide_LanB_RRE"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1155:Lantibiotic dehydratase domain protein (Score:
                     869.1; E-value: 6e-263)"
                     /gene_kind="biosynthetic"
                     /product="SpaB"
                     /protein_id="AAB91586.1"
                     /sec_met_domain="Lant_dehydr_N (E-value: 1.3e-129,
                     bitscore: 422.8, seeds: 73, tool: rule-based-clusters)"
                     /sec_met_domain="Lant_dehydr_C (E-value: 5.8e-33, bitscore:
                     104.5, seeds: 35, tool: rule-based-clusters)"
                     /sec_met_domain="Lanthipeptide_LanB_RRE (E-value: 1.6e-15,
                     bitscore: 47.5, seeds: 20, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKSLYTPTDYYMIRVPLVHQDLKNENSQDIDQLLHDLCNDSLFRE
                     QILVSSRTLYETIHTFLQAPDKLKGKKKRNFQQAILKYATRRATRTTPFGLFSSVGIGS
                     FSDKNHLSFNQHSFYKKARVDLEWLYQLIRKLENEYTDRLSFTLNSACYIKGDRAYLLY
                     STDGKSEEVSVRATSVFYLINELCGESAAYQDIIRCLIDNYPNTPINKINQYVADLIDK
                     EFLISNLRPPMTVSDQFQYLIDQAESRHIPNELIQACKDIQYQIDAYNRITIGEGEHQY
                     LNLIETMNKLIKASSPLQVDAGLADSSIQLDNETSLAISELASMFTYMASPSANTLDHL
                     EKYHNVFLERYGYEREVPLLEMLCSSTGIGAPATYTNPANEFFEETSFGEQFSPEMKQF
                     FMRKYFESVRKKAPIQLDDETFHRICNSEIADEEIPLSFELNFFVKLRNGRVKLYLGPN
                     VGSTRAGKTFGRFSHMSDSISEIIKTLHNKEKELTECNTKVCELSIVPNQTRSGNVTRN
                     VSYREKEMSLFTNSALHLNDSVKAEDILIGINKDHNFYARHKTTGEILSFESNHMFNPL
                     LMTNAVRFLLEISRDGKRKWNDFPWFSIYSDFKYIPEIKYKEITLSCEQWLIYKNDLSM
                     HSNASLEEIKSPFFEFHRTYELPQTFYIVNADNRLLIDIENDCTLDVFFWELKKTNHNQ
                     PLQLVAVEHDADALMDRNQNDYSGEIVVPLLRKQPEKPLYLPVLNAIEGSGSDRIKMPF
                     EDWLFIKLYCKQTREEELIAFEIADFYNQISDQYPVRHFFMRYRDPKPHIRLRFNGKAE
                     VLYSLFPQLLNWLKSLREKGLVSESVITQYEREIERYGGLSLMEAAEQLFCEDSKVVEM
                     MIRMHRMKDITISKEIAGMVSVIQFLEQFELTFEEQLTFLERNSLQNEYRTEFKKDREM
                     YIEICNSDRDWDNLKKTSDGGMLYETLKTRKMAAAHYAFLIKKAFDNKDEVYSRIGSII
                     HLHCNRLFGTDRELENKILTLCRHSLYAQRYQKMNGSLAWK"
     gene            3578..5422
                     /gene="spaT"
     CDS             3578..5422
                     /codon_start=1
                     /gene="spaT"
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 167; E-value:
                     9.5e-51)"
                     /gene_kind="transport"
                     /product="SpaT"
                     /protein_id="AAB91587.1"
                     /transl_table=11
                     /translation="MEVKEQLKLKELLFIMKQMPKTFKLIFTLERSLFLKLIRFSIITG
                     ILPIVSLYISQELINSLVTIRKEVSIVITIFLTYLGVSFFSELISQISEFYNGKFQLNI
                     GYKLNYKVMKKSSNLALKDFENPEIYDKLERVTKEISYKPYQIIQAIITMTTSFVTLLS
                     SIAFLMSWNPKVSLLLLVIPVISLFYFLKIGQEEFFIHWKRAGKERKSWYISYILTHDF
                     SFKELKLYNLKDYLLNKYWDIKKSFIEQDTKILRKKTLLNLIYEIAVQLVGAVIIFIAI
                     MSAFAGKIMVGNVMSYIRSVSLVQNHSQSIMTSIYSIYNSNLYMNQLYEFLELKEEKSQ
                     GHKKPIVEPIHSVVFQNVSFIYPNQGEQTLKHINVSLHKGERVAIVGPNGSGKKTFIKL
                     LTGLYEVHEGDILINGINIKELDMDSYMNQIAALFQDFMKYEMTLKENIGFGQIDKLHQ
                     TNKMHEVLDIVRADFLKSHSSYQFDTQLGLWFDEGRQLSGGQWQKIALARAYFREASLY
                     ILDEPSSALDPIAEKETFDTFFSLSKDKIGIFISHRLVAAKLADRIIVMDKGEIVGIGT
                     HEELLKTCPLYKKMDESENYMNPLEEEGSKWKEALYQG"
     gene            5395..6720
                     /gene="spaC"
     CDS             5395..6720
                     /codon_start=1
                     /gene="spaC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: LANC_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1140:Lanthionine synthetase C family protein (Score:
                     412.9; E-value: 2.3e-125)"
                     /gene_kind="biosynthetic"
                     /product="SpaC"
                     /protein_id="AAB91588.1"
                     /sec_met_domain="LANC_like (E-value: 7.1e-91, bitscore:
                     294.3, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MERGTVSRIEVEIVKEMARQISNYDKVLEIVNQKDNFRSIGEVPL
                     IPWKSTALSHGIPGICMLYGELHAHFPEEGWDDIGHQYLSILVNEIKEKGLHTPSMFSG
                     AAGIGLAAICLSQRFTYYNGLISDINEYLAETVPQLLTEFDQRQVCMSDYDVIEGVSGI
                     ANYLLLFQEDKAMGDLLIDILKYLVRLTEDIIVDGEKVPGWHIPSQHQFTDIEKKAYPY
                     GNFNMGLAHGIPGPICVLSSALIQGIKVKGQEAAIEKMANFLLEFSEKEQDSLFWKGII
                     SFEEYQYGSPPNAVNFSRDAWCYGRPGVCLALVKAGKALQNTELINIGVQNLRYTISDI
                     RGIFSPTICHGYSGIGQILLAVNLLTGQEYFKEELQEIKQKIMSYYDKDYIFGFHNYES
                     MEGEEAVPLQYVGLLDGAVGVGLGVLNMELGSKTDWTKALLI"
     gene            6795..6965
                     /gene="spaS"
     CDS             6795..6965
                     /codon_start=1
                     /function="lantibiotic"
                     /gene="spaS"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TIGR03731"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) mature_a"
                     /gene_functions="biosynthetic-additional (lanthipeptides)
                     predicted lanthipeptide"
                     /gene_kind="biosynthetic-additional"
                     /note="subtilin"
                     /product="SpaS"
                     /protein_id="AAB91589.1"
                     /sec_met_domain="TIGR03731 (E-value: 1e-24, bitscore: 76.2,
                     seeds: 23, tool: rule-based-clusters)"
                     /sec_met_domain="mature_a (E-value: 2.9e-08, bitscore:
                     22.8, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSKFDDFDLDVVKVSKQDSKITPQWKSESLCTPGCVTGALQTCFL
                     QTLTCNCKISK"
     CDS_motif       6795..6866
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence: MSKFDDFDLDVVKVSKQDSKITPQ"
                     /locus_tag="spaS_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       6867..6965
                     /prepeptide="core"
                     /locus_tag="spaS_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class I"
                     /score="27.80"
                     /molecular_weight="3320.9"
                     /monoisotopic_mass="3318.6"
                     /core_sequence="WKSESLCTPGCVTGALQTCFLQTLTCNCKISK"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MSKFDDFDLDVVKVSKQDSKITPQ"
                     /leader_location="[6794:6866](+)"
                     /alternative_weights="3338.9"
                     /alternative_weights="3357.0"
                     /alternative_weights="3375.0"
     gene            7548..8045
                     /gene="spai"
     CDS             7548..8045
                     /codon_start=1
                     /function="immunity"
                     /gene="spai"
                     /product="SpaI"
                     /protein_id="AAB91598.1"
                     /transl_table=11
                     /translation="MLFLKRSVNILGCFIVLFMLSACQSLTKFKDKVVEETKKTMHFTD
                     DNENDTSETMESLIDKGKLDQVVYDDQLYHLKEKVDEDKKGKVIGAIGQTFFVDGDGKR
                     WSEEELKEPYISNNPDEIREKKPLRYGKVYSTNEDSDAKDEIIVEFNREYYRAVLIKNE
                     KE"
     gene            8032..8775
                     /gene="spaF"
     CDS             8032..8775
                     /codon_start=1
                     /function="immunity"
                     /gene="spaF"
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 182.2; E-value:
                     2.1e-55)"
                     /gene_kind="transport"
                     /product="SpaF"
                     /protein_id="AAB91597.1"
                     /transl_table=11
                     /translation="MKRNKGECDMSTVLLETKSLTKKFGKETAVADVSLTVQKNSIYGL
                     LGPNGAGKSTTLKILTGILRKTSGEILFDGHKWKRSDLQTIGSLIEAPPLYDNLTAFEN
                     VKVHATLLGLSEDRIHSVLKTVDMQHAGKKRAGQFSMGMRQRLGIAIALLNHPKLLILD
                     EPTNGLDPIGIQELRDLIRSFPQEGITVILSSHILSEVEQIADHIGIIIDGHLGYQGEI
                     NKADDLEHLFMEVVKSTREKEGVKR"
     gene            8772..9527
                     /gene="spaE"
     CDS             8772..9527
                     /codon_start=1
                     /function="immunity"
                     /gene="spaE"
                     /product="SpaE"
                     /protein_id="AAB91596.1"
                     /transl_table=11
                     /translation="MINYIKAENLKFKRTFSRKMIIFVALLNIGFSFLMNTQFFVPGTY
                     NWWSIIFMPVMIALFCSLSHQKEKKASHYNGTYSLPIDLGKLWYAKIIIIAIYSLLSQV
                     VFLVFMLLMGFVIADFSIISPSIVAASLLLWLTSLWQIPLCLFVAKKWGFTMAVMLNFI
                     GTLVLGVMPASRAFWWFIPWSWPIRMMCPTIGIHPNGLLLENNDPLLSWMVVPPAIVIS
                     ILFFLILAFLTSRSFAPTTDYKMNKREAM"
     gene            9688..10299
                     /gene="spaG"
     CDS             9688..10299
                     /codon_start=1
                     /function="immunity"
                     /gene="spaG"
                     /product="SpaG"
                     /protein_id="AAB91595.1"
                     /transl_table=11
                     /translation="MEAIAVALPLLIGVLCGMTASLEEQAGKFHVLLGSTSPKIVAYVS
                     KVFMLLFLEICSIGLALFIYFIGLKFILKVPDLSYDIFIMGGAWLVAGSVVLYCIYFFI
                     SCMFGMGASVLIGGAGLLMTALMNTGLGDAIWKFNPWAWGIRLSSLNEVLHFKEVNQGH
                     HPLLMHEINSGVLMMVFGIICTIIASLIWFSKWEGRKTFE"
     gene            10317..10979
                     /gene="spaR"
     CDS             10317..10979
                     /codon_start=1
                     /function="response regulator"
                     /gene="spaR"
                     /gene_functions="regulatory (smcogs) SMCOG1008:response
                     regulator (Score: 168.8; E-value: 2.2e-51)"
                     /gene_kind="regulatory"
                     /product="SpaR"
                     /protein_id="AAB91594.1"
                     /transl_table=11
                     /translation="MAKILAVDDEKDILVLIQNILRRDQHQVDILDHVHGQPPDVFQGY
                     DLILLDVMMPDIDGFELCKQIRPLVDCPILFLTAKTEEEAIVKGLITGGDDYITKPFGV
                     RELSARVNAHLRRERRDKHQSKRVISGFLFHFDSKEVFINNNKLNLTKNEYKICEFLAQ
                     HKGRTFSREQIYEEIYGLEGNALYSTITEFIRTIRKKCKEHNADPIKTVWGVGYKWE"
     gene            10970..12349
                     /gene="spaK"
     CDS             10970..12349
                     /codon_start=1
                     /function="kinase"
                     /gene="spaK"
                     /gene_functions="regulatory (smcogs) SMCOG1003:sensor
                     histidine kinase (Score: 139.5; E-value: 3.3e-42)"
                     /gene_kind="regulatory"
                     /product="SpaK"
                     /protein_id="AAB91593.1"
                     /transl_table=11
                     /translation="MGIGFKGRKTLLRELVKYMVTLCISLVVLALLYIFINTIAMNTGF
                     SHPANYNEREAEKLAPKLETIDKVTADMIPDTMSYAILNKETKQKTAGTIKEKDLQLVK
                     KKIEKKPYVNYKQKGYLVIERNNEYCVLQYSLRADFSSPLLRKYLPNYELTSICILIIL
                     LIIVISIITTYFANRLRKHFETLNVITRYIKEQNLQFTPEFTHIKEFDDVIDSLIEMRD
                     ALQSSLEALWRLEKNKKEQIGALAHEIKIPITIIKGNAELLSLSMQNEEQAEYTKYILG
                     AGNQIEQYIYQLIHLSKTEDALTIHLEKASVDELTETLVKDISAYKGNKNINISFKKEN
                     LMKEAKIDWQLLHRALLNILTNAVDYTPEGGTVSVHAECDSEIFYFFVKDTGNGFSEMG
                     LKKATELFYMDDKSRHSKGHYGMGLTFAKNAVNLHNGELTLGNTIAGGAEVRVKIPLRN
                     E"
     CDS             complement(12559..12885)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs)
                     SMCOG1167:transcriptional regulator (Score: 100.1; E-value:
                     6.7e-31)"
                     /gene_kind="regulatory"
                     /note="putative protein similar to hypothetical protein
                     YvaP"
                     /product="Orf1"
                     /protein_id="AAB91592.1"
                     /transl_table=11
                     /translation="MNQSEMCPRFEKAVDILSKRWVALIVFQLLNGSQRFSEIEAALPN
                     LSGRVLSERLKELELEGVVKRDVIPETPVRIEYSLTDKGKALAPILGEISKWATDWIDP
                     SFLD"
     CDS             complement(12976..13395)
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1148:hypothetical
                     protein (Score: 61.9; E-value: 1.7e-18)"
                     /gene_kind="other"
                     /note="putative protein similar to hypothetical protein
                     YvaO"
                     /product="Orf2"
                     /protein_id="AAB91591.1"
                     /transl_table=11
                     /translation="MSPFGQQLRELRRARKLTVNQLAVYSGISSATISKIENGKRGTPK
                     PATIKKLAAVLKVPYENLMAAAGHIRAFPEEIREASESYQSIYDIYQTAVTRGAEHLPI
                     FNSQKWGYLSKQDIENLSKYFDFLALEAKKRTSSS"
     gene            complement(13424..13831)
                     /gene="yvan"
     CDS             complement(13424..13831)
                     /codon_start=1
                     /gene="yvan"
                     /gene_functions="other (smcogs) SMCOG1148:hypothetical
                     protein (Score: 59.4; E-value: 9.6e-18)"
                     /gene_kind="other"
                     /note="hypothetical"
                     /product="Yvan"
                     /protein_id="AAB91590.1"
                     /transl_table=11
                     /translation="MESFGEQLRALREERKLTVNQLATYSGVSAAGISRIENGKRGVPK
                     PATIKKLAEALKIPYEGLMYKAGYIEEVHEARAPYETKCKLLEKAEAYDLKNLALLENE
                     KWQYLNKEDLLMLDHYFSFISDEAKKRSADD"
ORIGIN
        1 gagctcccgc tcgcgctttc tgtcattatg gcaggcttgc gcacggctct cgtcattgcc
       61 atcggaatta cagcaatcgg cacatttgtc ggcgccgggg ggctcgggga tatgattgtc
      121 cgcggctcaa acgcgacaaa cggaactgcg attattctcg caggcgcgac cccgactgcg
      181 gtaatggcca taggagcaga tttgataatg gcctggattg aaaggttctt gaatccggtg
      241 aaacaaaaaa gcagaagaaa ggtaataagt gtatagagtt aagcaaaaag gattcttttc
      301 tgagagggaa aagagtcctt tttttatggt atttactggg tggatcttga tatttttttg
      361 atttttagaa tgtatagtaa aaatagagta ttgtaaatat tttggttcaa atagaataat
      421 atggaggact aagcagaggg atgtagaatt gtttttatta agaagcggat aaatatgggg
      481 ggctatagac taatatgaaa tccttatata cacctacaga ttattatatg attcgggttc
      541 ctttagtaca tcaagactta aaaaatgaga attctcagga tatcgatcag ttattacatg
      601 acctttgcaa cgattcatta tttcgggaac aaatactggt atctagcagg acactatatg
      661 aaacaataca tactttcctg caagcgccgg ataaattaaa agggaaaaaa aagcgcaact
      721 ttcaacaggc tattttgaag tatgcaacaa gaagagcaac aagaaccaca ccttttggcc
      781 ttttttcttc agtcggtata gggtcgtttt ctgataaaaa tcacttatct tttaatcaac
      841 attcatttta caaaaaggct cgtgttgatt tggagtggct ctaccaatta attagaaaat
      901 tggaaaacga atacaccgac cgactttctt ttacattaaa ttccgcttgt tatattaagg
      961 gtgaccgggc ttacttgttg tacagcacag atggaaaatc tgaagaagtt agtgttcgtg
     1021 cgacatctgt tttctatttg ataaatgaac tgtgtggtga atctgctgca tatcaagata
     1081 taatccgttg tttgatagat aactatccaa atactccaat aaataaaatt aatcagtacg
     1141 tagcagacct tattgacaaa gagttcctta tatcaaactt acggccgccg atgactgttt
     1201 cagatcaatt tcagtattta attgatcaag cggaaagccg ccatattcca aatgaactca
     1261 ttcaggcttg taaagacatt caatatcaga tagatgcata taatcggatc actatcggag
     1321 agggagagca tcagtattta aatctgattg aaacaatgaa taaactcata aaggcatcat
     1381 ctcctctgca agtagacgct gggctggcag actcctccat tcaattagat aatgaaacat
     1441 ctctcgccat aagtgaattg gcaagcatgt ttacttatat ggcttctccc tctgccaata
     1501 cattagacca cttggaaaaa taccacaatg tatttttgga acgctatgga tatgaaagag
     1561 aagttcctct cttagaaatg ctatgttcca gcactggcat tggtgctcct gctacgtaca
     1621 cgaatcctgc taatgaattc tttgaagaaa catcatttgg ggagcaattt tcaccggaaa
     1681 tgaaacaatt tttcatgaga aagtattttg aatcagttag aaagaaagct cctattcaat
     1741 tagacgacga aacattccat agaatttgca actctgaaat tgctgatgag gaaattccat
     1801 tatcatttga actcaatttc tttgttaaat tacgaaatgg gagagttaag ctttatttag
     1861 gccccaacgt cggatctacc cgcgcaggga aaacatttgg gagattttct catatgtctg
     1921 attcaatcag tgaaatcata aagaccttac ataacaagga gaaagagtta acagagtgta
     1981 atacgaaagt ttgtgaactg agtattgtgc ctaatcaaac taggtctgga aatgtaacaa
     2041 gaaatgtaag ctaccgagaa aaagagatgt ctctttttac gaacagtgct ctgcatctca
     2101 atgattccgt caaagctgaa gatattctaa ttggaatcaa taaagaccat aacttttatg
     2161 ctagacataa aacaactggc gaaattctgt cttttgagtc aaatcatatg tttaatcctt
     2221 tattaatgac caacgctgta cggtttttat tagagatttc aagagatggt aaaagaaaat
     2281 ggaatgattt cccgtggttt agtatctata gtgatttcaa gtatattcct gaaatcaaat
     2341 ataaagagat aaccttatct tgtgaacaat ggctgatata caaaaatgat ttaagcatgc
     2401 actcgaatgc atctctagaa gagataaaat ctcctttttt tgaatttcat cgtacttatg
     2461 aactgccgca aacattttat atcgttaacg cagacaatcg attattgatt gatatagaga
     2521 atgattgtac tttggatgtt tttttctggg aattgaaaaa aacgaaccat aaccagccat
     2581 tacaacttgt ggctgttgag catgatgcag atgcgttaat ggatagaaac caaaatgact
     2641 attcgggaga aatcgtcgtt ccgctgctta gaaaacaacc agaaaaacca ttgtatttac
     2701 cggttcttaa cgcaatagag ggaagtggtt ccgacagaat aaaaatgcca tttgaagact
     2761 ggctgtttat taagctttat tgtaaacaaa caagagaaga agagctaatt gcttttgaaa
     2821 tagcggattt ttataaccag atttctgatc aatatccagt cagacatttc tttatgaggt
     2881 atcgggatcc aaagcctcat ataagactta gatttaatgg aaaagccgaa gtgctgtaca
     2941 gcttgtttcc ccaattattg aattggctga aaagcttaag agaaaaagga ctggtttcag
     3001 agtctgttat cactcaatac gagcgggaga tagaacgata tggcgggcta agccttatgg
     3061 aggctgcaga acagcttttc tgtgaagaca gcaaagttgt tgaaatgatg attagaatgc
     3121 accggatgaa agatattacg ataagcaagg aaattgcagg catggtttcg gttatacagt
     3181 ttttagaaca gttcgagcta acgtttgaag aacagttaac ttttttagag agaaattcct
     3241 tacagaatga gtatcgtact gaatttaaaa aggatagaga aatgtatatt gaaatatgca
     3301 attctgacag agattgggat aatctcaaga aaacaagtga tggcggtatg ttatatgaaa
     3361 ctttgaaaac aagaaaaatg gctgcagctc attatgcatt tttaatcaaa aaggcatttg
     3421 ataacaaaga tgaagtttat tcacgtatag gaagtatcat ccatctgcat tgcaatcgtt
     3481 tattcggaac cgacagagaa ctggaaaata aaattctcac cctatgcaga cattctttat
     3541 atgcgcaacg atatcaaaag atgaatggta gtttagcatg gaagtaaagg aacaactgaa
     3601 actaaaagag ctgctgttta tcatgaaaca aatgcctaag acgttcaagt tgattttcac
     3661 cttagaaaga tcactgtttt taaagttgat tcgattcagt attattaccg gtattctgcc
     3721 aattgtttca ctatatattt cacaagaact gattaattcc cttgtgacta tccggaaaga
     3781 ggtttcaatt gttatcacca ttttcttgac atatctaggg gtatcttttt tttcggagct
     3841 catttcgcag atttccgaat tttataatgg taaatttcaa ttaaatattg gttataaact
     3901 caactataaa gtcatgaaaa agagcagtaa tttagctctc aaggattttg aaaatccaga
     3961 aatatatgac aagttagaga gggtaacgaa agagatcagt tataagccct atcagattat
     4021 tcaagccatc attacaatga ccacatcatt tgtgacgcta ctctcatcaa ttgcgttttt
     4081 aatgtcgtgg aaccctaaag tctcacttct gttgttagtt attccggtaa tttctctatt
     4141 ctatttcttg aaaattggac aggaagaatt cttcatacac tggaaacgcg cgggaaagga
     4201 aaggaaatct tggtatatca gctatatact cacacatgat ttttctttta aagaattaaa
     4261 attgtataac cttaaggatt atttattaaa caagtactgg gatatcaaaa aatcatttat
     4321 agaacaagat acaaagatat taagaaagaa aactttattg aacctaatat atgagattgc
     4381 agtgcaatta gtcggagctg tcattatatt tattgccatc atgtctgctt ttgcgggaaa
     4441 aataatggtc ggtaatgtaa tgagttatat cagatcggtt tcgctggtgc aaaatcactc
     4501 acaatctatc atgacaagca tttattcgat ctataacagc aatctctata tgaatcaatt
     4561 gtatgaattt ctggaattaa aagaagagaa aagtcaaggt cacaagaagc cgattgttga
     4621 gcctattcat tctgttgttt ttcaaaatgt cagctttatc tatcccaatc aaggagaaca
     4681 gacactgaaa catattaacg tttcattgca taaaggagaa cgtgtagcca ttgttggacc
     4741 aaacggatca gggaaaaaaa cattcattaa gctccttacc ggcttatatg aagttcacga
     4801 gggcgacatt ttgattaatg gaataaatat taaagaactg gatatggaca gttatatgaa
     4861 tcaaattgca gcgctatttc aagactttat gaaatacgaa atgacattaa aagaaaatat
     4921 aggattcggc caaattgata aactacatca aacaaacaaa atgcatgaag ttctcgatat
     4981 tgtgagagcc gatttcttaa aaagccactc ttcctatcaa ttcgatacac agctcggact
     5041 atggtttgat gaaggaagac agctatccgg tggacaatgg caaaagattg ctttggcaag
     5101 ggcttatttt agggaagctt ctttgtatat tttggacgag ccaagttctg cgctggatcc
     5161 aattgcagaa aaagaaacct tcgatacttt tttcagtttg tcaaaagata aaataggaat
     5221 atttatttct catcgattgg ttgctgctaa acttgcagat cggatcattg ttatggataa
     5281 aggagagatt gtaggaatag gcacacatga agagctatta aaaacatgtc cgctgtataa
     5341 aaaaatggat gaatccgaga actatatgaa cccactggag gaggaaggca gtaaatggaa
     5401 agaggcactg tatcaaggat agaagttgaa attgtgaaag aaatggctcg tcaaatttct
     5461 aattacgaca aagtgcttga aatagtgaat caaaaagata actttcgcag cattggcgaa
     5521 gttccgctta taccttggaa atcaactgct ttgagtcatg gtataccagg catttgtatg
     5581 ctgtatggcg aattgcacgc tcattttcct gaggaaggat gggacgacat aggacatcaa
     5641 tatttatcga ttttagtaaa tgaaataaaa gaaaaagggc tgcatactcc ttcaatgttt
     5701 tctggagcag ctggcattgg actggcagca atctgtttat cgcaacgttt cacctattac
     5761 aatggtttga tttctgatat taatgaatat ttggctgaaa ccgtacctca attgttaact
     5821 gaatttgatc agcggcaagt gtgtatgagt gactacgatg tcatagaagg agttagcgga
     5881 attgccaact atctgctgtt atttcaagaa gataaagcta tgggagattt gttaattgat
     5941 attttgaaat atctcgttag gttaactgaa gacattatcg tggatggaga aaaagttcct
     6001 ggatggcata ttccatctca acatcagttt actgatattg aaaaaaaagc ttatccttac
     6061 ggcaatttta atatgggatt agcacatggg atacctggtc ctatttgtgt tctctcttca
     6121 gcactcatac agggaattaa agtaaaggga caagaagctg cgattgaaaa aatggcgaat
     6181 ttcctactgg aattttctga aaaagagcaa gacagcttgt tttggaaagg aatcataagc
     6241 tttgaggagt atcaatacgg atctccgcca aacgcagtaa attttagcag agatgcttgg
     6301 tgttatggaa gacctggtgt atgtctagcg cttgttaaag ccggaaaagc acttcaaaat
     6361 actgaactta taaatatcgg agtacagaat ttaagataca caatatctga tatacgggga
     6421 attttttcac ctaccatatg tcatggttac agcggtatcg gtcagattct gcttgctgta
     6481 aatctactca ccggacagga gtattttaag gaagagcttc aagagattaa acaaaagatc
     6541 atgagctact atgacaagga ttatatcttt ggattccata actatgaatc aatggaaggg
     6601 gaggaagcag tacctttgca gtacgttggt ttgttggatg gagctgtagg tgtaggctta
     6661 ggggtattaa acatggaatt aggctcaaaa acagattgga caaaagcatt attaatttaa
     6721 taaaaaaagg aaaaaaatga taaaatcttg atatttgtct gttactattt aggtattgaa
     6781 aggaggtgac caatatgtca aagttcgatg atttcgattt ggatgttgtg aaagtctcta
     6841 aacaagactc aaaaatcact ccgcaatgga aaagtgaatc actttgtaca ccaggatgtg
     6901 taactggtgc attgcaaact tgcttccttc aaacactaac ttgtaactgc aaaatctcta
     6961 aataagtaaa accattagca tcaccttgct ctgactcctt gcacttctga gtgttataca
     7021 tacttatttt catagagtcg ggacaagaaa atgaagtaaa aaacgacggg tgtgaaagag
     7081 tttatattca cacccgtttt tatattcggc tttaaggagg aacacaattg tagaacggaa
     7141 gaacggttat tttcgatcat gcgttttgaa taacattcca ataaaaattc cagtctcttc
     7201 ctcaaatgca gacaaaggat gaaggactta agggtactta ccaggtttta tggttaagaa
     7261 tatttctaag aacatcatat tttttattag gaaattaata aatgttgatc actctagagt
     7321 aggcgtgtga cctgatcgca ctttttttat ttggcattga tcatggcaat ggccgcccaa
     7381 atgcttaaag tttccagttg gaaatgtttt tttgattaaa ttttgataaa agtattctag
     7441 aatggtctgc atccggaaaa aaggattctt cacatggatt catggccgtg aagaatccat
     7501 tattgcttaa catctattga ggaaaattca taagattgag gtgatgtatg ttgtttttga
     7561 aaagaagtgt taacatttta ggctgtttta ttgttttatt tatgttatct gcttgtcaat
     7621 cattaacaaa gtttaaagat aaagttgttg aagaaacaaa aaaaacaatg cattttactg
     7681 atgataacga aaacgataca tcggaaacaa tggagagcct gattgataag ggaaaacttg
     7741 atcaagtggt ctatgacgat caactttatc atctgaaaga gaaagttgat gaagacaaaa
     7801 aaggaaaagt aataggggca ataggccaaa ctttctttgt agatggtgat ggaaagagat
     7861 ggtcggaaga agaattgaaa gaaccgtata tctctaataa cccggatgaa attagagaga
     7921 aaaaaccatt acgctatggg aaggtttata gtacaaacga agattctgat gccaaagatg
     7981 aaatcattgt tgagtttaat cgtgagtatt atcgagcggt tttaataaag aatgaaaagg
     8041 aataagggag agtgtgacat gagtacagtc ttattagaaa ccaaaagtct tacaaaaaag
     8101 tttggcaaag agacagctgt tgcagatgtt tcattaactg ttcaaaaaaa cagcatttat
     8161 ggactgttgg gaccaaatgg agctggaaaa tcgacaacgc ttaaaatttt aacaggaatt
     8221 ttacggaaaa cttcaggtga aattctcttt gatggacata aatggaaaag atctgatctg
     8281 caaactatcg gatcgttaat agaagctccg cctctttacg ataatttaac tgcatttgaa
     8341 aatgtgaaag tacatgccac gctgctcggt ctttccgaag atcgcataca cagtgtgcta
     8401 aagactgtgg atatgcagca tgctggaaag aaaagagcag gccaattttc gatggggatg
     8461 aggcagcggc ttggaattgc aatcgcctta ttaaaccatc ctaaactatt aattcttgat
     8521 gaacctacaa acgggttaga tccaataggt atacaagagc tcagagacct tattcgctca
     8581 tttcctcagg aaggtataac ggtgatttta tctagtcaca tcctttcgga agtcgagcaa
     8641 atagcagatc atattgggat tatcatcgac ggtcatcttg ggtaccaggg tgaaataaac
     8701 aaggctgatg atttggagca cttgtttatg gaagttgtaa agtcaactcg tgaaaaagaa
     8761 ggtgtaaaaa gatgataaat tatataaaag ccgaaaacct aaagtttaag aggacgtttt
     8821 ccagaaaaat gattatcttt gtagctcttc ttaatatagg tttttctttt ttaatgaaca
     8881 ctcaattttt tgtaccagga acttataact ggtggtctat tatatttatg ccagtgatga
     8941 ttgctttatt ttgttcttta tctcatcaaa aagaaaaaaa ggcctctcat tataacggta
     9001 cgtattcatt gcctatagat ctagggaaat tatggtacgc taagattatt attattgcaa
     9061 tttattcact attatctcag gttgtatttt tagtttttat gttactcatg ggattcgtta
     9121 tagctgattt ttccatcatt tcgccttcta tagttgccgc gagcttattg ctttggttga
     9181 caagtctttg gcaaattccg ctctgtttat ttgtggcaaa aaagtgggga tttacaatgg
     9241 cggtaatgtt aaatttcata gggacgctag ttctcggtgt tatgccggca tctcgtgcct
     9301 tttggtggtt cattccttgg agctggccaa ttcgaatgat gtgtccaacc attggtatac
     9361 atcctaacgg tttgctgctc gaaaacaatg atcctttgtt gagttggatg gttgtgcctc
     9421 ctgccatagt gatttcaatt ttattttttt tgatactggc atttctgacc agccgttcgt
     9481 ttgccccaac aactgattac aaaatgaata agcgggaggc gatgtaaggt gaaattgctt
     9541 ttgaggtgtt tacaagctga tttccagaag agcaagcaca cctctttttt gtggctgcat
     9601 cttatcattc ctatcgtttg ttcagttata ttgattttgt atttttacgg aagagatcaa
     9661 gtggaatttc acctttataa gtcttttatg gaagctatcg cagttgcatt accgctgctc
     9721 atcggggtgc tatgtggaat gactgcatcc ttagaagaac aggcagggaa atttcatgta
     9781 ttactaggga gcacatctcc gaaaatcgtt gcttatgtga gtaaagtgtt catgctgctt
     9841 ttcttggaga tttgctctat tggtttagca ctattcatct atttcattgg attaaagttt
     9901 attttaaaag tgccggattt atcttacgat atttttataa tgggtggtgc ctggctggtt
     9961 gcaggcagtg tagtcctgta ttgtatttac ttttttatca gttgtatgtt tggaatgggg
    10021 gcttctgtcc tgataggagg agcaggcctg ctcatgacag ctctcatgaa cactggatta
    10081 ggggatgcta tatggaaatt taacccgtgg gcctggggga ttagattgtc aagtttaaat
    10141 gaagtacttc actttaaaga agtaaatcaa gggcatcatc ctttattaat gcatgaaata
    10201 aattcagggg tattgatgat ggtttttggt ataatatgta caatcattgc cagtcttata
    10261 tggtttagta aatgggaagg cagaaaaacg tttgaatagt atgagggggc cgggaaatgg
    10321 caaaaatcct tgctgtcgat gatgaaaaag atattcttgt actgatacaa aatattttgc
    10381 gccgtgacca gcatcaagtt gacattttag atcatgttca tggacagccg cctgatgtat
    10441 ttcaaggata tgatttaatt ttgttagatg tgatgatgcc ggatattgac ggttttgagt
    10501 tatgtaaaca gatacggcct ttggttgatt gtcccatttt attcctcaca gctaaaacgg
    10561 aagaggaagc gatagtaaaa ggcctcatta ccggcggaga tgattatatt acaaagccgt
    10621 tcggcgttag agagcttagt gccagagtga atgctcactt aagaagagaa cggagagata
    10681 aacatcaatc gaagagagtg atctccggtt ttttatttca ttttgatagt aaagaagtat
    10741 ttatcaataa caacaaactc aacctaacaa aaaatgaata taaaatctgt gaatttttag
    10801 ctcaacacaa agggagaact ttttcgagag aacaaattta cgaggagata tatggattag
    10861 aggggaatgc tttgtattca acaattactg aatttattcg aacgattcga aaaaaatgta
    10921 aagagcataa tgcagatccc attaaaacgg tatggggcgt tgggtacaaa tgggaatagg
    10981 ttttaagggc cggaaaacgc ttcttagaga actggtaaag tatatggtga ccctatgtat
    11041 tagcttggtc gtacttgctc tgctatatat atttataaat acgattgcaa tgaatacagg
    11101 tttctcacac ccagcgaatt ataatgaacg agaagcggaa aaacttgcgc cgaagctgga
    11161 aaccatcgac aaagtaactg ccgatatgat tcctgataca atgtcatatg ccattcttaa
    11221 caaagaaaca aaacaaaaaa cagctggaac tattaaagaa aaagatctgc agctggtaaa
    11281 aaagaagatt gaaaaaaaac catatgtcaa ttataagcaa aaaggctatt tggttataga
    11341 aagaaacaat gaatattgtg ttttgcaata ttcattgcgt gccgatttca gttcgccttt
    11401 actaagaaaa taccttccta attatgaatt aacgagtatt tgtattttga ttatcttgct
    11461 cattatagtt atctctatta taacaactta ctttgcgaat agattaagaa aacattttga
    11521 gacattgaac gtaatcactc gatatataaa agagcaaaat ttgcaattca ctcccgaatt
    11581 tacacatatt aaggaattcg atgatgtgat tgactcgttg attgaaatga gagatgctct
    11641 ccagtcctct cttgaggctc tgtggcgttt agaaaaaaac aagaaagaac aaataggtgc
    11701 tttggcacat gagatcaaga ttccgatcac cattattaag gggaatgctg aattactcag
    11761 cttgtcaatg caaaacgaag aacaagctga gtatacaaag tatattcttg gagcaggaaa
    11821 tcaaattgaa cagtatattt atcagctgat tcatctttcg aaaacggagg atgccttaac
    11881 gattcatttg gagaaagcat ctgtagatga gttgacagaa actctagtga aagatatatc
    11941 cgcctataaa ggaaataaaa atataaacat ttcgttcaaa aaagaaaatc taatgaaaga
    12001 agcgaaaata gactggcaat tgttacatag ggcattattg aatattttaa caaatgcagt
    12061 tgattataca ccggaaggcg gcaccgtctc ggtacatgca gaatgcgatt ctgagatatt
    12121 ttattttttt gtaaaagaca cgggaaatgg attttcggaa atggggttaa aaaaagccac
    12181 agaacttttt tatatggatg acaagagccg tcattctaaa ggtcattatg gaatgggatt
    12241 gacttttgca aaaaatgcag tgaatcttca taatggagaa cttactcttg ggaacactat
    12301 agccggtgga gcagaggttc gagtaaaaat acctttaaga aacgaataaa gcccatgtcc
    12361 tatctggacg tgggttattt taaatgtgaa ctgatccatc ttctcatgaa gctctttcgc
    12421 cagctgctca aattcttcgg ctaaaaacgc aatgtggggt tctactatat atcggctgag
    12481 aagtcgtttt ttacctggca aatgaaaaat aaaaaaacgg ctcttttctc gcctttgaga
    12541 tcagaaccgt tttagcaatt agtcaagaaa ggaagggtca atccaatccg tcgcccattt
    12601 agaaatctca cccaagatgg gggccaacgc cttcccttta tcggtcaatg aatattcgat
    12661 gcgaaccgga gtttcaggga tgacatcccg cttcaccact ccttcaagct caagttcttt
    12721 cagccgttct gacagaactc tgccgcttag atttggaagt gctgcttcaa tttcgctaaa
    12781 tcgctgcgac ccgttcaaga gctgaaatac gatcaaagcg acccagcgtt tactcaagat
    12841 gtcgactgct ttttcaaatc taggacacat ttctgattgg ttcatacttt tcacctcgtc
    12901 tgacactatt ataacgcaaa taacgaggga aggtatgcaa gcgtgattga taaaatggag
    12961 aaaagataca ggggattatg aagaagaggt tcgttttttt gcttccaagg ctaaaaaatc
    13021 gaagtatttg ctgagatttt caatatcctg ctttgagaga tatccccatt tttggctatt
    13081 gaaaatcggg aggtgctccg ctccgcgagt gacagctgtt tgataaatgt catatatgga
    13141 ctgatagctt tccgaagctt cgcgaatttc ttctggaaag gcccgaatat ggcctgcggc
    13201 agccatcagg ttttcgtagg gaactttgag tacggccgcc aattttttga tagtggcagg
    13261 ttttggcgtg ccccgcttac cgttttcaat tttggaaatg gtggcggagc tgatgcctga
    13321 gtacaccgca agctgattca ctgtcagttt gcgggcgcgg cgcagctccc gtaattgctg
    13381 tccgaaaggt gacatggtga tcccctcaca tttctatcaa gagttagtca tcagcagacc
    13441 gtttcttggc ttcgtctgaa ataaacgaaa aataatgatc cagcatgagc aaatcttctt
    13501 tattgagata ttgccacttt tcgttttcaa gaagagcaag gtttttcagg tcataggcct
    13561 ccgctttttc aagcagcttg cacttcgttt cataaggagc cctagcttca tgcacttctt
    13621 cgatgtaacc ggccttatac ataagccctt cataaggaat ttttaaggct tccgccagtt
    13681 ttttaatggt ggcaggtttg ggaacgcccc gtttgccgtt ttcgattctg gagatgccgg
    13741 ctgcgcttac gcctgaataa gtcgccagct ggttgactgt taattttctc tcttcacgca
    13801 atgcccgcaa ttgttcgcca aagctttcca tgtatctcac cccgtccctc actgttactc
    13861 atattttagt atgtttattg actaaaggga ataggtaggc tggaaatgtg ttgacaaaag
    13921 ttaacataag ccgtaatgta aaagtataga aaaaagtttt ccaggaatgg aaggtgagga
    13981 aagtgccact agaaaagata cagattagac gtgattatgt attgcagtat atggtcaata
    14041 atgattattc ccttaatcag cttgcgctgg aaatcggagt ctcacccgcc acacttagcc
    14101 gcgtgctgaa tggagaaaga cggccggggc agcttgtgat cggtaaaatg cttcactatt
    14161 ttaatttgaa atttgaggat cttttttact atgattttat tgacaaaagt caataaagga
    14221 accaaaaaac gcaaggagac caagcgtttt ctatctctat gaatgatgtg tcaacacaaa
    14281 ccctgtcacc agttcgttgc atgttttcca ttgttttgtc ggcagctgat gcccctgata
    14341 ttccaatctg aaaaattcgg catggctgca agctttgcgg taatagcctg aggctgggaa
    14401 ggtccagtcc cgcaggccgc catacataaa cagcattggc atgtgtaaat gaggcagctg
    14461 ttcaatgcag ttgtagcgga gcgaatcctg ataatattga tgccaatgga caaggtctgc
    14521 ttttttcata tggcctgtta ataggtttcg aagttctttg ttttttgtgt ggctgacggc
    14581 aagcattttt atgagcaaac cggcgttttt ctcgag
//
