LOCUS       BGC0000572              4516 bp    DNA     linear   BCT 23-JUN-2015
DEFINITION  Burkholderia thailandensis E444 chromosome 1, complete sequence.
ACCESSION   BGC0000572
VERSION     BGC0000572
KEYWORDS    .
SOURCE      Burkholderia thailandensis E444
  ORGANISM  Burkholderia thailandensis E444
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Burkholderia; pseudomallei group.
COMMENT     The source DNA is availble from Paul Keim (Paul.Keim@nau.edu).
            ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-07 19:54:18
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4516
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS_motif       1..84
                     /prepeptide="leader"
                     /note="peptide class: lassopeptide"
                     /note="predicted sequence: MVRLLAKLLRSTIHGSNGVSLDAVSSTH"
                     /locus_tag="BTJ_873_lassopeptide"
                     /aSTool="lassopeptides"
                     /tool="antismash"
     CDS_motif       85..144
                     /prepeptide="core"
                     /locus_tag="BTJ_873_lassopeptide"
                     /peptide="lassopeptide"
                     /predicted_class="Class II"
                     /score="0.40"
                     /molecular_weight="2049.2"
                     /monoisotopic_mass="2048.0"
                     /core_sequence="GTPGFQTPDARVISRFGFN"
                     /aSTool="lassopeptides"
                     /tool="antismash"
                     /leader_sequence="MVRLLAKLLRSTIHGSNGVSLDAVSSTH"
                     /leader_location="[0:84](+)"
     protocluster    1..4516
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[218:2628]"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      219..2628
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
     cand_cluster    1..4516
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /kind="single"
                     /product="lassopeptide"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..4516
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000572"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..4516
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lassopeptide"
                     /region_number="1"
                     /rules="(PF13471 and Asn_synthase or micJ25 or mcjC)"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            1..144
                     /locus_tag="BTJ_873"
     CDS             1..144
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (lassopeptides)
                     predicted lassopeptide"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="BTJ_873"
                     /product="hypothetical protein"
                     /protein_id="AHI78086.1"
                     /transl_table=11
                     /translation="MVRLLAKLLRSTIHGSNGVSLDAVSSTHGTPGFQTPDARVISRFG
                     FN"
     gene            219..944
                     /locus_tag="BTJ_874"
     CDS             219..944
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: PF13471"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Lasso_Fused_RRE"
                     /gene_kind="biosynthetic"
                     /locus_tag="BTJ_874"
                     /product="transglutaminase-like superfamily protein"
                     /protein_id="AHI79402.1"
                     /sec_met_domain="PF13471 (E-value: 1.8e-25, bitscore: 77.4,
                     seeds: 84, tool: rule-based-clusters)"
                     /sec_met_domain="Lasso_Fused_RRE (E-value: 2.1e-20,
                     bitscore: 60.9, seeds: 20, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTPASHCHIAVFDQAIVALDMQRSRYFLYDEACAKAFADHYLDFK
                     PIDAPHALKPLISDRIVVAASPASVPKRIADYRGWAFDTFDSGIWASRTLGERSAAGFE
                     WLPFWRIVRGAVSLKMRGFRALSALDRLARLDAGAEQRVRADGGPSRTAERYLRASIWS
                     PFRITCLQMSFALATHLRRENVPAQLVIGVRPMPFVAHAWVEIDGRVCGDEPELKKSYG
                     EIYRTPRHDERAGPFGLAA"
     gene            1021..2628
                     /locus_tag="BTJ_875"
     CDS             1021..2628
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Asn_synthase"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) lasso"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1177:asparagine synthase (glutamine-hydrolyzing)
                     (Score: 98.8; E-value: 5.1e-30)"
                     /gene_kind="biosynthetic"
                     /locus_tag="BTJ_875"
                     /product="asparagine synthase family protein"
                     /protein_id="AHI77932.1"
                     /sec_met_domain="lasso (E-value: 0.0, bitscore: 1158.1,
                     seeds: 24, tool: rule-based-clusters)"
                     /sec_met_domain="Asn_synthase (E-value: 1.3e-18, bitscore:
                     55.8, seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTLLEAGDRARAYLRDAHSRIERSLARARTLQEARDTVTRSVWGA
                     YLLVLDEAASGRRLFMPDPLHSVRLYYRTDERGRVDVDPRAANLLDRASIDWNLDYLIE
                     FACTQFGPLDETPFASVRVVPPGCALVVGPDGRCAIERAWLPRAQAAGDVRASCAAALD
                     DVYSRIAHSHPNVCAALSGGVDSSAGAIFLRKALGANAPLAAVHLYSTSSPDCYERDMA
                     ARVADSIGAQLICIDIDRHLPFSERIVRTPPAALNQDMLFLGIDRAVSNALGPSSVLLE
                     GQGGDLLFRAVPDANAVLDALRSNGWSFALRTAEKLAMLHNDSIPRILLMAAKIALRRR
                     LFGQDAPASRQTMSRLFASYAPRAAAGRSRRHAPRADAPLDESISMLDRFVSIMTPVTD
                     AAYTSRLNPYLAQPVVEAAFGLHSYDSFDHRNDRIVLREIASAHTPVDVLWRRTKGSFG
                     IGFVKGIVSHYDALRELIRDGVLMRSGRLDEAELEHALKAVRVGQNAAAISVALVGCVE
                     VFCASWQNFVTNRHAAVC"
     gene            2747..4516
                     /locus_tag="BTJ_876"
     CDS             2747..4516
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288:ABC
                     transporter related protein (Score: 219.2; E-value:
                     1.7e-66)"
                     /gene_kind="transport"
                     /locus_tag="BTJ_876"
                     /product="ABC transporter family protein"
                     /protein_id="AHI77484.1"
                     /transl_table=11
                     /translation="MKLAALRSLADVVRLMVDKETKARKLIVAGVLIEALTSAFMLLGP
                     LALKLAVDEMSRAQFDAAKAAVDIVLFAVLWSASAVSSIALLAYTGKIVHATSNTLLRR
                     ALHAQLPALASRASSDSGYVQGLLERLPYSLQVIIEGVLWKIAPVAIQLVVALALIALL
                     VPLRYAAILFAVLAAYFVFSHLSAEQYENSAATTNHAAGELSAALGDVLANAPRVVYNG
                     AVPREIDYVAARADARLDVNWRRSWLLTRAAAYQYGIIALGMAAMFALCVRDIAAHRIT
                     LGDFMLLQTYVLQFALPLGAYGFVLRQAGAALANVREALAIAPRAGGGDGDGGALAQPR
                     PGGSAAHIAVRRLAFRRAGRFAIEPMSFDLPAGSYTAIVGHNGSGKSTLAKIVAGLLPP
                     DEGGVAYDGVDLYGVAGDARHRFALYVPQDVALLNRSLRENVRYPPSTLTDDDAARLLE
                     RLAFHKDGRRIDLDGDVGEGGARLSGGQVQKVELVRLMGVDVPAIVLDETTSGLDPHSD
                     TLGIAMLRERLGRRTTLVLITHRIANVEAADQVLFLSGGRLVAAGPHRRLVDTCDEYRT
                     FWRRQPEPADAKA"
ORIGIN
        1 atggttcgac ttttggcgaa gctgcttcgt tcgacgatcc acggctccaa cggtgtcagc
       61 ctggacgcgg tttcctcgac gcacggcacc ccgggtttcc aaaccccgga cgctcgcgtc
      121 atttcgcgct tcgggttcaa ttaaatcgcg cttcgtgctc gattgagtaa cgcttcatga
      181 agctggtggg gcattttgcc ccaccggaaa aaatcaccat gacgccagcc agccattgcc
      241 atattgcggt attcgatcag gcgatcgtcg cactcgatat gcaacggtcg cgctattttc
      301 tttacgacga agcatgcgcg aaggcttttg ccgatcatta tctggatttc aagccgatcg
      361 atgcgccgca tgcattgaag ccgttgatat ccgaccggat cgtcgtcgcc gcatcgccag
      421 cgtccgtccc gaagcgcatt gccgattacc gcgggtgggc gttcgacacg ttcgattcgg
      481 gcatctgggc aagccgcacg ctcggcgagc gaagcgcggc cggcttcgag tggctgccgt
      541 tctggcgcat cgtgcgcggc gcggtgtcgc tcaagatgcg aggctttcgc gcgctctcgg
      601 cgctcgaccg tctcgctcgg ctcgacgcgg gcgcggaaca acgcgtgcgc gccgacggcg
      661 gtccgtcgcg cacggccgag cgctacctgc gcgcgtcgat ctggtcgccc tttcgcatca
      721 cgtgcctgca aatgtcgttc gcgctcgcca cgcacctgcg gcgggagaac gtgcccgctc
      781 aactggtgat cggcgtgagg ccgatgccgt tcgtcgccca tgcttgggtc gaaatcgacg
      841 ggcgcgtgtg cggcgacgaa ccggagctga aaaagagcta tggcgaaatc tatcgaacgc
      901 cgcgacatga cgaacgcgcc ggcccgttcg ggctggcggc ttgaacgggg cgcgtggacg
      961 gacgcgcgcg ggtatcgcgc gctgctgtcc ctcgacgccg cccccgaccg gccgatcggc
     1021 gtgacgctgc tcgaggccgg cgaccgcgcc cgcgcgtacc tgcgcgacgc gcactcgcgc
     1081 atcgagcgct cgctcgcgcg cgcccgcacg ctgcaggaag cgcgcgacac cgtcacgcgc
     1141 tccgtgtggg gcgcgtacct cctcgtgctg gacgaagcgg cgagcggccg gcgcctgttc
     1201 atgcccgacc cgctgcattc ggtccggctt tattaccgga cggacgagcg cgggcgtgtc
     1261 gacgtcgatc cgcgggcggc gaacctgctc gatcgcgcgt cgatcgactg gaatctcgat
     1321 tacctgatcg aattcgcgtg cacgcagttc ggtccgctcg acgaaacgcc gttcgcgtcg
     1381 gtccgcgtgg tgccgcccgg atgcgcgctc gtcgtcggcc ccgacgggcg ctgcgcgatc
     1441 gagcgcgcgt ggctgccgcg cgcgcaagcc gccggcgacg tgcgcgcatc gtgcgcggcg
     1501 gcgctcgacg acgtctattc gcgcatcgcg cacagtcatc cgaacgtgtg cgccgcgctg
     1561 tcgggcggcg tcgattcttc cgcgggcgcg atcttcctgc gcaaggcgct cggcgcgaac
     1621 gcgccgctcg ccgccgtcca cctgtactcc acgtcgtcgc ccgactgcta cgagcgggac
     1681 atggccgcgc gcgtcgccga ttcgatcggc gcgcagctca tctgcatcga catcgatcgc
     1741 cacttgccgt tctccgagcg gatcgtgcgg accccgcccg ccgcgctgaa ccaggacatg
     1801 ctatttctcg gcatcgaccg cgcggtgtcg aacgcgctcg gcccgtcgtc ggtgctgctc
     1861 gagggccagg gcggcgatct gctgttcagg gcggtgcccg acgcgaacgc ggtgctcgac
     1921 gcactgcgca gcaacggatg gtcgttcgcg ctgcgcacgg cggaaaagct cgcgatgctt
     1981 cacaacgatt cgattccgcg catcctgctg atggccgcga agatcgcgct cagaaggcgg
     2041 ctgttcggac aggacgcgcc ggcctcgcgg cagacgatgt ccaggctgtt cgcgtcgtac
     2101 gcgccgcgcg cggcggccgg gcggtcgcgc cgtcacgcgc cgcgcgctga cgcgccgctc
     2161 gacgaatcga tctcgatgct cgaccggttc gtgtcgatca tgacgcccgt caccgacgcc
     2221 gcgtacacga gccggctcaa tccgtatctc gcgcaaccgg tcgtcgaggc ggcgttcggc
     2281 ctgcacagct acgacagctt cgatcaccgc aacgaccgga tcgtgctgcg cgagatcgcg
     2341 tcggcgcaca cgcccgtcga cgtgctgtgg cgcagaacca agggatcgtt cggcatcggc
     2401 ttcgtgaagg gcatcgtgtc gcactacgac gcgttgcgcg agctgatccg cgacggcgtg
     2461 ctgatgcgca gcggcaggct cgacgaagcg gagctcgagc acgcgctcaa ggccgtgcgc
     2521 gtcggacaga acgcggccgc gatcagcgtc gcgctcgtcg gatgcgtgga ggttttttgc
     2581 gcgtcctggc agaatttcgt gacgaaccgg cacgccgcgg tatgctgacg aaccgtttcg
     2641 ccgcatcccc gatggcgcgc gcgcggccgc acccggtccc gccgcgcgcc ggaacatggc
     2701 ccttcccatc cgaaacgccg gcttgccggc ccgatcgcga atcgacatga agcttgctgc
     2761 gctacgctcg ttggcggacg tcgtccgcct gatggtcgac aaggaaacga aggcgagaaa
     2821 gctgatcgtc gccggcgtgc tgatcgaggc gctgacgtcg gccttcatgc tgctcggccc
     2881 gctcgcgttg aagctcgcgg tcgacgagat gtcgcgcgcg cagttcgacg cggcgaaggc
     2941 tgccgtcgat atcgtgctgt tcgccgtgct ctggtccgcg tccgcggtct cgtcgatcgc
     3001 gctgctcgcg tacaccggca agatcgtgca cgcgacgtcg aataccctgc tgcgccgcgc
     3061 gctgcatgcg caactgccgg cgctcgcgag ccgcgcgtcg agcgacagcg gctacgtgca
     3121 gggcctcctc gagcgcctgc cgtacagcct gcaggtgatt atcgaaggcg tgctgtggaa
     3181 gatcgcgccg gtggcgatcc agctcgtcgt cgcgctcgcg ctgatcgcgc tgctcgtgcc
     3241 gctccgatac gcggcgatcc tgtttgccgt gctggccgcc tacttcgtct tttctcacct
     3301 gtcggccgag caatacgaga acagcgcggc gacgacgaac cacgcggcgg gcgagctgtc
     3361 ggccgcgctc ggcgacgtgc tggcaaacgc gccgcgcgtc gtctacaacg gcgcggtgcc
     3421 gcgcgaaatc gactacgtcg ccgcgcgcgc cgacgcgcgc ctcgacgtca actggcgccg
     3481 gtcatggctg ctcacgcgcg cggccgccta tcagtacggc atcatcgcgc tcggcatggc
     3541 ggcgatgttc gcgctgtgcg tgcgcgacat cgccgcgcac cggatcacgc tcggcgattt
     3601 catgctgctg cagacctacg tgttgcagtt cgcgctgccg ctcggcgcgt acgggtttgt
     3661 gctccgccag gcgggcgcgg cgctcgcgaa cgtgcgcgag gcgctcgcga tcgcgccgcg
     3721 cgcgggcggc ggcgatggcg acggcggcgc gctcgcgcag ccgcgcccag gcggctcggc
     3781 cgcgcacatc gcggtgcgcc ggctcgcgtt tcgccgggcc gggcgcttcg cgatcgagcc
     3841 gatgtcgttc gatctgccgg cgggcagcta cacggcgatc gtcggccaca acggctcggg
     3901 caagtccacg ctcgcgaaga tcgtcgccgg gctgctgccg cccgacgaag ggggggtcgc
     3961 gtatgacggc gtcgacctgt acggcgtcgc cggcgacgca cgccaccgct tcgcgctgta
     4021 cgtgccgcag gacgtcgcgc tcctgaaccg ctcgctgcgg gagaacgtcc gctacccccc
     4081 gtcgacgctc accgacgacg atgccgcccg gctgctcgag cgcctcgcgt tccacaagga
     4141 cggccggcgc atcgatctcg acggcgacgt cggcgaaggc ggcgcgcgcc tgtcgggcgg
     4201 ccaggtgcag aaggtcgagc tcgtcaggct gatgggcgtc gacgtccccg cgatcgtcct
     4261 cgacgaaacg acttcggggc tcgacccgca cagcgacacg ctcggcatcg cgatgctgcg
     4321 cgagcgcctc ggcagacgga cgacgctcgt cctgatcacc caccggatcg cgaacgtcga
     4381 ggcggccgac caggtgctgt tcctgtccgg cgggcggctc gtcgcggcgg gcccgcaccg
     4441 gcgcctcgtc gacacctgcg acgaataccg gacgttctgg cgccgccagc ccgaacccgc
     4501 cgacgcgaag gcgtga
//
