LOCUS       BGC0000587             16823 bp    DNA     linear   BCT 19-DEC-2005
DEFINITION  Escherichia coli H47 microcin genetic system.
ACCESSION   BGC0000587
VERSION     BGC0000587
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
COMMENT     On or before Dec 8, 2005 this sequence version replaced AJ278866.1,
            AJ009631.2.
            ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-07 19:57:07
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 16823
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..16823
                     /country="Uruguay"
                     /db_xref="taxon:562"
                     /host="Homo sapiens"
                     /isolation_source="faeces"
                     /mol_type="genomic DNA"
                     /organism="Escherichia coli"
                     /strain="H47"
     subregion       1..16823
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000587"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..16823
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     repeat_region   1292..1439
                     /rpt_type=DIRECT
     gene            1763..2881
                     /gene="mchA"
     CDS             1763..2881
                     /codon_start=1
                     /db_xref="GOA:Q712Q2"
                     /db_xref="InterPro:IPR002213"
                     /db_xref="InterPro:IPR010610"
                     /db_xref="UniProtKB/TrEMBL:Q712Q2"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /function="participates in microcin H47 synthesis, IroB
                     homologue, glucosyltransferase"
                     /gene="mchA"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1062:glycosyltransferase, MGT family (Score: 233.7;
                     E-value: 7.5e-71)"
                     /gene_kind="biosynthetic-additional"
                     /product="MchA protein"
                     /protein_id="CAG25570.1"
                     /transl_table=11
                     /translation="MRKRILFIGPPLYGLLYPLISLAQAFRVIGHDVVISSAGKFANKA
                     AEAGLVVFDAVPGLDSEAGYRHQEELRKKSNIIGHFSFFSDEMADNLIDFAGKWRPDLI
                     VYPPLGPAGPLVAAKYRIPSVMLAVGFAHTSAHIQMLNRSLSNAYRRHGVSGPLCDLAW
                     IDVAPPSMSILKNAEEPVISMRYIPYNGGAVKETWWDRDSDRKRLLISLGTVKPMVDGL
                     ELISWVMDSANEVDADIILQLAINARTGLRKLPSNVRLVDWIPMGVFLNGADGFIHHGG
                     AGNTLTALYSGIPQIVFGEGADRSVNAEIVAMRGCGIIPDKHGLTSDLVNRLLYDDSLR
                     FCSDQVAAEMAEQPSPAEIAEVLMRKLKNNGK"
     gene            complement(3030..4295)
                     /gene="mchS1"
     CDS             complement(3030..4295)
                     /codon_start=1
                     /db_xref="InterPro:IPR000801"
                     /db_xref="UniProtKB/TrEMBL:Q712Q1"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /function="IroD homologue, enterobactin and salmochelin
                     esterase"
                     /gene="mchS1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1098:enterobactin/ferric enterobactin esterase (Score:
                     242.5; E-value: 1.5e-73)"
                     /gene_kind="biosynthetic-additional"
                     /product="MchS1 protein"
                     /protein_id="CAG25571.1"
                     /transl_table=11
                     /translation="MKNYLFQTPEDICVQLKKMTHPVTIRTTDIANFWHYLESATLPVI
                     TKSTTTENREVTFLWRSEKAVQGVYLRLNRVTDKKDVKKGLMTHIPSTDIWMLTLVLPA
                     SYRGSYSFIEIPTDMTQKDIFQLGSRFSPLPGKSDPFNKTAEINIRGFGESVLSLDMAP
                     EQKEWDDTSHKCTGILSTLHSFVAGYQRRIRLYFPQNPTSVPLGLLVLPDAEIWFDRMD
                     ITRALDMAITTGHIAPMAIMGIDNINESDRMNILGGNKELIFDIAENLIPQLYRDYPNI
                     VWAGRSNTILAGQSLGGVTALMAAIYASTTFGTIISHSPSMWWNPDQGSPILFTENDIS
                     WVSEQILSAPPKDVNIQLGVGSLEGTTVSHVQRLHQSLIAAGLESNLTVYAGGHDYAWW
                     RGAIIDALANYNCRKISDNNFV"
     gene            complement(4593..4826)
                     /gene="mchS2"
     CDS             complement(4593..4826)
                     /codon_start=1
                     /db_xref="InterPro:IPR002453"
                     /db_xref="UniProtKB/TrEMBL:Q712Q0"
                     /function="Microcin I47 precursor"
                     /gene="mchS2"
                     /product="MchS2 protein"
                     /protein_id="CAG25572.1"
                     /transl_table=11
                     /translation="MREISDNMLDSVKGGMNLNGLPASTNVIDLRGKDMGTYIDANGAC
                     WAPDTPSIIMYPGGSGPSYSMSSSTSSANSGS"
     gene            complement(4843..5277)
                     /gene="mchS3"
     CDS             complement(4843..5277)
                     /codon_start=1
                     /db_xref="UniProtKB/TrEMBL:Q712P9"
                     /function="Microcin I47 immunity protein"
                     /gene="mchS3"
                     /product="MchS3 protein"
                     /protein_id="CAG25573.1"
                     /transl_table=11
                     /translation="MYLTKKIIISMMFILPSAAFSSDPPPLQQSLEKTTYFSIGMNGFI
                     GYQSEGEKLYTHILTLDNPEEIFKNIIKNRKSTKESKIYAACGLYYLNVENIESLFNEN
                     DKQEYVSVLRGDILTKIKLNDILNSVIINGCNTKLISEHK"
     gene            complement(5432..5677)
                     /gene="mchS4"
     CDS             complement(5432..5677)
                     /codon_start=1
                     /db_xref="UniProtKB/TrEMBL:Q712P8"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /function="promotes enterobactin production"
                     /gene="mchS4"
                     /product="MchS4 protein"
                     /protein_id="CAG25574.1"
                     /transl_table=11
                     /translation="MNCDNNHRNEEFIVTFDKGNKQDNSRRKHDNFPIEVESSVELETH
                     CITNNKSASGIVTHDYDADYICGCGEIMCPGCGHDL"
     gene            5901..6020
                     /gene="mchX"
                     /operon="mch"
     CDS             5901..6020
                     /codon_start=1
                     /db_xref="GOA:O86199"
                     /db_xref="UniProtKB/Swiss-Prot:O86199"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /function="possibly involved in a regulatory loop
                     modulating its own expression and that of mchI and mchB"
                     /gene="mchX"
                     /operon="mch"
                     /product="MchX protein"
                     /protein_id="CAB54532.1"
                     /transl_table=11
                     /translation="MEFATNRVTVNDSRSALSSTLLLSLIMSATLLEYSLSMT"
     operon          5901..6542
                     /operon="mch"
     gene            6089..6298
                     /gene="mchI"
                     /operon="mch"
     CDS             6089..6298
                     /codon_start=1
                     /db_xref="GOA:O86200"
                     /db_xref="UniProtKB/Swiss-Prot:O86200"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /function="Microcin H47 immunity protein"
                     /gene="mchI"
                     /operon="mch"
                     /product="MchI protein"
                     /protein_id="CAB54533.1"
                     /transl_table=11
                     /translation="MSYKKLYQLTAIFSLPLTILLVSLSSLRIVGEGNSYVDVFLSFII
                     FLGFIELIHGIRKILVWSGWKNGS"
     gene            6315..6542
                     /gene="mchB"
                     /operon="mch"
     CDS             6315..6542
                     /citation=[4]
                     /codon_start=1
                     /db_xref="GOA:P62530"
                     /db_xref="InterPro:IPR002453"
                     /db_xref="UniProtKB/Swiss-Prot:P62530"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /function="Microcin H47 precursor"
                     /gene="mchB"
                     /operon="mch"
                     /product="MchB protein"
                     /protein_id="CAB54534.2"
                     /transl_table=11
                     /translation="MREITESQLRYISGAGGAPATSANAAGAAAIVGALAGIPGGPLGV
                     VVGAVSAGLTTAIGSTVGSGSASSSAGGGS"
     gene            6813..8363
                     /gene="mchC"
     CDS             6813..8363
                     /codon_start=1
                     /db_xref="InterPro:IPR006089"
                     /db_xref="UniProtKB/TrEMBL:Q2WEK8"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /function="participates in Microcin H47 synthesis"
                     /gene="mchC"
                     /product="MchC protein"
                     /protein_id="CAJ44956.1"
                     /transl_table=11
                     /translation="MSHQCSLSELNENLVPFTARQIKSSLIWCAEDVRNPGELQNACSY
                     IIDPDSTASAKVFHAERYGGSGIQRNGGGARCGFDGNYQVKGIGSNPLVGEGTDERHSN
                     GALGAVHAIYEALWGEVLAQILPYSAVRVRAVLLTDLYTEKAFERSGMKSRRALLVREP
                     VVRPAHFERAPYFQVKPEYSSQLIHDACRVRSVIHKLPGYLPVPPEEIDAEARTDPRIY
                     CIEGLCELARREAWQMAFCRTRFLRLTTSPSNIAMDGRLMDFNGLSCSFPGDSPADFGY
                     KLRLAELAKEPMVLMQGLSDLCLYIGKYMFDPDFTLAARLKVEEIFQKTFHEACYYCYL
                     ELLGIPGEFITQKEIPDILKQLVNSFVALLNKYCEKSHAQDIVNQDGSPLQKLVVTLIH
                     HRHNQKQALNSSIKNDVYFTVAQQCFSQTIHWLTQGSTRRQINASLLLKEIEHHTMKRL
                     QPREELRKENMCEKIAILLDNHGDDPLFLQEAISDMKNFMLKFSRDAFGYLEPIRNTV"
     gene            8389..8841
                     /gene="mchD"
     CDS             8389..8841
                     /codon_start=1
                     /db_xref="GOA:Q83ZA6"
                     /db_xref="UniProtKB/TrEMBL:Q83ZA6"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /function="participates in Microcin H47 synthesis"
                     /gene="mchD"
                     /product="MchD protein"
                     /protein_id="CAJ44957.1"
                     /transl_table=11
                     /translation="MSYIRETIRGKDEWTVYEQIGFAVSCMLYNRNYSLYPVLTIQYWT
                     EYAIQHNQIKFLFDSRGFPLAYITWAYLEADTEARLLRDPEFRLHPSEWNEDGRIWILD
                     FCCKPGFGRKVIDYLIQLQPWGEGEVRWLSRRKKIVTYIPERLHKT"
     gene            8994..10268
                     /gene="mchE"
     CDS             8994..10268
                     /codon_start=1
                     /db_xref="GOA:Q9EXN6"
                     /db_xref="InterPro:IPR003997"
                     /db_xref="InterPro:IPR006143"
                     /db_xref="InterPro:IPR006144"
                     /db_xref="InterPro:IPR011053"
                     /db_xref="InterPro:IPR011055"
                     /db_xref="UniProtKB/Swiss-Prot:Q9EXN6"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /function="membrane-fusion protein, second component of
                     Microcin H47 ABC exporter"
                     /gene="mchE"
                     /gene_functions="transport (smcogs) SMCOG1029:RND family
                     efflux transporter MFP subunit (Score: 72; E-value:
                     7.2e-22)"
                     /gene_kind="transport"
                     /product="MchE protein"
                     /protein_id="CAJ44958.1"
                     /transl_table=11
                     /translation="MFRQDALENRKMKWQGRAILLPGIPLWLIMLGSIVFITAFLMFII
                     VGTYSRRVNVSGEVTTWPRAVNIYSGVQGFVVRQFVHEGQLIKKGDPVYLIDISKSTRS
                     GIVTDNHRRDIENQLVRVDNIISRLEESKKITLDTLEKQRLQYTDAFRRSSDIIQRAEE
                     GIKIMKNNMENYRNYQAKGLINKDQLTNQVALYYQQQNNLLSLSGQNEQNALQITTLES
                     QIQTQAADFDNRIYQMELQRYELQKELVNTDVEGEIIIRALTDGKVDSLSVTVGQMVNT
                     GDSLLQVIPENIENYYLILWVPNDAVPYISAGDKVNIRYEAFPAEKFGQFSATVKTISR
                     TPASTQEMLTYKGAPQNTPGASVPWYKVIAMPEKQIIRYDEKYLPLENGMKAESTLFLE
                     KRRIYQWMLSPFYDMKHSATGPLND"
     gene            10261..12357
                     /gene="mchF"
     CDS             10261..12357
                     /codon_start=1
                     /db_xref="GOA:Q9EXN5"
                     /db_xref="InterPro:IPR001140"
                     /db_xref="InterPro:IPR003439"
                     /db_xref="InterPro:IPR003593"
                     /db_xref="InterPro:IPR005074"
                     /db_xref="InterPro:IPR011527"
                     /db_xref="UniProtKB/Swiss-Prot:Q9EXN5"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /function="ABC protein of Microcin H47 exporter"
                     /gene="mchF"
                     /gene_functions="transport (smcogs) SMCOG1288:ABC
                     transporter related protein (Score: 252.2; E-value:
                     1.7e-76)"
                     /gene_kind="transport"
                     /product="MchF protein"
                     /protein_id="CAJ44959.1"
                     /transl_table=11
                     /translation="MTNGSFRQIINQLDMRWRRRVPVIHQTETAECGLACLAMICGHFG
                     KNIDLISLRRKFNLSARGANLAGINGIAEQLGMVTRALSLELDELGALKMPCILHWDFS
                     HFVVLVSVKRNRYVLHDPARGRRYLGREEMSRYFTGIALEVWPGSEFLAETQQIRISLR
                     SLINSIYGIKRTLAKIFCLSVVIEAINLVMPVGTQLVMDHAIPAGDRGLLTLISAGLMF
                     FILLRAAVSMLRAWSSLVMSTLINIQWQSGLFNHLLRLPLAFFERRKLGDIQSRFGSLD
                     TLRATFTTCVVGAIMDSIMVVGVFVMMLLYGGYLTWIVLGFTMVYVLIRLVTYGYYRQI
                     SEETLVRGARASSYFMESLYGIATVKIQGMAGIRGTHWLNLKIDAINSGIKLTKMDLLF
                     GGINTFVAACDQVAILWLGASLVIDNQMTIGMFVAFGSFRGQFSDRVASLTSFLLQLRI
                     MSLHNERIADIALHEKEEKKPEIEIVADMSPVSLETTDLSYRYDSQSAQVFSGLNLSVA
                     PGESVAITGASGAGKTTLMKVLCGLFEPDSGKVLVNGTDIRQLGINNYHRMIACVMQDD
                     RLFSGSIRENICGFAEETDDEWMTECARASHIHDVIMKMPMGYETLIGELGEGLSGGQK
                     QRIFIARALYRKPGILFMDEATSSLDTESERFVNAAIKKMNITRVIIAHRETTLRTVDR
                     IISI"
     gene            12393..12539
                     /gene="mcmI (truncated)"
     CDS             12393..12539
                     /codon_start=1
                     /db_xref="UniProtKB/TrEMBL:Q2WEK4"
                     /gene="mcmI__truncated_"
                     /product="McmI (Microcin M immunity protein) truncated
                     protein"
                     /protein_id="CAJ44960.1"
                     /transl_table=11
                     /translation="MGEVKKDIKITVIAFVINYLFFYIPVSLYLSYYYRYNFFNLYMFF
                     YHL"
     gene            12610..12831
                     /gene="mcmA (truncated)"
     CDS             12610..12831
                     /codon_start=1
                     /db_xref="UniProtKB/TrEMBL:Q2WEK3"
                     /gene="mcmA__truncated_"
                     /product="McmA (Microcin M precursor) truncated protein"
                     /protein_id="CAJ44961.1"
                     /transl_table=11
                     /translation="MRKLSENEIKQISGGDGNDGQAELIAIGSLAGTFISPGFGSIAGA
                     YIGDKVHSWATTATVSPSMSPSGIGLSS"
     gene            complement(13347..13637)
                     /gene="mcmM (truncated)"
     CDS             complement(13347..13637)
                     /codon_start=1
                     /db_xref="UniProtKB/TrEMBL:Q2WEK2"
                     /gene="mcmM__truncated_"
                     /product="McmM (Microcin M-related protein) truncated
                     protein"
                     /protein_id="CAJ44962.1"
                     /transl_table=11
                     /translation="MFTLSFSITFIPAFLTNRQELISRGLLVPALFLAEFLILVPLYFI
                     FFRKREGLGRGSFRPDVFYSFLFNNDYSVICPLSAGSEENGRMDDLANIFF"
     repeat_region   14075..14218
                     /rpt_type=DIRECT
ORIGIN
        1 aagcttaagg cctgagcctc cgctcctgga aacactccgt cggtaaaaac ttaccgcatt
       61 gattaatgat gtgaactgaa gtcaacggag atcattcatc ctgaacctga atccggtgtt
      121 ctgtccctta tcttcccgtt ctgcttcagt tcttcactta ttccatcaat ctcatccgca
      181 agccataaca cgtcagctca ttcacgggca ggacgcattg tgggctgcgc ataacggaac
      241 atatcttatg aatgctattc cttatttcga ctatagcctg gcacccttct ggccatctta
      301 tcagaacaaa gtcatcggcg tccttgagcg tgcgctgcgt gagcagtccg gctcacggat
      361 acggcggatc ctgcttcgtc tgccgtggga acatgacaac gccttcaaca gcagacagac
      421 ctggtttggt atggatttta tcgaaaccgt cagtgcgctg atgaatgcga aacccggacg
      481 cgacctttgc tggctcctga cccgtcatcc ggaaaagccg gaataccacg tggtgctgtg
      541 cgtcagacag gagtatttcg acggccccga actggaccgg ctgattctgg atgcctggag
      601 caacgtactg ggcttcgcgt caccggggga agcggcaccg taccagaaac agataacccg
      661 ggatgtggtg ctggacagtc gttcaccgga ctgcgaagac atcctcaaag atttgatctg
      721 ggcgttcagt gatttcgccc gcgatcgtcg tggtgtgtgc gatccggaag cccgttgcct
      781 cgccggtaat cccggctatc ccggatctgc aggaccgttc tgagaatacg gctcagcacc
      841 tttccccgca ttcgcacaac cttcataaat ctcacagagg atattctgac catgttgacc
      901 tcaatgacag gccacgactg cgtgttgctg cgtgccgacg atcccctgat cgacatgaac
      961 tacatcacca gtttcaccgg tatgacagat aaatggtttt acaagctgat cagtgaaggc
     1021 catttcccga aacccatcaa gctgggccgc agcagccgct ggtacaaaag tgaagtggag
     1081 cagtggatgc agcagcgaat tgaggaatca cgaggagcag cagcatgaaa cgtgttgtga
     1141 tgccggtacg ctggcaatgt gcaaaatgcc ggcgctggta ttgcggaaat cagccctgtc
     1201 cctggtgctg gcgacattac cgttcatttt cctgctgacc ctctccggtc agccagcagg
     1261 ttgtagctct ttcctgactg attcgtcatt ccattcacat tgtttataac tggcattaca
     1321 ccggtgttga tgcgcacctt cccgtgtgtc tgcaccggct taacaaaatt caacagagtc
     1381 tgaaggagtc gtattctgtg caaataaccg aagcgttact tttagtgacg gggttatcca
     1441 atacttttct agtcagtgtt tagtatttat gcaagcgatg taagtagtaa ccgcttagac
     1501 tattgacatt catgatgcct gttaattcaa atttcaggta tgatctgtca tttattggtg
     1561 tgatactgat tccggtaggt tggcataact gtcctgtaac tcattgttca aaaaagaaag
     1621 ctcctacaaa ctgtatcctg tatctctacc agaagataag atacttggtt atttttgtgc
     1681 ataagctcac ataaaaataa tattaattat gttttcttag cgatagaaaa ataaacaggt
     1741 aagcagaaga cggacttttt taatgcgaaa acgtattctt tttattggcc caccgctgta
     1801 cggtttgtta tacccattga tttctctggc tcaggccttt cgtgtaatcg gacatgatgt
     1861 agtaattagt agtgctggca aattcgcgaa taaagcagca gaagctggac tggttgtttt
     1921 tgatgcagtt ccaggtttag attcagaggc tggatatcgc catcaggaag agttgaggaa
     1981 aaaaagtaat attattggtc atttctcttt ttttagcgat gaaatggcag ataacctcat
     2041 cgattttgca ggaaaatgga ggccagattt aatagtctat cccccgcttg gtccggcagg
     2101 cccattggtt gctgctaaat atagaattcc ttcagtgatg ctggctgttg gattcgcgca
     2161 tacatctgcc catattcaga tgttaaaccg ttctttaagc aatgcttaca ggcggcatgg
     2221 agtcagcggt ccactatgtg atttagcatg gattgatgtt gctcccccaa gtatgagcat
     2281 tcttaaaaat gctgaagaac cggttatctc aatgagatat attccttata acggaggtgc
     2341 tgtaaaggaa acatggtggg acagggattc tgatcgaaaa cgtttactca tcagccttgg
     2401 cactgtaaaa ccaatggttg atggtctgga gctgatttca tgggttatgg attctgcaaa
     2461 tgaagttgat gctgatatca ttttgcaact tgcaataaat gctcgtactg gattacgaaa
     2521 actaccatca aatgtacgtc tggttgactg gatacctatg ggtgtattcc ttaatggagc
     2581 tgatggattt attcatcatg gtggcgcagg taataccctg acagcgttgt atagtgggat
     2641 accacagatt gtgtttggcg aaggtgcaga tcgctctgtt aatgcagaaa ttgttgcgat
     2701 gcgtgggtgt gggattattc cggacaagca tggactgacc agtgatttgg taaatcgcct
     2761 gctttatgat gattcactac gcttctgttc agatcaggta gccgctgaaa tggctgaaca
     2821 acccagtcct gcagagatcg cagaggtttt gatgagaaaa ttaaaaaaca acgggaaata
     2881 attgtatagt attgctgata ttatcgacga aaaatgtcat gagtaataga atgtccttca
     2941 cttatatgat gaaggacata actaaataaa tgagtatgtc tcccgttata atgttgcaga
     3001 aagcgttatc ggtacgatga gataatcgct tacacaaagt tattatctga tatcttcctg
     3061 caattataat ttgctaatgc atcaataatt gctccgcgcc accaggcata atcatgacca
     3121 ccggcataga cagtgaggtt actttccaaa cctgctgcga ttaacgactg atgcaaccgc
     3181 tgaacatgtg agacggttgt accttctaaa gaaccgactc caagttggat atttacatct
     3241 ttcggaggcg ctgaaagtat ctgctcactt acccaggaga tatcattctc agtaaacaaa
     3301 atcgggctgc cctggtcagg gttccaccac attgaaggtg agtggctaat gattgtacca
     3361 aatgttgtcg acgcatatat agctgccatc agtgctgtca ctccaccgag gctctgaccg
     3421 gccagtatag tattagaacg accagcccat acgatattcg ggtagtctct gtataactgg
     3481 ggtatcagat tttccgctat atcaaagata agttctttat tgcctcccag tatattcata
     3541 cgatcagatt cattaatatt gtctatcccc ataattgcca ttggcgcaat atgaccagtg
     3601 gtaatggcca tatctaatgc ccgggtaata tccatccggt caaaccatat ttcagcatca
     3661 ggtaacacaa gtaatccaag aggtactgat gttggattct ggggaaaata taaacgaatc
     3721 cggcgttgat atcctgcaac aaaggaatgt aatgttgaaa gaatacctgt acatttatgg
     3781 gaagtatcat cccattcctt ttgttcagga gccatatcaa gagaaaggac tgattctccg
     3841 aatcctcgta tatttatttc tgctgttttg ttaaatggat cagatttacc gggtaatgga
     3901 gagaagcgac ttcctagttg aaatatgtct ttttgtgtca tatctgtggg aatttctata
     3961 aatgagtatg agccccgata tgaagctggt aacaccagtg tcagcatcca gatatctgtc
     4021 gaagggatat gagtcattag tccttttttg acatcttttt tatctgtaac acgattcagg
     4081 cgaagatata cgccttgcac tgctttctct gagcgccaca gaaatgtaac ctcccgattt
     4141 tctgtagtgg tgctttttgt gatcaccgga agagttgctg actcaagata gtgccagaaa
     4201 ttagcaatat ctgttgttct tattgtgaca ggatgtgtca ttttttttaa ctgtacacaa
     4261 atatcttcgg gagtctggaa aagatagttt ttcataggta tcatcttaaa ttaatccaag
     4321 tgtaagaata atacatcaaa tgacgttcat tcaaggggtg atgagtgact ttccggaatt
     4381 aaatctgttg tattgatgat aaatataaaa caggtttaat aaaatttttg ctttatactg
     4441 tagatttcac gtatgtaagt aatatatgaa aaggctctcg ttaagatgtc tggtacaaaa
     4501 tgtcagatca tatgcctttg taaagagacg acagaagtag agtcttacag gcacatcata
     4561 aataccctcc ggcatagccg gatggtattt atttaactgc cgctgtttgc actggatgtg
     4621 gaactactca tactataaga aggtccactt ccccccggat acatgatgat ggatggagta
     4681 tccggagccc agcatgctcc attagcatca atatatgttc ccatatcttt tccacgtaga
     4741 tctattacat tagtagaagc aggtaatcca ttaagattca tccctccttt cacggaatca
     4801 agcatgttat ctgatatttc tctcatttta aaaacctcct gctcatttat gttcagatat
     4861 taatttggtg ttgcaaccat ttattatcac agaattcaga atatcattca gttttatttt
     4921 tgttaaaata tcccctctta agacagacac atattcttgt ttatcatttt cattaaacaa
     4981 tgactctata ttttctacgt ttaaataata tagcccacaa gcagcataaa ttttagactc
     5041 cttagttgac tttctatttt ttattatatt tttaaatatc tcttcgggat tatctaatgt
     5101 aagaatgtgt gtgtataatt tttccccttc gctctgatag cctataaacc cattcatacc
     5161 tatagaaaaa taggttgttt tttctaacga ttgttgaagg ggaggtggat ctgatgaaaa
     5221 tgcagcagat ggtaatataa acatcatact tattataatc tttttcgtaa gatacatact
     5281 ttcccctttg gttttccatc gataaatatt cagataaaca atatatgttt tttcttgtta
     5341 atgttcatat cgatggattg ataactggaa tattcagcat aataatcaac aggcttgtac
     5401 ctgatcaggt atcagtttgc aaatatgttt gttataggtc atgaccgcaa ccaggacaca
     5461 taatttcacc acaaccacaa atataatcgg catcatagtc atgtgttact ataccggaag
     5521 ccgacttatt atttgtgata cagtgtgtct ccagctctac ggaggattct acctctatag
     5581 gaaaattatc gtgttttcgt cttgaattgt cttgcttgtt gcctttatca aaggtaacaa
     5641 tgaattcttc atttctgtga ttattatcac aattcattct tatgcctcct cacatttccc
     5701 gatgtatgac cgtcagtgct attagtgtac gttcaagtct caggttatac atgagcgaca
     5761 accatgagat cctgactaac aattgcatca gttacataac gtaaaatacc tcttgacttt
     5821 atatggacaa tatgacactt tttttgaaaa gtaaataaca atcattctct tttgtctttg
     5881 tctgggtgag gtctggtaag atggaatttg ctacaaacag ggttactgta aatgacagtc
     5941 ggtcagcact gtcatcaact ttgctgttgt ctttgatcat gagcgccact ctactggaat
     6001 attctttatc gatgacctga ctcccctgac tgaagcgcta agatttggta acttttcata
     6061 atgaaaataa agcaacaggt aggtattaat gagttataaa aaactgtacc aattgacggc
     6121 tatatttagt ttacctctta ctatcttatt ggtttcactt tcatcccttc ggattgttgg
     6181 cgaagggaat tcttatgttg acgtttttct aagctttata atatttcttg gttttattga
     6241 gctgattcat gggattcgaa agattttggt ctggtcaggc tggaaaaacg gaagttaaat
     6301 atgatggagt ttatatgcga gaaataacag aatcacagtt aagatatatt tccggggcgg
     6361 gaggtgcgcc agcgacttca gctaatgccg caggtgctgc agctattgtt ggagctctcg
     6421 ccggaatacc tggtggtcca cttggggttg tagttggagc cgtatctgcc ggtttgacaa
     6481 cagcaattgg ctcgaccgtg ggaagtggta gtgccagttc ttctgctggt ggcggtagct
     6541 aattataatt ttatttattt tacaggcact ttttcgatag tgcctgtaaa atatatccct
     6601 ttcgtttaaa gcagatagaa gatccattta taagtgtgtt tttatgttca acactttact
     6661 gatgaagata tgatttacag tcgttgataa ataattatta atttatgtgt aattaataga
     6721 gttttactgt aataaggaag aagctgtcag gacacttact tgctctttta tgaacactct
     6781 tttatgaaaa ttaaaacgga gaatactgcc agatgagtca tcagtgttca ctttctgaac
     6841 tgaatgaaaa cctggtgcct ttcactgcca ggcagatcaa gtcctcatta atctggtgtg
     6901 cagaggatgt cagaaatcca ggcgagctgc aaaatgcctg cagttatatt atcgatcctg
     6961 acagtacggc ttctgccaaa gtgttccatg cagagcgcta tggtggcagt ggtattcagc
     7021 gtaatggagg tggtgcacgt tgtgggtttg atggtaacta ccaggttaaa ggaataggaa
     7081 gtaatccgtt ggttggtgaa ggtactgacg aacgtcattc taatggtgca ctcggcgctg
     7141 ttcatgcaat atatgaggct ttgtggggag aagtactggc tcaaatatta ccttatagtg
     7201 ctgtgcgggt tcgggcggtt ttacttacag atctctatac tgaaaaggca tttgagcgct
     7261 ccggtatgaa atcacgaaga gccctgttgg tacgtgagcc tgttgttcgc ccggcgcatt
     7321 ttgaacgggc accatacttc caagtaaaac cggagtattc cagtcagtta attcacgatg
     7381 cctgtcgggt tagatctgtg atccacaagc tgccaggata tctacctgta ccaccggaag
     7441 aaattgatgc tgaagcacga actgatcccc ggatttattg cattgaggga ttatgtgaac
     7501 tggcacgtcg tgaggcctgg caaatggcat tttgtcgaac acgtttcctg agattgacaa
     7561 cttctccttc taatattgca atggatggca gattaatgga ttttaacgga ctcagttgct
     7621 cgtttccggg agattcccca gctgattttg ggtataaact aagattagct gaactggcaa
     7681 aagaaccgat ggtacttatg caagggctgt ctgatctctg cttgtatatc ggaaaatata
     7741 tgtttgaccc tgacttcact cttgcagccc gtttgaaggt tgaggagata tttcagaaaa
     7801 cttttcatga agcatgttat tactgttatc tagaactgtt gggtattcct ggagaattta
     7861 taacacaaaa agagatacct gatatattga aacaactggt taacagtttt gttgcattac
     7921 tcaataaata ctgcgagaaa tcacatgccc aagatattgt caatcaggat ggttcaccat
     7981 tgcaaaagtt ggttgtgacg ctaatccatc ataggcataa tcaaaagcag gcactgaata
     8041 gtagcatcaa gaatgatgtt tatttcaccg ttgcacaaca gtgtttttcc cagactatcc
     8101 actggctgac gcaaggcagt accagacgtc agataaatgc ttcattactc ctgaaagaaa
     8161 ttgaacatca taccatgaaa aggctgcaac ccagggaaga gctgaggaaa gagaatatgt
     8221 gcgaaaaaat tgccatcctg ctggataatc atggcgatga tccccttttt ttacaagaag
     8281 caatttctga tatgaaaaat tttatgctta agttttccag agatgcattt ggatatcttg
     8341 aaccgataag aaacacagtg taataaatat ataagggaaa tagtaatcat gtcttatata
     8401 agggaaacca tcagaggaaa agatgaatgg actgtttatg aacagatagg ttttgcggtc
     8461 agttgtatgc tctacaatcg taattacagt ctgtatccgg tgttaaccat tcaatactgg
     8521 actgaatatg cgatacagca taatcagatt aaattcctgt ttgattcacg aggttttcca
     8581 ctggcgtata taacctgggc atatcttgag gctgatacgg aagcgcgcct gctcagggat
     8641 ccagaattca ggttgcatcc gtctgaatgg aatgaagatg gaaggatctg gatcctggat
     8701 ttctgttgta aaccaggctt tggtcgaaaa gttattgact atctcataca gcttcagcca
     8761 tggggggaag gagaagtacg atggttaagc aggcgaaaga aaattgtgac atacatccct
     8821 gagcggctgc ataaaacgta gtacctctga agatacagag ataattgtaa attacggggt
     8881 aaatgcatcg ctgatactat tttgacagga ctctgtattt tctggctttg attatagcgt
     8941 ttcggttaat cagtgaaggt attacaggaa aagtgtgagt aagaggagct attttgtttc
     9001 gtcaggatgc tttagaaaac agaaaaatga agtggcaggg acgggcaata ttacttcccg
     9061 gaataccact atggttaatc atgctgggaa gcattgtgtt tattacggca tttctgatgt
     9121 tcattattgt tggtacctat agccgccgtg ttaatgtcag tggtgaggtc acaacctggc
     9181 caagagctgt caatatatat tcaggtgtac agggatttgt tgtcaggcaa tttgttcatg
     9241 aagggcagtt gataaaaaaa ggggatcctg tttatctgat tgacatcagt aaaagtacac
     9301 gtagtggtat tgtcactgat aatcatcggc gggatataga aaatcagctg gttcgtgtgg
     9361 acaacattat ttcccgtctg gaagaaagta aaaaaataac gttagatacc ctggaaaaac
     9421 aacgtctgca atacacagat gcgtttcgtc gctcatcaga tattatacag cgtgcagagg
     9481 aagggataaa aataatgaaa aacaatatgg agaattacag aaactatcag gcaaaagggc
     9541 tgattaataa agatcagtta actaaccagg tggcattata ttatcagcaa caaaacaatc
     9601 ttctcagcct gagcggacag aacgaacaga atgccctgca gataaccact ctggagagtc
     9661 agattcagac tcaggctgca gattttgata accgtatcta ccagatggaa ctgcaacggt
     9721 acgagttaca gaaagaactg gttaacactg atgtggaggg cgaaattatt atccgggcgt
     9781 tgactgacgg gaaagttgac tccctgagtg tcactgtcgg gcaaatggtc aataccggag
     9841 acagccttct gcaggttatt cctgagaaca ttgaaaacta ttatcttatt ctctgggtcc
     9901 caaatgatgc tgttccttat atttcggctg gtgacaaagt gaatattcgt tatgaagcct
     9961 ttccggcaga aaaatttggg cagttctctg ctacggttaa aactatatcc aggactcctg
    10021 cgtcaacaca ggaaatgttg acctataagg gtgcaccaca gaatacgccg ggcgcctctg
    10081 ttccctggta taaagtcatt gcgatgcctg aaaagcagat tatcagatat gacgaaaaat
    10141 acctccctct ggaaaatgga atgaaagccg aaagtacact atttctggaa aaaaggcgta
    10201 tttaccagtg gatgctttct cctttctatg acatgaaaca cagtgcaaca ggaccgctca
    10261 atgactaacg ggagtttcag acaaattata aatcagcttg atatgcgctg gcgacgtcgt
    10321 gttccggtta ttcatcagac ggagaccgct gaatgtggac tggcctgcct ggcaatgata
    10381 tgcggtcatt ttggtaagaa tattgacctg atatctcttc gccggaagtt taatctctcg
    10441 gcccgtggag caaaccttgc aggaatcaat ggaatagcgg agcagctggg gatggtcacc
    10501 cgggctcttt cactggagct ggatgaactt ggtgccctca aaatgccgtg tattctccac
    10561 tgggatttca gtcactttgt cgtgctggtc agcgtaaagc gtaaccgtta tgtactgcat
    10621 gatccggcca gaggcagaag atatctcggt cgggaggaaa tgagccggta ttttacgggc
    10681 attgcacttg aggtctggcc tggaagtgaa ttcctggcgg aaacccagca gatccgcata
    10741 agtctccgtt cactgattaa cagtatttac ggtattaaaa gaacactggc gaaaattttc
    10801 tgtctgtcag ttgtaattga agcaatcaat ctggtaatgc cggtggggac tcagctggtt
    10861 atggatcatg cgattccggc gggggacaga gggctgctga cgcttatttc tgctggcctg
    10921 atgttcttta tattgctcag ggccgcggtg agtatgctgc gtgcatggtc ctcactggtt
    10981 atgagcacgc tcatcaatat acagtggcag tcgggtctgt ttaaccatct tctcagactg
    11041 ccgctggcgt tttttgaacg ccgtaaatta ggtgatatcc agtcgcgttt tggctccctt
    11101 gacactttga gggccacctt taccacctgt gtggttgggg caatcatgga cagtattatg
    11161 gttgtggggg tttttgtgat gatgctgtta tatggaggat atcttacctg gatagtgctc
    11221 ggttttacca tggtttacgt tcttattcgt ctggtgacat acggctatta ccggcaaata
    11281 tcggaagaaa ctcttgtcag gggggcccgg gccagctcct attttatgga aagcctgtat
    11341 ggtattgcca cggtaaaaat ccaaggtatg gctgggatcc ggggaacaca ctggcttaac
    11401 ctgaaaatag atgcgatcaa ttcaggtatt aagttaacca agatggattt gctcttcggg
    11461 gggataaata cttttgttgc cgcctgtgat caggtggcga ttttatggct gggtgcaagc
    11521 cttgtgatcg ataatcagat gacaataggg atgtttgtgg catttggttc ttttcgtggg
    11581 cagttttcgg atcgggttgc ttcgctgacc agttttcttc ttcaactgag aataatgagt
    11641 ctgcataatg agcgcattgc agatattgca ctacatgaaa aggaagaaaa gaaaccggaa
    11701 attgaaatcg ttgctgacat gagcccggtt tcactggaaa ccactgattt aagctaccgg
    11761 tatgacagcc agtcagcaca ggtattcagt ggtctgaatt tgtctgtggc tccgggagaa
    11821 agtgtggcta taactggtgc ctccggtgcc ggaaaaacca cattaatgaa agtattatgt
    11881 ggactgtttg aaccagatag tggaaaagta ctggttaatg gcacggatat acgtcaactt
    11941 ggaataaata attatcaccg tatgatagcc tgtgttatgc aggacgaccg gctattttca
    12001 ggatcaattc gtgaaaatat ctgtgggttt gcagaagaaa cagacgacga atggatgaca
    12061 gaatgtgcca gagcaagtca tattcatgat gtgataatga aaatgccaat ggggtatgaa
    12121 acgttaatag gtgaactggg ggaaggtctt tccggcggtc aaaaacagcg tatattcatt
    12181 gcccgagctt tataccggaa acctggaata ttatttatgg atgaggctac aagttctctt
    12241 gatacagaaa gtgaacgttt cgtgaatgct gccataaaaa aaatgaatat cacccgggtg
    12301 attattgcac acagagaaac tacgttgaga actgttgaca ggattatttc tatttaaaat
    12361 ccactggtgt aactttgtaa ggagttttgt cgatggggga ggttaagaag gatataaaaa
    12421 taacagtgat tgcttttgtt atcaattatc tgttctttta tattccggtg tcattatatc
    12481 ttagttatta ctatagatat aattttttta atctatatat gtttttttat cacttgtagt
    12541 tacattttta tcgttgtggt taaacgtgaa tttttacttc ttcacaaatt ttatagcgaa
    12601 ggtgttgaaa tgagaaaact atctgaaaat gaaataaaac aaatatctgg aggtgacggg
    12661 aatgacgggc aggcagaatt aattgctatt ggttcacttg caggtacgtt tattagcccg
    12721 ggatttggtt ctattgcagg ggcttatata ggtgataaag tacattcatg ggcaacgact
    12781 gcgacggtta gtccctccat gtctccctca ggtataggat tatcatccta gtttggatcc
    12841 ggcagatgta catcaagtgc ttcttcgtct gcggggagtg gaaattaaac cttatattgt
    12901 taatgatgcc tatgtaatgg tttaagttag gctcctccga acgtttatca ttttatgcat
    12961 accgcatagt aacaatgcta gtccgtttaa tttttatcat atgagattgt aacagaaaat
    13021 gctctgttaa tgggaataac tgatataagc tagatattgt attcctgcta tgactgcatt
    13081 catcgccatg tgaagtaata tgggcattaa cagaccattc gactttattt gtgcgctaat
    13141 aagaactaac gatactaaaa agagcataaa aaatgtacga aagtcagtat attgtagatg
    13201 caatgcagag aatatgactg atgttacaat agcagagatg taaatattgt cattaaacca
    13261 gaattataaa gaattaaaaa acaccctcga aatacaatct cttcatatac aggaacatag
    13321 aagtacagaa gaaacataaa atggcctcag aaaaagatat ttgcgagatc atccattctt
    13381 ccgttttcct cactgccagc agataaggga caaataactg aataatcatt attaaagaga
    13441 aaagagtaaa aaacatccgg tcgaaaagaa cctcttccta atccttctct ttttcggaag
    13501 aaaataaaat acagtggaac aagaattaga aattcagcaa gaaataatgc tggaacaaga
    13561 agtcccctgg atataagttc ctgcctgttt gtaaggaatg caggaataaa ggtaatagaa
    13621 aatgataaag taaacattaa aaagcagccg ctgatgactg tttttatgtt gcattttttg
    13681 ttatttccat ttatattccc ttacttgtta tttaaaattt ttatataaca ggaagaggta
    13741 accatagaat gaacgtttct tctccggtat cagaacagcc ataattttta ttttatagtt
    13801 gttatcagtg aatgaatata aactaataga acaataaaaa gaatggagaa aaaacgacgg
    13861 gcaggacgcc atagttcata ttctctcgta tcagcctcac gtatcaacct ttctgaaacg
    13921 gtttcaattt cctttctccg ctccagaata tatttgtaat gtttcctcat gacatatacc
    13981 gaaaactgga accagattaa tatggatgga tacacaacac ataaacttct gatgatatcg
    14041 ctagggaacg tatcttgagc gggtaaaaga aagtcatata ctctgaataa aaccatcatt
    14101 tcaggtgttg atgcgcgctt tactgtgtct gcactggctt aacaaaattc aacagggttt
    14161 gaaaaggaac atttcgtgca aataaccgaa gccttaattt cagagccggg agacatccgg
    14221 cgttttgttc agcaggcggt ggaccactgg ccgcgtctgc tggcagtcca cttcacgctc
    14281 cattcggctg aaggaaacat caacgggcaa cagattcagg cattctgtac ttccttttat
    14341 cgacaactgc atgaacgtat tactgagcgt aatcacactg ccagtccatc ctctccggtg
    14401 gtattacgct ggttgcggga acaacatgaa ggagcaacaa ttcgatgcct gttgctgctc
    14461 agccagacga gtatttgtca cccgcgagcc agtgtcacag ttgatgaaca atgttcgcaa
    14521 gtggtggatt tactgcaaca tagctggcag gtgataagtg ctggcggaca atgccgggtg
    14581 gaaaggtgtt ttcgggttgc ccggggtgat acatccggtc agtatgttgc gttaaaaaca
    14641 gtcgcattgt ctctggggtt accggttgtg accgccatta cccatcgtcc ggtacagcgc
    14701 tgtacattga ttacagctca gtgaatcagc gctttctggc ttttcgtcgg tcattctgtc
    14761 aacgccacga tgtttgaccg ttatggggat gcggacgatt ccctgcacag cgttgtttca
    14821 cggtggtgga tgacgcaaca ccgctgttaa aaacagtcgt tcagtccttt gtgttaccgg
    14881 ttgtgacaac aatcagttgg taatggacgt gtgaaccatc tgcgcttccg ttgattttta
    14941 tggactgata aagttttgcc agctgaatct ttatacggaa tgctcttcag tatgcgtaca
    15001 cgaattgact atctggcgga taaatacagt tttactgagc tgaatgagtc ttcccgtctt
    15061 cgccagcagt ggcaggatgt tctggaggag tgccggcaga cagaggccgg gccagaagaa
    15121 cggctgcgta ttgcactgct gaatgtggat tacgtcacca gttttgaact gccttttcgc
    15181 ttgctgctta cccggacacc acaactgatt gccgcgctgc gtgaagaatg gggcatcagc
    15241 cagaaaaatg tggtgtttaa cgataaacgg tttggttgcg tgtacagcct gaaggccagc
    15301 ctttctggtg tgccggatac attccggtat catctgtctc accgtattcg tcgcgtggtt
    15361 gggaatgaaa acacatcatt gccatatcag caggttgccc gggaagtgaa agcgccccgt
    15421 gaacggctga agtatgcact ggaggcgggt ttactggtga ctgcactgga cggcctgttc
    15481 tggtttggca gtcagcgtat tgcggctgat gttctgaggc tgagaaaggc cggaatgccg
    15541 gtggtgacca cgaccgtgga ggtgcacgat aacctgacag gaacaacccg caaagtaccg
    15601 gcataccatc tctgatacaa ccatcagggg gaatattgct ctggcagcag cagtacgaca
    15661 ttctttatac tttatccccg tgcctgaaaa tatgtgctaa ggaaacggga atggcaaggt
    15721 ccgattatga tattattaat ctgtctctga aacatgaact gaatgagtgg ctgacagaga
    15781 gaggttatgc cggactggcg gataaccgga accgcctggc agaggtggtt acccggaaat
    15841 tgcaggacag cttttatata aacgtttcct gggatgcgct gaacacggca tacagtgaac
    15901 accctgagtg gttttcaggg cttgtctccg gagatgagaa ttaataagtg gattatgctg
    15961 ccatcaggca gtgtgattgc cggcagggat ttcgttttat tctgtttctt cccggggggc
    16021 gggtttgaat tttcccagaa agttgtacat cggtcgcatg cagggatgcg gatagccgtc
    16081 ccgcataaac accacccggg tttcctcaac actggtcacg gtgacaagcg caccatgatc
    16141 gtctttgtat cgctgattga ctttcggtaa tggaacatct tctgacataa attttagtac
    16201 ggtaacagtt aactaaattt tcatgtggta gcactgaatc aggagactgc gtttcctgat
    16261 gtgcgctgtt aattttctgt ttttacgaaa cattttcccc ttcttcctgt atattttatt
    16321 gcgctcctcc tttcatgtcc ttcccatatc ctgaaactca cttaaatgct gttgaatcgt
    16381 catgcattgc aattttcaat gactgaaatc aaaaattgct atttttttag ctaaccaact
    16441 gagcattcag tggaataatt ttctgcactt ttccattaat tatggttata aattttgcaa
    16501 tgtgctgttt ttgcgatata tgacagaata aagaactgtt catttgtgtt atatcgatcg
    16561 ataaatctgt catattgctg tagttcagat tattattcta taatgatcaa aaataagtca
    16621 gctaaaaatt ggcagattta ttgctcagca ttctggtacc ggttgatgcg ggtaaatacc
    16681 ggaagggcgg tagcgtgcca tcgcaaaaca tggtgggagg tatcgtcagg aggctggtga
    16741 ttgttggtat gacatgaata aaaggttttt cgtttgactg gaaaattatg ttgcaggaac
    16801 gagagggctg aggaacaaag ctt
//
