LOCUS       BGC0000599              6014 bp    DNA     linear   BCT 25-JUL-2016
DEFINITION  Bacillus thuringiensis strain DPC6431 thuricin operon, partial
            sequence.
ACCESSION   BGC0000599
VERSION     BGC0000599
KEYWORDS    .
SOURCE      Bacillus thuringiensis
  ORGANISM  Bacillus thuringiensis
            Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-07 19:59:23
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6014
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..858
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 152; E-value:
                     3.6e-46)"
                     /gene_kind="transport"
                     /operon="thuricin"
                     /product="ABC-transporter"
                     /protein_id="AED99781.1"
                     /transl_table=11
                     /translation="MDKILNVKGLSKKYNDFHLHHINFELKRGSITGFIGENGAGKSTT
                     IQSILNIIDYDSGEILFNGESFQQNRNDLKKRIGYVGDNNKFYDDVKLKVIRNFVKDAY
                     LEYWDDKFSEYLVKDVFNLNLNMKIKELSKGMRAKFLLTLAMSHNPDLLILDEPTSGLD
                     PIVRNQLLEILNDIAEKKGTTIFFSSHITDDIEKIAQKIIYLDNGSILLDEEKAAIFNR
                     FKKIPIDKINSIQIEKLKEIGVLVQDHIVVDKQVLKEQDIYDLNLEKVVLDEVLLLLKK
                     QKMN"
     source          1..6014
                     /db_xref="taxon:1428"
                     /mol_type="genomic DNA"
                     /organism="Bacillus thuringiensis"
                     /strain="DPC6431"
     operon          1..>6014
                     /operon="thuricin"
     protocluster    1..6014
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[2031:4793]"
                     /cutoff="20000"
                     /detection_rule="(TIGR03973 or SCIFF or (cds(PF04055 and
                     (TIGR04085 or SPASM)) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716
                     or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or
                     (YcaO or TIGR03604))))"
                     /neighbourhood="10000"
                     /product="ranthipeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      2032..4793
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(TIGR03973 or SCIFF or (cds(PF04055 and
                     (TIGR04085 or SPASM)) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716
                     or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or
                     (YcaO or TIGR03604))))"
                     /neighbourhood="10000"
                     /product="ranthipeptide"
                     /protocluster_number="1"
     protocluster    1..6014
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[1580:1730]"
                     /cutoff="20000"
                     /detection_rule="(subtilosin or thuricin or TIGR04404 or
                     Subtilosin_A or skfc)"
                     /neighbourhood="10000"
                     /product="sactipeptide"
                     /protocluster_number="2"
                     /tool="antismash"
     proto_core      1581..1730
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(subtilosin or thuricin or TIGR04404 or
                     Subtilosin_A or skfc)"
                     /neighbourhood="10000"
                     /product="sactipeptide"
                     /protocluster_number="2"
     cand_cluster    1..6014
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(TIGR03973 or SCIFF or (cds(PF04055 and
                     (TIGR04085 or SPASM)) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716
                     or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or
                     (YcaO or TIGR03604))))"
                     /detection_rules="(subtilosin or thuricin or TIGR04404 or
                     Subtilosin_A or skfc)"
                     /kind="neighbouring"
                     /product="ranthipeptide"
                     /product="sactipeptide"
                     /protoclusters="1"
                     /protoclusters="2"
                     /tool="antismash"
     subregion       1..6014
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000599"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..6014
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="ranthipeptide"
                     /product="sactipeptide"
                     /region_number="1"
                     /rules="(TIGR03973 or SCIFF or (cds(PF04055 and (TIGR04085
                     or SPASM)) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]) and not (TIGR01716
                     or subtilosin or TIGR02111 or TIGR03966 or TIGR04103 or
                     (YcaO or TIGR03604))))"
                     /rules="(subtilosin or thuricin or TIGR04404 or
                     Subtilosin_A or skfc)"
                     /subregion_numbers="1"
                     /tool="antismash"
     CDS             895..1536
                     /codon_start=1
                     /operon="thuricin"
                     /product="ABC-transporter"
                     /protein_id="AED99780.1"
                     /transl_table=11
                     /translation="MIIMFAILKKDILINQKYLLIVLLYSIIFPVILIIDGDNKYLMMS
                     FLLPFINVNFFVGKSCYIEDSVDVRNFLKMLPINHNKIVFIRYIEMIVTLALSSVYAFL
                     VQLYLMNGVSNAFLIHVNLLVFSALLIYFGIYLSVYYKINYHAAQNTLMFLFVIGVSTF
                     ILTEKVFNIKIEMINHLNYSASYIAFVISIIVLSITYLYSIKEFKFNKFA"
     CDS             1581..1730
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     sactipeptide: thuricin"
                     /gene_kind="biosynthetic"
                     /operon="thuricin"
                     /product="trn-beta"
                     /protein_id="AED99782.1"
                     /sec_met_domain="thuricin (E-value: 1.6e-41, bitscore:
                     128.8, seeds: 3, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEVLNKQNVNIIPESEEVGGWVACVGACGTVCLASGGVGTEFAAA
                     SYFL"
     CDS             1778..1921
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (sactipeptides)
                     predicted sactipeptide"
                     /gene_kind="biosynthetic-additional"
                     /operon="thuricin"
                     /product="trn-alpha"
                     /protein_id="AED99783.1"
                     /transl_table=11
                     /translation="MEVMNNALITKVDEEIGGNAACVIGCIGSCVISEGIGSLVGTAFT
                     LG"
     CDS_motif       1778..1810
                     /prepeptide="leader"
                     /note="peptide class: sactipeptide"
                     /note="predicted sequence: MEVMNNALITK"
                     /locus_tag="AED99783.1_sactipeptide"
                     /aSTool="sactipeptides"
                     /tool="antismash"
     CDS_motif       1811..1921
                     /prepeptide="core"
                     /locus_tag="AED99783.1_sactipeptide"
                     /peptide="sactipeptide"
                     /predicted_class
                     /score="26.00"
                     /molecular_weight="0.0"
                     /monoisotopic_mass="0.0"
                     /core_sequence="VDEEIGGNAACVIGCIGSCVISEGIGSLVGTAFTLG"
                     /aSTool="sactipeptides"
                     /tool="antismash"
                     /leader_sequence="MEVMNNALITK"
                     /leader_location="[1777:1810](+)"
     CDS             2032..3552
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ranthipeptide: Thuricin_rSAM_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF04055"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_12"
                     /gene_kind="biosynthetic"
                     /operon="thuricin"
                     /product="radical SAM domain protein"
                     /protein_id="AED99784.1"
                     /sec_met_domain="PF04055 (E-value: 8.8e-18, bitscore: 54.1,
                     seeds: 518, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_12 (E-value: 1.6e-10, bitscore: 30.3,
                     seeds: 84, tool: rule-based-clusters)"
                     /sec_met_domain="Thuricin_rSAM_RRE (E-value: 8.2e-42,
                     bitscore: 130.3, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSKKRLSMMPFKTDKFSYALDGNTGRVIVADKPTLYIISHFHKFE
                     KEELLKKTKGKFAELHQDYLTTYNYVSSLINMGMFYLSEKEDSDSPIDSKELAINSNQS
                     QLILILTEKCNLRCEYCIYNDKYPKEMGYSDEEMDFETAKKAVDMYYELHMERVKRGHK
                     RFPVITMYGGEPLLKFDLIKKVMEYAKGLMPDTLFYTTTNGTLLSEKMMDYFINNRIII
                     TFSIDGFKENHDRNRVFVNGMPTFERAFKNIKRLQEKKKEQNIEQIISFNCCFDQYTDV
                     YKVAKFFEEHYDLFNPFFVLYNQINPFDTLYFDWCDEQVKTGKWNFDKNNFKNAMQKIE
                     HELYEAETCDDHFQQVAGPLVMKDFVLSIRNKDGQQQITRNSCIPTSKIAVSPDGTLTL
                     CEKMCKKYPIGTVEKGLDWKAVDGVTEKLVRHFNSDSCKYCPIRTMCEACFMFLDENGR
                     IKPSFCKSKKMAVKKNLESYFAKKEKGFDVMKVYNHTSDLDSVKEMVK"
     CDS             3549..4793
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ranthipeptide: SPASM"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ranthipeptide: PF04055"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_12"
                     /gene_kind="biosynthetic"
                     /operon="thuricin"
                     /product="radical SAM domain protein"
                     /protein_id="AED99785.1"
                     /sec_met_domain="PF04055 (E-value: 1.1e-15, bitscore: 47.3,
                     seeds: 518, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_12 (E-value: 1.5e-12, bitscore: 36.9,
                     seeds: 84, tool: rule-based-clusters)"
                     /sec_met_domain="SPASM (E-value: 1.3e-08, bitscore: 24.3,
                     seeds: 166, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKYFRLYPYCHLEIGETNSCLYDISSGKMIRVNRENAELLRQCQ
                     ENVPIESINMDLGILDELIKMNLGTYYANPQFIEPFFETNDTKNRIFGKNNILRQMFIL
                     TSTDCNMNCKHCNTDSTVFRKTGCKIWPKSINLNALTQSHWRKILEAFYNLHGEELTFI
                     GGEPFLEFDFIKNIVEIAQEVGISKFSIFTNGSIINDTILNFLMENKIKVYIQIFEVDE
                     NKFKAFTNSDIPSIQIIDNIKKLNNHHLDLQLRILITRDNDNNLKKIVNTLQKETNVKD
                     IKIEFLYPKPDNSYYSKKYIPLMYDKKREFSHVNVQKMQFLHQYNPSFFGQITIRRDGK
                     VVPHPMLLTRVIGDLQQDDLFTIINTEEYQEYSTLNKEKISKCSTCAYKYNCMDDRVIE
                     NFATGDLYGMEYCNF"
     CDS             4824..>6014
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S41"
                     /gene_kind="biosynthetic-additional"
                     /operon="thuricin"
                     /product="carboxyl-terminal protease"
                     /protein_id="AED99786.1"
                     /sec_met_domain="Peptidase_S41 (E-value: 4.3e-25, bitscore:
                     77.1, seeds: 119, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNQRNLFLAESLVRIVNENYLFTGNQKRRWDRLSPLYLRKIQDSK
                     VDSIIFDIIQDMVLELHDPHTLFFQRKEFRYCFDINVQWINNELFLIKNKNGYPEDYIG
                     SKILKINSFNIIDEFKKQQKKFVGFPASMIRKAIIQNIMEGKYGAEELTILVETIDKKR
                     KTFVINAQSIKHLFDYKSNINIIKNSFKPIVFETINKDTLLIKILTFKFLGMSELFVSS
                     LRLLKGFKNIIFDIRDNSGGYISEAKQILSFIISKDIQMDYKIIQHAEEEKKFKVSSIQ
                     VTSNQISLFSKRKFFILCNGGTASSAEFIFLKGLLLSNEDLTIIGEQTAGLSGQAKIFT
                     IDEKDIIQVTTKKFLSRQGKEIKEGIQPDYTVIPLICDIINNKDTLLNFCLERFKLI"
ORIGIN
        1 atggacaaaa tattaaatgt aaaaggttta tcaaaaaaat ataatgattt tcatttacat
       61 cacattaact ttgaattaaa acggggatca ataacgggat tcatcggtga gaatggggca
      121 ggaaaatcta ctactattca atcaatacta aatatcatag attatgattc aggtgaaata
      181 ctatttaatg gagaatcttt tcaacagaat agaaatgatt taaaaaaaag aatcggatat
      241 gtaggagata acaataaatt ttatgatgac gtcaaattga aagtaatacg taactttgtc
      301 aaggatgctt atttagaata ttgggatgat aaattttctg aatatctagt taaggatgtg
      361 tttaacctta atcttaatat gaaaataaag gaattatcaa aaggtatgag agcaaaattt
      421 ttacttactt tagctatgtc acataaccca gatttattaa tactagacga accaacttca
      481 ggtctagatc ctattgtaag aaatcagcta cttgaaatct taaatgatat tgcagaaaaa
      541 aagggaacta caattttttt ctcctcccat attactgatg acattgaaaa aatagcacaa
      601 aaaatcattt atcttgataa tggttcgatt ttattggatg aagaaaaggc ggctattttt
      661 aataggttta aaaaaatacc tatcgataaa atcaattcaa tccaaattga aaaattaaaa
      721 gaaatcggag ttttagtaca agatcatata gttgtagaca aacaggtatt aaaggaacag
      781 gatatatatg atcttaatct tgaaaaggtg gttttagacg aagtgcttct actattaaag
      841 aaacaaaaaa tgaattagtt gtatagtgta atcaacttta acttgattgg atgagtgatt
      901 attatgtttg caattttaaa aaaagatata ttaattaatc aaaaatattt actaattgta
      961 ttactttatt caattatatt tcctgtcatt ttgataattg atggagataa taaatattta
     1021 atgatgagtt tcttattacc attcattaat gtgaatttct ttgtaggaaa gtcttgttac
     1081 atagaagatt cagtagatgt aagaaacttt ttgaagatgc tacctattaa tcataataaa
     1141 attgttttca ttagatatat tgaaatgatt gtaaccttgg ctctttcgag cgtatatgca
     1201 tttcttgttc aattatactt aatgaatggt gtaagtaatg ctttcttaat acatgtaaat
     1261 ctgttagttt tttcagcgct tttaatttac tttggtattt atttatctgt gtattacaaa
     1321 ataaattatc atgctgctca gaatacactt atgtttttat ttgtaatagg agtctcaact
     1381 tttatcttaa cagaaaaagt ttttaatata aaaatagaaa tgattaatca ccttaactat
     1441 agtgcatctt acattgcatt tgtaatctca ataattgtac tttctattac gtatttatat
     1501 tccattaagg aatttaaatt caataaattt gcttaaaaca ttttgaagaa gaggatctta
     1561 aaaataagga ggaattaatc atggaagttt taaacaaaca aaatgtaaat attattccag
     1621 aatctgaaga agtaggtgga tgggtagcat gtgttggagc atgtggtaca gtatgtcttg
     1681 ctagtggtgg tgttggaaca gagtttgcag ctgcatctta tttcctataa gaattaaata
     1741 aacataaaat gattaaaaaa ataaggagga attaatcatg gaagttatga acaatgcttt
     1801 aattacaaaa gtagatgagg agattggagg aaacgctgct tgtgtaattg gttgtattgg
     1861 cagttgcgta attagtgaag gaattggttc acttgtagga acagcattta ctttaggtta
     1921 aaaatatata tttacacaga atagcacaaa aacatttagt aaacctcttc ttctaaatgt
     1981 ttttttataa attcaaattt ttagtccatt attaatggga gtgaatagaa aatgtctaaa
     2041 aaaagactat caatgatgcc ctttaaaact gataaatttt catatgcatt agatggtaat
     2101 acgggtaggg taattgtagc tgataaacct acactgtaca tcatctcaca ttttcataag
     2161 tttgaaaagg aagaattatt aaaaaaaaca aaaggaaaat ttgctgaatt acatcaagat
     2221 tatcttacta catataatta tgtttcaagt ctcattaata tgggaatgtt ttatttatca
     2281 gaaaaagaag attctgattc accgattgat tcaaaggaac ttgctataaa tagtaatcaa
     2341 tcacagctta tattaatact tactgagaag tgtaatttac gttgtgaata ttgtatttat
     2401 aacgacaaat atcctaaaga aatgggatat tctgatgaag aaatggattt tgaaactgct
     2461 aagaaagctg tggatatgta ttatgaattg catatggaga gagtaaaaag aggtcataaa
     2521 agatttccgg taattacaat gtatggtggt gaaccattat taaaatttga tttgataaaa
     2581 aaggtaatgg agtatgcaaa aggacttatg cctgatactc ttttttatac aactacgaat
     2641 ggcaccttat tatctgaaaa aatgatggat tattttatta ataatagaat tattattact
     2701 tttagtattg atggttttaa agagaatcat gatcgaaaca gagtattcgt caatggaatg
     2761 cccacatttg aacgagcatt taagaacatt aaaaggcttc aagaaaagaa aaaggaacaa
     2821 aatatagagc aaattattag ttttaattgt tgttttgatc aatatacgga tgtatataaa
     2881 gtagcaaaat ttttcgagga acactatgat ttatttaatc ctttctttgt tctttataat
     2941 caaattaacc catttgacac attatatttt gattggtgcg atgaacaagt taaaactgga
     3001 aaatggaatt ttgataaaaa taatttcaag aatgctatgc agaaaattga acatgaatta
     3061 tatgaagctg aaacttgtga tgatcatttt caacaagtag ctggcccgct agtaatgaaa
     3121 gattttgttc tttcaataag aaataaggat ggtcaacaac aaattaccag aaatagttgt
     3181 attcctacta gtaaaatagc tgtttctcct gatggtactc taacattgtg tgaaaaaatg
     3241 tgtaaaaagt atccaatagg cactgttgaa aaaggcttag attggaaagc tgtagatggc
     3301 gttactgaaa aattagttcg gcattttaat agtgactcat gtaaatactg tccgattaga
     3361 acaatgtgtg aagcgtgctt tatgttcctg gatgaaaatg gtcgaattaa gccttcattt
     3421 tgcaaatcta agaagatggc agtaaagaaa aatttagaaa gttattttgc aaaaaaagaa
     3481 aaaggctttg atgtaatgaa ggtttacaat catactagtg atcttgattc tgtcaaggag
     3541 atggtaaaat gaaaaaatat ttcagattgt atccatattg tcacttagag ataggggaaa
     3601 caaattcatg tctatatgat atcagtagcg gtaaaatgat aagagtaaac agagaaaatg
     3661 ctgagttatt aagacaatgt caagagaatg tacctattga atccattaat atggatttgg
     3721 gtattttaga tgagttaata aaaatgaatt taggtacgta ttatgctaat ccacaattca
     3781 ttgagccttt ttttgaaact aatgatacga aaaacagaat attcggtaag aataatatat
     3841 taagacaaat gttcatttta acttccacag attgtaacat gaattgtaag cactgtaata
     3901 cggattcaac tgtttttaga aaaacagggt gtaagatttg gccaaaaagt attaatttaa
     3961 atgcattaac ccaatcgcac tggagaaaaa ttttagaagc attttataat ctacatggag
     4021 aagagctaac atttattggt ggggaaccat ttttagaatt tgattttatt aaaaatattg
     4081 tagaaattgc ccaggaagtt ggcatttcaa aattttctat attcactaat ggtagcatta
     4141 tcaatgatac tatacttaat ttcttgatgg aaaataaaat aaaagtgtac atacaaatct
     4201 ttgaagtaga tgaaaataaa tttaaagcat ttactaattc agatatccct tccatacaaa
     4261 ttatagataa tataaaaaaa ttaaacaacc atcacctaga tctgcaactt agaatattga
     4321 tcacaaggga taatgacaat aatcttaaaa agatagttaa tacacttcaa aaagaaacta
     4381 atgttaaaga tattaaaatt gagtttctat atccaaaacc agataatagt tattattcta
     4441 aaaagtatat tcctttaatg tatgataaaa aaagagagtt ttcacatgtt aatgtgcaaa
     4501 aaatgcaatt tttacatcag tataatccaa gtttttttgg ccaaataacg ataagaaggg
     4561 atggcaaagt agtaccacat cctatgttgt taactagggt tataggtgat ctccagcaag
     4621 atgatttatt tacaatcatt aatactgaag aatatcaaga gtacagtact ctcaataaag
     4681 agaaaatatc caaatgttca acatgcgcat acaagtataa ttgtatggat gaccgagtaa
     4741 ttgaaaattt tgctaccggt gatttgtatg gaatggaata ttgtaacttt taagttaaat
     4801 ccaatatttt aggagtattc aatatgaatc aaaggaattt atttttagct gaaagtttgg
     4861 ttcgaattgt taacgaaaat tatttattta caggcaatca aaaacggaga tgggacagac
     4921 ttagtcctct ttatctaaga aaaatccaag attctaaagt agattctata atttttgata
     4981 ttattcaaga catggtttta gaattacatg atccacatac tttatttttt caacggaaag
     5041 aatttcgtta ttgctttgat attaatgtac agtggattaa taatgagtta tttctaataa
     5101 aaaataaaaa tggatatcct gaagattata taggtagtaa gatattaaaa ataaattctt
     5161 ttaatataat agatgagttt aaaaagcagc aaaagaaatt tgtaggcttt cctgcaagta
     5221 tgataaggaa agccattata caaaatataa tggaaggaaa atatggagct gaagaattaa
     5281 cgatacttgt tgaaacaatt gataaaaaaa gaaaaacatt cgtgatcaac gcacaatcta
     5341 taaaacattt atttgattat aaaagtaata ttaatataat taaaaatagc tttaaaccga
     5401 ttgtttttga aactataaac aaagatacgc tgttaataaa aattttaact tttaagtttc
     5461 ttggtatgag cgaattattc gtctctagtt taagattact gaaaggattt aaaaatatta
     5521 tttttgatat tagggataat tcaggaggat atatttcaga agcaaaacaa atattatcct
     5581 ttattatttc taaggatatt caaatggact ataaaataat acagcatgct gaagaggaaa
     5641 agaaatttaa agtttcttca attcaagtca ctagcaatca aatttcactt ttttcaaaac
     5701 gaaaattctt cattttatgt aatggtggta cagcaagttc ggccgagttc atatttctaa
     5761 aaggattgct gctatctaat gaagatttaa ccattatcgg agaacaaaca gctggtttat
     5821 caggacaagc aaagatattt actattgacg aaaaagatat tattcaagtc acaacaaaga
     5881 aatttcttag taggcaggga aaagaaataa aagagggaat acagcctgat tatacagtta
     5941 ttcctttaat atgtgatatt attaataata aagatacact tttgaacttt tgtcttgaaa
     6001 gatttaaatt aatt
//
