LOCUS       BGC0000677              5068 bp    DNA     linear   BCT 29-OCT-2015
DEFINITION  Streptomyces melanosporofaciens genes for geranylgeranyl diphosphate
            synthase, geranylgeranyl diphosphate cyclase, P450, complete cds,
            strain: MI614-43F2.
ACCESSION   BGC0000677
VERSION     BGC0000677
KEYWORDS    .
SOURCE      Streptomyces melanosporofaciens
  ORGANISM  Streptomyces melanosporofaciens
            Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
            Streptomyces; Streptomyces violaceusniger group.
COMMENT     ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2022-11-07 20:14:28
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5068
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..5068
                     /db_xref="taxon:67327"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces melanosporofaciens"
                     /strain="MI614-43F2"
     subregion       1..5068
                     /aStool="mibig"
                     /contig_edge="True"
                     /label="Terpene"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..5068
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     CDS             121..1200
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1182:Polyprenyl synthetase (Score: 267.4; E-value:
                     2.2e-81)"
                     /gene_kind="biosynthetic-additional"
                     /product="geranylgeranyl diphosphate synthase"
                     /protein_id="BAI44337.1"
                     /transl_table=11
                     /translation="MTTPTLSPGRLDADTVRKSVDVVLEDFLTAKAHTTPQHHLPYLSG
                     LLKDFLSGGKRIRPLLCVTGWQAVGGGEDTEPVFRVAACLEMFHAFALIHDDVMDDSDT
                     RRGRPTIHRTLAALCATDRRPEQIERFGVSGAVLLGDLALTWSDELLHSAGLTPVQFDA
                     VLPLLSEMRTEVMLGQYLDLQATGELTDDVEATLTVNRYKTAKYTIERPLHVGAAIAGA
                     GPEAMEAFTAYALPLGEAFQLRDDLLGVYGDPESTGKSQLDDLRAGKNTTLIALALRGS
                     DSTQAARLRSLIGNPLLDERDAATIQEIFAATTARDAVEQMIDDRRTQALRALDDAPFT
                     ADAVNALKQIARLATVRNS"
     CDS             1197..2120
                     /codon_start=1
                     /product="geranylgeranyl diphosphate cyclase"
                     /protein_id="BAI44338.1"
                     /transl_table=11
                     /translation="MTTGLSTAGAQDIGRSSVRPYLEECTRRFQEMFDRHVVTRPTKVE
                     LTDAELREVIDDCNAAVAPLGKTVSDERWISYVGVVLWSQSPRHIKDMEAFKAVCVLNC
                     VTFVWDDMDPALHDFGLFLPQLRKICEKYYGPEDAEVAYEAARAFVTSDHMFRDSPIKA
                     ALCTTSPEQYFRFRVTDIGVDFWMKMSYPIYRHPEFTEHAKTSLAARMTTRGLTIVNDF
                     YSYDREVSLGQITNCFRLCDVSDETAFKEFFQARLDDMIEDIECIKAFDQLTQDVFLDL
                     IYGNFVWTTSNKRYKTAVNDVNSRIQ"
     CDS             2117..3496
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1034:cytochrome P450 (Score: 438.3; E-value:
                     5.7e-133)"
                     /gene_kind="biosynthetic-additional"
                     /product="P450"
                     /protein_id="BAI44339.1"
                     /sec_met_domain="p450 (E-value: 1.7e-84, bitscore: 272.2,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRERGPVTPAKSSAPPERPWTTGTAPGSVPLLGHTMALWRRPLQF
                     LASLPAHGDLVEVRLGPSRAYLACHPELVRQVLLNPRVFDKGGVFDKARQLLGNSLSVS
                     RGEDHRYQRRMIQPAFHTPKIAAYTAAVADDTRAAIGSWEPGRTLDISDTMHALLMRVA
                     ARTLFSTGIDEATIDEARHCLRIVSDGIYKRTMAPLGIMEKLPTPGNRRYDRANARLRQ
                     IVDEMIRERRRSGADHGDLLSTLLRAEHPETGKGLDDGEVLDQVVTFLVAGSETTASTL
                     AFVFHLLGAHPEVEKRVHAEIDEILEGRSPTFEDLPSLEYTRGVITESLRLYPPSWMAM
                     RVTAAETELGGRTVPAGTMILYSAQALHHNPELFPDPERFDPERWLGDRAKEVERGALL
                     PFGAGSHKCIGDVLALTETALIVATIASRWRLRPVPGTTLRPEPKATLEPGPLPMVCEP
                     R"
     CDS             3561..4940
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1034:cytochrome P450 (Score: 432.7; E-value:
                     2.8e-131)"
                     /gene_kind="biosynthetic-additional"
                     /product="P450"
                     /protein_id="BAI44340.1"
                     /sec_met_domain="p450 (E-value: 2.2e-82, bitscore: 265.2,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKDFFRMRTAQQPATRHWRHTVAPGGLPLAGHALLMARKPLQFLA
                     SLPAHGDLVELRLGPRPVYLPCHPELVQQVLVNARVYDTGGPVKEKAKPILGNGLITSD
                     WADHRRQRRLVQPAFHTARIAKYAEVMERECEAESTAWTARRPIDVSHEMLALTARVTA
                     RALFSTDMAPHAVAEIQHCLPIVVEGAYRQAIDPTGLLAKLPLAANRRFDDALARLNQL
                     IDRMIDDYKASDDGDRGDVLSALFAAQDDETGGTMSDQEIHDQVMTLLLAGIETTASAL
                     TWAWFLLGRNPGAEAALHAEVDEVLGGRAPRYADVPRLAYTQRVFSEALRLFPPAWLFT
                     RTTTETTELGGRRLPPASDVLISPYVLHRDPALFPRPDSFDPDRWLPERAKEVTRGSYL
                     PFGGGSRKCIGDVFGMTEATLALAAIAGRWRMRPIPGTKIRPRPQMSLTAGPLRMIPEP
                     R"
ORIGIN
        1 tgccgtacag agcacggacg gcaatccatc tttttccata caaagcaagg gtttccgcgt
       61 gcctgttttc ttctccttaa tcaacggata tcgacatttc gacgtggagg agtcagtcgc
      121 atgaccacac ccacgctcag ccccggccgc ttggatgcgg ataccgtccg taagtcggtg
      181 gacgtcgtac tcgaagattt cctgacggcc aaggcccaca ccaccccgca acaccatctg
      241 ccgtatctct ccgggctgct caaggatttc ctgtccggcg gaaaacgcat acggccgctg
      301 ttgtgtgtca ccggctggca ggccgtcggc ggtggcgagg acaccgagcc ggtgttccgg
      361 gtggccgcct gcctggagat gttccacgcg ttcgcgctga tccacgacga cgtcatggac
      421 gacagcgaca cccgccgcgg ccggccgacc atccaccgca ccctggccgc gctgtgtgcc
      481 accgaccgca ggcccgagca gatcgagcgg ttcggggtca gcggggcggt gctgctcggc
      541 gacctcgccc tcacctggtc cgacgagctc ctgcactccg ccggtctgac gcccgtccag
      601 ttcgacgccg tactgccgct gctgtcggag atgcgcaccg aggtgatgct gggccagtac
      661 ctcgatctcc aggccaccgg ggagctgacg gacgacgtcg aggccaccct cacggtcaac
      721 cggtacaaga ccgccaagta caccatcgag cgtccgctgc acgtcggagc cgccatcgcg
      781 ggcgccggcc cggaggccat ggaggcgttc accgcctacg ccctgccgct cggcgaggcg
      841 ttccagctcc gcgacgatct gctgggcgtg tacggcgatc cggagtccac cggcaagtcc
      901 cagctggacg atctgcgggc cgggaagaac accaccctga tcgcgctcgc cctgcgcggc
      961 tcggacagca cccaggcggc gcggctgcgc agcctcatcg gcaatccgct gctcgacgag
     1021 cgggacgccg cgaccattca ggagatcttc gccgccacca ccgcccggga tgccgtcgag
     1081 cagatgatcg acgaccgccg gacacaggcc ctgcgggccc tcgacgacgc cccattcacc
     1141 gcggacgcgg tcaacgcgct gaagcagatc gcccgcctgg ccactgtgag gaactcatga
     1201 cgacaggact ttccacggcc ggggcccagg acatcggccg gagcagcgtc cgcccgtacc
     1261 tggaggagtg cacccgccgc ttccaggaga tgttcgaccg ccatgtcgtc acccggccca
     1321 ccaaggtcga attgaccgac gccgaactgc gcgaggtcat cgacgactgc aacgcggcgg
     1381 tggccccgct cggcaaaacg gtttccgatg agcgctggat ctcctatgtg ggcgtggtgc
     1441 tgtggtccca gagcccccgt catatcaagg acatggaggc gttcaaggcc gtctgcgtcc
     1501 tgaactgcgt gaccttcgtg tgggacgaca tggatcccgc actccacgac ttcggcctct
     1561 tcctgcccca gctgcgcaag atctgcgaga agtactacgg ccccgaggac gcggaggtcg
     1621 cgtacgaggc ggcacgcgcc ttcgtcacca gtgaccatat gttccgcgac tcgcccatca
     1681 aggcggcgct gtgcaccacg tccccggagc agtatttccg tttccgggtc accgacatcg
     1741 gcgtcgactt ctggatgaag atgtcgtatc cgatctaccg gcatccggag ttcaccgaac
     1801 acgccaagac gagcctggcc gcgcgcatga ccacccgcgg actcaccatc gtcaacgact
     1861 tctactccta tgaccgcgag gtctccctgg gccagatcac caactgtttc cggctctgcg
     1921 atgtgagcga tgagacggcg ttcaaggagt tcttccaggc ccggctcgat gacatgatcg
     1981 aggacatcga gtgcatcaag gcgttcgacc agctgacgca ggacgtcttc ctcgatctga
     2041 tctacggcaa cttcgtctgg accaccagca acaagcgcta caagacggcc gtcaacgacg
     2101 tcaactctcg catccagtga gggaacgggg acccgtgact cccgcgaaga gctccgcacc
     2161 gccggagcgc ccctggacca ccggcacggc gcccggctcg gtgccgctgc tggggcacac
     2221 catggcgctc tggcgccgtc cgctccagtt cctggcctcc ctgcccgccc acggcgatct
     2281 ggtggaggtc aggctggggc ccagccgcgc ctatctggcc tgccatcccg agctggtccg
     2341 tcaggtgctg ctcaatcccc gggtgttcga caagggcggg gtcttcgaca aggcgcggca
     2401 actgctcggg aacagcctct cggtctcccg gggcgaggac caccgctacc agcggcggat
     2461 gatccagccc gcgttccaca ccccgaagat cgccgcctac accgccgcgg tcgccgatga
     2521 cacccgcgcc gcgatcggct cctgggagcc ggggcgcacc ctggacatca gcgacaccat
     2581 gcacgccctg ctgatgcggg tggcggcccg gacgctgttc tccaccggga tcgacgaggc
     2641 gacgatcgac gaggcgcgcc actgtctgcg catcgtctcg gacggcatct acaagcgcac
     2701 gatggcgcct ttggggatca tggagaagct ccccaccccg ggcaaccggc gctatgaccg
     2761 ggccaatgcc cggctgcggc agatcgtgga cgagatgatc cgcgagcgcc ggcgctccgg
     2821 cgccgaccac ggcgatctgc tctccacgct gctgcgcgcc gaacaccccg agacgggtaa
     2881 gggcctcgac gacggcgagg tcctcgacca ggtggtcacc ttcctggtgg ccgggagcga
     2941 gaccaccgcc tcgaccctgg ccttcgtctt ccatctcctg ggcgcccacc cggaggtgga
     3001 gaagcgggtg cacgccgaga tcgacgagat cctggagggc cgctccccca ccttcgagga
     3061 cctgccgtcc ctggagtaca cccgcggggt catcaccgag tcgctgcggc tgtatccgcc
     3121 gtcctggatg gcgatgcggg tcacggcggc cgagaccgag ctcggcggcc ggaccgtccc
     3181 ggcgggcacg atgatcctct acagcgccca ggcgctgcac cacaaccccg aactgttccc
     3241 ggaccccgag cggttcgacc ccgagcgctg gctcggcgac cgcgccaagg aggtggagcg
     3301 cggggcgctg ctcccgttcg gcgcgggcag ccacaagtgc atcggggacg tcctggcgct
     3361 gaccgagacc gcgctcatcg tggcgacgat cgcgagccgg tggcgtctcc gcccggtgcc
     3421 cgggacgacg ttgcgccccg agccgaaggc gacgctcgaa cccggcccgc tgcccatggt
     3481 gtgcgagccg cgctgacgac gtccggtccg gggtccgccc cgggccgggc gccccgcacc
     3541 ttccgtaccc cacctcgcga gtgaaggact tcttccgcat gaggaccgcg cagcaaccgg
     3601 cgacgcgcca ctggcggcac accgtcgccc ccggcgggct tccgctggcc ggccatgccc
     3661 tgctcatggc ccgcaaaccg cttcagttcc tggcctccct gcccgcccac ggcgatctgg
     3721 tcgagctccg gctgggaccc cggcccgtct atctgccctg tcatcccgag ctggtccagc
     3781 aggtgctggt gaacgcgcgg gtctacgaca ccggcgggcc ggtcaaggag aaggccaagc
     3841 ccatcctggg caacggcctg atcacctccg actgggcgga ccaccggcgg cagcgccggc
     3901 tggtgcagcc ggcgttccac accgcgcgga tcgccaagta cgccgaggtg atggagcggg
     3961 agtgcgaggc cgagtcgacg gcgtggaccg cgcgccggcc catcgacgtc agccacgaaa
     4021 tgctcgcgct caccgcgcgg gtgaccgccc gtgcgctctt ctcgaccgat atggcgccgc
     4081 atgccgtggc cgagatccag cactgtctgc ccatcgtcgt ggagggcgcc taccggcagg
     4141 cgatcgaccc caccgggctg ctggccaagc tcccgctcgc ggcgaaccgg cgcttcgacg
     4201 acgcgctcgc ccggctcaac cagctcatcg accgcatgat cgacgactac aaggcgtcgg
     4261 acgacggcga ccgcggcgat gtgctgtccg cgctcttcgc ggcacaggac gacgagaccg
     4321 gcggcacgat gtcggaccag gagatccacg accaggtcat gaccctgctg ctggccggga
     4381 tcgagaccac cgcctcggcg ctgacctggg cctggttcct gctcggccgc aatcccgggg
     4441 ccgaggccgc gctgcacgcc gaggtcgacg aggtgctggg cgggcgcgcc ccgcgctacg
     4501 ccgacgtccc ccggctcgcg tacacccagc gggtgttcag cgaggcgctg cggctcttcc
     4561 cgcccgcgtg gctgttcacc cggaccacca ccgagaccac cgagctcggc gggcggcggc
     4621 tgcccccggc gtcggacgtg ctgatcagcc cgtatgtgct gcaccgcgat ccggccctct
     4681 tcccccgccc ggactccttc gacccggacc gctggctccc cgagcgggcc aaggaggtga
     4741 cgcgcggctc gtatctgccg ttcggcggtg gcagccggaa gtgcatcggc gacgtcttcg
     4801 gcatgacgga ggcgacgctc gccctggcgg cgatcgcggg ccgctggcgg atgcgcccga
     4861 ttccggggac gaagatccgg cccaggccgc agatgagcct cacggccggt ccactgcgga
     4921 tgattccgga accacgctga acgccgggcc ccacagaccc cgcggaccgc ggtgggtggc
     4981 caccgcggtc cgccgcgccg ggccggccgg ggcggttcgc cgagtgctcc tggccggggc
     5041 ggttcgccga gtgctcctgg ccgggggc
//
