LOCUS       BGC0000829             10094 bp    DNA     linear   BCT 28-NOV-2000
DEFINITION  Chromobacterium violaceum violacein biosynthetic gene cluster,
            complete sequence.
ACCESSION   BGC0000829
VERSION     BGC0000829
KEYWORDS    .
SOURCE      Chromobacterium violaceum
  ORGANISM  Chromobacterium violaceum
            Bacteria; Proteobacteria; Betaproteobacteria; Neisseriales;
            Chromobacteriaceae; Chromobacterium.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-07 20:44:45
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 10094
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..10094
                     /db_xref="taxon:536"
                     /mol_type="genomic DNA"
                     /organism="Chromobacterium violaceum"
     protocluster    1..10094
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[3299:6296]"
                     /cutoff="20000"
                     /detection_rule="(indsynth or dmat or indole_PTase)"
                     /neighbourhood="10000"
                     /product="indole"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      3300..6296
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(indsynth or dmat or indole_PTase)"
                     /neighbourhood="10000"
                     /product="indole"
                     /protocluster_number="1"
     cand_cluster    1..10094
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(indsynth or dmat or indole_PTase)"
                     /kind="single"
                     /product="indole"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..10094
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000829"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..10094
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="indole"
                     /region_number="1"
                     /rules="(indsynth or dmat or indole_PTase)"
                     /subregion_numbers="1"
                     /tool="antismash"
     CDS             123..1751
                     /codon_start=1
                     /note="ORF1"
                     /product="unknown"
                     /protein_id="AAD51807.1"
                     /transl_table=11
                     /translation="MKAWRFLLCAALSLPAASHAAYPEDLKLATWNAMLLPQALYPNYG
                     QMRRVELMAAAPILQAQDVLVFQELQDNAASDKLLALLRPRFPYQTPVIGRAQQGWDGT
                     EGWNGMKPEDGGVAIASRWPIVEKRQYLFQTPGCSWDGQALKGFAYARIAVNGQFYHVI
                     GTHLQSEDSGCANHADIGVRQAQLREIAAWVQSRHLPVEETVIVAGDMNIDRYKSAEYR
                     AMLDILQAGEPRYAGMPHSFDTAGNGIALERYGARGGDAPEYLDYILTAERPPPARGLA
                     QPGAGRAVAAVDGAVGGGEADLRLRRFQRPLPGAGLRLGRCRDADALAERAAGRLPPDQ
                     PAEPGQRPLCPGRRRQRRLAEDRRGGGGRAGALQSVQQLFHARQRLHPFRRIRAAGARR
                     PSRLVLDLVGRRGGQPVRVLHRPGAAQSFRRAAPDQSQPAGRLPAGRRRGVVQGLGARG
                     RLLPDRVGWRQPCRPAVSVAAGVWGWGTIPAADGRIAAVSGLARTIAFRQTPLRDCLAF
                     VSNSPRHALTLGTVSAALSDNMVDE"
     misc_feature    1128..1130
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    1167..1169
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            1969..3225
                     /gene="vioA"
     CDS             1969..3225
                     /codon_start=1
                     /function="involved in early steps of violacein
                     biosyntheis"
                     /gene="vioA"
                     /note="putative L-amino acid oxidase; VioA mutants fail to
                     produce pigmented violacein intermediates; putative FAD
                     monooxygenase of the PHEA/TFDB family"
                     /product="tryptophan 2-monooxygenase"
                     /protein_id="AAD51808.1"
                     /transl_table=11
                     /translation="MKHSSDICIVGAGISGLTCASHLLDSPACRGLSLRIFDMQQEAGG
                     RIRSKMLDGKASIELGAGRYSPQLHPHFQSAMQHYSQKSEVYPFTQLKFKSHVQQKLKR
                     AMNELSPRLKEHGKESFLQFVSRYQGHDSAVGMIRSMGYDALFLPDISAEMAYDIVGKH
                     PEIQSVTDNDANQWFAAETGFAGLIQGIKAKVKAAGARFSLGYRLLSVRTDGDGYLLQL
                     AGDDGWKLEHRTRHLILAIPPSAMAGLNVDFPEAWSGARYGSLPLFKGFLTYGEPWWLD
                     YKLDDQVLIVDNPLRKIYFKGDKYLFFYTDSEMANYWRGCVAEGEDGYLEQIRTHLASA
                     LGIARERIPQPLAHVHKYWAHGVEFCRDSDIDHPSALSHRDSGIIACSDAYTEHCGWME
                     GGLLSAREASRLLLQRIAA"
     misc_feature    1987..2085
                     /gene="vioA"
                     /note="Region: NADPH binding site"
     gene            3300..6296
                     /gene="vioB"
     CDS             3300..6296
                     /codon_start=1
                     /function="involved in early steps of violacein
                     biosynthesis"
                     /gene="vioB"
                     /gene_functions="biosynthetic (rule-based-clusters) indole:
                     indsynth"
                     /gene_kind="biosynthetic"
                     /note="VioB mutants fail to produce pigmented violacein
                     intermediates"
                     /product="polyketide synthase-like"
                     /protein_id="AAD51809.1"
                     /sec_met_domain="indsynth (E-value: 0.0, bitscore: 1589.0,
                     seeds: 14, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSILDFPRIHFRGWARVNTPTANRDPHGHIDMASNTVAMAGEPFD
                     LARHPTEFHRHLRSLGPCFGLDGRADPEGPFSLAEGYNAAGNNHFSWENATVSHVQWDG
                     GEADRGDGLVGARLALWGHYNDYLRTTFNRARWVDSDPTRRDAAQIYAGQFTISPAGAG
                     PGTPWLFTADIDDSHGARWTRGGHIAERGGHFLDEEFGLARLFQFSVPKDHPHFLFHPG
                     PFDSEAWRRLQLALGDDDVLGLTVQYALFNMSTPPQPNSPVFHDMVGVVGLWRRGELAS
                     YPAGRLLRPRQPGLGDLTLRVSGGRVALNLACTIPFSTRAAQPSAPDRLTPDLGAKLPL
                     GDLLLRDEDGALLARVPQALYQDYWTNHGIVDLPLLREPRGSLTLSSELAEWREQDWVT
                     QSDASNLYLEAPDRRHGRFFPESIALRSYFRGEARARPDIPHRIEGMGLVGVESRQDGD
                     AAEWRLTGLRPGPARIVLDDGAEAIPLRVLPDDWALDDATVEEVDYAFLYRHVMAYYEL
                     VYPFMSDKVFSLADRCKCETYARLMWQMCDPQNRNKSYYMPSTRELSAPKARLFLKYLA
                     HVEGQARLQAPPPAGPARIESKAQLAAELRKAVDLELSVMLQYLYAAYSIPNYAQGQQR
                     VRDGAWTAEQLQLACGSGDRRRDGGIRAALLEIAHEEMIHYLVVNNLLMALGEPFYAGV
                     PLMGEAARQAFGLDTEFALEPFSESTLARFVRLEWPHFIPAPGKSIADCYAAIRQAFLD
                     LPDLFGGEAGKRGGEHHLFLNELTNRAHPGYQLEVFDRDSALFGIAFVTDQGEGGALDS
                     PHYEHSHFQRLRELSARIMAQSAPFEPALPALRNPVLDESPGCQRVADGRARALMALYQ
                     GVYELMFAMMAQHFAVKPLGSLRRSRLMNAAIDLMTGLLRPLSCALMNLPSGIAGRTAG
                     PPLPGPVDTRSYDDYALGCRMLARRCERLLEQASMLEPGWLPDAQMELLDFYRRQMLDL
                     ACGKLSREA"
     gene            6298..7587
                     /gene="vioC"
     CDS             6298..7587
                     /codon_start=1
                     /function="involved in late steps of violacein
                     biosynthesis"
                     /gene="vioC"
                     /gene_functions="other (smcogs) SMCOG1087:hypothetical
                     protein (Score: 154.4; E-value: 8.7e-47)"
                     /gene_kind="other"
                     /note="putative FAD monooxygenase of the PHEA/TFDB family;
                     VioC mutants produce aqua pigmented violacein intermediate;
                     similar to Homo sapiens kynurenine 3-monooxygenase"
                     /product="monooxygebase"
                     /protein_id="AAD51810.1"
                     /transl_table=11
                     /translation="MKRAIIVGGGLAGGLTAIYLAKRGYEVHVVEKRGDPLQDLSSYVD
                     VVSSRAIGVSMTVRGIKSVLAAGIPRAELDACGEPIVAMAFSVGGQYRMRELKPLEDFR
                     PLSLNRAAFQKLLNKYANLAGVRYYFEHKCLDVDLDGKSVLIQGKDGQPQRLQGDMIIG
                     ADGAHSAVRQAMQSGLRRFEFQQTFFRHGYKTLVLPDAQALGYRKDTLYFFGMDSGGLF
                     AGRAATIPDGSVSIAVCLPYSGSPSLTTTDEPTMRAFFDRYFGGLPRDARDEMLRQFLA
                     KPSNDLINVRSSTFHYKGNVLLLGDAAHATAPFLGQGMNMALEDARTFVELLDRHQGDQ
                     DKAFPEFTELRKVQADAMQDMARANYDVLSCSNPIFFMRARYTRYMHSKFPGLYPPDMA
                     EKLYFTSEPYDRLQQIQRKQNVWYKIGRVN"
     misc_feature    6304..6396
                     /gene="vioC"
                     /note="Region: NADPH binding site"
     gene            7587..8708
                     /gene="vioD"
     CDS             7587..8708
                     /codon_start=1
                     /function="involved in late steps of violacein
                     biosynthesis"
                     /gene="vioD"
                     /note="putative FAD monooxygenase of the PHEA/TFDB family;
                     VioD mutants produce blue pigmented violacein intermediate
                     (deoxyviolacein)"
                     /product="hydroxylase"
                     /protein_id="AAD51811.1"
                     /transl_table=11
                     /translation="MKILVIGAGPAGLVFASQLKQARPLWAIDIIEKNDEQEVLGWGVV
                     LPGRPGQHPANPLSYLDAPERLNPQFLEDFKLVHHNEPSLMSTGVLLCGVERRGLVHAL
                     RDKCRSQGIAIRFESPLLEHGELPLADYDLVVLANGVNHKTAHFTEALAPQVDYGRNKY
                     IWYGTSQLFDQMNLVFRTHGKDIFIAHAYKYSDTMSTFIVECSEETYARARLGEMSEEA
                     SAEYVAKVFQAELGGHGLVSQPGLGWRNFMTLSHDRCHDGKLVLLGDALQSGHFSIGHG
                     TTMAVVVAQLLVKALCTEDGVPAALKRFEERALPLVQLFRGHADNSRVWFETVEERMHL
                     SSAEFVQSFDARRKSLPPMPEALAQNLRYALQR"
     misc_feature    7596..7682
                     /gene="vioD"
                     /note="Region: NADPH binding site"
ORIGIN
        1 ggatcgggct ggggggagtg tattgaaaat gtaaatggca tgttaatttt ctgataatta
       61 accatattga aagaaaactg ctccagcctt tgccgcttcc tccacccacc aaaggatgtc
      121 cgatgaaagc ctggcgtttt ctgctgtgcg cggcgctgtc gctgcccgcc gcctctcatg
      181 ccgcctatcc cgaagacctg aagctggcca cctggaacgc gatgctgctg ccgcaggcgc
      241 tgtatcccaa ttacggccag atgcggcgcg tcgagctgat ggccgccgcg ccgattctgc
      301 aagcccagga cgtgctggtg ttccaggaac tgcaggacaa cgccgcgtcg gacaagctgc
      361 tggcgctgct gaggccgcgc tttccctacc agacgccggt gataggccgc gcgcaacagg
      421 gctgggacgg caccgaaggg tggaatggca tgaagcccga ggacggcggc gtcgccatcg
      481 ccagccgctg gcccatcgtc gagaagcggc agtacctgtt ccagacgccc ggctgcagct
      541 gggacgggca ggcgctgaag ggcttcgcct acgccaggat agccgtgaac ggccagtttt
      601 atcatgtgat cggcacccat ctgcagtcgg aagacagcgg ctgcgccaat cacgccgaca
      661 tcggcgtgcg ccaggcgcag ctgcgcgaga tcgccgcctg ggtgcagtcg cgccatctgc
      721 cggtagagga gacggtgatc gtcgccggcg acatgaacat cgaccgctac aagagcgccg
      781 aataccgggc gatgctggac atcctgcagg ccggcgagcc tcgttacgcc ggcatgccgc
      841 acagcttcga caccgccggc aacggcatcg cgctggagcg ctacggcgcg cgcggcggcg
      901 acgcgcccga gtacctggac tacatcctga cggctgaaag gccaccgcca gcccgcggct
      961 tggcacaacc aggcgctgga cgcgccgtcg ccgcagtgga cggcgcagtc ggcggtggcg
     1021 aagcagacct acgcctacgc cgatttcagc gaccactacc cggtgcaggc cttcgcctgg
     1081 gccgatgccg cgacgccgac gcgctcgctg agcgcgcagc cgggcgctta ccgccagatc
     1141 agcctgcaga acctggccag cggccgttat gtccaggccg gcgacgccag cgacggctgg
     1201 ctgaagaccg acgcggcggc ggcgggcgcg cgggcgcgct tcaatctgtc caacaacttt
     1261 tccatgcgcg acaacggctg catccgttcc ggcgaatacg tgcggctgga gcgcgccgac
     1321 cgtcccggct ggttctggac ctggtggggc ggcgtggggg gcaaccagta cgcgtactac
     1381 accgcccagg ggccgctcaa tcgttccgcc gagctgcgcc tgatcaatca cagccggccg
     1441 gacggctgcc tgcaggacgg cgacgtggtg tcgttcaagg attgggcgcg cgcggccgac
     1501 tactacctga ccgcgtgggt tggcggcagc catgccgacc agctgtatct gtggcagccg
     1561 gcgtctgggg atggggaacg attccggctg cggatgggcg catcgccgca gtatctggac
     1621 tggcgcggac gattgctttt cgccaaacgc cgctgaggga ttgcttggct tttgtatcca
     1681 attctccacg tcatgccctg acccttggaa cagtatcagc cgcattgtca gacaatatgg
     1741 ttgatgaatg agtccggttg aactaatgtg gcttgaaatt acaaaagcat tttattgtta
     1801 aaataaaatg tcaaacgatt ttttgtgccc ggattgaccg ggcatgacga ggaaacgaaa
     1861 ggcagcatcc cgattccaag gcgcgagtgc cgtaggtttt cgccgcccat gcccgtccgc
     1921 cgttgccgcg cggcgggcgt gagttgacta gtcaaaagga cattcgcgat gaagcattct
     1981 tccgatatct gcattgtcgg cgccggcatc agcggcctga cctgcgccag ccatctgctc
     2041 gattcgcccg cttgccgcgg cctgtcgctg cgcatcttcg acatgcagca ggaggcgggc
     2101 ggccgcatcc gctcgaagat gctggatggc aaggcttcga tagagctggg cgcggggcga
     2161 tactccccgc agctgcaccc gcatttccag agcgcgatgc agcattacag ccagaagagc
     2221 gaggtgtatc cgttcaccca gttgaaattc aagagccatg tccagcagaa gctgaagcgg
     2281 gcgatgaacg agttgtcgcc caggctgaaa gagcatggca aggaatcctt tctccagttc
     2341 gtcagccgct accagggcca tgacagcgcg gtgggcatga tccgctccat gggctacgac
     2401 gcgctgttcc tgcccgacat ctcggccgag atggcctacg acatcgtcgg caagcacccg
     2461 gaaatccaga gcgtgaccga taacgacgcc aaccagtggt tcgcggcgga aacgggcttt
     2521 gcgggcctga tccagggcat caaggccaag gtcaaggctg ccggcgcgcg cttcagcctg
     2581 ggttaccggc tgctgtcggt gaggacggac ggcgacggct acctgctgca actggccggc
     2641 gacgacggct ggaagctgga acaccggacc cgccacctga tcctggccat ccctccgtcg
     2701 gcgatggccg ggctcaatgt cgacttcccc gaggcgtgga gcggcgcgcg ctacggctcg
     2761 ctgccgctgt tcaagggttt cctcacctac ggcgagccct ggtggctgga ctacaagctg
     2821 gacgaccagg tgctgatcgt cgacaacccg ctgcgcaaga tctacttcaa gggcgacaag
     2881 tacctgttct tctacaccga cagcgagatg gccaattact ggcgcggctg cgtggccgaa
     2941 ggcgaggacg gctacctgga gcagatccgc acccatctgg ccagcgcgct gggcatcgcc
     3001 cgcgagcgca ttccccagcc cctcgcccat gtgcacaagt attgggcgca tggcgtggag
     3061 ttctgccgcg acagcgatat cgaccatccg tccgcgctca gccaccgcga cagcggcatc
     3121 atcgcctgtt cggacgccta caccgagcac tgcggctgga tggagggcgg cctgctcagc
     3181 gcccgcgaag ccagccgtct gctgctgcag cgcatcgccg cgtgaacggt ccggccgccg
     3241 catcgcgtcg ccgcccggtt ccgggcggcg cttgtcagcc atgaccgttc gggaaacaca
     3301 tgagcattct ggattttcca cgcatccatt tccgcggctg ggcgcgggtc aacacgccca
     3361 ccgccaaccg cgatccgcat ggccacatcg acatggccag caatacggtg gccatggccg
     3421 gcgaaccgtt cgacctcgcg cgccatccga ccgagttcca tcgccacctg cggtcgctgg
     3481 ggccgtgttt cggcctggac ggccgggctg acccggaagg gccgttcagc ctggccgagg
     3541 gctacaacgc ggccggcaac aaccacttct cctgggagaa cgccaccgtc agccacgtgc
     3601 agtgggatgg cggcgaagcg gaccgcggcg acggcctggt cggcgccagg ctggcgctgt
     3661 gggggcatta caacgactac ctgcgcacca ccttcaaccg cgcgcgttgg gtggacagcg
     3721 accccacccg ccgcgacgcg gcgcagatct acgccggaca gttcacgatc agcccggccg
     3781 gcgccggacc gggcacgccc tggctgttca ccgccgacat cgacgacagc cacggcgcgc
     3841 gctggacgcg cggcggccac atcgccgagc gcggcggcca tttcctggac gaggagttcg
     3901 gcctggcgcg gctgttccag ttctcggtgc ccaaagacca tccgcacttc ctgttccacc
     3961 cggggccgtt cgattccgaa gcctggcgca ggctgcagct ggcgctgggg gacgacgacg
     4021 tgctcggtct gacggtgcag tacgcgctgt tcaatatgtc gacgccgccg caacccaact
     4081 cgccggtgtt ccacgacatg gtcggcgtgg tcggcctgtg gcggcgcggc gaactggcca
     4141 gctacccggc cggccggctg ctgcgtccgc gccagcccgg gctgggcgat ctgacgctgc
     4201 gcgtaagcgg cggccgcgtg gcgctgaatc tggcctgcac cattccgttc tccacccggg
     4261 cggcgcagcc gtccgcgccg gacaggctga cgcccgatct cggggccaag ctgccgttgg
     4321 gcgacctgct gctgcgcgac gaggacggcg cgttgctggc gcgggtgccg caggcgctgt
     4381 accaggatta ctggacgaac cacggcatcg tcgacctgcc gctgctgcgc gagcccaggg
     4441 gctcgctgac gctgtccagc gagctggccg aatggcgcga gcaggactgg gtcacgcagt
     4501 ccgacgcctc caatctttat ttggaagcgc cggaccgccg ccacggccgt ttctttccgg
     4561 aaagcatcgc gctgcgcagc tatttccgcg gcgaggcccg cgcgcgcccg gacattcccc
     4621 accggatcga ggggatgggt ctggtcgggg tggagtcgcg ccaggacggc gatgccgccg
     4681 aatggcggct gaccggcctg aggcccggcc cggcgcgcat cgtgctcgac gacggcgcgg
     4741 aggcgatccc gctgcgggtg ctgccggacg actgggcgct ggacgacgcg acggtggagg
     4801 aggtcgatta cgccttcctg taccggcacg tgatggccta ttacgagctg gtctacccgt
     4861 tcatgtccga caaggtgttc agcctggccg accgctgcaa gtgcgagacc tacgccaggc
     4921 tgatgtggca gatgtgcgat ccgcagaacc ggaacaagag ctactacatg cccagcaccc
     4981 gcgagctgtc ggcgcccaag gccaggctgt tcctcaaata cctggcccat gtcgagggcc
     5041 aggccaggct gcaggcgccg ccgccggccg ggccggcgcg catcgagagc aaggcccagc
     5101 tggcggccga gctgcgcaag gcggtggatc tggagctgtc ggtgatgctg cagtacctgt
     5161 acgccgccta ttccattccc aattacgccc agggccagca gcgggtgcgc gacggcgcgt
     5221 ggacggcgga gcagctgcag ctggcctgcg gcagcggcga ccggcgccgc gacggcggca
     5281 tccgcgccgc gctgctggag atcgcccacg aggagatgat ccattacctg gtggtcaaca
     5341 acctgctgat ggcgctgggc gagccgttct acgccggcgt gccgctgatg ggcgaggcgg
     5401 cgcggcaggc gttcggcctg gacaccgaat tcgcgctgga gccgttctcc gagtcgacgc
     5461 tggcgcgctt cgtccggctg gaatggccgc acttcatccc cgcgccgggc aaatccatcg
     5521 ccgactgcta cgccgccatc cgccaggcct tcctcgatct gcccgacctg ttcggcggcg
     5581 aggccggcaa gcgcggcggc gagcaccacc tgtttctcaa cgagctgacc aaccgcgccc
     5641 atcccggcta tcagctggag gtgttcgacc gcgacagcgc gctgttcggc atcgccttcg
     5701 tcaccgacca gggcgagggc ggagcgctgg actcgccgca ttacgagcat tcgcatttcc
     5761 agcggctgcg ggagttgtcg gcccgcatca tggcgcagtc cgcgccgttc gagccggcgt
     5821 tgccggcgct gcgcaacccg gtgctggacg agtcgccggg ctgccagcgc gtggcggacg
     5881 gacgggcgcg cgcgctgatg gcgctgtacc agggcgtgta cgagctgatg ttcgcgatga
     5941 tggcgcagca cttcgcggtc aagccgctgg gcagcctcag gcgctcgcgg ctgatgaacg
     6001 cggcgatcga cctgatgacc ggcctgctca ggccgctgtc ctgcgcgctg atgaacctgc
     6061 cgtcgggcat cgccggacgc accgccgggc cgccgctgcc ggggccggtg gatacccgca
     6121 gctacgacga ctacgcgctg ggttgccgga tgctggcgcg gcgctgcgag cgcctgctgg
     6181 agcaggcgtc gatgctggag ccgggctggc tgcccgacgc gcaaatggaa ctgctggatt
     6241 tctaccgccg gcagatgctg gatttggctt gtggaaagct ttctagagag gcctgaaatg
     6301 aaaagagcaa tcatagtcgg aggcgggctc gccggcgggc tgaccgccat ctacctggcg
     6361 aagcgcggct acgaggtcca cgtggtggaa aagcgcggcg atccgctgca ggacctgtct
     6421 tcctacgtgg atgtggtcag ctcgcgggcg ataggcgtca gcatgaccgt gcgcggcatc
     6481 aagtcggtgc tggcggccgg cattccgcgc gcggagctgg acgcctgcgg cgaacccatc
     6541 gtggcgatgg cgttttccgt cggcggccag taccggatgc gggagctcaa gccgctggag
     6601 gatttccgcc cgctgtcgct gaaccgcgcg gcgtttcaga agctgctgaa caagtacgcc
     6661 aacctggccg gcgtccgcta ctacttcgag cacaagtgcc tggacgtgga cctggatggc
     6721 aagtcggtgc tgatccaggg caaggacggc cagccgcagc gcttgcaggg cgatatgatc
     6781 atcggcgccg acggcgcgca ctcggcggtg cggcaggcga tgcagagcgg gctgcgccgc
     6841 ttcgaattcc agcagacttt cttccgccac ggctacaaga cgctggtgct gccggacgcc
     6901 caggcgctgg gctaccgcaa ggacacgctg tatttcttcg gcatggactc cggcggcctg
     6961 ttcgccggcc gcgccgccac catcccggac ggcagcgtca gcatcgcggt ctgcctgccg
     7021 tacagcggca gccccagcct gaccaccacc gacgagccga cgatgcgcgc ctttttcgac
     7081 cgttacttcg gcggcctgcc acgggacgcg cgcgacgaga tgctgcgcca gttcctggcc
     7141 aagcccagca acgatctgat caacgtccgt tccagcacct tccactacaa gggcaatgtg
     7201 ctgctgctgg gcgacgccgc ccacgccacc gcgcctttcc tcggccaggg catgaacatg
     7261 gcgctggagg acgcgcgcac cttcgtcgag ctgctggacc gccaccaggg agaccaggac
     7321 aaggcctttc ccgagttcac cgagctgcgc aaggtgcagg ccgacgcgat gcaggacatg
     7381 gcgcgcgcca actacgacgt gctcagctgc tccaatccca ttttcttcat gcgggcccgc
     7441 tacacccgct acatgcatag caagtttccc ggcctttacc cgccggacat ggcggagaag
     7501 ctgtacttca cgtccgagcc gtacgacaga ctgcagcaga tccagagaaa acagaacgtt
     7561 tggtacaaga tagggagggt caactgatga agattctggt catcggcgcg gggccggccg
     7621 gcctggtgtt cgccagccaa ctgaaacagg cgcgtccgct gtgggcgata gacatcattg
     7681 aaaagaacga cgagcaggaa gtgctgggct ggggcgtggt gctgcccggc cggcccggcc
     7741 agcatccggc caatccgctg tcctacttgg acgcgccgga gaggctgaat ccgcagttcc
     7801 tggaagactt caagctggtc caccacaacg agcccagcct gatgagcacc ggcgtgctgc
     7861 tgtgcggcgt ggagcgccgc ggcctggtgc acgccttgcg cgacaagtgc cgctcgcagg
     7921 gcatcgccat ccgcttcgaa tcgccgctgc tggagcatgg cgagctgccg ctggccgact
     7981 acgacctggt ggtgctggcc aacggcgtca atcacaagac cgcccacttc accgaggcgc
     8041 tggcgccgca ggtggactac ggccgcaaca agtacatctg gtacggcacc agccagctgt
     8101 tcgaccagat gaacctggtg ttccgcaccc acggcaaaga cattttcatc gcccacgcct
     8161 acaagtactc ggatacgatg agcaccttca tcgtcgagtg cagcgaggag acctatgccc
     8221 gcgcccgcct gggcgagatg tcggaagagg cgtcggccga atacgtggcc aaggtgttcc
     8281 aggccgagct gggcggccac ggtctggtga gccagcccgg cctcggctgg cgcaacttca
     8341 tgaccctgag ccacgaccgc tgccacgacg gcaagctggt gctgctgggc gacgcgctgc
     8401 agtccggcca cttctccatc ggccacggca ccacgatggc ggtggtggtg gcgcagctgc
     8461 tggtgaaggc gctgtgcacc gaggacggcg tgccggccgc gctgaagcgc ttcgaggagc
     8521 gcgcgctgcc gctggtccag ctgttccgcg gccatgccga caacagccgg gtctggttcg
     8581 agacggtgga ggagcgcatg cacctgtcca gcgccgagtt cgtgcagagc ttcgacgcgc
     8641 gccgcaagtc gctgccgccg atgccggaag cgctggcgca gaacctgcgc tacgcgctgc
     8701 aacgctgagg aggccgcatg gaaaaccggg aaccgccgct gctgccggcg cgctggagca
     8761 gcgcctatgt gtcgtactgg agtccgatgc tgccggatga ccagctgacg tccggctact
     8821 gctggttcga ctacgagcgc gacatctgtc ggatagacgg cctgttcaat ccctggtcgg
     8881 agcgcgacac cggctaccgg ttgtggatgt ccgaggacgg caacgccgcc agcgattctc
     8941 acctggaagc agaaggtggt cctacctcgc gagcggaccg ccttgggcga gcagatgtgc
     9001 gagcgtccgc tggacgacga gaccggcccg ttcgccgagc tgttcctgcc gcgcgacgtg
     9061 ctgcgccggc tgggcgcccg ccatatcggc cgccgcgtgg tgctgggcag ggaggccgac
     9121 ggctggcgct accagcgtcc gggcaagggg ccgtccacgc tgtacctgga cgccgccagc
     9181 ggcgagccgc tgaggatggt gaccggggat gaggcgtcgc gcgcgtcgct ttgcgaattt
     9241 cccaacgtca gggagggccg agattcccga cgcccgtctt ccccccaagc gctagggcct
     9301 ggatcgggca gaaaaacggg gctgctttcg ggcagccccc ttgtcgtcgc cggacgcgga
     9361 tcaggcggga acggccagcg cagcgggcga gaaggcgcgg cgttgccaga acggctgctc
     9421 cggccagcgg ttttcctgcc ggtaatgccg ccgccgctcc gccagatagc ggcggaagca
     9481 gtcgtcggcg cgcttggcgt aggcgcgagc cggcgccagg tcgccgtcca gcatcgcggc
     9541 gatggccggc gcggcggcca gcgcgtcgcc gagcgcgcag gcgatgccgg acgaggtcag
     9601 tgggtccagc cccatcagcg cgtcgccgac gcagatccag ccggggccgg cggcgcgggc
     9661 gacgcagccg ctgtgcgcgg cgaaggcgtg cgcggcgtcg atccgctgcg gcggcggcag
     9721 cctctccgac agcagccggg tggcgcgcca ggcagccagg aaattggcgg gatgttccag
     9781 ccccagttcc cgggccaggt cctggtccgt catcagcgtg gccagcgcgc ggccgtccgg
     9841 cagcgccgcg gcataccacc agccgtaggg cgcggactcc accagcgagt agccgctcag
     9901 ttgtccgggg ccgtcgacgc ggcacgccag cgcctgcagc cggtccagcc tgcagcggga
     9961 aatgcccagc ctggcggcca gcggcgagcg tctgccgccg gcatccacca ccacccgcgc
    10021 ttccacttcg ccgaggccgc gcacccgcag ccgccagccg ccagcggctg gctcgatggc
    10081 gtcgacgccg gatt
//
