LOCUS       BGC0000831              7315 bp    DNA     linear   BCT 13-JUN-2012
DEFINITION  Duganella sp. B2 violacein biosynthetic gene cluster, complete
            sequence.
ACCESSION   BGC0000831
VERSION     BGC0000831
KEYWORDS    .
SOURCE      Duganella sp. B2
  ORGANISM  Duganella sp. B2
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Massilia group; Duganella; unclassified Duganella.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-07 20:45:02
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     gene            1..1308
                     /gene="vioA"
     CDS             1..1308
                     /codon_start=1
                     /gene="vioA"
                     /product="tryptophan 2-monooxygenase"
                     /protein_id="ACT67682.1"
                     /transl_table=11
                     /translation="MTNYSDICIVGAGIGGLSCATQLINAAAGKNLRIRVFDMDTTVGG
                     RIQSHKVDEEEIAELGAARYSPQLHPHFEQLMQECGLAHATYPFTQVVSLDQAQEKLKA
                     TLLSLSAMLKKHPNDSFLEFVSQYLGAAEATRMIKATGYDALLLPVVSAAMAYDIIKKH
                     PETQSFTENAANEWRYATDGYSELLRQLQRQAQDAGVEFRLGHRLLSVEKSGTDHVLAF
                     RHMGDTQMHRARHVITTLPPTAMKRLNMDFPAAFSPFQYDSLPLFKGFLTFETAWWDAL
                     GLTDKVLMADNPLRKIYFKGDKYLVFYTDSASATYWREYLEQGEDVYLDRVRHHLKEVL
                     PLNGQPLPQIKAHFYKHWPHGVEFSLEPEAEHPATLLHRDGIISCSDAYTSHCGWMEGS
                     LISAGHATRLLLERLSHPPAQSGHDFTLATSLTERA"
     source          1..7315
                     /db_xref="taxon:445969"
                     /mol_type="genomic DNA"
                     /organism="Duganella sp. B2"
                     /strain="B2"
     misc_feature    1..7315
                     /note="violacein biosynthetic gene cluster"
     protocluster    1..7315
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[1304:4322]"
                     /cutoff="20000"
                     /detection_rule="(indsynth or dmat or indole_PTase)"
                     /neighbourhood="10000"
                     /product="indole"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1305..4322
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(indsynth or dmat or indole_PTase)"
                     /neighbourhood="10000"
                     /product="indole"
                     /protocluster_number="1"
     cand_cluster    1..7315
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(indsynth or dmat or indole_PTase)"
                     /kind="single"
                     /product="indole"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..7315
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000831"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..7315
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="indole"
                     /region_number="1"
                     /rules="(indsynth or dmat or indole_PTase)"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            1305..4322
                     /gene="vioB"
     CDS             1305..4322
                     /codon_start=1
                     /gene="vioB"
                     /gene_functions="biosynthetic (rule-based-clusters) indole:
                     indsynth"
                     /gene_kind="biosynthetic"
                     /product="polyketide synthase"
                     /protein_id="ACT67683.1"
                     /sec_met_domain="indsynth (E-value: 0.0, bitscore: 1614.7,
                     seeds: 14, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSILDFPRFHFRGFARANVPTANRNTHGHIDIATNAVSIAGEPFD
                     LSRPPSEFHEHMKQLAPRFDAAGKPDPEGIFSEAAGYNFCGNNHFSWENARITGVQLRD
                     GEVDTEDALVGAKLALWGHYNDYLRTTVNRARWVDNNPAEPDTTLIYAGQFTLSGKQAT
                     PNTPSLFSADIGQAHSVRWVGSGHISERSGHFLDDEFGRSRLFQFSVAKLDPHFLFNPD
                     TPLPASMRALQEALADEDVLGLTVQYALFNMSTPQKPDSPVFYDLAGGIGLWRRDELAT
                     YPAGRLLLPRQSSLGPVLVKVHADRVSVNMPTAIPFTTREAGAVSEQHPTHALGGKLAL
                     GDLMLHGATGKLIARIPQQLYLDYWRHHGVFDVPLLHPATASGSLSMSSAQAQWDEADW
                     VLQSDSNHLYLEAPNRQRQQDFPQTITVQSRLRGELAAHPALTAQAQDGEIVGVRVAPS
                     ALGVGYADLTLTGRRSGATRIMLGEAQGQQFIGARVLPDDWHLDDIPAEQVDYAFLYQH
                     VMSYYELVYPFMSDKVFSLADHCKCETYSRLMWQMCDPQNRDKSYYMPSTRELSLPKSR
                     LFLKYLTQVEAAAKSTLPQAVAPHVIGCKAEWIAELKKAIDLELSLMLQYLYAAYAIPN
                     YAQGVKLVEAGRWLPDELELACGTEDRRRNSGARGALLEIAHEEMIHYLMVNNVLMALG
                     EPFYAGAPALGEQARQRFGLDTEFAFDPFSEHVLARFVRFEWPDYLPTPGKSIATFYAA
                     IRQAVADLPDLFDADGGKRGGEHHLFLKELTNRAYPAYQLEVSDRDSALFALDFVTEQG
                     EGVAVESPHFAVSHFQRLRALAGRFSAREKPFEPAVPALKNPVLDPRADCTVVTDPKAR
                     SLMQLYQGCYELTFALMAHHFAQKPLGSLRRSRLMNASIDIMTGLLRPLSAALMNMPSG
                     VPGRNAGPPVPGPADTAISSDYSLGCELLAQKCLALAQYARSLEAEVASTAQIDMLEFF
                     NQQLTDLSRGKMSREA"
     CDS             4323..5612
                     /codon_start=1
                     /gene="vioC"
                     /gene_functions="other (smcogs) SMCOG1087:hypothetical
                     protein (Score: 148.9; E-value: 4e-45)"
                     /gene_kind="other"
                     /product="VioC"
                     /protein_id="ACT67684.1"
                     /transl_table=11
                     /translation="MHKIIIVGGGLAGSLTATYLAQRGHEVHVIEKRGDPLRAESANAD
                     PVNSRAIGVSMTVRGIKAVLAAGISKEELDRCGEPVVGMAFSVGGGHKVRELTPLEGLF
                     PLSLDRTAFQRLLNKYAVMHKVNYYFEHKCLDLDLDKKSVLIQGPDGALRHLQGDLIIG
                     ADGAHSAVRRAMQAGMRRFQFEQSFFRHGYKTLVLPNAAGLGFRKDLLYFFGMDSKGQF
                     AGRAATIPDGSISFALCLPYTGTPSLATRDRAAMGEFFSRYFGILPPASREELVNQFIA
                     LPSNDLINVRSSTFHYKSNVLLIGDAAHATAPFLGQGMNMALEDVYVFITLLEKHRDDL
                     GLVLSEFTGQRKVQADAMQDMAIANYEMLSNPNFIFFLQTRYTRYMHKKFPSVYPPDMA
                     EKLYFTSVPYDVLQQIQKKQNVWYKLGRVN"
     gene            4323..5912
                     /gene="vioC"
     gene            5612..6730
                     /gene="vioD"
     CDS             5612..6730
                     /codon_start=1
                     /gene="vioD"
                     /product="VioD"
                     /protein_id="ACT67685.1"
                     /transl_table=11
                     /translation="MKILVIGAGPAGLIFASQMKQAQPAWDISVAEKNTQEEVLGWGVV
                     LPGRPPRHPANPLSYLEQPELLNPQFLEEFKLVHHDQPNLMSTGVTLCGVGRQVLVQAL
                     RAKCVAAGITVSYETPLASVAQLEAEYDLVVVSNGINHKSLELPPALAAQVEFGKNKYI
                     WYGTTQLFDQMNLVFRENSNGVFAGHAYKYSDTMSTFIVECSEETYAKAELEFRSERDA
                     AAYIAKTFAPELGEHRLVSQPGQGWRNFMTLSREVASDGKFVLIGDALQSGHFSIGHGT
                     TMAVVVAQLLVKTLSAEAGTAAALASFNARALPLVQLFKEHADASRLWFETVSERVGLG
                     NAELTASFDARRNHLPPLQDALMASLGYALGR"
     gene            6740..7315
                     /gene="vioE"
     CDS             6740..7315
                     /codon_start=1
                     /gene="vioE"
                     /product="VioE"
                     /protein_id="ACT67686.1"
                     /transl_table=11
                     /translation="MPPHATPPLLPMQWSSAYISYWSPMREDDEVTSGYCWFDYARDIC
                     RIDGLFNPWSEKETGHRLWMSEIGDARRGQSRKQKVAYAREAEPAGVKLYERALADEVT
                     PFHELFLPQAILIDGEARHDGRHTVLGQAADAWVVERPGKAASVFYLQAGGNHLLRMVT
                     GNDAQHQSVRDFPNFLAGDIAASVFVSE"
ORIGIN
        1 atgacaaatt attctgacat ttgcatagtt ggcgcaggca tagggggcct cagttgtgcg
       61 acccagttga tcaacgccgc cgccggcaag aatttacgga tcagggtgtt cgacatggat
      121 acgacggtgg gcgggcgcat ccagtcgcat aaagtagacg aggaggaaat cgccgaactc
      181 ggcgccgccc gctactcgcc gcaactccat ccgcatttcg agcaactcat gcaggaatgc
      241 gggctggcgc atgcgaccta ccctttcacc caggtggtgt cgctcgatca ggcgcaggag
      301 aaactgaagg caactctgct gagcctgagt gcgatgctga aaaaacatcc gaacgattcc
      361 ttccttgaat tcgtcagcca gtacctgggc gccgccgaag cgacccgcat gatcaaggcg
      421 accggttacg acgccttgct gctgccggtg gtctcggcag cgatggccta cgacatcatc
      481 aaaaagcacc cggaaacaca aagctttacc gaaaacgccg ccaacgagtg gcgctatgcc
      541 accgacggct acagcgagct gctgcgtcag ttgcagcgcc aggcccagga cgccggcgtg
      601 gaattccggc tggggcatcg cttgctgtcg gttgaaaagt ccggcaccga ccatgtcctc
      661 gccttccgcc atatgggcga tactcagatg caccgggcgc gccatgtgat cacgaccctc
      721 ccgccgaccg ccatgaagcg cctgaacatg gattttccgg ccgccttcag tcccttccag
      781 tacgattccc tgcctttgtt caaaggattc ctgaccttcg aaacagcctg gtgggacgcg
      841 ctcgggctga ccgacaaagt gctgatggcc gataatcctc ttcggaaaat ctacttcaag
      901 ggcgacaaat acctggtgtt ctacaccgac agcgccagcg ccacctattg gcgggagtac
      961 ctggagcaag gggaagacgt ttacctggac cgggtccgcc atcacctgaa agaagtgctg
     1021 ccgctgaacg gtcagccgct gccgcagatc aaggcgcatt tttacaagca ctggccgcat
     1081 ggcgtcgagt tcagcctgga gccggaagcc gagcatccag caaccctgct ccaccgcgac
     1141 ggcatcatct cctgctcgga cgcgtatacc tcgcattgcg gctggatgga aggcagcttg
     1201 atcagcgccg ggcacgctac ccgcctgttg ctggaacggc tcagccatcc gccagcacaa
     1261 tccgggcacg actttaccct cgccacctct cttactgagc gcgcatgagc attctcgact
     1321 ttccccgctt tcattttcgg gggtttgccc gcgctaatgt gcccactgcc aaccgcaata
     1381 cgcacggcca tatcgatatc gcgacgaatg cggtatcgat cgcgggcgaa ccattcgacc
     1441 tgagccgccc gccgtcggaa ttccatgagc acatgaaaca gcttgctccc aggttcgatg
     1501 cggcgggcaa gccggatccg gagggcatct tcagcgaggc ggccggctac aatttttgcg
     1561 gcaacaatca cttttcgtgg gaaaacgcca ggatcaccgg cgtccagctg cgcgatggcg
     1621 aggtcgatac cgaggacgcg ctggtcggcg ccaagctggc cctgtgggga cattacaacg
     1681 actatctgcg caccacggtc aaccgcgcca gatgggtcga caacaacccc gccgagccgg
     1741 acaccaccct catttacgcg ggccagttca cgctcagcgg caagcaagcc acgcccaaca
     1801 caccgagcct gttcagcgcc gatatcgggc aggcgcattc ggtacgctgg gtcggcagcg
     1861 gccatatcag cgagcgcagc gggcattttc tcgacgatga attcggccgt tccaggctgt
     1921 tccagttttc cgtggcgaag ctcgatccgc atttcctctt caatccggac acaccgctgc
     1981 cggccagcat gcgcgccttg caagaggcgc ttgccgacga ggatgtgctg gggctgacgg
     2041 tccagtatgc gctgttcaat atgtcgacgc cgcagaaacc cgattcgccg gtgttctacg
     2101 acctggccgg cggcatcggc ctgtggcgcc gcgacgagct ggccacctat ccggccggcc
     2161 gtttgctgct gccgcgccag agcagcctgg ggccggtact ggtgaaggtg catgcggacc
     2221 gggtgtcggt gaatatgccg acggccatcc cgttcaccac gcgcgaggcc ggcgccgtgt
     2281 ccgaacagca tcctacccat gcgctgggcg gcaagcttgc gctgggcgat ctcatgctgc
     2341 acggtgccac cggcaagctg atcgcccgca tcccgcaaca gctgtacctc gactactggc
     2401 gccatcatgg tgtcttcgat gtgccgctgc tccacccggc cacggcctcg ggttcgctca
     2461 gcatgtccag cgcgcaggcg cagtgggacg aagccgactg ggtgctgcag tccgacagca
     2521 accatctgta tctggaagcg cccaaccggc aacgacagca ggactttccg cagacgatca
     2581 cggtacaaag ccgcttgcgc ggcgagctgg cggcgcatcc ggcgctgacg gcgcaggcgc
     2641 aggatggcga gatcgtcggc gtgcgcgtgg cgccatcggc gctgggagtt ggctatgcgg
     2701 acctgaccct cacgggccgc cgttcaggcg cgacccgcat catgctgggc gaggcacagg
     2761 ggcagcagtt catcggcgcg agggtgctgc ccgacgactg gcatctggac gacataccgg
     2821 ccgaacaggt cgattacgcc tttctctacc agcacgtgat gagctactac gagctggtct
     2881 atcccttcat gtcggacaag gtgttcagcc tggcggacca ctgcaagtgc gaaacctatt
     2941 cgcgcctgat gtggcagatg tgcgatccgc agaaccggga caagagctat tacatgccga
     3001 gcacgcgtga actgtcgctg ccgaaatcgc gcctgttcct gaaatacctg acccaggtcg
     3061 aagcggccgc caagtccacg cttcctcaag cggtcgcacc gcatgtcatc ggctgcaagg
     3121 ccgagtggat cgccgagctg aaaaaggcga tcgacctgga actgtcgctc atgctgcagt
     3181 acctgtacgc cgcgtatgcc attcccaact atgcgcaggg agtgaaactg gtcgaggccg
     3241 gccgctggct gccggacgag ctggagctgg cctgcggcac cgaggaccgg cgccgcaaca
     3301 gcggagcgcg tggcgccctg ctcgaaatcg cccatgaaga aatgattcac tacctgatgg
     3361 tcaacaatgt gctgatggcg ctcggcgagc cgttctacgc cggcgccccg gcgctgggag
     3421 aacaggcgcg ccagcgcttc ggcctggaca cggaatttgc cttcgaccct ttttccgagc
     3481 atgtgctggc cagattcgtg cgtttcgagt ggcccgacta tcttcccacg cccggtaaat
     3541 cgatcgccac cttctatgcg gcgatccgcc aggccgtggc cgatctgccc gatctgttcg
     3601 acgccgacgg cggcaaacgc ggcggcgagc accacctgtt cctcaaagag ctcaccaacc
     3661 gcgcctatcc cgcctatcag ctggaagtta gcgaccgcga cagcgcgctg ttcgcgctcg
     3721 acttcgtcac cgaacagggc gaaggggtgg cggtggaatc gccccatttc gccgtgtcgc
     3781 atttccagcg gctgcgcgcc ctggccggcc gcttttcggc acgcgaaaaa ccgttcgaac
     3841 cggccgtgcc ggcgctgaaa aatccggtac tcgacccacg cgccgactgc accgtggtga
     3901 ccgatcccaa ggcccgttcg ctgatgcagc tctaccaggg ctgctatgaa ctgaccttcg
     3961 cgctgatggc gcaccacttc gcgcaaaagc cgctgggcag cctgcgccgc tcgcgcctga
     4021 tgaatgcctc catcgacatc atgaccggtt tgctgcggcc cttgtcggcc gcgctgatga
     4081 acatgccgtc cggcgtaccc gggcgcaacg ccggaccgcc cgtgcccggg ccggccgaca
     4141 ccgccatcag cagtgactac agcctgggat gcgagctgct ggcgcagaaa tgcctggccc
     4201 tggcgcagta cgcgcgcagc ctggaggccg aggtggccag cacggcgcag atcgacatgt
     4261 tggagttctt taatcagcaa ctgaccgatt tatctcgggg aaagatgtca agagaggcct
     4321 gaatgcacaa aatcatcatc gtgggcggcg gcctggcggg cagcctgacc gccacttatc
     4381 tggcgcagcg cggacatgag gtccacgtta tcgaaaagcg cggcgatccg ctgcgggcgg
     4441 agtcagccaa cgccgatccg gtcaactcgc gcgccattgg cgtgagcatg accgtgcgcg
     4501 gcatcaaggc agtgctggcg gccggcatca gcaaggagga gctcgatcgg tgcggcgaac
     4561 cggtcgtcgg catggcattt tcggtcggcg gcggccacaa ggtgcgcgaa ctgaccccgc
     4621 tcgaaggctt gtttcccttg tcgctggacc gcaccgcgtt ccagcgcctg ctgaacaagt
     4681 acgccgtcat gcacaaagtg aactattact tcgagcataa atgcctggac ctggacctgg
     4741 acaagaaatc cgtgctgatc cagggcccgg acggcgcctt gcggcatctg caaggcgacc
     4801 tgatcatcgg ggccgacggc gcccactccg ccgtgcggcg cgccatgcaa gccggcatgc
     4861 ggcgcttcca gttcgaacaa tccttcttcc gccacggcta caagaccctg gtactgccga
     4921 acgccgcggg actgggcttc cgcaaggatc tgctgtattt cttcggcatg gattccaagg
     4981 gccagtttgc cggccgcgca gccaccatcc cggacggcag catcagcttc gccctctgcc
     5041 tgccctacac cggcacgccc agcctggcca cgcgcgaccg cgccgccatg ggggagtttt
     5101 tcagccgcta cttcggcatc ctgccaccgg ccagccggga agagctggtg aatcagttca
     5161 tcgcgctgcc tagcaacgac ctcatcaatg tccgctccag caccttccat tacaagagca
     5221 atgtcctgct gatcggcgat gcggcgcatg cgaccgcccc cttcctcggt cagggcatga
     5281 acatggcgct ggaagacgtc tacgtcttca tcaccttgct ggaaaagcat cgcgatgacc
     5341 tgggcctggt cttgtcggag tttacggggc agcgcaaggt gcaggccgac gccatgcagg
     5401 atatggcgat cgccaactac gaaatgctga gcaacccgaa ttttattttc ttcctgcaaa
     5461 cccgctacac ccgctacatg cacaagaaat ttcccagtgt ttatccgccc gacatggcgg
     5521 agaagctgta cttcacatcg gttccctacg atgtgctgca acaaatccag aagaagcaaa
     5581 acgtttggta caaacttgga agggtaaact aatgaaaatc ctcgtcatcg gcgccggacc
     5641 agccggactg atctttgcaa gtcaaatgaa gcaagcccaa cccgcttggg atatcagcgt
     5701 tgcggaaaaa aatacccagg aagaagtgct gggctggggc gtggtgctgc cgggacgccc
     5761 gccgcgccat cccgccaatc cgctgtccta tctggagcag ccggaactgc tcaacccgca
     5821 attcctggaa gaattcaagc tggtgcacca cgaccagccg aacctgatga gcaccggcgt
     5881 gacgctgtgc ggcgttggtc ggcaagtcct ggtgcaggcg ctgcgcgcca aatgcgtggc
     5941 ggccggcatc acggtcagtt acgagacgcc gctggcgagc gtggcgcaac tggaagccga
     6001 atacgacctg gtcgtggtgt cgaacggtat caatcacaaa tcgctggaat tgccgccggc
     6061 actggccgcg caggttgaat tcggcaagaa caaatacatc tggtacggca ccacccagct
     6121 gttcgaccag atgaacctgg tgttccgcga aaacagcaac ggcgtgttcg ccggccatgc
     6181 ctacaaatac tcggacacga tgagcacttt catcgtcgag tgcagcgaag agacgtatgc
     6241 caaagccgag ctggaattcc gttccgagcg cgacgccgcc gcctatatcg ccaagacgtt
     6301 cgcgccagaa ctgggcgaac accgtctggt cagccagccg ggccagggct ggcgcaactt
     6361 catgaccctc agccgcgagg tggccagcga cggcaagttc gtgctgatcg gcgatgcgct
     6421 gcaatcgggc cacttctcga tcggccatgg caccaccatg gcggtggtgg tggcccagct
     6481 gctggtgaaa acgctcagcg ccgaagccgg cacggccgcg gccctggcca gcttcaacgc
     6541 ccgcgcgctg ccactggtgc aactgttcaa ggagcatgcc gacgccagcc gcctgtggtt
     6601 cgaaaccgtg agcgagcgtg tcgggctggg caacgcggag ctgaccgcca gcttcgatgc
     6661 ccgccgcaac catttgccgc cgctgcagga tgcgctgatg gccagcctcg gctacgccct
     6721 cggccgctaa ggagacgcca tgccaccgca cgccaccccg ccgctgctgc cgatgcaatg
     6781 gagcagcgcc tacatctcgt attggtcgcc gatgcgggaa gacgacgagg tcacttccgg
     6841 ctattgctgg ttcgactatg cccgcgacat ttgccgcatc gacggcctgt tcaatccctg
     6901 gtcggaaaag gagacgggac accggctctg gatgtcggaa atcggcgacg ccaggcgcgg
     6961 acaaagccgc aaacagaaag tcgcttatgc cagggaggcg gagccggccg gcgtgaagct
     7021 gtacgagcgg gcgctggccg atgaggtcac gcccttccac gagctgttcc tgccgcaggc
     7081 gatcctgatc gacggcgaag cgcgtcatga cggccgccac acggtgctgg gccaggcggc
     7141 cgacgcctgg gtggtggagc ggccgggcaa agccgcctcg gtgttctatc tccaggccgg
     7201 cggcaatcac ttgctgcgca tggtcaccgg caacgacgcg cagcatcagt cggtacgcga
     7261 ctttccgaac ttccttgccg gcgacatcgc ggccagcgtt ttcgtgtcgg aataa
//
