LOCUS       BGC0000851              4523 bp    DNA     linear   BCT 24-JUL-2016
DEFINITION  Streptomyces noursei putative tRNA
            (5-methylaminomethyl-2-thiouridylate)-methyltransferase gene,
            partial cds; albonoursin biosynthetic gene cluster, complete
            sequence; and putative NADP-specific glutamate dehydrogenase gene,
            partial cds.
ACCESSION   BGC0000851
VERSION     BGC0000851
KEYWORDS    .
SOURCE      Streptomyces noursei ATCC 11455
  ORGANISM  Streptomyces noursei ATCC 11455
            Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
            Streptomyces.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-07 20:49:36
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4523
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             <1..228
                     /codon_start=1
                     /note="orf1; similar to Streptomyces coelicolor tRNA
                     (5-methylaminomethyl-2-thiouridylate)-methyltransferase
                     encoded by GenBank Accession Number AL031184"
                     /product="putative tRNA
                     (5-methylaminomethyl-2-thiouridylate)-methyltransferase"
                     /protein_id="AAN07906.1"
                     /transl_table=11
                     /translation="AAARRPTARPSTPPNCAPTAGETEVTAEVVGEELRVSFTEPVRGV
                     APGQAVVLYDGTRVVGSATIATTRRAAPVA"
     source          1..4523
                     /db_xref="ATCC:11455"
                     /db_xref="taxon:316284"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces noursei ATCC 11455"
                     /strain="ATCC 11455"
                     /type_material="type strain of Streptomyces noursei"
     protocluster    1..4523
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[1683:2403]"
                     /cutoff="5000"
                     /detection_rule="(CDPS or cycdipepsynth)"
                     /neighbourhood="10000"
                     /product="CDPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1684..2403
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="(CDPS or cycdipepsynth)"
                     /neighbourhood="10000"
                     /product="CDPS"
                     /protocluster_number="1"
     cand_cluster    1..4523
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(CDPS or cycdipepsynth)"
                     /kind="single"
                     /product="CDPS"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..4523
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000851"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..4523
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="CDPS"
                     /region_number="1"
                     /rules="(CDPS or cycdipepsynth)"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            711..1370
                     /gene="albA"
     CDS             711..1370
                     /codon_start=1
                     /function="sequential conversion of cyclo(L-Phe-L-Leu) into
                     cyclo(dehydro-Phe-L-Leu) and cyclo(dehydro-Phe-dehydro-Leu)
                     (albonoursin)"
                     /gene="albA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00881"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1159:dihydropteridine reductase (Score: 38; E-value:
                     1.9e-11)"
                     /gene_kind="biosynthetic-additional"
                     /note="cyclic dipeptide oxidase; similar to the pfam00881
                     family (nitroreductase family)"
                     /product="AlbA"
                     /protein_id="AAN07907.1"
                     /sec_met_domain="PF00881 (E-value: 5.2e-16, bitscore: 47.8,
                     seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRRHPSHSPYRGGCEVRPKRRGLMLAHSSSESPPESLPDAWTVLK
                     TRTAVRNYAKEPVDDALIEQLLEAMLAAPTASNRQAWSFMVVRRPAAVRRLRAFSPGVL
                     GTPAFFVVACVDRSLTDNLSPKLSQKIYDTSKLCVAMAVENLLLAAHAAGLGGCPVGSF
                     RSDIVTSMLGIPEHIEPMLVVPIGRPATALVPSQRRAKNEVVNYESWGNRAAAPTA"
     misc_feature    783..785
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            1330..1647
                     /gene="albB"
     CDS             1330..1647
                     /codon_start=1
                     /gene="albB"
                     /note="required for AlbA activity"
                     /product="AlbB"
                     /protein_id="AAN07908.1"
                     /transl_table=11
                     /translation="MNPGETVLPPQLREEIALLAVYLLSSGRGLLEEPADYGIYRCTDG
                     ARRALQLLDEHGGSTARLTAVRERLDEVMFAPMGEDRDMGAILDDLCRQMADALPEIET
                     P"
     gene            1684..2403
                     /gene="albC"
     CDS             1684..2403
                     /codon_start=1
                     /gene="albC"
                     /gene_functions="biosynthetic (rule-based-clusters) CDPS:
                     cycdipepsynth"
                     /gene_functions="biosynthetic (rule-based-clusters) CDPS:
                     CDPS"
                     /gene_kind="biosynthetic"
                     /note="sufficient for synthesis of cyclic dipeptides
                     cyclo(L-Phe-L-Leu) and cyclo(L-Phe-L-Phe)"
                     /product="AlbC"
                     /protein_id="AAN07909.1"
                     /sec_met_domain="cycdipepsynth (E-value: 2.5e-84, bitscore:
                     270.9, seeds: 25, tool: rule-based-clusters)"
                     /sec_met_domain="CDPS (E-value: 1.5e-69, bitscore: 222.7,
                     seeds: 17, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLAGLVPAPDHGMREEILGDRSRLIRQRGEHALIGISAGNSYFSQ
                     KNTVMLLQWAGQRFERTDVVYVDTHIDEMLIADGRSAQEAERSVKRTLKDLRRRLRRSL
                     ESVGDHAERFRVRSLSELQETPEYRAVRERTDRAFEEDAEFATACEDMVRAVVMNRPGD
                     GVGISAEHLRAGLNYVLAEAPLFADSPGVFSVPSSVLCYHIDTPITAFLSRRETGFRAA
                     EGQAYVVVRPQELADAA"
     misc_feature    1696..1698
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            2469..3302
                     /gene="albD"
     CDS             2469..3302
                     /codon_start=1
                     /gene="albD"
                     /note="putative membrane protein; similar to Streptomyces
                     coelicolor A3(2) putative membrane protein in GenBank
                     Accession Number CAB45366"
                     /product="AlbD"
                     /protein_id="AAN07910.1"
                     /transl_table=11
                     /translation="MSWGGQDTCSWCGTGPLGEAEDVGRQHTSHAGGPVMTQAATVTAT
                     TSQGRALLRSLTPLFVDAAIPLGSYFLLAEGFGMSTVAALAWSSVVPALRTIWGLVRER
                     TVNGLALLILVVNVVGLATSTLTGDARLMMAKDSGVSSVVGIAILLSVRGRRPLMTAGL
                     RPWVTKGSPEGNAAWDRLWARSARFRQLERRFSTVWGSALLIECVVKVVGAYVLPVHTM
                     VWLGTVLTVVAILLAMVVAGGGSAEPMERMVKAEVGAAGEAATAGNAEPAPAAAA"
     CDS             3401..>4523
                     /codon_start=1
                     /note="orf6; similar to Streptomyces coelicolor A3(2)
                     NADP-specific glutamate dehydrogenase in GenBank Accession
                     Number CAB82051"
                     /product="putative NADP-specific glutamate dehydrogenase"
                     /protein_id="AAN07911.1"
                     /transl_table=11
                     /translation="MRRDIGAPRRRFNGDPDGTRLSSATKGTEVMSTVHTGVTQSGLTA
                     ELASLHAELVRRNPGEAEFHQAALEVLETLAPVLTARPEFADAKVLERIVEPERQIMFR
                     VPWQDDSGTIRVNRGFRVEFNSALGPYKGGLRFHASVNLGIVKFLGFEQIFKNALTGLN
                     IGGGKGGSDFDPHGRSDAEVMRFCQSFMTELHRHLGEHTDVPAGDIGVGGREIGYLFGQ
                     YRRITNRWEAGVLTGKGLAWGGSKARTEATGYGNVLFTEEMLKQRGEELDGQQVVVSGS
                     GNVAIYTIEKAQALGANVLTVSDSGGYVVDEKGIDLALLKQVKEVERGRVGDYAQRRGS
                     SAKYVAGGSVWDVACDVALPSATQNELDADAARI"
ORIGIN
        1 gcggccgccc gccggccgac ggcccggccg tctacaccgc ccaactgcgc gcccacggcc
       61 ggcgagaccg aggtcaccgc cgaggtggtc ggcgaggagc tgcgggtctc cttcactgag
      121 cccgtgcggg gcgtcgcccc gggtcaggcc gtggtcctgt acgacgggac ccgcgtggtg
      181 ggttcggcca ccatcgccac cacccgccgg gccgcgccgg tggcctgagc cgggccgatc
      241 cggccgggcc gtccgcgaca cgccggacgc ggctgagaca tctgacacac cagaggcgtc
      301 tgaggctcgt gtggcgtgtg aggctgtccg aaagtgagcg caccgcaccg cgtcaccacg
      361 acccggccgg gccgggcccg tccctccaag ggcaggcccg gcccggattt ttccaccccg
      421 aaaaccggtg ggaaggtttc cgattttgtt gcgtcgatgg cgtgaattcg gtactgcgga
      481 gcgcttaaat tcacgtcgct gtgacgaacg ctctcccgac cccccttgtc cggccagatc
      541 gcgagctggt acacctagtc cgaaaaatcg ttccgccgaa ctgaatcgaa ctcaagtcgc
      601 cggtcagccg ggcgggtcgg acgtgaatcc ggccccaggg tgatcgatgc gctattgcct
      661 gccgtcggac ccggccgtgc ccgtggatcc gtcccgacgg gcgggaaccg gtgaggcgcc
      721 acccatcgca ttcgccgtac cgcggcgggt gtgaggtgcg cccaaaaaga aggggattga
      781 tgttagctca cagttcatct gaatcgccgc cggaatcctt gccggacgcg tggacggtcc
      841 tcaaaacccg taccgccgtc cgcaattacg cgaaagagcc ggtcgacgac gcgctgatcg
      901 agcagctgtt ggaggccatg ctcgccgcgc cgaccgcctc caaccggcag gcgtggtcgt
      961 tcatggtggt gcgcaggccc gccgcggtcc gccggctgcg cgcgttctcg cccggggtgc
     1021 tgggaacccc cgccttcttc gtcgtggcct gcgtcgaccg cagtctgacc gacaacctct
     1081 ccccgaagct ctcgcagaag atctacgaca ccagcaagct ctgtgtcgcc atggcggtgg
     1141 agaacctgct gctcgcggcg cacgcggccg gcctgggcgg atgcccggtg ggcagcttca
     1201 ggtccgacat cgtcaccagc atgctcggta tcccggaaca catcgagccg atgctcgtgg
     1261 tcccgatcgg ccgtcccgcg acagccctcg tcccctccca gcgccgcgcc aagaatgagg
     1321 tcgtcaacta tgaatcctgg ggaaaccgtg ctgccgcccc aactgcgtga ggagatcgcg
     1381 ctcctcgccg tctatctgct cagcagcggc cgcggactcc tggaggagcc ggccgactac
     1441 ggaatttacc gctgtaccga cggggcccgt cgggcgctcc aactcctcga cgaacacggc
     1501 gggagcacgg cacggctgac cgccgtccgc gagcgtctcg acgaggtcat gttcgcgccg
     1561 atgggcgagg accgggacat gggcgcgatt ctggacgacc tgtgtcgcca aatggcagac
     1621 gctcttccgg aaattgaaac cccctgacgg ctgtccgggg caaccccaaa aggacttctt
     1681 agcatgcttg caggcttagt tcccgcgccg gaccacggaa tgcgggaaga aatacttggc
     1741 gaccgcagcc gattgatccg gcaacgcggt gagcacgccc tcatcggaat cagtgcgggc
     1801 aacagttatt tcagccagaa gaacaccgtc atgctgctgc aatgggccgg gcagcgtttc
     1861 gagcgcaccg atgtcgtcta tgtcgacacc cacatcgacg agatgctgat cgccgacggc
     1921 cgcagcgcgc aggaggccga gcggtcggtc aaacgcacgc tcaaggatct gcggcgcaga
     1981 ctccggcgct cgctggagag cgtgggcgac cacgccgagc ggttccgtgt ccggtccctg
     2041 tccgagctcc aggagacccc tgagtaccgg gccgtacgcg agcgcaccga ccgggccttc
     2101 gaggaggacg ccgaattcgc caccgcctgc gaggacatgg tgcgggccgt ggtgatgaac
     2161 cggcccggtg acggcgtcgg catctccgcg gaacacctgc gggccggtct gaactacgtg
     2221 ctggccgagg ccccgctctt cgcggactcg cccggagtct tctccgtccc ctcctcggtg
     2281 ctctgctacc acatcgacac cccgatcacg gcgttcctgt cccggcgcga gaccggtttc
     2341 cgggcggccg agggacaggc gtacgtcgtc gtcaggcccc aggagctggc cgacgcggcc
     2401 tagttggggg cgtccgcggg cggacctgcc tccccacccg ctcccggtgc cggcgccggg
     2461 catgacaaat gtcatgggga ggacaggaca cttgctcatg gtgcggaacg gggcccctcg
     2521 gcgaagctga agacgtagga agacagcaca cgtcgcacgc cgggggaccc gtcatgactc
     2581 aagccgccac cgtcaccgcc accacgagcc agggcagggc actcctgcgg agcctgacgc
     2641 cgctgttcgt ggacgccgcg atcccgctcg gctcgtactt cctcctcgcc gagggcttcg
     2701 gcatgagcac ggtcgccgcg ctggcctgga gcagcgtggt cccggcgctg cgcacgatct
     2761 ggggcctggt ccgggagcgg acggtcaacg gcctcgcgct gctgatcctc gtcgtcaacg
     2821 tggtggggct ggcgacgagc accctgaccg gcgatgcccg gctgatgatg gccaaggaca
     2881 gcggcgtcag cagcgtcgtc gggatcgcga tcctgctctc ggtgcgcggc cggcgcccgc
     2941 tgatgaccgc cggactccgg ccctgggtga ccaagggaag cccggagggg aacgccgcat
     3001 gggaccggct gtgggcgcgc agcgcgcggt tccggcaact ggagcggcga ttctcgacgg
     3061 tctgggggag cgccctgctg atcgagtgcg tggtcaaggt cgtcggtgcg tacgtcctgc
     3121 cggtgcacac catggtgtgg ctgggcacgg tgctgacggt ggtggcgatc ctgctggcca
     3181 tggtggtcgc gggcggcggc agcgccgagc cgatggagcg gatggtcaag gccgaggtcg
     3241 gggccgccgg cgaggccgcc acggcgggga acgccgagcc ggcgccggcc gccgcggcct
     3301 gagaccgcgc ggcgggggag ttggggaaat cgcgtacagg attggcgcgt cgagcacccc
     3361 cgccctcgat agggcgggcc cccggcgcat atggtcggcg atgcgacggg acatcggagc
     3421 cccgcgtcga cggttcaacg gcgatccgga cggcacgcgg ctttcgtcgg ccacgaaggg
     3481 aacggaagtc atgtcgactg ttcacactgg ggtcacgcag agcggtctca ccgccgagct
     3541 ggcctccctg cacgccgagc tcgtccgtcg gaatcccggt gaagcggagt tccaccaggc
     3601 ggccctggag gtcctcgaaa cgctggcacc ggtgctcacc gcccggccgg agttcgccga
     3661 cgccaaggtc ctggagcgga tcgtcgagcc ggagcggcag atcatgttcc gcgtgccctg
     3721 gcaggacgac tccggcacga tccgggtcaa ccgcggcttc cgggtggagt tcaacagcgc
     3781 gctcggcccc tacaagggcg gcctgcggtt ccacgcgtcc gtcaacctcg gcatcgtgaa
     3841 gttcctcggc ttcgagcaga tcttcaagaa cgccctgacc gggctgaaca tcggcggcgg
     3901 caagggcggc agcgacttcg acccgcacgg caggtcggac gccgaggtga tgcgcttctg
     3961 ccagtccttc atgaccgagc tgcaccgtca cctgggcgag cacaccgacg tgccggctgg
     4021 cgacatcggc gtcggcggcc gggagatcgg ctacctcttc ggccagtacc ggcggatcac
     4081 caaccgctgg gaggccggcg tcctgaccgg caagggcctg gcgtggggcg gctccaaggc
     4141 ccgtacggag gccaccggtt acggcaatgt gctgttcacc gaggagatgc tcaagcagcg
     4201 cggcgaggag ctggacggcc agcaggtggt ggtctccggg tccggcaacg tcgccatcta
     4261 caccatcgag aaggcccagg cgctcggcgc caacgtcctg accgtctcgg actccggcgg
     4321 ctacgtcgtc gacgagaagg gcatcgacct ggcgctgctc aagcaggtca aggaggtcga
     4381 gcgcggccgg gtcggcgact acgcccagcg gcgcggcagt tcggcgaagt acgtcgccgg
     4441 cgggagcgtg tgggacgtcg cctgtgacgt ggcgctgccg tcggccaccc agaacgagct
     4501 cgacgcggac gccgcccgga tcc
//
