LOCUS       BGC0000853              3366 bp    DNA     linear   BCT 06-MAY-2004
DEFINITION  Streptomyces chrysomallus ectoine biosynthesis gene cluster,
            complete sequence.
ACCESSION   BGC0000853
VERSION     BGC0000853
KEYWORDS    .
SOURCE      Streptomyces anulatus
  ORGANISM  Streptomyces anulatus
            Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
            Streptomyces.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-07 20:49:53
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3366
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /db_xref="taxon:1892"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces anulatus"
     protocluster    1..3366
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[2046:2445]"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      2047..2445
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     cand_cluster    1..3366
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..3366
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000853"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..3366
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            9..539
                     /gene="thpA"
     CDS             9..539
                     /codon_start=1
                     /gene="thpA"
                     /note="acyltransferase"
                     /product="ThpA"
                     /protein_id="AAS02094.1"
                     /transl_table=11
                     /translation="MTAAPADFARARSEFLSIDAPRVEDGAAIWRIARDSQVLDLNSSY
                     SYLLWCRDFAATSAVARGENGEPIAFVTGYVRPDRPQTLVVWQVAVDQAHRGKGLAAAL
                     LDALTARVAADQVLSSVETTITPDNTASDRLFTSYAQRHDVALEKEVLFDGELFPEETH
                     LPEVLYRIGPFAT"
     gene            698..1960
                     /gene="thpB"
     CDS             698..1960
                     /NRPS_PKS="Domain: Aminotran_3 (28-363). E-value: 3.4e-70.
                     Score: 228.5. Matches aSDomain:
                     nrpspksdomains_thpB_Aminotran_3.1"
                     /codon_start=1
                     /gene="thpB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013:aminotransferase class-III (Score: 381.1;
                     E-value: 1e-115)"
                     /gene_kind="biosynthetic-additional"
                     /note="aminotransferase"
                     /product="ThpB"
                     /protein_id="AAS02095.1"
                     /sec_met_domain="Aminotran_3 (E-value: 1.7e-78, bitscore:
                     252.1, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITPPALSVFETLESEVRSYCRGWPAVFDRAQGARLTDEDGHSY
                     LDFFAGAGSLNYGHNNPVLKRALIDYIERDGITHGLDMATTAKRAFLETFQNVILRPRD
                     LPYKVMFPGPTGTNAVESALKLARKVKGRESVVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYFDGTVPDFLWFERLLEDQGSGLNKPAAVIVETVQGEGGINVARA
                     EWLRALQELCLRQVMLLIVDDIQMGCGRTGGFFSFEEAGIVPDIVTLSKSISGYGLPMS
                     LCLFKPELDIWEPGEHNGTFRGNNPAFVTAAAVLDAYWADGQMEKQTLARGEQVEQTLL
                     AICAEEPTAQFRGRGLVWGMEFEDKARASAVCARAFELGLLLETSGPQSEVVKLLPPLT
                     ITPEELDEGLRTLARCVRETA"
     aSDomain        782..1786
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_thpB_Aminotran_3.1"
                     /evalue="3.40E-70"
                     /label="thpB_Aminotran_3.1"
                     /locus_tag="thpB"
                     /protein_end="363"
                     /protein_start="28"
                     /score="228.5"
                     /tool="antismash"
                     /translation="FDRAQGARLTDEDGHSYLDFFAGAGSLNYGHNNPVLKRALIDYIE
                     RDGITHGLDMATTAKRAFLETFQNVILRPRDLPYKVMFPGPTGTNAVESALKLARKVKG
                     RESVVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYFDGTVPDFLWFER
                     LLEDQGSGLNKPAAVIVETVQGEGGINVARAEWLRALQELCLRQVMLLIVDDIQMGCGR
                     TGGFFSFEEAGIVPDIVTLSKSISGYGLPMSLCLFKPELDIWEPGEHNGTFRGNNPAFV
                     TAAAVLDAYWADGQMEKQTLARGEQVEQTLLAICAEEPTAQFRGRGLVWGMEFE"
     gene            2047..2445
                     /gene="thpC"
     CDS             2047..2445
                     /codon_start=1
                     /gene="thpC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /note="ectoine cyclase"
                     /product="ThpC"
                     /protein_id="AAS02096.1"
                     /sec_met_domain="ectoine_synt (E-value: 5.1e-57, bitscore:
                     179.4, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFSDIENTDRHVKAASGTWESKRIVLAKEKVGFSLHETVLY
                     AGTETSMWYANHIEAVLCTEGEAELTNDETGETHWITPGTMYLLDGHERHTMRPKTDFR
                     CVCVFNPPVTGREDHDENGVYPLLTEEA"
     gene            2452..3345
                     /gene="thpD"
     CDS             2452..3345
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /note="hydroxylase"
                     /product="ThpD"
                     /protein_id="AAS02097.1"
                     /sec_met_domain="PhyH (E-value: 1e-40, bitscore: 128.4,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTEVRADLYPSRGAAEMTTPRQDPVIWSAPGAPGPVAAKDLQGY
                     EHDGFLTVDQLIAPDEVAVYQAELNRLISDPAVRADERSIVEKQSQNVRSVFEVHRISE
                     VFAGLVRDERVVGRARQILGSDVYVHQSRINVKPGFGATGFYWHSDFETWHAEDGLPNM
                     RTVSVSIALTENFDTNGGLMIMPGSHKTFLGCAGETPKDNYKKSLQMQDAGTPSDEALT
                     KMADRHGIRLFTGRAGSATWFDCNAMHGSGDNITPYARSNVFIVFNSVENAAQEPFAAP
                     IRRPEFIGARDFTPVK"
ORIGIN
        1 caattcgcat gaccgccgca ccagcagatt ttgcccgtgc ccgaagcgaa ttcctcagta
       61 tcgatgcgcc acgagtggag gacggagccg cgatctggcg catagcccgc gactcccagg
      121 tcctggacct gaactcctcg tacagctacc tgctgtggtg ccgtgacttc gccgcgacct
      181 ccgccgtcgc ccgtggcgag aacggcgagc ccatcgcctt cgtgacgggc tacgtcaggc
      241 ccgaccgccc ccagaccctc gtcgtctggc aggtggccgt cgaccaggcc caccgcggca
      301 aggggctggc cgccgctctg ctggacgcac tgaccgcccg ggtcgccgcc gaccaggtcc
      361 tgtcgtccgt ggagacgacg atcacgccgg acaacaccgc ctccgaccgg ctgttcacgt
      421 cctacgccca gcgccacgac gtcgcgctgg agaaggaagt gctcttcgac ggcgagctgt
      481 tccccgaaga gacgcacctg cccgaggtgc tctaccggat aggcccgttc gccacctgag
      541 ccgccccggc caccgcaccc cggcgtaccc gacccgtacg ccggatcccc ctgacttatc
      601 gcgccgaccg accactcggc gcggtcccgg ccgcacccgt gcggcgggga gcactcgacc
      661 actgcccgtc acacccccct cacgcaggag atccgctgtg accatcaccc cgcccgccct
      721 gagcgtcttc gagaccctgg agtccgaggt acggagctac tgccgcggct ggcccgccgt
      781 gttcgaccgc gcgcagggcg cccggctgac cgacgaggac ggccactcct acctggactt
      841 cttcgccggg gccggctcgc tcaactacgg ccacaacaac ccggtgctga aacgagcgct
      901 gatcgactac atcgagcgcg acggcatcac ccacggcctg gacatggcga ccaccgccaa
      961 gcgggcgttc ctggagacgt tccagaacgt gatcctgcgg ccgcgcgatc tgccgtacaa
     1021 ggtgatgttc cccggcccga ccggcaccaa cgccgtcgag tcggcgctga agctggcccg
     1081 caaggtcaag ggccgcgagt cggtcgtctc gttcaccaac gccttccacg gcatgtcgct
     1141 gggctcgctc gcggtgaccg ggaacgcgtt caagcgggcc ggggccggta tcccgctggt
     1201 ccacggcacc ccgatgccgt tcgacaacta cttcgacggc acggtccccg acttcctctg
     1261 gttcgagcgg ctcctcgagg accagggctc cggactcaac aagcccgcag ccgtgatcgt
     1321 ggagaccgtc cagggcgagg gcggcatcaa cgtcgcccgc gccgagtggc tgcgcgcgct
     1381 ccaggagctc tgcctccgcc aggtcatgct gctgatcgtc gacgacatcc agatgggctg
     1441 cggccgcacc ggcggcttct tctccttcga ggaggccggc atcgtcccgg acatcgtcac
     1501 gctctccaag tccatcagcg gctacggcct gcccatgtcc ctctgcctct tcaagcccga
     1561 gctggacatc tgggagcccg gcgagcacaa cggcaccttc cgcggcaaca acccggcctt
     1621 cgtcacggcc gccgccgtgc tcgacgccta ctgggccgac ggccagatgg agaagcagac
     1681 cctcgcccgc ggcgaacagg tcgaacagac cctgctcgcc atctgcgccg aggagccgac
     1741 cgcgcagttc cgcggccggg gcctggtgtg gggcatggag ttcgaggaca aggcgcgcgc
     1801 ctcggcggtc tgcgcccgcg ccttcgagct ggggctcctc ctggagacct cgggcccgca
     1861 gagcgaggtc gtcaagctgc tgccgccgct gaccatcacc cccgaagagc tggacgaggg
     1921 cctgcgcacg ctggcccgct gcgtccgcga aacggcctga cccggcactg ccggggcagg
     1981 cggagaagag aggtcccggg ccgcgaggcc cgggacgctg tacagagaga aaggcaaccg
     2041 ctcaccgtga tcgtccgatc gttcagtgac atcgagaaca ccgaccggca tgtgaaggcg
     2101 gcatccggca cctgggagag caagcgcatc gtgctcgcca aggagaaggt gggcttctcg
     2161 ctccacgaga ccgtgctgta cgcgggcacg gagacctcca tgtggtacgc gaaccacatc
     2221 gaggcggtgc tgtgcaccga gggcgaggcc gagctcacca acgacgagac cggcgagacg
     2281 cactggatca cgccgggcac catgtacctg ctggacgggc atgagcggca caccatgcgg
     2341 cccaagaccg acttccgctg cgtgtgtgtc ttcaaccccc ccgtcacggg acgggaggac
     2401 catgacgaga acggtgtcta cccactgctg accgaggagg cctgaaccac catgaccacc
     2461 gaagtacgcg ccgatctgta cccctcgcgc ggcgccgccg agatgaccac tccccgccag
     2521 gacccggtca tctggtccgc gccgggcgca ccgggtccgg tcgccgccaa ggacctccag
     2581 ggatacgagc acgacggctt cctcaccgtc gaccagctca tcgccccgga cgaggtcgcc
     2641 gtctaccagg cggagctgaa ccggctgatc tccgacccgg cggtccgcgc cgacgagcgc
     2701 tcgatcgtcg agaagcagtc gcagaacgta cggtccgtct tcgaggtcca ccggatcagc
     2761 gaggtcttcg ccggtctggt ccgcgacgag cgggtggtgg gccgggcccg ccagatcctc
     2821 ggctcggacg tgtacgtcca ccagtcccgg atcaacgtga agccgggctt cggggccacg
     2881 ggcttctact ggcactcgga cttcgagacc tggcacgcgg aggacggtct gccgaacatg
     2941 cggacggtgt ccgtgtcgat cgcgctgacc gagaacttcg acaccaacgg cgggctgatg
     3001 atcatgcccg gttcgcacaa gacgttcctc ggctgcgcgg gcgagacgcc gaaggacaac
     3061 tacaagaagt cgctccagat gcaggacgcc ggcaccccgt ccgacgaggc gctgacgaag
     3121 atggccgacc gccacggcat caggctcttc acgggcaggg ccggttcggc gacctggttc
     3181 gactgcaacg ccatgcacgg ctcgggcgac aacatcaccc cgtacgcgcg cagcaacgtc
     3241 ttcatcgtct tcaacagcgt ggagaacgcg gcccaggagc ccttcgcggc tccgatccgc
     3301 cgccccgagt tcatcggggc gcgggacttc accccggtga agtagcgggc cgcacgggcg
     3361 ggaagg
//
