LOCUS       BGC0000855              3558 bp    DNA     linear   BCT 03-JAN-2011
DEFINITION  Methylomicrobium kenyense strain AMO1 ectoine biosynthesis gene
            cluster, complete sequence.
ACCESSION   BGC0000855
VERSION     BGC0000855
KEYWORDS    .
SOURCE      Methylotuvimicrobium kenyense
  ORGANISM  Methylotuvimicrobium kenyense
            Bacteria; Proteobacteria; Gammaproteobacteria; Methylococcales;
            Methylococcaceae; Methylotuvimicrobium.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-07 20:50:10
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /db_xref="taxon:269709"
                     /mol_type="genomic DNA"
                     /note="type strain of Methylomicrobium kenyense"
                     /organism="Methylotuvimicrobium kenyense"
                     /strain="AMO1"
                     /type_material="type strain of Methylomicrobium kenyense"
     misc_feature    1..3558
                     /note="ectoine biosynthesis gene cluster"
     protocluster    1..3558
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[2268:2673]"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      2269..2673
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     cand_cluster    1..3558
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..3558
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000855"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..3558
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            246..740
                     /gene="ectA"
     CDS             246..740
                     /codon_start=1
                     /gene="ectA"
                     /product="L-2,4-diaminobutyric acid acetyl transferase"
                     /protein_id="ABB30170.1"
                     /transl_table=11
                     /translation="MLFPLPMAFTAPEDGMAVHRLIAQCPPLDPNSIYCNLLQCSHFSA
                     TSVAASHQGDLVGFVSGYLVPGRADTLFIWQVAVGESARGQGLASTMVQHLLSRPQCAE
                     VAFIETTINPPNRASWALFERLARQLNAELESSTLFDRDRHFEGQHDTEMLVKIGPLRK
                     D"
     gene            788..2098
                     /gene="ectB"
     CDS             788..2098
                     /NRPS_PKS="Domain: Aminotran_3 (22-358). E-value: 2.8e-77.
                     Score: 251.8. Matches aSDomain:
                     nrpspksdomains_ectB_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013:aminotransferase class-III (Score: 369.1;
                     E-value: 4.6e-112)"
                     /gene_kind="biosynthetic-additional"
                     /product="L-2,4-diaminobutyric acid acetyl transferase"
                     /protein_id="ABB30171.1"
                     /sec_met_domain="Aminotran_3 (E-value: 1.6e-80, bitscore:
                     258.8, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKIFDEIESEVQSYARSFPRIFNKAKGEFLYDEEGNEYIDFLAGA
                     GTLNYGHNNPVFKEKLLDYIHSDGITHGLDLHTKAKGEFLETFNEKILKPRGLEYVMQF
                     TGPTGTNAVEAALKLARNVTGRENVVSFTNGFHGVSLGSLAATGNSHHRGAAGVSLNGT
                     SRMPYDGYLGDDIDTTAYLDKVLSDSSSGVDLPAAVIVETVQGEGGINAASFEWLKNLD
                     QVCKKHGVLLIVDDIQAGCGRTGTYFSFEEVGIYPDIVTLSKSLGGYGLPFAVVLIRPE
                     LDQWKPGEHNGTFRGNNFAFVTAKAAIDHYWSDNRFADQIKQKGEYVSSRLNKIVDLYG
                     EGNFTTRGRGMFQGINCVNGDLAGKITRRAFQLGLVIETSGADDHVVKFLCPLTIAEAS
                     LKKGIDIVEQAIKEVCAKESSIPQEKNYFEADYKLAS"
     aSDomain        854..1861
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ectB_Aminotran_3.1"
                     /evalue="2.80E-77"
                     /label="ectB_Aminotran_3.1"
                     /locus_tag="ectB"
                     /protein_end="358"
                     /protein_start="22"
                     /score="251.8"
                     /tool="antismash"
                     /translation="NKAKGEFLYDEEGNEYIDFLAGAGTLNYGHNNPVFKEKLLDYIHS
                     DGITHGLDLHTKAKGEFLETFNEKILKPRGLEYVMQFTGPTGTNAVEAALKLARNVTGR
                     ENVVSFTNGFHGVSLGSLAATGNSHHRGAAGVSLNGTSRMPYDGYLGDDIDTTAYLDKV
                     LSDSSSGVDLPAAVIVETVQGEGGINAASFEWLKNLDQVCKKHGVLLIVDDIQAGCGRT
                     GTYFSFEEVGIYPDIVTLSKSLGGYGLPFAVVLIRPELDQWKPGEHNGTFRGNNFAFVT
                     AKAAIDHYWSDNRFADQIKQKGEYVSSRLNKIVDLYGEGNFTTRGRGMFQGINCV"
     gene            2269..2673
                     /gene="ectC"
     CDS             2269..2673
                     /codon_start=1
                     /gene="ectC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /product="ectoine synthase"
                     /protein_id="ABB30172.1"
                     /sec_met_domain="ectoine_synt (E-value: 5.3e-50, bitscore:
                     156.8, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKVRRLDQAKQTERRIVSPDGNWESTRMLLKDDNMGFSFHITTIY
                     QGADFQMHYQNHLESVYCISGEGEVETLDDGKVYPIGPGTLYILDRHDKHILRAFKEMT
                     MACVFNPPLNGKEVHNAEGAYELDAEALEG"
     CDS             3000..3455
                     /codon_start=1
                     /product="hypothetical protein"
                     /protein_id="ABB30173.1"
                     /transl_table=11
                     /translation="MSSSSCAWTWPAQRNELLQQILDREHSSVDRGVAYATNTVTYFFY
                     LIAGASTLLVLLGWTSIRDIKERVQSLADKKILKLVHEYEERLAIIEQQLNKEAYSIEK
                     NPRGYRPDARRAVIMVASRAGEQSEQQDRDLRSDFALAPRGLRGADL"
ORIGIN
        1 aacgttaaat tacggccata acaacccggt ctttaaagag aagcttttgg actacatcca
       61 tagcgatggc attacccacg gtttggactt gcataccaaa gccaagggcg aatttctgga
      121 aaccttcaat gaaaaaattc tcaaaccgag aggtttggag tatgtcatgc agtttaccgg
      181 gcctaccgga accaatgcgg tcgaagcggc actgaaattg gcgcgtaacg tgaccggacg
      241 ggaaaatgtt gtttccttta ccaatggctt tcacggcgcc cgaggacggc atggccgttc
      301 atcgtttgat tgcgcaatgt cctccgctgg accccaattc catttattgc aatttattgc
      361 aatgtagtca tttttcggcg acttcggttg ccgcgtccca tcaaggtgat ttggtcggct
      421 tcgtttccgg atatcttgtt cccggcagag cggacacctt attcatttgg caagtcgccg
      481 tcggtgaatc ggctcggggc caaggccttg ccagcacaat ggtgcaacat cttctttcac
      541 ggccccaatg cgccgaggtg gcgttcatcg aaactacgat caatccgccc aatcgggctt
      601 catgggcgct tttcgagcgg ctggcccggc agctcaatgc cgaactcgaa agttcgacgt
      661 tgtttgatcg agaccggcat ttcgaaggac aacatgatac cgaaatgctg gttaagattg
      721 gtccgttacg caaggactga ttgcctatcg atatgcctac cccattccat aaaagaggaa
      781 tacgaatatg aaaatttttg acgagatcga atctgaagta caaagttatg cgcgttcatt
      841 tccgcgcatc ttcaacaaag cgaaaggaga atttctctat gacgaggagg gtaacgaata
      901 tatcgacttt ctcgccggag ccggaacgtt aaattacggc cataacaacc cggtctttaa
      961 agagaagctt ttggactaca tccatagcga tggcattacc cacggcttgg acttgcatac
     1021 caaagccaag ggcgaatttc tggaaacctt caatgaaaaa attctcaaac cgagaggttt
     1081 ggagtatgtc atgcagttta ccgggcctac cggaaccaat gcggtcgaag cggcactgaa
     1141 attggcgcgt aacgtgaccg gacgggaaaa tgttgtttcc tttaccaatg gctttcacgg
     1201 cgtcagcttg ggctctttgg cggcgaccgg caattcgcat caccgaggcg cggcaggcgt
     1261 gagtttaaac ggcaccagta gaatgccgta tgacggttat ttaggcgacg acatcgacac
     1321 gaccgcctat ttagataagg tgctttccga ttccagtagt ggtgtcgatt tgcctgcggc
     1381 tgtcatcgtc gagaccgttc aaggcgaggg cggtattaac gcggcaagtt tcgagtggct
     1441 gaaaaatctg gaccaggtgt gtaaaaagca cggcgtattg ctgattgtcg acgatattca
     1501 agccggctgc ggacgtaccg gaacgtattt cagttttgaa gaggtcggga tttatccgga
     1561 tatcgtgacc ttgtctaaat cgctcggcgg ttacggcttg ccttttgccg tggtgctgat
     1621 ccggccggag cttgatcaat ggaagccggg cgaacacaac ggaacgtttc gcggcaataa
     1681 ttttgccttt gtcacggcca aagccgcaat cgaccattat tggtccgata accgtttcgc
     1741 cgatcaaatc aagcaaaaag gcgagtatgt ttcgagccgc ttgaataaga tcgtcgatct
     1801 atatggcgaa ggtaatttca caacgcgtgg gcgaggcatg tttcaaggca tcaattgcgt
     1861 caatggcgat ttggccggaa aaatcacgcg tcgagcgttt caattgggct tggtgatcga
     1921 aaccagcggt gccgacgatc atgtcgtcaa gtttttgtgt ccgttgacga tagccgaggc
     1981 aagcctcaag aaaggcatcg acatcgtcga gcaagcgatc aaggaagtct gtgccaagga
     2041 gagcagcatt cctcaggaaa aaaattattt tgaggcggac tataaactcg ccagttgatt
     2101 cggcgctcag cgcgctataa gcactcattc actcctgaag cggcgagatc gagcatcgaa
     2161 tcgaatgttt cgattcgggc gatttcagcc gcgcatctgg atggcctcgc gtttaattgc
     2221 gcggattctg ccgtttcagc cttcattcaa caacccgaaa catacatcat gaaagtcaga
     2281 cgattagacc aagccaagca aaccgaacgc cgtatcgttt ctcccgacgg aaactgggaa
     2341 agcacgcgca tgcttttgaa agacgacaat atggggtttt cttttcacat cacgacgatt
     2401 tatcaaggtg cggattttca aatgcattat caaaatcatt tggaatcggt gtattgcatc
     2461 tcgggcgagg gtgaagttga aactcttgac gacggcaagg tttatccgat tggcccggga
     2521 acgctttata tcttagatcg acacgacaaa catatcttgc gcgccttcaa ggaaatgacc
     2581 atggcctgcg tctttaatcc gccgctcaac ggtaaggaag tacacaatgc cgagggggct
     2641 tacgaactcg atgccgaagc gctggagggg taagcgagat catgagtgtc gaaaatgctc
     2701 taggatccgc acaacgcgaa gacctatatc gggcgaaagg ccgaaaatca tgaatcacca
     2761 agatcctgtc gtgtccatgc aagatattcg agccatcgaa ccgaaaccgt tttcggtaca
     2821 ttcggggtaa gccgcttaga tgctcaaaac gttgctgatt ttatggctca tgctgccggt
     2881 agccgttaag gccgaaaccg acgtgcctgc ggaaagcgag ccggagacga tagtcgaaga
     2941 agccttggag gccctggaca aaccgctcta tagtccgttc atcgagcgtt atatattgga
     3001 tgagctcaag cagctgcgcg tggacctggc ccgcgcaaag aaacgaatta ttgcagcaaa
     3061 ttttggatag agaacacagt tcggtcgatc gtggggttgc ctacgccacg aataccgtga
     3121 cttatttctt ttatttgatc gccggtgcca gtacgctttt ggttttgctc ggctggacgt
     3181 cgatcagaga catcaaggag cgcgtgcaat cgctggcgga taagaagata ttgaagctcg
     3241 ttcatgagta cgaggagcgt ctggcgatca tcgagcagca gctcaataag gaggcttact
     3301 cgatcgagaa aaatccgcga ggatatcggc cggacgcaag acgtgcagtc attatggttg
     3361 cgagccgggc aggcgagcag tctgagcaac aagatcgcga tttacgatca gattttgcgc
     3421 ttgcgccccg aggattgcga ggcgctgacc tataaggccg atgcggtgct cgatctaggt
     3481 gagcctcagt gggccgcgaa tttatgccag caagcgttga aaatcgatcc ggaaaacggc
     3541 catgcattct atcaattg
//
