LOCUS       BGC0000859              3305 bp    DNA     linear   BCT 30-DEC-2011
DEFINITION  Methylobacter alcaliphilus ectoine biosynthesis gene cluster,
            complete sequence; and aspartokinase (ask) gene, partial cds.
ACCESSION   BGC0000859
VERSION     BGC0000859
KEYWORDS    .
SOURCE      Methylotuvimicrobium alcaliphilum
  ORGANISM  Methylotuvimicrobium alcaliphilum
            Bacteria; Proteobacteria; Gammaproteobacteria; Methylococcales;
            Methylococcaceae; Methylotuvimicrobium.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-07 20:50:43
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3305
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /db_xref="taxon:271065"
                     /mol_type="genomic DNA"
                     /organism="Methylotuvimicrobium alcaliphilum"
                     /strain="ML1"
     protocluster    1..3305
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[2202:2607]"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      2203..2607
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     cand_cluster    1..3305
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..3305
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000859"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..3305
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            259..777
                     /gene="ectA"
     CDS             259..777
                     /codon_start=1
                     /gene="ectA"
                     /product="L-2,4-diaminobutyric acid acetyltransferase"
                     /protein_id="ACB30330.1"
                     /transl_table=11
                     /translation="MLSDKTASPTITLSQPTAEIGAQVHRLISECPPLDPNSMYCNLLQ
                     SSHFSETAVAAGIGDELVGFVSGYRIPQRPDTLFVWQVAVGEKARGQGLATRMLLAILA
                     RPANQDINRIETTITPNNQASWALFEGLAKKLDTQIGSAVMFDKTRHFADQHETEMLVK
                     VGPFKAVQA"
     misc_feature    259..2607
                     /note="ectoine biosynthesis gene cluster"
     gene            820..2151
                     /gene="ectB"
     CDS             820..2151
                     /NRPS_PKS="Domain: Aminotran_3 (21-358). E-value: 2.9e-79.
                     Score: 258.3. Matches aSDomain:
                     nrpspksdomains_ectB_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013:aminotransferase class-III (Score: 383.8;
                     E-value: 1.6e-116)"
                     /gene_kind="biosynthetic-additional"
                     /product="L-2,4-diaminobutyric acid aminotransferase"
                     /protein_id="ACB30331.1"
                     /sec_met_domain="Aminotran_3 (E-value: 9.2e-86, bitscore:
                     276.0, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKIFEEIESEVRSYARAFPRVFDRAQGAKIYDHEGNEYLDFLAGA
                     GSLNYGHNHPVFKEKLLDYIQNDGITQGLDLHTRAKGEFLESFNEYILKPRNLDYIVMF
                     TGPTGTNAVEAALKIARKKTGRENIISFTNGWHGQTLGSLSVTGNSTHRGGAGIALHGS
                     TRIPYDGYLGDDFDTTNLLDKMLSDSSSGVDKPAAVIVETVQGEGGINAASMTWLKSLS
                     EICKRHDVLLIIDDIQAGCGRTGTFFSFEEAGIYPDIVTLSKSLSGYGLPLAIVLMKPE
                     IDQWSPGEHNGTFRGNNHAFVTAKAAIDHFWKDDNFAKEVQRKGRYIADRVNVIVAKYG
                     EGNFNSHGRGMFRGINCVSGDLAGQITRRCFQKGLIIETSGADDHVVKFLCPLIITDEE
                     LEKGIDILEQAIKEVCDKADTIPEGKDFFEGDYSVSEEVSKAGY"
     aSDomain        883..1893
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ectB_Aminotran_3.1"
                     /evalue="2.90E-79"
                     /label="ectB_Aminotran_3.1"
                     /locus_tag="ectB"
                     /protein_end="358"
                     /protein_start="21"
                     /score="258.3"
                     /tool="antismash"
                     /translation="FDRAQGAKIYDHEGNEYLDFLAGAGSLNYGHNHPVFKEKLLDYIQ
                     NDGITQGLDLHTRAKGEFLESFNEYILKPRNLDYIVMFTGPTGTNAVEAALKIARKKTG
                     RENIISFTNGWHGQTLGSLSVTGNSTHRGGAGIALHGSTRIPYDGYLGDDFDTTNLLDK
                     MLSDSSSGVDKPAAVIVETVQGEGGINAASMTWLKSLSEICKRHDVLLIIDDIQAGCGR
                     TGTFFSFEEAGIYPDIVTLSKSLSGYGLPLAIVLMKPEIDQWSPGEHNGTFRGNNHAFV
                     TAKAAIDHFWKDDNFAKEVQRKGRYIADRVNVIVAKYGEGNFNSHGRGMFRGINCV"
     gene            2203..2607
                     /gene="ectC"
     CDS             2203..2607
                     /codon_start=1
                     /gene="ectC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /product="ectoine synthase"
                     /protein_id="ACB30332.1"
                     /sec_met_domain="ectoine_synt (E-value: 8e-48, bitscore:
                     149.8, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRQLQDAQQTNRRIVSPDGNWESTRMLLKDDGMGYSFHITTIF
                     AGADFRMHYQNHLESVYCISGEGEVETLDDGKKYPITPGTLYNLDQHDRHILRAFKELK
                     LACVFNPPLNGKEVHNAEGAYELEAETVSD"
     gene            2667..>3305
                     /gene="ask"
     CDS             2667..>3305
                     /codon_start=1
                     /gene="ask"
                     /product="aspartokinase"
                     /protein_id="ACB30333.1"
                     /transl_table=11
                     /translation="MTFHTVEKIGGTSMSDYVAVRDNIILKPVHRQDLYRRIFVVSAYG
                     GITDMLLEHKKNGQAGIYGQFANSVNDDGWKASLVALKQEMFAINANIFDTAETIKKAD
                     AFIGERLYDTECCLADLQRLCQHGHFSLDAHLSTVREMLASIGEAHSAWNTVQLLQQDG
                     INAVFVDLTGWQTDKHMTLDERIKKAFAEIDLSRQLPIATGYAHSIDGLM"
ORIGIN
        1 atgcatcatt gatcaccaat cattgaaata gagaccgtcg cgttacaaag ccattactgt
       61 aatttgatat gtcatctaat taatctatag caaatgtacg cgacctggac atttaaatta
      121 tttagtgtac tatatattat gtttttcaat gaagccagaa tattcgtgct gatggccaat
      181 aaaaatcaca ttcaagcccg gcttattcag ttaaagccga attaattgca cagaaatcta
      241 aataatagag ttgtttatat gttgtctgat aaaactgctt cgccgactat tactcttagt
      301 cagcctactg ccgaaatcgg ggcgcaagtg caccgtctga ttagcgagtg tccgccctta
      361 gatcctaatt ctatgtactg taatttgctg cagagcagcc atttttctga aacggcggtg
      421 gcggccggta ttggcgacga gttggttggt tttgtatcgg gttaccgtat tccgcaacgt
      481 ccggatacct tgtttgtttg gcaggtggct gtcggcgaaa aagcccgtgg ccaagggctt
      541 gccacgcgga tgctcttagc cattctggcg cggccggcca atcaggatat taaccgtatc
      601 gagacgacta tcaccccgaa taatcaggcc tcatgggccc tgtttgaggg gctggcaaaa
      661 aaactggaca ctcaaattgg cagtgctgtg atgtttgata agacacgcca ttttgcagat
      721 caacatgaaa ccgaaatgct ggtgaaggtt gggccgttca aagcggttca ggcttaattc
      781 actacactat tatcctatct tgctcaggag agaattagaa tgaaaatctt tgaagaaatt
      841 gaatcagaag ttcgctcgta tgcccgtgct tttccacgcg tgttcgatcg tgcccaaggc
      901 gcaaaaatct atgatcatga aggcaacgaa tatctggact ttctcgctgg tgcaggttcc
      961 ctgaattacg gccacaatca cccggtattt aaggaaaagc tgcttgatta tatccagaat
     1021 gatggcatca cgcagggcct ggacttgcat acccgtgcca agggtgagtt tttggaaagt
     1081 tttaatgagt acatccttaa accgcgaaac ttggattaca ttgtgatgtt taccggcccc
     1141 accggcacca acgcggttga ggctgcactg aagatcgccc gcaaaaaaac cggtcgtgaa
     1201 aacattattt cgttcaccaa tggctggcat ggtcagacgc tggggtcact atcggttacc
     1261 ggcaactcca ctcaccgtgg cggtgccggc attgccttgc atggatcaac ccgtattccc
     1321 tatgacggct atctaggtga tgattttgat accaccaatt tgctcgacaa aatgctgtcg
     1381 gattccagca gtggtgtcga caagcctgca gccgtcatcg ttgaaacggt acagggcgaa
     1441 ggcggtatca acgccgcctc gatgacctgg ttaaaaagct tgtcggaaat ctgcaagcgg
     1501 catgatgtgt tgctgattat tgatgatatc caggccggtt gcggtcgcac cggtactttc
     1561 ttcagctttg aagaagccgg aatttatccg gatattgtga ccttgtcaaa atcgctgagc
     1621 gggtatggtt tgccgttagc cattgttctg atgaagcccg aaatcgatca atggtcgccg
     1681 ggtgagcaca atggtacctt ccgaggcaat aatcatgcct ttgtcaccgc caaggcggct
     1741 attgatcatt tctggaaaga cgacaacttt gccaaagaag ttcagcgcaa aggtcggtat
     1801 attgccgatc gtgtcaatgt cattgtcgcc aaatatggcg agggtaattt caactcgcat
     1861 gggcgaggta tgttccgagg cattaactgt gtcagcggtg atttggccgg tcagattacc
     1921 cgcagatgtt ttcagaaagg tttgattatt gaaaccagtg gcgcagacga tcatgtcgtg
     1981 aagttcctgt gtccgctgat tattacagat gaggagctgg aaaaaggcat cgacattctg
     2041 gagcaagcca ttaaagaggt ctgtgataaa gcagacacaa tcccagaagg aaaagacttt
     2101 tttgaaggcg actactctgt cagtgaagaa gtcagcaaag ccggttactg aagcttgctg
     2161 acccaatcag cctattcaaa aaatctactg aagagaatta aaatgatcgt aagacagtta
     2221 caagacgcac aacaaaccaa cagacgcatc gtttcacctg atggaaactg ggaaagcacc
     2281 cgcatgctgc tgaaggatga cggtatgggc tattcctttc atatcaccac tattttcgcc
     2341 ggtgccgatt ttcgcatgca ttaccagaat catcttgagt cggtgtattg catcagtggt
     2401 gaaggcgaag tggaaacgct ggacgatggc aagaagtatc ctatcacccc aggcacactc
     2461 tataacctgg atcaacacga tcggcatatt ctgcgtgctt tcaaggagtt gaaattagct
     2521 tgtgtcttta acccgccttt gaacggtaag gaagtgcaca acgcagaagg tgcctatgaa
     2581 ctggaagccg aaacagtgag tgactaattt tcaatcatgg cagggcggta taggccctcg
     2641 gcatccacga agtaagagag ggcgttatga cgtttcacac cgttgaaaaa atcggcggca
     2701 catccatgag cgattatgtt gcggtgcgcg acaatatcat tctcaaaccg gtccatcgtc
     2761 aggatctgta tcggcgtatt tttgtggtct ccgcctatgg cggtatcacc gatatgttgc
     2821 tggagcacaa aaaaaacggt caggccggaa tttatggtca gtttgccaat agtgtaaacg
     2881 atgacggctg gaaggcttcg ttggtagctc ttaaacagga aatgtttgca attaatgcca
     2941 acatttttga cactgccgaa accatcaaaa aagccgacgc gtttattggc gaacggcttt
     3001 atgacacaga atgctgtctg gccgatttgc agcgtctttg tcagcatggg catttttcgt
     3061 tggatgcgca tctttctacc gttcgggaga tgctggccag tatcggtgag gcgcatagcg
     3121 catggaacac tgtccagttg ctgcaacaag acggtattaa cgcagtcttt gtcgatctga
     3181 ccggttggca gaccgataaa cacatgacgc tcgacgagcg cattaaaaaa gcttttgctg
     3241 agatcgatct gagccgacaa ctgccaattg ccacgggtta tgcgcacagt attgacgggc
     3301 tgatg
//
