LOCUS       BGC0000863             17116 bp    DNA     linear   BCT 29-APR-2006
DEFINITION  Pseudoalteromonas sp. DS-12 eicosapentaenoic acid synthesis gene
            cluster, complete sequence.
ACCESSION   BGC0000863
VERSION     BGC0000863
KEYWORDS    .
SOURCE      Pseudoalteromonas sp. DS-12
  ORGANISM  Pseudoalteromonas sp. DS-12
            Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales;
            Pseudoalteromonadaceae; Pseudoalteromonas.
COMMENT     ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2022-11-07 20:51:42
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 17116
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..17116
                     /db_xref="taxon:375285"
                     /mol_type="genomic DNA"
                     /organism="Pseudoalteromonas sp. DS-12"
                     /strain="DS-12"
     subregion       1..17116
                     /aStool="mibig"
                     /contig_edge="True"
                     /label="Other"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..17116
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            1225..9507
                     /gene="pfaA"
     CDS             1225..9507
                     /NRPS_PKS="Domain: PKS_KS(Hybrid-KS) (27-480). E-value:
                     6.7e-105. Score: 343.2. Matches aSDomain:
                     nrpspksdomains_pfaA_PKS_KS.1"
                     /NRPS_PKS="Domain: PKS_AT (608-916). E-value: 3.9e-55.
                     Score: 179.1. Matches aSDomain:
                     nrpspksdomains_pfaA_PKS_AT.1"
                     /NRPS_PKS="Domain: PP-binding (1336-1402). E-value:
                     1.5e-12. Score: 39.8. Matches aSDomain:
                     nrpspksdomains_pfaA_PP-binding.1"
                     /NRPS_PKS="Domain: PP-binding (1431-1497). E-value:
                     1.5e-12. Score: 39.8. Matches aSDomain:
                     nrpspksdomains_pfaA_PP-binding.2"
                     /NRPS_PKS="Domain: PP-binding (1526-1592). E-value:
                     1.5e-12. Score: 39.8. Matches aSDomain:
                     nrpspksdomains_pfaA_PP-binding.3"
                     /NRPS_PKS="Domain: PP-binding (1621-1687). E-value:
                     1.5e-12. Score: 39.8. Matches aSDomain:
                     nrpspksdomains_pfaA_PP-binding.4"
                     /NRPS_PKS="Domain: PP-binding (1716-1782). E-value:
                     1.5e-12. Score: 39.8. Matches aSDomain:
                     nrpspksdomains_pfaA_PP-binding.5"
                     /NRPS_PKS="Domain: PKS_KR (2136-2362). E-value: 2.4e-43.
                     Score: 140.0. Matches aSDomain:
                     nrpspksdomains_pfaA_PKS_KR.1"
                     /NRPS_PKS="Domain: PKS_DH2 (2454-2631). E-value: 1.7e-07.
                     Score: 22.7. Matches aSDomain:
                     nrpspksdomains_pfaA_PKS_DH2.1"
                     /codon_start=1
                     /gene="pfaA"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hglE-KS: hglE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PKS_AT"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022:Beta-ketoacyl synthase (Score: 206.8; E-value:
                     9.6e-63)"
                     /gene_kind="biosynthetic"
                     /note="PfaA; similar to CP000083 and SSU73935"
                     /product="polyunsaturated fatty acid synthase"
                     /protein_id="ABF00127.1"
                     /sec_met_domain="PP-binding (E-value: 1.1e-13, bitscore:
                     39.8, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="PKS_AT (E-value: 2.9e-56, bitscore: 179.1,
                     seeds: 1682, tool: rule-based-clusters)"
                     /sec_met_domain="hglE (E-value: 2.8e-224, bitscore: 732.2,
                     seeds: 4, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAHTEPSDQEANRQKDLRLNKRLADMPIAIVGMASIFANGRYLPN
                     FWDNICEKVDAIEDVPCDHWRIEDYYSVDKKGADKSYCKRGGFMPEVDFNPMEFGLPPN
                     ILELTDSSQLLSLIVAKEVLQDANLREDYDRDKIGITLGVGGGQKLSHSLNARLQYPVL
                     DKVFKASGVTDEDSEMIIKKFQDYYVHWEENSFPGSLGNVISGRIANRFDLGGMNCVVD
                     AACAGSLAADRMAITELTEGRSEMMISGGVCTDNSPDMYMSFSKTPAFTTNEQPFDFDS
                     KGMMIGEGIGMRALKRLDDAERDGDRIYAVIKGVGASSDGKFKSIYAPRPEGQAKALVR
                     AYDDAGFAPHSVGLIEAHGTGTAAGDVAEFFGLSSVFSEDNDEKQHIALGSVKSQIGHA
                     KSAAGTAGVIKASLALHHKVLPPTIDIVTQPNPSLEIERSPFYLNTQARPWMIRVDGTP
                     RRAGISSFGFGGTNFHIVLEEYTPEARRGDALKYRQRAVAQILLFTAADRTALLNELND
                     WAQNASNKDTQPEAHNQGAENYGLRSIAKQAARCGFIANDYEELNTMTKQAITQLEAST
                     DGGPINHWQLPSGISYRAAGLVNGKEQTKVAALFAGQGSSQYLNMGRELACYFPELMQQ
                     FHKADQVFTANGKPALSQVLYPIPMFNDESLKAQETILRNTQNAQSAIGVMSVGQFKLF
                     TAAGFNPDMVAGSHFGELSALCAAGVISDDDYIKLAFARGQAMATPSKDGAETDAGFMY
                     AVITKQGDDPEVNGCIAKFEGVSIANYNAPTQVVIAGPTDQTALAADALKEDGFKAIPL
                     PVSGAFHTPLVGHAQKPFADAIDNATFNTPARALYSNGTGGLHKIDANKIKASFKDHML
                     QSVRFNSQLEMYADGARVFVEFGPKNILTKLVEGTLTNQEDEVCTISINANPKGDADMQ
                     LKQAAVQLAVTGISLREIDPYQAVIAPPAAGHMSPMSIKLNATNHISKATRKKMDDSLE
                     DGWVTSQAEHVEREVPEQEVEKIVEKEVIKERIVEVPKVAELPTQGNSVFAGQIQDRSP
                     VNFTAEDWFRTDVLNIGEASLHAFFDAQQQLANLHQQFLAGPKQYGETFSTLMTEQAKM
                     SGIAIPESLDRSMELFHQHQAETLRSHAQYLNMQTGSNAVLGLLNSQTTHPNAVYNSPI
                     QTQVVIKAPTQKPVISPAVINHVSEDWQPAADVNIAQEDGTYAQAEPTVTEVATSEPVK
                     FDGQSALDVTNPQAVMLEVVAEKTGQPTEMLDLEMDMEAPLGIDQIKVEILTSVQDKIA
                     GLPELGPEDLAECRTLTEIVTYMNSKLQATGSNAATPAANLGLSNDKVQATMMAVVAEK
                     TGYPTEMLELEMDMEADLGIDSIKRVEILGTVQDTPGLPELSPEDLAECRTLGEIVDYM
                     FKLPAETGSNAATPAANLGLSNDKVQATMMAVVAEKTGYPTEMLELEMDMEADLGIDSI
                     KRVEILGTVQDTPGLPELSPEDLAECRTLGEIVDYMFKLPAETGSNAATPAANLGLSND
                     KVQATMMAVVAEKTGYPTEMLELEMDMEADLGIDSIKRVEILGTVQDTPGLPELSPEDL
                     AECRTLGEIVDYMFKLPAETGSNAATPAANLGLSNDKVQATMMAVVAEKTGYPTEMLEL
                     EMDMEADLGIDSIKRVEILGTVQDTPGLPELSPEDLAECRTLGEIVDYMFKLPAETGSN
                     AATPAANLGLSNDKVQATMMAVVAEKTGYPTEMLELEMDMEADLGIDSIKRVEILGTVQ
                     DTPGLPELSPEDLAECRTLGEIVDYMFKLPAEAANTSALAANPAIDFTRHKQASCHNMV
                     VADFTYEDGGFIPLKMRVESDVPAAVLNKGAAISTVEPNFDLPPHSEVMLKLSAVNKLV
                     QCFTGSGDKQDYGENCVVIDDGHNAGVIAEKLIAQGWKVAVVRPPKGQHTSAPFNSDVA
                     SYELETHDDFYILTSICAVIKDIETKQGAIDGFIHLDPLQDKTEVTALELHDQAFTSIM
                     AFLWAKLLQPKLNEGDDARRAFVTVSRIDGGFGFSNTDASVQWDGNIDAELNQAALFGL
                     TKTLIHEWPQEDGVFCRAIDIATKLDARHVADAINSELFDQQGHTVEVGLDDAISKNNR
                     NTLVAGNAGTDPHATKHASAELNKTDVILVTGGAKGVTFECAISLAKQCQHHFILAGRS
                     QEDADALPSWAEGKQTNELKPAAIRHLITSGEKPTPKQVDAFVWPVQSSREINHALAAF
                     EVGASAEYISMDVTNSVAITKALQPLDKGGRSNEITGLIHGAGVLADKHIQDKTLAEFT
                     KVYGTKVNGLEALLAATNPSKQIKHVAMFSSAAGFYGNTGQSDYAMSNDILNKAAYQFK
                     ANPEAKVMSFNWGPWDAAMVNPALKKMFTDRGVYVIPLQAGAELLSETGICVQLLVGTS
                     MQGSTNKDIEVASVKKPYAERMLSASDPRCMQVTEDSVRVNRDLDPKAMTFIEDHCING
                     HAVLPTVCAIAQWMREAASVMLGAFHWVKVRDYKLLKGVIFDTPEDNEPQEYTLELTPV
                     EGCDSKVTAQKQALISCEGRPHTQYKATLVADNAPMKDIAPFDTIKLPGMNASEKAITT
                     EDEAKSLYSNGTLFHGPRLQGITEVLRFDQGLIAKCELPKVASTDCGEFPPQLHNGGSQ
                     PDREFAEDYLLQAMLVWARLKTGAASLPSSTIGEFVSYAPMSFGEKAWIELDVIKHTTR
                     PQETSLEANISLYRQNGRLSAVMKSAKVTISKNLNEAFLPKKVADQTSPLASQDSWEAC
                     V"
     misc_feature    1225..16560
                     /note="eicosapentaenoic acid synthesis gene cluster"
     aSDomain        1306..2664
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_pfaA_PKS_KS.1"
                     /domain_subtype="Hybrid-KS"
                     /evalue="6.70E-105"
                     /label="pfaA_PKS_KS.1"
                     /locus_tag="pfaA"
                     /protein_end="480"
                     /protein_start="27"
                     /score="343.2"
                     /tool="antismash"
                     /translation="IAIVGMASIFANGRYLPNFWDNICEKVDAIEDVPCDHWRIEDYYS
                     VDKKGADKSYCKRGGFMPEVDFNPMEFGLPPNILELTDSSQLLSLIVAKEVLQDANLRE
                     DYDRDKIGITLGVGGGQKLSHSLNARLQYPVLDKVFKASGVTDEDSEMIIKKFQDYYVH
                     WEENSFPGSLGNVISGRIANRFDLGGMNCVVDAACAGSLAADRMAITELTEGRSEMMIS
                     GGVCTDNSPDMYMSFSKTPAFTTNEQPFDFDSKGMMIGEGIGMRALKRLDDAERDGDRI
                     YAVIKGVGASSDGKFKSIYAPRPEGQAKALVRAYDDAGFAPHSVGLIEAHGTGTAAGDV
                     AEFFGLSSVFSEDNDEKQHIALGSVKSQIGHAKSAAGTAGVIKASLALHHKVLPPTIDI
                     VTQPNPSLEIERSPFYLNTQARPWMIRVDGTPRRAGISSFGFGGTNFHIVLEEY"
     aSModule        1306..5430
                     /complete
                     /domains="nrpspksdomains_pfaA_PKS_KS.1"
                     /domains="nrpspksdomains_pfaA_PKS_AT.1"
                     /domains="nrpspksdomains_pfaA_PP-binding.1"
                     /locus_tags="pfaA"
                     /tool="antismash"
                     /type="pks"
     CDS_motif       2287..2331
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_pfaA_0001"
                     /evalue="7.20E-03"
                     /label="PKSI-KS_m5"
                     /locus_tag="pfaA"
                     /protein_end="369"
                     /protein_start="354"
                     /score="9.3"
                     /tool="antismash"
                     /translation="IEAHGTGTAAGDVAE"
     aSDomain        3049..3972
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_pfaA_PKS_AT.1"
                     /evalue="3.90E-55"
                     /label="pfaA_PKS_AT.1"
                     /locus_tag="pfaA"
                     /protein_end="916"
                     /protein_start="608"
                     /score="179.1"
                     /tool="antismash"
                     /translation="LFAGQGSSQYLNMGRELACYFPELMQQFHKADQVFTANGKPALSQ
                     VLYPIPMFNDESLKAQETILRNTQNAQSAIGVMSVGQFKLFTAAGFNPDMVAGSHFGEL
                     SALCAAGVISDDDYIKLAFARGQAMATPSKDGAETDAGFMYAVITKQGDDPEVNGCIAK
                     FEGVSIANYNAPTQVVIAGPTDQTALAADALKEDGFKAIPLPVSGAFHTPLVGHAQKPF
                     ADAIDNATFNTPARALYSNGTGGLHKIDANKIKASFKDHMLQSVRFNSQLEMYADGARV
                     FVEFGPKNILTKLVEGTLTNQEDEVCT"
     CDS_motif       3316..3438
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_pfaA_0002"
                     /evalue="4.40E-09"
                     /label="PKSI-AT-M_m3"
                     /locus_tag="pfaA"
                     /protein_end="738"
                     /protein_start="697"
                     /score="28.3"
                     /tool="antismash"
                     /translation="GFNPDMVAGSHFGELSALCAAGVISDDDYIKLAFARGQAMA"
     aSDomain        5233..5430
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_pfaA_PP-binding.1"
                     /evalue="1.50E-12"
                     /label="pfaA_PP-binding.1"
                     /locus_tag="pfaA"
                     /protein_end="1402"
                     /protein_start="1336"
                     /score="39.8"
                     /tool="antismash"
                     /translation="MAVVAEKTGYPTEMLELEMDMEADLGIDSIKRVEILGTVQDTPGL
                     PELSPEDLAECRTLGEIVDYM"
     aSDomain        5518..5715
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_pfaA_PP-binding.2"
                     /evalue="1.50E-12"
                     /label="pfaA_PP-binding.2"
                     /locus_tag="pfaA"
                     /protein_end="1497"
                     /protein_start="1431"
                     /score="39.8"
                     /tool="antismash"
                     /translation="MAVVAEKTGYPTEMLELEMDMEADLGIDSIKRVEILGTVQDTPGL
                     PELSPEDLAECRTLGEIVDYM"
     aSDomain        5803..6000
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_pfaA_PP-binding.3"
                     /evalue="1.50E-12"
                     /label="pfaA_PP-binding.3"
                     /locus_tag="pfaA"
                     /protein_end="1592"
                     /protein_start="1526"
                     /score="39.8"
                     /tool="antismash"
                     /translation="MAVVAEKTGYPTEMLELEMDMEADLGIDSIKRVEILGTVQDTPGL
                     PELSPEDLAECRTLGEIVDYM"
     aSDomain        6088..6285
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_pfaA_PP-binding.4"
                     /evalue="1.50E-12"
                     /label="pfaA_PP-binding.4"
                     /locus_tag="pfaA"
                     /protein_end="1687"
                     /protein_start="1621"
                     /score="39.8"
                     /tool="antismash"
                     /translation="MAVVAEKTGYPTEMLELEMDMEADLGIDSIKRVEILGTVQDTPGL
                     PELSPEDLAECRTLGEIVDYM"
     aSDomain        6373..6570
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_pfaA_PP-binding.5"
                     /evalue="1.50E-12"
                     /label="pfaA_PP-binding.5"
                     /locus_tag="pfaA"
                     /protein_end="1782"
                     /protein_start="1716"
                     /score="39.8"
                     /tool="antismash"
                     /translation="MAVVAEKTGYPTEMLELEMDMEADLGIDSIKRVEILGTVQDTPGL
                     PELSPEDLAECRTLGEIVDYM"
     aSDomain        7633..8310
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_pfaA_PKS_KR.1"
                     /evalue="2.40E-43"
                     /label="pfaA_PKS_KR.1"
                     /locus_tag="pfaA"
                     /protein_end="2362"
                     /protein_start="2136"
                     /score="140.0"
                     /tool="antismash"
                     /translation="VILVTGGAKGVTFECAISLAKQCQHHFILAGRSQEDADALPSWAE
                     GKQTNELKPAAIRHLITSGEKPTPKQVDAFVWPVQSSREINHALAAFEVGASAEYISMD
                     VTNSVAITKALQPLDKGGRSNEITGLIHGAGVLADKHIQDKTLAEFTKVYGTKVNGLEA
                     LLAATNPSKQIKHVAMFSSAAGFYGNTGQSDYAMSNDILNKAAYQFKANPEAKVMSFNW
                     GPWD"
     aSModule        7633..9117
                     /domains="nrpspksdomains_pfaA_PKS_KR.1"
                     /domains="nrpspksdomains_pfaA_PKS_DH2.1"
                     /incomplete
                     /locus_tags="pfaA"
                     /tool="antismash"
                     /type="pks"
     CDS_motif       7636..7731
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_pfaA_0003"
                     /evalue="8.00E-04"
                     /label="PKSI-KR_m1"
                     /locus_tag="pfaA"
                     /protein_end="2169"
                     /protein_start="2137"
                     /score="12.1"
                     /tool="antismash"
                     /translation="ILVTGGAKGVTFECAISLAKQCQHHFILAGRS"
     CDS_motif       8167..8241
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_pfaA_0004"
                     /evalue="1.40E-08"
                     /label="PKSI-KR_m4"
                     /locus_tag="pfaA"
                     /protein_end="2339"
                     /protein_start="2314"
                     /score="27.2"
                     /tool="antismash"
                     /translation="MFSSAAGFYGNTGQSDYAMSNDILN"
     aSDomain        8587..9117
                     /aSDomain="PKS_DH2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_pfaA_PKS_DH2.1"
                     /evalue="1.70E-07"
                     /label="pfaA_PKS_DH2.1"
                     /locus_tag="pfaA"
                     /protein_end="2631"
                     /protein_start="2454"
                     /score="22.7"
                     /tool="antismash"
                     /translation="TFIEDHCINGHAVLPTVCAIAQWMREAASVMLGAFHWVKVRDYKL
                     LKGVIFDTPEDNEPQEYTLELTPVEGCDSKVTAQKQALISCEGRPHTQYKATLVADNAP
                     MKDIAPFDTIKLPGMNASEKAITTEDEAKSLYSNGTLFHGPRLQGITEVLRFDQGLIAK
                     CELPKVASTDCGEF"
     gene            9508..12468
                     /gene="pfaB"
     CDS             9508..12468
                     /NRPS_PKS="Domain: PKS_KS(Hybrid-KS) (28-491). E-value:
                     5.9e-86. Score: 280.8. Matches aSDomain:
                     nrpspksdomains_pfaB_PKS_KS.1"
                     /NRPS_PKS="Domain: PKS_KS(Trans-AT-KS) (515-739). E-value:
                     9.2e-12. Score: 36.4. Matches aSDomain:
                     nrpspksdomains_pfaB_PKS_KS.2"
                     /codon_start=1
                     /gene="pfaB"
                     /gene_functions="biosynthetic (rule-based-clusters) PUFA:
                     PUFA_KS"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022:Beta-ketoacyl synthase (Score: 17.9; E-value:
                     2.1e-05)"
                     /gene_kind="biosynthetic"
                     /note="PfaB; similar to CP000083 and SSU73935"
                     /product="polyunsaturated fatty acid synthase"
                     /protein_id="ABF00128.1"
                     /sec_met_domain="PUFA_KS (E-value: 3.3e-210, bitscore:
                     685.8, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIKPLGTYHFDEWSAPNSQHSKASKMNKIAIVGLANQYPDAQTPE
                     QFWQNLLDKDSRSTITPEKLGANPAKYQGVQGDSDRFYCVKAAYISNFSFDPANGYKLP
                     EDTFAGLDESFLWALDTSRNALIDAGVDWNDAVLERCGVVMGNLSFPTTQSNDLEMPIY
                     HSAVEKALQTKLGVPHFKLSPYNAHRTYGFANISSLNAANGLIAHNPSKLVAQALALGG
                     AQLSLDAACASSCYSLKLACDYLTTGKADMMLAGAVSGADPFFINMGFSIFHAYPDHGV
                     SAPFDANSKGLFAGEGAGVLVLKRLEDAERDGDNIYAVVSGIGLSNDGKGQFVLSPNSK
                     GQVQAFERAYAATNLHPSDIEVIECHATGTPLGDKVELTSMERFFADKLQGSKAPLIGS
                     AKSNLGHLLTAAGMPGIMKMIFAMGEGHLPPSINLKTPISSPKGLFGVNTMPTQVQSWP
                     DKAGNEDWADRRHAGVSVFGFGGCNAHLLLESYTPEADKHSFVAESPKVPRQAEPLKVI
                     GMASHFGSLKNINLHNAITAGTNAFVELPKKRWKGLDKHSNLLQQFGLRSAPKGAYVDQ
                     FEFDFLRFKVPPNEDDRLISQQLMLMKVADAKLEPGQKVAVLVAMETELELHQFRGRVN
                     LHTQIADSLKMGVHLSDDEYQALEAIAMDSVLDAAKLNQYTSFIGNIMASRIASLWDFN
                     GPAFTISAAEQSVYRCIDVAQNLFMKDNLDAVVIAAVDLSGSFEQIILKNAYGPVALHP
                     NVEASEDRTYIVNMLNSTQPQWNVGEGAAAIVLVPTDFVTSGCAGYGQIDALDFGSETV
                     HETPITLDKLLTQAGIAYHFNKVSMIEQCAAPASDNQLEKIPLSTYGPVTHNQVEQRLG
                     HCFAASGMASLLHGLLNLKNRPKQQDTIPCMTEKAKCALINNQSEGQSSALLITQTSAE
                     AQQLTPRLVSDLKSDEKHQLVKQVTLGGRDIADRTILPHNMGFRETLKHNDEAASF"
     aSDomain        9592..10980
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_pfaB_PKS_KS.1"
                     /domain_subtype="Hybrid-KS"
                     /evalue="5.90E-86"
                     /label="pfaB_PKS_KS.1"
                     /locus_tag="pfaB"
                     /protein_end="491"
                     /protein_start="28"
                     /score="280.8"
                     /tool="antismash"
                     /translation="IAIVGLANQYPDAQTPEQFWQNLLDKDSRSTITPEKLGANPAKYQ
                     GVQGDSDRFYCVKAAYISNFSFDPANGYKLPEDTFAGLDESFLWALDTSRNALIDAGVD
                     WNDAVLERCGVVMGNLSFPTTQSNDLEMPIYHSAVEKALQTKLGVPHFKLSPYNAHRTY
                     GFANISSLNAANGLIAHNPSKLVAQALALGGAQLSLDAACASSCYSLKLACDYLTTGKA
                     DMMLAGAVSGADPFFINMGFSIFHAYPDHGVSAPFDANSKGLFAGEGAGVLVLKRLEDA
                     ERDGDNIYAVVSGIGLSNDGKGQFVLSPNSKGQVQAFERAYAATNLHPSDIEVIECHAT
                     GTPLGDKVELTSMERFFADKLQGSKAPLIGSAKSNLGHLLTAAGMPGIMKMIFAMGEGH
                     LPPSINLKTPISSPKGLFGVNTMPTQVQSWPDKAGNEDWADRRHAGVSVFGFGGCNAHL
                     LLESY"
     CDS_motif       10594..10638
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_pfaB_0001"
                     /evalue="1.80E-03"
                     /label="PKSI-KS_m5"
                     /locus_tag="pfaB"
                     /protein_end="377"
                     /protein_start="362"
                     /score="10.9"
                     /tool="antismash"
                     /translation="IECHATGTPLGDKVE"
     CDS_motif       10699..10743
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_pfaB_0002"
                     /evalue="3.20E-02"
                     /label="PKSI-KS_m6"
                     /locus_tag="pfaB"
                     /protein_end="412"
                     /protein_start="397"
                     /score="7.5"
                     /tool="antismash"
                     /translation="GSAKSNLGHLLTAAG"
     aSDomain        11053..11724
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_pfaB_PKS_KS.2"
                     /domain_subtype="Trans-AT-KS"
                     /evalue="9.20E-12"
                     /label="pfaB_PKS_KS.2"
                     /locus_tag="pfaB"
                     /protein_end="739"
                     /protein_start="515"
                     /score="36.4"
                     /tool="antismash"
                     /translation="VIGMASHFGSLKNINLHNAITAGTNAFVELPKKRWKGLDKHSNLL
                     QQFGLRSAPKGAYVDQFEFDFLRFKVPPNEDDRLISQQLMLMKVADAKLEPGQKVAVLV
                     AMETELELHQFRGRVNLHTQIADSLKMGVHLSDDEYQALEAIAMDSVLDAAKLNQYTSF
                     IGNIMASRIASLWDFNGPAFTISAAEQSVYRCIDVAQNLFMKDNLDAVVIAAVDLSGSF
                     EQ"
     CDS_motif       12103..12141
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_pfaB_0003"
                     /evalue="1.60E+01"
                     /label="PKSI-KS_m6"
                     /locus_tag="pfaB"
                     /protein_end="878"
                     /protein_start="865"
                     /score="0.2"
                     /tool="antismash"
                     /translation="VEQRLGHCFAASG"
     CDS             12625..13398
                     /codon_start=1
                     /note="ORF3"
                     /product="unknown"
                     /protein_id="ABF00129.1"
                     /transl_table=11
                     /translation="MRTAKFQQVDVFADKLAQGNPALVVILNHWLSDEMQAISQVNLPE
                     AFILRSMKYGWDYHIRWFTPVGEIKLCGHATLAAGNALFHQNKYHQKEVIFHSSHGKIT
                     KDDQTLRCVFPSWGYEPHAFICPAIEGFLNKAAFEVLTKRGDLMVDLEHEDVSCYQPDF
                     NALAHIDKYRGVIVTAQTTDAGTNYLRCFAPGGISEDPATGSAHCVIAPYWFEKLGKNK
                     LTAIQGLENKGEFCVARGNEVLIYGHAQYLRGVIP"
     gene            13522..16560
                     /gene="pfaD"
     CDS             13522..16560
                     /NRPS_PKS="Domain: ACPS (176-223). E-value: 2.9e-10. Score:
                     31.9. Matches aSDomain: nrpspksdomains_pfaD_ACPS.1"
                     /codon_start=1
                     /gene="pfaD"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1012:4'-phosphopantetheinyl transferase (Score: 214.7;
                     E-value: 1.7e-65)"
                     /gene_kind="biosynthetic-additional"
                     /note="PfaD; similar to CP000083 and SSU73935"
                     /product="polyunsaturated fatty acid synthase"
                     /protein_id="ABF00130.1"
                     /transl_table=11
                     /translation="MATFSQSELAVQKAEKSDGAANIEVFWIPLAEDAGNVWRLGCMWL
                     SSDERAKVQRYKFPKARHNGLIVRAFLRAVLSQHSDERTYIPFGEIRPNEWRFEYGDKG
                     KPRLCHCQFAKTGVHFNLSHSGEWLMVAICTADNKGAKQESKEEDDRNERVCRPQAIEF
                     GDIERERNSTNIHPILSHYFSEAETRTLFALPEALQRQRFFDLWAVKESYIKAKGLGLA
                     LSLKSFAFDIMPSSENFLHPNAPKSHLHCDVDISDFIADHKTGHQLNQRWVLLKQHEGL
                     ALLPKSSGNKPNAEHQKSGAGLIDSKEPQMNAWDVWQCYMGRLDEEYRFAVSIGQPQQI
                     KCIATQEFSIKALVGCSAKTLEDFTNVYKESMILTYGHFREKAVFGTFGDALKNQLGID
                     NGKIVTQAWHVANGIKADCTTQINLLDKNGRLFNAPAGATREQPHYRLAGGQLNFIDKV
                     IVDQGGKEGLGYLYAERTIDPSDWFFQFHFHQDPVMPGSLGVEAIIFLMQTYAISKDLG
                     AGFNNPKFGQILSEVKWKYRGQINPLNKQMSLDVHITSIVDEDGKRIIVGDANLSKDGL
                     RIYEVKDIAICIEEAVPRGGDTLRYDINHFARNGDTLLFFFSYECFVGDKMVLKMDNGC
                     AGFFTDEELSDGKGVIRTEKEIKERSLAVDASTYIGNKQRFNPLLHCPKTQFDYGDIMK
                     LLDADIGCFGSDRTHASHDKGVHQPSLCFSSEKFLMIEQVSHVDPRTGGTWGLGLIEGH
                     KQLEPDHWYFPCHFKGDQVMAGSLMAEGCGQLLQFYMLYLGMHTVQDSETNGRFQPLEN
                     ASQKVRCRGQVLPQSNVLTYRMEVTEIGMSPRPYAKANILLNGKVVVDEQNLGVMIKEE
                     AECTDYPGHSDFESLATQSYVASGAPCQNIADNITPDRAKKISQQKSVNAPLMAQIPDL
                     TKPKNKGVIPIQHFEAPITPGHKYAHRVPDTVPFTPYHMFEFATGNIENCFGPDFSIYR
                     GMIPPRTPCGDTQVTTRVIEVNG"
     aSDomain        14050..14190
                     /aSDomain="ACPS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_pfaD_ACPS.1"
                     /evalue="2.90E-10"
                     /label="pfaD_ACPS.1"
                     /locus_tag="pfaD"
                     /protein_end="223"
                     /protein_start="176"
                     /score="31.9"
                     /tool="antismash"
                     /translation="PILSHYFSEAETRTLFALPEALQRQRFFDLWAVKESYIKAKGLGL
                     AL"
ORIGIN
        1 gatcaggtcg ttgcaggcac agtattaaaa ttaagccgtg ataagatgaa catcacgcga
       61 gaggcggctc ttgcaagtgg gttagcaccc gcgactccat gttatgactt gcaacaggcg
      121 tgcggtacag gattagcggc agctttctta gtagcgaaca aaattgcact agggcaaatc
      181 gagtgcggca tagcgggtgg ttctgacaca atgtctgatg cacccatctc tttcaacgaa
      241 gggatgcgta gcattttgcg ccaggctaac cgatacaaaa ccggcaagga caaacttaag
      301 ccatttatgc aaatccgacc taaagactta atgcacgagt ttccgagaaa cggagaaccc
      361 cgcacaggta tggcaatggg tcaacatgcg gcaataatgg cgcaaacctg gaacatcggt
      421 cgaaaggagc aggacgaatt agcttgtgaa tcacacttga aatacgcagc ttcttacgaa
      481 ggttggttca acgatctggt accgccatac gaaggagcac aagagtatat cctcatcaag
      541 gacaacaatt tgcgccccga cagcacggtt gagaaactag cagggttaaa gccggcattc
      601 gacagaggtg caaacgggac tatgtcagca ggcaattcta ccaacataac cgacggtgca
      661 gtcttaatcg cgagtgaaga gtgggcacag gctcacggat tgaccgtaat ggcgtactgg
      721 agagactcag aggcggcagc tgttgacttt accggtggga aagaaggttt gcttatggca
      781 ccgatatacg cggttaaacg catgttagca cgagcaggcc tcaagttaca agattttgac
      841 tatatcgaaa ttcacgaagc gttcgcagct caagtattat gtaccctcaa ggggtgggag
      901 gacccagaat gcaaaacacg acttgggtta gacagagcgt tgggggactt tgatatgtct
      961 cgcaccaagt tgaatgtata cggttcatct atcgcggctg ggcacccatt cgcggcgagc
     1021 ggaggtcgcg tagtagcgaa tgtcgcacaa atattagacg cgaaagggca gggtcgaatc
     1081 cttgttagta tttgtgcagc tgggggccaa gggatcgcaa tactcgagaa agcggtttag
     1141 cgacagcgca gccgttctcc gggatcaatt cgcggaaagt ctggcatgac gcgccccgac
     1201 aatccatcaa ccagagaaga gacgatggca catactgaac catctgatca ggaggctaat
     1261 cgtcaaaaag atcttcgact taacaaacga cttgcagaca tgccaatcgc gatagttggg
     1321 atggcatcta tttttgcaaa tggtcgctat ttgcccaact tttgggataa catctgcgaa
     1381 aaagttgatg caattgagga tgttccatgc gaccattgga gaatagaaga ctactactca
     1441 gttgacaaga aaggggcaga taaatcttac tgtaagagag gtggatttat gccagaagtt
     1501 gactttaatc ctatggagtt tggtttgcca cccaacatcc tggaactcac agatagctct
     1561 caattgcttt cactcatagt tgctaaggaa gtacttcagg atgctaactt gcgagaagat
     1621 tacgatcgag ataaaattgg catcactcta ggggttggag gtggtcaaaa actatctcat
     1681 agtctgaacg cgcgattgca atacccagtc ctcgataagg tctttaaagc atctggcgta
     1741 accgatgaag attcagagat gattatcaaa aagtttcaag attactatgt acattgggaa
     1801 gaaaacagtt ttccaggttc attgggcaac gttattagcg gcagaattgc aaaccgattt
     1861 gatttggggg gaatgaactg tgttgtcgac gctgcatgcg caggaagcct tgcagctgat
     1921 cgaatggcaa ttacagaatt gaccgaaggt cgttctgaga tgatgatttc tggaggtgtt
     1981 tgcaccgata actctccaga catgtatatg agttttagca aaacccccgc atttacaact
     2041 aatgaacaac catttgactt tgattctaag ggcatgatga ttggagaagg gatcggtatg
     2101 agagctttga aacgactcga tgatgcagaa cgagatggcg atcgcattta cgcagttatt
     2161 aaaggggttg gcgcttctag cgacggtaag tttaaatcta tttacgctcc ccgaccagaa
     2221 ggccaggcaa aagctttggt tagagcttac gatgatgcag ggtttgctcc acatagcgta
     2281 ggcttgattg aagcacacgg taccggcact gctgctggtg acgttgcaga attttttggc
     2341 ctaagcagtg tatttagtga agataatgac gagaagcaac atattgctct cggcagtgtt
     2401 aaatctcaaa ttggccatgc aaagtcagcg gcagggacag cgggtgtcat aaaagcttca
     2461 ttagcgctac atcataaagt tttgccacct acaattgata tcgttaccca gccaaatccc
     2521 agtttggaga tcgaacgatc tcctttttat ctcaacaccc aggctagacc atggatgata
     2581 cgagttgatg ggacaccacg acgagcgggc atatcaagtt ttggttttgg gggaaccaat
     2641 tttcatatag ttttggagga atacacccct gaggctagac gtggggatgc tctaaaatat
     2701 cgacaacgtg cagtagctca aattctacta tttactgcgg ctgatcgtac cgcattgctt
     2761 aatgaactca atgattgggc gcaaaacgct tcaaacaaag atacgcaacc cgaggcacat
     2821 aatcaaggtg cagagaacta cggcttgcga tcaatagcga agcaggcggc gagatgtggg
     2881 tttatagcga acgattatga agaactaaat accatgacta agcaagcgat tacccaactt
     2941 gaggcttcaa ccgatggcgg tcccataaat cactggcaac ttccaagcgg gatttcttac
     3001 cgcgcagcag gtttggttaa cggcaaagag caaacgaagg ttgcagcatt gtttgcaggt
     3061 caaggctcat cacaatattt aaacatgggg cgtgaattgg cttgctactt tcctgaacta
     3121 atgcaacaat ttcataaagc tgatcaagtt tttactgcaa atggtaaacc cgcattaagc
     3181 caagttctct atccaatccc catgtttaac gacgagagtc taaaagcaca agaaaccatt
     3241 ttgcgtaaca ctcaaaacgc tcaatctgca attggtgtta tgagcgtagg ccagtttaaa
     3301 ctatttaccg cagcaggttt taatcccgat atggttgcgg gttctcactt tggagaatta
     3361 agcgcactat gtgcggcagg tgttatttca gatgatgact atataaaatt ggcatttgct
     3421 cgcgggcaag caatggctac cccatcaaag gacggtgcgg aaacagatgc aggatttatg
     3481 tatgctgtaa ttaccaaaca aggcgatgat ccagaggtca acggttgtat cgctaaattc
     3541 gaaggtgttt caattgcaaa ctataatgca ccaacccagg tagtcatagc tggacccact
     3601 gatcagacag cgctcgcagc tgacgcattg aaagaagacg gttttaaggc gattccttta
     3661 ccagttagtg gagcattcca tacccctcta gtcgggcacg cacagaagcc ttttgcagac
     3721 gctattgata acgcaacatt taacacgcca gcgagagcgt tgtactcaaa tggaacaggt
     3781 ggattgcata aaatcgacgc aaataagata aaagctagct tcaaagacca catgttacaa
     3841 tcagtccgtt ttaactcaca gttggaaatg tacgcggacg gggcacgcgt tttcgtcgaa
     3901 tttggtccaa agaacatatt aaccaaactg gtagaaggta ctcttacaaa tcaggaagat
     3961 gaggtttgta cgatcagtat aaatgctaac cctaaaggag acgcggatat gcaattgaag
     4021 caagcagcgg tacaactagc tgttacaggt attagcttga gagagatcga tccctaccaa
     4081 gcagtcattg cgcctccagc agctgggcac atgtctccta tgagcattaa attaaacgca
     4141 acgaatcaca tttcaaaggc tactcgaaaa aaaatggacg actctctaga ggatggatgg
     4201 gttacttcac aggctgaaca tgtcgaacga gaggttccag agcaagaagt agaaaagatt
     4261 gttgaaaaag aggtcattaa agaacgtata gtcgaggtac caaaagtcgc ggagttaccc
     4321 actcaaggaa acagcgtctt tgcaggtcaa attcaggacc gttcaccagt caatttcacg
     4381 gcagaagact ggtttcgcac tgacgtcctt aatattggtg aagcaagttt gcacgcgttc
     4441 tttgatgcac aacaacaatt ggcaaaccta caccagcaat tcttggcagg gcccaaacag
     4501 tatggtgaga cattttctac cttaatgact gaacaagcaa aaatgtctgg aattgcgata
     4561 ccagaaagcc tagacagaag tatggaattg tttcaccagc accaagcaga gactctcaga
     4621 tctcacgcgc aatatttgaa tatgcaaacc ggtagcaacg cagtcttagg gctactcaat
     4681 tctcagacca cacacccaaa cgctgtatac aatagcccta tacaaactca ggttgttatt
     4741 aaggcaccaa cacagaaacc cgtaatatca cctgcagtta taaaccacgt ttcagaggac
     4801 tggcagcctg cggcagatgt taacattgcg caggaggatg ggacatatgc acaagctgaa
     4861 cctacggtta ccgaggttgc tacctcagag cccgttaagt tcgatggtca atcagcattg
     4921 gacgtcacga accctcaagc ggtcatgttg gaagtcgtag cagaaaaaac aggtcaacca
     4981 actgaaatgc tagatttgga gatggatatg gaggcaccct tgggaattga ccagatcaaa
     5041 gttgaaatat taacttcagt tcaggataaa attgcgggac tacctgaact tggaccagag
     5101 gacctagcag aatgtcgaac tttgaccgaa atagttacat atatgaattc taagttacaa
     5161 gctaccggtt ctaacgcagc aacgccagcg gcaaacctag gtttgagtaa tgacaaggtt
     5221 caggcgacga tgatggcagt tgtcgctgag aaaacaggat accctacaga aatgttggag
     5281 cttgaaatgg acatggaggc tgacttgggg atcgattcta taaagagagt tgagattctg
     5341 ggaaccgtcc aagatactcc tggattgcct gaactaagtc cagaggactt ggcggagtgt
     5401 cgtaccctag gagagattgt cgattatatg ttcaaactac ctgcggaaac aggaagtaac
     5461 gctgcaacgc ccgcagcaaa tttagggcta tcaaacgata aagtccaggc aaccatgatg
     5521 gctgtcgtcg ctgagaaaac ggggtacccc acggaaatgt tggaattgga gatggacatg
     5581 gaagcggacc taggaattga ttctattaaa cgcgttgaga tcttgggaac agtacaagac
     5641 acaccagggc tccctgaact aagccccgag gacttagcag aatgtcgaac gctaggagag
     5701 atcgttgatt atatgtttaa attaccagct gaaactggtt ctaacgcggc gacccctgca
     5761 gctaatttgg ggctgagtaa tgacaaggtt caagcaacaa tgatggcagt tgttgctgaa
     5821 aaaacaggtt accccaccga gatgctagaa ttggagatgg acatggaagc ggatttgggg
     5881 atagatagta tcaaacgtgt agagatattg gggacggtcc aagacactcc aggtttacca
     5941 gaactatcac ccgaagattt ggcagaatgt cgtacattgg gagagattgt tgactacatg
     6001 ttcaaattac ccgcggaaac cggtagcaac gcggcaacac cagcagcaaa cttggggtta
     6061 tctaacgata aggttcaagc taccatgatg gcggtcgtcg cagaaaaaac agggtacccc
     6121 accgagatgt tagagctaga aatggatatg gaggcagact tgggtattga ctcaattaaa
     6181 cgcgttgaaa tcctaggaac cgttcaagac acacctggat taccagaatt gtctcccgaa
     6241 gacctggctg aatgtcgtac actaggagaa atcgttgatt atatgtttaa attgccagca
     6301 gaaaccggtt caaacgcggc aacgcctgca gcaaatttag ggttgagcaa cgataaggtc
     6361 caggctacca tgatggcggt cgtcgcggaa aaaaccggat accccacgga gatgctcgag
     6421 cttgaaatgg acatggaggc ggatttaggg attgattcta taaaacgcgt tgaaatttta
     6481 ggtaccgtcc aggacactcc aggtttgccc gaactaagcc cagaggattt ggcagaatgc
     6541 agaacgttgg gggaaattgt agattatatg ttcaaattgc ccgcggaagc agcaaatact
     6601 tcagctctcg cagcgaaccc agcaatagac tttacccgtc acaaacaagc tagctgtcat
     6661 aacatggttg tcgcggactt cacctacgag gatggaggat ttatccctct aaaaatgcga
     6721 gtcgaatcag atgttcccgc ggctgtcttg aacaaagggg cagcaatttc aacggttgag
     6781 ccaaacttcg atttgcctcc acacagtgaa gtcatgttaa aactatctgc agttaacaaa
     6841 ttggtccagt gttttactgg gtctggagat aagcaagact acggggagaa ttgtgttgta
     6901 atcgatgatg gacacaacgc gggtgttata gcagagaaac taatcgctca gggatggaag
     6961 gttgcggtcg tccgtccacc taaggggcag cacacaagtg caccctttaa cagcgacgtt
     7021 gcatcatacg agcttgaaac acacgatgat ttctacatcc taacttctat ttgcgcggtt
     7081 atcaaggata tagagacgaa acagggggca atagacggtt ttatacactt agacccccta
     7141 caagataaaa ccgaagtcac agcgttggaa ttgcatgatc aagcatttac ctctatcatg
     7201 gcattcttgt gggctaaact actacagcca aaattaaacg agggtgacga cgcacgccgt
     7261 gcgtttgtta cagtaagccg aatagacgga ggattcgggt tttcaaacac tgatgcatct
     7321 gtacagtggg atggtaacat cgacgctgag ttgaatcaag cagctttgtt tggtttaacc
     7381 aaaacgctaa tacatgaatg gccacaagag gacggggttt tctgtcgagc aatagacatt
     7441 gcgaccaagc tggatgcacg acacgttgct gatgcaatta actctgagtt gtttgaccaa
     7501 cagggtcaca cagttgaggt agggttagac gacgctatta gtaaaaacaa ccgtaatacc
     7561 ttagtagctg gtaacgcagg tacagatccc cacgcgacaa agcacgcgtc tgcagaattg
     7621 aacaaaaccg atgtcatact agtcaccggg ggggcaaaag gggttacttt cgagtgtgct
     7681 ataagtttag ctaaacaatg ccaacaccat ttcattttag cgggaagatc acaagaagat
     7741 gctgacgcat tacctagttg ggcagaaggg aaacaaacga acgaacttaa gccagcggca
     7801 attcgacact taattacctc aggtgaaaaa cccacgccaa agcaagttga cgcatttgta
     7861 tggcctgttc agagcagccg agaaataaac cacgcattgg cggcgttcga agttggggca
     7921 tcagcggagt acattagcat ggacgttacg aacagcgtcg caataacaaa agcattacaa
     7981 cctttagaca agggagggag aagcaatgaa atcaccgggc taattcacgg tgcgggggta
     8041 cttgcagaca aacatatcca ggacaaaacg ctagctgagt tcaccaaagt ctatggaacc
     8101 aaggtaaacg gtctagaggc acttctggca gcaacgaacc caagcaaaca gattaagcat
     8161 gttgcgatgt tcagtagtgc agcaggtttc tacggtaaca ctggacagtc tgattacgca
     8221 atgtcaaatg atatcttgaa caaagcggca taccaattca aagcaaaccc agaagctaag
     8281 gtaatgtctt ttaattgggg gccatgggat gcagcaatgg ttaacccagc attgaaaaag
     8341 atgttcaccg atagaggtgt atatgttatt cctttacagg caggtgcaga gctcctctca
     8401 gaaacaggta tttgcgtcca attgttagtc ggaacgtcta tgcaagggag tacgaacaaa
     8461 gatattgaag tcgcaagcgt aaaaaagccc tacgcggaac gaatgctgtc agcgtcagat
     8521 ccaagatgta tgcaagttac agaggactca gtacgagtca accgtgatct tgacccaaaa
     8581 gcgatgacct tcattgagga tcactgcatt aacggacatg cggttctacc cactgtctgt
     8641 gcaatagcac agtggatgcg tgaggcagca agcgtaatgt tgggggcttt tcattgggtc
     8701 aaggtacgcg actacaaact gcttaaggga gtcatatttg ataccccaga agataacgag
     8761 cctcaggagt acacattgga attaacccca gtcgaggggt gtgatagtaa agtcaccgca
     8821 caaaagcagg cactaattag ctgtgaaggt cgcccacaca ctcagtataa agcaacattg
     8881 gtagctgaca acgcgcctat gaaggacatc gcgccttttg acaccataaa attgcctgga
     8941 atgaatgcga gcgagaaagc gattaccacg gaagacgagg caaagtctct atacagtaat
     9001 ggaacattgt tccacgggcc ccgactgcaa ggtatcaccg aggtattgcg attcgaccag
     9061 ggtctaatag caaaatgcga gctacctaaa gttgcaagca cagattgtgg agaattccca
     9121 ccccagttgc acaacggagg atcacaacct gatcgtgaat ttgcagagga ctatttgtta
     9181 caagcgatgc tcgtctgggc aagattgaaa acgggggcag cgtcacttcc aagtagtaca
     9241 atcggtgagt tcgtttctta cgcacctatg tctttcgggg aaaaagcatg gatcgagcta
     9301 gacgttataa aacataccac tcgacctcag gaaacaagtt tagaggcaaa catcagctta
     9361 taccgtcaaa atgggcgatt aagtgctgtt atgaagtcag ctaaagtaac gatatctaaa
     9421 aatttgaacg aggcgttcct acccaaaaag gttgcggatc agacctctcc attagcgagt
     9481 caagattctt gggaagcatg tgtatagatg attaaacctc taggtaccta ccatttcgac
     9541 gagtggtcag cacctaatag ccagcactct aaggcgtcaa aaatgaacaa gatagcaatc
     9601 gttggattgg cgaaccaata ccctgatgca cagacccccg aacaattttg gcaaaacttg
     9661 ttagataaag acagccgaag tacgataacc cctgaaaaac ttggtgcaaa ccctgcgaaa
     9721 tatcaggggg tacaaggtga cagtgaccgt ttctactgtg ttaaggcggc atacattagc
     9781 aacttttcat tcgatccagc aaacggttac aagttgcccg aggatacctt tgcagggctg
     9841 gacgagagtt tcttatgggc attggatacc tcacgtaatg cattaatcga tgctggagtt
     9901 gactggaacg atgctgtttt ggagcgttgc ggggtcgtca tgggaaattt gtctttccca
     9961 accacccagt ctaatgactt agaaatgccc atctaccact cagcagtcga aaaagcttta
    10021 caaaccaagc tgggtgtacc acatttcaaa ctctctccat acaacgcaca ccgtacatac
    10081 ggtttcgcga atattagcag cttgaacgca gctaacgggc taatagcaca taaccctagt
    10141 aaattggtcg ctcaagcgtt agctttagga ggggcacagc tcagtctgga cgcggcatgt
    10201 gcgtcttcat gctatagtct aaagctagca tgtgactact tgactacagg taaagcagat
    10261 atgatgttag caggagcggt ttcaggggct gatccattct ttatcaacat ggggttcagc
    10321 atattccatg cttaccccga ccatggagtc tctgcacctt tcgacgcaaa cagtaaaggt
    10381 ttgtttgcag gtgagggggc aggagtctta gtcttaaaac gacttgagga cgctgaacgt
    10441 gatggagaca atatctatgc tgtagtttca ggcattggct taagcaacga tggcaaaggt
    10501 caattcgtcc taagtccaaa cagcaagggc caggtacaag ctttcgagcg cgcatatgcg
    10561 gcaaccaacc tacatccttc agatattgag gtaattgaat gtcacgcaac cgggactcct
    10621 ttgggagaca aagtagagtt aacgtcaatg gaaagattct tcgcggataa actccagggg
    10681 agtaaagcac cattgatagg ttctgctaag agcaacttag gccacttgct tacagcggcg
    10741 ggaatgcccg gtatcatgaa gatgattttc gcgatgggtg aagggcacct acctccatca
    10801 ataaacttga aaaccccaat cagctcacca aagggtctat tcggagtaaa cacaatgcca
    10861 acgcaagtcc aatcatggcc tgacaaagca ggcaacgaag attgggcaga tcgcagacat
    10921 gctggggtct ctgttttcgg attcgggggt tgcaatgcac acttgttact ggagagctac
    10981 actccagaag cagacaagca ctcatttgtc gctgaaagtc ccaaggtccc acgccaagct
    11041 gagcctctaa aggttattgg aatggcatct catttcggtt cattgaaaaa tattaaccta
    11101 cacaacgcga tcacagcagg tacgaacgcg tttgtcgaac ttccaaaaaa gcgttggaag
    11161 gggctggata aacactcaaa cttattgcaa cagtttggac tacgctcagc acctaaaggg
    11221 gcatatgtag atcaattcga atttgatttc cttagattca aagtaccccc aaatgaggac
    11281 gatcgactaa tttctcaaca attgatgctt atgaaggtcg cggatgcaaa attagaaccc
    11341 ggtcaaaaag tcgcagtatt ggtcgcgatg gagaccgaat tagagctcca tcagttcaga
    11401 gggcgagtta acttacatac gcaaattgca gattctttga aaatgggcgt acacttaagt
    11461 gatgatgaat accaagcgtt ggaggcaatt gctatggact ctgttctcga cgcagctaag
    11521 ttaaaccagt acaccagttt tataggtaat atcatggcgt cacgcattgc ttctctatgg
    11581 gatttcaacg gcccagcatt cacgattagc gctgctgaac agtcagttta ccgttgcatc
    11641 gacgtagcgc aaaatttgtt catgaaagat aacctagacg cagttgtcat tgcagcggtc
    11701 gatctttctg gttctttcga gcaaatcatt ttaaaaaacg cttatggacc agttgcacta
    11761 caccctaacg tagaggcgag cgaggatcga acctacatag ttaatatgtt aaactctacg
    11821 cagcctcagt ggaacgttgg ggaaggagcg gcagcaattg tattggtccc tacagatttc
    11881 gttaccagtg gctgtgcggg ctacggacaa atcgatgcat tagactttgg ttcagaaacc
    11941 gtccacgaga caccaattac tcttgataag ttattgacac aagcaggtat tgcttatcac
    12001 ttcaataagg tctcaatgat tgaacagtgt gctgcgcccg catcagacaa ccaattagag
    12061 aaaataccac tatcaacgta cggtcccgtt acgcacaatc aggtagagca acgattgggg
    12121 cattgtttcg cggctagcgg aatggcttca ttattgcatg gtttgttaaa tctcaagaac
    12181 agaccaaaac agcaagacac cattccttgc atgacagaga aagcgaaatg cgctttaatt
    12241 aacaatcaga gcgaaggcca gtcttcagcg ctattgatca ctcaaaccag tgctgaggcg
    12301 cagcagttga cacctagact ggtttctgat ctaaaaagcg atgaaaagca ccagttagta
    12361 aagcaggtaa cactcggcgg gagagatatc gcagaccgaa ctattttgcc ccataacatg
    12421 ggattccgag agaccctgaa gcataacgat gaagcggctt cattctgaag tccgtcagtg
    12481 gtcgtaagtc gtgtggactg tacaactgtg ggtcccaaac tgtgtaactg gtcgtacttt
    12541 gtcatgacaa attgccgact ttgccaaatc gtggtaattt cggaagcctt aaggtacgcc
    12601 tggaactggt caccgagaca acgtatgcgc actgcgaaat tccagcaagt cgatgtattt
    12661 gcagacaaat tggcacaagg aaaccccgct cttgtcgtca ttttaaacca ttggctaagt
    12721 gatgaaatgc aagcgataag tcaagtaaat ttgcccgagg catttatcct ccgatcaatg
    12781 aagtacgggt gggactatca tatccgttgg ttcaccccag taggcgaaat caaattatgt
    12841 gggcatgcaa cgcttgcagc tggtaacgca ttgttccatc agaataaata ccatcaaaaa
    12901 gaggtaattt ttcacagcag tcatggtaaa atcacaaagg acgaccagac gctacgctgt
    12961 gtctttccct cttgggggta cgagcctcat gcgttcatct gtccggcaat tgaaggtttt
    13021 ctaaacaaag cagctttcga ggtcttaaca aaacgagggg atttaatggt tgaccttgag
    13081 cacgaagatg tttcttgtta ccaacccgac ttcaatgcgt tagcacatat tgataagtac
    13141 cgcggggtca tcgtcaccgc acagacgacc gacgctggca ctaactactt acgttgtttc
    13201 gcaccggggg gaatatcaga ggaccctgct accggctctg cacattgtgt tatcgcaccc
    13261 tattggttcg agaaattagg taaaaacaag cttacggcta ttcaggggtt agagaacaag
    13321 ggcgagttct gtgtagcgag aggaaatgag gtcttgatat atggtcacgc gcagtacttg
    13381 cgcggggtaa tcccatgacc taacgtcaaa tgcccgaacg gtaaacccgg taacgtcaat
    13441 gttgacgtcc caaagggtca gggtaagttc ccggtccgaa agtccagtcc atgggtgaca
    13501 atagccgacc cggagcagtc gatggcaacc ttcagccagt cagaattggc agtccagaag
    13561 gctgaaaaaa gcgacggcgc ggcaaacata gaggtctttt ggattccttt agctgaagac
    13621 gcggggaatg tatggcgtct aggctgcatg tggctgagta gtgacgaaag agcaaaagtt
    13681 caacgataca agtttccaaa ggcgagacat aatggtttga tcgtacgcgc attcttacgt
    13741 gcggttttgt ctcaacattc agatgagcgt acctacatcc ccttcggtga aattcgccca
    13801 aatgaatgga gatttgaata cggtgacaag gggaaacccc gtttatgtca ttgccagttt
    13861 gcgaaaacag gagtacactt caatttatct catagtgggg agtggcttat ggttgctatc
    13921 tgcacggcag acaacaaggg agctaagcaa gagagtaaag aggaggacga tcgtaacgag
    13981 cgagtatgtc gccctcaagc gatcgagttc ggagatattg aacgagaacg caactctact
    14041 aacatccatc caattctatc acactatttt agtgaagctg agaccagaac gttgttcgcg
    14101 ttaccagaag cgttacagcg acaacgtttc tttgatctat gggctgtaaa ggagtcttat
    14161 ataaaagcta agggtttggg gttagcacta agcctgaagt catttgcgtt cgatatcatg
    14221 cccagtagtg aaaacttctt gcaccctaac gcgccaaagt ctcacttgca ttgtgacgtt
    14281 gatatcagcg actttatagc tgaccataaa accggtcatc agttaaacca aagatgggtc
    14341 ttactcaaac agcatgaagg attagcgctc ttacccaaga gcagcggtaa caaacccaat
    14401 gctgagcatc aaaaaagcgg agctggccta attgattcta aagagccaca aatgaacgct
    14461 tgggacgtat ggcaatgtta catggggcga ttagatgaag aataccgctt cgcagtatct
    14521 ataggtcaac cccagcagat taagtgcatt gcaacccagg aattttcaat caaggcgtta
    14581 gtcggttgca gcgcaaaaac tcttgaagat tttaccaatg tatacaagga gagtatgatc
    14641 ttgacctacg gtcatttcag agagaaagcg gttttcggca ctttcggtga tgcattaaaa
    14701 aaccaactag gtatcgataa cggcaagata gtcacgcagg cgtggcacgt tgcaaacggg
    14761 ataaaggcgg actgtacgac gcagatcaac ttattggata agaacgggcg tttgtttaat
    14821 gcaccagctg gcgcgacccg agaacagccc cattatcgct tagcaggtgg ccaattaaac
    14881 ttcatcgaca aagttatagt agatcagggg ggcaaagaag gcctgggata cttgtatgcg
    14941 gagagaacca ttgatccctc tgactggttc ttccaattcc acttccatca ggatccagtc
    15001 atgcctggga gcttaggcgt tgaggcgata atttttctaa tgcaaaccta tgcgataagt
    15061 aaagacttag gtgcagggtt caacaatcct aagttcggcc aaatattgtc tgaggttaag
    15121 tggaagtatc gaggacagat caacccttta aataaacaaa tgtcactgga cgttcatatc
    15181 acaagcattg tcgatgaaga tggtaaaaga ataattgtag gcgacgcaaa cttgtctaag
    15241 gacggactcc gaatctatga ggtaaaagac atcgcgatct gtattgaaga agcagtccca
    15301 cgaggtgggg acaccttacg ctacgatatt aaccacttcg cgcgaaacgg tgatacacta
    15361 ctcttctttt tcagctacga atgcttcgtt ggagataaaa tggttttaaa gatggacaac
    15421 ggttgtgcag ggtttttcac ggacgaggaa ctgtctgatg gcaaaggagt catccgtact
    15481 gaaaaggaaa ttaaggaaag atcattagcg gtagacgcta gtacgtatat cggaaacaaa
    15541 cagcgcttca accccttgtt acactgccct aaaacccagt ttgattatgg ggacatcatg
    15601 aagttattgg atgctgacat tggctgtttc ggttctgacc gaactcacgc gtcacatgac
    15661 aagggggtcc accagccatc actatgtttc agtagcgaga aatttttgat gattgaacaa
    15721 gtctcacatg tagatccgcg cactggtggc acgtggggat tgggcttaat cgagggccat
    15781 aagcagctcg aaccagatca ttggtacttc ccatgccact tcaaggggga ccaggtcatg
    15841 gctgggtctt taatggcaga ggggtgtggc caactcctgc agttttacat gttgtatcta
    15901 ggtatgcata ccgtccagga cagcgaaact aacgggcgat tccaaccgtt ggaaaacgcg
    15961 agtcagaaag ttcgttgtag agggcaggtt ctgcctcagt caaacgtcct aacctacaga
    16021 atggaggtaa cggagatagg tatgagtcct cgcccatacg caaaggcaaa catcttgctc
    16081 aacggcaaag tagttgtaga tgagcaaaat ttaggggtaa tgatcaagga agaggcagaa
    16141 tgtaccgatt accccggtca ctcagacttt gaaagcttgg cgacacaatc ttacgtagcg
    16201 agcggcgcgc cttgtcaaaa catagcggat aatatcacgc ctgaccgtgc aaaaaagatc
    16261 tcacagcaga agagcgtcaa tgcaccgttg atggcgcaga tcccagactt aacgaagcct
    16321 aaaaataagg gcgtcattcc tatccagcat tttgaagcgc ctatcacccc gggacacaag
    16381 tacgcacata gagtacccga cacggtccct ttcaccccgt atcatatgtt cgaatttgcg
    16441 accggtaaca tagaaaactg tttcgggccg gatttctcta tctaccgtgg gatgattcca
    16501 ccgcgcaccc cctgcggtga cacacaggta actactcgcg taatcgaagt caatgggtaa
    16561 actggtaccg tttgtccagt tgccacgtca agcctttgcc gtaaacggtt tccgtcagtg
    16621 agatcggtac ggatgaaaat cttgtacccc tgtggaaaga caattttaat caccggcgca
    16681 agcagtgggt tgggtgaagg gatggcacgt gagtttgctg cactaggata caatttagcg
    16741 atatgcgcgc gaagaatgga ccgcttagag tcattaaaaa ccgagctgga agcgcaatat
    16801 ccacacgtaa agatcgaaaa caaaacgcta gacgtcaacg actacgaaca aatttttgaa
    16861 gtattcaacg cttttaagca agatttcggg cacttagacc gtgtaattgt aaacgcggga
    16921 atcggtaaaa gccgacgttt aggcaaggga cacttcgacg caaatatcca gacggcagaa
    16981 accaacttta tcgctgcatt ggcgcagtgt gaagcagcag tcattttccg aaaacagaac
    17041 tcaggtcact tggttgtaat cagcctcatg cttgcagtcc gagggttacc ccgacacatg
    17101 acgaccggga tcggta
//
