BGC0000875

BGC0000875: caprazamycin biosynthetic gene cluster from Streptomyces sp. MK730-62F2

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Location: 1 - 28,072 nt. (total: 28,072 nt).
This entry is originally from NCBI GenBank FJ490409.1.

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

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Gene details

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General

Compounds

Genes

Saccharide

History

KnownClusterBlast

General information about the BGC

MIBiG accession	BGC0000875
Short description	caprazamycin biosynthetic gene cluster from Streptomyces sp. MK730-62F2
Status	Minimal annotation: no A minimal annotation only contains information on the BGC loci and one or more linked chemical product(s) Completeness: complete Whether the loci encodes everything needed for the pathway producing the compound(s)
Remarks	"Caprazamycins are a mixture of derivatives containing different fatty acyl chain lengths, saturation and branching. Non-glycosylated and sulfated derivatives can be found in the extracts too."
Biosynthetic class(es)	Other (Nucleoside) Saccharide (hybrid/tailoring)
Loci	NCBI GenBank: FJ490409.1
Compounds	caprazamycin caprazamycin aglycon
Species	Streptomyces sp. MK730-62F2 [taxonomy]
References	Identification and manipulation of the caprazamycin gene cluster lead to new simplified liponucleoside antibiotics and give insights into the biosynthetic pathway. Kaysser L et al., J Biol Chem (2009) PMID:19351877 Analysis of the liposidomycin gene cluster leads to the identification of new caprazamycin derivatives. Kaysser L et al., Chembiochem (2010) PMID:20039253 A new arylsulfate sulfotransferase involved in liponucleoside antibiotic biosynthesis in streptomycetes. Kaysser L et al., J Biol Chem (2010) PMID:20157116 Formation and attachment of the deoxysugar moiety and assembly of the gene cluster for caprazamycin biosynthesis. Kaysser L et al., Appl Environ Microbiol (2010) PMID:20418426 Heterologous expression of the biosynthetic gene clusters of coumermycin A(1), clorobiocin and caprazamycins in genetically modified Streptomyces coelicolor strains. Flinspach K et al., Biopolymers (2010) PMID:20578003 Caprazamycins, novel lipo-nucleoside antibiotics, from Streptomyces sp. II. Structure elucidation of caprazamycins. Igarashi M et al., J Antibiot (Tokyo) (2005) PMID:16060385 A two-step sulfation in antibiotic biosynthesis requires a type III polyketide synthase. Tang X et al., Nat Chem Biol (2013) PMID:23912167

Chemical products information

caprazamycin

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C₅₄H₈₉N₅O₂₂

Chemical database entries
PubCHEM

caprazamycin aglycon

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C₄₅H₇₃N₅O₁₈

List of genes involved in compound(s) production

Identifiers	Position	Product	Functions	Evidence	Extra
ACQ63617.1 cpz9	103 - 1149 (+)	AraC family transcriptional regulator	Regulation	Sequence-based prediction	copy AA seq copy Nt seq
ACQ63618.1 cpz10	1299 - 1847 (+)	beta-hydroxylase	Scaffold biosynthesis	Sequence-based prediction	copy AA seq copy Nt seq
ACQ63619.1 cpz11	1867 - 2499 (+)	SAM-dependent methyltransferase	Tailoring (Methylation)	Sequence-based prediction	copy AA seq copy Nt seq
ACQ63620.1 cpz12	2496 - 3065 (+)	TmrB-like kinase	Unknown	Sequence-based prediction	copy AA seq copy Nt seq
ACQ63621.1 cpz13	3136 - 4461 (+)	amino-cyclopropyl transferase	Scaffold biosynthesis	Sequence-based prediction	copy AA seq copy Nt seq
ACQ63622.1 cpz14	4466 - 5740 (+)	transaldolase	Scaffold biosynthesis	Sequence-based prediction	copy AA seq copy Nt seq
ACQ63623.1 cpz15	5822 - 6646 (+)	Fe(II)-dependent UMP dioxygenase	Scaffold biosynthesis	Sequence-based prediction	copy AA seq copy Nt seq
ACQ63624.1 cpz16	6643 - 7344 (+)	nucleotidyltransferase	Precursor biosynthesis	Sequence-based prediction	copy AA seq copy Nt seq
ACQ63625.1 cpz17	7344 - 8477 (+)	glycosyltransferase	Scaffold biosynthesis	Sequence-based prediction	copy AA seq copy Nt seq
ACQ63626.1 cpz18	8474 - 9748 (+)	aminotransferase	Scaffold biosynthesis	Sequence-based prediction	copy AA seq copy Nt seq
ACQ63627.1 cpz19	9745 - 11127 (+)	pyrimidine-nucleoside phosphorylase	Scaffold biosynthesis	Sequence-based prediction	copy AA seq copy Nt seq
ACQ63628.1 cpz20	11120 - 12184 (+)	acyl-CoA synthase	Unknown	Sequence-based prediction	copy AA seq copy Nt seq
ACQ63629.1 cpz21	12184 - 13686 (+)	acyltransferase	Scaffold biosynthesis	Activity assay	copy AA seq copy Nt seq
ACQ63630.1 cpz22	13683 - 17399 (+)	ABC multidrug resistance transporter	Transport	Sequence-based prediction	copy AA seq copy Nt seq
ACQ63631.1 cpz23	17396 - 18430 (+)	acyltransferase	Scaffold biosynthesis	Sequence-based prediction	copy AA seq copy Nt seq
ACQ63632.1 cpz24	18716 - 20512 (+)	hypothetical protein	Unknown	Sequence-based prediction	copy AA seq copy Nt seq
ACQ63633.1 cpz25	20604 - 21629 (+)	acyl-CoA dehydrogenase	Unknown	Sequence-based prediction	copy AA seq copy Nt seq
ACQ63634.1 cpz26	21682 - 22935 (+)	SAM-dependent methyltransferase	Tailoring (Methylation)	Sequence-based prediction	copy AA seq copy Nt seq
ACQ63635.1 cpz27	22932 - 23510 (+)	TmrB-like kinase	Unknown	Sequence-based prediction	copy AA seq copy Nt seq
ACQ63636.1 cpz28	23591 - 24781 (+)	sugar O-methyltransferase	Tailoring (Methylation)	Sequence-based prediction	copy AA seq copy Nt seq
ACQ63637.1 cpz29	24825 - 25622 (+)	sugar O-methyltransferase	Tailoring (Methylation)	Sequence-based prediction	copy AA seq copy Nt seq
ACQ63638.1 cpz30	25619 - 26878 (+)	sugar O-methyltransferase	Tailoring (Methylation)	Sequence-based prediction	copy AA seq copy Nt seq
ACQ63639.1 cpz31	26882 - 28072 (+)	rhamnosyltransferase	Tailoring (Glycosylation)	Activity assay	copy AA seq copy Nt seq

Saccharide-specific information

Subclass	hybrid/tailoring
Glycosyltransferases	cpz31 Specificity: L-rhamnose Evidence: Sequence-based prediction
Glycosyltransferases	cpz17 Evidence: Sequence-based prediction

Annotation changelog

MIBiG version	Submitter	Notes
2.0	Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).	Migrated from v1.4
3.0	Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).	Remove duplicated citations
3.1	Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).	Update chemical activity to schema version 2.11

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