LOCUS       BGC0000898             12441 bp    DNA     linear   BCT 27-OCT-1998
DEFINITION  Streptomyces venezuelae desosamine biosynthetic gene cluster,
            complete sequence.
ACCESSION   BGC0000898
VERSION     BGC0000898
KEYWORDS    .
SOURCE      Streptomyces venezuelae
  ORGANISM  Streptomyces venezuelae
            Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
            Streptomyces.
COMMENT     ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2022-11-07 20:59:33
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 12441
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..12441
                     /db_xref="ATCC:15439"
                     /db_xref="taxon:54571"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces venezuelae"
                     /strain="ATCC 15439"
     subregion       1..12441
                     /aStool="mibig"
                     /contig_edge="True"
                     /label="Saccharide"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..12441
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            119..1327
                     /gene="desVIII"
     CDS             119..1327
                     /codon_start=1
                     /gene="desVIII"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007:cytochrome P450 (Score: 143.8; E-value: 1.1e-43)"
                     /gene_kind="biosynthetic-additional"
                     /product="putative tautomerase"
                     /protein_id="AAC68676.1"
                     /sec_met_domain="p450 (E-value: 9.3e-17, bitscore: 50.0,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTDDLTGALTQPPLGRTVRAVADRELGTHLLETRGIHWIHAANGD
                     PYATVLRGQADDPYPAYERVRARGALSFSPTGSWVTADHALAASILCSTDFGVSGADGV
                     PVPQQVLSYGEGCPLEREQVLPAAGDVPEGGQRAVVEGIHRETLEGLAPDPSASYAFEL
                     LGGFVRPAVTAAAAAVLGVPADRRADFADLLERLRPLSDSLLAPQSLRTVRAADGALAE
                     LTALLADSDDSPGALLSALGVTAAVQLTGNAVLALLAHPEQWRELCDRPGLAAAAVEET
                     LRYDPPVQLDARVVRGETELAGRRLPAGAHVVVLTAATGRDPEVFTDPERFDLARPDAA
                     AHLALHPAGPYGPVASLVRLQAEVALRTLAGRFPGLRQAGDVLRPRRAPVGRGPLSVPV
                     SSS"
     gene            1475..2755
                     /gene="desVII"
     CDS             1475..2755
                     /codon_start=1
                     /gene="desVII"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) MGT"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1062:glycosyltransferase, MGT family (Score: 378.3;
                     E-value: 8.6e-115)"
                     /gene_kind="biosynthetic-additional"
                     /product="glycosyl transferase"
                     /protein_id="AAC68677.1"
                     /sec_met_domain="MGT (E-value: 1.8e-149, bitscore: 487.4,
                     seeds: 193, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRVLLTSFAHHTHYYGLVPLAWALLAAGHEVRVASQPALTDTITG
                     SGLAAVPVGTDHLIHEYRVRMAGEPRPNHPAIAFDEARPEPLDWDHALGIEAILAPYFH
                     LLANNDSMVDDLVDFARSWQPDLVLWEPTTYAGAVAAQVTGAAHARVLWGPDVMGSARR
                     KFVALRDRQPPEHREDPTAEWLTWTLDRYGASFEEELLTGQFTIDPTPPSLRLDTGLPT
                     VGMRYVPYNGTSVVPDWLSEPPARPRVCLTLGVSAREVLGGDGVSQGDILEALADLDIE
                     LVATLDASQRAEIRNYPKHTRFTDFVPMHALLPSCSAIIHHGGAGTYATAVINAVPQVM
                     LAELWDAPVKARAVAEQGAGFFLPPAELTPQAVRDAVVRILDDPSVATAAHRLREETFG
                     DPTPAGIVPELERLAAQHRRPPADARH"
     gene            2845..3558
                     /gene="desVI"
     CDS             2845..3558
                     /codon_start=1
                     /gene="desVI"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1248:methyltransferase (Score: 233.8; E-value:
                     3.6e-71)"
                     /gene_kind="biosynthetic-additional"
                     /product="N,N-dimethyltransferase"
                     /protein_id="AAC68678.1"
                     /transl_table=11
                     /translation="MYEVDHADVYDLFYLGRGKDYAAEASDIADLVRSRTPEASSLLDV
                     ACGTGTHLEHFTKEFGDTAGLELSEDMLTHARKRLPDATLHQGDMRDFRLGRKFSAVVS
                     MFSSVGYLKTTEELGAAVASFAEHLEPGGVVVVEPWWFPETFADGWVSADVVRRDGRTV
                     ARVSHSVREGNATRMEVHFTVADPGKGVRHFSDVHLITLFHQAEYEAAFTAAGLRVEYL
                     EGGPSGRGLFVGVPA"
     gene            3625..6054
                     /gene="desR"
     CDS             3625..6054
                     /codon_start=1
                     /gene="desR"
                     /note="putative extracellular protein involved in a
                     resistance mechanism"
                     /product="beta-glucosidase"
                     /protein_id="AAC68679.1"
                     /transl_table=11
                     /translation="MTGKTRIPRVRRGRTTPRAFTLAVVGTLLAGTTVAAAAPGAADTA
                     NVQYTSRAAELVAQMTLDEKISFVHWALDPDRQNVGYLPGVPRLGIPELRAADGPNGIR
                     LVGQTATALPAPVALASTFDDTMADSYGKVMGRDGRALNQDMVLGPMMNNIRVPHGGRN
                     YETFSEDPLVSSRTAVAQIKGIQGAGLMTTAKHFAANNQENNRFSVNANVDEQTLREIE
                     FPAFEASSKAGAASFMCAYNGLNGKPSCGNDELLNNVLRTQWGFQGWVMSDWLATPGTD
                     AITKGLDQEMGVELPGDVPKGEPSPPAKFFGEALKTAVLNGTVPEAAVTRSAERIVGQM
                     EKFGLLLATPAPRPERDKAGAQAVSRKVAENGAVLLRNEGQALPLAGDAGKSIAVIGPT
                     AVDPKVTGLGSAHVVPDSAAAPLDTIKARAGAGATVTYETGEETFGTQIPAGNLSPAFN
                     QGHQLEPGKAGALYDGTLTVPADGEYRIAVRATGGYATVQLGSHTIEAGQVYGKVSSPL
                     LKLTKGTHKLTISGFAMSATPLSLELGWVTPAAADATIAKAVESARKARTAVVFAYDDG
                     TEGVDRPNLSLPGTQDKLISAVADANPNTIVVLNTGSSVLMPWLSKTRAVLDMWYPGQA
                     GAEATAALLYGDVNPSGKLTQSFPAAENQHAVAGDPTSYPGVDNQQTYREGIHVGYRWF
                     DKENVKPLFPFGHGLSYTSFTQSAPTVVRTSTGGLKVTVTVRNSGKRAGQEVVQAYLGA
                     SPNVTAPQAKKKLVGYTKVSLAAGEAKTVTVNVDRRQLQTGSSSADLRGSATVNVW"
     gene            complement(6143..7282)
                     /gene="desV"
     CDS             complement(6143..7282)
                     /NRPS_PKS="Domain: Aminotran_1_2 (47-220). E-value:
                     1.6e-07. Score: 22.6. Matches aSDomain:
                     nrpspksdomains_desV_Aminotran_1_2.1"
                     /codon_start=1
                     /gene="desV"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DegT_DnrJ_EryC1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1056:DegT/DnrJ/EryC1/StrS aminotransferase (Score:
                     398.3; E-value: 6e-121)"
                     /gene_kind="biosynthetic-additional"
                     /product="transaminase"
                     /protein_id="AAC68680.1"
                     /sec_met_domain="DegT_DnrJ_EryC1 (E-value: 1.5e-128,
                     bitscore: 418.2, seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSSRAETPRVPFLDLKAAYEELRAETDAAIARVLDSGRYLLGPEL
                     EGFEAEFAAYCETDHAVGVNSGMDALQLALRGLGIGPGDEVIVPSHTYIASWLAVSATG
                     ATPVPVEPHEDHPTLDPLLVEKAITPRTRALLPVHLYGHPADMDALRELADRHGLHIVE
                     DAAQAHGARYRGRRIGAGSSVAAFSFYPGKNLGCFGDGGAVVTGDPELAERLRMLRNYG
                     SRQKYSHETKGTNSRLDEMQAAVLRIRLAHLDSWNGRRSALAAEYLSGLAGLPGIGLPV
                     TAPDTDPVWHLFTVRTERRDELRSHLDARGIDTLTHYPVPVHLSPAYAGEAPPEGSLPR
                     AESFARQVLSLPIGPHLERPQALRVIDAVREWAERVDQA"
     aSDomain        complement(6623..7141)
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_desV_Aminotran_1_2.1"
                     /evalue="1.60E-07"
                     /label="desV_Aminotran_1_2.1"
                     /locus_tag="desV"
                     /protein_end="220"
                     /protein_start="47"
                     /score="22.6"
                     /tool="antismash"
                     /translation="FEAEFAAYCETDHAVGVNSGMDALQLALRGLGIGPGDEVIVPSHT
                     YIASWLAVSATGATPVPVEPHEDHPTLDPLLVEKAITPRTRALLPVHLYGHPADMDALR
                     ELADRHGLHIVEDAAQAHGARYRGRRIGAGSSVAAFSFYPGKNLGCFGDGGAVVTGDPE
                     LAERLRMLRN"
     gene            complement(7279..8292)
                     /gene="desIV"
     CDS             complement(7279..8292)
                     /NRPS_PKS="Domain: NAD_binding_4 (4-180). E-value: 4.4e-19.
                     Score: 60.4. Matches aSDomain:
                     nrpspksdomains_desIV_NAD_binding_4.1"
                     /codon_start=1
                     /gene="desIV"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) NAD_binding_4"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010:NAD-dependent epimerase/dehydratase (Score:
                     330.7; E-value: 1.5e-100)"
                     /gene_kind="biosynthetic-additional"
                     /product="TDP-glucose-4,6-dehydratase"
                     /protein_id="AAC68681.1"
                     /sec_met_domain="NAD_binding_4 (E-value: 7.2e-20, bitscore:
                     60.4, seeds: 102, tool: rule-based-clusters)"
                     /sec_met_domain="Polysacc_synt_2 (E-value: 1.4e-18,
                     bitscore: 56.2, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="RmlD_sub_bind (E-value: 2.1e-19, bitscore:
                     58.9, seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRLLVTGGAGFIGSHFVRQLLAGAYPDVPADEVIVLDSLTYAGNR
                     ANLAPVDADPRLRFVHGDIRDAGLLARELRGVDAIVHFAAESHVDRSIAGASVFTETNV
                     QGTQTLLQCAVDAGVGRVVHVSTDEVYGSIDSGSWTESSPLEPNSPYAASKAGSDLVAR
                     AYHRTYGLDVRITRCCNNYGPYQHPEKLIPLFVTNLLDGGTLPLYGDGANVREWVHTDD
                     HCRGIALVLAGGRAGEIYHIGGGLELTNRELTGILLDSLGADWSSVRKVADRKGHDLRY
                     SLDGGEIERELGYRPQVSFADGLARTVRWYRENRGWWEPLKATAPQLPATAVEVSA"
     aSDomain        complement(7753..8280)
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_desIV_NAD_binding_4.1"
                     /evalue="4.40E-19"
                     /label="desIV_NAD_binding_4.1"
                     /locus_tag="desIV"
                     /protein_end="180"
                     /protein_start="4"
                     /score="60.4"
                     /tool="antismash"
                     /translation="VTGGAGFIGSHFVRQLLAGAYPDVPADEVIVLDSLTYAGNRANLA
                     PVDADPRLRFVHGDIRDAGLLARELRGVDAIVHFAAESHVDRSIAGASVFTETNVQGTQ
                     TLLQCAVDAGVGRVVHVSTDEVYGSIDSGSWTESSPLEPNSPYAASKAGSDLVARAYHR
                     TYGLDVRITRCCN"
     CDS_motif       complement(8221..8283)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_desIV_0001"
                     /evalue="4.30E-03"
                     /label="PKSI-KR_m1"
                     /locus_tag="desIV"
                     /protein_end="24"
                     /protein_start="3"
                     /score="9.8"
                     /tool="antismash"
                     /translation="LVTGGAGFIGSHFVRQLLAGA"
     gene            complement(8424..9302)
                     /gene="desIII"
     CDS             complement(8424..9302)
                     /codon_start=1
                     /gene="desIII"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1064:glucose-1-phosphate
                     adenylyl/thymidylyltransferase (Score: 283.3; E-value:
                     3.5e-86)"
                     /gene_kind="biosynthetic-additional"
                     /product="alpha-D-glucose-1-phosphate
                     thymidylyltransferase"
                     /protein_id="AAC68682.1"
                     /transl_table=11
                     /translation="MKGIVLAGGSGTRLHPATSVISKQILPVYNKPMIYYPLSVLMLGG
                     IREIQIISTPQHIELFQSLLGNGRHLGIELDYAVQKEPAGIADALLVGAEHIGDDTCAL
                     ILGDNIFHGPGLYTLLRDSIARLDGCVLFGYPVKDPERYGVAEVDATGRLTDLVEKPVK
                     PRSNLAVTGLYLYDNDVVDIAKNIRPSPRGELEITDVNRVYLERGRAELVNLGRGFAWL
                     DTGTHDSLLRAAQYVQVLEERQGVWIAGLEEIAFRMGFIDAEACHGLGEGLSRTEYGSY
                     LMEIAGREGAP"
     gene            complement(9495..10952)
                     /gene="desII"
     CDS             complement(9495..10952)
                     /codon_start=1
                     /gene="desII"
                     /product="putative reductase"
                     /protein_id="AAC68683.1"
                     /transl_table=11
                     /translation="MTAPALSATAPAERCAHPGADLGAAVHAVGQTLAAGGLVPPDEAG
                     TTARHLVRLAVRYGNSPFTPLEEARHDLGVDRDAFRRLLALFGQVPELRTAVETGPAGA
                     YWKNTLLPLEQRGVFDAALARKPVFPYSVGLYPGPTCMFRCHFCVRVTGARYDPSALDA
                     GNAMFRSVIDEIPAGNPSAMYFSGGLEPLTNPGLGSLAAHATDHGLRPTVYTNSFALTE
                     RTLERQPGLWGLHAIRTSLYGLNDEEYEQTTGKKAAFRRVRENLRRFQQLRAERESPIN
                     LGFAYIVLPGRASRLLDLVDFIADLNDAGQGRTIDFVNIREDYSGRDDGKLPQEERAEL
                     QEALNAFEERVRERTPGLHIDYGYALNSLRTGADAELLRIKPATMRPTAHPQVAVQVDL
                     LGDVYLYREAGFPDLDGATRYIAGRVTPDTSLTEVVRDFVERGGEVAAVDGDEYFMDGF
                     DQVVTARLNQLERDAADGWEEARGFLR"
     gene            complement(10949..12196)
                     /gene="desI"
     CDS             complement(10949..12196)
                     /NRPS_PKS="Domain: Aminotran_1_2 (54-181). E-value: 4e-09.
                     Score: 27.8. Matches aSDomain:
                     nrpspksdomains_desI_Aminotran_1_2.1"
                     /codon_start=1
                     /gene="desI"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DegT_DnrJ_EryC1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1056:DegT/DnrJ/EryC1/StrS aminotransferase (Score:
                     361; E-value: 1.2e-109)"
                     /gene_kind="biosynthetic-additional"
                     /product="4-dehydrase"
                     /protein_id="AAC68684.1"
                     /sec_met_domain="DegT_DnrJ_EryC1 (E-value: 2.4e-107,
                     bitscore: 348.5, seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKSALSDLAFFGGPAAFDQPLLVGRPNRIDRARLYERLDRALDSQ
                     WLSNGGPLVREFEERVAGLAGVRHAVATCNATAGLQLLAHAAGLTGEVIMPSMTFAATP
                     HALRWIGLTPVFADIDPDTGNLDPDQVAAAVTPRTSAVVGVHLWGRPCAADQLRKVADE
                     HGLRLYFDAAHALGCAVDGRPAGSLGDAEVFSFHATKAVNAFEGGAVVTDDADLAARIR
                     ALHNFGFDLPGGSPAGGTNAKMSEAAAAMGLTSLDAFPEVIDRNRRNHAAYREHLADLP
                     GVLVADHDRHGLNNHQYVIVEIDEATTGIHRDLVMEVLKAEGVHTRAYFSPGCHELEPY
                     RGQPHAPLPHTERLAARVLSLPTGTAIGDDDIRRVADLLRLCATRGRELTARHRDTAPA
                     PLAAPQTSTPTIGRSR"
     aSDomain        complement(11654..12034)
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_desI_Aminotran_1_2.1"
                     /evalue="4.00E-09"
                     /label="desI_Aminotran_1_2.1"
                     /locus_tag="desI"
                     /protein_end="181"
                     /protein_start="54"
                     /score="27.8"
                     /tool="antismash"
                     /translation="REFEERVAGLAGVRHAVATCNATAGLQLLAHAAGLTGEVIMPSMT
                     FAATPHALRWIGLTPVFADIDPDTGNLDPDQVAAAVTPRTSAVVGVHLWGRPCAADQLR
                     KVADEHGLRLYFDAAHALGCAVD"
ORIGIN
        1 agatctgcaa cgacatctcc gaccacctgc tcgtcacccg cggcgcgccc gatgcccgcg
       61 tcgtgcagcc cccgaccagc cttatcgaag gagcggcgaa gagatggcag aacccacggt
      121 gaccgacgac ctgacggggg ccctcacgca gcccccgctg ggccgcaccg tccgcgcggt
      181 ggccgaccgt gaactcggca cccacctcct ggagacccgc ggcatccact ggatccacgc
      241 cgcgaacggc gacccgtacg ccaccgtgct gcgcggccag gcggacgacc cgtatcccgc
      301 gtacgagcgg gtgcgtgccc gcggcgcgct ctccttcagc ccgacgggca gctgggtcac
      361 cgccgatcac gccctggcgg cgagcatcct ctgctcgacg gacttcgggg tctccggcgc
      421 cgacggcgtc ccggtgccgc agcaggtcct ctcgtacggg gagggctgtc cgctggagcg
      481 cgagcaggtg ctgccggcgg ccggtgacgt gccggagggc gggcagcgtg ccgtggtcga
      541 ggggatccac cgggagacgc tggagggtct cgcgccggac ccgtcggcgt cgtacgcctt
      601 cgagctgctg ggcggtttcg tccgcccggc ggtgacggcc gctgccgccg ccgtgctggg
      661 tgttcccgcg gaccggcgcg cggacttcgc ggatctgctg gagcggctcc ggccgctgtc
      721 cgacagcctg ctggccccgc agtccctgcg gacggtacgg gcggcggacg gcgcgctggc
      781 cgagctcacg gcgctgctcg ccgattcgga cgactccccc ggggccctgc tgtcggcgct
      841 cggggtcacc gcagccgtcc agctcaccgg gaacgcggtg ctcgcgctcc tcgcgcatcc
      901 cgagcagtgg cgggagctgt gcgaccggcc cgggctcgcg gcggccgcgg tggaggagac
      961 cctccgctac gacccgccgg tgcagctcga cgcccgggtg gtccgcgggg agacggagct
     1021 ggcgggccgg cggctgccgg ccggggcgca tgtcgtcgtc ctgaccgccg cgaccggccg
     1081 ggacccggag gtcttcacgg acccggagcg cttcgacctc gcgcgccccg acgccgccgc
     1141 gcacctcgcg ctgcaccccg ccggtccgta cggcccggtg gcgtccctgg tccggcttca
     1201 ggcggaggtc gcgctgcgga ccctggccgg gcgtttcccc gggctgcggc aggcggggga
     1261 cgtgctccgc ccccgccgcg cgcctgtcgg ccgcgggccg ctgagcgtcc cggtcagcag
     1321 ctcctgagac accggggccc cggtccgccc ggcccccctt cggacggacc ggacggctcg
     1381 gaccacgggg acggctcaga ccgtcccgtg tgtccccgtc cggctcccgt ccgccccatc
     1441 ccgcccctcc accggcaagg aaggacacga cgccatgcgc gtcctgctga cctcgttcgc
     1501 acatcacacg cactactacg gcctggtgcc cctggcctgg gcgctgctcg ccgccgggca
     1561 cgaggtgcgg gtcgccagcc agcccgcgct cacggacacc atcaccgggt ccgggctcgc
     1621 cgcggtgccg gtcggcaccg accacctcat ccacgagtac cgggtgcgga tggcgggcga
     1681 gccgcgcccg aaccatccgg cgatcgcctt cgacgaggcc cgtcccgagc cgctggactg
     1741 ggaccacgcc ctcggcatcg aggcgatcct cgccccgtac ttccatctgc tcgccaacaa
     1801 cgactcgatg gtcgacgacc tcgtcgactt cgcccggtcc tggcagccgg acctggtgct
     1861 gtgggagccg acgacctacg cgggcgccgt cgccgcccag gtcaccggtg ccgcgcacgc
     1921 ccgggtcctg tgggggcccg acgtgatggg cagcgcccgc cgcaagttcg tcgcgctgcg
     1981 ggaccggcag ccgcccgagc accgcgagga ccccaccgcg gagtggctga cgtggacgct
     2041 cgaccggtac ggcgcctcct tcgaagagga gctgctcacc ggccagttca cgatcgaccc
     2101 gaccccgccg agcctgcgcc tcgacacggg cctgccgacc gtcgggatgc gttatgttcc
     2161 gtacaacggc acgtcggtcg tgccggactg gctgagtgag ccgcccgcgc ggccccgggt
     2221 ctgcctgacc ctcggcgtct ccgcgcgtga ggtcctcggc ggcgacggcg tctcgcaggg
     2281 cgacatcctg gaggcgctcg ccgacctcga catcgagctc gtcgccacgc tcgacgcgag
     2341 tcagcgcgcc gagatccgca actacccgaa gcacacccgg ttcacggact tcgtgccgat
     2401 gcacgcgctc ctgccgagct gctcggcgat catccaccac ggcggggcgg gcacctacgc
     2461 gaccgccgtg atcaacgcgg tgccgcaggt catgctcgcc gagctgtggg acgcgccggt
     2521 caaggcgcgg gccgtcgccg agcagggggc ggggttcttc ctgccgccgg ccgagctcac
     2581 gccgcaggcc gtgcgggacg ccgtcgtccg catcctcgac gacccctcgg tcgccaccgc
     2641 cgcgcaccgg ctgcgcgagg agaccttcgg cgaccccacc ccggccggga tcgtccccga
     2701 gctggagcgg ctcgccgcgc agcaccgccg cccgccggcc gacgcccggc actgagccgc
     2761 acccctcgcc ccaggcctca cccctgtatc tgcgccgggg gacgcccccg gcccaccctc
     2821 cgaaagaccg aaagcaggag caccgtgtac gaagtcgacc acgccgacgt ctacgacctc
     2881 ttctacctgg gtcgcggcaa ggactacgcc gccgaggcct ccgacatcgc cgacctggtg
     2941 cgctcccgta cccccgaggc ctcctcgctc ctggacgtgg cctgcggtac gggcacgcat
     3001 ctggagcact tcaccaagga gttcggcgac accgccggcc tggagctgtc cgaggacatg
     3061 ctcacccacg cccgcaagcg gctgcccgac gccacgctcc accagggcga catgcgggac
     3121 ttccggctcg gccggaagtt ctccgccgtg gtcagcatgt tcagctccgt cggctacctg
     3181 aagacgaccg aggaactcgg cgcggccgtc gcctcgttcg cggagcacct ggagcccggt
     3241 ggcgtcgtcg tcgtcgagcc gtggtggttc ccggagacct tcgccgacgg ctgggtcagc
     3301 gccgacgtcg tccgccgtga cgggcgcacc gtggcccgtg tctcgcactc ggtgcgggag
     3361 gggaacgcga cgcgcatgga ggtccacttc accgtggccg acccgggcaa gggcgtgcgg
     3421 cacttctccg acgtccatct catcaccctg ttccaccagg ccgagtacga ggccgcgttc
     3481 acggccgccg ggctgcgcgt cgagtacctg gagggcggcc cgtcgggccg tggcctcttc
     3541 gtcggcgtcc ccgcctgagc accgcccaag accccccggg gcgggacgtc ccgggtgcac
     3601 caagcaaaga gagagaaacg aaccgtgaca ggtaagaccc gaataccgcg tgtccgccgc
     3661 ggccgcacca cgcccagggc cttcaccctg gccgtcgtcg gcaccctgct ggcgggcacc
     3721 accgtggcgg ccgccgctcc cggcgccgcc gacacggcca atgttcagta cacgagccgg
     3781 gcggcggagc tcgtcgccca gatgacgctc gacgagaaga tcagcttcgt ccactgggcg
     3841 ctggaccccg accggcagaa cgtcggctac cttcccggcg tgccgcgtct gggcatcccg
     3901 gagctgcgtg ccgccgacgg cccgaacggc atccgcctgg tggggcagac cgccaccgcg
     3961 ctgcccgcgc cggtcgccct ggccagcacc ttcgacgaca ccatggccga cagctacggc
     4021 aaggtcatgg gccgcgacgg tcgcgcgctc aaccaggaca tggtcctggg cccgatgatg
     4081 aacaacatcc gggtgccgca cggcggccgg aactacgaga ccttcagcga ggaccccctg
     4141 gtctcctcgc gcaccgcggt cgcccagatc aagggcatcc agggtgcggg tctgatgacc
     4201 acggccaagc acttcgcggc caacaaccag gagaacaacc gcttctccgt gaacgccaat
     4261 gtcgacgagc agacgctccg cgagatcgag ttcccggcgt tcgaggcgtc ctccaaggcc
     4321 ggcgcggcct ccttcatgtg tgcctacaac ggcctcaacg ggaagccgtc ctgcggcaac
     4381 gacgagctcc tcaacaacgt gctgcgcacg cagtggggct tccagggctg ggtgatgtcc
     4441 gactggctcg ccaccccggg caccgacgcc atcaccaagg gcctcgacca ggagatgggc
     4501 gtcgagctcc ccggcgacgt cccgaagggc gagccctcgc cgccggccaa gttcttcggc
     4561 gaggcgctga agacggccgt cctgaacggc acggtccccg aggcggccgt gacgcggtcg
     4621 gcggagcgga tcgtcggcca gatggagaag ttcggtctgc tcctcgccac tccggcgccg
     4681 cggcccgagc gcgacaaggc gggtgcccag gcggtgtccc gcaaggtcgc cgagaacggc
     4741 gcggtgctcc tgcgcaacga gggccaggcc ctgccgctcg ccggtgacgc cggcaagagc
     4801 atcgcggtca tcggcccgac ggccgtcgac cccaaggtca ccggcctggg cagcgcccac
     4861 gtcgtcccgg actcggcggc ggcgccactc gacaccatca aggcccgcgc gggtgcgggt
     4921 gcgacggtga cgtacgagac gggtgaggag accttcggga cgcagatccc ggcggggaac
     4981 ctcagcccgg cgttcaacca gggccaccag ctcgagccgg gcaaggcggg ggcgctgtac
     5041 gacggcacgc tgaccgtgcc cgccgacggc gagtaccgca tcgcggtccg tgccaccggt
     5101 ggttacgcca cggtgcagct cggcagccac accatcgagg ccggtcaggt ctacggcaag
     5161 gtgagcagcc cgctcctcaa gctgaccaag ggcacgcaca agctcacgat ctcgggcttc
     5221 gcgatgagtg ccaccccgct ctccctggag ctgggctggg tgacgccggc ggcggccgac
     5281 gcgacgatcg cgaaggccgt ggagtcggcg cggaaggccc gtacggcggt cgtcttcgcc
     5341 tacgacgacg gcaccgaggg cgtcgaccgt ccgaacctgt cgctgccggg tacgcaggac
     5401 aagctgatct cggctgtcgc ggacgccaac ccgaacacga tcgtggtcct caacaccggt
     5461 tcgtcggtgc tgatgccgtg gctgtccaag acccgcgcgg tcctggacat gtggtacccg
     5521 ggccaggcgg gcgccgaggc caccgccgcg ctgctctacg gtgacgtcaa cccgagcggc
     5581 aagctcacgc agagcttccc ggccgccgag aaccagcacg cggtcgccgg cgacccgaca
     5641 agctacccgg gcgtcgacaa ccagcagacg taccgcgagg gcatccacgt cgggtaccgc
     5701 tggttcgaca aggagaacgt caagccgctg ttcccgttcg ggcacggcct gtcgtacacc
     5761 tcgttcacgc agagcgcccc gaccgtcgtg cgtacgtcca cgggtggtct gaaggtcacg
     5821 gtcacggtcc gcaacagcgg gaagcgcgcc ggccaggagg tcgtccaggc gtacctcggt
     5881 gccagcccga acgtgacggc tccgcaggcg aagaagaagc tcgtgggcta cacgaaggtc
     5941 tcgctcgccg cgggcgaggc gaagacggtg acggtgaacg tcgaccgccg tcagctgcag
     6001 accggttcgt cctccgccga cctgcggggc agcgccacgg tcaacgtctg gtgacgtgac
     6061 gccgtgaaag cggcggtgcc cgccacccgg gagggtggcg ggcaccgctt tttcggcctg
     6121 ctgggtctac cggaccacct gactaggcct ggtcgacccg ctcggcccat tcgcgcacgg
     6181 cgtcgatcac ccgcagcgcc tgcgggcgct ccaggtgcgg gccgatcggc aggctgagga
     6241 cctgccgcgc gaagctctcg gcccgcggga gcgagccttc cggcggtgcc tcgcccgcgt
     6301 aggcgggcga gaggtgcacg ggtaccgggt agtgcgtgag ggtgtcgatg ccgcgggcgt
     6361 cgaggtggct gcgcagctcg tcgcggcgct cggtgcgcac ggtgaagagg tgccagaccg
     6421 ggtcggtgtc gggcgcggtc accggcaggc cgatgccggg cagtccggcg agcccggaga
     6481 ggtactccgc ggccagcgcc gacctgcggc cgttccagct gtccaggtgg gcgagccgga
     6541 tccgcagcac ggcggcctgc atctcgtcca ggcgggagtt ggtgcccttc gtctcgtggc
     6601 tgtacttctg ccgcgagccg tagttgcgga gcatccggag ccgttcggcg agctcggggt
     6661 cgccggtgac gacggcgccg ccgtcgccga agcagccgag gttcttgccc gggtagaagc
     6721 tgaacgcggc caccgacgac ccggcgccga tccgccggcc ccggtagcgg gcgccgtggg
     6781 cctgcgcggc gtcctcgacg atgtgcaggc cgtgccggtc cgcgagctcg cggagggcgt
     6841 ccatgtcggc ggggtgcccg tagaggtgga cggggaggag cgcccgggtg cggggggtga
     6901 tcgccttctc gacgagcagc gggtccaggg tggggtggtc ctcgtgcggc tcgacgggca
     6961 cgggggtcgc gccggtggcg gacaccgcga gccagctggc gatgtacgtg tgcgagggga
     7021 cgatcacctc gtccccgggt ccgatgccga ggccgcggag ggcgagctgg agggcgtcca
     7081 tcccgctgtt cacgccgacg gcgtggtccg tctcgcagta cgcggcgaac tccgcctcga
     7141 atccttcgag ttcgggtccg aggaggtagc gccccgagtc gaggacgcgg gcgatcgcgg
     7201 cgtcggtctc cgcgcggagc tcctcgtagg cggccttgag gtcgaggaag gggacgcggg
     7261 gggtctcggc gcggctgctc acgcggacac ctccacggcg gtggcgggca gctgcggggc
     7321 ggtcgccttg agcggctccc accagccgcg gttctcccgg taccagcgga cggtccgcgc
     7381 gaggccgtcc gcgaaggaga cctgcgggcg gtagccgagc tcgcgctcga tctcgccgcc
     7441 gtcgagggag tagcgcaggt cgtggccctt gcggtcggcg accttccgga ccgaggacca
     7501 gtcggcgccg agcgagtcca ggaggatgcc ggtgagttcg cggttggtca gctccaggcc
     7561 gccgccgatg tggtagatct cgccggcccg gccgcccgcg aggacgagcg cgatgccccg
     7621 gcagtggtcg tcggtgtgca cccactcgcg gacgttcgcg ccgtcgccgt acagcgggag
     7681 cgtcccgccg tcgaggaggt tcgtcacgaa gagggggatg agcttctcgg ggtgctggta
     7741 cggcccgtag ttgttgcagc agcgggtgat ccgtacgtcg aggccgtacg tccggtggta
     7801 ggcgcgggca acgaggtcgg agccggcctt ggacgccgcg tagggcgagt tgggctccag
     7861 cgggctgctc tcggtccagg agccggagtc gatcgacccg tacacctcgt cggtggagac
     7921 gtgcacgacc cggccgacgc cggcgtcgac ggcgcactgg agcagcgtct gcgtgccctg
     7981 cacgttggtc tcggtgaaca cggacgcgcc cgcgatggag cggtccacgt ggctctcggc
     8041 cgcgaagtgg acgatggcgt ccacgccgcg cagttcccgg gcgaggaggc cggcgtcgcg
     8101 gatgtcgccg tggacgaagc gcagtcgcgg gtccgcgtcc accggggcga ggttggcgcg
     8161 gttgcccgcg taggtgaggc tgtccaggac gatcacctca tcggcgggca cgtcggggta
     8221 cgccccggcg aggagctgcc gcacgaagtg cgagccgatg aagcccgcac ctccggtcac
     8281 cagaagccgc actgccgtct tcctttcggt cgcgctgtag gtcgcggtgt gggtcgcact
     8341 gtcggtggcg gtgcgggtcg cggtgtgggt cgcactgtcg gtggcgctgt cggtcgtggg
     8401 aacgcgtcgg ccgcgaggtg ccctcacggg gctccctcgc ggccggcgat ctccatcaga
     8461 tagctgccgt actcggtgcg ggagaggcct tctcccaggc cgtgacaggc ctcggcgtcg
     8521 atgaagccca tgcggaaggc gatctcctca aggcccgcga tccagacgcc ctgccgctcc
     8581 tccaggacct ggacgtactg ggcggcccgc aggagcgagt cgtgggtgcc ggtgtccagc
     8641 caggcgaagc cgcggcccag gttgacgagt tcggcccggc cccgctccag gtagacgcgg
     8701 ttgacgtcgg tgatctccag ctcgccgcgc ggcgagggcc ggatgttctt ggcgatgtcg
     8761 acgacgtcgt tgtcgtagag gtagaggccg gtgacggcga ggttggagcg cggcttgacg
     8821 ggcttctcga cgaggtcggt cagccggccc gtcgcgtcca cctcggcgac gccgtaccgc
     8881 tcggggtcct tgaccgggta gccgaagagc acgcagccgt cgaggcgcgc gatgctgtcc
     8941 cgcaggagcg tgtagaggcc gggcccgtgg aagatgttgt cgcccaggat cagggcgcag
     9001 gtgtcgtcgc cgatgtgctc ggctccgacg agaagtgcgt ccgcgattcc tgcgggctct
     9061 ttctggaccg catagtcgag ttctattccc aggtgcctgc cgtttccgag aagcgactgg
     9121 aagagttcga tgtgctgggg ggtcgagatg atttgaatct cgcgaatacc gccgagcatg
     9181 agaaccgaca gcggatagta gatcatcggt ttgttgtaga ccggaagaat ctgcttcgaa
     9241 atgaccgagg tcgccggatg cagccgagtt ccgctcccgc cggccaggac tattcccttc
     9301 attctcggaa actagcagca gggcgccggt gataacggtc ggcgtggcga gttagggggg
     9361 cgctaggggc tgcgcagggg gagtgtcacc acccctttgg ggggtgggaa aacaccgagg
     9421 gcccggccgg acggccgggc cctcaggtgg ggggatcgtg ggggggggat cggggggatc
     9481 ggggcgggtg cgggtcagcg caggaagccg cgggcctcct cccagccgtc cgcggcgtcg
     9541 cgctccagct ggttcaggcg ggcggtgacg acctgatcga agccgtccat gaagtactcg
     9601 tcgccgtcga cggccgccac ctcgccgccg cgctcgacga agtccctgac gacctcggtg
     9661 agggaggtgt cgggggtcac gcggcccgcg atgtagcggg tcgcgccgtc caggtcgggg
     9721 aagccggcct cgcggtacag gtacacgtcg ccgaggagat cgacctgcac cgcgacctgc
     9781 gggtgcgcgg tgggccgcat ggtggcgggc ttgatccgca gcagttcggc gtcggccccg
     9841 gtgcgcaggc tgttcagggc gtagccgtag tcgatgtgga gtccgggggt gcgctcgcgg
     9901 acccgctcct cgaaggcgtt gagggcctcc tggagctcgg cccgctcctc ctgcggcagc
     9961 ttgccgtcgt cacggccgct gtagtcctcg cgaatgttga cgaagtcgat cgtcctgccc
    10021 tgcccggcgt cgttgaggtc ggcgatgaag tcgaccaggt cgagcaggcg ggaggcacgg
    10081 cccgggagca cgatgtaggc gaagccgagg ttgatcggcg actcgcgctc ggcgcgcagc
    10141 tgctggaagc ggcgcaggtt ctcgcggacg cggcggaagg cggccttctt gccggtggtc
    10201 tgctcgtact cctcgtcgtt gaggccgtag agcgaggtgc ggatggcgtg caggccccag
    10261 aggccgggct ggcgctccag ggtgcgctcg gtgagcgcga aggagttcgt gtagacggtg
    10321 ggccgcaggc cgtggtcggt ggcgtgcgcg gccaggctcc cgaggccggg gttggtgagc
    10381 ggctccaggc cgccggagaa gtacatcgcc gaggggttgc ccgcgggtat ctcgtcgatg
    10441 accgaccgga acatggcgtt gccggcgtcg agggcggacg ggtcgtagcg ggcgccggtc
    10501 acacggacgc agaagtggca gcggaacatg caggtcgggc cggggtagag gccgacgctg
    10561 tacgggaaga cgggcttcct ggcgagcgcc gcgtcgaaga cgccgcgctg ttcgagcggg
    10621 agcagggtgt tcttccagta cgccccggcg gggccggtct cgaccgcggt gcggagctcc
    10681 gggacctgcc cgaacagggc gaggaggcgc cggaaggcgt cccggtcgac gcccaggtcg
    10741 tggcgggcct cctccagcgg ggtgaagggg ctgttgccgt agcgcacggc gagccggacg
    10801 aggtggcggg cggtcgttcc ggcctcgtcg ggcggcacga ggccgccggc ggcgagggtc
    10861 tggccgacgg cgtggaccgc cgcccccaga tcggctccgg ggtgcgcgca gcgttcggcc
    10921 ggggcggtgg cggaaagggc gggggcggtc atcgggagcg tccaatcgtg ggcgtggatg
    10981 tctggggggc cgcgagcggg gcgggggccg tgtcgcggtg gcgcgcggtc agttcgcggc
    11041 cgcgggtcgc gcagagacgc agcaggtcgg cgacccggcg gatgtcgtcg tcgccgatgg
    11101 cggtgccggt cggcagggac agcacgcgcg cggcgaggcg ttcggtgtgc ggcagcgggg
    11161 cgtgcggctg cccgcggtac ggctccagct cgtggcagcc cggcgagaag taggcgcggg
    11221 tgtgcacgcc ttcggccttc aggacctcca tgacgaggtc gcggtggatg ccggtggtgg
    11281 cctcgtcgat ctcgacgatc acgtactggt ggttgttgag gccgtggcgg tcgtggtcgg
    11341 cgacgaggac gccggggagg tccgcgaggt gctcgcggta ggcggcgtgg ttgcgccggt
    11401 tccggtcgat gacctcggga aacgcgtcga gggaggtgag gcccatggcg gcggcggcct
    11461 cgctcatctt ggcgttggtc ccgccggcgg ggctgccgcc gggcaggtcg aagccgaagt
    11521 tgtggagggc gcggatccgg gcggcgaggt cggcgtcgtc ggtgacgacg gcgccgccct
    11581 cgaaggcgtt gacggccttg gtggcgtgga agctgaagac ctcggcgtcg ccgaggctgc
    11641 cggcgggccg gccgtcgacc gcgcagccga gggcgtgcgc ggcgtcgaag tacagccgca
    11701 ggccgtgctc gtcggcgacc ttccgcagct ggtcggcggc gcaggggcgg ccccagaggt
    11761 ggacgccgac gacggccgag gtgcggggtg tgaccgcggc ggccacctgg tccgggtcga
    11821 ggttgccggt gtccgggtcg atgtcggcga agaccggggt gaggccgatc cagcgcagtg
    11881 cgtgcggggt ggcggcgaac gtcatcgacg gcatgatcac ttcgccggtg aggccggcgg
    11941 cgtgcgcgag gagctggagc ccggccgtgg cgttgcaggt ggccacggca tgccggaccc
    12001 cggcgagccc ggcgacgcgc tcctcgaact cgcggacgag cgggccgccg ttggacagcc
    12061 actggctgtc gagggcccgg tcgagccgct cgtacagcct ggcgcggtcg atgcggttgg
    12121 gccgccccac gaggagcggc tggtcgaaag cggcggggcc gccgaagaat gcgaggtcgg
    12181 ataaggcgct tttcacggat gttccctccg ggccaccgtc acgaaatgat tcgccgatcc
    12241 gggaatcccg aacgaggtcg ccgcgctcca ccgtgacgta cgacgagatg gtcgattgtg
    12301 gtggtcgatt tcggggggac tctaatccgc gcggaacggg accgacaaga gcacgctatg
    12361 cgctctcgat gtgcttcgga tcacatccgc ctccggggta ttccatcggc ggcccgaatg
    12421 tgatgatcct tgacaggatc c
//
