LOCUS       BGC0000947             16122 bp    DNA     linear   BCT 20-SEP-2007
DEFINITION  Vibrio alginolyticus vibrioferrin biosynthesis and transportation
            gene cluster, complete sequence.
ACCESSION   BGC0000947
VERSION     BGC0000947
KEYWORDS    .
SOURCE      Vibrio alginolyticus
  ORGANISM  Vibrio alginolyticus
            Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales;
            Vibrionaceae; Vibrio.
COMMENT     On Jan 3, 2007 this sequence version replaced DQ201184.1.
            ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2022-11-07 21:08:51
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 16122
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..16122
                     /db_xref="taxon:663"
                     /mol_type="genomic DNA"
                     /organism="Vibrio alginolyticus"
                     /strain="MVPO1"
     subregion       1..16122
                     /aStool="mibig"
                     /contig_edge="True"
                     /label="Other"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..16122
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            complement(154..1356)
                     /gene="pvsE"
     CDS             complement(154..1356)
                     /codon_start=1
                     /gene="pvsE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Orn_DAP_Arg_deC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Orn_Arg_deC_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1264:diaminopimelate decarboxylase (Score: 324.1;
                     E-value: 1.4e-98)"
                     /gene_kind="biosynthetic-additional"
                     /note="vibrioferrin biosynthesis protein"
                     /product="PvsE"
                     /protein_id="ABM30201.1"
                     /sec_met_domain="Orn_DAP_Arg_deC (E-value: 2.3e-18,
                     bitscore: 55.9, seeds: 500, tool: rule-based-clusters)"
                     /sec_met_domain="Orn_Arg_deC_N (E-value: 8.5e-35, bitscore:
                     109.8, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSNSLSLPTLVCQQVHTLAQQQSQPLCAYVYDLSALEEHIKQLRH
                     VLPRNVELFYAAKANPSGPILKTLAPYVDGFEAASGGELTHLHQQQLDKPLIFGGPGKM
                     LSELQQAIELDVDAFHVESLTELQRIGALTERLNRPASIFLRMNIDIGDITLSKLAMGG
                     KPTPFGLDESELSNALMLLRDFPQVSLKGFHFHLMSHQLDVERHLALMQRYFQVVKTWQ
                     QEFKLSELMINLGGGMGINYQNPEQHFPWMEFCDKLEFLIAKEQVQDWTLRFECGRFIT
                     AACGYYVMEVLDIKQNLGENFVIARGGTHHFRTPAAQSHDHPFVILKSEQHHEISHPIQ
                     HAQATFVGQLCTPKDVLARNQHVAHVDIGDYVVFTLAGAYAWNISHQNFLMHEPPIFHY
                     F"
     misc_feature    154..15744
                     /note="vibrioferrin biosynthesis and transportation gene
                     cluster"
     gene            complement(1349..3178)
                     /gene="pvsD"
     CDS             complement(1349..3178)
                     /codon_start=1
                     /gene="pvsD"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     siderophore: IucA_IucC"
                     /gene_kind="biosynthetic"
                     /note="vibrioferrin biosynthesis protein"
                     /product="PvsD"
                     /protein_id="ABM30202.1"
                     /sec_met_domain="IucA_IucC (E-value: 3.4e-67, bitscore:
                     216.0, seeds: 168, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLQTQSSQTSELRAHEVQLNREKAQLNTIMGVLNCYLREFALPNQ
                     QVEWHYQSTSLPQTLKRNYPAKQRIAVHLSQQNGVLVLPIHYASKLGKIKLAELPWAKM
                     PGSGWAKLDATQTLTLLLNYLKQVLAIPFNHELIEQLENSVLVTEQFLNVAPKSHHNEF
                     IASEQSLIWGHTFHPTPKSRSGVTMDDLLAFSPEVGAQVPLYWFEVDTSLLDVLSSDGE
                     ANPAQRMLQRLAAQHATSNGKTLYPCHPWEGYTILSNPSVKRAIEQGKITPLGLGGEKM
                     QPTSSVRTLYHPDMDWFAKFSINVRLTNCVRKNAWYELDSAVQLTSILKPIRENEQLHN
                     PVFKVMTEPYATTLNLESVAEHNWDDNTKARESFGILYRENFSLSEVDILKPTLAGALF
                     AYDNNGNSRIAQRLKNKARTTQSRYSDIATLWFERYLHCLIPGVFNYYFKHGVAFEPHL
                     QNTLIGFEQEMPCCVWIRDLEGTKLLPEFWPAETLTDLSERARQSVYYSREHGWNRIGY
                     CTFINNISEAIFFIAEGDEPLEQTLWNAVQSAIVRWQSVNGKQPELEALLNGGHFPSKN
                     NFTTRLMQKADKESGYTQVATPWQQHEKGAQHV"
     gene            complement(3091..4356)
                     /gene="pvsC"
     CDS             complement(3091..4356)
                     /codon_start=1
                     /gene="pvsC"
                     /note="multi-drug efflux pump-like protein"
                     /product="PvsC"
                     /protein_id="ABM30203.1"
                     /transl_table=11
                     /translation="MTKLIYSVLLCHFIAAFAALGMPLFLPVVLPTLGASIDADWAGVL
                     FILPTFCTALAARFWGNFADRYGRRRSLLRAQLGLAAGFALCGFADNLAMFVFGLIVQG
                     TFGGTMAASNSYLSSQIKEAKTLGKSLNKTQLSARLALIVAPIGLGWSLSETNPLNAYL
                     WLCVLPLIAMGITLTLPVDKPADQNLEKPNSEPQQEKPVAQKNAMHAIRWIFFAQFGFA
                     FSMVVTFPYFSPFVQQYGITNHAWIGFLYALPHGVYLLLAGKIHVLSDGLNRHQPLRTL
                     WLGFALLAVSSALHLIASETALIAARIIFGIAMVCCYHGLHQHLASNLDRKLSGKVFSR
                     FDAMSKWGGVAAGFCASFISSLGLLEVPFLLSAIVSVITLIALVLHFKIWKSTCYKLNP
                     LRLASCARMRCSSTVRKPNSTR"
     gene            complement(4674..6194)
                     /gene="pvsB"
     CDS             complement(4674..6194)
                     /codon_start=1
                     /gene="pvsB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     siderophore: IucA_IucC"
                     /gene_kind="biosynthetic"
                     /product="PvsB"
                     /protein_id="ABM30204.1"
                     /sec_met_domain="IucA_IucC (E-value: 2.5e-46, bitscore:
                     147.7, seeds: 168, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNDNALYLTQRLIDTCLREDMFGILSKAKFSRDAPKGVIVPQDEQ
                     VWLIFANSDFTLYLPVAPAYYMQHWRYGHANDETPPGWWVEKNGQIEHQQHYKEWIALL
                     TTLAHESSHELLAGYLQELECAEKHKALCDQAFHHHADHISQPISKLGSWHQKLLLADQ
                     IASYLDHPYYPTARAKFGLSDEDLAKYAPEFAQSFALHWIAIEKSLLTLTSEQPDCWPT
                     MEQVGLPADFALSHVLFPAHPLTLRSLEALPEGMIEAPLTYLDVTPTLSVRTVVVNDAP
                     HIHIKVPLIMRSLGTKNIRLIKPSTLYDGHWFERLLSHLEQTDADLNSRLFHCNEKHGG
                     HVGEDKTFAYIVREYPLTHCEDKALVPVAALASPMPDDRLFLEHLAEHYYQQDTLAWFQ
                     DYVDLLCQVHLTLWLRYGIALESNQQNAIIAFDQQGKMTLAMKDNDAAEFGQSASTLQQ
                     SMLPKNTAQNYFQWIAMNCSINASKSTMNWRSVRCLPPSHYNWISQRL"
     gene            complement(6191..7390)
                     /gene="pvsA"
     CDS             complement(6191..7390)
                     /codon_start=1
                     /gene="pvsA"
                     /product="PvsA"
                     /protein_id="ABM30209.1"
                     /transl_table=11
                     /translation="MIIVSHVVNDAVTHGLVPAAKAMDLHVVLITDHKLSHLELASRDS
                     HFNPDTILECDVFNPLELIETITEHELKPDAIFSNSDHLQTSTAICAQFFGLPSKDWSV
                     TLKAKNKHLTRQVLNEKSLPNTQSCLLHRHSNVALGMAFPVVAKPKEGVASLDVQRCDS
                     QSELDAYCEAFWQKHPNAAILVEQFLQGPVVTLETLGDGNQLIAVGGFDVSLSEPPYFI
                     ETAADWNGPNGIAHREACLAQLKAFGVGFGVCHSEFIITENGPVLVEINYRSIGDGREF
                     LLNNLAHFGWFSTILGLHLGRRLDGDYQIHGSAHVHYVVAEHSGVIDGGSTSFIQHDDD
                     IITQQQVLKTAGEHFQQSYSNKDYLARISIVSPSGRNLDQSLQNALSNFNLAPTREVAA
                     "
     gene            7653..9431
                     /gene="psuA"
     CDS             7653..9431
                     /codon_start=1
                     /gene="psuA"
                     /gene_functions="transport (smcogs)
                     SMCOG1051:TonB-dependent siderophore receptor (Score:
                     427.7; E-value: 1.5e-129)"
                     /gene_kind="transport"
                     /note="putative receptor of exogenous siderophore"
                     /product="PsuA"
                     /protein_id="ABB02669.2"
                     /transl_table=11
                     /translation="MNKLLTLTPLAVAIGSSLVAPSAVASEETNSTPSATETIQVFGHQ
                     YEGYAEHMPQSGTKTDVEWLDVPQAVSVVTKTEMQDRGAVRLVDALDGVAGVNNTLGEG
                     SRDQFMIRGFDSLNDMYRDGLRDDGTLQSYRSLANVERVEIVKGPAGALYGRGSAGGII
                     NLVTKRANGENFTHVNGSVGSNNQYVGQVDSSMAFTDKVNGRINLEYRQADSYVDHVDS
                     NDFFIAPTIRVLPAEGHTIDIDVEYAHQELVPYRGVPSKNGKPVDLPVSTYFGGTNDYQ
                     ESDSLRIAVDYEWRLNDQWVWNNRAAFNHIELEQKGTRQGKVTGNEVSQTVNNFGYDPR
                     TTTTLQSELVWETRDNQLMVGADFNQIDIDLTLASDKTLPPQNIYDPVVGPTPDPGFKP
                     FRDNTTTTTGLYVQDVYTWGDLSLIGNVRYDSMELEQQKAGSDKEKLDDDKVSYRAGLV
                     YRINSDTSVYASLARSWQLPYAGIYINPKLAEFFHTDLKEVGAKAYLLDNALMLNAALF
                     QIDQEQPQTNVDGDVIDKIEVRHQGIELEARGQITKQWDISVGYSYLDAEDKATGKKPN
                     DVSDHLFSLWSTYQFG"
     gene            9813..11951
                     /gene="pvuA"
     CDS             9813..11951
                     /codon_start=1
                     /gene="pvuA"
                     /gene_functions="transport (smcogs)
                     SMCOG1051:TonB-dependent siderophore receptor (Score:
                     257.7; E-value: 3.8e-78)"
                     /gene_kind="transport"
                     /note="vibrioferrin receptor"
                     /product="PvuA"
                     /protein_id="ABB02670.1"
                     /transl_table=11
                     /translation="MSYQNLDGKVRVRAGLSTTYSVLAIAIASATSMSAVAAPAKTEQT
                     TAMETVVVTGSVIGKSEIEDVKEYPGARTVITREQIEKTAAGSIDNALQRVPGIKVQDE
                     SGTGVLPNISVRGLKASRSGHAQFLMDGVPLTLAPYGHTGQSIFPATLSMLDRIDIVRG
                     GAAVQYGPNNVGGVINLVTKPIPHTWQTEISNRLTVFDGGDAPLNDFYLRTGGWLSDTF
                     ALQLEGNFLKGESFREHSDTDVKNFQAKAQWLLSDTQEIQAFLQRYDAETQMPGALSPQ
                     DYEQDRHQSKRPYDDYEGKSTRWSVKYIHDLPFADSAELEVLTFGHKSERLFKWGFNSA
                     GGHWADPALPATDVRTSPREFTVYGIEPKMAMYFGEGKSVTQNWIVGTRYVNEDIDYKL
                     TQTPIAGGATSVPRDWHLDTDAFAGYVSNEIGLFNDTLKVTPGLRVESVRMTFTDRVKK
                     QTADNTVTEWLPGLTVAYNLTDQWVTYANAQKSLRAPQIAYIRGLGEEGSELAWNYEVG
                     ARYTQDATSFNAALYRIDFEDQLQWQSSTQTFDNIGKTLHQGIELSARYVPEALPALSL
                     GASYNYLDATLEEEGANKGNQLPYTSKHQLGWDATYAFYGMDTTLSGFYFSDSYTDNAN
                     TSAEDATGATGKVPSYMVWNFNLGTDLYKDDKGKLRMNVAVNNLFDEEYYFRGIDTSPV
                     GRYPAPGRSYTLDLNYQF"
     gene            12052..12975
                     /gene="pvuB"
     CDS             12052..12975
                     /codon_start=1
                     /gene="pvuB"
                     /gene_functions="transport (smcogs) SMCOG1033:iron compound
                     ABC transporter, periplasmic (Score: 195.9; E-value:
                     1.3e-59)"
                     /gene_kind="transport"
                     /note="ferric vibrioferrin-binding periplasmic protein"
                     /product="PvuB"
                     /protein_id="ABM30205.1"
                     /transl_table=11
                     /translation="MKNKKQFTIKHFSTVLLMLLSSALMSFSAFSQARSVQDEQGTFEL
                     EAIPQRIVVLEFSFVDALAAVDVSPVGVADDNDAIRVIPAVRAKIEPWQSVGMRSQPSL
                     EAIAVLKPDLIIADAERHRAIYQDLQRIAPTLLLKSRGETYQENLESAQKIGVAIGKQA
                     KMTQRIEQHKQTMAEFKQHFATQETIQFGVVSDKGMWLHSPVSYAGGVLSTLGIQSPLA
                     PSEQKAYIPTSFELLLRTNPDWLLVGLYSQPNIVDEWRKNPLFKLLTAAKKQQLVEVSP
                     ELWSLNRGMLAAEEIARNLEALLERS"
     gene            12972..14009
                     /gene="pvuC"
     CDS             12972..14009
                     /codon_start=1
                     /gene="pvuC"
                     /gene_functions="transport (smcogs) SMCOG1011:transport
                     system permease protein (Score: 268.7; E-value: 1e-81)"
                     /gene_kind="transport"
                     /note="ferric vibrioferrin transport system permease"
                     /product="PvuC"
                     /protein_id="ABM30206.1"
                     /transl_table=11
                     /translation="MSAAISSESASSVSKSHWKASLFVCAMALCTLSGYAASMIGWSNF
                     SLSVNDLTSYWFAFDEGNMLHQILATLRAPRTYAGLLIGASLAVSGVLMQGLTRNPLAS
                     PSILGINAGAACFMALASIGVPFFSQLNPIINAVLGALLSGGAVMLLGGFFSARSHPLR
                     LVLAGIAISALLIGLTRASVILADDMAYSVLHWLTGSLSAVDSEQWQQLWPFATLGLVL
                     AMGLARNLNLLALGDEVAVGLGGNIRLTRLISGLAVVLLAGTSVATAGPIGFVGLLVPH
                     LVRPIVGHNYHILIPVSALCGAALVTWSDALSRAIAFPAETPVGVITALLGTPCFIAIA
                     MRKSS"
     gene            14006..14977
                     /gene="pvuD"
     CDS             14006..14977
                     /codon_start=1
                     /gene="pvuD"
                     /gene_functions="transport (smcogs) SMCOG1011:transport
                     system permease protein (Score: 282; E-value: 9e-86)"
                     /gene_kind="transport"
                     /note="ferric vibrioferrin transport system permease"
                     /product="PvuD"
                     /protein_id="ABM30207.1"
                     /transl_table=11
                     /translation="MMNQTKLFALVGLLLIASSLTLFVGAANMSAQQVFALLFSFSDSD
                     FVIHQYRLPRMLLAIGVGAGLGLSGVLVQGVIRNPLASPDLMGISAGAGLAATACLVLY
                     PNAPVAMLPMVAMAGGLLAAGFIAVLAYWSKPTPARLALIGVAVSAFLASGIDFLLIVH
                     PIEINTAMVWLTGSLWGRNWQQVPFIWSALLLLLPLAFWLAWRLDVMGLGEESATTLGT
                     KPRQIQILALIAAVLLASISVSVAGTISFVGLLAPHLARLLFGHTHKLLIPAAATLGAL
                     LVICADGLARGLQPPIELPAGVLTSVIGAPYFIFLLYRYRGW"
     gene            14980..15744
                     /gene="pvuE"
     CDS             14980..15744
                     /codon_start=1
                     /gene="pvuE"
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 208.2; E-value:
                     2.4e-63)"
                     /gene_kind="transport"
                     /note="ferric vibrioferrin transport ATP-binding protein"
                     /product="PvuE"
                     /protein_id="ABM30208.1"
                     /transl_table=11
                     /translation="MLRTQNLSVAYGKQTIIPDLSLSIPQGKITALVGPNGCGKSTLLK
                     TLVRINKPASGQVLYNDKPLSSYGDKRLARSLSLLPQILVSPEGITVRKLVEYGRSPYL
                     SHWGRLSQQDKALVEQVMQDTGVIEFADKPVESLSGGQRQRAWIAMVMAQDTDVVMLDE
                     PTTYLDLSHQVELMKLMRKMNDKGKTVVVVLHDLNQACRYCDHLVVLEKGQLVTQGTPQ
                     EVLTEGMLASVFDLQARVFNDPISGTPMCVAV"
ORIGIN
        1 aaggctgtga cgaggtattg aacaaaatca gcggcgtgct gggcaattta acccacgcgg
       61 cggcgaagta gggtgcagta ggcaatgaat ttgtgccgag atggaataaa aaagccccaa
      121 tgattggggc ttgagtaaag cgaagtatgc cgtttaaaaa tagtggaaga tcggcggttc
      181 atgcatcaag aagttttgat gggagatgtt ccacgcatag gcgcccgcta aggtaaacac
      241 aacgtaatca ccaatatcga cgtgggcgac gtgttgattg cgtgccagca catctttggg
      301 cgtacaaagc tggccgacaa aggtcgcttg tgcatgctga attggatgag aaatctcatg
      361 gtgttgctcg cttttcagga tgacgaatgg atgatcatgg ctctgcgccg ctggcgtgcg
      421 gaagtggtga gttcctccgc gcgcgatgac aaagttttcg ccaagatttt gtttaatatc
      481 cagtacttcc atcacgtaat aaccgcatgc cgccgtgatg aaacgtccgc attcaaaacg
      541 cagtgtccag tcttgcactt gctctttggc gatgaggaat tccaacttgt cgcaaaactc
      601 catccacgga aagtgttgct cagggttttg gtagttaatc cccataccgc cgcccaagtt
      661 gatcatcagc tcactgagtt tgaactcctg ctgccacgtt ttcacgactt ggaaatagcg
      721 ctgcattaac gctaagtgac gttcaacatc gagctgatgc gacatcaagt gaaaatggaa
      781 gcctttgagt gacacttgcg ggaaatcgcg cagcaacatc aaagcattac tcagctcgga
      841 ctcgtccaag ccaaatggag tcggcttgcc acccatcgcc aacttgctga gcgtgatgtc
      901 gccaatatcg atgttcatac gcaaaaagat cgaagcaggg cggttgaggc gctcggttag
      961 tgctccaatg cgttgtagct cggtgaggct ttcgacatga aatgcatcaa catcaagctc
     1021 aatcgcttgt tgcaattcac ttagcatttt tccggggcca ccgaagatca atggcttatc
     1081 gagttgttgc tggtggaggt gtgtcaactc cccgccagat gccgcttcaa aaccatcgac
     1141 atacggcgcg agcgttttaa gaatcgggcc cgatggattg gctttggctg cgtaaaatag
     1201 ctcgacattt ctcggcaaaa cgtgacgcag ttgtttgatg tgttcttcta aggcgcttag
     1261 gtcatacacg tatgcgcaca agggttgact ttgctgctgt gcgagtgtgt gaacttgttg
     1321 gcaaacgagt gtcggtagag agagggaatt agacatgttg cgctcctttt tcgtgttgct
     1381 gccaaggcgt ggcgacttgg gtatagcccg actccttatc ggctttttgc atcaaacgcg
     1441 tggtgaaatt atttttgctc gggaagtgac cgccattcag caacgcttct agttccggct
     1501 gttttccatt cactgattgc caacgcacaa tggcactttg caccgcattc catagcgttt
     1561 gctctagtgg ctcgtcacct tctgcgatga agaaaatcgc ttcgctgatg ttgttgataa
     1621 aggtgcagta tccgatgcga ttccagccat gttcgcgact gtagtaaacc gattgtcgcg
     1681 cgcgttcaga caagtcagtt aaagtttctg ctggccagaa ctccggcagt aacttggtgc
     1741 cttccaaatc acgaatccaa acacaacacg gcatctcttg ctcaaagcca atcagggtgt
     1801 tttgcaaatg cggctcaaac gccaccccgt gtttgaagta gtaattaaat acgcccggga
     1861 tcaggcaatg caaatagcgt tcaaaccaaa gtgtcgcgat gtcggaatag cgactttgcg
     1921 tagtacgcgc tttgttttta aggcgttgag caatgcgact gttgccgtta ttatcgtaag
     1981 caaacagagc gccagcgagg gttggtttca agatgtcgac ttcgctcagc gagaaatttt
     2041 cgcgatacaa aatcccaaac gattcgcgtg ctttggtgtt gtcgtcccag ttgtgctcag
     2101 cgacactttc tagattcaac gtggtggcgt aaggctctgt catcacttta aatacggggt
     2161 tgtgcagctg ttcgttttct ctaattggtt tgagaatcga cgtgagttgc acggcgctgt
     2221 cgagttcgta ccacgcgttt ttgcgcacgc aattggttaa tcgtacgttg atggagaatt
     2281 tcgcaaacca gtccatgtca gggtggtaga gggtacgaac tgaagaggtc ggctgcatct
     2341 tttcaccacc taaacccagt ggcgtgatct tgccttgttc aatggcgcgc ttcacgcttg
     2401 ggttactcaa aatggtgtag ccttcccaag gatgacaagg gtagagggtc ttcccattgc
     2461 tggtggcgtg ttgtgccgcg agccgttgta gcatgcgctg cgcagggtta gcttctccgt
     2521 cggaactcag cacatcaagt aaggacgtat ccacttcaaa ccagtacaac gggacttgcg
     2581 cgccgacttc tggagaaaac gccaagagat catccatggt gacgcccgaa cgacttttcg
     2641 gggtcggatg gaaagtatgc ccccaaatca gagattgttc cgaagcgata aattcgttgt
     2701 gatgcgattt gggcgcgacg ttgagaaact gctcggtaac cagcacgcta ttttctagtt
     2761 gttcgatcag ctcatggtta aacgggatcg ccaatacctg cttgagatag ttaagcaaaa
     2821 gcgtaagagt ttgcgtggca tccaatttcg cccaaccaga tcccggcatt ttcgcccaag
     2881 gcagttctgc caatttgatt ttgcccagtt tgctcgcgta gtgaatcggc aaaaccagca
     2941 cgccattttg ttgagaaaga tgaacggcta tccgctgttt agcgggatag ttgcgtttta
     3001 acgtttgggg tagcgatgtc gattggtagt gccattccac ttgttgattc ggtagcgcaa
     3061 attcacgtaa gtaacaatta agaacaccca ttatcgtgtt gagttgggct ttctcacggt
     3121 tgagctgcac ctcatgcgcg cgcaactcgc tagtctgaga ggattgagtt tgtaacatgt
     3181 tgatttccaa attttgaagt ggagaaccag cgcgatcaag gtgattacgc tgactatcgc
     3241 agaaagtagg aaaggaacct caagtagccc caagctagaa atgaaagaag cgcaaaatcc
     3301 cgctgccacg cccccccatt tggacatggc atcaaagcga gaaaatactt tgccactgag
     3361 tttgcgatcc agattgctcg caagatgctg atgcaatccg tgataacaac acaccatcgc
     3421 gatgccgaag ataattctcg cagcaatgag tgcggtttcg gatgcaatca aatgcaaggc
     3481 agagctcacg gcaagcaatg caaacccaag ccacaacgtg cgcagcggtt gatgccgatt
     3541 cagcccatct gacaacacgt ggattttgcc tgccagcaac agatagacac catgcggcag
     3601 tgcgtacaaa aaaccgatcc atgcgtggtt agtgatgcca tattgctgca caaacggcga
     3661 gaaataagga aaggtcacca ccatagaaaa cgcaaacccg aactgagcaa agaagatcca
     3721 acgaatggca tgcattgcgt ttttctgtgc aaccggtttt tcttgctgag gttctgagtt
     3781 tggcttttcg aggttttggt cggcgggttt atccactggc agtgttagcg tgatgcccat
     3841 cgcaatcaaa ggcaaaacgc ataaccaaag gtacgcgttg agcgggttgg tctctgacaa
     3901 gctccagccg agcccaatag gcgcaacgat cagcgccaag cgagcagata gctgggtttt
     3961 attgagtgac ttgccgagtg ttttggcttc tttaatttga ctagataagt agctgttcga
     4021 ggcggccatg gtgccgccaa aggttccttg aacaataaga ccaaacacaa acatggcgag
     4081 gttgtctgca aagccgcaca gcgcaaaccc tgctgcaagg ccaagttgcg cgcgcaagag
     4141 tgagcggcgg cgaccgtagc gatcagcaaa gttgccccaa aatcgcgccg ccaacgcagt
     4201 acaaaacgtg ggtaggataa acaatacccc tgcccagtct gcatcaatgc ttgcgcctaa
     4261 cgttggcaac acgacgggta gaaatagcgg catacctaac gcggcaaacg cagcgataaa
     4321 gtggcacaac aacacgctgt agatgagttt ggtcataatc gtttaacgta cgcttttttt
     4381 gatggcttct aagtaggcgc gttgcgcttc tttgctagaa agcagcaaga agttcggagc
     4441 cgacaaacca tagaacttat tgatgtcact cgcgccggag gcttctttcg agagcaaact
     4501 gccagagctc aacaagtatt tggcgtacaa atcaggtgct tcttgcagta actcgcgtgc
     4561 cagttttgtc tctaaccctc cgctttctag tcggttgagt tgctctgcaa tgcttttggc
     4621 cacgatggcg taaagcgatg ctgaagaggc aatgcccttc gctgccatgg cttctacaat
     4681 cgctgcgata tccagttgta atgtgatggt ggtaaacatc tgaccgagcg ccaattcatt
     4741 gtcgactttg atgcgttgat cgagcagttc atcgcaatcc actggaagta gttttgcgcc
     4801 gtgtttttgg gcagcatgct ctgttgcaaa gtggaagcgc tctggccaaa ttccgccgcg
     4861 tcgttgtctt tcatcgcgag cgtcattttg ccctgttgat caaatgcaat gatggcgttt
     4921 tgctggttgg actccaatgc gatgccataa cgaagccaca aggtgaggtg cacttggcat
     4981 aatagatcga cgtaatcttg gaaccacgcc agcgtatctt gttgatagta atgttctgcc
     5041 aaatgttcta agaacaagcg gtcgtcaggc attgggcttg ctagtgctgc aaccggaact
     5101 aacgctttgt cttcgcaatg agtaagtgga tactcacgta cgatgtacgc gaaggtttta
     5161 tcctcgccga cgtgtccacc gtgtttttcg ttgcaatgga acaagcgact gtttaaatcg
     5221 gcgtccgttt gttcaaggtg gctgagtagg cgttcaaacc agtggccgtc atacagtgtt
     5281 gaaggcttga ttaagcgaat atttttcgtg cctagcgatc gcatgatcag cggaaccttg
     5341 atgtgaatgt gcggtgcgtc gttcacgacc accgtacgaa cggaaagcgt cggcgtcacg
     5401 tctagatacg ttaaaggcgc ttctatcata ccttcgggta aggcttctaa agatcgcagg
     5461 gtgagcggat gcgcagggaa cagtacgtgg ctcagtgcaa aatcagcagg aagcccaact
     5521 tgctccatgg ttggccagca atcaggttgt tcgctagtta atgtcaacag tgatttttca
     5581 atcgcaatcc aatgcagagc aaagctttgt gcgaactcgg gcgcgtactt agcaaggtct
     5641 tcatcgctca ggccaaactt cgcccgcgca gttggatagt acgggtggtc aaggtaagaa
     5701 gcgatctggt cggctagcag cagtttttga tgccaagaac caagcttgga tattggttga
     5761 gaaatgtggt ctgcgtgatg atgaaacgct tggtcacaca gcgctttgtg tttctctgca
     5821 cattccagtt cttgaaggta tcccgccagc aactcgtgag agctctcatg cgccagtgtc
     5881 gttagtagcg caatccactc tttgtaatgc tgttgatgct cgatttggcc atttttctcc
     5941 acccaccaac ctggtggtgt ttcgtcattg gcgtgaccgt agcgccagtg ctgcatgtaa
     6001 tacgctggcg caacaggtaa atacaacgtg aaatcagagt tggcaaatat cagccagact
     6061 tgctcgtctt gaggaacaat tacccctttt ggcgcgtctc tactgaactt ggccttcgac
     6121 aaaatgccaa acatgtcttc gcgtaagcaa gtgtcgatca atcgttgcgt tagatacaaa
     6181 gcgttgtcat tcatgctgcc acctcacgag tcggagctag attgaaattg ctaagcgcgt
     6241 tctgcagcga ttggtcgagg tttcgccctg atggtgagac aatcgaaatt cgggcgagat
     6301 aatccttatt ggaataactc tgttggaagt gttctccagc ggtttttagt acctgttgct
     6361 gagtgatgat atcgtcatcg tgttggatga acgacgtcga acctccgtcg atcacaccgc
     6421 tgtgttccgc aactacgtag tgcacgtgcg ctgacccatg aatttggtaa tcgccgtcca
     6481 atcttcgacc caaatgcaga cccaaaattg tgctaaacca accaaagtga gcgagattgt
     6541 tgagcaagaa ttcgcggcca tctccaatac tgcggtagtt gatttccacc agaactgggc
     6601 cgttttccgt gatgataaat tcgctgtggc aaactccaaa accaacgccg aacgctttca
     6661 gttgagccaa gcaagcttct cggtgagcaa tgccgtttgg tccgttccag tctgcggcgg
     6721 tttcgataaa gtaaggcggc tcagagagcg acacatcaaa accgcccacg gcgataagtt
     6781 ggttaccgtc tcctaaagtt tcgagcgtaa ccacaggtcc ttgaagaaac tgctccacca
     6841 gaatggcggc gtttgggtgc ttttgccaaa acgcctcgca gtaagcatcg agctcgcttt
     6901 gggagtcaca gcgctgtaca tctagacttg ccacgccttc tttgggtttt gctacgacag
     6961 ggaaggccat gcctaatgcc acattcgaat ggcgatgcag caaacagctt tgtgtgttgg
     7021 gtaaggattt ttcatttaac acttgtcgag ttaagtgttt gtttttggcc tttaacgtga
     7081 cagaccaatc tttggatggg aggccaaaaa attgcgcgca gatggcggtg gaagtttgta
     7141 agtgatcact attgctgaaa atagcgtctg gctttaattc atgctccgtg atggtttcga
     7201 tgagttctaa tgggttgaag acatcacatt ctagaatggt atccggatta aagtgactat
     7261 cacgactcgc caactctaaa tggctgagct tatggtcggt gataagaacg acgtgaaggt
     7321 ccatcgcttt agctgcaggc actaagccgt gagtgactgc atcgttaacg acatgactta
     7381 cgataatcat cgtagatttc atgagctttc ctaattttct aacattttct tctaacgttg
     7441 ataaagtcgc tcacggtcgt cttccaatgt gcatctctcg agacggaggg cagaagtttc
     7501 agtatttatt gttatgatat ttggtttttt ttgttgttag ttgtgttttt agggtaaata
     7561 aatatagagc ataaaattaa tattgcaaat agaaataata tttgttctta tttccattcg
     7621 ttggctttat aacaactaaa cggaattatg gaatgaataa attattgacc ctgaccccct
     7681 tggcggtcgc gattggttct tcattagtag caccaagcgc agtggcttca gaagagacga
     7741 actcaacccc atcagcaact gaaacgattc aagtcttcgg tcatcagtac gaaggttatg
     7801 ctgaacacat gcctcaatct ggcaccaaaa ccgatgtgga atggcttgat gtgccacaag
     7861 cggtatccgt agtaacgaaa acagaaatgc aagaccgagg cgcggtgcgt ttagtggatg
     7921 cgctagatgg ggtagccggg gtcaataaca ccttgggcga aggcagccgc gatcagttca
     7981 tgatccgtgg ttttgattca ctcaacgata tgtaccgtga tggcttgcga gatgacggta
     8041 cgttgcagtc ttaccgcagc ttagcgaacg ttgaacgcgt tgaaattgtg aaaggccctg
     8101 cgggtgcttt gtatggccgt ggttcggctg ggggcatcat caacttggtg acgaaacgcg
     8161 ctaacggtga gaattttact cacgtaaacg gtagtgtagg cagcaacaac caatatgttg
     8221 gtcaggtcga cagctcaatg gcgtttacag ataaggtcaa cgggcgtatt aacttggaat
     8281 accgtcaagc cgactcgtac gttgatcatg ttgattcaaa cgatttcttc attgcaccga
     8341 ccattcgtgt gttaccagcg gaaggtcaca ccattgatat tgacgtggaa tacgcccatc
     8401 aagaactagt gccgtatcgt ggtgtgccat caaaaaatgg caagccagtt gatttgccag
     8461 taagcactta ctttggcggc acaaacgact atcaggagtc agacagtctc cgtattgcgg
     8521 tggattacga atggcgtctg aatgatcaat gggtatggaa caaccgagcg gcgttcaacc
     8581 atatcgaact tgagcaaaaa ggcacgcgtc agggcaaagt gacgggtaat gaagtgtctc
     8641 aaaccgtcaa caactttggt tacgatccac gtactacgac cactttacaa tctgagttgg
     8701 tttgggaaac cagggacaat caattgatgg tgggtgcaga tttcaaccag atcgatatcg
     8761 acctaacgtt ggcaagtgac aaaacgcttc ctccgcaaaa tatctacgac ccagtcgttg
     8821 gtccaacgcc tgatccgggc tttaagccat ttcgtgacaa taccaccacc acgacaggtt
     8881 tgtatgtgca agacgtctat acgtggggtg acctttcttt gatcggtaac gtgcgttacg
     8941 actcgatgga actcgaacaa caaaaagcgg gctcagataa ggaaaaattg gatgatgaca
     9001 aagtcagcta ccgtgctggt ttggtatacc gcatcaattc cgacacatcc gtatacgcca
     9061 gtttagcgcg ctcttggcag ttaccgtacg ctggtatcta catcaatcca aaattggcgg
     9121 aattcttcca caccgactta aaagaggtgg gtgcgaaagc gtaccttttg gataacgcat
     9181 taatgttgaa tgcggcactg ttccagattg atcaagagca accacaaact aacgtggacg
     9241 gcgatgtgat cgataaaatt gaagtgcgtc accaaggtat tgaattagag gctcgcggtc
     9301 aaatcaccaa acagtgggac atctctgtgg gttacagcta cttggacgcg gaagacaaag
     9361 ccacaggcaa aaagccgaac gatgtatcag accacttgtt ctctctttgg agcacttacc
     9421 aatttggatg acaactggcg tttaggcggt ggtgtgaagt acgtgggcga ccgttacgca
     9481 ggtaacgacg aagctgtcgc acttggcgat tacacaacgg tggatttgat ggcggcgtac
     9541 accactggtc gtcataaaat ccaagcgaac gcttacaaca ttttggatga gaaatacatt
     9601 cttggcgcaa caaacggcac gtctggctta aaccagattg gctacggcgc accagccgag
     9661 tttatgctca gctacggata tcagttctag gctttttgtg cgtagttagc gcctgttagc
     9721 caatttttta cctcatattg aggatgtttt agccccgaga gcttacggga tgtattctgc
     9781 ccgaggctca atttctcgca aaggagatct taatgtctta ccagaatttg gatggtaaag
     9841 tgcgtgtacg tgctggtttg tctaccactt acagtgtgct tgcgattgca atcgcttctg
     9901 ccacatccat gagcgcggtt gccgctcctg ctaaaacaga acaaactacc gcgatggaaa
     9961 ccgtcgttgt cactggtagc gtgattggta aatctgaaat tgaagacgtc aaagagtacc
    10021 caggtgctcg taccgtcatt acccgcgagc aaatcgagaa aaccgccgca ggctcgattg
    10081 ataacgcgct acagcgcgtg ccgggtatta aagttcagga tgaaagcgga acgggcgtat
    10141 tgcccaacat ttccgtacgt ggtttgaaag caagccgtag tggtcatgct cagttcttga
    10201 tggatggtgt accgctaaca cttgcgccat atggtcacac tggtcagtct atcttcccag
    10261 caacactgtc gatgcttgat cgtattgaca ttgtacgcgg tggtgctgca gtacagtacg
    10321 gcccaaacaa cgtcggtggt gtgatcaact tagtgaccaa accgattcca cacacgtggc
    10381 aaactgaaat cagtaaccgc cttaccgtgt ttgatggcgg tgatgcgccg ctgaatgatt
    10441 tttacctacg cacgggcggc tggttaagcg acacatttgc actacagttg gaaggtaact
    10501 tcctaaaagg tgaaagcttc cgtgaacatt ccgatacgga cgtgaagaac tttcaagcta
    10561 aagcacagtg gctattgagt gacactcaag agattcaagc attcctgcaa cgttacgatg
    10621 ctgaaacgca aatgccgggc gcactttcgc cacaagacta cgaacaagat cgtcaccaat
    10681 cgaaacgtcc ttacgatgat tatgaaggta agtcgacacg ctggagtgtt aagtacatcc
    10741 acgatttgcc atttgccgac agtgccgagc tagaagtgct aacgtttggc cataagtcag
    10801 agcgtctttt caagtggggc tttaacagcg cgggtggcca ttgggcggat ccggctctgc
    10861 cagcgacgga cgttcgtacc tctccacgtg aatttaccgt gtatggtata gagccaaaaa
    10921 tggcgatgta ctttggcgaa ggtaaatccg ttactcaaaa ctggatcgtg ggtacccgtt
    10981 atgttaatga agacattgac tacaaactca ctcagactcc aatcgccggt ggtgcaacca
    11041 gcgttccgcg tgattggcat ctagatacgg acgcgtttgc tggatacgtt agcaacgaaa
    11101 ttggtttgtt caatgataca ttgaaggtaa cgcctgggtt gcgtgttgag tcggttcgaa
    11161 tgacgtttac cgatcgtgtt aaaaaacaaa cggcagacaa caccgtgacg gaatggttgc
    11221 caggtttgac ggtggcatac aacttaactg accagtgggt gacgtatgcg aacgcgcaaa
    11281 aatcacttcg tgcgccacaa attgcctaca tccgaggttt gggtgaagaa ggtagtgaac
    11341 tggcttggaa ctacgaagtc ggcgcgcgtt acacccaaga tgcgaccagc tttaacgctg
    11401 cactttatcg catcgacttt gaagatcaat tgcaatggca aagctcaacg cagaccttcg
    11461 ataacatcgg aaaaacactt caccaaggca tagagctttc tgctcgttac gtgcctgaag
    11521 cactacctgc actgagttta ggcgcaagct acaactatct agacgctacg cttgaagaag
    11581 aaggcgcgaa taaaggtaat caactgcctt acacctctaa gcatcagctt ggttgggatg
    11641 caacctacgc attttacggc atggacacca cgctatcggg cttctacttt agtgattcgt
    11701 ataccgataa cgccaacacc agtgccgaag atgcaactgg cgcaacgggt aaagtaccat
    11761 cttacatggt gtggaacttc aacttgggga cggatttata caaagacgac aaaggcaagt
    11821 tacgcatgaa tgttgcggtg aacaacttgt ttgacgagga gtattacttc cgcgggattg
    11881 atacaagccc agttggtcgg tacccagctc cgggacgctc ttacacactg gatctgaact
    11941 atcagtttta agcgtttcgt tcaccgagtt tgaaaagaaa agtcgccaat ttaccttggc
    12001 gactttgccg atcttaggta atggaaaact cttagcactg gaaaaagcaa gatgaaaaat
    12061 aaaaaacaat ttacgatcaa acacttttca accgttttgc tgatgctgtt gagctcagct
    12121 ttgatgtcat ttagcgcatt ttctcaggcg cgcagcgtgc aagatgagca aggcactttt
    12181 gagctagaag cgatacctca acgtattgtg gttttagaat tttcgtttgt cgatgcatta
    12241 gcggcagtcg atgtctcacc tgtaggcgtc gccgatgaca acgatgccat acgagtcatc
    12301 cctgctgttc gtgccaagat tgaaccttgg caatcggtcg gcatgcgctc acaaccgagt
    12361 ttagaggcga ttgcggtgct caagccggat ttgatcattg ccgatgccga acgtcatcgc
    12421 gcgatttatc aagatttgca gcgaattgcg ccaactttgc tgttaaaaag ccgtggagag
    12481 acatatcaag aaaatctcga atccgcacaa aaaatcggcg tggcaattgg caagcaagcg
    12541 aaaatgacac aacgaattga gcagcacaaa cagacaatgg cggagttcaa acagcatttt
    12601 gcgacacaag aaaccattca gtttggtgtg gtttctgaca aagggatgtg gctgcatagc
    12661 ccggtttctt acgctggggg cgttttgagt acgctgggca tccaaagccc gctggctcca
    12721 tctgagcaaa aagcgtatat cccaaccagt ttcgaacttt tactaagaac caaccctgat
    12781 tggttgttag ttggacttta ctctcaacca aatattgtgg atgagtggcg caaaaaccca
    12841 ttatttaagc tgctaaccgc tgcgaaaaaa cagcaacttg ttgaggtatc tcctgaactg
    12901 tggtcgttaa atcgcggcat gttggctgcg gaagaaattg cccgcaatct tgaagcgtta
    12961 ttggagcgct catgagtgcg gcgataagct cggaaagcgc gtcttcggtt agcaaatcgc
    13021 attggaaagc gagcttgttt gtctgtgcta tggcgttgtg tacgctctca ggctatgcag
    13081 cctcaatgat tggctggtcc aacttttctt tgagcgttaa tgatctgaca agctattggt
    13141 ttgcgtttga tgagggcaat atgctgcatc aaatcctcgc gacgctgcgt gcgcctcgta
    13201 cctatgctgg gttattaatt ggcgcttcgc ttgcggtatc cggagtgctg atgcaaggat
    13261 tgactcgtaa ccctttggcg tcgccatcca ttctcggcat taatgcaggt gcggcgtgtt
    13321 ttatggcctt ggcgtccatc ggcgtaccat tctttagtca attgaaccca atcatcaacg
    13381 cagtactcgg cgcgctacta agtggtggtg cggtcatgtt gttgggcggc tttttctccg
    13441 cgcgttctca cccattgcgt ttagtgctag cgggcattgc catcagtgca ctgctgatag
    13501 ggttaactcg tgcgtcggta attttggcgg atgacatggc ttacagcgtg ttgcattggt
    13561 taacgggttc actctccgcg gtagattctg agcagtggca gcaactgtgg ccttttgcga
    13621 ctttaggttt ggtgctcgcc atgggcttag cgcgaaactt aaatttactc gcgttggggg
    13681 acgaagttgc tgtcgggttg ggtggtaaca ttcgtcttac gcgattaatc agtggtttag
    13741 cggttgtgct gttggcagga actagcgtag cgactgctgg accaattggc tttgtcgggt
    13801 tgttagtgcc gcacttggta cgcccgattg ttggtcacaa ctatcacatt ctcattccgg
    13861 tttccgcctt gtgtggcgcg gcattggtca cgtggtcaga tgctttatca cgcgcgattg
    13921 cctttccggc tgaaacgcca gtgggcgtga ttaccgcgct gttgggcaca ccttgcttta
    13981 tcgcgattgc aatgaggaaa tcatcatgat gaatcaaacc aaactgtttg cattagttgg
    14041 attgttgctg attgcgtctt ctctcacgct gtttgttggc gcggcgaaca tgtcggctca
    14101 acaggttttt gcattgctgt tttctttctc tgactcggac tttgtgattc accaatatcg
    14161 attaccgaga atgttgctcg ccattggcgt gggcgctggg ttggggctgt caggcgtttt
    14221 agttcaaggc gtcatccgta atccacttgc ctcaccagat ttaatgggga taagcgccgg
    14281 agcaggactg gccgctaccg cttgtttggt gctgtatccg aatgcgccag tggcaatgtt
    14341 gccgatggtc gccatggcgg gaggcttgct tgctgctggc tttattgctg tgctggctta
    14401 ctggtcgaaa ccaacaccag cacgtttggc gctgattggt gttgctgtca gcgcattttt
    14461 agccagcggt atcgattttc ttctgattgt gcaccctatc gaaatcaaca cggcgatggt
    14521 atggctaacc ggcagtttgt ggggacgaaa ttggcaacaa gtgccgttta tttggagtgc
    14581 tctattgctg ctactaccat tggcgttttg gctggcttgg cggctagatg ttatgggact
    14641 gggagaagaa agcgccacta cacttggcac gaaaccaaga cagatccaaa ttcttgcgtt
    14701 gattgctgcc gtgctattgg cgagtatcag tgtgtctgtg gcaggcacga tcagctttgt
    14761 tggcctgctt gcgccgcatc ttgcgcgttt gctgtttggc cacacccaca agttgttgat
    14821 tccagccgct gcaaccctgg gcgctttgtt ggtgatttgc gccgatggtt tagcgagagg
    14881 cttacagcca ccaattgagc taccagcggg agtgctgaca tctgttattg gcgcaccata
    14941 tttcattttt cttttgtatc gttatagagg ttggtaagca tgttacgaac gcagaactta
    15001 tccgtcgcgt acggcaaaca gaccatcatt ccagatctct ctttgtcgat tcctcaaggt
    15061 aaaattaccg cgcttgttgg cccaaatggt tgcggtaaat cgacactatt aaaaaccttg
    15121 gtacgcatca ataaaccagc ttctggccaa gtgttgtata acgacaagcc actaagcagc
    15181 tacggtgata aacgcttggc acgctcgttg tctttgctgc cgcaaattct tgtcagtcct
    15241 gaaggtatta cagtcagaaa actggtcgaa tatggccgct cgccttatct ctctcactgg
    15301 gggcggttaa gtcagcaaga taaagcgttg gttgagcaag taatgcaaga tactggcgtg
    15361 attgagtttg ctgacaagcc agttgaatcg ttgtctggcg gtcaaaggca aagggcgtgg
    15421 attgcgatgg taatggcgca agataccgac gtagtgatgc ttgatgagcc aaccacttac
    15481 ttggatctct ctcaccaagt tgagctgatg aaacttatga ggaagatgaa cgacaaaggc
    15541 aaaacagtgg tcgtagtgct gcacgatcta aaccaagcct gccgttactg cgatcatttg
    15601 gtggtgttgg aaaaaggtca gttagtcacg cagggaacac cgcaagaggt actgacagaa
    15661 ggtatgttgg catcggtgtt tgatttacaa gcgagagtat ttaacgaccc aatctcaggc
    15721 acgccgatgt gtgtggcggt gtaatttacg tctttcgaaa gctatctgca ataaaaacgt
    15781 cgatccataa aaacaaaccc cgctagtaac aaagtcgcta gcggggtttg ttgtttttaa
    15841 gagcgtcctt aatgtggcgt ttaagccagt ttaaacgttg ccactttcga gttgagatcc
    15901 tcagcttgcg atttcagctc agcagattga ttcatcgatt cttcggcacc aactaccaag
    15961 tgatcggtca catctttaat ggacgtgacg ttttgcgtga tttcattggt tacgtgcgtt
    16021 tgctcttcgg cggctgtcgc aatttgcgtt gccatatcgc tgatcagcgc aacggccgcg
    16081 ttgatttcat ccagagccag tgctgcgcga tcagcatctt cc
//
