BGC0001017: microcystin LR biosynthetic gene cluster from Microcystis aeruginosa PCC 7806
Shows the layout of the region, marking coding sequences and areas of interest. Clicking a gene will select it and show any relevant details. Clicking an area feature (e.g. a candidate cluster) will select all coding sequences within that area. Double clicking an area feature will zoom to that area. Multiple genes and area features can be selected by clicking them while holding the Ctrl key.
View antiSMASH-generated output
Download Cluster GenBank file
Download region SVG
Location: 1 - 63,626 nt. (total: 63,626 nt).
This entry is originally from NCBI GenBank AF183408.1.

Legend:

core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
reset zoomreset view
zoomzoom to selection
Gene details
Shows details of the most recently selected gene, including names, products, location, and other annotations.
Select a gene to view the details available for it
General
Compounds
Genes
Biosynthesis
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0001017
Short description microcystin LR biosynthetic gene cluster from Microcystis aeruginosa PCC 7806
Status Quality: questionable
The quality level of this entry.

Status: active
The status of this entry.

Completeness: complete
Whether the entry covers everything needed for the pathway producing the compound(s)
Remarks "This cyclic peptide is an inhibitor of eukaryotic protein phosphatases type 1 and 2A. Synthesized nonribosomally by NRPS, PKS and hybrid PKS-NRPS enzymes, the microcystins contain a number of unusual amino acid residues, including the L-amino polyketide moiety Adda (3-amino-9-methoxy-2,6,8-trimethyl-10-phenyl-4,6-decadienoic acid)."
Biosynthetic class(es)
  • NRPS (Type I)
  • PKS (Type I)
Loci
AF183408.1
via Knock-out studies
Compounds
  • microcystin LR
Species Microcystis aeruginosa PCC 7806 [taxonomy]
References
Chemical products information
microcystin LR Evidence:
Copy SMILES
C49H74N10O12
Chemical database entries
PubCHEM
ChEBI
ChEMBL
ChemSpider
NPAtlas
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • AAF00953.1
  • dnaN
 3 - 422 (+) DNA polymerase III beta subunit
    copy AA seq
    copy Nt seq
    • AAF00954.1
    • mcyJ
    		 992 - 1828 (-) mcyJ
    • Tailoring
    • Knock-out
    copy AA seq
    copy Nt seq
    • AAF00955.1
    • mcyI
    		 2004 - 3017 (-) mcyI
    • Tailoring
    • Activity assay
    copy AA seq
    copy Nt seq
    • AAF00956.1
    • mcyH
    		 3056 - 4672 (-) mcyH
    • Transport
    • Knock-out
    copy AA seq
    copy Nt seq
    • AAF00957.1
    • mcyG
    		 4896 - 12791 (-) mcyG
      copy AA seq
      copy Nt seq
      • AAF00963.1
      • mcyF
      		 12923 - 13678 (-) mcyF
        copy AA seq
        copy Nt seq
        • AAF00958.1
        • mcyE
        		 13710 - 24173 (-) mcyE
          copy AA seq
          copy Nt seq
          • AAF00959.1
          • mcyD
          		 24340 - 36060 (-) mcyD
          • Scaffold biosynthesis
          • Knock-out
          copy AA seq
          copy Nt seq
          • AAF00960.1
          • mcyA
          		 36793 - 45180 (+) mcyA
          • Scaffold biosynthesis
          • Knock-out
          copy AA seq
          copy Nt seq
          • AAF00961.1
          • mcyB
          		 45196 - 51576 (+) mcyB
          • Scaffold biosynthesis
          • Knock-out
          copy AA seq
          copy Nt seq
          • AAF00962.1
          • mcyC
          		 51573 - 55448 (+) mcyC
            copy AA seq
            copy Nt seq
            • AAF00964.1
            • uma1
            		 55541 - 57592 (-) uma1
              copy AA seq
              copy Nt seq
              • AAF00965.1
              • uma2
              		 57836 - 58399 (-) uma2
                copy AA seq
                copy Nt seq
                • AAF00966.1
                • uma3
                		 58446 - 59972 (-) uma3
                  copy AA seq
                  copy Nt seq
                  • AAF00967.1
                  • uma4
                  		 60057 - 61271 (-) uma4
                    copy AA seq
                    copy Nt seq
                    • AAF00968.1
                    • uma5
                    		 62180 - 62614 (-) uma5
                      copy AA seq
                      copy Nt seq
                      • AAF00969.1
                      • uma6
                      		 62656 - >63624 (-) uma6
                        copy AA seq
                        copy Nt seq
                        Biosynthesis information

                        Biosynthetic modules

                        Name
                        0
                        Type
                        nrps-type1
                        Genes
                        mcyG
                        Substrates
                        cinnamic acid (evidence: Structure-based inference, Homology)
                        Integrated Monomers
                        Domains
                        adenylation
                        Name
                        3
                        Type
                        nrps-type1
                        Genes
                        mcyE
                        Substrates
                        D-glutamic acid branched (evidence: Structure-based inference [1], [3], [4])
                        Integrated Monomers
                        Domains
                        condensation (LCL), adenylation
                        Name
                        6
                        Type
                        nrps-type1
                        Genes
                        mcyA
                        Substrates
                        serine (evidence: Structure-based inference [5], [6])
                        Integrated Monomers
                        Domains
                        adenylation, methyltransferase
                        Name
                        7
                        Type
                        nrps-type1
                        Genes
                        mcyA
                        Substrates
                        alanine (evidence: Structure-based inference [1], [2])
                        Integrated Monomers
                        Domains
                        condensation (DCL), adenylation, epimerase
                        Name
                        8
                        Type
                        nrps-type1
                        Genes
                        mcyB
                        Substrates
                        leucine, valine, tyrosine, isoleucine, glycine (evidence: ATP-PPi exchange assay [2])
                        Integrated Monomers
                        Domains
                        condensation (DCL), adenylation
                        Name
                        9
                        Type
                        nrps-type1
                        Genes
                        mcyB
                        Substrates
                        3S-methyl-D-aspartic acid branched, D-aspartic acid branched (evidence: Structure-based inference, Feeding study)
                        Integrated Monomers
                        Domains
                        condensation (LCL), adenylation
                        Name
                        10
                        Type
                        nrps-type1
                        Genes
                        mcyC
                        Substrates
                        valine, leucine, isoleucine, arginine, glycine, lysine (evidence: ATP-PPi exchange assay [2])
                        Integrated Monomers
                        Domains
                        condensation (LCL), adenylation
                        Name
                        1
                        Type
                        pks-modular
                        Genes
                        mcyG
                        Substrates
                        Integrated Monomers
                        Domains
                        acyltransferase, ketosynthase, acyltransferase, ketoreductase, methyltransferase, carrier (ACP)
                        Name
                        2
                        Type
                        pks-modular
                        Genes
                        mcyE
                        Substrates
                        Integrated Monomers
                        Domains
                        acyltransferase, ketosynthase, acyltransferase, methyltransferase
                        Name
                        5
                        Type
                        pks-trans-at
                        Genes
                        mcyD
                        Substrates
                        Integrated Monomers
                        Domains
                        ketosynthase, dehydratase, ketoreductase, methyltransferase, carrier (ACP)
                        Name
                        6
                        Type
                        pks-modular
                        Genes
                        mcyD
                        Substrates
                        Integrated Monomers
                        Domains
                        acyltransferase, ketosynthase, acyltransferase, dehydratase, ketoreductase, carrier (ACP)
                        Annotation changelog

                        Entry version: 4

                        Date
                        Changes
                        Submitters
                        Reviewers
                        2022-10-07
                        Update chemical activity to schema version 2.11
                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                        Entry version: 3

                        Date
                        Changes
                        Submitters
                        Reviewers
                        2022-09-15
                        Linked new NPAtlas id for microcystin LR (npatlas:NPA0593)
                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                        2022-09-15
                        Corrected NRP module activity
                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                        2022-09-15
                        Removed ketoreductase stereochemistry annotation from modules without ketoreductases
                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                        2022-09-15
                        Updated NRP substrate specificities
                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                        Entry version: 2

                        Date
                        Changes
                        Submitters
                        Reviewers
                        2019-10-16
                        Migrated from v1.4
                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                        Entry version: 1

                        Date
                        Changes
                        Submitters
                        Reviewers
                        2015-06-12
                        Submitted
                        • (ID: EI7LXM427LE4WIOQ5U54V3BB)
                        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                        Detailed domain annotation
                        Shows NRPS- and PKS-related domains for each feature that contains them. Click on each domain for more information about the domain's location, consensus monomer prediction, and other details.
                        A domain glossary is available here, and an explanation of the visualisation is available here.
                        Selected features only
                        Show module domains
                        Similar known gene clusters from MIBiG 4.0
                        Shows clusters from the MiBIG database that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
                        Click on reference genes to show details of similarities to genes within the current region.
                        Click on an accession to open that entry in the MiBIG database.