BGC0001058: zorbamycin biosynthetic gene cluster from Streptomyces flavoviridis
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Location: 1 - 84,773 nt. (total: 84,773 nt).
This entry is originally from NCBI GenBank EU670723.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
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Gene details
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General
Compounds
Genes
Biosynthesis
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0001058
Short description zorbamycin biosynthetic gene cluster from Streptomyces flavoviridis
Status Quality: questionable
The quality level of this entry.

Status: active
The status of this entry.

Completeness: complete
Whether the entry covers everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • NRPS (Type I)
  • PKS (Type I)
  • saccharide (hybrid/tailoring)
Loci
EU670723.1
via Knock-out studies
Compounds
  • zorbamycin
Species Streptomyces flavoviridis [taxonomy]
References
Chemical products information
zorbamycin Evidence:
Copy SMILES
C55H85N19O21S2
Chemical database entries
NPAtlas
PubCHEM
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • ACG60741.1
 34 - 345 (-) hypothetical protein
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    • ACG60740.1
    		 395 - 799 (-) conserved hypothetical protein
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      • ACG60739.1
      		 851 - 2347 (-) probable DEAD-box RNA helicase
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        • ACG60738.1
        		 2697 - 2900 (-) putative cold shock protein
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          • ACG60737.1
          		 3993 - 4481 (-) small oligopeptide transporter
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            • ACG60736.1
            		 4849 - 5601 (+) putative thioesterase
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              • ACG60742.1
              		 5714 - 6343 (+) hypothetical protein
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                • ACG60743.1
                		 6340 - 7320 (+) putative dehydrogenase
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                  • ACG60744.1
                  		 7317 - 8147 (+) putative hydrolase
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                    • ACG60745.1
                    		 8223 - 10724 (+) acylase
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                      • ACG60763.1
                      • zbmA
                      		 10945 - 11343 (+) zbm binding protein
                      • Resistance/immunity
                      • Other in vivo study
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                      • ACG60780.1
                      • zbmVIId
                      		 11346 - 12155 (+) acyltransferase
                      • Scaffold biosynthesis
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                      • ACG60779.1
                      • zbmVIIc
                      		 12106 - 13356 (-) cytochrome P450
                      • Precursor biosynthesis
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                      • ACG60782.1
                      • zbmX
                      		 13584 - 19979 (+) NRPS(C/A/PCP/C/A/PCP)
                      • Scaffold biosynthesis
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                      • ACG60773.1
                      • zbmIX
                      		 19976 - 23293 (+) NRPS(C/A/PCP)
                      • Scaffold biosynthesis
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                      • ACG60781.1
                      • zbmVIII
                      		 23290 - 28725 (+) PKS(KS/AT/MT/KR/ACP)
                      • Scaffold biosynthesis
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                      • ACG60777.1
                      • zbmVIIa
                      		 28722 - 30653 (+) NRPS(C/PCP)
                      • Scaffold biosynthesis
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                      • ACG60776.1
                      • zbmVI
                      		 30650 - 38794 (+) NRPS(AL/ACP/C/A/PCP/C/A)
                      • Scaffold biosynthesis
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                      • ACG60775.1
                      • zbmV
                      		 38791 - 40584 (+) NRPS(PCP/C')
                      • Scaffold biosynthesis
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                      • ACG60768.1
                      • zbmG
                      		 40581 - 41543 (+) NAD-dependent sugar epimerase
                      • Precursor biosynthesis
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                      • ACG60767.1
                      • zbmF
                      		 41536 - 43092 (+) glycosyl transferase and hydroxylase
                      • Tailoring
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                      • ACG60746.1
                      		 43097 - 45010 (+) Gln-dependent amino transferase
                      • Scaffold biosynthesis
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                      • ACG60772.1
                      • zbmIV
                      		 45003 - 52922 (+) NRPS(C/A/PCP/Cy/A/PCP/Cy)
                      • Scaffold biosynthesis
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                      • ACG60771.1
                      • zbmIII
                      		 52903 - 55446 (+) NRPS(A/PCP/Ox)
                      • Scaffold biosynthesis
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                      • ACG60747.1
                      		 55443 - 56588 (+) oxygenase
                      • Tailoring
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                      • ACG60770.1
                      • zbmII
                      		 56585 - 57997 (+) NRPS(C)
                      • Scaffold biosynthesis
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                      • ACG60748.1
                      		 57994 - 58605 (+) Unknown
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                        • ACG60766.1
                        • zbmE
                        		 58602 - 59762 (+) glycosyl transferase
                        • Tailoring
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                        • ACG60765.1
                        • zbmD
                        		 59759 - 61387 (+) carbamoyltransferase
                        • Precursor biosynthesis
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                        • ACG60769.1
                        • zbmI
                        		 61384 - 61650 (+) type II PCP
                        • Scaffold biosynthesis
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                        • ACG60764.1
                        • zbmC
                        		 61647 - 63140 (+) NTP-sugar synthase
                        • Precursor biosynthesis
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                        • ACG60749.1
                        		 63137 - 64411 (+) SAM-dependent oxidase or methyl transferase
                        • Scaffold biosynthesis
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                        • ACG60750.1
                        		 64408 - 65028 (+) ankyrin-like protein
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                          • ACG60783.1
                          • zbmXI
                          		 65090 - 66439 (+) NRPS(C)
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                            • ACG60778.1
                            • zbmVIIb
                            		 66458 - 68233 (-) NRPS(A/PCP)
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                              • ACG60751.1
                              		 68515 - 69459 (+) alpha-ketoglutarate-dependent hydroxylase
                              • Tailoring
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                              • ACG60752.1
                              		 69551 - 71380 (+) NRPS(C)
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                                • ACG60753.1
                                		 71377 - 72648 (+) type II PCP
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                                  • ACG60754.1
                                  		 72645 - 72911 (+) type II PCP
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                                    • ACG60755.1
                                    		 72914 - 74512 (+) acyl-CoA synthetase
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                                      • ACG60756.1
                                      		 74509 - 75288 (+) NRPS(TE)
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                                        • ACG60757.1
                                        		 75325 - 75933 (+) ABC transporter
                                        • Transport
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                                        • ACG60758.1
                                        		 75940 - 76620 (+) ABC transporter
                                        • Transport
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                                        • ACG60759.1
                                        		 76614 - 77213 (+) ABC transporter
                                        • Transport
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                                        • ACG60760.1
                                        		 77936 - 79132 (+) biotin synthase
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                                          • ACG60774.1
                                          • zbmL
                                          		 79218 - 80231 (-) GDP-mannose-4,6-dehydratase
                                          • Precursor biosynthesis
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                                          • ACG60761.1
                                          		 80243 - 83659 (+) NRPS(C/A)
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                                            • ACG60762.1
                                            		 83596 - >84771 (-) amidohydrolase
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                                              Biosynthesis information

                                              Biosynthetic modules

                                              Name
                                              4
                                              Type
                                              nrps-type1
                                              Genes
                                              zbmX
                                              Substrates
                                              asparagine (evidence: Sequence-based prediction)
                                              Integrated Monomers
                                              Domains
                                              condensation (LCL), adenylation
                                              Name
                                              5
                                              Type
                                              nrps-type1
                                              Genes
                                              zbmX
                                              Substrates
                                              histidine (evidence: Sequence-based prediction)
                                              Integrated Monomers
                                              Domains
                                              condensation (DCL), adenylation
                                              Name
                                              6
                                              Type
                                              nrps-type1
                                              Genes
                                              zbmIX
                                              Substrates
                                              homoserine (evidence: Sequence-based prediction)
                                              Integrated Monomers
                                              Domains
                                              condensation (LCL), adenylation
                                              Name
                                              2
                                              Type
                                              nrps-type1
                                              Genes
                                              zbmVI
                                              Substrates
                                              serine (evidence: Sequence-based prediction)
                                              Integrated Monomers
                                              Domains
                                              condensation (LCL), adenylation
                                              Name
                                              3
                                              Type
                                              nrps-type1
                                              Genes
                                              zbmVI zbmV
                                              Substrates
                                              asparagine (evidence: Sequence-based prediction)
                                              Integrated Monomers
                                              Domains
                                              condensation (DCL), adenylation
                                              Name
                                              9
                                              Type
                                              nrps-type1
                                              Genes
                                              zbmIV
                                              Substrates
                                              beta-alanine (evidence: Structure-based inference, Homology)
                                              Integrated Monomers
                                              Domains
                                              condensation (LCL), adenylation
                                              Name
                                              10
                                              Type
                                              nrps-type1
                                              Genes
                                              zbmIV
                                              Substrates
                                              cysteine (evidence: Structure-based inference, Homology)
                                              Integrated Monomers
                                              Domains
                                              condensation (Heterocyclization), adenylation, oxidase
                                              Name
                                              8
                                              Type
                                              nrps-type1
                                              Genes
                                              zbmVIIb
                                              Substrates
                                              valine (evidence: Sequence-based prediction)
                                              Integrated Monomers
                                              Domains
                                              adenylation
                                              Name
                                              1
                                              Type
                                              pks-trans-at-starter
                                              Genes
                                              zbmVI
                                              Substrates
                                              Integrated Monomers
                                              Domains
                                              ligase, carrier (ACP)
                                              Name
                                              7
                                              Type
                                              pks-modular
                                              Genes
                                              zbmVIII
                                              Substrates
                                              Integrated Monomers
                                              Domains
                                              acyltransferase, ketosynthase, acyltransferase, ketoreductase, methyltransferase, carrier (ACP)
                                              Annotation changelog

                                              Entry version: 4

                                              Date
                                              Changes
                                              Submitters
                                              Reviewers
                                              2022-10-07
                                              Update chemical activity to schema version 2.11
                                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                                              Entry version: 3

                                              Date
                                              Changes
                                              Submitters
                                              Reviewers
                                              2022-09-15
                                              Corrected NRP module activity
                                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                              2022-09-15
                                              Removed ketoreductase stereochemistry annotation from modules without ketoreductases
                                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                              2022-09-15
                                              Sorted modules by module number
                                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                              2022-09-15
                                              Corrected NRP module numbers
                                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                              2022-09-15
                                              Updated NRP substrate specificities
                                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                                              Entry version: 2

                                              Date
                                              Changes
                                              Submitters
                                              Reviewers
                                              2019-10-16
                                              Migrated from v1.4
                                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                              2019-10-16
                                              Updated compound(s) information (NPAtlas curation)
                                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                                              Entry version: 1

                                              Date
                                              Changes
                                              Submitters
                                              Reviewers
                                              2015-06-12
                                              Submitted
                                              • (ID: E2T2HCM4X7QXVS4LL4JVBPPK)
                                              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                              Detailed domain annotation
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