LOCUS       BGC0001244              5358 bp    DNA     linear   PLN 10-FEB-2015
DEFINITION  Parastagonospora nodorum strain SN15 mellein synthase (mlnS) gene,
            complete cds.
ACCESSION   BGC0001244
VERSION     BGC0001244
KEYWORDS    .
SOURCE      Parastagonospora nodorum
  ORGANISM  Parastagonospora nodorum
            Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina;
            Dothideomycetes; Pleosporomycetidae; Pleosporales; Pleosporineae;
            Phaeosphaeriaceae; Parastagonospora.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-08 00:14:59
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5358
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..5358
                     /db_xref="taxon:13684"
                     /mol_type="genomic DNA"
                     /organism="Parastagonospora nodorum"
                     /strain="SN15"
     gene            <1..>5358
                     /gene="mlnS"
                     /locus_tag="SNOG_00477"
     protocluster    1..5358
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[0:5358]"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1..5358
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
     cand_cluster    1..5358
                     /SMILES="C([*])C(-O)"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(PKS_AT and (PKS_KS or ene_KS or
                     mod_KS or hyb_KS or itr_KS or tra_KS))"
                     /kind="single"
                     /product="T1PKS"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..5358
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0001244"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..5358
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T1PKS"
                     /region_number="1"
                     /rules="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS))"
                     /subregion_numbers="1"
                     /tool="antismash"
     CDS             1..5358
                     /EC_number="2.3.1.-"
                     /NRPS_PKS="Domain: PKS_KS(Iterative-KS) (41-465). E-value:
                     3.1e-160. Score: 525.5. Matches aSDomain:
                     nrpspksdomains_SNOG_00477_PKS_KS.1"
                     /NRPS_PKS="Domain: PKS_AT (576-867). E-value: 9.6e-72.
                     Score: 233.7. Matches aSDomain:
                     nrpspksdomains_SNOG_00477_PKS_AT.1"
                     /NRPS_PKS="Domain: PKS_DH (934-1045). E-value: 8.8e-10.
                     Score: 31.0. Matches aSDomain:
                     nrpspksdomains_SNOG_00477_PKS_DH.1"
                     /NRPS_PKS="Domain: PKS_KR (1417-1609). E-value: 8.7e-54.
                     Score: 174.0. Matches aSDomain:
                     nrpspksdomains_SNOG_00477_PKS_KR.1"
                     /NRPS_PKS="Domain: PKS_PP (1701-1777). E-value: 6e-18.
                     Score: 56.9. Matches aSDomain:
                     nrpspksdomains_SNOG_00477_PKS_PP.1"
                     /codon_start=1
                     /gene="mlnS"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_AT"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_KS"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1093:Beta-ketoacyl synthase (Score: 115.3; E-value:
                     4.5e-35)"
                     /gene_kind="biosynthetic"
                     /locus_tag="SNOG_00477"
                     /note="polyketide synthase producing (R)-mellein; MLNS"
                     /product="mellein synthase"
                     /protein_id="AIW00670.1"
                     /sec_met_domain="PP-binding (E-value: 7.5e-16, bitscore:
                     44.8, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="PKS_KS (E-value: 5.6e-162, bitscore:
                     525.5, seeds: 2284, tool: rule-based-clusters)"
                     /sec_met_domain="PKS_AT (E-value: 1.8e-73, bitscore: 233.7,
                     seeds: 1682, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short (E-value: 8.9e-49, bitscore:
                     152.0, seeds: 230, tool: rule-based-clusters)"
                     /transl_table=1
                     /translation="MATPDDPATPALSLSASNSSSPTAASSVPPPTGTSEIQYDDVAII
                     GMSCRTAGGNDSPEKLWRFIMDKKDASGESPSWRWEPWVRRDTRNAKVIEKTISKGYFI
                     EDLENFDASFFGISPKEAEQMDPHQRLGLEVTWEALEDAGINPQSLSGSDTAVYVGVDS
                     DDYSRLLLEDIPNIEAWMGIGTTAHGIPNRISYHLDLMGPSAAVDAACASSMVAVHTGR
                     QAILAGESRIAIVGGVNVCLSPALFHMLGAAGALSPDGVCLSFDEEARGYARGEGAAIL
                     ILKKMSHAIMDGDHILATIKGSAIAQDGKTNGIMAPNAKAQELVARKALKQAGINALTV
                     GYIEAHATSTPLGDPTEVSAISAVYGVGRPTDTPALIGSIKPNVGHLEAAAGAISLVKA
                     VMAVQKGIVPPQTRLNKLNTRVDWAKSGLHVVRESTQWGTEDSPRRAAICSYGYGGTVS
                     HAIIEQFAHAADPFTASTSDDNHPTLLLLSAPQGKQRLPAQSAALAEWISPAGAHESLR
                     SIAATLATRRAHHENRAAFIVSSHTEAAETLNLFSKGAEHDSIVQSRTLDNNINKQIVW
                     VFSGHGSHWSGMGKQLLQNAVFYRTVAPLDIVVVQELGYSAIEALKTGRFESSGQVQVL
                     TYMTQIGLIQLLKAKGVHPHAVIGHSVGEIAASVAAGCLTPEEGMIIVTRRARLFAKVI
                     GCGGMFLVSLPFAEVLAELGGRTDIVAAIDSSPSSCVISGLNAPLEEYVEKLKNRGIRV
                     FQVKTDIAFHSPMLEVLSKPLKESLEGSLNPQPPNIKLYSTSQADTRHPARRDAEYWVD
                     NMVKPVWLRPAVTAAIEDHYRIFMEVSTHPIVSHSLDETLAENGASDFTTIHTMKKEQS
                     AEKCILHAVAQLWTKGVKIDFKFLGRQWSREVPKIRWSHKRFWKEVSTGSASAQTVHDP
                     DKNNMLGQRMVVAGTNMTIFTTALDESSKPFPMPHQLHGTDIIPVSVYVNTFIKATGGK
                     VLSKMELRVPLAVTNDVRNVQVIVDGQSVKVASRLSSSDDMSWVTHSTASWENEPSANV
                     LPSLDVSSVIKRIGTRVSETFSVDYLKKTGVSGMAFPWAVNDHYNNTKEMLVTLDNDPE
                     HETMSWDPCSWGATLDAATSVGATLFSREVKLRIVSHIDRLTIYSSDPPPKRYHLYVTE
                     ASSSQVHACSADISVLDLSGTLLARIESIRFTEVEATPTKSTSIESGVHQIAWVPARLS
                     EKPLSLEQIVLVSEDDAKLEQYANELQRQAPKIVKLTSAAKLRDNGALFMREKNATVIY
                     CPGTVTSLEDVASASHRFIWEVATAIKFLVENSISAKFFVILDRTFLAGSPTALAQGAL
                     YGLARVVASEHSDIWGGLIDNEGPLFPVMPLKYVQDQDITRYIDGVPRVARMRPFTKQQ
                     RYAPSTARTLLPKPEGTYVLTGGLGALGLETCDFLIEKGARRIVVISRRDIPARSQWSK
                     ASENLAPILERVKAMEKTGASIYFVSLDIGAADAHQQLLFALERLSLPPVLGVIHASGV
                     LEDSLLVDTTSDSFARVLSPKISGALALHKAFPPGTLDFFVLYSSIGQLVGTSGQSSYA
                     AGNSFLDVLAAHRRSQGDNAIAFQWTAWRGLGMATSTDFLTLELQSKGITDVGRDEAFQ
                     AWEHMSKYDVDQAVVTRTLALEADDILPCALLEEVVVRKARAQDQSAPASGNASDSSGR
                     PTASADLKPWLDVKIRECVALVMGVEDIEEIDTRVPLSDYGVDSIMTIALRQKLQSKLK
                     IKVPQTLMWNYPTVSAMVGWFQKQFEEGQ"
     CDS_motif       28..72
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_SNOG_00477_0001"
                     /evalue="9.90E+01"
                     /label="PKSI-KS_m3"
                     /locus_tag="SNOG_00477"
                     /protein_end="24"
                     /protein_start="9"
                     /score="-3.7"
                     /tool="antismash"
                     /translation="PALSLSASNSSSPTA"
     aSDomain        124..1395
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_SNOG_00477_PKS_KS.1"
                     /domain_subtype="Iterative-KS"
                     /evalue="3.10E-160"
                     /label="SNOG_00477_PKS_KS.1"
                     /locus_tag="SNOG_00477"
                     /protein_end="465"
                     /protein_start="41"
                     /score="525.5"
                     /tool="antismash"
                     /translation="VAIIGMSCRTAGGNDSPEKLWRFIMDKKDASGESPSWRWEPWVRR
                     DTRNAKVIEKTISKGYFIEDLENFDASFFGISPKEAEQMDPHQRLGLEVTWEALEDAGI
                     NPQSLSGSDTAVYVGVDSDDYSRLLLEDIPNIEAWMGIGTTAHGIPNRISYHLDLMGPS
                     AAVDAACASSMVAVHTGRQAILAGESRIAIVGGVNVCLSPALFHMLGAAGALSPDGVCL
                     SFDEEARGYARGEGAAILILKKMSHAIMDGDHILATIKGSAIAQDGKTNGIMAPNAKAQ
                     ELVARKALKQAGINALTVGYIEAHATSTPLGDPTEVSAISAVYGVGRPTDTPALIGSIK
                     PNVGHLEAAAGAISLVKAVMAVQKGIVPPQTRLNKLNTRVDWAKSGLHVVRESTQWGTE
                     DSPRRAAICSYGYGGTVSHAIIEQF"
     aSModule        124..5331
                     /complete
                     /domains="nrpspksdomains_SNOG_00477_PKS_KS.1"
                     /domains="nrpspksdomains_SNOG_00477_PKS_AT.1"
                     /domains="nrpspksdomains_SNOG_00477_PKS_DH.1"
                     /domains="nrpspksdomains_SNOG_00477_PKS_KR.1"
                     /domains="nrpspksdomains_SNOG_00477_PKS_PP.1"
                     /iterative
                     /locus_tags="SNOG_00477"
                     /monomer_pairings="pk -> pk"
                     /tool="antismash"
                     /type="pks"
     CDS_motif       604..651
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_SNOG_00477_0002"
                     /evalue="1.70E-04"
                     /label="PKSI-KS_m3"
                     /locus_tag="SNOG_00477"
                     /protein_end="217"
                     /protein_start="201"
                     /score="14.0"
                     /tool="antismash"
                     /translation="GPSAAVDAACASSMVA"
     CDS_motif       793..831
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_SNOG_00477_0003"
                     /evalue="2.90E-03"
                     /label="PKSI-KS_m4"
                     /locus_tag="SNOG_00477"
                     /protein_end="277"
                     /protein_start="264"
                     /score="10.9"
                     /tool="antismash"
                     /translation="FDEEARGYARGEG"
     CDS_motif       1024..1071
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_SNOG_00477_0004"
                     /evalue="6.40E-03"
                     /label="PKSI-KS_m5"
                     /locus_tag="SNOG_00477"
                     /protein_end="357"
                     /protein_start="341"
                     /score="9.4"
                     /tool="antismash"
                     /translation="YIEAHATSTPLGDPTE"
     CDS_motif       1132..1176
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_SNOG_00477_0005"
                     /evalue="1.50E-03"
                     /label="PKSI-KS_m6"
                     /locus_tag="SNOG_00477"
                     /protein_end="392"
                     /protein_start="377"
                     /score="11.1"
                     /tool="antismash"
                     /translation="GSIKPNVGHLEAAAG"
     CDS_motif       1723..1779
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_SNOG_00477_0006"
                     /evalue="3.10E-05"
                     /label="PKSI-AT-mM_m1"
                     /locus_tag="SNOG_00477"
                     /protein_end="593"
                     /protein_start="574"
                     /score="16.4"
                     /tool="antismash"
                     /translation="VWVFSGHGSHWSGMGKQLL"
     aSDomain        1729..2601
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_SNOG_00477_PKS_AT.1"
                     /evalue="9.60E-72"
                     /label="SNOG_00477_PKS_AT.1"
                     /locus_tag="SNOG_00477"
                     /protein_end="867"
                     /protein_start="576"
                     /score="233.7"
                     /specificity="consensus: pk"
                     /specificity="PKS signature: Malonyl-CoA"
                     /specificity="Minowa: mmal"
                     /tool="antismash"
                     /translation="VFSGHGSHWSGMGKQLLQNAVFYRTVAPLDIVVVQELGYSAIEAL
                     KTGRFESSGQVQVLTYMTQIGLIQLLKAKGVHPHAVIGHSVGEIAASVAAGCLTPEEGM
                     IIVTRRARLFAKVIGCGGMFLVSLPFAEVLAELGGRTDIVAAIDSSPSSCVISGLNAPL
                     EEYVEKLKNRGIRVFQVKTDIAFHSPMLEVLSKPLKESLEGSLNPQPPNIKLYSTSQAD
                     TRHPARRDAEYWVDNMVKPVWLRPAVTAAIEDHYRIFMEVSTHPIVSHSLDETLAENGA
                     SDFTTIHTMK"
     CDS_motif       1951..2070
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_SNOG_00477_0007"
                     /evalue="1.20E-16"
                     /label="PKSI-AT-M_m3"
                     /locus_tag="SNOG_00477"
                     /protein_end="690"
                     /protein_start="650"
                     /score="52.8"
                     /tool="antismash"
                     /translation="GVHPHAVIGHSVGEIAASVAAGCLTPEEGMIIVTRRARLF"
     aSDomain        2803..3135
                     /aSDomain="PKS_DH"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_SNOG_00477_PKS_DH.1"
                     /evalue="8.80E-10"
                     /label="SNOG_00477_PKS_DH.1"
                     /locus_tag="SNOG_00477"
                     /protein_end="1045"
                     /protein_start="934"
                     /score="31.0"
                     /tool="antismash"
                     /translation="MLGQRMVVAGTNMTIFTTALDESSKPFPMPHQLHGTDIIPVSVYV
                     NTFIKATGGKVLSKMELRVPLAVTNDVRNVQVIVDGQSVKVASRLSSSDDMSWVTHSTA
                     SWENEPS"
     aSDomain        4252..4827
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_SNOG_00477_PKS_KR.1"
                     /evalue="8.70E-54"
                     /label="SNOG_00477_PKS_KR.1"
                     /locus_tag="SNOG_00477"
                     /protein_end="1609"
                     /protein_start="1417"
                     /score="174.0"
                     /specificity="KR activity: active"
                     /specificity="KR stereochemistry: (unknown)"
                     /tool="antismash"
                     /translation="GTYVLTGGLGALGLETCDFLIEKGARRIVVISRRDIPARSQWSKA
                     SENLAPILERVKAMEKTGASIYFVSLDIGAADAHQQLLFALERLSLPPVLGVIHASGVL
                     EDSLLVDTTSDSFARVLSPKISGALALHKAFPPGTLDFFVLYSSIGQLVGTSGQSSYAA
                     GNSFLDVLAAHRRSQGDNAIAFQWTAWRG"
     CDS_motif       4255..4353
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_SNOG_00477_0008"
                     /evalue="2.40E-15"
                     /label="PKSI-KR_m1"
                     /locus_tag="SNOG_00477"
                     /protein_end="1451"
                     /protein_start="1418"
                     /score="49.0"
                     /tool="antismash"
                     /translation="TYVLTGGLGALGLETCDFLIEKGARRIVVISRR"
     CDS_motif       4669..4779
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_SNOG_00477_0009"
                     /evalue="9.00E-20"
                     /label="PKSI-KR_m4"
                     /locus_tag="SNOG_00477"
                     /protein_end="1593"
                     /protein_start="1556"
                     /score="63.3"
                     /tool="antismash"
                     /translation="LDFFVLYSSIGQLVGTSGQSSYAAGNSFLDVLAAHRR"
     aSDomain        5104..5331
                     /aSDomain="PKS_PP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_SNOG_00477_PKS_PP.1"
                     /evalue="6.00E-18"
                     /label="SNOG_00477_PKS_PP.1"
                     /locus_tag="SNOG_00477"
                     /protein_end="1777"
                     /protein_start="1701"
                     /score="56.9"
                     /tool="antismash"
                     /translation="ADLKPWLDVKIRECVALVMGVEDIEEIDTRVPLSDYGVDSIMTIA
                     LRQKLQSKLKIKVPQTLMWNYPTVSAMVGWF"
ORIGIN
        1 atggcgaccc cggatgaccc agcgacgcca gccttgtctc tatctgcatc taattcttcg
       61 tctccaacag ctgcatcttc cgtcccgccg cccaccggga cttctgaaat ccaatatgac
      121 gatgtagcaa tcattggaat gtcatgccgc acagctggtg gcaatgactc gcccgagaaa
      181 ctatggcggt tcataatgga caagaaggat gcgtcgggag agagcccgag ctggcgctgg
      241 gaaccatggg tgcgacgaga cactcgcaac gcaaaagtca tcgagaagac catctccaaa
      301 ggctacttca ttgaggatct tgagaacttt gacgcatcct tctttggtat ttcacccaag
      361 gaggccgagc agatggatcc tcaccaaaga cttggcctgg aagttacgtg ggaagctctc
      421 gaagacgctg gaattaaccc acagtcactc tcagggtctg acactgctgt ctacgttggt
      481 gttgactcgg atgattactc cagacttcta ctcgaagaca tacccaacat cgaggcgtgg
      541 atggggatcg gaaccacagc gcatggcatt cctaacagaa tctcatacca tcttgaccta
      601 atgggcccca gtgctgcggt agatgcggcg tgtgcttcat ccatggtcgc cgtccataca
      661 gggagacaag ccattctcgc aggagaatcg cggattgcaa ttgtgggtgg agtgaatgtt
      721 tgcttgtccc cggcattatt ccacatgcta ggagcagctg gtgcactctc tccggatggt
      781 gtctgccttt cctttgatga ggaagcccgc ggttacgcta gaggagaagg tgctgcaatc
      841 ctcatcctca agaaaatgtc gcatgcgatc atggacggag atcacattct tgccaccata
      901 aaaggaagcg ccatcgctca ggacggaaag acaaatggca tcatggctcc aaatgccaaa
      961 gcgcaggagc tggtggccag gaaggccctc aaacaagcgg gtattaatgc tttgacagta
     1021 ggctacatcg aagctcatgc cacatcgacc cctttgggtg atcccacgga agtatctgct
     1081 atctctgcag tatatggagt gggccgcccc acagataccc cagccctcat cggatctatc
     1141 aagccaaacg ttggccatct cgaggctgcc gctggtgcca tcagtctcgt gaaggcagtg
     1201 atggcggtcc agaaagggat cgttccccca cagacaagac ttaacaaact caacaccagg
     1261 gtggattggg cgaagtccgg tctgcatgtg gttcgagaga gcacgcagtg gggcacagaa
     1321 gatagtcctc gtcgtgctgc catctgttct tacggatacg gcggaacggt gagtcatgcc
     1381 atcatcgagc agtttgcaca tgccgcagac ccatttacag cctcaacgag tgacgataac
     1441 caccccactc tcctgttact ctccgcaccg cagggaaaac aaagactgcc agcgcagagt
     1501 gcagctttgg ctgagtggat ctcaccagcc ggcgcacacg aaagccttag atctatcgca
     1561 gcaaccttgg caacacgcag agctcatcac gaaaaccgtg ccgcttttat cgtctctagt
     1621 cacacagagg ctgccgagac gctcaatctc ttctccaaag gagcagaaca tgattcaatt
     1681 gtacaaagcc gcaccctgga caataacatc aacaagcaaa ttgtctgggt tttctcgggg
     1741 catggaagtc attggtcagg catgggcaag caacttcttc aaaatgcggt tttctaccgt
     1801 actgtggcgc cactggacat tgttgtggta caggaactag gctattctgc aattgaggcc
     1861 ctcaagacgg gcagatttga aagttcgggc caagtccagg tcttaacata tatgacgcaa
     1921 attggtttga ttcaactcct caaggccaag ggcgtccatc cccatgcggt aatcggacat
     1981 tccgttggcg agatcgctgc gtcagtagcc gccggctgcc ttactccgga ggagggaatg
     2041 atcatagtca caaggcgtgc acgcctcttc gccaaagtta tcggttgcgg aggcatgttc
     2101 ctcgtgagtc tgccgtttgc tgaggtcctc gcagagctag gtggcaggac tgatatagtg
     2161 gccgctattg actcatcacc ttcatcctgt gtcatcagtg gcctcaatgc gcccctggaa
     2221 gaatacgtcg agaagttaaa aaatcgaggt atcagggtgt tccaggtcaa aacagacata
     2281 gcctttcaca gtcccatgct tgaggttcta tcaaagcctt tgaaagaaag cttagaaggt
     2341 tcactaaacc cacagccgcc aaatatcaag ctgtattcga catcccaggc ggacactcga
     2401 cacccagctc gcagagatgc agaatattgg gttgacaaca tggtcaagcc agtatggctt
     2461 cgacctgccg tgaccgcagc tatcgaagac cattacagga tcttcatgga agtctcaacc
     2521 catccaatcg tgtcacattc tcttgatgag accttggctg agaatggggc gagcgacttc
     2581 actacaatcc atacgatgaa gaaagagcaa tctgccgaaa aatgcattct tcatgctgtc
     2641 gcacaactct ggactaaggg cgtcaagatc gacttcaagt tccttggtcg gcagtggagt
     2701 agagaggttc ccaagattcg atggagccac aagcgattct ggaaagaggt gtcgacaggc
     2761 tctgctagtg ctcaaactgt acacgatcct gacaagaaca acatgcttgg ccagcggatg
     2821 gttgtcgccg gaactaatat gacaatcttc accaccgctc ttgacgaaag cagcaagcca
     2881 ttcccgatgc cacaccagct tcacggaacg gatatcatcc cggtctcagt ctacgtcaac
     2941 acttttatca aagcgactgg tggcaaagta ctctctaaga tggagcttcg cgtacctctc
     3001 gcggtcacga acgatgtccg caatgttcag gtcattgtcg atggacagag tgtcaaggtt
     3061 gcttcacgtc ttagctcttc agacgatatg tcatgggtga cgcacagtac tgcatcttgg
     3121 gagaatgaac caagtgcaaa cgtactacca tctctggacg tatcatccgt tatcaaacga
     3181 attggtaccc gcgtctcgga aacattctcg gtagactacc tcaagaagac tggcgtgtca
     3241 gggatggcat ttccatgggc cgtcaacgac cattataaca acactaaaga gatgcttgta
     3301 acgctcgaca acgaccctga acatgagaca atgtcatggg atccttgttc atggggtgct
     3361 acacttgacg ctgcgacttc agtcggtgct actttgttct cacgcgaagt caaactacgc
     3421 attgtctcgc acatcgatag gttgaccatc tattcgtccg atccaccgcc gaaaagatat
     3481 cacctttacg taaccgaagc aagcagcagc caagtccatg cttgttccgc ggatatctct
     3541 gtacttgacc tgagtggtac actcctggcc agaatcgagt caatcaggtt cacggaggtt
     3601 gaagccacac caacgaagag cacaagcatt gagagtgggg tacatcagat tgcatgggtt
     3661 ccagcccgtc tgtccgagaa gcctctgtca ctggaacaaa tcgtacttgt gtctgaagac
     3721 gatgcaaaac ttgagcaata tgcgaatgag ttgcagcgac aggctccaaa aatcgtgaag
     3781 ttgacctcgg ccgccaaact tcgtgataat ggggcgctgt tcatgcgtga gaagaacgcg
     3841 accgtaatct attgtccagg tacagttaca tcgctcgagg atgttgcaag cgcatctcat
     3901 cgatttatct gggaggttgc aactgcgatc aaattccttg tcgagaacag catttctgca
     3961 aaattctttg taattcttga tcgcaccttc ttggctggat cacctaccgc gctggcccaa
     4021 ggtgctttgt atggtcttgc tcgcgtcgtc gcctcggagc attctgacat ttggggtgga
     4081 ctcatcgaca acgaaggccc gcttttcccg gtgatgccac tcaagtatgt gcaagaccag
     4141 gatatcacac gttacatcga cggagtccca agagttgcac gaatgaggcc attcacaaag
     4201 caacaacgct atgcgccttc gaccgctcga actcttctgc ccaagcccga aggtacatat
     4261 gtcctcaccg gtggactagg tgctcttggt cttgagactt gcgacttcct tattgagaag
     4321 ggtgcacgtc gtatcgtcgt aatctcgcgg cgtgatatac ccgctcgtag ccagtggtca
     4381 aaagcttctg aaaatttagc gccaattttg gaaagagtga aggccatgga gaagacgggt
     4441 gctagcattt actttgtttc ccttgacatt ggcgctgcag atgcgcatca acaactcctt
     4501 tttgcactcg agcgactttc actaccaccc gtcctcggtg tcattcacgc aagtggtgtg
     4561 ctcgaagaca gcttgctagt ggataccacc tcagactctt tcgcacgagt actctcgccc
     4621 aagatctccg gcgccctcgc actgcacaaa gctttccctc ctggcactct cgactttttc
     4681 gtgctctact cttcaatagg acagctcgtc ggaacatctg gacagagttc atatgctgcc
     4741 ggcaactctt tcctcgacgt cctggcagca catcgtcgga gtcagggcga caatgcaatt
     4801 gctttccaat ggacagcttg gcgcggccta ggtatggcca cgtctaccga cttcctgacc
     4861 ttggagctgc agagcaaagg aataactgat gtcggtcgcg atgaggcttt ccaagcgtgg
     4921 gagcacatgt ccaagtacga cgtcgatcaa gctgtcgtta cgcgtacctt ggcgcttgaa
     4981 gcagatgaca ttctcccatg tgcgcttctc gaagaagttg ttgttcgaaa agcacgcgcg
     5041 caagatcaga gtgcacctgc atctggcaat gcgagcgaca gcagtggtcg cccaacagcg
     5101 agcgcagatc tcaagccttg gcttgatgtc aaaattcgag agtgcgtcgc cttggtcatg
     5161 ggcgttgaag acatcgagga gatcgatacc cgtgtgccct tatcggatta cggtgttgac
     5221 agcatcatga ctatcgctct tagacaaaag ttgcagagca aactcaagat caaggtaccc
     5281 caaacattaa tgtggaatta tccaactgtt agtgctatgg tgggatggtt tcagaagcag
     5341 tttgaggaag gccagtaa
//
