BGC0001331 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0001331 (Salinispora arenicola CNR107)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	T1PKS,NRPS-like	1	34,976	BE-43547A1 / BE-43547A2 / BE-43547B1 / BE-43547B2 / BE-43547B3 / BE-43547C1 / BE-43547C2	NRP:Cyclic depsipeptide + Polyketide:Modular type I	70%

BGC0001331 - Region 1 - NRPS-like,T1PKS

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Location: 1 - 34,976 nt. (total: 34,976 nt) Show pHMM detection rules used

Region on contig edge.

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

External annotation

Name		MIBiG
Version		3.0

Type		Subregion
Location		1 - 34,977
Label		BGC0001331

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001331.1	1.38	NRP, Polyketide	BE-43547A1, BE-43547A2, BE-43547B1, BE-43547B2, BE-43547B3, BE-43547C1, BE-43547C2	Salinispora arenicola CNR107
BGC0001162.1	0.77	Polyketide	1-heptadecene	Moorea producens JHB
BGC0001164.1	0.67	Polyketide	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001330.1	0.66	NRP, Polyketide	BE-43547A1, BE-43547A2, BE-43547B1, BE-43547B2, BE-43547B3, BE-43547C1, BE-43547C2	Micromonospora sp. RV43
BGC0000041.1	0.63	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0001163.1	0.63	Polyketide	1-heptadecene	Moorea producens 3L
BGC0001161.1	0.63	Polyketide	1-heptadecene	Moorea bouillonii PNG
BGC0001833.1	0.57	NRP	icosalide A, icosalide B	Burkholderia gladioli
BGC0001132.1	0.57	NRP	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002057.1	0.57	Polyketide	pateamine	Kiritimatiellaeota bacterium

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001331.1	0.94	NRP, Polyketide	BE-43547A1, BE-43547A2, BE-43547B1, BE-43547B2, BE-43547B3, BE-43547C1, BE-43547C2	Salinispora arenicola CNR107
BGC0001162.1	0.71	Polyketide	1-heptadecene	Moorea producens JHB
BGC0001330.1	0.70	NRP, Polyketide	BE-43547A1, BE-43547A2, BE-43547B1, BE-43547B2, BE-43547B3, BE-43547C1, BE-43547C2	Micromonospora sp. RV43
BGC0001164.1	0.67	Polyketide	1-heptadecene	Cyanothece sp. PCC 7822
BGC0000041.1	0.65	Polyketide	oronofacic acid	Pseudomonas syringae
BGC0001163.1	0.65	Polyketide	1-heptadecene	Moorea producens 3L
BGC0001161.1	0.65	Polyketide	1-heptadecene	Moorea bouillonii PNG
BGC0000389.1	0.64	NRP	massetolide A	Pseudomonas fluorescens SS101
BGC0001132.1	0.63	NRP	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833.1	0.62	NRP	icosalide A, icosalide B	Burkholderia gladioli

Similar known gene clusters

Module view

Legend

NRPS/PKS products

NRPS/PKS monomers

Predicted core structure(s)

For candidate cluster 1, location 0 - 34976:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Me-ohmal) + (mal) + (gly - ser) + (ccmal) + (NMe-gly)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS monomer predictions

F583_RS01000000127205

Search NORINE for peptide: strict or relaxed

AMP-binding (487..877): gly

NRPSPredictor2: gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: gly, ala
Single AA prediction:: gly

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGMIWK
Nearest Stachelhaus code:: gly
Stachelhaus code match:: 100% (strong)

F583_RS0124830

PKS_AT (530..817): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 87.5%; inactive: 79.2%; 2-Rhyd-Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 155.7
inactive: 76.9
Methoxymalonyl-CoA: 55.6
Methylmalonyl-CoA: 51.2
Propionyl-CoA: 29.5
2-Methylbutyryl-CoA: 27.1
Isobutyryl-CoA: 23.6
Ethylmalonyl-CoA: 19.6
Benzoyl-CoA: 18.9
CHC-CoA: 18.3
Acetyl-CoA: 17.5
fatty_acid: 14.7
trans-1,2-CPDA: 0.0
3-Methylbutyryl-CoA: 0.0

F583_RS01000000127210

Search NORINE for peptide: strict or relaxed

AMP-binding (484..873): gly

NRPSPredictor2: gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: gly, ala, val, leu, ile, abu, iva
Small clusters prediction:: gly, ala
Single AA prediction:: gly

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGMvWK
Nearest Stachelhaus code:: gly
Stachelhaus code match:: 90% (moderate)

AMP-binding (1526..1926): ser

NRPSPredictor2: ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic
Large clusters prediction:: ser, thr, dhpg, hpg
Small clusters prediction:: ser
Single AA prediction:: ser

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHlSLVDK
Nearest Stachelhaus code:: ser
Stachelhaus code match:: 90% (moderate)

F583_RS0124840

PKS_AT (551..747): mal

ATSignature: (unknown)

No matches above 50%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 67.4
Methoxymalonyl-CoA: 47.4
Methylmalonyl-CoA: 46.2
2-Methylbutyryl-CoA: 36.8
Propionyl-CoA: 34.8
Ethylmalonyl-CoA: 34.6
inactive: 34.4
trans-1,2-CPDA: 33.8
CHC-CoA: 31.6
Acetyl-CoA: 24.7
Isobutyryl-CoA: 22.0
fatty_acid: 21.5
Benzoyl-CoA: 19.3
3-Methylbutyryl-CoA: 10.4

F583_RS01000000127215

CAL_domain (41..485): pk

minowa: NH2

Prediction, score:

NH2: 175.7
Acetyl-CoA: 60.4
AHBA: 11.7
shikimic_acid: 0.0
fatty_acid: 0.0

PKS_AT (1208..1507): mmal

ATSignature: Malonyl-CoA, Methylmalonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; Methylmalonyl-CoA: 79.2%; Propionyl-CoA: 70.8%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 142.9
Methoxymalonyl-CoA: 113.0
Malonyl-CoA: 95.4
Ethylmalonyl-CoA: 94.5
Benzoyl-CoA: 90.9
Isobutyryl-CoA: 88.7
Propionyl-CoA: 75.8
trans-1,2-CPDA: 63.6
2-Methylbutyryl-CoA: 61.7
3-Methylbutyryl-CoA: 58.5
fatty_acid: 49.8
Acetyl-CoA: 46.2
CHC-CoA: 36.4
inactive: 32.5