BGC0001331: BE-43547A1 biosynthetic gene cluster from Salinispora arenicola CNR107
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Location: 10,670 - 45,645 nt. (total: 34,976 nt).
This entry is originally from NCBI GenBank NZ_KB900339.1, but has been modified (see Modifications tab for details).

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
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Gene details
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General
Compounds
Genes
Polyketide
NRP
History
NRPS/PKS domains
KnownClusterBlast
Modifications
General information about the BGC
MIBiG accession BGC0001331
Short description BE-43547A1 biosynthetic gene cluster from Salinispora arenicola CNR107
Status Minimal annotation: no
A minimal annotation only contains information on the BGC loci and one or more linked chemical product(s)

Completeness: complete
Whether the loci encodes everything needed for the pathway producing the compound(s)
Remarks "The only prior publication on this class of molecules are a japanese patent from 1998. I put in a placeholder PMID until our paper is accepted."
Biosynthetic class(es)
  • NRP (Cyclic depsipeptide)
  • Polyketide (Macrolide)
Loci NCBI GenBank: NZ_KB900339.1
Compounds
  • BE-43547A1
  • BE-43547A2
  • BE-43547B1
  • BE-43547B2
  • BE-43547B3
  • BE-43547C1
  • BE-43547C2
Species Salinispora arenicola CNR107 [taxonomy]
References
Chemical products information
BE-43547A1
Copy SMILES
C30H49N3O7
BE-43547A2
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C30H49N3O7
BE-43547B1
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C31H51N3O7
BE-43547B2
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C31H51N3O7
BE-43547B3
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C31H51N3O7
BE-43547C1
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C32H53N3O7
BE-43547C2
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C32H53N3O7
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Comment Extra
  • F583_RS0124805
  • WP_019032749.1
 10670 - 12229 (-) hypothetical protein
  • Transport
Sequence-based prediction
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  • F583_RS0124810
  • WP_019032750.1
 12226 - 13152 (-) multidrug ABC transporter ATP-binding protein
  • Transport
Sequence-based prediction
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  • F583_RS01000000127200
  • WP_080648942.1
 13243 - 13821 (+) TetR/AcrR family transcriptional regulator
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  • F583_RS0124820
  • WP_019032752.1
 13879 - 14628 (-) hypothetical protein
  • Scaffold biosynthesis
Sequence-based prediction
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  • F583_RS01000000127205
  • WP_019032753.1
 14625 - 20300 (-) non-ribosomal peptide synthetase
  • Scaffold biosynthesis
Sequence-based prediction
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  • F583_RS0124830
  • WP_019032754.1
 20297 - 25753 (-) type I polyketide synthase
  • Scaffold biosynthesis
Sequence-based prediction
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  • F583_RS01000000127210
  • WP_019032755.1
 25750 - 32184 (-) non-ribosomal peptide synthetase
  • Scaffold biosynthesis
Sequence-based prediction
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  • F583_RS0124840
  • WP_019032756.1
 32181 - 35168 (-) type I polyketide synthase
  • Scaffold biosynthesis
Sequence-based prediction
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  • F583_RS01000000127215
  • WP_019032757.1
 35170 - 41919 (-) type I polyketide synthase
  • Scaffold biosynthesis
Sequence-based prediction
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  • F583_RS0124850
  • WP_019032758.1
 41961 - 42437 (-) hypothetical protein
  • Tailoring (Monooxygenation)
Sequence-based prediction
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  • F583_RS01000000127220
  • WP_019032759.1
 42434 - 42922 (-) hypothetical protein
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  • F583_RS01000000127225
  • WP_155247000.1
 43533 - 43769 (+) hypothetical protein
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  • F583_RS0124865
 43873 - >44205 (-) MMPL family transporter
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  • F583_RS1000000129165
  • WP_019032762.1
 44177 - 44347 (+) hypothetical protein
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  • F583_RS0124875
 44342 - 44587 (-) hypothetical protein
Derived by automated computational analysis using gene prediction method: GeneMarkS+.
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  • F583_RS0124880
  • WP_019032764.1
 44626 - 45645 (+) NAD(P)-binding domain-containing protein
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Polyketide-specific information
Subclass Macrolide
Cyclic? yes
Release type
  • Macrolactonization
Polyketide-synthases
Genes Properties Modules
F583_RS0124840
+
F583_RS0124830
+
F583_RS01000000127215 		Synthase subclass: Modular type I
Module 0
Genes: F583_RS01000000127215
Core domains: CoA-ligase, Thiolation (ACP/PCP)
Module 1
Specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
Genes: F583_RS01000000127215
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 2
Specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
Genes: F583_RS0124840
Core domains: Ketosynthase, Acyltransferase, Thiolation (ACP/PCP)
Module 5
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: F583_RS0124830
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: Unknown
NRP-specific information
Subclass Cyclic depsipeptide
Cyclic? yes
Release type
  • Macrolactonization
Thioesterase genes
  • F583_RS0124820 (Unknown)
NRP-synthases
Gene Modules
F583_RS01000000127210
Module 3
Specificity: glycine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
Module 4
Specificity: serine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
F583_RS01000000127205
Module 6
Specificity: glycine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
Modification domains: Methylation
Module 7 [inactive]
Condensation domain type: LCL
Non-canonical activity:
  • Other
Evidence for non-canonical activity:
  • Structure-based inference
Annotation changelog
MIBiG version Submitter Notes
1.4
  • Hidden contributor (ID: DPAKSDGGIIU3JRGIDNMS7RSG, no GDPR consent given).
  • Submitted
2.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Migrated from v1.4
  • Updated reference publication(s) (MIBiG Annotathon)
3.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Corrected NRP module activity
  • Removed gene names of 'No gene ID'
  • Removed ketoreductase stereochemistry annotation from modules without ketoreductases
  • Removed extra gene with no information
  • Changed amino acid substrates to lower case
3.1
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Update chemical activity to schema version 2.11
Detailed domain annotation
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Modifications to original record
  • F583_RS0124875 was added