LOCUS       BGC0001336             12620 bp    DNA     linear   ENV 29-DEC-2013
DEFINITION  Uncultured bacterium clone AB339 erdasporine tryptophan dimer gene
            cluster, partial sequence.
ACCESSION   BGC0001336
VERSION     BGC0001336
KEYWORDS    .
SOURCE      uncultured bacterium
  ORGANISM  uncultured bacterium
            Bacteria; environmental samples.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-08 00:46:51
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 12620
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..1059
                     /codon_start=1
                     /gene="espM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1042:O-methyltransferase (Score: 324.4; E-value:
                     1.4e-98)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="asmbl_3"
                     /note="EspM; [QR] COG0500 SAM-dependent methyltransferases"
                     /product="O-methyltransferase"
                     /protein_id="AHE14647.1"
                     /transl_table=11
                     /translation="MTWSPGMNHGDQQKDHAFRLLQMSEGFGVARAVQLAAELGVADLL
                     GDAPRSADDLAEATSTHPGALYRLLRALAGVGLFTEAEPGRFGLTPAGEQLRADHPESL
                     RSWVMFQGLFNGVYAESMHSLRTGEPTVPLVFGEPLFAHLQHHPEHGRIFNAAMGQHSR
                     LTGKALAAAYDFTGARQIVDVGGGDGSFLSAVLRAHSESSGVVFDQPYLSDAAGKQLGA
                     AGLGERCRFVGGDFLRDVPSGGDVYLLKGVLHNWPDDQAVVMLRNCRRAMGADGRLLLI
                     EWVVPDGDVPHPSKFLDLAMLFVYGGRERTEKEYVALLTEAGLRINRILGSRSTLDVLE
                     VVPAEKKRDSAR"
     protocluster    1..12620
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[4281:7596]"
                     /cutoff="20000"
                     /detection_rule="(indsynth or dmat or indole_PTase)"
                     /neighbourhood="10000"
                     /product="indole"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      4282..7596
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(indsynth or dmat or indole_PTase)"
                     /neighbourhood="10000"
                     /product="indole"
                     /protocluster_number="1"
     cand_cluster    1..12620
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(indsynth or dmat or indole_PTase)"
                     /kind="single"
                     /product="indole"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..12620
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0001336"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..12620
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="indole"
                     /region_number="1"
                     /rules="(indsynth or dmat or indole_PTase)"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            1..1059
                     /gene="espM"
                     /locus_tag="asmbl_3"
     gene            1144..2796
                     /gene="espX"
                     /locus_tag="asmbl_4"
     CDS             1144..2796
                     /codon_start=1
                     /gene="espX"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1050:monooxygenase FAD-binding (Score: 355.2; E-value:
                     1.1e-107)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="asmbl_4"
                     /note="EspX; [HC] COG0654 2-polyprenyl-6-methoxyphenol
                     hydroxylase and related FAD-dependent oxidoreductases"
                     /product="FAD-binding monooxygenase"
                     /protein_id="AHE14649.1"
                     /transl_table=11
                     /translation="MVHDVDVLVVGAGPVGLTLAIALRRLSLRVRVVDKASGTNREPRA
                     DVIFPRAGEALGAIGVGETIRDRSYQMQGANFYADGRHIGSCKTGRLASEYPRAMTIEQ
                     HEIERLLAAELAELGVDVEWRTEVKDVLAFPDRVHVPMVRPDGTTEVADAAWVVACDGI
                     RSTVRSLVGIPFEGVRRHNMQVVQGNVVPSWPLSDRPGHGYFFLAPRRTVIAFPTPSGG
                     YRVFCVRDDPQPERRGAPTLGELRDLVAEVAHIPDLQFTLTDPVWLSRSRFADRVAARL
                     RHGRVLLAGDAAHAWAPIGGHGMNVGMLGAHNLAWKIAAVHKGQTPDALLDSYDVEQRA
                     LAHGVIRDMRRNILEVLLPPVAHRARSALLRVTLSQDAVQRRTEWMMSDFGRNHRRSPL
                     SWQPRGWRVRRLRAGDRMPDVTVVPSAVVGDRRQDPSASNGQPVRLHRLLRYDRWTLLV
                     DAVRTDAETLRAIEHACAAAPVEIVAITTTDPEARRQLGRASELKLVRPDGYVGLVARL
                     DRPGTLRDYIDTFLSGVRDSSTEKDQGAFSEVG"
     gene            2783..4282
                     /gene="espO"
                     /locus_tag="asmbl_5"
     CDS             2783..4282
                     /codon_start=1
                     /gene="espO"
                     /locus_tag="asmbl_5"
                     /note="EspO; [E] COG1231 monoamine oxidase"
                     /product="indole-3-pyruvic acid imine synthase EspO"
                     /protein_id="AHE14640.1"
                     /transl_table=11
                     /translation="MRWDEALPNTGPAGKSTVTVIGAGIAGLVAAYELEQLGYQVEVLE
                     GSRRIGGRIYTHRFGFEAEAPVAELGAMRIPTKHRHTMEYISRLGLAGELRPFKSLLAD
                     ENAFLKTSTGFVRLRNAPQPLLEDLRQGLSHNGYREETLLFGAGLSLIVNAIAPPAVRE
                     GLRQALHAELLDLADRLDLRPHLRENGTRQMDVHAVFAAHPHLQAACKGDLGSFLDDIL
                     TETDSELVRVRGGMSRIVQRLARRIQGRILRGREVLSLDVRPDGVVMGIREGGRIVSRH
                     SRYVVCTVPFPVLRRMRLTGLSQDKLDAIRQVQYVSATKVALHCREPFWEGAGITAGAS
                     CSGGRIRQTYYPCADGDPALGAVLLASYTIGDDADLLGRLPSAARYASIIAELAEMHPE
                     LLRPGMVLNAASIAWGQHRWSGGGCSVRWGKDAAACEEERIRAARPQHTLFFAGEHCSS
                     TPAWIDGAIESAVVAVRQLVLHDAEPGTAKASELAASAEVS"
     gene            4282..7596
                     /gene="espD"
                     /locus_tag="asmbl_6"
     CDS             4282..7596
                     /codon_start=1
                     /gene="espD"
                     /gene_functions="biosynthetic (rule-based-clusters) indole:
                     indsynth"
                     /gene_kind="biosynthetic"
                     /locus_tag="asmbl_6"
                     /note="EspD"
                     /product="chromopyrrolic acid synthase"
                     /protein_id="AHE14639.1"
                     /sec_met_domain="indsynth (E-value: 0.0, bitscore: 1520.5,
                     seeds: 14, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSVFDLPRLHFGGTAVTRLPTGPRSGLLDLATNRALTQHGPFPVD
                     RPVEEYHEYLDRHGLRFDSEGRTDPGGVFGATKGTNFGGNGHFWIDAAIVSAEGPDGLQ
                     ADPVVGRAVDMWGHYNEYLATTVNRGRVFDVDPTSNWTTTLMVGQFGFGRSGRSHDTGY
                     MVTGNVSGMHPPRWHRPDYVRDVGGHYLAPQMRRSVVYQFVVSKDEGLDWLPESSASPI
                     ATLLRSVVSSADMQGLVVQFALSNMAPPITNDAPDRWDVRGTIAPWRGQELRTYPAGRL
                     LTPRAAGRGSGQTLHNLTASIEDDRVTLNMITAVPVTGRTPGSGTGPTPALGPRCDLGD
                     LELRAAHTGRLVARIPVAAYRGGTYDLTSGIVTALSELPGEIVDDEPLCVTGAVPGGER
                     AVLLQEEEVNLQVDDACLFLEHPNRPSGDDHPAEVMVRSFVRGRPGPVDTVHVRPFVNP
                     RSRPLDPAMAGARCGDVDIVEVREGRLDGSGGYGPTCVTSTDSQGRGWFTMRGVRAGAG
                     RILISGREDDLPCDPDAAESAARAYDDADQLGYWAGAGWMTVRVLPDDWHLDAVASDDV
                     TFELVYREVFAYYELLYSFMKAEVFSLADECKVKTYPRLIWQMCDPRNRSKTYYMPPTR
                     DMSEPKARLLLKFLRAQQSVTDIPIVVPVPRTDTRGITTRGELLAALKWAATIEVAVML
                     QYLYAAYSIPTYGGGREHVRRGLWTPEQLRLACGDGGETTDGGVRGALLSVAREEMIHF
                     LMVNNIIMAIGEPFHIPVVDFGTINSTLPVPLDFALEPLGVGSLQRFIAIERPEAQSGE
                     IRRAAGQGFSSYDSSPFAGSAGGPVRSYSSLSELYEDIREGLQRVPDLFMVSKGRGGGE
                     HHLFLRESINTVHPDYQLEVDDLPSALFAIDVVTEQGEGNKLTSVTPSGESHYETFLRL
                     SDLLMAEQMAGPRAPRPPWTPAYPVPRNPTVLEGNANRELVTDPDARAVMHLFNRSYYL
                     MFQLMVQHFGQTPDASLRRSKLMNAAIDVMTGMMSPLAELLVTMPSGRRGKTAGPSFDL
                     EDEPRYNSRPDVAMRSVALRFSHLAAAARKCPAVPARVTDMYSFYAEYFRHFQQAP"
     gene            7628..9034
                     /gene="espP"
                     /locus_tag="asmbl_7"
     CDS             7628..9034
                     /codon_start=1
                     /gene="espP"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007:cytochrome P450 (Score: 383.2; E-value:
                     2.3e-116)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="asmbl_7"
                     /note="EspP; [Q] COG2124 cytochrome P450"
                     /product="cytochrome P450"
                     /protein_id="AHE14641.1"
                     /sec_met_domain="p450 (E-value: 1.1e-26, bitscore: 82.4,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTQRGTGQFALTAEDIADPYPVYRHYREVDPVHVVPAASPDLPST
                     WYLFRYADVVRVLSSPHVGRSAQRACAHLADPPALIPAHYPVLREVVSGWLVFLDPPRH
                     TRLRALVATQFSPRVVTGLRVRVDRYAHELLLSMREKEHTDLVEDYSAPLPILVISELL
                     GIPRERRAWMRDRAVALQEANSSRTDGRPDRYATAEAAARDLRDYFLDEIRLRRRREYD
                     DLTALLVRDHDGDGSLTDAEIVSTCIHLLTAGHETTTNLVSKATLALLAHPGTRDELRT
                     DPQLMPGAVDELIRYDPPVQMITRWAYRDLVIAGRTISRGSRIVLVLGSANRDPERFAE
                     PDTLDIRRNASRHCGFGMGIHYCLGAPLARLEAEVALVALLRELPHLATTGETVRYADD
                     LVFHGPSRLLLRTGKPPGPNGTPDGHVPASGPSVPANRADRPFRGVTASAAAAPSIEPD
                     VERHGRVDPL"
     gene            9259..10971
                     /gene="espT1"
                     /locus_tag="asmbl_8"
     CDS             9259..10971
                     /codon_start=1
                     /gene="espT1"
                     /gene_functions="transport (smcogs) SMCOG1005:Drug
                     resistance transporter, EmrB/QacA (Score: 377.7; E-value:
                     1e-114)"
                     /gene_kind="transport"
                     /locus_tag="asmbl_8"
                     /note="EspT1; [GEPR] COG0477 permeases of the major
                     facilitator superfamily"
                     /product="putative transporter EspT1"
                     /protein_id="AHE14650.1"
                     /transl_table=11
                     /translation="MLAVVLSATFMQLLDASIVSIAVPPLQADLGTTYSELQLVVGGYL
                     LTFACALLVGGRLGDTYGRRNLFLIGMAGFTAASALCGLAPDPTTLVIARLVQGLFAGL
                     MFPQVLSIIQVTFPPDQRTRVHGIYGATLGVASILGPLLGGLFVEADLFGTGWRMIFLV
                     NIPIGLAALAVGVVVLSESKARDAGRVDLPGAASVGAGLFLVVLPLTIGGHMGWPVWTY
                     MMLVGGLLSLAVFVRRQRRRSLWQHYVRRADAPLIPWALFWYRSFTVGLVLNLVFFSGV
                     APFFFYFIIYLETGAGFSPLRAGLTVLPVAVASAVASPAAAAIGRRHGRLGLAVGCALL
                     ALGMGGLVMTLRHTGDQPSLWLFVPALLLVGLGLGVFGSLVTSVVLDSIGDNEAGAASG
                     ALATAQQVGGAIGVAIVGVLFFGLIGINADRASSSQVTELKGALAAADLPPATIQSTVD
                     GFRTCFRERAHQKDVSATPAACLRGRQEVDRALVGASPQVKELVGTALEAAGKRSVTRD
                     FTRSTAQTTLYEIAVFALALLLVLALPRSQHSPTQARRGRGAPGRHRSGRRGA"
     gene            11265..12620
                     /gene="espT2"
                     /locus_tag="asmbl_9"
     CDS             11265..12620
                     /codon_start=1
                     /gene="espT2"
                     /gene_functions="transport (smcogs) SMCOG1202:major
                     facilitator transporter (Score: 81.7; E-value: 6.4e-25)"
                     /gene_kind="transport"
                     /locus_tag="asmbl_9"
                     /note="EspT2; [GEPR] COG0477 permeases of the major
                     facilitator superfamily"
                     /product="putative transporter EspT2"
                     /protein_id="AHE14642.1"
                     /transl_table=11
                     /translation="MEELLQQRTPDVDLASASGVEAQTRSGEPSQPVGLGFILPFALAY
                     FGFWMTLLTPPIVGMALYLSKVLPENQRNGALGLVLAMGGVIGVLVNPIVGKLSDRTTS
                     RYGMRKPWMVGGTIVAIVGLLVTATSTTMPLILLGYCIASFGLNATAATLIPLLPDQVP
                     HRQRGRVSGILGMGLPVGAVGGAGLAQAFAANPLWMFLAPAVVLAIGVAVLVTRYEDRT
                     LAAAQAARLPRYGVREFLASFWVSPKRHPAFAWTWLSRFLVFMGVATLVTFQSVYLIEH
                     LGFPLIAVPMLVALATSVHYLFVFAASPVAGWLSDRMDRRKIFVALAALFYAVGLGVIA
                     SAESFTAFLIGMAITGIGEGAYSAVDLALVTDVLPNPDDAGKEMGVFAVANTLPPAIAP
                     AIAPALLAIPFLGRGDGGNFVALFAVAALLAVLGAVAISPVRTARDRSNVAV"
     misc_feature    11277..11279
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
ORIGIN
        1 atgacttgga gcccaggaat gaatcacggt gaccagcaaa aggaccatgc gttccgcctg
       61 ctgcagatgt ccgagggatt tggcgtggcc cgggctgtgc agctcgcggc cgaactcggc
      121 gtggcggacc tactcggcga cgcgccccgg agcgccgatg atttggccga agccacctcg
      181 acccaccccg gggcgctgta ccggttgctt cgcgcattgg ccggtgtcgg cctgttcacg
      241 gaggcggagc cgggccggtt cgggttgacg ccggccggtg agcagctgcg cgcggaccac
      301 ccggagtcgc tccgatcgtg ggtgatgttt cagggtctgt tcaacggcgt gtacgcggag
      361 tcgatgcaca gcctgcggac gggagagccg acggttccac tggtcttcgg cgagccgctc
      421 ttcgcccacc tgcagcacca ccccgagcac ggcaggattt tcaacgcggc gatggggcag
      481 cacagtcggt tgaccggcaa ggctctggcc gccgcctacg acttcaccgg agcacggcag
      541 atcgtcgatg tcggtggcgg ggacggcagt ttcctgagcg cggtgctgcg ggcacattcc
      601 gagagttcgg gcgtggtgtt cgaccagccg tatctgagcg atgcggccgg taagcagctc
      661 ggcgccgccg ggctcggcga gcgctgcagg ttcgtcggtg gcgatttcct gcgggacgtt
      721 ccctccggcg gagacgtata cctgctcaag ggcgtcctgc acaactggcc cgacgatcag
      781 gccgtggtga tgctgcgcaa ctgccgccgc gcgatgggcg ccgacgggcg gctcctgctc
      841 atcgaatggg tggtgcccga cggcgacgta cctcatccga gcaaattcct ggatctcgcc
      901 atgttgttcg tctatggcgg acgggagcgg accgaaaagg agtacgtcgc gcttctgacc
      961 gaggcgggcc tgcgcatcaa ccggattttg ggttcgcgat cgacgctcga cgtgctcgag
     1021 gtcgttcccg ctgagaagaa aagggattcg gcgcgatgac cacaatggcg cccctgaggg
     1081 gcggacatcg aaatttgggt cgcaatggaa ttggtggatc aggaacgcct gtcggaggtt
     1141 ggcatggtgc atgacgttga cgtgctcgtg gtcggcgcgg gtccggtagg gctgaccctc
     1201 gcgatcgccc tccggcgcct gtcgttgcgg gtgcgggtgg tcgacaaagc gtcgggtacg
     1261 aatcgtgaac ctcgggcaga tgtgatcttc ccacgggcgg gcgaagccct cggggccatc
     1321 ggcgtgggcg agacgatccg ggaccgttcg taccaaatgc agggagcgaa cttctacgcg
     1381 gacggccgcc acatcggctc gtgcaagacc ggacggctcg cctcggagta cccgcgcgcc
     1441 atgacgatcg agcaacacga gatcgagcgg ctgctcgccg cggagctggc cgagctgggc
     1501 gtcgacgtgg agtggcggac cgaggtcaaa gacgtgctgg cgttcccgga ccgggtccac
     1561 gtgcccatgg tccgcccgga cggcaccacc gaggtcgccg acgccgcatg ggtggtcgca
     1621 tgcgacggca tccgcagcac ggtccgcagc ttggtcggta ttccgttcga aggcgtacgt
     1681 cggcacaaca tgcaggtggt ccagggcaac gtcgtcccgt cttggccact gagcgaccga
     1741 ccgggccacg gttacttctt cctggccccg cggcggacgg tcatcgcctt ccccacgcct
     1801 tccggcgggt accgcgtctt ctgtgtccgg gacgaccccc agccggaacg caggggggcg
     1861 ccgacgctcg gcgaattgcg cgacctggtc gccgaggtcg cgcacatccc ggacctccag
     1921 ttcaccctca ccgacccggt gtggctgagc cggagccggt tcgccgaccg ggtcgccgcc
     1981 cggctgcggc atgggcgggt gctgcttgcc ggggacgcgg cccacgcctg ggcgcccatc
     2041 ggtgggcacg ggatgaacgt gggcatgctc ggcgcgcaca acctggcctg gaaaatcgcc
     2101 gccgtccaca agggccagac acccgatgcc ctgctcgact cctacgacgt cgagcagcgc
     2161 gcgctcgccc acggcgtcat ccgcgacatg cgccggaaca tcctcgaggt actcctgccg
     2221 cccgtcgcgc accgggcgcg ctccgccctg ctcagagtca ccctgtccca ggatgccgtc
     2281 cagcggcgga ccgagtggat gatgagcgac tttggccgca accaccggcg gagtccgctg
     2341 tcgtggcagc cacgagggtg gcgcgtgcgc cggctgcgtg ccggtgatcg gatgccggac
     2401 gtcaccgtgg ttcccagcgc cgtcgttggc gaccggcggc aggacccgtc cgcgtcgaac
     2461 gggcagccgg tgcgcctgca ccgcctcctc aggtacgacc ggtggacatt gctcgtcgac
     2521 gccgtccgca ccgacgcgga gaccctgcgc gccatcgagc atgcctgcgc cgccgcgccg
     2581 gtggagatcg tcgccatcac cacgaccgat cccgaggcac gccgccagct gggcagggcg
     2641 agcgagctca agctcgtacg gcccgacggc tacgtcggcc tcgtcgccag gctcgaccgc
     2701 ccggggacgc tgcgcgacta catcgacaca ttcctttccg gagtccgcga cagctcgacc
     2761 gagaaagatc agggggcctt cagtgaggtg ggatgaggcg ctaccgaaca ccgggccggc
     2821 cgggaagagc acagtcacgg tcatcggtgc gggcatcgcc ggcctggtgg ccgcgtacga
     2881 gctggagcag ctcggctacc aggtggaggt cctggagggg agccgccgga tcggcgggcg
     2941 catctacacg caccgcttcg gcttcgaggc ggaagcgccc gtcgccgagc tgggcgcgat
     3001 gcgcatcccc accaagcatc gccacacgat ggagtacatc tcccggctgg ggctggccgg
     3061 cgaactgcgg ccgttcaaga gcctgctggc cgacgagaac gccttcctca agaccagcac
     3121 cggcttcgtc cgtctccgca acgccccgca gccgttgctc gaggaccttc gtcagggtct
     3181 ttcccacaac ggctaccggg aggagacgct gctgttcggc gccgggctga gcctcatcgt
     3241 caacgcgatc gccccgcccg ccgtgcgtga agggctgcgc caagccctcc acgcggaact
     3301 gctggacctg gccgaccggc tggacctgcg accccacctg cgcgagaacg gcaccaggca
     3361 gatggacgtg cacgcggtat tcgccgcaca tccgcatctg caggcggcct gcaaagggga
     3421 tctcggaagc ttccttgacg acatcctgac cgagacggac tctgagctgg tacgggtgcg
     3481 gggcggcatg agccggatcg tgcagcggct ggcacgccgg atccaggggc ggatcctccg
     3541 cggcagggaa gtcctgagtc tggatgtccg gccggacggc gtggtgatgg ggatccgcga
     3601 aggcggccgg atcgtcagtc gccacagccg gtacgtggtg tgcacggtgc cgtttccggt
     3661 gctgcgcagg atgcggctca cgggtctcag ccaggacaag ctggatgcga tccgtcaggt
     3721 gcagtacgtg tccgcgacga aggtggcgtt gcattgccgg gagccgttct gggagggcgc
     3781 gggcatcacc gccggcgcct cgtgcagcgg tgggcggatc cggcagacct actacccgtg
     3841 cgcggacggg gatccggcac tcggggcggt gctgctggcc agttacacca tcggggacga
     3901 cgcggacctg ctgggccggc tgccgtcagc cgcacggtac gcgtccatca tcgcggagct
     3961 ggccgagatg cacccggagc tgctgcgtcc cggcatggtg ctcaacgcgg ccagcatcgc
     4021 gtggggccag catcggtggt ccggcggtgg ctgctcggtg cgttggggca aggacgccgc
     4081 cgcgtgtgaa gaggagcgga tccgcgcggc ccgcccgcag cacacactgt tcttcgcagg
     4141 ggagcactgc tcgtcgacac cggcctggat cgacggagcc atcgagtcag ccgtcgtcgc
     4201 cgtgcggcag ctggtgcttc atgacgcgga accgggtacg gctaaagcca gcgagctggc
     4261 cgccagcgcg gaggtgtcgt gatgagtgtt tttgatctgc cccgtctgca tttcggtggg
     4321 accgcggtca cccggcttcc caccggtccc cgcagtggtc tgctggacct ggccacgaat
     4381 cgggcgctca cccagcacgg gccgtttccg gtcgacaggc cggtcgagga gtaccacgag
     4441 tacctggatc ggcatggcct ccggttcgac agcgagggcc ggacagatcc cggaggggtc
     4501 ttcggcgcga ccaagggcac caatttcggt gggaacggcc atttctggat cgatgccgcg
     4561 atcgtcagcg ccgagggtcc cgacggcttg caggccgatc ccgtggtggg ccgggcagtc
     4621 gacatgtggg gccactacaa cgaatacctg gcgaccacgg tgaaccgggg ccgagtcttc
     4681 gatgtggacc cgacgtcgaa ctggacgacc acgctcatgg tcgggcagtt cggcttcggc
     4741 cgatccggcc gctcgcacga cacggggtac atggtcaccg gaaacgtgtc cggcatgcac
     4801 ccgccccggt ggcaccggcc ggactacgtc cgggacgtgg gcggccacta ccttgcgccg
     4861 cagatgcgcc gatcggtggt ctaccagttc gtggtgtcca aggacgaggg cctcgactgg
     4921 ctcccggaat cgtcggcctc cccgatcgcc acgctgctgc gctcggtggt gagctccgcc
     4981 gacatgcagg gactggtcgt gcagttcgcg ttgagcaaca tggccccgcc gatcaccaac
     5041 gatgcgcctg accggtggga cgtgcggggg acgatcgccc cgtggcgcgg gcaggagctg
     5101 cgcacctacc ccgccgggcg cctgctcacc ccacgcgcgg ccggtcgggg atccggtcag
     5161 accctgcaca acctgacggc ctccatcgag gacgaccggg tcaccctgaa catgatcacg
     5221 gctgttcccg tgaccggtcg cacccccggt agcggcaccg gtccgacgcc cgcgttgggg
     5281 ccacggtgcg acctcgggga cctggaactg cgtgcggcgc acacgggcag gctcgtggcc
     5341 agaatccccg tcgcggcgta ccggggtgga acctatgacc tgacgagcgg catcgtgacc
     5401 gctctctccg agctccccgg cgagatcgtc gacgacgagc cgctctgtgt gaccggggcc
     5461 gttcccggcg gcgagcgggc tgtgctgttg caggaagagg aggtgaacct ccaggtcgat
     5521 gacgcgtgcc tcttcctcga gcacccgaac aggccgagcg gcgacgacca cccggccgag
     5581 gtcatggtcc gctccttcgt ccggggccgg cccggaccgg tcgacaccgt gcacgtgcgg
     5641 ccgttcgtca atccccggtc ccgtcccctg gaccccgcca tggcgggagc acgctgtgga
     5701 gacgtcgaca tcgtcgaggt gcgcgagggc cggctggacg gatccggcgg gtacggaccc
     5761 acctgcgtca cgagcaccga ctcgcagggc cgtgggtggt tcaccatgcg gggcgtgcgg
     5821 gccggcgccg gccggattct gatcagcggg cgcgaggacg acctgccgtg cgatccggat
     5881 gcggcggaat ccgcggctag ggcgtacgac gacgcggacc agctcggcta ctgggccggc
     5941 gcgggctgga tgaccgtgcg ggtcctgccg gacgactggc atctggacgc cgtggcgagc
     6001 gacgacgtca ccttcgagct cgtgtaccgc gaggtgttcg cctactacga actgctctac
     6061 tcgttcatga aagcggaggt cttcagcctg gccgacgagt gcaaggtcaa gacctatccc
     6121 cggctgatct ggcagatgtg cgatccgcgc aacaggtcga agacctacta catgccgccc
     6181 acccgcgaca tgtccgagcc caaggcgcga ctgctgctga agttcctgcg cgcccagcag
     6241 agcgtcaccg acatcccgat cgtggtaccg gtacccagaa cggacaccag aggaatcacc
     6301 acgcgcgggg agctgcttgc agcgctgaaa tgggcggcga cgatcgaggt ggcggtgatg
     6361 ctgcagtatc tctacgccgc gtactcgatc cccacctacg gcggcggccg ggagcatgtg
     6421 cggcgcggcc tgtggacgcc ggagcagctg cgcttggcgt gcggcgacgg tggggagacg
     6481 acggatggtg gggtgagggg tgccctgctc tccgtggcac gcgaagagat gatccatttc
     6541 ctgatggtca acaacatcat catggcgatc ggtgagccgt tccacattcc ggtggtggac
     6601 ttcggcacga tcaacagcac cttgccggtt cccctcgact ttgccctgga gcccctcggt
     6661 gtcggcagtc tccagcggtt catcgccatt gaacggccgg aggcccagtc cggtgagata
     6721 cggcgtgcgg caggccaggg cttctcgtcg tatgactctt cgcccttcgc ggggtcggcc
     6781 ggcggaccgg tgcgttcgta cagctcgctc agtgagctgt acgaggacat tcgcgagggc
     6841 ctgcagcgcg tacccgacct gttcatggtc tccaagggcc gcggcggcgg ggagcaccac
     6901 ctgttccttc gggagtcgat caacaccgtc caccccgact accagctcga ggtcgacgac
     6961 ctgcccagcg cgctgttcgc catcgacgtc gtcaccgagc agggcgaggg aaacaagctc
     7021 acctcggtga ccccgtccgg agaatcacac tacgagacgt tccttcgcct gtccgacctg
     7081 ctgatggccg agcagatggc gggccctagg gcgccccgcc cgccctggac gccggcctac
     7141 ccggtgccgc ggaaccctac ggtcctggag ggcaacgcga atcgggaact ggtcaccgac
     7201 ccggacgccc gagccgtcat gcacctgttc aaccggtcct actacctgat gttccagctg
     7261 atggtgcaac acttcgggca gacgccggac gccagcctgc ggcgctccaa gctgatgaac
     7321 gcggccatcg acgtcatgac cgggatgatg agcccgctgg ccgaactact ggtgacgatg
     7381 ccgtcgggtc ggcgcggaaa gaccgctggg ccgtccttcg atctcgagga cgagccgcgg
     7441 tacaactctc ggccggatgt cgccatgcgc tcggtcgcgc tgcggttctc ccacctggcc
     7501 gcggccgccc gcaagtgccc ggcggtaccc gcccgcgtca cggatatgta ctccttctac
     7561 gccgagtact tccgccactt ccagcaggcg ccgtaatggc ccttccctgc gtgcccgagg
     7621 acgaacaatg acgcagcgcg gtacaggaca gtttgctctc accgccgagg acatcgccga
     7681 cccctacccg gtctaccggc actaccgcga ggtcgatccg gttcatgtcg ttccggcggc
     7741 ctcgccggac ctgccgtcca cctggtacct gttccggtac gcggatgtgg tgcgtgtcct
     7801 gtccagcccg cacgtcgggc gcagcgctca gcgtgcctgc gcccacttgg cggacccgcc
     7861 ggcgctgatc ccggcccact acccggttct gcgggaggtc gtgagcggct ggctggtctt
     7921 cctggatccg ccccgccaca ccaggctgcg cgcgctcgtg gccacgcagt tctcgccccg
     7981 tgtggtgacc ggcctgcgtg tccgggtcga cagatacgct cacgaactgc tcctgtccat
     8041 gcgagagaag gaacacaccg acctggtcga ggactattcc gctcccctgc ccatcctggt
     8101 gatctccgaa ctgctcggca taccgcggga gcgtcgcgcg tggatgcgcg atcgtgcggt
     8161 agcacttcag gaggcgaaca gctcgcgcac cgacgggcgc cccgatcggt acgccacagc
     8221 cgaggcggcc gcccgggacc tgcgcgacta cttcctcgac gagatccggc tacgccggcg
     8281 gcgggagtac gacgatctga cggccctgct ggtacgagac cacgatgggg atggatcgct
     8341 gaccgacgcg gaaatcgtct ccacctgcat ccatctgctc accgccggcc acgagaccac
     8401 caccaacctc gtctccaaag ccacgctcgc ccttcttgcc cacccgggca cacgggatga
     8461 gcttcggacc gacccgcagc tcatgcccgg cgcggtggac gaactcatcc gctacgaccc
     8521 gcccgtacag atgatcaccc ggtgggcgta ccgggacctg gtgatagccg gccgaacgat
     8581 ctcccgcggc agcaggatcg tcctcgtcct cggctccgcc aatcgcgacc ccgagcggtt
     8641 cgcggaaccc gacaccctgg acatccgccg caatgcgagc cggcactgcg ggttcggtat
     8701 gggaatccac tactgtctcg gtgccccact ggcacggctg gaggccgaag tcgccctcgt
     8761 cgctctcctg cgagaactgc cgcacctcgc caccaccggg gaaacggtgc gctacgccga
     8821 cgatctcgtc ttccacggcc ccagccggct actgctgcgc acgggcaagc cgccaggccc
     8881 aaacggaact cccgatggac atgtgccggc gtccggcccg tccgtgcccg cgaaccgcgc
     8941 cgaccggcca ttccggggcg tgaccgcttc ggcggccgcg gctccgtcca tcgaaccgga
     9001 cgtcgaacgc catgggaggg tcgatccgct gtgaacgccc agtgcacgga gtgcaaggca
     9061 tgggcccgtg gcaacgggga gatgaccgag aatctcccgg tcaccgatgt catcgggccg
     9121 gcgcgggccg gacaggatcg gaagccgccc aggcccccgg ggctggcccg gccggccgtg
     9181 ccggaaccga ggacgggaac gccccagcca caattccagc cagcccggcc ggagcggccg
     9241 aaggggaggc actttctggt gctggcggtc gtgttgtccg cgaccttcat gcaactactc
     9301 gacgcgagca tcgtgagcat tgccgtccca ccgcttcagg ccgacctggg taccacctac
     9361 tcggaactcc agttggtggt cggcggctac ctgctgacct tcgcctgtgc cctgctcgtc
     9421 ggcggacgcc tcggcgacac gtacggccgc agaaacctct ttctgatcgg catggccggc
     9481 ttcaccgccg cgtccgcgct ctgcggactg gcgccggacc ccaccaccct ggtcatcgcg
     9541 cgcctcgtcc agggcctgtt cgccggattg atgttcccgc aggtgctctc gatcattcag
     9601 gtcaccttcc cgcccgacca gcggacccgg gtgcacggga tctacggagc gacgctcggc
     9661 gtcgcctcca tcctcggccc gctgctcggc ggcctcttcg tcgaggccga tctgttcggc
     9721 accggctggc gcatgatctt cctggtgaac atcccgatcg ggctcgcggc gctcgccgtc
     9781 ggagtagtgg tcctttccga gtcgaaagcg cgggatgcgg gccgagtcga cctgcccggc
     9841 gcggcatcag tcggggccgg ccttttcctc gtggtcctgc cgttgaccat cggcggccac
     9901 atgggctggc ccgtatggac ctacatgatg ctggtcgggg ggctactctc cttggccgtc
     9961 ttcgtcagac ggcagcgccg ccgctcgctg tggcagcact acgtccggcg cgccgacgcg
    10021 ccgctgatcc cgtgggcgct gttctggtat cgctcgttca ccgtcggact cgtgctgaac
    10081 ctggtgttct tctctggggt cgcgcccttc ttcttctact tcatcattta cctggagacg
    10141 ggggccggct tctccccgct gcgcgctgga ctcaccgtcc ttccggtcgc tgtcgcttcc
    10201 gcggtggcgt cgcctgccgc ggcggccatc ggccggcgtc acggccggct cgggttggcc
    10261 gtcggttgcg cgctgctggc cctcgggatg ggcgggctcg tcatgacgct gcgacacaca
    10321 ggtgaccagc cctccctgtg gttgttcgtt cccgcactcc tgctggtggg gctggggctc
    10381 ggcgtgttcg gctcgctggt cacctcggtg gtcctcgaca gcatcggcga caacgaggcg
    10441 ggcgccgcct ccggagcgct ggcgaccgct cagcaggtcg gcggagccat cggcgtcgcg
    10501 atcgtcggcg tcctgttctt cggcttgatc ggtatcaatg ccgatcgcgc gagcagtagc
    10561 caagttactg agctgaaggg cgcattggcg gcagcggacc tgccgccggc gaccatccag
    10621 agcaccgtcg atgggtttcg cacctgtttc cgcgaacgcg cccaccagaa ggacgtttcg
    10681 gccaccccgg ccgcctgtct acggggccgc caagaggtgg accgagcact cgtcggcgcg
    10741 tcgccgcagg taaaggagct ggtgggcacc gccctggaag cggcgggcaa gcgcagcgta
    10801 acgcgcgact tcacgcgctc gaccgcgcag accactctgt acgagatcgc agtcttcgcg
    10861 cttgctctcc tgctcgttct cgctctgccc cgatcccagc actccccgac ccaggcccgg
    10921 cggggccgtg gtgcgcccgg ccgacaccgg tccggccggc ggggcgcctg agcgcgacca
    10981 tctccggccc agaccttcag cgcgccaagg gtgtagcgct gtgcatttcc gcagatcctg
    11041 gagagctctt tccacaggca gggtggtcgg ttcacccgcg gcctcgtgat gccggagcaa
    11101 aacgggtgtg gccgagcaac cggcaaacac atattcatcc gggtctgtga cgcgccctac
    11161 atgactcgta attcgaatca ctgacagcaa cctgatagta agcgaagaat cgcgcgcgag
    11221 ggccgtacct tattgattac caccattagt ggaggaggaa ttcaatggag gagttgttac
    11281 agcagagaac gcccgacgtg gatctggcgt cggcgagtgg ggtcgaagca cagacacgtt
    11341 ccggggaacc gtcgcagcca gtggggctcg gtttcatcct gccgttcgcc ctggcgtact
    11401 tcgggttctg gatgactctg ctgacgcccc cgatcgtggg gatggcgttg tacctgtcca
    11461 aagtcctgcc ggagaaccag cgaaacgggg ccctggggct cgttctcgct atgggcggcg
    11521 tgatcggggt gctggtcaac cccattgtgg gcaagctgag cgaccgcacc acgtcccgct
    11581 acgggatgcg caagccctgg atggtcggcg gaacgatcgt cgccatcgtt ggactcctcg
    11641 tgaccgcgac atcgacgacg atgccactga ttctgctggg atactgcatc gccagtttcg
    11701 ggctaaacgc gaccgcggcc acgctcatcc cgctactccc agatcaggtt ccgcaccgac
    11761 agcggggcag agtctccggc atcttgggca tgggtctgcc tgtcggagcc gtcggcggcg
    11821 ccgggctcgc ccaggcgttc gccgccaacc cgttgtggat gttcctggcc ccagcggtcg
    11881 tactggcgat cggcgttgcg gtgctcgtga cgaggtacga ggaccgtacc ctcgctgcgg
    11941 cccaggcggc gagactaccg cgctacgggg tgcgcgagtt cttggccagc ttctgggtca
    12001 gtcccaagcg gcacccggca ttcgcctgga cctggctgag tcgtttcctg gtcttcatgg
    12061 gcgtggcgac tctcgtcacc ttccagtccg tgtacctcat cgaacatctc gggttccccc
    12121 tcatcgccgt tccgatgctc gtggccctcg ccacctcggt ccactacttg ttcgtgttcg
    12181 cggccagccc ggtcgccggc tggctctccg accggatgga ccggcggaag atcttcgtgg
    12241 cgctcgcagc cctgttctac gcggtgggac taggtgtgat cgcgtccgcg gaatcgttca
    12301 cggcattcct cattggcatg gctatcaccg gcattgggga gggcgcgtat tcggcagtcg
    12361 acctcgcgtt ggtgaccgac gtcttgccca acccggatga cgccggcaag gagatgggcg
    12421 tgttcgccgt agccaacacc ctcccgccgg cgatcgcgcc agccatcgcg ccggctctcc
    12481 tcgccattcc gtttctgggc aggggcgacg gtgggaactt cgtcgcgctt ttcgcggtcg
    12541 ccgccctact ggcggtgctc ggcgccgtcg ccatcagccc tgtgcgcacg gcccgcgatc
    12601 ggtcaaatgt agcggtgtaa
//
