LOCUS       BGC0001440             13771 bp    DNA     linear   BCT 09-MAY-2017
DEFINITION  Kitasatospora cystarginea strain JCM 7356 alpha/beta hydrolase gene,
            complete cds; cys gene cluster, complete sequence; and 3-oxoacyl-ACP
            synthase gene, complete cds.
ACCESSION   BGC0001440
VERSION     BGC0001440
KEYWORDS    .
SOURCE      Kitasatospora cystarginea
  ORGANISM  Kitasatospora cystarginea
            Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
            Kitasatospora.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-08 01:38:13
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 13771
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..861
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1036:alpha/beta hydrolase fold protein (Score: 104.9;
                     E-value: 1e-31)"
                     /gene_kind="biosynthetic-additional"
                     /product="alpha/beta hydrolase"
                     /protein_id="ARO49558.1"
                     /sec_met_domain="Abhydrolase_6 (E-value: 9.2e-18, bitscore:
                     55.0, seeds: 455, tool: rule-based-clusters)"
                     /sec_met_domain="PF00561 (E-value: 8e-20, bitscore: 60.9,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQKARIDGTDIAYEEAGSGETVVLVHAGCADRRMWQEQFAALSER
                     YRVIRYDWRGRGASGDAVGSFAHHRDLLALMDRLQVRRAAVVGASDGGRIALDALLTAP
                     HRFRALGLIAAGLSGHQWPASLVDRARRQVHSLVPSERLRHYQQGAAERIEPGDLDAVA
                     EAEAELLVAGPDRTRAVLPPRVWELAVAMDRLTRERMWGGPRFTEQQLCPPAKERLAEV
                     AVPTLVVAGLADAPEILAASALLAEGIPGARRLDLPDTGHLPPVERPQLVTTALTDFLA
                     TTMPW"
     source          1..13771
                     /culture_collection="NRRL:B-16505"
                     /db_xref="taxon:58350"
                     /mol_type="genomic DNA"
                     /organism="Kitasatospora cystarginea"
                     /strain="JCM 7356"
                     /type_material="type strain of Kitasatospora cystarginea"
     protocluster    1..13771
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[924:8486]"
                     /cutoff="10000"
                     /detection_rule="(HMGL-like and AMP-binding)"
                     /neighbourhood="10000"
                     /product="betalactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      925..8486
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(HMGL-like and AMP-binding)"
                     /neighbourhood="10000"
                     /product="betalactone"
                     /protocluster_number="1"
     cand_cluster    1..13771
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(HMGL-like and AMP-binding)"
                     /kind="single"
                     /product="betalactone"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..13771
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0001440"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..13771
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="betalactone"
                     /region_number="1"
                     /rules="(HMGL-like and AMP-binding)"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            complement(925..2577)
                     /gene="cysA"
     CDS             complement(925..2577)
                     /codon_start=1
                     /gene="cysA"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     betalactone: HMGL-like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1271:2-isopropylmalate synthase (Score: 477.1;
                     E-value: 1e-144)"
                     /gene_kind="biosynthetic"
                     /product="2-IPM synthase"
                     /protein_id="ARO49559.1"
                     /sec_met_domain="HMGL-like (E-value: 7.2e-77, bitscore:
                     247.8, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAVHDGGTRLRSVPAHRGGAAAHRGGARMPSARYAPLRTPDLPD
                     RTWPGRTLTAAPRWLSTDLRDGNQALLEPMSPDRKLRMFELLLAMGYREIEVGFPSASQ
                     TDFDFVRRLIREERVPDGVRISVLTQAREALIERTVESLVGARAATVHLYNATSPLFRR
                     VVFGTSREECRDLAVRGTGQLMKYADQLLGDCDLGYQYSPELFNETEPDFALEVCEAVM
                     DVWQPGPGREIVLNLPSTVERTTPNVYADQVEWLSRSLTRREYVALSVHPHNDRGTAVA
                     AAELGVLAGADRIEGCLFGNGERTGNVCLVTLGLNLHAQGIDPGIDFSDIDRVRRTVED
                     CTGLPVPARHPYGGDLVYTSFSGSHQDAIKKGFAAMERQAAEDGVPVERTAWTVPYLPI
                     DPADVGRSYEAVIRVNSQSGKGGVAYLMRQEHGLELPRRLQIEFSRIVQEHADAIGGEI
                     TAERLGALFSAEYLEVAAEHPEAEGAVDPLRLEVLTCAEQTRGTSTDGAAEVVAYAHCS
                     VDGRNLWGVGRGADSATARRRAVASAVGRSRRV"
     misc_feature    925..10360
                     /note="cys gene cluster"
     gene            complement(2574..4013)
                     /gene="cysB"
     CDS             complement(2574..4013)
                     /codon_start=1
                     /gene="cysB"
                     /gene_functions="transport (smcogs) SMCOG1005:Drug
                     resistance transporter, EmrB/QacA (Score: 418.3; E-value:
                     5.1e-127)"
                     /gene_kind="transport"
                     /product="MFS transporter"
                     /protein_id="ARO49560.1"
                     /transl_table=11
                     /translation="MNRLRGNPWAILIVLSLGFFMTLLDLTIVNIAIPSMIDDLHASFD
                     AVLWSLNGYILVLAALLITAGRLGDLRGPRQLFALGVAVFTLASLVCGLVQNPAELIAA
                     RLVQGVGAALLVPQTMTLIVRAFPAERRGAALGVWGAVAGVAAVAGPALGGLLVSELGW
                     RWIFFVNVPVGAVVLATVFTVVPDLRQGGARHRLDFVGVALSSAAVFCLAFALTEGERY
                     HWNAVIWGLLFAAAALFAAFAAQQRRRQAEEPLVPFQLFHDRNYTVMNAVSASVSIGVL
                     GLMLLLSLYFQSVLHFSALKAGLALAPASAVSMALAPFTGRLSDRIDGKFVLLAGMVLT
                     AVGMLWSALVMGPATPWAAFVAPMVVVGAGNGCLIAPMAAVAMRGVDPAVVGAASGVLN
                     TVRQLGSVVGTAAVGALVQVQTADARSGVSTMRTSMALPITVILLGAVACAAARRDVSA
                     KPAAEPASAGAAAQDCGRSGG"
     gene            complement(4010..5188)
                     /gene="cysC"
     CDS             complement(4010..5188)
                     /NRPS_PKS="Domain: AMP-binding (55-219). E-value: 8.6e-13.
                     Score: 39.4. Matches aSDomain:
                     nrpspksdomains_cysC_AMP-binding.1"
                     /codon_start=1
                     /gene="cysC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     betalactone: AMP-binding"
                     /gene_kind="biosynthetic"
                     /product="AMP-dependent synthetase"
                     /protein_id="ARO49561.1"
                     /sec_met_domain="AMP-binding (E-value: 1e-12, bitscore:
                     36.7, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIGQIPTERLLTHARTLAEAGGAPVPPAARQLADLAVTGPAEIYA
                     LTGAAARSAGSVLMSSGGTTGRPKLTYVPHGMGLTRLLEHWRPLRPGNVLLNLFNAGRM
                     WGSHYYMQTLAERSGCTVIPSGPYSPAEVAGWLPMLAEVGVDALAGTPTGLADFAQGVI
                     DAGGTLPVRTVIWMAEPWTGNKRELVRQAFPEAGLWGNYGSVETWVMCTNQPGCDETTL
                     HLLPDQVMEPDEDGALLSRVGEGWTVPVVRYRLGDRVAPVECRCGRPDALRVLGRADDS
                     VTLRSALFKVSELVDLVRGEPGVLEAQLRLTRSADSPKAASALTLEFTGTADAEAVRGR
                     LIGGFYHLAAVARQYPDALRARRVERLTRIERTNKVPAMVWQQAETDGAGVA"
     aSDomain        complement(4532..5023)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_cysC_AMP-binding.1"
                     /evalue="8.60E-13"
                     /label="cysC_AMP-binding.1"
                     /locus_tag="cysC"
                     /protein_end="219"
                     /protein_start="55"
                     /score="39.4"
                     /specificity="consensus: X"
                     /tool="antismash"
                     /translation="SVLMSSGGTTGRPKLTYVPHGMGLTRLLEHWRPLRPGNVLLNLFN
                     AGRMWGSHYYMQTLAERSGCTVIPSGPYSPAEVAGWLPMLAEVGVDALAGTPTGLADFA
                     QGVIDAGGTLPVRTVIWMAEPWTGNKRELVRQAFPEAGLWGNYGSVETWVMCTNQPGCD
                     E"
     gene            complement(5185..6186)
                     /gene="cysD"
     CDS             complement(5185..6186)
                     /codon_start=1
                     /gene="cysD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RimK"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Dala_Dala_lig_C"
                     /gene_kind="biosynthetic-additional"
                     /product="ATP-dependent carboxylate-amide ligase"
                     /protein_id="ARO49562.1"
                     /sec_met_domain="RimK (E-value: 1.6e-14, bitscore: 43.3,
                     seeds: 4, tool: rule-based-clusters)"
                     /sec_met_domain="Dala_Dala_lig_C (E-value: 1.7e-09,
                     bitscore: 26.8, seeds: 12, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTAPAAPTRRLCWIYPDRGTDRMRDKEWKHVWGIYREVAREGGW
                     ALSLHKPEEVAVDVCGPGGPKVYLDGERVTPEDTVFVTSLWSLPHHTVDVCNQLYLYTI
                     LEQAGFYLPIPPHLSFIANDKAATMLHLADSPLPQVPTVRIGTGRDTAQRSYEAALASL
                     SYPAIVKPAYWGMGMGVCLVRNAEELKGVAGIASGADTALVCQPYLGEGINDFRVWVVG
                     GKPHTVLRRIPKGASLTANLSSGGGMEHVPLPPELAETVDYVAARMPMPYIAVDFLWDG
                     ERFWLSEVEPDGAVGFADSEQTEREQRKVIADRFAAYADAHRQFLNRKDAIR"
     gene            complement(6203..7018)
                     /gene="cysE"
     CDS             complement(6203..7018)
                     /codon_start=1
                     /gene="cysE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1036:alpha/beta hydrolase fold protein (Score: 124.2;
                     E-value: 1.3e-37)"
                     /gene_kind="biosynthetic-additional"
                     /product="methylesterase"
                     /protein_id="ARO49563.1"
                     /sec_met_domain="Abhydrolase_6 (E-value: 6.8e-26, bitscore:
                     81.6, seeds: 455, tool: rule-based-clusters)"
                     /sec_met_domain="PF00561 (E-value: 1.9e-25, bitscore: 79.3,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAELLLEDGQSIHYQETGKGSPVLLVHGLGAPSAFLAATAEGLAR
                     DHRVVTFDLRGHGRTPLGTGPVGIDRCAADLHAVAGKLDLRAVTLVGWSLGATVAYRYL
                     ERYGAQRVARLVSVEQSPYLLYEDGWEHAAFGRLTAADAETVRQNLAGTDRAVAADQVA
                     GYFAEGTVPDPDLLARLADAVATCSPAARQQLWQDVVRQDWRERLAALPVPVLFVHGAR
                     SRIYPSAVGSRLADTVPGARLEVFENSGHLPFLEEPERFQRTIRSWVAR"
     gene            complement(7011..8486)
                     /gene="cysF"
     CDS             complement(7011..8486)
                     /NRPS_PKS="Domain: AMP-binding (7-382). E-value: 1.9e-64.
                     Score: 209.6. Matches aSDomain:
                     nrpspksdomains_cysF_AMP-binding.1"
                     /codon_start=1
                     /gene="cysF"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     betalactone: AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002:AMP-dependent synthetase and ligase (Score:
                     243.5; E-value: 5.8e-74)"
                     /gene_kind="biosynthetic"
                     /product="AMP-dependent synthetase"
                     /protein_id="ARO49564.1"
                     /sec_met_domain="AMP-binding (E-value: 4.4e-53, bitscore:
                     169.6, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLYEALRDIAARRPDARAVTTADGASASYAELLDLIDRTAAGLRG
                     HGVGAGDVIACSLRNSIRYVALILAAARIGARYVPLMSNFDRADIATALRLTGPRMIVT
                     DHQREFPDQAPPRVRLETLEAATASPREAGERYDGLFRSLWTSGSTGFPKQMVWRQDRF
                     LRERRRWLADTGITADDVFFCRHTLDVAHATDLHVFAALLSGAELVLADPDAAPDVLLR
                     QIAERRATAMSALPRHYEEYVRAAAGRPAPDLSRLRRPLCGGAYVSAAQLTDAAEVLGI
                     HIRQIYGSTEFGLAMGNMSDVLQAGVGMVPVEGVGVRLEPLAADRPDLGELVLISDCTS
                     EGYVGSDEANARTFRGEEFWTGDVAQRGPDGTLRVLGRVTETLAAAGGPLLAPVLDEEI
                     AAGCPVLETAALPAHPDRYSDEVLLVLHPDPDRPEQELRKAVAEVLDRHGLRASIRLTD
                     DIPHTPVGKPDKPALRRRWESGALGPVGEWHHG"
     aSDomain        complement(7341..8465)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_cysF_AMP-binding.1"
                     /evalue="1.90E-64"
                     /label="cysF_AMP-binding.1"
                     /locus_tag="cysF"
                     /protein_end="382"
                     /protein_start="7"
                     /score="209.6"
                     /specificity="consensus: X"
                     /tool="antismash"
                     /translation="DIAARRPDARAVTTADGASASYAELLDLIDRTAAGLRGHGVGAGD
                     VIACSLRNSIRYVALILAAARIGARYVPLMSNFDRADIATALRLTGPRMIVTDHQREFP
                     DQAPPRVRLETLEAATASPREAGERYDGLFRSLWTSGSTGFPKQMVWRQDRFLRERRRW
                     LADTGITADDVFFCRHTLDVAHATDLHVFAALLSGAELVLADPDAAPDVLLRQIAERRA
                     TAMSALPRHYEEYVRAAAGRPAPDLSRLRRPLCGGAYVSAAQLTDAAEVLGIHIRQIYG
                     STEFGLAMGNMSDVLQAGVGMVPVEGVGVRLEPLAADRPDLGELVLISDCTSEGYVGSD
                     EANARTFRGEEFWTGDVAQRGPDGTLRVLGRVTET"
     gene            complement(8544..9230)
                     /gene="cysG"
     CDS             complement(8544..9230)
                     /codon_start=1
                     /gene="cysG"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089:methyltransferase (Score: 66.1; E-value:
                     5.6e-20)"
                     /gene_kind="biosynthetic-additional"
                     /product="SAM-dependent methyltransferase"
                     /protein_id="ARO49565.1"
                     /transl_table=11
                     /translation="MSQTASPRIELWNPETYDALRRQLIPSFDLLYGSAVSVVAMSVPA
                     TARILDLGAGTGLLGAALRERLPDAELLLQDRSQAMLEQARQRFADDDQVAIRVADHLD
                     ELPAGPFDAVVSALSIHHLEHQDKQDLFTRIRKILRPGGIFVNVEQVLAPTSELEKMYD
                     RQHEAHVLASDTPAEEWAAGRERMKHDIPIDVETQIQWLRDAGFTTADCLAKDWRFATY
                     AGWNGS"
     gene            complement(9431..10360)
                     /gene="cysH"
     CDS             complement(9431..10360)
                     /codon_start=1
                     /gene="cysH"
                     /gene_functions="regulatory (smcogs) SMCOG1014:LysR family
                     transcriptional regulator (Score: 126.6; E-value: 1.9e-38)"
                     /gene_kind="regulatory"
                     /product="LysR family transcription regulator"
                     /protein_id="ARO49566.1"
                     /transl_table=11
                     /translation="MYRNLDVSQLRTLVTIMSAGGFRRAAEKLSLTQPAVSQHIRRLEA
                     VLGGPVFARTGRQLELTTAGQELYGYARRIVALNDEAVARITAPHEVTEFSVGLCPQLE
                     GALPDFLSGLVKLLPGVRPTIHLGLGEPLAARVAAGELDVALLLRPPREDGTRFLGAMR
                     LAWFGADPGSAGGPLSLALCGEPCNLRRHLLDTLGAHDLPWQIAYEGPELAGLRAAVSV
                     GLGVTCLLANADELWGLRRLPAGALPVPEPMPVSLAVAPQVPAGVVEVALRAAQRALRA
                     YPITGVEPVPGHPQQAARRPGTDRVLVG"
     CDS             complement(12743..13771)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) fabH"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1084:3-oxoacyl-(acyl carrier protein) synthase III
                     (Score: 360.9; E-value: 8.8e-110)"
                     /gene_kind="biosynthetic-additional"
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="ARO49567.1"
                     /sec_met_domain="fabH (E-value: 1.4e-106, bitscore: 345.6,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNTTDVRAVVTGIGSCLPPDVVTNDDLSRVLDTTHDWIFSRTGIA
                     ERRAVRPGTSTGDLGTAAGAAALESAGRRDCDLVLLATTTPDQRCPATAPQIAARLGLA
                     GVPAFDLSAVCSGFVYGLSVAGAMIGAGSYESVLLIGAETYSTIVDPADRGTAVLFGDG
                     AAAVVLERGLPGTPGELLHVELGSDGAGADLIAIPGGGSRAPHLAADSAARYFRMDGRE
                     VYAQAVAAMTRSARSVAEAVGWRTEQIDAFVGHQANARILRAVGRRLGLAEERVVCRLA
                     EVGNTAGASIPLALAQAAADDRLSKGDRVLMTAFGGGLTWGSAAMVWSGVQPVQADGPC
                     PV"
ORIGIN
        1 gtgcagaagg cacggatcga tgggacggac atcgcgtacg aggaggccgg gtccggggag
       61 accgtcgttc tggtccacgc cgggtgtgcg gaccggcgga tgtggcagga gcagttcgcg
      121 gcgctctccg agcggtaccg cgtgatccgt tacgactggc gggggcgggg cgcttcgggg
      181 gacgcggtcg gctccttcgc ccatcaccgt gatctgctcg cgctgatgga ccggttgcag
      241 gtccggcggg cggcggtggt cggcgcgtcc gacggcggga ggatcgcgct ggacgcgctg
      301 ctcaccgctc cgcaccggtt ccgggccctc ggcctgatcg cggccgggct gtccggtcac
      361 cagtggcccg cctccctggt cgaccgggcg cggcggcagg tgcacagcct cgtgccgagc
      421 gagcggctgc gccactacca gcagggcgcc gccgagcgga tcgagcccgg tgacctcgac
      481 gccgtcgccg aggcggaggc cgagctgttg gtggccggtc ctgaccgcac ccgggccgtg
      541 ctgccgcctc gggtctggga gctggccgtg gccatggacc ggctcacccg ggaacggatg
      601 tggggcggcc cgcggttcac cgagcagcag ctgtgcccgc cggccaagga gcggctggcc
      661 gaggtggcgg tgccgacact ggtcgtcgcg gggctggcgg acgcaccgga gatcctggcc
      721 gcatccgccc tgctggccga gggcattccc ggggcccgtc gactcgacct ccccgacacc
      781 ggccatctgc cgccggtgga gcggccgcag ctggtcacca ccgcgctgac ggacttcctc
      841 gcaacgacca tgccctggta ggtggttggg gtgcaggggc ggggagagcg gctgtgacgc
      901 ccagccaggc ctccccgccc gccctcagac gcgccgggac ctgccgaccg cgctcgccac
      961 cgcccgccgc cgtgcggtcg cggagtcggc gccgcggccg accccccaca ggttccgccc
     1021 gtcgaccgag cagtgggcgt aggcgacgac ctccgccgcc ccgtccgtcg aggtgccccg
     1081 cgtctgctcc gcacacgtga ggacctccag ccggagcggg tccaccgccc cctccgcctc
     1141 cgggtgctcc gcagcgacct ccaggtactc cgccgagaac agcgcgccca gtcgctccgc
     1201 cgtgatctcc ccgccgatcg cgtcggcgtg ttcctggacg atccgggaga actcgatctg
     1261 gagccgccgc ggcagctcca gtccgtgctc ctgccgcatc aggtacgcca ctccgccctt
     1321 gccggactgg ctgttgaccc ggatgaccgc ctcgtaggag cggcccacgt ccgccgggtc
     1381 gatcggcagg tacggcaccg tccaggcggt ccgctccacc ggcaccccgt cctcggcggc
     1441 ctgccgttcc atggccgcga agcccttctt gatcgcgtcc tggtgcgacc cggagaagga
     1501 ggtgtacacc aggtcgccgc cgtatgggtg gcgggcgggc accgggaggc cggtgcagtc
     1561 ctcgaccgta cggcggacgc ggtcgatgtc ggagaagtcg atgccggggt cgatgccctg
     1621 ggcgtgcagg ttgaggccga gggtgaccag gcagacgttg ccggtccgct cgccgttgcc
     1681 gaacaggcag ccctcgatgc ggtcggcgcc ggccagtacg ccgagttcgg cggcggccac
     1741 ggcggtcccc cggtcgttgt gcggatgcac cgagagggcg acgtactccc gacgggtcag
     1801 gctgcggctc agccactcga cctggtccgc gtagacgttg ggagtggtgc gttccaccgt
     1861 ggaaggcagg ttgagcacga tctcgcggcc cggccccggc tgccagacgt ccatgaccgc
     1921 ctcgcagacc tccagcgcga agtccggctc ggtctcgttg aacagttccg gcgagtactg
     1981 gtagcccagg tcgcagtcgc cgaggagctg gtcggcgtac ttcatcagct gtccggtacc
     2041 gcgcaccgcc aggtcccggc actcctcccg gctcgtcccg aagaccaccc ggcggaacag
     2101 cggtgacgtg gcgttgtaca ggtggacggt ggccgcgcgg gctcccacca ggctctccac
     2161 ggtgcgttcg atcagcgcct cgcgggcctg ggtgagcacg gagatgcgca ctccgtcggg
     2221 cacccgctcc tcgcggatca gccggcggac gaagtcgaag tcggtctggc tggcgctggg
     2281 aaagccgacc tcgatctccc ggtaccccat cgccagcagc aactcgaaca tccgcagctt
     2341 gcggtcgggg ctcatcggct ccagcagtgc ctggttgccg tcgcgcagat cggtggagag
     2401 ccagcgggga gccgccgtca gcgtccggcc gggccaggtg cggtccggca ggtccggtgt
     2461 ccgcagcggc gcgtaccgcg cggacggcat ccgcgcacca ccccggtggg ccgctgcacc
     2521 accccggtgg gccggtacgg accgcaaccg cgtgccgccg tcgtgaaccg ccgtcacccg
     2581 ccgctccttc cgcagtcctg agctgccgcc cccgccgaag cgggttcggc ggccggcttc
     2641 gccgagacgt cccgccgggc cgccgcgcag gccacggcac cgagcaggat gaccgtgatg
     2701 ggcagcgcca tcgaggtgcg catcgtgctc accccgctgc gcgcgtccgc ggtctgcacc
     2761 tgtaccaggg cgccgaccgc ggcggtgccg acgaccgacc ccaactgccg tacggtgttg
     2821 agcactccgg aggcggcgcc gaccaccgcc gggtcgacgc cgcgcatcgc caccgcggcc
     2881 atcggcgcga tcaggcagcc gttgcccgct cccaccacga ccatgggggc gacgaacgcc
     2941 gcccacggcg tcgccgggcc catcaccagg gcgctccaca gcatcccgac cgccgtcagc
     3001 accatgcccg cgagcagcac gaacttgccg tcgatgcggt cggagaggcg gccggtgaag
     3061 ggggcgagcg ccatcgagac ggcggaggcc ggggcgagcg cgagcccggc cttcagcgcg
     3121 ctgaagtgca gcacggactg gaagtacagc gagagcagca gcatcagccc gagcacgccg
     3181 atcgagaccg aggccgaaac ggcgttcatg accgtgtagt tgcggtcgtg gaagagctgg
     3241 aacggcacca gcggctcctc cgcctgccgc cggcgctgct gggcggcgaa ggcggcgaac
     3301 agcgccgcgg cggcgaacag cagcccccag atgacggcgt tccagtggta gcgctcgccc
     3361 tcggtcaacg cgaaggcgag gcagaagacc gccgccgagg acagcgccac tcccacgaag
     3421 tccagccggt gccgggcgcc gccctggcgg aggtccggga ccacggtgaa gacggtggcc
     3481 aggacgaccg cgccgaccgg cacgttcacg aagaagatcc agcgccagcc cagttcactg
     3541 accagcaggc cgccgagcgc cgggccggcc accgcagcga caccggccac cgcgccccag
     3601 accccgagcg ccgcgccgcg ccgctcggcc ggaaaggcgc gcacgatcag ggtcatggtc
     3661 tgcggcacga gcagggccgc gcccactccc tgcaccagcc gggcggcgat gagttcggcg
     3721 gggttctgca ccagcccgca gaccaggctc gccagggtga acacggcgac gcccagcgcg
     3781 aagagctggc gcggaccgcg caggtcgccc agccggcccg cggtgatcag cagtgcggcg
     3841 agcaccagga tgtagccgtt cagcgaccac agcaccgcgt cgaaggaggc gtgcaggtcg
     3901 tcgatcatgc tgggaatggc gatgttgacg atcgtcaggt ccagcagggt catgaagaac
     3961 ccgagcgaca ggacgatcag gatcgcccac ggattgccgc ggagccggtt catgcgacac
     4021 ctgccccgtc ggtttcggcc tgctgccaga ccatcgcggg caccttgttg gtgcgctcga
     4081 tccgggtgag ccgctccacc cgccgggccc gcagggcgtc ggggtactgc ctggccaccg
     4141 ccgcgaggtg gtagaacccg ccgatcagcc ggccgcgcac cgcctccgcg tcggcagtcc
     4201 cggtgaactc gagcgtcagc gcgctggccg ccttgggcga gtcggcgctc cgggtgagcc
     4261 gcaactgggc ctccagcacg ccgggttcac cgcggaccag gtcgaccagc tcgctcacct
     4321 tgaacagcgc gctgcgcagg gtcaccgagt cgtccgcccg gcccagcacc cgcagggcgt
     4381 cgggccggcc gcagcggcac tcgacggggg ccacccggtc gcccagccgg tagcgcacca
     4441 cgggcaccgt ccagccctcg ccgacccggc tgagcagcgc gccgtcctcg tccggttcca
     4501 tcacctggtc gggcagcagg tgaagggtcg tctcgtcgca gcccggctgg ttggtgcaca
     4561 tcacccaggt ctccaccgag ccgtagttgc cccacagccc ggcctcgggg aacgcctggc
     4621 ggaccagctc gcgcttgttg ccggtccacg gttcggccat ccagatgacg gtgcgcaccg
     4681 ggagggtgcc gccggcgtcg atgacgccct gggcgaagtc ggcgagcccc gtcggggtgc
     4741 ccgccagggc gtccaccccg acctccgcca gcatcggcag ccagccggcc acctccgccg
     4801 gggagtaggg tccggacggg atgaccgtgc agccgctccg ctcggccagc gtctgcatgt
     4861 agtagtgcga tccccacatc cggccggcgt tgaagaggtt gagcagcacg ttgcctgggc
     4921 gcagcggccg ccagtgctcc aggagccggg tgagtcccat gccgtgcggg acgtaggtca
     4981 gcttgggacg gccggtggtg ccgccactgg acatcagcac gctgccggcg ctgcgcgccg
     5041 ccgcaccggt cagcgcgtag atctcggcgg ggcccgtgac ggccaggtcg gcgagctgcc
     5101 gcgcggccgg ggggacgggc gccccgccgg cctcggccag ggtgcgcgcg tgtgtcagca
     5161 gccgttcggt cggtatctgg ccgatcatcg gatggcgtcc ttccggttca ggaactgccg
     5221 gtgtgcgtcc gcgtacgcgg cgaaccggtc ggcaatgacc ttccgctgct cgcgctcggt
     5281 ctgctccgag tcggcgaacc cgaccgcgcc gtccggctcc acctccgaga gccagaagcg
     5341 ctcgccgtcc cagaggaagt cgacggcgat gtacggcatc ggcatccgcg ccgccacgta
     5401 gtcgacggtc tcggcgagtt cgggcggcag cggcacgtgc tccatgccgc cgcccgaact
     5461 caggttcgcg gtgagcgacg ctcccttggg gatgcggcgc agcacggtgt gcggcttgcc
     5521 gcccacgacc cacacccgga agtcgttgat gccctcaccg aggtagggct ggcagaccag
     5581 ggcggtgtcc gcgccggacg cgatgccggc cacgcccttc agttcctcgg cgttgcgcac
     5641 caggcagacg cccatgccca tgccccagta ggcgggtttg acgatggccg ggtagctgag
     5701 ggaggccagc gccgcctcgt agctgcgctg cgcggtgtcg cggccggtgc cgatccggac
     5761 ggtgggcacc tgcggcagcg ggctgtccgc gaggtggagc atggtcgccg ccttgtcgtt
     5821 ggcgatgaac gacaggtggg gcgggatggg caggtagaag ccagcctgtt cgaggatcgt
     5881 gtacaggtag agctggttgc agacgtccac ggtgtggtgg ggcagcgacc agagcgaggt
     5941 cacgaacacg gtgtcctcgg gggtgacgcg ctcgccgtcc aggtagacct tggggccgcc
     6001 cgggccacag acgtccacgg cgacctcttc cggcttgtgc agggagagcg cccagccgcc
     6061 ctcacgggcc acctcccggt agatccccca gacgtgcttc cattccttgt cccgcatccg
     6121 gtcggtgccg cggtccgggt agatccagca cagccggcgg gtcggggcgg caggggcagt
     6181 ggtcatcagc agtccgcctt cgtcatcggg ccacccagga gcggatcgtc cgctggaagc
     6241 gctccggctc ctccaggaac ggcaggtggc cgctgttctc gaacacctcg agtcgggccc
     6301 cgggcacggt gtccgcgagc cggctcccga cggcgctggg gtagatccgg ctccgcgcgc
     6361 cgtgcacgaa gagcaccggc accggcagtg ccgccagccg ttcgcgccag tcctggcgca
     6421 ccacgtcctg ccagagctgc tgtctggcgg cgggcgagca ggtggcgacc gcgtcggcga
     6481 gcctcgccag taggtcgggg tcggggacgg tgccctcggc gaagtaaccg gccacctggt
     6541 cggccgcgac ggcccggtcc gtgccggcga ggttctgccg gacggtctcc gcgtccgcgg
     6601 cggtgagccg gccgaacgcc gcgtgttccc agccgtcctc gtacagcagg tacgggctct
     6661 gctccaccga caccagccgg gcgacgcgct gtgcgccgta ccgctccagg tagcggtagg
     6721 cgacggtggc gccgagtgac cagccgacca gggtcaccgc ccgcaggtcc agcttcccgg
     6781 ccaccgcgtg caggtccgcg gcgcagcggt cgatcccgac cggtccggtg cccagtgggg
     6841 tccggccgtg gccgcgcagg tcgaaggtga cgacccggtg gtcgcgggcc aggccctcgg
     6901 cggtggcggc gaggaaggca gacggtgcgc cgagcccgtg caccagcagt accggcgaac
     6961 ctttcccggt ctcctggtag tggatcgact gtccatcttc caggaggagt tcagccatgg
     7021 tgccactcgc cgaccggacc gagggcaccg ctctcccagc ggcggcgcag ggccggcttg
     7081 tcgggcttgc cgaccggagt gtgcgggatg tcgtcggtca gccggatcga ggcccgcagc
     7141 ccgtgccggt ccagcacctc ggcgacggcc ttgcggagtt cctgctccgg ccggtccggg
     7201 tccgggtgca gcaccagcag cacctcgtcg gagtaccggt ccgggtgggc cggcagggcg
     7261 gccgtctcca gcaccgggca gccggctgcg atctcctcgt ccagcacggg ggcgagcagc
     7321 ggaccgccgg cggcggccag ggtctcggtg acccggccga gcacccgcag cgtcccgtcc
     7381 gggccccgct gggcgacgtc tccggtccag aactcctcac cccggaaggt gcgggcgttc
     7441 gcctcgtcgc tgccgacgta tccctcgctg gtgcagtccg agatgagcac cagctcgccg
     7501 aggtcgggcc ggtcggccgc gagcggttcc agccggacgc cgacgccctc gacggggacc
     7561 atgccgacgc cggcctggag cacgtcggac atgttgccca tggccaggcc gaactcggtg
     7621 gagccgtaga tctgccggat gtggattccc agcacctcgg cggcgtcggt cagttgcgcg
     7681 gcgctgacgt aggctccgcc gcacagcggg cggcgcagtc gcgacaggtc cggcgccggg
     7741 cggcccgcgg ccgcccgcac gtactcctcg tagtgccgcg gcagggcgct catcgcggtg
     7801 gcccgccttt cggcgatctg ccgcagcagg acgtccggcg cggcgtccgg gtcggccagc
     7861 accagttccg cgccggacag cagtgcggcg aagacgtgca ggtccgtggc gtgcgcgacg
     7921 tcgagggtgt gccggcagaa gaagacgtcg tcggcggtga tgccggtgtc cgccagccag
     7981 cggcggcgct cgcgcaggaa gcggtcctgc cgccagacca tctgcttggg gaagccggta
     8041 gaccccgagg tccacagtga gcggaacagc ccgtcgtacc gctcgcccgc ctcccggggc
     8101 gaggccgtgg cggcttccag ggtctccagc cggacccgcg gcggggcctg gtcggggaac
     8161 tcccgctggt ggtcggtgac gatcatccgg ggcccggtga ggcgcagtgc cgtggcgatg
     8221 tcggcgcggt cgaagttgct catcagcggc acgtagcggg cgccgatgcg ggccgcggcc
     8281 aggatgagcg ccacatagcg gatgctgttg cgcagcgagc aggcgatcac gtccccggcg
     8341 cccacgccgt gcccgcgcag cccggcggcc gtgcggtcga tcaggtctag cagttccgcg
     8401 tagctcgcgc tcgccccgtc cgcggtggtg acggctcgcg cgtcgggccg ccgggcggcg
     8461 atgtcacgca gcgcttcgta aagcacggtt cacccttccg agcccgtccg cggttcgtgg
     8521 tgtgcggagc gcgccgtggg cggtcacgat ccgttccagc ccgcgtaggt ggcgaaacgc
     8581 cagtccttcg ccaggcagtc ggcggtggtg aagcccgcgt cgcgcagcca ctggatctgg
     8641 gtttcgacgt cgatgggaat gtcgtgcttc attcgctcac ggccggctgc ccattcctcc
     8701 gccggagtgt cggacgcgag cacgtgcgcc tcgtgctgcc ggtcgtacat cttctccagc
     8761 tcggacgtgg gcgcgagcac ctgctcgacg ttgacgaata ttccgccggg gcgcaggatc
     8821 ttccggatcc gcgtgaacag gtcctgcttg tcctggtgtt ccaggtggtg gatggagagg
     8881 gcggacacca cggcgtcgaa gggcccggcg gggagctcgt cgaggtggtc ggccacccgg
     8941 atggccacct ggtcgtcgtc ggcgaaccgc tggcgggcct gctccagcat cgcctgcgag
     9001 cggtcctgga ggagcagttc ggcgtcgggg agccgctcgc gcagcgcggc gccgagcagg
     9061 ccggtgccgg ctccgaggtc caggatgcgc gccgtggccg gcactgacat ggccaccacg
     9121 ctcaccgcgc tgccgtagag gaggtcgaac gacgggatga gctgcctgcg cagcgcgtca
     9181 taggtttccg gattccacag ttcgattctg ggggaagcgg tctgggacat gcctctgttc
     9241 ctttcctcac aagacctggg gaatggccgg ggaaaccctc ccggaaattc cacgaattac
     9301 accggtggtg cgggccgaaa atgatttcaa gatcacagca tgttcacgcc aaggctaagt
     9361 gtccgccgct caggtgcgca aatcactctt ccttatccgt ttgataagcg ccggccattc
     9421 gactcaccgg tcagccgacc aggacgcggt cggtgcccgg gcgcctggcg gcctgctggg
     9481 ggtggccggg gaccggctcc accccggtga tcgggtaggc ccgcagggcg cgctgggccg
     9541 cgcggagggc cacctcgacc acccccgcgg ggacctgggg agccaccgcc agcgagacgg
     9601 gcatcggctc ggggaccggc agcgcaccgg cgggcagccg gcgcagcccc cacagctcgt
     9661 ccgcgttggc cagcaggcag gtcaccccca ggccgacgga gaccgcggcg cgcagcccgg
     9721 cgagttccgg cccctcgtag gcgatctgcc agggcaggtc gtgggcgccg agggtgtcca
     9781 ggaggtgccg ccgcagattg cacggttcac cgcacagcgc gagcgacagc ggcccgccgg
     9841 ccgaccccgg gtcggcgccg aaccaggcca gccgcatcgc cccgaggaac ctggtcccgt
     9901 cctccctggg cgggcgcagc agcagggcga cgtcgagctc tccggccgcc acccgtgccg
     9961 ccagcggctc gccgagtccc aggtggatgg tcgggcggac tccgggcagg agtttcacca
    10021 ggccggagag gaagtccggc agcgcgccct ccagttgggg gcagaggccg acactgaact
    10081 ccgtcacctc gtgcggtgcg gtgatccgcg ccacggcctc gtcgttgagc gcgacgatcc
    10141 gacgcgcgta gccgtacaac tcctgcccgg ccgtggtgag ttcgagctgg cgcccggttc
    10201 gggcgaagac cggtccgccc agcaccgcct ccagccgccg gatgtgctgg ctcacggcgg
    10261 gctgggtcag cgacagcttc tccgccgccc gccggaagcc gccggcgctc atgatggtga
    10321 cgagcgtccg gagctggctg acgtccagat ttcggtacac cgggtgtccc tccgtgtcgg
    10381 tgtgcgaggg cacgggctcg gaacgggacc gctcaccgaa cccccgccct cccggcggac
    10441 ctgaccgacc ggcaataggg agcgtggatc aaggagcggg tcgagagccg agtctgggcg
    10501 tgcatcgtgc gggggtcccc ccggccgtca ggctggggga agagagcgca gcggcgctgc
    10561 gcgcgacgag ggatgtgcgt ccgagggccc ctcgccccca gcctccggcc ggggggaccc
    10621 ccgcccgttg gcgggcaagg acggcccctc ctgcccgatg gcgggcagga gggagagaga
    10681 gctatcggca cgcgacgccg cgggccctat tgccggtcgg tcctagccca gctggccggc
    10741 ccgcggttcg acgacgtggt ccaccaaccc gtactcctgc gcctcccgtg cggtgaggat
    10801 cttgtcccgc tcgatgtcgt cgcggatctg ctggacgtcc ttgctggagt gcttggccag
    10861 catctcctcc agctgcccgc gcatccgcag gatctcgttg gcctggatct ccaggtcgga
    10921 gacctggccg cggccggtct cggtgtacgg ctggtggatc aggatccggg agttgggcag
    10981 cgccatccgc ttgccggggg tgccggccgc cagcaggacc accgcggcgg aggccgcctg
    11041 gcccatgcag accgtcgaga tgtcgggctt gacgtactgc atcgtgtcgt agaccgcggt
    11101 cagcgcggtg aacgatccgc cgggcgagtt gatgtacagc gagatgtcgc ggtccgggtc
    11161 catggactcc aggcagatga gctgggccat gatgtcgttc gcggaggtgt cgtcgatctg
    11221 cgctccgagg aagacgatcc gctcctcgaa cagcttggcg tacgggtcgt actcgcggaa
    11281 gccctgcgag gtgcgctcgg tgaagcgcgg gacgatgtag cggccctcgg cgcgcaggcg
    11341 ctcgtgggcg gcgcccatta agtcggggat gttcatgggg acttcggcct ccgtcaggag
    11401 atgggctggt ggacgggcgg tgtcgctggg tggcggtctc aggcgcccgt gccgccaccg
    11461 ccgggcacgt ccgcggcgtg gtgcatcacg tcgtcgatca ggccgtactc cttggcctgc
    11521 cgggcggaga accaacggtc gcggtcggag tccgcggtga tctcctcgac ggtccggccg
    11581 ctgtggaagg cgatcagttc ggccatccgc ttcttggtgc gcagcagttg ctcggcctgg
    11641 atccggatgt cggtggccga accgccgaga ccggccgagg gctggtgcat caggatctcg
    11701 gcgttcggca gcgcgaagcg cttccccggg ctgccggcgg tgagcaggaa ctggcccatc
    11761 gaggccgcca tgcccatcgc gatcgtcacc acgtcgttct tgacgtactg catggtgtcg
    11821 tagatcgcca tgccggccga gatcgagccg cccggggagt tgatgtagag gaagatgtcc
    11881 ttctccggct cggcggccag gagcaggagc tgggcggtga tccggttggc gatgtcgtcg
    11941 tccaccgcct cgcccaggaa gatgatgcgg tcctggagca ggcggttgta cacctgatcg
    12001 ccgaggccgc ctccggcggc ggacggagtc gtcattccca cgcgcttggc cagtgtcacg
    12061 gtgcaaccgc cttgtcagtc aggggtgttc tggtctcggg tgccgttcac ggaggtggcg
    12121 gggagcagac ggcggagggc cgatgggccg gtgcgcgatc gccgcggagc ccggcgggcc
    12181 gcagggcagg tagcggacgg catcggccgg gcggcagccg gcccggggcc cgacgcacac
    12241 cgaagcggcc cgtcggccgg ggcccggcgc cgtcggcggc ctgccgcacg gtcacgtccc
    12301 gatgacctcg ctcgcccgca ggccgcagcc cgcgctccgg atcgccgggc gcgctccccg
    12361 ccgccgtctt cgcagatcgt ggctgttcag ctgctacccc ctccggccgt cgtacccctg
    12421 tgtggctgag tgaccgtcga tcatcgccgc catcggagat gatcccgccc tgacactctc
    12481 tctctttccc cttgccagac gcaagttgat ccgtcaatta cctcataaga ccggcgggca
    12541 acagggagcg tggatgaagg agcgggtcga gagccgagtc tgggcgtgca tcgtgcgggg
    12601 gtccccccgg ccgtcaggct gggggaagag agcgcagcgg cgctgcgcgc gacgagggat
    12661 gtgcgtccag acgagagcta tcggcacgcg acgccgcgga gttgcccgcc ggtccaagcc
    12721 gcacggacaa ccggcgagtt cctcacaccg ggcagggccc gtcagcctgc acgggctgga
    12781 ccccggacca gaccatggcg gccgatcccc aggtcaggcc accgccgaag gccgtcatca
    12841 gcacccggtc gcccttcgag agccggtcgt ccgcggcggc ctgggccagg gccagcggaa
    12901 tggacgcccc ggcggtgttg cccacctccg cgagccggca gaccacccgc tcctcggcca
    12961 ggcccaggcg cctgcccacc gcgcgcagga tccgggcgtt ggcctggtgc ccgacgaacg
    13021 cgtcgatctg ctcggtccgc cagccgaccg cctcggccac gctccgcgcg gatcgcgtca
    13081 tcgccgccac cgcctgcgcg tagacctcgc gcccgtccat ccggaagtag cgcgcggcgg
    13141 agtccgcggc caggtgcggt gcccgcgacc cgccccccgg gatggcgatc aggtcggcgc
    13201 cggcgccgtc gctgcccagc tcgacgtgga gcagttcacc cggcgtgccg ggcaggccgc
    13261 gttccagcac cacggccgcc gccccgtccc cgaagagcac tgcggtcccc cggtcggcgg
    13321 ggtcgacgat ggtggagtac gtctcggcgc cgatcagcag cacgctctcg tagctgcccg
    13381 cgccgatcat cgccccggcg acgctgagcc cgtagacgaa gcccgagcac accgccgaca
    13441 ggtcgaaggc cggcacaccg gccagcccga gccgcgcggc gatctgcgga gcggtggccg
    13501 ggcagcgctg gtcgggggtg gtggtggcca gcagcaccag gtcgcagtcg cgccggccgg
    13561 ccgactccag cgccgcggca ccggcggccg tccccaggtc accggtgctc gtaccgggtc
    13621 tgaccgcacg ccgctcggcg atccccgtgc gactgaagat ccagtcgtgt gtggtgtcca
    13681 gcacccggga cagatcgtcg ttggtcacca cgtcgggcgg caggcagctc ccgataccgg
    13741 tgacgacggc tcggacgtcg gtcgtgttca t
//
