LOCUS       BGC0001468              8966 bp    DNA     linear   BCT 23-FEB-2018
DEFINITION  Streptomyces cinnamoneus strain DSM 41675
            NODE_1_length_6463510_cov_311.439, whole genome shotgun sequence.
ACCESSION   BGC0001468
VERSION     BGC0001468
KEYWORDS    .
SOURCE      Streptomyces cinnamoneus
  ORGANISM  Streptomyces cinnamoneus
            Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
            Streptomyces; Streptomyces cinnamoneus group.
COMMENT     Bacteria and source DNA available from DSMZ.
            This work was funded through Medical Research Council Newton Fund
            grant MR/P007570/1 (UK).
            Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-08 01:52:07
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(1..903)
                     /codon_start=1
                     /gene="bcmG"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) 2OG-FeII_Oxy"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003100219.1"
                     /locus_tag="CYQ11_26520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="PPT15944.1"
                     /sec_met_domain="2OG-FeII_Oxy (E-value: 9.9e-13, bitscore:
                     37.7, seeds: 135, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTAQGYGWQTAALRGGELVFSTPGGIEQALRDGFFHVEQPEGLD
                     LTAGDRFARGFYLPGEPDSTDPFRGFQHWTSERLGPRQGYYCRDDDQTEQFFLESAHWD
                     SVYPQALARQAEAMRSLALDVLRAVLAHLELPPELWDEATGRCLSARGTYNLTFNHFRP
                     EVPRRGLNVHKDSGWVTVLRSTDPGLEVERDGAWHPIDPRPGTFIVNFGCAIEILTRDT
                     RTPVAAVAHRVVQQPRTDERKPDRFSYALFVDSSLDEDICPGLFRYEPGTGLRLETNFG
                     TFLDTILHNTYQKDTAGLY"
     protocluster    1..8966
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[6125:6851]"
                     /cutoff="5000"
                     /detection_rule="(CDPS or cycdipepsynth)"
                     /neighbourhood="10000"
                     /product="CDPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      6126..6851
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="(CDPS or cycdipepsynth)"
                     /neighbourhood="10000"
                     /product="CDPS"
                     /protocluster_number="1"
     cand_cluster    1..8966
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(CDPS or cycdipepsynth)"
                     /kind="single"
                     /product="CDPS"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..8966
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0001468"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..8966
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="CDPS"
                     /region_number="1"
                     /rules="(CDPS or cycdipepsynth)"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            complement(1..903)
                     /gene="bcmG"
                     /locus_tag="CYQ11_26520"
     gene            complement(900..1790)
                     /gene="bcmF"
                     /locus_tag="CYQ11_26525"
     CDS             complement(900..1790)
                     /codon_start=1
                     /gene="bcmF"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) 2OG-FeII_Oxy"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:PF03171.18"
                     /locus_tag="CYQ11_26525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="PPT15945.1"
                     /sec_met_domain="2OG-FeII_Oxy (E-value: 6.4e-12, bitscore:
                     35.1, seeds: 135, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTVVDNEGHLHLPTARVTAGRLLFDAAEGADQALALGAFCLAVP
                     EDLDVEPGLRFCRSFYEPAEPGTADRYRGHREDGHADSKLGYEDRPDQVEQLQLESHLW
                     SRYLPEEVTALLERMKDLTLDALYGVFDVAGIPEHDRETVTGGARQDTGLCYTTVNHYR
                     ADLSDRAGIVEHSDSGFITLICTDQPGYEILHEGRWRPVREEPGHFVVNLGDAFRVLTR
                     KLPRPVTAVYHRVPELRPDGAAHHRSSFTIYMGPRYDMMLHQYAADGTLHEYQGFRDFS
                     VEKSKKLGYEFHSRI"
     gene            complement(1832..2776)
                     /gene="bcmE"
                     /locus_tag="CYQ11_26530"
     CDS             complement(1832..2776)
                     /codon_start=1
                     /gene="bcmE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) 2OG-FeII_Oxy"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003122303.1"
                     /locus_tag="CYQ11_26530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="PPT16816.1"
                     /sec_met_domain="2OG-FeII_Oxy (E-value: 2.6e-09, bitscore:
                     26.7, seeds: 135, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MASPDSATLREPVVLPPMPGEHEARAAYPPIGLERSRVTGGRLVF
                     DRDEGFDRALAQGFFLVRIPEGTDPAAGDRFAAHFHEERAGGDPLDAYRGYRHVRVPGD
                     YQGYFDREHDQWENFYVERDNWDVLPSEVARVGRGMAGLGVTILRGVLEHLRLPREHWA
                     RVTGGLTEDRGHQMLAFNHFRSHKGVRGSKFHRDSGWVTVLRSVDPGLLALVDGRLWAV
                     DPEPGHFIVNFGSSLEVLTERLDRPVRANVHGVVSTERAPGQPDRTSYVTFLDSDLTGT
                     VYRFENGTPRPLQSVAEFAGQEVGRTYDDSGAL"
     gene            complement(2776..4242)
                     /gene="bcmD"
                     /locus_tag="CYQ11_26535"
     CDS             complement(2776..4242)
                     /codon_start=1
                     /gene="bcmD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1034:cytochrome P450 (Score: 72.1; E-value: 7.2e-22)"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003100210.1"
                     /locus_tag="CYQ11_26535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="cytochrome P450"
                     /protein_id="PPT15946.1"
                     /sec_met_domain="p450 (E-value: 2.8e-13, bitscore: 38.4,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAPAHPPACPVSGRAPFPGVLAHHPGPSPLDGHESAFHEATVVR
                     GTPASEYFRASGISACAEENGGLCTFRMGPRLAVYQITNGPLLDDEDLAPSTDANRELF
                     GDFMGSLPGDHPDRPAKRAAVETTLGNGRFVEELVPHVRRHAAAFLDRAAGREVPLDEF
                     ALSLVAQVDSLVPGVLDLTQRPLPDWLASPEYGAVVRGFFDLASDVITNVNPAAMREFD
                     VIVPFVRELLRANADAIAAAPASNVIRRYFALWDLPFSREGVDGLDAAQVKELGTVIVA
                     TYDTTALSLLWALAYIETTPAAKREIVAEARGGQPSASPSPLDLAVLEAVRLGGSNPSA
                     LWRRTTRPFTLHHEGRSVTVPPGTMMWLDRRQANRDPAVFPHPEGFDPRNIRALFRSGR
                     ETVSSLISRGRHEINSFSMVNATRNPRKCPGRLFSVRVQSVLLAELYSRYEVSARGIDL
                     SLKRHAAMPRPARPGTVLFNALPERTKEQN"
     gene            complement(4239..5156)
                     /gene="bcmC"
                     /locus_tag="CYQ11_26540"
     CDS             complement(4239..5156)
                     /codon_start=1
                     /gene="bcmC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) 2OG-FeII_Oxy"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:PF03171.18"
                     /locus_tag="CYQ11_26540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="PPT15947.1"
                     /sec_met_domain="2OG-FeII_Oxy (E-value: 4.1e-14, bitscore:
                     42.1, seeds: 135, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTETLRLQKARATEEGLAFETPGGLTRALRDGCFLLAVPPGFDT
                     TPGVTLCREFFRPVEQGGESTRAYRGFRDLDGVYFDREHFQTEHVLIDGPGRERHFPPE
                     LRRMAEHMHELARHVLRTVLTELGVARELWSEVTGGAVDGRGTEWFAANHYRSERDRLG
                     CAPHKDTGFVTVLYIEEGGLEAATGGSWTPVDPVPGCFVVNFGGAFELLTSGLDRPVRA
                     LLHRVRQCAPRPESADRFSFAAFVNPPPTGDLYRVGADGTATVARSTEDFLRDFNERTW
                     GDGYADFGIAPPEPAGVAEDGVRA"
     gene            complement(5146..6123)
                     /gene="bcmB"
                     /locus_tag="CYQ11_26545"
     CDS             complement(5146..6123)
                     /codon_start=1
                     /gene="bcmB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) 2OG-FeII_Oxy"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003122306.1"
                     /locus_tag="CYQ11_26545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="isopenicillin N synthase family oxygenase"
                     /protein_id="PPT16817.1"
                     /sec_met_domain="2OG-FeII_Oxy (E-value: 4.6e-12, bitscore:
                     35.5, seeds: 135, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSRAPGNTAAPEIRRGRIYRDLYEKRASGPAVQGDAHLERARIQG
                     DRLEFAGSRARETALADGVFLLEIPADIDVAAGDAFSRQFHLGPDSPPYGRFRDLGSEH
                     FGDPLLGFHQRVNQIEQFLLERRFWASDYPPEIARLGEQLTRLSQKVLCAVLSHVGVPE
                     RDRRRATGGCSRAAGSYHLTFNHYRPEHRDVGLSSHKDDGFLTILRTTTPGLEVNRKDR
                     WERVPVDPDCFVINFGLSMEILTAPTKAPVAAIMHRVARQGGDRSSFGHFSSSGCAPGM
                     DEGVFRYLPGSGLDRVCGSRELIDENDHEIYAGTDAPGDKRREH"
     gene            complement(6126..6851)
                     /gene="bcmA"
                     /locus_tag="CYQ11_26550"
     CDS             complement(6126..6851)
                     /codon_start=1
                     /gene="bcmA"
                     /gene_functions="biosynthetic (rule-based-clusters) CDPS:
                     CDPS"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003158562.1"
                     /locus_tag="CYQ11_26550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="tRNA-dependent cyclodipeptide synthase"
                     /protein_id="PPT15948.1"
                     /sec_met_domain="CDPS (E-value: 8.3e-19, bitscore: 57.2,
                     seeds: 17, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEPREEPAKPVFGRRYKAEIGSVSPTTSRDTFEDHDTCFLGVSLE
                     NSNFKPAKVDAMAKWISRRFSQCTVLIGDSIHRITLESTRSMPPRAALDDALRLGREFV
                     ESRQPVFESFRDRTKFTFVTCSEVQSWGLYGDYHERLRQHYDQDAAFRGSVEAFGRDYH
                     GKRSEGVSAQELDHRIRKSSEYFLEEFAIFACLQRTGSPVMVYPGSFSTLSEIAQGKHP
                     GAPEELRDLIVVSLHLKGR"
     gene            7544..8965
                     /gene="bcmT"
                     /locus_tag="CYQ11_26555"
     CDS             7544..8965
                     /codon_start=1
                     /gene="bcmT"
                     /gene_functions="transport (smcogs) SMCOG1005:Drug
                     resistance transporter, EmrB/QacA (Score: 304.4; E-value:
                     1.7e-92)"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003952891.1"
                     /locus_tag="CYQ11_26555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MFS transporter"
                     /protein_id="PPT15949.1"
                     /transl_table=11
                     /translation="MLKGPPGQRRALAAVALGVFCIQLDAFALNLALPSIGHDLGASTG
                     SLQWAVSAYLLSTGTLMLGAGRLSDLWGRRRLLVVGLALFGASSLACATAPSLPLLVGA
                     RVAQGAGASLIMPVGLALLTNVYPADQRGRATGWALGMGGVATACGPFVGGALTEAASW
                     RVIFWINVPLAVAAAVWASRTPGSRDTAAAPAVDWRGLAFATAALAAVAVFVERGPVWG
                     WTSPRDGLVLAAAAVLLVAFVRHEGRVADPLVNLGLFRNGPFVVLTVAGAVANAATVLF
                     LFVVPLALQGSWGLTAFAAGVAFLAPAAMMAVAGPLAGRVTPGAAVNVMALLLALAGGM
                     LCAAPAIGALPVHLATMTVCGGVLGLANALTLIATQAVIRPERAGEASGVTKTVITVAA
                     GLGVALSGAVADPDGRPASSAAADHALTATGLAALAAGLLLGVWSAARARADRTGRTRP
                     EAGRTRRAMARTGRR"
ORIGIN
        1 tcagtacagg ccggcggtgt ccttttggta ggtgttgtgc aggatggtgt cgaggaacgt
       61 gccgaagttc gtctccaggc ggagaccggt gcccggctcg tagcggaaga ggcccgggca
      121 gatgtcctcg tccaggctgc tgtcgacgaa gagcgcgtag gagaacctgt cgggtttccg
      181 ctcgtcggtc cggggctgtt gcacgacgcg gtgcgccacg gccgccacgg gagttctcgt
      241 gtcacgggtg aggatctcga tggcgcagcc gaagttcacg atgaacgtgc ccggcctcgg
      301 gtcgatgggg tgccaggcgc cgtcacgctc gacttcgagg ccggggtcgg tggaccgcag
      361 cacggtcacc cagccggagt ccttgtggac gttcaggccg cgccggggga cttccggacg
      421 gaagtggttg aaggtgaggt tgtaggtgcc ccgtgccgac aggcagcggc ccgtggcctc
      481 gtcccacagc tccgggggca gttcgaggtg ggcgagcacg gccctgagga cgtcgagtgc
      541 cagtgaccgc atggcttccg cctgccgcgc cagcgcctgc gggtacacgg agtcccagtg
      601 ggcgctctcc aggaagaact gctcggtctg gtcgtcgtcg cggcagtagt agccctggcg
      661 ggggccgagc cgttccgagg tccagtgctg gaagccgcgg aacgggtcgg tggagtccgg
      721 ttccccgggg aggtagaagc cgcgcgcgaa gcggtccccg gccgtgaggt ccaggccctc
      781 cggttgttcg acgtggaaga agccgtccct gagggcctgc tcgatccctc ccggcgtgct
      841 gaagaccagc tctccgccgc gcagtgcggc cgtctgccag ccgtatccct gtgccgtgct
      901 catatgcggg agtggaactc gtagcccagc ttcttggact tctccaccga gaagtcgcgg
      961 aagccctggt actcgtgcaa ggtgccgtcc gcggcgtact ggtggagcat catgtcgtac
     1021 cgcggcccca tgtagatcgt gaacgatgac cggtggtgcg cggcgccgtc cggccggagt
     1081 tcgggaacgc ggtggtagac ggcggtcacc ggccggggga gcttccgcgt caggacgcgg
     1141 aacgcgtcac ccaggttcac cacgaagtgc ccgggctcct cgcggacggg ccgccagcgg
     1201 ccctcgtgga ggatctcgta gccgggctgg tcggtgcaga tgagggtgat gaacccgctg
     1261 tcggagtgct cgacgatgcc cgcccggtcg ctcaggtccg cgcggtagtg gttgaccgtg
     1321 gtgtagcaca ggccggtgtc ctggcgggcg ccgccggtga cggtctcgcg gtcgtgctcc
     1381 gggatgcccg ccacgtcgaa cacgccgtag agcgcgtcca gggtcaggtc cttcatccgc
     1441 tccaggaggg cggtgacctc ctcgggcagg taccggctcc acaggtggga ctccaactgg
     1501 agctgctcga cctggtcggg gcggtcctcg tagccgagct tggagtcggc gtgcccgtcc
     1561 tcgcggtggc cccggtaccg gtcggccgtc cccggctcgg ccggttcgta gaagctgcgg
     1621 cagaacctca gccccggctc gacgtcgagg tcctcgggaa ccgcgaggca gaacgcgccc
     1681 agcgcgaggg cctggtccgc cccctcggcc gcgtcgaaga gcagtctgcc ggccgtgacc
     1741 cgcgccgtgg ggaggtggag gtgtccttcg ttgtcgacga ccgtcgtcat cgcagtcctt
     1801 tctctggggg ggtgcaccgc ctcggaacgg ctcagagcgc accgctgtcg tcgtaggtcc
     1861 ggccgacttc ctggccggcg aactcggcca ccgactggag gggccggggc gtgccgttct
     1921 cgaaccggta gacggtgccg gtgaggtcgg agtcgaggaa ggtgacgtag gaggtccggt
     1981 ccggttgtcc cggcgcccgt tccgtggaga cgacgccgtg cacattggcc cgcaccggtc
     2041 ggtcgaggcg ttcggtcagc acttcgaggg agctgccgaa gttgacgatg aagtggccgg
     2101 gctccgggtc gacggcccac aggcgcccgt cgacgagggc gagcagaccc gggtccacgg
     2161 accgcaggac cgtcacccag ccggagtccc ggtggaactt cgagccgcgc acgcccttgt
     2221 gcgaccggaa gtggttgaag gcgagcatct ggtggccgcg gtcctcggtg agcccgcccg
     2281 tgacgcgcgc ccagtgctcc cggggcagcc gcaggtgctc caggacgccg cgcaggatcg
     2341 tgaccccgag accggccatg ccccggccca cccgggcgac ctcggatggc agcacgtccc
     2401 agttgtccct ctcgacgtag aagttctccc actggtcgtg ctcgcggtcg aagtagccct
     2461 ggtagtcgcc gggcacgcgc acgtggcggt agccgcggta ggcgtccagc gggtccccgc
     2521 cggcccgctc ctcgtggaag tgggccgcga agcggtcgcc ggcggcgggg tccgtgccct
     2581 cggggatccg tacgaggaag aacccctgcg cgagggcacg gtcgaagccc tcgtcgcggt
     2641 cgaagacgag ccggccaccg gtgacgcggg agcgctccag cccgatcggc ggatacgccg
     2701 cccgcgcctc gtgctcaccg ggcatgggag gcaggacgac cggttcccgg agggtggcgg
     2761 aatcgggtga cgccatcagt tctgctcctt cgtgcgctcg ggcagggcgt tgaaaaggac
     2821 agtgccgggc cgggcgggcc ggggcatggc ggcgtgcctc ttcagactca ggtcgatgcc
     2881 tcgcgcgctg acctcgtagc gggagtacag ctcggccagg aggaccgact gcacgcgcac
     2941 ggagaacagc cggccggggc atttgcgcgg gttccgcgtg gcgttcacca tgctgaacga
     3001 gttgatctcg tgccggcccc gggagatcag ggacgagacc gtctcgcggc cggaccggaa
     3061 cagcgcccgg atgttgcgtg ggtcgaaacc ctcggggtgc gggaagacgg ccgggtcccg
     3121 gttcgcctgg cggcggtcca gccacatcat ggtgcccggg ggaacggtga ccgaacggcc
     3181 ctcgtggtgc agggtgaagg gccgtgtcgt ccggcgccag agcgcgctgg ggttgctgcc
     3241 gcccagccgg acggcctcca ggaccgccag gtccagcggc gagggcgacg cgctcggctg
     3301 cccgccgcgc gcctcggcga cgatctcccg cttcgcggcg ggcgtcgtct cgatgtaggc
     3361 gagggcccac agcaggctga gcgcggtcgt gtcgtacgtg gccacgatca ccgtgcccag
     3421 ttccttgacc tgtgcggcgt ccagcccgtc cacgccctcc cgggagaacg gcaggtccca
     3481 cagggcgaag tagcgccgga tcacgttgga cgcgggggcc gccgcgatcg cgtccgcgtt
     3541 ggcccgcagg agttcgcgga cgaaggggac gatgacgtcg aactcccgca tggccgcggg
     3601 gttgacgttg gtgatcacgt ccgaggcgag gtcgaagaag ccccgcacca ccgcgccgta
     3661 ctccggcgag gccagccagt cgggcagggg gcgctgcgtg aggtccagca cgccgggcac
     3721 gaggctgtcc acctgggcca ccagggacag ggcgaactcg tccaggggga cctcccgccc
     3781 ggccgcccgg tcgaggaacg cggcggcgtg ccgccgcacg tgcggcacca gctcctccac
     3841 gaacctgccg ttgcccaggg tggtctccac ggccgcccgt ttggccggcc ggtccgggtg
     3901 gtcgccgggc agggagccca tgaagtcgcc gaacagctcc cggttggcgt cggtggacgg
     3961 cgccaggtcc tcgtcgtcca gcagggggcc gttggtgatc tggtacacgg ccaggcgcgg
     4021 gcccatgcgg aacgtgcaga ggccgccgtt ctcctcggcg cacgcgctga tgcccgaggc
     4081 gcggaagtac tccgaggccg gtgtcccgcg gacgacggtc gcctcgtgga aggcgctctc
     4141 gtggccgtcc agcgggctcg ggcccggatg gtgggcgagc acgccgggga agggggcccg
     4201 cccggacacg ggacaggccg gcgggtgggc gggcgcggtc atgccctcac cccgtcttcc
     4261 gcgacgccgg ccggctccgg aggggcgatc ccgaagtcgg cgtagccgtc gccccacgtc
     4321 cgctcgttga agtcgcgcag gaagtcctcg gtgctccggg ccaccgtcgc cgtgccgtcg
     4381 gcaccgacgc ggtagaggtc gccggtcggc ggtgggttga cgaaggcggc gaaggagaag
     4441 cggtcggcgg actccggccg cggcgcgcac tgccggaccc ggtgcagcag ggcccgcacg
     4501 gggcggtcca ggccggaggt gagcagttcg aaggccccgc cgaagttgac cacgaagcag
     4561 cccggtacgg gatcgaccgg cgtccaggaa ccgcccgtcg ccgcctccag tcccccctcc
     4621 tcgatgtaga ggacggtcac gaagcccgtg tccttgtggg gcgcgcaccc gagccggtcg
     4681 cgctcggagc ggtagtggtt ggcggcgaac cactccgtgc cgcggccgtc gacggccccg
     4741 ccggtgacct cgctccacag ctcccgggcc acccccagct cggtcaggac cgtgcgcagc
     4801 acgtgccggg ccagctcgtg catgtgctcg gccatgcgcc ggagctccgg cgggaagtgg
     4861 cgctcccgcc ccggtccgtc gatgaggacg tgttcggtct ggaagtgctc gcggtcgaag
     4921 tagacgccgt ccaggtcccg gaagccccgg taggcgcgcg tgctttcgcc gccctgctcc
     4981 accgggcgga agaactcgcg gcacagggtc actccgggcg tggtgtcgaa gccgggcggt
     5041 acggcgagga ggaagcagcc gtcccgcagg gcccgggtca ggccgccggg cgtctcgaac
     5101 gccaggccct cctcggtggc ccgcgccttc tggaggcgca gcgtctcagt gctcacgtcg
     5161 cttgtccccc ggggcgtcgg tgccggcgta gatctcgtgg tcgttctcgt cgatgagttc
     5221 ccgggagccg cagacgcggt cgagtccact gccggggagg tagcggaaga cgccttcgtc
     5281 catgccgggg gcgcagccgc tggagctgaa gtgcccgaag ctggaacggt cgcctccctg
     5341 ccgggcgacg cggtgcatga tggcggccac gggcgccttc gtgggcgccg tgagaatctc
     5401 catcgacaga ccgaagttga tgacgaagca gtccgggtcg acggggaccc gttcccagcg
     5461 gtccttgcgg ttgacctcca gccccggggt cgtcgtgcgc aggatcgtga ggaacccgtc
     5521 gtccttgtgg gagctcagac cgacgtcccg gtgctcgggg cggtagtggt tgaaggtcag
     5581 gtggtacgag ccggccgccc gggagcatcc gccggtggcg cgtcgccggt cccgctcggg
     5641 gacgccgacg tgggagagca ccgcgcagag gaccttctgc gacaaccggg tcagctgctc
     5701 gccgagccgg gcgatctcgg gcgggtagtc gctcgcccag aagcgccgct ccaggaggaa
     5761 ctgttcgatc tggttcaccc gctggtggaa gcccagcagg ggatccccga aatgctcgct
     5821 gccgaggtcg cggaaccttc cgtagggagg ggagtccggg ccgaggtgga actggcggga
     5881 gaaggcatcg ccggccgcga cgtcgatgtc cgcgggtatc tccaggagga agacgccgtc
     5941 ggcgagggcg gtttcccgcg cccgggagcc ggcgaactcc aggcggtcgc cctggatacg
     6001 ggcgcgttcg aggtgggcgt cgccctggac cgcgggcccg ctcgcgcgct tctcgtagag
     6061 gtcgcggtag atccggccgc gccggatttc gggtgcggcc gtgttgccgg gtgcacggga
     6121 catggtcatc gccctttcag gtggagggac accacgatga ggtcgcgcag ctcttcgggg
     6181 gctcccgggt gttttccctg agctatttcc gagagagtgc tgaacgatcc gggatacacc
     6241 atgacgggac tgccggtccg ctggaggcag gcgaagatgg cgaattcctc tagaaagtac
     6301 tcggaggatt tcctgatgcg gtggtcgagt tcctgggcgc tgacgccttc ggagcgtttg
     6361 ccgtggtagt cgcggccgaa cgcctcgacc gagccgcgga aggcggcgtc ctggtcgtag
     6421 tgctgccgca ggcgctcgtg gtagtcgccg tagaggcccc agctctgcac ctccgagcag
     6481 gtcacgaagg tgaacttggt ccggtcgcgg aaggactcga agaccggttg ccggctctcg
     6541 acgaactcgc ggccgagccg cagggcgtcg tcgagggccg cccgcggcgg catggagcgg
     6601 gtggactcca gggtgatccg gtgaatgctg tcaccgatga ggacggtgca ctgggagaac
     6661 cgccgggaga tccacttggc catggcgtcg accttggccg gtttgaagtt gctgttctcc
     6721 aggctcactc cgaggaaaca ggtgtcgtga tcctcgaacg tatcgcggct cgtggtcggg
     6781 gagacggatc ctatctccgc cttgtagcgc ctgccgaaaa ccggctttgc cggttcttcc
     6841 cgaggctcca tcagctgcgc ttctagcgac ataaagccaa cccgcctagt cgtgggggag
     6901 ggtggggtca atggccgaat ggctgaccga atatgcagga tgaaattgct aacaagagcc
     6961 cggatggctg ggcaagcccg aggtttgttt tgagccggac cggaccggat ggcatcgctg
     7021 catgacctgc ggagtcggcc gctccgcaac ttagatgtag gccgaaacta cttggtcggt
     7081 gaggtgagga gcggtaggtg gaagcaagag ggattgtcgg gggagaaccg atgtcagacg
     7141 gattgtgcgg tcaatccgga cacgggggag agtaaagccg ccgtagggga tgcgggcgca
     7201 ccggatccgc ccggaagggg gtggcccgag cgctgcggac cggctaacgt gaggagcgct
     7261 tcgtgccgca ccccgcacca aggcgacccg tttctccacg gcgccccggg gagcgcggat
     7321 ctggagccgt tgcggcgccc ccggttcaca tccggcggcc actcacgtca ggttctgaca
     7381 cctcatcccc tcaccgcctg cccgaaccgg ccgggcagca cgcgccgttg cccgcggacg
     7441 accgggccac agcggccccg gtgccgtacg gctcgtaccg cgcgccccgt gcccggcccc
     7501 cgtgacgacg tggctcccgc cacccgaagg atgtgagacg actgtgctga agggacctcc
     7561 tggacagcgc cgggccctgg cggccgtggc gctgggggtg ttctgcatcc aactggacgc
     7621 cttcgccctg aacctggccc tgccgagcat cggtcacgac ctcggcgcct cgaccggcag
     7681 cctgcagtgg gccgtcagcg cgtacctgct gtccaccggc acgctcatgc tcggcgccgg
     7741 ccgcctgagc gacctctggg gacgccgccg gctgctggtc gtcgggctgg ccctgttcgg
     7801 cgcctcctcc ttggcctgtg ccaccgcccc gtcgctgccg ttgctcgtcg gcgcgcgggt
     7861 ggcgcagggc gccggcgcct ccctgatcat gccggtcggc ctggccctgc tgaccaacgt
     7921 ctacccggct gaccagcgcg gccgtgccac gggatgggcc ctcggcatgg gaggcgtcgc
     7981 caccgcctgc ggcccgttcg tcggcggggc gctgaccgag gcggcgtcct ggcgcgtcat
     8041 cttctggatc aacgtcccgc tggccgtcgc cgcggcggtg tgggcctcgc ggacgcccgg
     8101 gagccgggac accgccgccg ccccggccgt cgactggcgg gggctggcct tcgccacggc
     8161 ggccctcgcg gccgtcgccg tgttcgtgga acgcggcccg gtgtggggct ggacatcccc
     8221 ccgcgacggc ctcgtgctgg ccgcggccgc cgtgctgctc gtcgccttcg tgcgacacga
     8281 gggccgggtc gccgaccccc tggtgaacct gggcctgttc cgcaacggcc ccttcgtcgt
     8341 gctcaccgtc gccggagccg tggcgaacgc ggcgaccgtg ctgttcctgt tcgtggtccc
     8401 cctcgccctg caggggtcct ggggcctcac ggcgttcgcg gcgggcgtcg ccttcctggc
     8461 cccggccgcg atgatggccg tcgccggtcc gctggccggc cgggtgactc ccggcgcggc
     8521 ggtgaacgtc atggcgctgc tgctggccct ggccggaggc atgctctgcg cggccccggc
     8581 catcggggcc ctgcccgtcc acctcgccac gatgaccgtg tgcggcggtg tgctcggcct
     8641 ggccaacgcc ctgaccctca tcgccaccca ggcggtcatc cggcccgaac gggccgggga
     8701 ggcctcgggg gtgacgaaga cggtcatcac cgttgccgcc gggctcggcg tcgcgctgtc
     8761 cggtgcagtc gccgaccccg acggcaggcc tgcgtcgtca gcggcggcgg accacgccct
     8821 caccgcgacg ggactggccg cgctcgcggc ggggcttctc ctgggcgtgt ggagcgcggc
     8881 ccgcgcgcgg gcggaccgca ccggccggac gcggccggag gcggggagga cgcggagggc
     8941 gatggcccgg acgggccgga ggtagg
//
