LOCUS       BGC0001486             11214 bp    DNA     linear   BCT 28-APR-2011
DEFINITION  Pseudomonas syringae pv. maculicola str. ES4326 contig13.14, whole
            genome shotgun sequence.
ACCESSION   BGC0001486
VERSION     BGC0001486
KEYWORDS    .
SOURCE      Pseudomonas syringae pv. maculicola str. ES4326
  ORGANISM  Pseudomonas syringae pv. maculicola str. ES4326
            Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
COMMENT     DNA Source: Jeff Dangl (University of North Carolina at Chapel
            Hill).
            Annotation was added by the NCBI Prokaryotic Genomes Automatic
            Annotation Pipeline Group. Information about the Pipeline can be
            found here:
            http://www.ncbi.nlm.nih.gov/genomes/static/Pipeline.html. Please be
            aware that the annotation is done automatically with little or no
            manual curation.
            Annotation was added to the contigs in April 2011.
            This WGS project has been superceded by the complete genome in
            (non-WGS) GenBank records CP047260, CP047261, CP047262, CP047263,
            CP047264.
            ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-08 01:59:07
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 11214
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..1407
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1180:Decarboxylase, pyridoxal-dependent (Score: 357.8;
                     E-value: 1.4e-108)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="PMA4326_22624"
                     /note="COG0076 Glutamate decarboxylase and related
                     PLP-dependent proteins"
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="EGH61603.1"
                     /transl_table=11
                     /translation="MSCILDDERCIPELLTQLRSLSLDFLSGAHTGAAVDTRSDVLTQQ
                     AEMPEEGLGCLDALRTYWQRYADGHSRSTGPRYYGFVTGGVTPAALAGDWLVSVLDQNV
                     ATERHSIAAFIEAQVLTFISNLLKLPAGLFQGVLTTGATAANLVGLSSAREWCGEQAGV
                     SIAHEGIAAAPPIAVFGASAHSSIIKSLGVLGIGRKNIVSIRSLPDREAMDIQHLEEAL
                     KSSVSTSRIVVASAGTVNSGDFDDLNGIADLCMRYNAWLHVDAAFGAFARCSPRYEHLI
                     EGLERADSITADAHKWLNVPYDCGIALTRHTAFHERSFSADAPYVPMGGELPAFMNRGV
                     EQSRRYRALPLWMTLMAYGKSGYRELVERNCDVVRQIASWIDGCEYYDVLCPVYLNIVM
                     FRCRRVGGRDHDAHSVAVLERINATGALFLTPSTYNGEKVFRLAVCNWMTTRSDVAIVI
                     NALHHAVAGY"
     protocluster    1..11214
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[4410:9956]"
                     /cutoff="10000"
                     /detection_rule="minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE])"
                     /neighbourhood="10000"
                     /product="RRE-containing"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      4411..9956
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE])"
                     /neighbourhood="10000"
                     /product="RRE-containing"
                     /protocluster_number="1"
     cand_cluster    1..11214
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE])"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="interleaved"
                     /product="RRE-containing"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /protoclusters="2"
                     /tool="antismash"
     subregion       1..11214
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0001486"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..11214
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RRE-containing"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="minimum(1, [Bottromycin_Methyltransferase_RRE,
                     Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE])"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            1..1407
                     /locus_tag="PMA4326_22624"
     protocluster    792..11214
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[5791:6706]"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="2"
                     /tool="antismash"
     proto_core      5792..6706
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="2"
     gene            1445..2656
                     /locus_tag="PMA4326_22629"
     CDS             1445..2656
                     /codon_start=1
                     /locus_tag="PMA4326_22629"
                     /note="COG0477 Permeases of the major facilitator
                     superfamily"
                     /product="major facilitator family transporter"
                     /protein_id="EGH61604.1"
                     /transl_table=11
                     /translation="MSSQVPENTPSTKGLFGLFCLASYLLSLSYGTTFLLALMLGSHGG
                     SESDAGTVISVAMLSTFLAVIFSGHLTDIIGAPRAIAAGGLTLMIACIGFAMTPTYGPA
                     LLIFGLILGFGWGVFYTLGPIIVAMIIEPARRVKYFALLSGSMMSGIGTGPLAGRGAVA
                     MGYPVESAFVVAAAASLLGAVVFFLIAPKIRLQQAVLGPVAVCRITTGAAARVLRSKAF
                     FPIVMVGLGGAIFGGLSSFQTSYAALHHLDYSLFFLGFMCAAISCRLLIAGFIVKRDAY
                     LSSCVLTSLMIVSILMFVFSVESPFSYLLAAIILGVGYGLTYSVINGLAANEAPNGLTA
                     QSLLLFSLSYFVGVFGFPLLAGKIIVAYGMPSLLYTILSVALLNGCIALGRLAWRKVAV
                     AAIA"
     gene            2776..3483
                     /locus_tag="PMA4326_22634"
     CDS             2776..3483
                     /codon_start=1
                     /locus_tag="PMA4326_22634"
                     /note="COG0500 SAM-dependent methyltransferases"
                     /product="Methyltransferase type 12"
                     /protein_id="EGH61605.1"
                     /transl_table=11
                     /translation="MVGDGLASNNAAWSFGGDTPKHFDSHVSKSVPKYKDGHDIVLSLS
                     DFFIKADSVCYELGSSTGTLTRQLAERHGRSGKWVGIDVEEAMTQQANQSLGCAPISNV
                     TFVTDDILTFPYEKSDFIVAYYTVQFVHPRVRQELFNRIYESLNWGGAFVLFEKVRGPD
                     ARFQDVISSLYVDYKMQQGYTPSEILAKTRSLKGVLEPFSTAGNQDMLARAGFKDVMSI
                     FKYICFEGFFCIK"
     gene            3497..4414
                     /locus_tag="PMA4326_22639"
     CDS             3497..4414
                     /codon_start=1
                     /locus_tag="PMA4326_22639"
                     /note="COG0500 SAM-dependent methyltransferases"
                     /product="hypothetical protein"
                     /protein_id="EGH61606.1"
                     /transl_table=11
                     /translation="MNDWAISHYLDVLHMDSGYQAVALDCELCGSEHHEVLLPRICGPE
                     PHGVDLPVLGCQACGHIFQKYRFDAQFYKDYYDKFYRLKLFGNSAPEKAFFLDQIRRGE
                     YLRQSLREYLPESGRLLDVGCSAGGLMIPFAKRGWSVTGNDPDRAYVEYGKQIGLNIEL
                     TAAEEMDAAGDVDLIIINGSLEHMYDVHCVMQRCRQASSENGLLLIEGRALGHGLEQGM
                     LTHNHRRYLSGTSIELLMRMHGWEPLLITEQPLCGPTRPGAVFVLGRCAAPSDDVQLER
                     VKAQGRERLDSYYRERFRALVLPS"
     gene            4411..5553
                     /locus_tag="PMA4326_22644"
     CDS             4411..5553
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RRE-containing: Thiaglutamate_G_RRE"
                     /gene_kind="biosynthetic"
                     /locus_tag="PMA4326_22644"
                     /note="COG0750 Predicted membrane-associated Zn-dependent
                     proteases 1"
                     /product="peptidase M50"
                     /protein_id="EGH61607.1"
                     /sec_met_domain="Thiaglutamate_G_RRE (E-value: 1.8e-53,
                     bitscore: 168.1, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHASDVSEVMKQTSIASCLRNDIVVSERVEADGRLRYKLYDPSN
                     DIGFELGKTEYALARLFDGVSTHEEISRYAETRHRIKVSQTKLVQFESRLLSLNILSGV
                     DGDLEKLRDPATGITYGPLKKYLLINLVKMNPQPGLDYVYPSVRWICSVPFCLAMLAAC
                     LVSYVYLGMHFGAFQEDVLAVYVHSNQWLWWHFPVILSSIALHEFGHALACRHYKVQVS
                     DFGIGIYLLLATGWVRPVQEQWSSLPRYQRIVTILAGPLVSLVYATAGIVIWAAYGEAQ
                     GNLLALSIVMIVASFISLIPTLLPMFNGDTYLAITELINEPRLRQRSFNYLKARLRGAP
                     CETSVRLQRLYLSTVSITCLGWVLVWGFVLQTLFRVFHNQ"
     gene            5564..5716
                     /locus_tag="PMA4326_22649"
     CDS             5564..5716
                     /codon_start=1
                     /locus_tag="PMA4326_22649"
                     /product="hypothetical protein"
                     /protein_id="EGH61608.1"
                     /transl_table=11
                     /translation="MGQPNVQSVENQQASGDVKDLENTPQATEEALFEEFDLDDIEVIE
                     SKVFA"
     gene            5792..6706
                     /locus_tag="PMA4326_22654"
     CDS             5792..6706
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /locus_tag="PMA4326_22654"
                     /note="COG3220 Uncharacterized protein conserved in
                     bacteria"
                     /product="hypothetical protein"
                     /protein_id="EGH61609.1"
                     /sec_met_domain="DUF692 (E-value: 1.9e-41, bitscore: 131.3,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPDFVKPAPIGVGIQYNPEILDWFPFEDIQVDILEILLDNIMAPM
                     DGPQIIKPSAQAMIERLGQKFTLLAHSNYGCDFGFSALEETAAVQRHVPLAKMLNSPWV
                     ANHCFYGDQSWLDIWSSPIQFSAAEVARCADRAQSLQTLYGMPLAHENAAYYLECPGAE
                     MREAEFLARLVQRSGTFLHLDLHNIYTNHLNLKGFDLKDYMDTLPLDKVISVHLAGGSW
                     HGGLYHDWHDACVPEPVWDLYEDLLSRAQPSAVILEYQGQAHHAQTRIMDASDESMIVR
                     DVQRAQAIWSRYNRHPQERQYGS"
     gene            6696..7505
                     /locus_tag="PMA4326_22659"
     CDS             6696..7505
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RRE-containing: Thiaglutamate_I_RRE"
                     /gene_kind="biosynthetic"
                     /locus_tag="PMA4326_22659"
                     /product="hypothetical protein"
                     /protein_id="EGH61610.1"
                     /sec_met_domain="Thiaglutamate_I_RRE (E-value: 6.8e-54,
                     bitscore: 169.7, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEAEQVWKLWRRVLRDERLQAQLFSATDATHWLSGFSESESKILS
                     VYAQQFDRVKWFVENYQFRLVNSFLNALETGAPLSLRALLHINVDLNAQSKAFLRDRQW
                     RDYGPQVYTYCEDVLGFLAEADELQGYPEILDLMRLERESVRLYRGLVDPESLPADNRY
                     QRTSMARLYETRFALSGWLRQKDQLGLTRLPESTEHVLIYLPTLQARHKFTLINAQAAR
                     LYNCLEQPQSAAGLFMLINSDSASVPGSADLALLDRLEQLNAIRKPL"
     gene            7509..9956
                     /locus_tag="PMA4326_22664"
     CDS             7509..9956
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RRE-containing: Thiaglutamate_B_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Lant_dehydr_N"
                     /gene_kind="biosynthetic"
                     /locus_tag="PMA4326_22664"
                     /product="Lantibiotic dehydratase domain protein"
                     /protein_id="EGH61611.1"
                     /sec_met_domain="Lant_dehydr_N (E-value: 1.3e-26, bitscore:
                     82.2, seeds: 73, tool: rule-based-clusters)"
                     /sec_met_domain="Thiaglutamate_B_RRE (E-value: 1.9e-48,
                     bitscore: 152.3, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MESSHYFWLRSTGFAVHHLTRLGKMAELPLLKDFETDYRSLNTLR
                     DSLLEKSISHSSQACRKLIRKLNENLPLQVSDLPEALRDDAVDELAHWNERLGRLKQRV
                     EVDQEYAVFLESARQALIDFVNDEDVEQAVFISNPTALTRLRELRQERHARTDSRKKQK
                     LRLAWSYAQRFCSKNDTSSFFGPLAWGRFDRTQVEHVRITQHGPGWIRERHTFFESWVV
                     QRLVEQLNRHCVDVQFMPLQLNPGCFLNQDTLHLPVNKRRQVSALTARVLGYIQCQSAV
                     APTLFGLQAELQDVSAGQLRDLIDHLVAQQIVRRGWQISPRERKPVQVLHTFLADARLS
                     DDFRTQWHERLSGLEAIRQDYATGDLAVRIASLDKLNRLLGEAGVDISRESGAMYVGRY
                     PVYEDCSRNIDISLGGALLDQVNADLAPLMRIHQWLIKACARQLHGFYAQVWQRLQAED
                     EHNPVNFLAFLGAVQPVIAHAEAGIIEELDAVLADAWQQVLSDKHDPEQVQLTHEDIER
                     LIVELNTRLDVRTFSVFGSHFHSPDFLISSTSTDALNQGDYSIILGEVHPGVHTLSQPV
                     AEPFGPFNKEIEQEVRQIFDGPRLVLADSPDSYQRSHIDWPLLECYQQLILPSGGGCVP
                     ADQRFAVGRARLVMNEGRLRVEDIAGQFSEDLVCVYPTPMHRLGFALAGSVVAKNDRRR
                     IGLGKTLYKRASWWFSPEQLPCSEFSVDKLDDVLAWRAWAVEHGLPRYVFAKIDIEPKP
                     IFIDFDNPLSLDGVSNSMKKAGHVKFSEMCPAPDQLWLEETRGHFCCEIRTTFRDNGVT
                     RDE"
     gene            9949..11214
                     /locus_tag="PMA4326_22669"
     CDS             9949..11214
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1106:major
                     facilitator transporter (Score: 144; E-value: 8.6e-44)"
                     /gene_kind="transport"
                     /locus_tag="PMA4326_22669"
                     /note="COG0477 Permeases of the major facilitator
                     superfamily"
                     /product="hypothetical protein"
                     /protein_id="EGH61612.1"
                     /transl_table=11
                     /translation="MNNSSAMDGSQQKLKSSFFLLFLTIFIPFGLGHFVSYLFRTVNAV
                     IYVDLQTDLSLPASSLGLLTGVYFLTFAAAQIPLGVMLDRYGPRSVQAPMLLFAVAGSV
                     IFSVSSSEAGLLIGRGLIGLGVAGSLMSAIKACAIWLPVERLPLSTACLLSIGGLGAMA
                     STTPLHALLSWLTWREAFLVLALLTFCVAVVIHFSVPKTYQSKNTRYGDMFAAVGKLYS
                     SWTFWRLALYSVFAHAIYMSVLSLWMGPWLRDMAGLSDSAMASVLLFGAIAIVAGSLTF
                     GAITDYLRRFGVQPIMICGTGMLIFIGFQVLMVSDLPVSPYLIAMGFSFFGTSTTMNYA
                     IVAQSVSPELAGRVSSSFNLVVFVLVFCLQWLMGVVLNLWPVAQGAGHPAEAYQWSLGL
                     MIALQVPGLLLWLSFRPWQRKV"
ORIGIN
        1 atgtcgtgca ttttggatga tgagcgctgt atccccgagt tgttgacgca gcttcgcagt
       61 ttgtcgctcg actttctgtc tggcgcacac accggcgctg cggtcgacac gcgctctgat
      121 gtcctgactc agcaagcgga aatgcccgag gaggggctcg gttgcctgga tgctctgagg
      181 acttattggc agcgctacgc tgatgggcac agtcgcagca caggccctcg gtactatggc
      241 ttcgtgacag gcggggtgac gcccgctgca cttgccggag actggctggt ttccgtcctt
      301 gaccagaacg tcgccactga gcgccattcg atcgctgcat tcatcgaagc tcaagtgctg
      361 acgttcatca gtaacctgct gaagcttccg gcgggtttgt ttcagggtgt tttgacgacc
      421 ggagcgactg cggcaaatct ggtggggctg tccagcgctc gtgagtggtg cggtgagcag
      481 gcgggagtca gcattgctca cgaaggaatt gcagcagccc ctccgatagc ggtattcggg
      541 gcgtctgccc actcaagtat tatcaaaagt ctgggtgtgc tgggcatcgg gcgcaaaaat
      601 atagtgtcaa tacgcagttt gcctgatcgt gaagccatgg atattcaaca cctggaagaa
      661 gcgctgaaaa gcagtgtttc tacttctcga attgtcgtgg ccagcgccgg taccgtgaat
      721 agcggtgatt tcgacgacct caatggtatt gctgatttat gcatgaggta caacgcgtgg
      781 ttgcatgtcg atgcggcatt cggtgccttt gctcgttgtt ctcctcgtta cgaacacttg
      841 atcgaggggc tggagcgtgc tgattcgata acggcggatg ctcacaagtg gcttaacgtt
      901 ccctatgact gtggaattgc cctgacccga catacggcgt ttcatgagcg gagcttttcc
      961 gctgacgcgc cctatgtgcc catggggggc gagcttcctg ctttcatgaa ccgaggtgtt
     1021 gaacagtcgc gtcgctatcg tgcgttgccg ttatggatga cgcttatggc ttacggcaag
     1081 tcgggttacc gcgagttggt agagcgtaat tgtgatgttg tacgccagat cgcttcgtgg
     1141 attgacgggt gtgaatacta cgacgtgttg tgccctgtct atttgaacat cgtgatgttt
     1201 cgctgccgac gtgtcggcgg gcgtgatcat gacgcgcaca gcgtggcagt gctggaaaga
     1261 atcaatgcca caggtgcgct gtttcttacg cccagtacgt acaacgggga aaaggtattt
     1321 cgtctggctg tctgtaactg gatgaccaca cgcagtgatg tcgcaattgt gatcaatgcc
     1381 ttacaccacg cggtggctgg ttattgagag cagcactgtt atcaaacgat aaggaatgtc
     1441 atacatgtct tcgcaagtgc ctgaaaatac cccttcgacc aaggggctgt tcggcctgtt
     1501 ttgcctggcc agctatctgc tgtccctgtc ttacggcacc acttttctac tggccctgat
     1561 gctgggctct catggcggaa gcgagtctga cgcaggcaca gtgatttcag tggccatgct
     1621 gagcacattt ctcgccgtca ttttttcagg gcacctgacc gatatcatcg gtgcgccccg
     1681 ggctatcgct gccggaggtc tgacgttgat gattgcatgc atcgggtttg ccatgacccc
     1741 gacgtatggc ccggcattat tgatattcgg tctgatactg ggtttcggtt ggggtgtgtt
     1801 ctacacactg gggccgatca ttgttgcaat gatcatcgag cctgcgcgtc gggtgaagta
     1861 tttcgctttg ctgtccggca gcatgatgag tgggatcggt accgggcctt tggcagggcg
     1921 cggtgcagtg gcaatggggt atccggttga atcggcgttc gtcgtcgcgg ccgctgccag
     1981 cctgctgggt gcagtcgtgt ttttcctgat agctccgaaa atcaggctgc aacaggctgt
     2041 tctcgggcct gtggccgtgt gccgcatcac tacaggtgct gccgctcgtg tactgcgctc
     2101 caaggcgttc tttcctatcg tgatggtggg attgggcggc gcaatcttcg gcgggctttc
     2161 cagctttcag acctcctacg ccgcattgca tcatctggac tattcgctgt ttttcctcgg
     2221 attcatgtgt gcggccatct cttgccgatt gttgattgcc gggttcatcg tcaagcgtga
     2281 tgcttacctg tcttcctgcg tactgacatc cctgatgatt gtttcgatac tgatgtttgt
     2341 gttctcggtt gagtcgccat tcagttattt gttggccgcg atcattcttg gcgtgggtta
     2401 tggcttgacg tattcggtga tcaacggctt ggcggctaat gaagcgccta acggactcac
     2461 cgcacagtcc ttgttgttat tcagtttgtc ttactttgta ggcgtgttcg gttttccact
     2521 gttggccgga aaaataatcg tggcttacgg catgccctca ctgctgtata cgattctctc
     2581 ggtagccctt ttaaacgggt gtattgcgtt gggccgtctt gcctggcgca aggtggctgt
     2641 cgccgccata gcctgaccgt aaacgtttca gtcaaactgc atcgcgtgac gcgctgcagt
     2701 tttcggtgtt cctgtgcaag gtaagcaagg agattcattg tgcctgttaa tgcgcctgaa
     2761 gtaataagtc ttgaagtggt cggggatgga ctggcttcaa ataacgcagc ctggagtttt
     2821 ggcggtgata cgcccaaaca tttcgactcg catgtttcga aatcggttcc caagtacaag
     2881 gacggtcacg atatcgttct ttccttgagc gacttcttta tcaaggctga cagcgtctgt
     2941 tatgaacttg gaagttcgac cggcacattg acacggcaat tggcagaacg tcacgggcgt
     3001 tcaggcaaat gggtgggtat cgatgtcgaa gaggccatga ctcaacaagc caatcaatca
     3061 ctcggctgtg cgcccatcag taacgtcacc tttgtgaccg acgacatcct tacctttccg
     3121 tatgagaaga gcgatttcat cgtcgcgtat tatacggttc agtttgtcca tccccgcgtt
     3181 cgccaggagc tgttcaatcg tatttacgag tctctgaact gggggggtgc atttgtgctg
     3241 ttcgagaaag tcagggggcc ggatgctcgg tttcaggatg tgatcagcag tttgtatgtc
     3301 gactacaaga tgcagcaggg ctatacgccg agcgagatcc tggccaagac gcgtagtctt
     3361 aaaggtgtgc tggaaccgtt ctccacggcc ggcaatcagg acatgctcgc ccgagccggt
     3421 ttcaaggacg tgatgagcat tttcaaatac atttgttttg aaggtttctt ctgcatcaag
     3481 taacagggac gttgcgatga acgattgggc tatcagccat tacctggatg tgttgcacat
     3541 ggacagcggt tatcaagccg ttgcactgga ctgcgaactc tgcggcagtg aacaccatga
     3601 ggtgttgctg ccacgtatct gcggtcctga accccacggc gttgatttgc ctgtgttggg
     3661 gtgtcaggcg tgcggccata tttttcaaaa atatcgtttt gatgcgcagt tttacaaaga
     3721 ctactacgac aagttttatc gcttgaaact gttcggcaac agcgcgccgg aaaaagcgtt
     3781 ttttctcgac cagattcgtc gtggagaata cctgcgtcag agcctgcgcg aatacctgcc
     3841 agagagtggt cgcctgctgg atgtcggatg ttcagccggt ggcctgatga tcccttttgc
     3901 caagaggggc tggtctgtca ccggcaatga ccctgatcgc gcttatgtcg aatacggaaa
     3961 acagatcggc ctgaatatcg agctgacagc ggccgaagaa atggacgccg caggcgatgt
     4021 cgatctgatt atcatcaatg gctcgctgga acatatgtac gacgtgcatt gcgtcatgca
     4081 gcgctgtcgg caggcgtcgt cggaaaacgg tctgttattg attgaaggcc gcgccctggg
     4141 tcacggcctg gagcagggca tgctgaccca caatcatcgg cgttatctca gcggcaccag
     4201 catcgaactg ctgatgcgca tgcacggctg ggagccgctg ctgatcaccg agcaaccgtt
     4261 atgcgggcct actcgacctg gcgcggtttt cgtccttggt cgatgcgcag ccccgtcgga
     4321 tgatgtgcag ctcgaacgtg tcaaggcgca agggcgtgag cgcctggaca gttactaccg
     4381 ggagcgcttc cgggcgctgg tgttgccatc atgacacacg cttccgatgt gtccgaggtt
     4441 atgaaacaga cttcaattgc gtcctgtctg cgcaacgata ttgttgtcag cgagcgtgtg
     4501 gaggcagacg gccggttgcg ctacaagctt tacgacccca gtaacgatat cggttttgag
     4561 ttgggtaaaa ccgagtatgc actggcccgc ctttttgatg gtgtatccac acacgaagag
     4621 atctcccgat acgctgaaac ccggcaccgc atcaaggtca gtcagacgaa actggtgcag
     4681 tttgaaagtc gcctattgag tctgaatatt ctgtcggggg tagatggcga tcttgaaaaa
     4741 cttcgtgatc ccgcgaccgg tattacctat gggcctttga agaaatacct gctgatcaac
     4801 ctggtcaaga tgaatccgca gccgggcctg gactatgttt accccagcgt gcgctggata
     4861 tgcagcgtac cgttttgtct ggcgatgctt gcggcgtgtt tggtcagcta cgtctacctg
     4921 gggatgcact tcggcgcatt tcaagaagat gttctcgcgg tgtatgtgca cagcaatcaa
     4981 tggctgtggt ggcattttcc ggtcatcctc agttctatcg cactgcacga gttcgggcat
     5041 gccctggcct gtcgacatta caaggttcag gtcagtgatt tcgggatcgg catttatctg
     5101 ttgctggcca ccgggtgggt gcggccggtt caggaacaat ggagttcgtt gccgaggtat
     5161 cagcgcatcg tcaccatttt ggccgggcca ctggtcagcc ttgtttacgc cactgcaggc
     5221 attgtgatct gggctgctta cggtgaagcg caaggtaact tgcttgcgct gtcgattgtg
     5281 atgattgtgg catcgttcat ctctctgatt ccaaccttgt tgccaatgtt caatggcgac
     5341 acttacctgg ccatcaccga actcatcaac gagccccggt taaggcagcg ctctttcaac
     5401 tatttgaaag cacgcttgcg cggggcacct tgtgaaacgt cagtccggct ccaacgcctt
     5461 tatctgagca ctgtatcgat cacctgtctt ggctgggtgt tggtgtgggg cttcgtgctg
     5521 cagacgcttt ttcgtgtatt tcataaccag tgaggattta cccatgggac aacccaacgt
     5581 gcagtccgtt gaaaaccagc aagcgtccgg cgatgtaaaa gatctcgaaa acactccgca
     5641 ggccacagag gaggcgttgt ttgaagagtt tgacctggat gacattgaag tcatcgagag
     5701 caaggtcttt gcctgagtgc gccatgccct cttgcgtgcc ggattgtagg gatacgcagg
     5761 agggcttctt tcatcagtgc gagtgttagc catgcctgat ttcgtcaagc ccgcgcctat
     5821 cggagtgggt atccagtaca accccgaaat actggactgg tttccattcg aggatatcca
     5881 ggtggatatt ctggagattt tgctcgataa catcatggcg cccatggatg gcccgcaaat
     5941 tatcaagcct tctgcccagg caatgattga gcggctaggg caaaaattta ccttgctggc
     6001 tcactccaat tacggctgcg attttggttt cagtgccctc gaagaaacag cggcggtgca
     6061 gcgccatgta cctctggcaa aaatgctcaa cagcccttgg gtggccaacc attgtttcta
     6121 tggggatcag tcctggctgg atatctggag cagtcccatt cagttctccg cagccgaagt
     6181 ggcgcgttgt gccgatcgcg cacagtcgtt gcagacgctt tatggcatgc cgctggctca
     6241 tgaaaatgcc gcctactacc ttgaatgccc aggcgccgaa atgcgtgagg ccgagtttct
     6301 ggcaagactg gtgcagcgct cagggacttt tcttcacctt gacctgcaca acatttacac
     6361 caaccacctc aatctgaaag gtttcgatct caaggactac atggacacgc tgccgctgga
     6421 caaggtgatt tcggtgcatc tggccggagg aagctggcac ggcggcctgt atcacgactg
     6481 gcatgacgcc tgcgtgcccg agccggtctg ggacttgtac gaagacctgc tttcgcgtgc
     6541 acagccctct gctgtgattc ttgagtatca ggggcaggcg catcatgccc agactaggat
     6601 catggatgcg tccgacgagt cgatgattgt cagggatgtc cagcgtgctc aggcaatctg
     6661 gagtcgttac aacaggcatc cgcaggagcg tcagtatgga agctgaacag gtatggaaac
     6721 tgtggcggcg cgttctgcgc gatgagcgcc ttcaggcgca gttgttttcg gctaccgatg
     6781 ccacgcactg gttgtcaggt ttcagcgagt ccgaaagcaa gatcctctcg gtgtatgcgc
     6841 agcagtttga ccgcgtcaaa tggttcgtcg agaactatca gttccggctg gtgaactctt
     6901 ttttgaacgc gctggagacc ggcgcgccct tgtcgttgcg ggcgttgctg cacataaatg
     6961 tggacctgaa tgcgcaatcg aaggccttcc tgcgtgacag gcagtggcgc gattacgggc
     7021 cgcaggtcta tacctattgt gaagatgtac tgggtttcct tgccgaggcg gatgagctgc
     7081 agggttatcc tgaaatcctt gatctgatgc ggctggagcg cgaatcggtt cgcctgtatc
     7141 gggggttggt cgatcctgag tccttgccgg cagacaaccg ttatcagcgc acctcaatgg
     7201 ctcgtcttta cgagacacgt tttgccctgt ctggctggct cagacagaag gatcagttgg
     7261 gcctgacacg tctgcccgag agcaccgagc atgtgctgat ctaccttccc acgcttcagg
     7321 ctcgtcacaa gttcactttg atcaacgcgc aggctgcccg gctttacaac tgcctggagc
     7381 aacctcagtc cgctgccggg ctgttcatgc tgatcaattc ggactcggcg tccgtgccgg
     7441 gtagcgcaga cctggcgctg ctcgacaggc tggaacagct taacgcgata aggaagcctt
     7501 tgtagcctat ggaaagctca cactatttct ggcttcgctc gaccgggttt gcggtgcatc
     7561 acctgacccg cttggggaaa atggccgaat tgccgttgct gaaggatttt gaaaccgatt
     7621 atcgctccct gaatactcta cgcgacagcc tgttagagaa gtccataagc cactcgtctc
     7681 aggcctgtcg caagctgatc cgcaagctga atgaaaacct gccgctgcaa gtgtctgatc
     7741 ttccagaagc gctgcgtgat gacgcagtgg atgaattggc gcactggaac gagcggcttg
     7801 gccggctcaa gcaacgtgtc gaagtggatc aggaatatgc cgtgtttctc gaaagcgcgc
     7861 ggcaggccct gattgatttc gtgaacgatg aagacgttga gcaggccgtg ttcatttcca
     7921 acccgaccgc gctgacccgg cttcgggagc tcaggcaaga gcgccatgcg agaactgaca
     7981 gccgcaagaa gcagaaactg cgcctggcct ggagctacgc acagcgattc tgttcaaaaa
     8041 atgatacgtc gagttttttc ggcccgctgg cctgggggcg gtttgatcgt acgcaggtcg
     8101 agcatgttcg tatcacgcag cacggacccg gctggatcag agaacgccat acctttttcg
     8161 agagctgggt tgtacagcgg ctggtcgagc agttgaatcg ccattgcgtt gatgtgcagt
     8221 tcatgccgtt gcaactcaac ccgggctgtt ttttgaatca agacacgttg catctgcccg
     8281 tcaacaaacg tcgtcaagtc agtgccttga cggccagagt gctgggttac attcagtgcc
     8341 agagcgctgt ggcaccgacc ctgttcgggt tgcaggccga gttgcaggat gtcagcgccg
     8401 ggcaattgcg cgacctgatc gatcatctgg tcgcccagca gattgtacgt cgcggctggc
     8461 agatttcccc cagggaacga aagcctgttc aggtgctgca cacttttctg gccgacgcgc
     8521 gcctgtctga tgactttcgc actcagtggc atgaacgtct gtcgggcctt gaagcgattc
     8581 ggcaagacta tgcgacaggc gatctggccg tacgcattgc cagcctcgac aagctgaacc
     8641 ggctgcttgg cgaagccggc gtggatattt cgcgtgagag cggcgccatg tatgtcggtc
     8701 gctacccggt ctacgaggat tgttcgcgca atatcgatat cagcctgggt ggcgccttac
     8761 ttgatcaggt caatgccgat ctggcgccgt tgatgcgcat tcatcaatgg ctgatcaaag
     8821 catgcgctcg gcaactgcat ggtttttacg ctcaggtatg gcaacgcctg caggcagagg
     8881 acgagcataa tccggtgaac ttcctggcct ttcttggcgc tgtgcaacca gtaattgcac
     8941 acgccgaggc gggcatcatc gaagaactgg acgcagtgct ggcagacgcc tggcagcagg
     9001 tcctgagcga taaacacgac cctgaacagg tgcagttgac ccatgaggac atagagcggc
     9061 tgattgtcga gctgaacacg cgtctggatg ttcggacgtt cagcgtattt ggcagccact
     9121 ttcactctcc ggatttcctg atttccagta cctcgacaga cgcgctgaac cagggcgact
     9181 actcaatcat tctgggagag gtccatccgg gcgtgcacac cctcagtcag ccggtagccg
     9241 agcctttcgg accgttcaat aaagagatcg agcaggaggt tcggcaaata ttcgacggac
     9301 ctcgtctggt gcttgccgac tcgccggaca gttatcagcg ttcgcatatc gattggcctt
     9361 tgctcgagtg ctaccaacaa ctgatactgc cttctggcgg cggttgcgtg ccggcggatc
     9421 agcgctttgc cgtcggccgt gctcgtcttg tcatgaacga gggacgtttg cgcgtcgagg
     9481 atattgccgg ccaattcagc gaagacttgg tgtgcgtgta tcccactcct atgcaccgac
     9541 tgggttttgc cttggctggc agcgtcgtgg ccaagaacga ccgacgccga ataggcttgg
     9601 gcaagacctt gtacaaacgc gcctcgtggt ggttcagtcc agagcaactg ccgtgctctg
     9661 aattcagtgt ggacaagctc gacgacgtgc tggcatggcg agcgtgggcc gtcgagcacg
     9721 gtttgccccg ttatgtgttc gccaagatcg atatcgaacc caagccgatt ttcattgatt
     9781 tcgataaccc gttgtcactc gacggtgtca gtaattcgat gaagaaagcg gggcacgtca
     9841 agttttccga gatgtgtcct gctccggacc aactgtggct cgaagaaacg cgcgggcatt
     9901 tctgttgtga aatacgtaca acctttaggg acaatggagt aacgcgtgat gaataacagt
     9961 tcggccatgg acggttcgca gcaaaagctc aagagcagct tcttcctgct gtttctgaca
    10021 attttcatcc cctttggcct ggggcatttc gtttcctacc tgtttcgtac cgtcaatgcg
    10081 gtgatctacg tcgatctgca aaccgacctg agcttgcctg ccagcagtct gggtttgctg
    10141 accggcgtgt actttctgac cttcgccgcc gcgcagattc cgcttggcgt gatgctcgac
    10201 cgttatggcc cgcgcagcgt acaggcgccg atgcttttat ttgcggtcgc cggttcggtc
    10261 attttcagtg tctccagcag cgaggcaggt ctgttgatcg ggcgtggtct gatcggcctg
    10321 ggcgtggcgg gctcgctgat gagtgcgatc aaagcatgcg ccatctggtt gccggtcgag
    10381 cgtttgccgc tgagcaccgc ctgcctgctg tcgattggcg gactgggcgc aatggcgtcg
    10441 accacgccac tgcatgcgct gctgagctgg ttgacctggc gcgaagcgtt tctggtcctg
    10501 gcgctgctga ccttttgcgt ggccgtggtc atacatttca gcgtgcccaa aacctaccag
    10561 agcaaaaaca ctcgctacgg cgatatgttc gccgcagtcg gcaaactgta ctcgtcctgg
    10621 acgttctggc gtctggcgtt gtactcggtg ttcgcacacg ccatctacat gtcggtgctg
    10681 tcattgtgga tggggccgtg gttgcgcgac atggcaggtt tgagtgattc cgcgatggcc
    10741 agtgtgctgt tgttcggggc catcgcgata gtggcggggt ctctgacgtt cggcgcgatc
    10801 accgattacc tgcgcaggtt cggcgtacag ccgatcatga tctgtggcac cgggatgctg
    10861 atctttatcg gcttccaggt gctgatggtc agcgacttgc cggtatcgcc ttatctgatt
    10921 gccatgggct tcagcttctt cggcacctcg accaccatga attacgccat cgtcgcgcag
    10981 tcggtgtcgc cagagctggc gggcagggtg agttcatcct tcaatctggt ggtgttcgtg
    11041 ctcgtgtttt gcctgcaatg gctgatgggc gtggtgctca atctgtggcc ggtcgcgcag
    11101 ggagcggggc atccggctga ggcctatcaa tggtcgttgg ggctgatgat tgccctgcaa
    11161 gtgccgggtt tgctgctttg gctcagcttt cggccatggc agcgcaaggt ctga
//
