LOCUS       BGC0001493              2811 bp    DNA     linear   CON 16-APR-2022
DEFINITION  Streptomyces achromogenes subsp. achromogenes strain NRRL B-2120
            contig2.1, whole genome shotgun sequence.
ACCESSION   BGC0001493
VERSION     BGC0001493
KEYWORDS    .
SOURCE      Streptomyces achromogenes subsp. achromogenes
  ORGANISM  Streptomyces achromogenes subsp. achromogenes
            Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
            Streptomyces.
COMMENT     REFSEQ INFORMATION: The reference sequence is identical to
            JODT01000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-08 02:04:12
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..2811
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[208:2527]"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      209..2527
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
     cand_cluster    1..2811
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /kind="single"
                     /product="lassopeptide"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..2811
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0001493"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..2811
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lassopeptide"
                     /region_number="1"
                     /rules="(PF13471 and Asn_synthase or micJ25 or mcjC)"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            70..198
                     /locus_tag="IH25_RS40430"
     CDS             70..198
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (lassopeptides)
                     predicted lassopeptide"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF033521.1"
                     /locus_tag="IH25_RS40430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="lasso RiPP family leader peptide-containing
                     protein"
                     /protein_id="WP_157846923.1"
                     /transl_table=11
                     /translation="MEKNPEVAGEEYESPIMVELGEFTELTNGIGSQTWDTIWLWD"
     CDS_motif       70..153
                     /prepeptide="leader"
                     /note="peptide class: lassopeptide"
                     /note="predicted sequence: MEKNPEVAGEEYESPIMVELGEFTELTN"
                     /locus_tag="IH25_RS40430_lassopeptide"
                     /aSTool="lassopeptides"
                     /tool="antismash"
     CDS_motif       154..198
                     /prepeptide="core"
                     /locus_tag="IH25_RS40430_lassopeptide"
                     /peptide="lassopeptide"
                     /predicted_class="Class II"
                     /score="4.90"
                     /molecular_weight="1659.8"
                     /monoisotopic_mass="1658.8"
                     /core_sequence="GIGSQTWDTIWLWD"
                     /aSTool="lassopeptides"
                     /tool="antismash"
                     /leader_sequence="MEKNPEVAGEEYESPIMVELGEFTELTN"
                     /leader_location="[69:153](+)"
     gene            209..2053
                     /locus_tag="IH25_RS0102215"
     CDS             209..2053
                     /GO_process="GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Asn_synthase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1177:asparagine synthase (glutamine-hydrolyzing)
                     (Score: 118; E-value: 7.6e-36)"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /locus_tag="IH25_RS0102215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="asparagine synthase-related protein"
                     /protein_id="WP_051753637.1"
                     /sec_met_domain="Asn_synthase (E-value: 3.3e-41, bitscore:
                     130.1, seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPDTHEPGGEGEFVVLPDCDGVRAPTAPGTEVLATHPSGRPLVVG
                     VLHGDRLLVSRTPTGTVAVLGFCPVDRRLLESRLAGARTPADMQRAASSLPGSHHVVVS
                     AGGTVRAWGTLAHTRRLFYDARALASNRADTLAVGATALDETALALRLLLPGVPHPLSE
                     TTVWQGVRSLPGDHFLELTPDGRARTGRRWSPPRPESTRAQTAVLLREALRTAVHARVE
                     TGAVVSCDLSGGLDSTPVAYLAWQRATRLVALTLTGLTSEDDDTGAAVRAAGHMPGADH
                     RLIGAGGLPHALEGLFDSRPRHDEPFQGLQSARFLELARLARSAGSRLHLTGHGGDEVL
                     TAPPSYVHTLIRQHPLSALNHLYGQRGLRRWSVRQTWRMVSDRRPYGAWLAHSARHLRS
                     PMPGQWRVPPPLWHQTLRLPPWITAQAAQAVADALTDAARRARPLAETRTQHLALENIR
                     TSGRIARLVNQLTAEAGVQTATPYLDDRVVEICLATRPEERSSPWEFKPLLREALEPAV
                     PRGMLQRSTKNDMAEDVHTALRGQRHRLAALCDDSLLAGMGLIDAGELRQACLAPQRPG
                     PLPAALILTLNCESWLRGRLSPTSRIAAGGTVPGGPGP"
     gene            2099..2527
                     /locus_tag="IH25_RS0102220"
     CDS             2099..2527
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: PF13471"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014140854.1"
                     /locus_tag="IH25_RS0102220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="WP_030601899.1"
                     /sec_met_domain="PF13471 (E-value: 4.9e-27, bitscore: 82.5,
                     seeds: 84, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTRPGDQRPLTPGRRLCAVLAVGAARLLTRRPPRDVRRVLTRLAK
                     GARPATYDEAAAARAAVLRVSVMCAGDGCLQRSVAIALLCRVRGAWPTWRVGVRTMPFR
                     AHAWVEAEGTAVGESTDTTLWRPLITVESHSRAGEVRP"
     gene            2524..2811
                     /locus_tag="IH25_RS0102225"
     CDS             2524..2811
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RRE-containing: Stand_Alone_Lasso_RRE"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RRE-containing: Lasso_Fused_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF033530.1"
                     /locus_tag="IH25_RS0102225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="lasso peptide biosynthesis PqqD family chaperone"
                     /protein_id="WP_037654159.1"
                     /sec_met_domain="PF05402 (E-value: 1.7e-16, bitscore: 48.6,
                     seeds: 461, tool: rule-based-clusters)"
                     /sec_met_domain="Stand_Alone_Lasso_RRE (E-value: 2.4e-25,
                     bitscore: 76.6, seeds: 25, tool: rule-based-clusters)"
                     /sec_met_domain="Lasso_Fused_RRE (E-value: 1.1e-09,
                     bitscore: 26.5, seeds: 20, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTRSTRLHSHVTTVATDDGLILLDERGGRYWQLNTTAAAILEHLT
                     RGSDPRTTAEHLVSRYGIGHAQAESDVHTFLEHLRSAGLLHSTPDDGTTG"
ORIGIN
        1 atggtcattg cccttcatgg caccaggccc cgtagcggct ctccgcgggc cgctggaagg
       61 aggaacacga tggagaagaa ccccgaagtg gcgggtgagg agtacgagtc cccgatcatg
      121 gtcgaactcg gggagttcac ggagctgacc aacggcatcg gttcccagac ctgggacacc
      181 atctggctgt gggactgagg tcctgaccgt gccggacacc cacgaaccgg gcggtgaggg
      241 cgagttcgtc gtccttcccg actgcgacgg cgtccgcgcc cccaccgccc cgggcaccga
      301 ggtcctggcc actcatccgt cgggacggcc gctggtcgtc ggcgtactcc acggcgaccg
      361 gctcctggtg agccgtaccc ctacgggaac cgtcgcggtg ctgggcttct gcccggtgga
      421 ccggcgcctg ctggagagcc gcctggccgg cgcacggacg ccggcggaca tgcaaagggc
      481 cgcgtccagc ctcccgggca gccatcatgt cgtcgtctcc gccggtggaa cggtccgcgc
      541 gtggggcacc ttggcccaca cccggcggct gttctacgac gcaagggcgc tggcgtcgaa
      601 tcgcgcggac accctggccg tcggcgccac cgcactggac gagaccgcac tggccctgcg
      661 gctgctcctg cccggcgtac cgcatccgct gtcggagacg accgtgtggc agggtgtccg
      721 ctccctgccc ggggaccact tcctcgaact gaccccggac ggcagggcac ggaccgggcg
      781 gcgatggagt ccgccgcgcc ccgagtcgac gcgggcgcag accgccgtcc tgctgcgtga
      841 ggctctccgc accgccgtac acgcccgggt cgagaccggc gcggtcgtca gctgcgacct
      901 gtccggcggg ctcgactcca ccccggtcgc ctatctggcg tggcagcggg cgacccggct
      961 cgtcgccctc accctgaccg gtctcaccag tgaggacgac gacaccgggg cggcggtacg
     1021 agccgcgggt cacatgccgg gcgccgacca caggctgatc ggcgcgggcg gactgcccca
     1081 tgccctggaa gggctcttcg acagccggcc ccgccatgac gagcccttcc aggggctgca
     1141 gagcgcgcgc ttcctggagc tggcgcgcct cgcccggtcg gccggctccc ggctgcacct
     1201 gacgggccac ggtggtgacg aggtcctgac ggccccgccg tcgtacgtgc acacgctgat
     1261 ccggcaacac cccttgtcgg ccctcaacca cctctacgga cagaggggtt tgcggcggtg
     1321 gtcggtgcgg cagacctggc ggatggtcag cgaccgccgc ccgtacgggg cgtggctcgc
     1381 gcacagcgcc cggcacctgc gctcgccgat gcccggccag tggcgcgttc ccccgcccct
     1441 gtggcaccag accctgcggc tccccccgtg gatcaccgcg caggccgcac aggcggtggc
     1501 ggatgccctg accgacgcgg cccgccgggc ccggccgctg gccgagaccc gtacgcagca
     1561 cctggccctg gagaacatca ggacgtccgg acggatcgcc cggctggtca accagctgac
     1621 ggccgaggcg ggtgtgcaga ccgccacgcc ctatctcgac gaccgtgtgg tggagatctg
     1681 cctggcgacc cgccccgagg aacgttccag cccctgggag ttcaaaccgc tcctgcggga
     1741 agccctggag ccggccgtgc cccgcgggat gctccagcgg tccacgaaga acgacatggc
     1801 cgaggacgtc cacacggccc tgcgcggtca gcggcacagg ctcgcggcgc tgtgcgacga
     1861 ctcgctgctg gccgggatgg gactgatcga cgccggggaa ctgcggcagg cgtgcctggc
     1921 cccgcagcgg ccagggcccc tgccggctgc cctcatcctg acgctgaact gcgagtcctg
     1981 gctgcgtggc cgcctgtcgc ccacgtctcg catcgccgcc ggcggcaccg tgcccggcgg
     2041 gcccggcccg tgacacacct accggaggaa caaggtgagg aaagcgagag gtgcggccct
     2101 gacgcggccc ggggatcagc ggcccctgac tccgggacgg cgactgtgcg cggtgctggc
     2161 cgtcggcgcg gcacgcctcc tgacgcgccg gccgccccgc gacgtgcggc gcgtgctgac
     2221 ccgcttggcc aagggagcga gaccggcgac ctacgacgag gcggcggcgg cgcgagcggc
     2281 ggtcctcagg gtgagcgtca tgtgcgccgg tgacggctgt ctccagcggt ccgtggccat
     2341 cgccctgctg tgccgtgtgc ggggcgcgtg gccgacgtgg cgggtcgggg tccgtacgat
     2401 gcctttccgc gcgcacgcct gggtggaggc ggaggggacc gcggtcgggg agagcaccga
     2461 caccaccctg tggcggccac tgatcacggt cgaaagccac tcccgggcag gggaggtccg
     2521 tccatgacac ggagcacccg actgcactcc catgtgacga ccgtcgcgac cgacgacgga
     2581 ctgatcctcc tggacgagcg cggcggacgg tactggcagc tgaacaccac cgcggcggcg
     2641 atcctggagc acctcacgcg aggatcggat ccccgcacga ccgcggagca tctggtgtcc
     2701 cggtacggca tcggacacgc acaggcggaa tccgacgtcc acaccttcct cgaacacctc
     2761 cgctccgccg gcctcctgca ctccacaccg gacgacggga ccaccgggta g
//
