LOCUS       BGC0001513             11818 bp    DNA     linear   BCT 30-APR-2018
DEFINITION  Actinobacteria bacterium strain MA7150 anthrabenzoxocinones
            biosynthetic gene cluster, complete sequence.
ACCESSION   BGC0001513
VERSION     BGC0001513
KEYWORDS    .
SOURCE      Actinobacteria bacterium
  ORGANISM  Actinobacteria bacterium
            Bacteria; Actinobacteria.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-08 02:12:15
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     misc_feature    1..11698
                     /note="anthrabenzoxocinones biosynthetic gene cluster;
                     +abx"
     source          1..11818
                     /db_xref="taxon:2900548"
                     /mol_type="genomic DNA"
                     /organism="Actinobacteria bacterium"
                     /strain="MA7150"
     protocluster    1..11818
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[4169:6658]"
                     /cutoff="20000"
                     /detection_rule="(t2ks and t2clf)"
                     /neighbourhood="35000"
                     /product="T2PKS"
                     /protocluster_number="1"
                     /t2pks_malonyl_elongations="11|12 (Score: 677.9; E-value:
                     8.7e-207)"
                     /t2pks_molecular_weights="acetyl-CoA_11 (Da): 456.486"
                     /t2pks_molecular_weights="acetyl-CoA_12 (Da): 498.523"
                     /t2pks_product_classes="pentangular polyphenol"
                     /t2pks_starter_units="acetyl-CoA (Score: 0.0; E-value: 0)"
                     /tool="antismash"
     proto_core      4170..6658
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(t2ks and t2clf)"
                     /neighbourhood="35000"
                     /product="T2PKS"
                     /protocluster_number="1"
                     /t2pks_malonyl_elongations="11|12 (Score: 677.9; E-value:
                     8.7e-207)"
                     /t2pks_molecular_weights="acetyl-CoA_11 (Da): 456.486"
                     /t2pks_molecular_weights="acetyl-CoA_12 (Da): 498.523"
                     /t2pks_product_classes="pentangular polyphenol"
                     /t2pks_starter_units="acetyl-CoA (Score: 0.0; E-value: 0)"
     cand_cluster    1..11818
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(t2ks and t2clf)"
                     /kind="single"
                     /product="T2PKS"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..11818
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0001513"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..11818
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T2PKS"
                     /region_number="1"
                     /rules="(t2ks and t2clf)"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            121..1992
                     /gene="abxR2"
     CDS             121..1992
                     /codon_start=1
                     /gene="abxR2"
                     /gene_functions="regulatory (smcogs)
                     SMCOG1041:transcriptional regulator, SARP family (Score:
                     569.7; E-value: 2.1e-172)"
                     /gene_kind="regulatory"
                     /product="transcriptional regulator, SARP family protein"
                     /protein_id="AUO15556.1"
                     /transl_table=11
                     /translation="MNWGSSRMRFQVLGNFEVLTDTRAQTPSAPKLRRALALLILRHNE
                     VVPTKALIDELWGSRPPDKAIRAVHTYIYELRRGLGRPDGGELFLQTRPSGYTVRVPES
                     AIDLNSFRALVKEGREALAAGDPEHARELLNRALGLWQGSALANVDCGELLEAHATELE
                     ESRLRALEMRIEADFRLSRHQELTGELKALAAARPLHEGIHARLMLALYRSGRRGEALK
                     VFHDLRRHLVDELGLEPGPELQRLQRSILAGDPSLDPPPAAPLPPPRRPRLPAPPAQLP
                     RDIVDFTGRQAVLDEVGGLLAAHGDGTGLPVVSLVGMPGVGKTATAVHLAHAVRARYPD
                     GQLYVPLGGAQTTPASAAEGLEHILRGIGVAPRDIPATLSGRMALYRTWTSDRRMLLVL
                     DDADSPQQVEPLLPGGTGCAVLITSRSLLYGLRGARTVTLGCLSTAEGGQLLTRLIGRE
                     RTAAEPRAVAEVVRLADGLPLAITFLGERLTAMRPVSISCVLAKIRSAKGQHRLSELSA
                     LGLDLYDRLDGCFRKLDPDTQEAFLRMALMPRRLFTAGQGARALGTDTTTADVVLMRLV
                     DASLLEVAGEPAAGRRHYRLRELVHEYALERMAATRTAPAVRHFIGF"
     gene            complement(2034..2693)
                     /gene="abxR1"
     CDS             complement(2034..2693)
                     /codon_start=1
                     /gene="abxR1"
                     /gene_functions="regulatory (smcogs) SMCOG1224:TetR family
                     transcriptional regulator (Score: 156.9; E-value: 8.8e-48)"
                     /gene_kind="regulatory"
                     /product="TetR family transcriptional regulator"
                     /protein_id="AUO15557.1"
                     /transl_table=11
                     /translation="MKVLFGEGEGRRMSGEDAGAPPVRTRRRGAALERAIYEATIAECA
                     ESGYGRLTLRGVAERARTGKAALYRRWSTPQDLLLDAFEHVLLPSEELPETGDLREELV
                     AALRRMADALTGRTGPPAMVLLGDLLRHPELIAVFRTRFIEPRLEAIAEILRRAAERGE
                     IPGESASPFAARTGPAQVMMHYLLNGTPPDDRAIEAMVDQVVLPALRAGRRAPGDT"
     gene            2941..3624
                     /gene="abxO"
     CDS             2941..3624
                     /codon_start=1
                     /gene="abxO"
                     /note="protein in whiE locus"
                     /product="AbxO"
                     /protein_id="AUO15558.1"
                     /transl_table=11
                     /translation="MERVALGLPVEPGNETRVARLLRRLPAAPAGEARTETVATFTAPR
                     RVLHIADTTGGLDGLVADLTAHPDVAAGLRELCGMPHRLSAASYLIRARLTRWAHYARP
                     YPARLTVRRRALLYPVRPGKGEELRRLLAAGVAPGTSQQLASALISSTIFARQDLVVRF
                     WEATADPAEELDNIARVVPGSGLGAKLNRLLDVEQDLTTEAGFRAFFTGCAMSPVPGAG
                     AWARP"
     gene            3712..4137
                     /gene="abxR"
     CDS             3712..4137
                     /codon_start=1
                     /gene="abxR"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) MannoseP_isomer"
                     /gene_functions="biosynthetic-additional (t2pks) CYC C5-C18
                     (Score: 216.3; E-value: 2.5e-67)"
                     /gene_kind="biosynthetic-additional"
                     /note="protein in whiE locus"
                     /product="AbxR"
                     /protein_id="AUO15559.1"
                     /sec_met_domain="MannoseP_isomer (E-value: 3.3e-08,
                     bitscore: 23.2, seeds: 17, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAQPVPVVSLDDAPPNRRRGGDLRTLLSPKTVGATSGFLGVAII
                     EPGEYVAEHYHPYSEEFLYVVRGDLVVDLDGTPRELHADQGLMVPIGMRHRVRNTGAVQ
                     ARIVFHLSPLAPRPELGHVDTETVESPATGTLAGDSR"
     gene            4170..5399
                     /gene="abxP"
     CDS             4170..5399
                     /NRPS_PKS="Domain: PKS_KS(Iterative-KS) (41-383). E-value:
                     7.1e-56. Score: 181.7. Matches aSDomain:
                     nrpspksdomains_abxP_PKS_KS.1"
                     /codon_start=1
                     /gene="abxP"
                     /gene_functions="biosynthetic (rule-based-clusters) T2PKS:
                     t2ks"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022:Beta-ketoacyl synthase (Score: 471.1; E-value:
                     5.7e-143)"
                     /gene_functions="biosynthetic-additional (t2pks) KS (Score:
                     742.3; E-value: 3.1e-226)"
                     /gene_kind="biosynthetic"
                     /product="beta-ketoacyl synthase alpha"
                     /protein_id="AUO15560.1"
                     /sec_met_domain="t2ks (E-value: 1e-230, bitscore: 755.5,
                     seeds: 25, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAPGGIGTKGFWELLTAGRTATRRISLFDPTGFRSQVAAECDFDP
                     AAEGLSPQEIRRMDRAAQFAVAATREALADSGLTPAEAPAHRTGVSLGSAVGCTTGLEE
                     EYVVLSDAGRLRLVDHRYGVPQLYGYMVPSTLAVETAWACGAEGPVGVVSTGCTSGLDA
                     VGYGAQLIAEGSADVMVAGATDAPISPITSACFDAIKATSPNNDDPAHASRPFDHRRDG
                     FVLGEGAAVMVLEERGRAQARGAHVYAELAGFAGRSNAFHMTGLRPDGREMAEAIRVAL
                     DRARLAPDDVDYINAHGSGTRQNDRHETAAFKRALGERAREVPVSSIKSMVGHSLGAIG
                     AIEVAACALALEHQVVPPTANLRHPDPECDLDYVPLTARDHTMDVVLSVGSGFGGFQTA
                     MLLSRPGRAR"
     aSDomain        4293..5318
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_abxP_PKS_KS.1"
                     /domain_subtype="Iterative-KS"
                     /evalue="7.10E-56"
                     /label="abxP_PKS_KS.1"
                     /locus_tag="abxP"
                     /protein_end="383"
                     /protein_start="41"
                     /score="181.7"
                     /tool="antismash"
                     /translation="DFDPAAEGLSPQEIRRMDRAAQFAVAATREALADSGLTPAEAPAH
                     RTGVSLGSAVGCTTGLEEEYVVLSDAGRLRLVDHRYGVPQLYGYMVPSTLAVETAWACG
                     AEGPVGVVSTGCTSGLDAVGYGAQLIAEGSADVMVAGATDAPISPITSACFDAIKATSP
                     NNDDPAHASRPFDHRRDGFVLGEGAAVMVLEERGRAQARGAHVYAELAGFAGRSNAFHM
                     TGLRPDGREMAEAIRVALDRARLAPDDVDYINAHGSGTRQNDRHETAAFKRALGERARE
                     VPVSSIKSMVGHSLGAIGAIEVAACALALEHQVVPPTANLRHPDPECDLDYVPLTARDH
                     TM"
     CDS_motif       5163..5207
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_abxP_0001"
                     /evalue="8.60E-03"
                     /label="NRPS-te1"
                     /locus_tag="abxP"
                     /protein_end="346"
                     /protein_start="331"
                     /score="8.8"
                     /tool="antismash"
                     /translation="VGHSLGAIGAIEVAA"
     gene            5396..6658
                     /gene="abxK"
     CDS             5396..6658
                     /NRPS_PKS="Domain: PKS_KS(Iterative-KS) (75-415). E-value:
                     2.2e-20. Score: 64.8. Matches aSDomain:
                     nrpspksdomains_abxK_PKS_KS.1"
                     /codon_start=1
                     /gene="abxK"
                     /gene_functions="biosynthetic (rule-based-clusters) T2PKS:
                     t2clf"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1093:Beta-ketoacyl synthase (Score: 631.8; E-value:
                     6.4e-192)"
                     /gene_functions="biosynthetic-additional (t2pks) CLF 11|12
                     (Score: 677.9; E-value: 8.7e-207)"
                     /gene_kind="biosynthetic"
                     /product="beta-ketoacyl synthase beta"
                     /protein_id="AUO15561.1"
                     /sec_met_domain="t2clf (E-value: 2.6e-190, bitscore: 621.9,
                     seeds: 26, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTPAAGAATAAPTAWVTGLGIVAPGGIGVEEYWSAMLRGESGIGP
                     ITRFDASGYPVRLAGEVPGFRTEERVPGRLAVQTDRWTHLGLAAAEAALADAGAGPDAL
                     TALSEYDMAVVTASSSGGTEFGQREIERLWSRGPRHVGAYQSIAWFYAATTGQISIRHG
                     MRGPCGVIAAEQAGALDALAHARRVLRDGARLVVSGGTDASLCPYGLVAQLSSGRLSTR
                     EDPARAYVPFDAAACGHLPGEGGAMLIVEDARSARRRGAAHAYGEIAGHAATFDPPPGS
                     GREPGLRRAIVNALIDARASAEEIDVVFADAAAVPALDRAEADALARVFGPRGVPVTAP
                     KTLTGRLYAGAALDVATALLAIRDDVIPPTAAGVTPHRDHRLDLVVGEPRPGPVRTALV
                     AARGFGGMNAALVLRRTDPRG"
     aSDomain        5621..6640
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_abxK_PKS_KS.1"
                     /domain_subtype="Iterative-KS"
                     /evalue="2.20E-20"
                     /label="abxK_PKS_KS.1"
                     /locus_tag="abxK"
                     /protein_end="415"
                     /protein_start="75"
                     /score="64.8"
                     /tool="antismash"
                     /translation="AVQTDRWTHLGLAAAEAALADAGAGPDALTALSEYDMAVVTASSS
                     GGTEFGQREIERLWSRGPRHVGAYQSIAWFYAATTGQISIRHGMRGPCGVIAAEQAGAL
                     DALAHARRVLRDGARLVVSGGTDASLCPYGLVAQLSSGRLSTREDPARAYVPFDAAACG
                     HLPGEGGAMLIVEDARSARRRGAAHAYGEIAGHAATFDPPPGSGREPGLRRAIVNALID
                     ARASAEEIDVVFADAAAVPALDRAEADALARVFGPRGVPVTAPKTLTGRLYAGAALDVA
                     TALLAIRDDVIPPTAAGVTPHRDHRLDLVVGEPRPGPVRTALVAARGFGGMNAALVLRR
                     "
     gene            6677..6943
                     /gene="abxS"
     CDS             6677..6943
                     /NRPS_PKS="Domain: ACP (29-75). E-value: 2.6e-13. Score:
                     41.9. Matches aSDomain: nrpspksdomains_abxS_ACP.1"
                     /codon_start=1
                     /gene="abxS"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1129:acyl carrier protein (Score: 110.7; E-value:
                     4.5e-34)"
                     /gene_functions="biosynthetic-additional (t2pks) ACP
                     (Score: 115.9; E-value: 1.4e-36)"
                     /gene_kind="biosynthetic-additional"
                     /product="ACP"
                     /protein_id="AUO15562.1"
                     /sec_met_domain="PP-binding (E-value: 2.4e-12, bitscore:
                     37.0, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTEFTIDDLRRIMRGSAGAEDGERLDGDALDTDFADLGYDSLAVL
                     EIHSQIENTFDLTLPDDALSRMRTPRETVSVVNACIGNIGEGE"
     aSDomain        6764..6901
                     /aSDomain="ACP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_abxS_ACP.1"
                     /evalue="2.60E-13"
                     /label="abxS_ACP.1"
                     /locus_tag="abxS"
                     /protein_end="75"
                     /protein_start="29"
                     /score="41.9"
                     /tool="antismash"
                     /translation="LDTDFADLGYDSLAVLEIHSQIENTFDLTLPDDALSRMRTPRETV
                     S"
     gene            6952..7443
                     /gene="abxC"
     CDS             6952..7443
                     /NRPS_PKS="Domain: Polyketide_cyc2 (13-155). E-value:
                     3.5e-18. Score: 58.2. Matches aSDomain:
                     nrpspksdomains_abxC_Polyketide_cyc2.1"
                     /codon_start=1
                     /gene="abxC"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1128:cyclase/dehydrase (Score: 202.5; E-value:
                     1.6e-61)"
                     /gene_functions="biosynthetic-additional (t2pks) CYC C9-C14
                     (Score: 246.4; E-value: 1.9e-76)"
                     /gene_kind="biosynthetic-additional"
                     /product="cyclase"
                     /protein_id="AUO15563.1"
                     /transl_table=11
                     /translation="MTEATGAGATEHARTDNSIVIDAPLELVWDITCDVEGWPRLFSEY
                     ESVEILHREGDTIRFRLTMHPDEDGNRWSWVSERTADPATHTVRSRRVETGPFAFMNIF
                     WEFTPDPGGVRMRWVQEFHMSPGAPVDDAAMAEHINRNTRIQMELIRDRIEARARASAP
                     "
     aSDomain        6991..7416
                     /aSDomain="Polyketide_cyc"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_abxC_Polyketide_cyc2.1"
                     /domain_subtype="Polyketide_cyc2"
                     /evalue="3.50E-18"
                     /label="abxC_Polyketide_cyc2.1"
                     /locus_tag="abxC"
                     /protein_end="155"
                     /protein_start="13"
                     /score="58.2"
                     /tool="antismash"
                     /translation="RTDNSIVIDAPLELVWDITCDVEGWPRLFSEYESVEILHREGDTI
                     RFRLTMHPDEDGNRWSWVSERTADPATHTVRSRRVETGPFAFMNIFWEFTPDPGGVRMR
                     WVQEFHMSPGAPVDDAAMAEHINRNTRIQMELIRDRIE"
     gene            7552..7893
                     /gene="abxD"
     CDS             7552..7893
                     /codon_start=1
                     /gene="abxD"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1225:Polyketide synthesis cyclase (Score: 113.8;
                     E-value: 6.3e-35)"
                     /gene_functions="biosynthetic-additional (t2pks) CYC
                     C4-C21/C2-C23|C2-C19 (Score: 132.2; E-value: 1.5e-41)"
                     /gene_kind="biosynthetic-additional"
                     /product="cyclase"
                     /protein_id="AUO15564.1"
                     /transl_table=11
                     /translation="MATTLIVARLKPGDHRDQISRLFAESDTTELPDLVGVQERRLLTF
                     KDLYFHLVRTDEALSKTLTPQHDHPLFRSISEAMDEYVTPYEGAWGSVEQASARQFYHW
                     KRGLGRVQP"
     gene            7922..8968
                     /gene="abxM"
     CDS             7922..8968
                     /codon_start=1
                     /gene="abxM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1042:O-methyltransferase (Score: 287.1; E-value:
                     3.2e-87)"
                     /gene_functions="biosynthetic-additional (t2pks) MET C6|C8
                     (Score: 474.0; E-value: 4.8e-145)"
                     /gene_kind="biosynthetic-additional"
                     /product="methyltransferase"
                     /protein_id="AUO15565.1"
                     /transl_table=11
                     /translation="MTDTDYTPELATAARVKELAMGLWVSAMMMTAVQLRLPDEIDDKP
                     VEAAELAARLDLHPEAVARLLRALAAHGVFAEPAEDRFAHTELSRVLREDHPHSVRYQV
                     LWTVAPWTWRAWPHLEEAVRTGERVFPRLYGKEFFAYLAEDAPRSAEVFNRAMTQSSTF
                     TSAMVAEALDMTGVRVVVDIGGGRGHLLRTVLERYPWLSGVLYDLPSVVASADPALRPG
                     GELADRTRVVAGSCLDGVPPGDLYILKNLLEWDDERTVTTLRNVAAALRSGGRAVLVQN
                     LVDDSPEPKVTTGMDLLLLLNVGGRKHTRRQLTRLLSEAGLEITDIRPTASSLFLIEVA
                     RRAPAAGA"
     CDS             complement(8965..10443)
                     /codon_start=1
                     /note="orf14"
                     /product="membrane protein"
                     /protein_id="AUO15566.1"
                     /transl_table=11
                     /translation="MEWFTSSDYWLTRLVFQRGLAALYVIAFVVAANQGRALIGERGLT
                     PVPRFLARMPFRGSPSLFHLHFSDRFLTGCAWLGAALAAAVAAGAADRVPLWAAMMMWL
                     VLWLLYLSIVNVGQVWYGFGWESLLLETGFLAVFLGNARTAPPVLVLWLLRWLLFRVEF
                     GAGLIKIRGDRCWRQLTCLYYHHETQPMPGPLSWFFHRLPKPLHRVEVAANHLAQLVVP
                     FALFTPQPVASVAGGVVVVTQLWLVASGNFSWLNWVTVLLGLAAVDGRRAARVLGLPEP
                     PPLAAPPIWYEAVVLAATALVLVLSYWPARNLISGRQLMNYSFNPLHLVNTYGAFGSVN
                     RSRFEVVIEGTDEPVPTEDTVWKEYEFRGKPGDVRRMPRQFAPYHLRLDWMMWFAGISP
                     AYARAWFGPLVTKLLEGDRATLRLLRSCPFPGAPPTYLRARLFLYRFTTWRELRATGAW
                     WHRTFVREFLPPVALDRTGGADRPDGPGGGSGAG"
     CDS             complement(10652..11698)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1078:LacI family
                     transcription regulator (Score: 275.8; E-value: 8.8e-84)"
                     /gene_kind="regulatory"
                     /note="orf15"
                     /product="LacI-like transcription regulator"
                     /protein_id="AUO15567.1"
                     /transl_table=11
                     /translation="MDSEVTAGPPRRVTIKDVAARAGVSKGAVSLAFNDRPGVSQATRA
                     RIFEAARELGWAPHLSARSLSNARVDAVGLAIARPARMLGLEPWFMEFVSGIESVLAER
                     SCSLLLRLVRDVREEIAVQEAWWRARQVGGSILVDLHDQDPRVPALRGLGLPAVAAGHP
                     SLAGGFTCVWTDDATAVGEAVRYLAALGHRRIARVSGPASLGHSVIRTAAFEAAVRQLG
                     LADGRTVATDFSGEEGARATRALLSSADRPTAVLYDNDIMAVAGLSVTAEMGLSVPADV
                     SLLAWDDSQLCQLTHPTLSAMSHDVFGFGAEVARRLFDVLDQAAPGSHQAPTPALTPRG
                     STAPPRRP"
ORIGIN
        1 tcagcgggca caggtgcgat tgagccgaaa tatggtgcgg atccactgat catgcgggcc
       61 ggaggtatgc tcggctgcga cctgaaatac ggggcagggg cagggccttg tgacgccaca
      121 atgaactggg ggagttcgcg tatgaggttc caggtgctgg gaaacttcga agtcctgacc
      181 gacacccgcg cccagacgcc gagcgctccc aagctccgcc gggccctcgc cctgctgata
      241 ctgcggcaca acgaggtcgt gcccaccaag gccctcatcg acgaactgtg ggggagcagg
      301 ccccccgaca aagccatccg tgccgtacac acctacatct acgaactgcg ccgcggcctc
      361 ggccgcccgg acggcggaga gctgttcctc cagacccgcc cgagcggtta taccgtccgc
      421 gtgccggaaa gcgcgatcga tctcaattcc ttccgggccc tggtcaagga aggcagggag
      481 gcactcgccg cgggggaccc ggaacacgcc cgggaactgc tgaaccgggc cctgggactc
      541 tggcagggca gcgccctggc caatgtggac tgcggggaac tgctcgaagc gcacgcgacc
      601 gagctggagg aaagccggct gcgcgccctg gagatgcgca tcgaggccga tttccggctc
      661 agccgccacc aggaactgac cggggagctg aaggccctcg ccgccgccag gcccctgcac
      721 gagggcatcc acgcccggct gatgctcgcg ctctaccgct cgggacgccg cggcgaggcg
      781 ctcaaggtct tccacgacct gcgccgccac ctggtcgacg agctgggcct ggagcccggc
      841 cccgaactcc agcgcctgca gcgctccatc ctcgccgggg acccgtcact ggacccgccg
      901 cccgccgccc cgctcccgcc gccccgccgc ccccgcctcc cggccccgcc cgcccagctc
      961 ccccgggaca tcgtcgactt caccgggcgg caggccgtgc tcgacgaggt cggcggcctg
     1021 ctcgcggccc acggggacgg caccggcctg cccgtggtgt ccctggtcgg catgccgggc
     1081 gtcggcaaga cggccaccgc cgtccacctc gcccacgcgg tccgcgcccg ctaccccgac
     1141 ggccagctgt acgtcccgct gggcggagcg cagaccacac ccgcgtccgc cgcggaagga
     1201 ctggagcaca tcctgcgggg catcggcgtg gcgccccgcg acatcccggc caccctgagc
     1261 ggacgcatgg cgctgtaccg cacctggaca tccgaccgcc ggatgctgct ggtgctggac
     1321 gacgccgact cgccccagca ggtcgaaccg ctgctccccg gcggcacggg ctgcgccgtg
     1381 ctgatcacca gccgctccct gctgtacggg ctgcgcgggg cgcgcaccgt gacgctgggc
     1441 tgcctgtcca ccgccgaggg cgggcagctg ctgacccggc tgatcgggcg ggagcggacc
     1501 gccgccgagc cccgggccgt cgccgaggtg gtgcggctcg cggacggcct gccgctggcc
     1561 atcaccttcc tcggcgagcg gctgacggcg atgcggccgg tcagtatttc ctgcgtcctg
     1621 gcgaagatac ggtccgccaa gggccagcac cgcctctccg agctctcggc gctgggcctg
     1681 gacctctacg accggctgga cggctgcttc cgcaagctcg acccggacac ccaggaggcc
     1741 ttcctgcgca tggcgctgat gccgcgccgg ctgttcaccg ccgggcaggg cgcccgggcc
     1801 ctgggcaccg acaccaccac ggcggacgtg gtgctgatgc ggctggtcga cgcctcgctg
     1861 ttggaggtcg cgggggagcc ggcggccggc cgtcgccact accgcctccg cgaactggtc
     1921 cacgagtacg cgctggagcg gatggcggct acccgcaccg cgcccgccgt gcgccacttc
     1981 atcggcttct gacggcccgg gcgcccccgg ctcccctcgg gcggcgacga gcctcaggtg
     2041 tcccccgggg cccgccgccc ggcccgcagc gcgggcagca ccacctggtc caccatggcc
     2101 tcgatggccc ggtcgtcggg cggggtgccg ttgagcaggt aatgcatcat cacctgcgcc
     2161 ggcccggtcc gcgcggcgaa cgggctggcc gactccccgg ggatctcgcc gcgctcggcc
     2221 gcccgccgca ggatctccgc gatggcctcc agcctcggct cgatgaaccg cgtgcggaac
     2281 accgcgatca gctccggatg gcgcagcagg tccccgagca gcaccatcgc gggcggtccc
     2341 gtgcgcccgg tgagggcgtc ggccatccgc cgcagcgccg ccaccagctc ctcgcgcaga
     2401 tcgcccgtct cgggcaactc ctcgctgggc agcaggacat gctcgaaggc gtccagcagc
     2461 agatcctgcg gagtgctcca ccgccggtac agcgcggcct tcccggtgcg cgcccgctcg
     2521 gccacccccc ggagcgtcag cctgccgtag ccggactcgg cgcactcggc gatcgtggcc
     2581 tcgtagatgg cccgctccag ggcggcgccc ctgcggcggg tccgcaccgg cggcgccccc
     2641 gcgtcctccc cgctcatccg gcggccctcc ccctccccga aaagcacctt cacgacccct
     2701 ttgcggaccc tatcagcggg ggctaccctg ccggacaagg aggataagga acagttccgt
     2761 ctactatcac ggcccgtccc ctccacgaca ccgcccttgt gagaaacctg tccgcttcct
     2821 gagcgtcggt gtgcactctc gccacatcgg ctctttccgg cttcccggac gcgcccggtc
     2881 ccgggccgcg acgagccgag cgccgcacac gcagccgcac gcacaggggg agtggtacgg
     2941 atggagcggg tcgccctggg acttccggtc gagcccggca acgagacacg ggtcgcacgg
     3001 ctgctgcgac gcctccccgc ggccccggcc ggcgaggcgc gcaccgagac ggtcgccacc
     3061 ttcaccgcgc cgcgccgggt gctgcacatc gccgacacca ccggcgggct cgacggcctc
     3121 gtcgccgacc tcaccgcgca cccggacgtc gcggcaggcc tgcgcgagct gtgcggcatg
     3181 ccccaccggc tctcggcggc ctcctacctc atccgggccc ggctgacccg ctgggcccac
     3241 tacgcccggc cgtaccccgc gcggctcacc gtgcgccgcc gggccctgct ctaccccgta
     3301 cggccgggca agggcgagga gctgcgccgg ctgctggccg ccggagtcgc ccccggcacc
     3361 tcccaacagc tggcgtccgc cctgatcagc agcaccatct tcgcccgcca ggacctcgtc
     3421 gtccggttct gggaggccac cgccgacccg gccgaggagc tcgacaacat cgcccgcgtc
     3481 gtccccggct caggcctggg agccaaactc aaccggctcc tcgacgtcga gcaggacctg
     3541 accaccgagg ccggcttccg cgccttcttc acgggctgcg cgatgtcccc cgtgcccggc
     3601 gcgggcgcct gggcccggcc gtagccgccc ggcgccccac cggccgcgcc cgggcgaccc
     3661 gggcgcgccc gggcgaccca cctcaccacc gtcacttctt caggaggaag cgtgaccgca
     3721 cagcctgtcc ccgtcgtctc gctggacgac gccccgccca accgccggcg cggcggcgac
     3781 ctgcgcaccc tgctcagccc caagacggtc ggcgccacct ccggcttcct cggcgtggcc
     3841 atcatcgagc ccggcgagta cgtggccgag cactaccacc cctactccga ggagttcctg
     3901 tacgtggtgc gcggcgacct cgtcgtcgac ctcgacggca caccccgcga actccacgcc
     3961 gaccaggggc tgatggtccc catcgggatg cgccaccggg tccgcaacac cggagccgtc
     4021 caggccagga tcgtcttcca cctctcgccg ctggcccccc gcccggagct gggccatgtg
     4081 gacaccgaga cggtcgagag cccggccacc ggcacgctcg ccggggactc gcggtgaacc
     4141 gaaccgccgt catcaccggg atcggcgtgg tggcccccgg cgggatcggc accaagggct
     4201 tctgggagct gctcaccgcc gggcgcaccg ccacccggcg gatctccctc ttcgacccca
     4261 ccggattccg ctcccaggtg gccgccgaat gcgacttcga ccccgccgcg gaagggctga
     4321 gcccccagga gatacgccgg atggaccggg ccgcccagtt cgcggtcgcc gccaccaggg
     4381 aggcgctcgc cgacagcggt ctgacccccg ccgaggcgcc cgcccaccgc accggcgtca
     4441 gcctgggcag cgccgtgggc tgcaccaccg gcctggagga ggagtacgtc gtgctcagcg
     4501 acgccggccg gctccgtctg gtggaccacc gctacggggt cccgcagctg tacggctaca
     4561 tggtgcccag caccctggcc gtcgagacgg cgtgggcgtg cggcgccgag ggcccggtcg
     4621 gcgtggtctc caccggctgc acctcggggc tcgacgccgt cggctacggg gcccagctca
     4681 tcgccgaggg ctcggccgac gtgatggtcg ccggcgccac cgacgcgccc atctcgccca
     4741 tcacctcggc ctgcttcgac gccatcaagg ccacctcccc caacaacgac gacccggccc
     4801 acgcctcccg cccgttcgac caccgcaggg acggcttcgt gctcggcgag ggcgccgcgg
     4861 tgatggtgct cgaggagcgc ggccgcgccc aggcccgggg cgcccacgtc tacgccgagc
     4921 tcgcggggtt cgccggccgc agcaacgcct tccacatgac cggactgcgg cccgacggcc
     4981 gggagatggc cgaggccatc cgcgtcgcgc tcgaccgggc gcgcctcgcg ccggacgacg
     5041 tcgactacat caacgcgcac ggctccggca cccgccagaa cgaccgccac gagaccgccg
     5101 ccttcaagcg cgcgctgggc gagcgggccc gcgaggtgcc cgtcagctcc atcaagtcca
     5161 tggtcgggca ctcgctgggg gcgatcggcg cgatcgaggt ggccgcctgc gcgctggcgc
     5221 tggagcacca ggtcgtgccg cccaccgcca atctgcgcca ccccgacccg gagtgcgacc
     5281 tggactacgt cccgctgacg gcccgtgacc acaccatgga cgtggtgctg tcggtcggca
     5341 gcggcttcgg cggcttccag accgcgatgc tgctcagccg cccagggagg gcccggtgac
     5401 ccccgcggcc ggggccgcca ccgccgcccc caccgcctgg gtcaccggcc tcggcatcgt
     5461 ggcgcccggc ggcatcggcg tcgaggagta ctggtcggcg atgctgcgcg gggagagcgg
     5521 catcgggccc atcacccgct tcgacgcctc cggctatccg gtgcggctgg ccggcgaggt
     5581 gcccggcttc cgcaccgagg agcgcgtccc cggccggctc gccgtccaga ccgaccgctg
     5641 gacccatctg ggcctggccg cggccgaggc cgcgctcgcc gacgccggag ccggccccga
     5701 cgccctgacc gcgctctccg agtacgacat ggcggtcgtc accgcctcct cctccggcgg
     5761 caccgagttc gggcagcggg agatcgaacg gctgtggtcg cgcgggcccc ggcacgtcgg
     5821 cgcgtaccag tcgatcgcct ggttctacgc ggcgaccacc ggccagatct ccatccgcca
     5881 cggcatgcgc ggaccgtgcg gggtcatcgc ggccgagcag gccggcgccc tggacgcgct
     5941 cgcgcacgcc cgccgggtgc tgcgcgacgg cgcccggctc gtggtcagcg gcggcaccga
     6001 cgcctcgctg tgcccgtacg gccttgtcgc ccagctctcc agcggccggc tgtccacccg
     6061 cgaggacccg gcgcgggcct atgtgccgtt cgacgccgca gcctgcggcc acctcccggg
     6121 cgagggcggg gcgatgctca tcgtcgagga cgcccgctcg gcccggcggc gcggcgccgc
     6181 ccacgcgtac ggcgagatcg ccgggcacgc ggccaccttc gacccgccac ccggctccgg
     6241 ccgcgagccc gggctgcgcc gcgccatcgt gaacgccctg atcgacgcgc gggcgtccgc
     6301 cgaggagatc gacgtcgtct tcgcggacgc cgccgccgta cccgccctcg accgcgccga
     6361 ggcggacgcc ctcgcccggg tcttcgggcc ccgcggggtg cccgtcaccg cgcccaagac
     6421 gctgaccggg cggctgtacg cgggcgccgc gctggatgtg gccaccgcgc tgctggccat
     6481 ccgcgacgac gtcatcccgc ccaccgcggc cggggtgacc ccgcaccggg accaccggct
     6541 cgacctggtg gtgggcgagc cgcggcccgg ccccgtgcgt accgccctgg tggccgcccg
     6601 gggcttcggc ggcatgaacg ccgcgctggt cctccgccgg accgacccgc gcggatgagc
     6661 gagcgaggag acaccgatga ccgagttcac catcgacgac ctgaggcgga tcatgcgcgg
     6721 cagcgcgggg gccgaggacg gcgagcgcct cgacggggac gctctcgaca ccgacttcgc
     6781 cgacctcggc tacgactccc tggccgtact ggagatccac tcgcagatcg agaacacctt
     6841 cgacctgacc ctgcccgacg acgcgctcag ccggatgcgc accccacggg agaccgtctc
     6901 cgtggtcaac gcctgcatcg gaaacatcgg agaaggagag tgagccggag catgaccgaa
     6961 gccaccggag ccggagccac cgaacacgcc cgcaccgaca actccatcgt catcgacgcg
     7021 cccctggagc tggtgtggga catcacctgc gatgtcgagg gctggccgcg gctgttcagc
     7081 gagtacgagt cggtcgagat cctccaccgc gagggcgaca ccatacggtt ccgcctcacc
     7141 atgcaccccg acgaggacgg caaccggtgg agctgggtgt ccgagcgcac cgcggacccg
     7201 gccacgcaca cggtccgctc ccgccgcgtg gagaccggcc ccttcgcgtt catgaacatc
     7261 ttctgggagt tcaccccgga ccccggcggg gtgcggatgc gctgggtcca ggagttccac
     7321 atgtcgcccg gggcgcccgt ggacgacgcc gccatggccg agcacatcaa ccgcaacacc
     7381 aggatccaga tggagctgat ccgcgaccgg atcgaggccc gcgcccgcgc ctcggcgccc
     7441 tgaacgagcc accccaccgg gacaccccat cggacacccc cctggggaca ccccctgggg
     7501 acacccatcg acaccacccc tcgggacacc atcgagacac ggaggaaccc catggccacg
     7561 accctgatcg tcgcccggct gaagcccggc gaccaccggg accagatcag caggctgttc
     7621 gcggagtcgg acaccaccga gctgcccgac ctggtgggcg tccaggagcg gcggctgctg
     7681 accttcaagg acctgtactt ccacctcgtc cgcaccgacg aggcactctc caagacgctc
     7741 accccccagc acgaccaccc cctgttccgg tcgatcagcg aggcgatgga cgagtacgtc
     7801 acgccgtacg aaggggcctg gggcagcgtc gagcaggcct cggcccggca gttctaccac
     7861 tggaagcgcg gcctcggccg agtccagccc tgacgaccgc accacgagag gcggaaacgc
     7921 tgtgaccgac accgactaca cccccgaact cgcgaccgcg gcccgggtga aggaactggc
     7981 gatgggcctg tgggtctccg ccatgatgat gacggccgtc cagctgcggc tccccgacga
     8041 gatcgacgac aagcccgtcg aggcggccga gctcgccgcc cggcttgacc tgcaccccga
     8101 ggccgtcgcc cggctgctgc gggcgctggc cgcgcacggc gtcttcgcgg agccggccga
     8161 ggaccgcttc gcccacaccg agctctcccg ggtgctccgc gaggaccacc cgcacagcgt
     8221 ccgctaccag gtgctgtgga ccgtggcccc ctggacctgg cgggcctggc cgcacctgga
     8281 ggaggcggtg cgcaccggcg agcgggtctt cccgcgcctg tacggcaagg agttcttcgc
     8341 ctacctcgcc gaggacgccc cgcgctcggc cgaggtgttc aaccgggcca tgacccagtc
     8401 ctccaccttc acctcggcca tggtcgccga ggccctggac atgaccggcg tacgggtcgt
     8461 ggtcgacatc ggcggcggcc ggggccacct gctgcgcacc gtcctggagc gctacccctg
     8521 gctgagcggg gtgctctacg acctgccgtc cgtcgtggcc tcggcggacc ccgcgctgcg
     8581 gcccggcggg gaactcgccg accggacgcg ggtggtggcg ggaagctgcc tggacggcgt
     8641 gccgcccggc gacctgtaca tcctgaagaa cctgctggag tgggacgacg agcggaccgt
     8701 caccaccctg cggaacgtgg ccgcggcgct gcgctcgggc ggccgggcgg tgctggtgca
     8761 gaacctggtc gacgacagcc cggaacccaa ggtcaccacc gggatggacc tgctgctgct
     8821 cctcaacgtc ggcggcagga aacacaccag gcgccagctg acccggctgc tgtccgaggc
     8881 cggcctcgag atcacggaca tccgccccac ggccagcagc ctcttcctca tcgaggtagc
     8941 ccggcgcgcc ccggcagcgg gcgcctagcc cgccccgctc ccgcccccgg gcccgtccgg
     9001 ccggtccgcg ccgccggtcc ggtcgagcgc gaccgggggc aggaactcac ggacgaaagt
     9061 gcggtgccac cacgccccgg tggcccgcag ctcacgccag gtcgtgaagc ggtacaggaa
     9121 cagccgggcg cgcaggtacg tgggcggcgc gccggggaag gggcagctcc gcagcagcct
     9181 cagcgtcgcc cggtcgccct ccagcagctt ggtgaccagc gggccgaacc aggcccgggc
     9241 gtaggcgggc gagatcccgg cgaaccacat catccagtcg agcctcaggt ggtacggggc
     9301 gaactggcgc ggcatccggc gcacatcgcc cggcttgccc cggaactcgt actccttcca
     9361 caccgtgtcc tccgtgggca ccggctcgtc ggtgccctcg atcaccacct cgaaccgcga
     9421 gcggttgacg ctgccgaagg cgccgtaggt gttgaccagg tgaagcgggt tgaaggagta
     9481 gttcatcagc tgccggccgg agatcaggtt ccgggccggc cagtagctga gcaccagcac
     9541 cagcgcggtg gcggccagca ccacggcctc gtaccagatg gggggagcgg cgagcggcgg
     9601 cggttccggc aggcccagca cccgggccgc gcgccgcccg tcgaccgcgg cgaggcccag
     9661 caggacggtc acccagttga gccaggagaa gttgccggac gcgaccagcc acagctgggt
     9721 gaccacgacc acgcccccgg ccacgctcgc caccggctgc ggggtgaaca gcgcgaaggg
     9781 caccaccagc tgggcgaggt ggttggccgc gacctccacc cggtgcaggg gcttcggcag
     9841 ccggtggaag aaccagctca gcgggcccgg catcggctgg gtctcgtggt ggtagtacag
     9901 acaggtcagc tgccgccagc agcggtcccc ccggatcttg atcagcccgg cgccgaactc
     9961 cacccggaac agcagccagc gcagcagcca cagcaccagg acgggcgggg cggtgcgggc
    10021 gttgccgagg aagacggcga ggaagccggt ctccagcagc agcgactccc agccgaagcc
    10081 gtaccagacc tgcccgacat tgacgatcga cagatacagc agccacagca ccagccacat
    10141 catcatcgcg gcccacagcg gcacccggtc cgcggccccc gccgccaccg cggcggcgag
    10201 cgcggccccc agccaggcgc agccggtgag gaagcggtcc gagaagtgga ggtggaagag
    10261 gctgggggag ccccggaacg gcatccgcgc gaggaaccgc ggcaccggcg tcaggccccg
    10321 ctcaccgatc agggcccggc cctggttcgc cgcgaccacg aaggcgatca cgtagagggc
    10381 ggcaagcccc cgctggaaga ccagcctggt cagccagtag tcggatgagg tgaaccactc
    10441 catgcggaac cgctccctgg gcctgtgcca cggctcggtc cgcctccggg acgcctgagc
    10501 cccgctcgac gggcgtgcct gcccggcccc tcgctgtccg gtcgtccggc tatccgtacc
    10561 acctgagccg gacgcgtgct ccctgacggg tcccacgcgg ggcggagttc atccggtcgg
    10621 agcgccccgc gcccggccgc gcttaaccgg ttcagggccg ccggggcggg gcggtgctgc
    10681 cgcgcggggt gagcgccggg gtgggcgcct gatgggagcc gggcgccgcc tggtcgagca
    10741 cgtcgaacag gcggcgggcg acctcggccc cgaagccgaa gacgtcatgg ctcatcgccg
    10801 acagcgtggg gtgggtgagc tggcacagct gggagtcgtc ccaggccagc agcgagacgt
    10861 cggccggcac cgaaaggccc atctcggcgg tcaccgacag cccggccacc gccatgatgt
    10921 cgttgtcgta gaggaccgcc gtcggccggt cggccgagga cagcagcgcc cgggtggcgc
    10981 gtgccccctc ctccccggag aagtcggtgg cgaccgtgcg cccgtcggcc aggcccaact
    11041 ggcgcacggc cgcctcgaag gcggcggtgc ggatcacgct gtgcccgagc gaggcggggc
    11101 cgctcacccg ggcgatccgc cggtggccca gcgccgccag atagcgcacc gcctcgccga
    11161 cggcggtggc gtcgtcggtc catacgcagg tgaagccgcc ggccagggac gggtgtccgg
    11221 cggcgacggc gggcagcccg agcccgcgca gcgcggggac gcgcggatcc tggtcgtgga
    11281 ggtcgacgag gatcgacccg ccgacctgcc gcgcccgcca ccacgcctcc tggacggcga
    11341 tctcctcccg cacgtcccgc accagccgca gcagcagcga gcaggaccgc tcggcgagca
    11401 cgctctcgat gcccgagacg aactccatga accacggctc gagccccagc atcctggccg
    11461 gccgcgcgat ggccaggccc acggcgtcca cccgcgcgtt ggacagcgac cgggcggaca
    11521 gatggggcgc ccagcccagc tcccgggccg cctcgaagat ccgggcccgg gtggcctggg
    11581 agacgccggg gcggtcgttg aaggcgagcg agaccgcccc cttggagacc ccggcccgcg
    11641 cggcgacgtc cttgatggtg acgcgccgcg gtgggcccgc cgtgacctcg ctgtccatcc
    11701 accgactccc gctgctcggc tgccgcccgc cgctcgcggc gaacgccaca ccctaccgcg
    11761 ccggtaaacc ggtcaaggtg ggttttcgcg gggccgccgc tcaggacgcg gggagccc
//
