LOCUS       BGC0001615             10987 bp    DNA     linear   BCT 05-AUG-2016
DEFINITION  Heteroscytonema crispum UCFS10 mys gene cluster, complete sequence.
ACCESSION   BGC0001615
VERSION     BGC0001615
KEYWORDS    .
SOURCE      Heteroscytonema crispum UCFS10
  ORGANISM  Heteroscytonema crispum UCFS10
            Bacteria; Cyanobacteria; Nostocales; Heteroscytonemataceae;
            Heteroscytonema.
COMMENT     ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2022-11-08 02:53:55
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 10987
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..10987
                     /db_xref="taxon:1885250"
                     /mol_type="genomic DNA"
                     /organism="Heteroscytonema crispum UCFS10"
                     /strain="UCFS10"
     misc_feature    1..10987
                     /note="mys gene cluster"
     subregion       1..10987
                     /aStool="mibig"
                     /contig_edge="True"
                     /label="NRP"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..10987
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            36..491
                     /locus_tag="UCFS10_04335"
     CDS             36..491
                     /codon_start=1
                     /locus_tag="UCFS10_04335"
                     /product="hypothetical protein"
                     /protein_id="ANY58990.1"
                     /transl_table=11
                     /translation="MADQEQIDLEAQLNRVDPDKEAQPEDLSEQISDLPQAITESYGTG
                     VKDMPGYNIGGRSMRDERQNYTETSPELTGGDVDAYWQDADAVGDEAVGGTVATPDQNV
                     TEEIEAAVGLEMDDRAFLRTNDILEERDDRRWELDPTSSEDYQERRD"
     gene            1378..2607
                     /gene="mysA"
     CDS             1378..2607
                     /codon_start=1
                     /gene="mysA"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     mycosporine-like: DHQ_synthase"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     amglyccycl: DHQ_synthase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1183:3-dehydroquinate synthase (Score: 278.9; E-value:
                     8.8e-85)"
                     /gene_kind="biosynthetic"
                     /product="3-dehydroquinate synthase"
                     /protein_id="ANY58986.1"
                     /sec_met_domain="DHQ_synthase (E-value: 2.6e-61, bitscore:
                     195.9, seeds: 76, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSVPYATFAATETAFHVTGYEKIEFSLVYVNGAFDIKNTEIADSY
                     QNFGRCLLVVDANVNRLYGEQIRSYFQFYNIKLTVFPITISEPAKSLTTFEEVIDAFAD
                     FGLIRKEPVLVVGGGLVTDVVGFACAAYRRSTNYIRIPTTLIGLIDAGVAIKVAVNHKK
                     LKNRLGAYHAPQKVILDFSFLETLPIAQIRNGMAELVKIAVVANAEVFELLYEYGHELL
                     HTHFGYVNGSKELQEIAHRLNYAAIKTMLELETPNLRELDLDRVIAYGHTWSPTLELAP
                     QVPLYHGHAVNIDMALSATIAHRRGYINLEQRDRILNLMSRIGLALDHPLLDSDLLWYA
                     TQSITQTRDGKLRAAMPKPIGECFFVNDLTREELDEALAEHKLLCAELPGGGEGIDAYI
                     GTEESELIKA"
     gene            2764..3654
                     /gene="mysB"
     CDS             2764..3654
                     /codon_start=1
                     /gene="mysB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     mycosporine-like: Methyltransf_3"
                     /gene_kind="biosynthetic"
                     /product="O-methyltransferase"
                     /protein_id="ANY58987.1"
                     /sec_met_domain="Methyltransf_3 (E-value: 1e-55, bitscore:
                     177.1, seeds: 4, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDWFAQSKILSSKIQNGKTVTSIVEISTARPVTPHGILVEELRKT
                     LEMAEAASIPAEILATLRSTYQLAAGLDPYISECTTTESSALTALARKTSAEDWRKRFG
                     DGETVRQLEQEMLSGHLEGQTLKMFVRMTKAKRVLEVGMFTGYSALAMAEALPDDGKLV
                     ACEVDAYVANFAQNCFADSPHGDKITVEVAPALETLHKLAAAGESFDLIFIDADKKEYV
                     EYFQTILDSNLLAPDGLICVDNTLLQGQPYLPPEKRTANGEAIATFNSIVAQDSRVEQV
                     LLPVRDGLTLIRRLD"
     gene            3655..5046
                     /gene="mysC"
     CDS             3655..5046
                     /codon_start=1
                     /gene="mysC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     mycosporine-like: ATP-grasp_3"
                     /gene_kind="biosynthetic"
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="ANY58985.1"
                     /sec_met_domain="ATP-grasp_3 (E-value: 4.7e-09, bitscore:
                     25.4, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAQSIPFDSASPTPQVSWGVRISALWKTVGTLLLLFLALPVNASI
                     VLISLLWGIFSKPFEKRVVAAAPKNILISGGKMTKALQLARSFHAAGHRVVLVESHKYW
                     LTGHQFSNAVSVFYTVSPPEKDPEGYTQQLLDIVKKERIDVYVPVCSPVASYYDSLVKP
                     ALSQHCEVFHCDAEITQMLDDKYAFSEKARSFGLSVPKSFKITNPEQVINFDFSQEKRK
                     YILKSIPYDSVRRLNLTKLPCDTPEETAAFVRSLPISPEKPWIMQEFIPGKEFCTHSTV
                     RNGELRLHCCCESSAFQVNYENVNNPQILEWVKHFIKEMGITGQVSFDFIQTEDGTVYA
                     IECNPRTHSAITMFYNHPGVADAYLGKIPLPEPLQPLADSKPTYWLYHEIWRLTGIRSL
                     SQFWTWLKNLMRGKDAIYQLNDPLPFLTVPHWQITLLLLQNLRQLRGWVKIDFNIGKLV
                     ELGGD"
     gene            5233..9219
                     /gene="mysE"
     CDS             5233..9219
                     /NRPS_PKS="Domain: Condensation_LCL (16-307). E-value:
                     1.3e-67. Score: 219.8. Matches aSDomain:
                     nrpspksdomains_mysE_Condensation_LCL.1"
                     /NRPS_PKS="Domain: AMP-binding (472-872). E-value:
                     7.5e-124. Score: 405.2. Matches aSDomain:
                     nrpspksdomains_mysE_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (981-1050). E-value: 2.4e-22. Score:
                     70.8. Matches aSDomain: nrpspksdomains_mysE_PCP.1"
                     /NRPS_PKS="Domain: Thioesterase (1069-1307). E-value:
                     1.1e-36. Score: 119.2. Matches aSDomain:
                     nrpspksdomains_mysE_Thioesterase.1"
                     /codon_start=1
                     /gene="mysE"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127:condensation domain-containing protein (Score:
                     257.2; E-value: 5.6e-78)"
                     /gene_kind="biosynthetic"
                     /product="non-ribosomal synthetase"
                     /protein_id="ANY58984.1"
                     /sec_met_domain="Condensation (E-value: 2.7e-62, bitscore:
                     199.4, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 1.5e-134, bitscore:
                     437.5, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 1.6e-17, bitscore:
                     52.8, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQSILTKEFNSTNSNIFPLSQVQKSAWFLYKLSTQGYADKLALGV
                     RIPSEVDINRLKNAFNILVERHPSLRSAYFQHDKQFVQQVQDKNSAKFEVVDAVGWSEE
                     ELQENLHKSIQLPFNLEAGFVMRMSLFHISPSNYVLLLTVHYIACDWKSLLIFKNELLE
                     LYQEKNIIDLPLLTQSYQDYVEAEIKLFDSSRLQQLQNYWGKQLVGELPLLNLPMSQSR
                     TVMRSYNGASEVLKIAPQLKDKLQVLAESKNITLPVLLLTAFQVLIYRYTGEEDIIVGL
                     LTELRKSELAGVVGNFTNILPLRVAISDNLTFWELCYQVKEVWENAIAYQDYPFPSLVR
                     QLQPDFNPAHPPICQVGFAWEDLNNLNNLKNLSQIEYFELPQQRVEFDVSLDISALENS
                     LTCALKYNTDLFYASTITFMVEHFQNLLQAITFDLDSSISQLSLLSAKEKQQLLFDWNN
                     TQVEYDLSCCLHQLFEIQVEKTPDAVAVKFADKHLTYRELNNRANQLAHYLQSLGVQPE
                     TLVGICIQRCLEMVVGLLGILKAGGAYVPIDPGYPSERITYMLEDSQVSILLTQSNLIV
                     SLPTQPNHTICLDSDWDKISQNSQNNYTNPKSGVKPENLAYVIYTSGSTGKPKGAMNTH
                     QGICNRLLWMQDAYQLTAEDKILQKTPFSFDVSVWEFFWTLITGARLVVAEPGGHRDST
                     YLFNTIIEEGITTLHFVPSMLQVFLETKELEKCQSLKRVICSGEALPLDLQKRFFARLG
                     CELHNLYGPTEAAIDVTFWQCQPNTNLKSVPIGRPIANTQIYILDKHLQPVPAGVVGEL
                     YIGGVGVGRGYLNKVELTGDRFISNPFAFGKMYKTGDLARYLPDGKIDYIGRIDNQVKI
                     RGFRIELGEIENAISQYNFVREAVVVAPSDRLNNKQLIAYIVPHQEELTSKELRDFLKE
                     KLPEFMIPSAFVILDALPLTPSGKVDRRALPKPELSVFSRHESFVSPRNNLEKQLAEIW
                     SEILNISEISVKDNFFEIGGNSLSAVYLMSAIAEKFHRELPLAILFANSTIEELAEILQ
                     NSNQVFANSPLVPIQPKGKKQPFFCIHPAGGHVLCYFNLSRYLGNDQPFYGLQAQGFYG
                     DEEPLTTVEEMASLYVKTIRTFKPEGPYQIGGWSFGGVVAYEVAQQLQRQGQEVSLLAI
                     LDSYVPILLDKAKPIDNTYLVGVLSRVFGGMFGQDNLVSPQEIEHLTIEEKLDYIIDKA
                     RQAKIFPPGVERQNNRRILDVLVGTLKATYSYQRQPYPGKVTIFRAQEKHIMAPDPTMV
                     WVELFSVLAANDIEIYKVPGHHYSFVLEPHVKVLAERLSDRLCK"
     aSDomain        5281..6153
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_mysE_Condensation_LCL.1"
                     /domain_subtype="Condensation_LCL"
                     /evalue="1.30E-67"
                     /label="mysE_Condensation_LCL.1"
                     /locus_tag="mysE"
                     /protein_end="307"
                     /protein_start="16"
                     /score="219.8"
                     /tool="antismash"
                     /translation="FPLSQVQKSAWFLYKLSTQGYADKLALGVRIPSEVDINRLKNAFN
                     ILVERHPSLRSAYFQHDKQFVQQVQDKNSAKFEVVDAVGWSEEELQENLHKSIQLPFNL
                     EAGFVMRMSLFHISPSNYVLLLTVHYIACDWKSLLIFKNELLELYQEKNIIDLPLLTQS
                     YQDYVEAEIKLFDSSRLQQLQNYWGKQLVGELPLLNLPMSQSRTVMRSYNGASEVLKIA
                     PQLKDKLQVLAESKNITLPVLLLTAFQVLIYRYTGEEDIIVGLLTELRKSELAGVVGNF
                     TNILPLRVAI"
     aSModule        5281..9153
                     /complete
                     /domains="nrpspksdomains_mysE_Condensation_LCL.1"
                     /domains="nrpspksdomains_mysE_AMP-binding.1"
                     /domains="nrpspksdomains_mysE_PCP.1"
                     /domains="nrpspksdomains_mysE_Thioesterase.1"
                     /final_module
                     /locus_tags="mysE"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       5356..5454
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_mysE_0001"
                     /evalue="1.10E-09"
                     /label="C2_LCL_024-062"
                     /locus_tag="mysE"
                     /protein_end="74"
                     /protein_start="41"
                     /score="30.7"
                     /tool="antismash"
                     /translation="ALGVRIPSEVDINRLKNAFNILVERHPSLRSAY"
     CDS_motif       6058..6144
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_mysE_0002"
                     /evalue="3.90E-05"
                     /label="C5_LCL_267-296"
                     /locus_tag="mysE"
                     /protein_end="304"
                     /protein_start="275"
                     /score="16.2"
                     /tool="antismash"
                     /translation="DIIVGLLTELRKSELAGVVGNFTNILPLR"
     CDS_motif       6211..6312
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_mysE_0003"
                     /evalue="1.60E-11"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="mysE"
                     /protein_end="360"
                     /protein_start="326"
                     /score="36.5"
                     /tool="antismash"
                     /translation="AIAYQDYPFPSLVRQLQPDFNPAHPPICQVGFAW"
     aSDomain        6649..7848
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_mysE_AMP-binding.1"
                     /evalue="7.50E-124"
                     /label="mysE_AMP-binding.1"
                     /locus_tag="mysE"
                     /protein_end="872"
                     /protein_start="472"
                     /score="405.2"
                     /tool="antismash"
                     /translation="FEIQVEKTPDAVAVKFADKHLTYRELNNRANQLAHYLQSLGVQPE
                     TLVGICIQRCLEMVVGLLGILKAGGAYVPIDPGYPSERITYMLEDSQVSILLTQSNLIV
                     SLPTQPNHTICLDSDWDKISQNSQNNYTNPKSGVKPENLAYVIYTSGSTGKPKGAMNTH
                     QGICNRLLWMQDAYQLTAEDKILQKTPFSFDVSVWEFFWTLITGARLVVAEPGGHRDST
                     YLFNTIIEEGITTLHFVPSMLQVFLETKELEKCQSLKRVICSGEALPLDLQKRFFARLG
                     CELHNLYGPTEAAIDVTFWQCQPNTNLKSVPIGRPIANTQIYILDKHLQPVPAGVVGEL
                     YIGGVGVGRGYLNKVELTGDRFISNPFAFGKMYKTGDLARYLPDGKIDYIGRIDNQVKI
                     R"
     CDS_motif       6835..6876
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_mysE_0004"
                     /evalue="5.30E-06"
                     /label="NRPS-A_a2"
                     /locus_tag="mysE"
                     /protein_end="548"
                     /protein_start="534"
                     /score="19.5"
                     /tool="antismash"
                     /translation="LGILKAGGAYVPID"
     CDS_motif       7078..7137
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_mysE_0005"
                     /evalue="5.20E-10"
                     /label="NRPS-A_a3"
                     /locus_tag="mysE"
                     /protein_end="635"
                     /protein_start="615"
                     /score="31.2"
                     /tool="antismash"
                     /translation="AYVIYTSGSTGKPKGAMNTH"
     CDS_motif       7645..7734
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_mysE_0006"
                     /evalue="2.50E-14"
                     /label="NRPS-A_a6"
                     /locus_tag="mysE"
                     /protein_end="834"
                     /protein_start="804"
                     /score="45.3"
                     /tool="antismash"
                     /translation="PAGVVGELYIGGVGVGRGYLNKVELTGDRF"
     CDS_motif       7813..7878
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_mysE_0007"
                     /evalue="1.80E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="mysE"
                     /protein_end="882"
                     /protein_start="860"
                     /score="39.0"
                     /tool="antismash"
                     /translation="YIGRIDNQVKIRGFRIELGEIE"
     aSDomain        8176..8382
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_mysE_PCP.1"
                     /evalue="2.40E-22"
                     /label="mysE_PCP.1"
                     /locus_tag="mysE"
                     /protein_end="1050"
                     /protein_start="981"
                     /score="70.8"
                     /tool="antismash"
                     /translation="EKQLAEIWSEILNISEISVKDNFFEIGGNSLSAVYLMSAIAEKFH
                     RELPLAILFANSTIEELAEILQNS"
     CDS_motif       8440..8469
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_mysE_0008"
                     /evalue="6.00E-03"
                     /label="NRPS-beforete1"
                     /locus_tag="mysE"
                     /protein_end="1079"
                     /protein_start="1069"
                     /score="9.7"
                     /tool="antismash"
                     /translation="PFFCIHPAGG"
     aSDomain        8440..9153
                     /aSDomain="Thioesterase"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_mysE_Thioesterase.1"
                     /evalue="1.10E-36"
                     /label="mysE_Thioesterase.1"
                     /locus_tag="mysE"
                     /protein_end="1307"
                     /protein_start="1069"
                     /score="119.2"
                     /tool="antismash"
                     /translation="PFFCIHPAGGHVLCYFNLSRYLGNDQPFYGLQAQGFYGDEEPLTT
                     VEEMASLYVKTIRTFKPEGPYQIGGWSFGGVVAYEVAQQLQRQGQEVSLLAILDSYVPI
                     LLDKAKPIDNTYLVGVLSRVFGGMFGQDNLVSPQEIEHLTIEEKLDYIIDKARQAKIFP
                     PGVERQNNRRILDVLVGTLKATYSYQRQPYPGKVTIFRAQEKHIMAPDPTMVWVELFSV
                     LAANDIEIYKVPGHHY"
     CDS_motif       8623..8694
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_mysE_0009"
                     /evalue="6.20E-15"
                     /label="NRPS-te1"
                     /locus_tag="mysE"
                     /protein_end="1154"
                     /protein_start="1130"
                     /score="47.4"
                     /tool="antismash"
                     /translation="PEGPYQIGGWSFGGVVAYEVAQQL"
     gene            complement(9286..10167)
                     /locus_tag="UCFS10_04340"
     CDS             complement(9286..10167)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001:short-chain dehydrogenase/reductase SDR (Score:
                     195.1; E-value: 2.3e-59)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="UCFS10_04340"
                     /product="short-chain dehydrogenase"
                     /protein_id="ANY58988.1"
                     /transl_table=11
                     /translation="MPVDRRLEGKVAIITGAGAGIGEAIAHKFAKEGASVIVNGLPDDP
                     IEDVVQAIRHYGGKAIAYLGDVSQEFHAQNCVQTAINAYGKLDILINNAGVFLATAETQ
                     DYPVDVFDDTIRNNMRSAFLMTKYALPHLQKTRGNIVSAGSEAGFNGLAFNTTYGGTKG
                     WMHSFMLGVAVEQAKYGIRANCVCPGAIDTAWTHKETGPMDAKMEKSLIEGTPLARRGT
                     PEEMANVYAFIASDEASYVTGALWLADGGVTVAKGSAGSDTPFWMRFAPKGELRLDHSK
                     EGLENKQTQTLK"
     gene            10423..10914
                     /locus_tag="UCFS10_04341"
     CDS             10423..10914
                     /codon_start=1
                     /locus_tag="UCFS10_04341"
                     /product="orange carotenoid protein"
                     /protein_id="ANY58989.1"
                     /transl_table=11
                     /translation="MTFTQAGDPTIREHVQAFQNLDVDDQLALFWFIYKEMGKSITPAA
                     PGASTVSPQIAEGLFNQVKELSHEEQLQLQRDLINRVDNQLTREYGSLGDTTKLLFWYL
                     LAQGMDNATIIPMPANYQLSSESENLFNKIKEIPFEEQITLFRDYVSPMGAEAKAGAEI
                     "
ORIGIN
        1 acgttatggt gaggatgcac taaagagcga ttgttatggc agatcaagaa cagatagatt
       61 tagaagctca actaaatcgt gtagatccag ataaggaagc gcaacctgag gatttgtcag
      121 agcaaatttc cgacttaccg caggcaatta ccgagtctta tggtactggt gtgaaagaca
      181 tgccaggata taacatcggt gggcgaagca tgcgcgatga aagacagaat tacacagaaa
      241 ctagccccga attaactggg ggcgatgttg atgcttattg gcaagacgcc gatgcagtag
      301 gcgatgaagc cgttggtgga acagttgcga ctcccgatca aaatgtaact gaggaaatag
      361 aagcagctgt tggtctggaa atggacgatc gcgcgtttct cagaactaac gatattttag
      421 aggaacgcga tgaccgacgc tgggagttag atccaacatc ttccgaagat tatcaagaac
      481 gcagagacta atgctggggt ggcggcggct tcataaaccc ggtttcttta aaaaatcggg
      541 tttctccatc ttcgcgaatc atttaggatt actatcacga atggagatat ttaaagaaat
      601 cgaaacatat ttaaatctta taatttattt aaagatatat aagtaataat cattataaag
      661 tttgataaat aggttcccta atagagttaa gctaaatttt gtggctaaat tcaataaaat
      721 taatcttaaa tactaatttt ttgaatattg cctgcgttaa tgctttgact gctatcaaag
      781 taattaagct tatgaaattt ggagtatttc tatccaaact ataagtaacg catgacaaat
      841 tatctctgtt tctaacgata taaggttcaa gacaaatgct aaaaaacaac gaactgaata
      901 tcaaccggag ttctaatcta aggtggaaac taggagacca agataaccaa ttctccacag
      961 gacaagtttt gccgtttaaa acagcaggta aaccgctata aataaaagcg gctggtttga
     1021 aattaaataa attgccatca ttgatgctga gttgctgtca aatgtatcaa gataggtttt
     1081 ttgcctattt aataagttgc atttgagggt atattagtgt ttttgtgggc tgctaggcta
     1141 tctatatcat gcagcaagaa aaaagattca aaagatagtc tatttttcag caatttcttt
     1201 tcaggaaata tatccagtta atcagtctaa agaggtatgc atatctctgt tgattgatag
     1261 attgttttat cctgttttca atcagaaaat gggaaaaatt tcttttagct gcgattttca
     1321 gctaaaagct caaatttgcc attaattttg ttagttttgg aataaaggac agaagagatg
     1381 agtgtacctt atgcaacttt tgcggcgacg gaaacggcat ttcacgtaac aggttatgaa
     1441 aaaattgaat ttagtctggt ttatgtaaat ggcgcgtttg atattaaaaa cactgagata
     1501 gctgatagct atcaaaattt tgggcgttgt ttgttagtag ttgatgcaaa tgtcaatcgt
     1561 ttatatggcg agcaaattag gtcttatttt caattttata atattaaatt gacagttttt
     1621 cctatcacta ttagcgagcc agccaagagt ctgactacat ttgaagaagt tatagacgct
     1681 tttgcggact ttggtttaat tcgcaaagag ccagttttag tagttggtgg tggtttagtt
     1741 acagatgtgg ttggttttgc ctgtgcggct tatcgtcgca gtacgaatta tattcggatt
     1801 ccaacaactt taattggttt aattgatgcg ggtgttgcaa tcaaagtagc agtgaatcat
     1861 aagaagttaa aaaatcgttt gggtgcttat catgctcccc aaaaagtaat tctagatttt
     1921 tcctttttag aaactctgcc aattgctcaa attcgtaacg ggatggcgga attggtaaaa
     1981 attgccgtgg tggcgaatgc agaggttttt gaactgttgt atgagtacgg tcatgaatta
     2041 ctgcatacac actttggcta tgtgaatggt tccaaagaat tgcaagaaat tgctcatcgt
     2101 ttaaattatg cagctatcaa aaccatgctg gaattggaaa cgccaaacct gcgtgaattg
     2161 gatttagata gggtaattgc ttacggtcat acttggagtc cgacattgga actcgcacca
     2221 caggttcctt tatatcacgg tcatgcagtt aatattgata tggcgttgtc tgcaactatt
     2281 gcccacagac ggggttatat taatttggaa caacgcgatc gcattctcaa tctcatgagt
     2341 cgcattggtt tggctcttga ccatccttta cttgatagtg atttgttgtg gtacgctact
     2401 caatctatca cccaaacaag agatggtaaa ttacgcgcag ctatgccaaa accaattggt
     2461 gagtgctttt ttgtgaatga tttgactcgc gaagaattgg atgaagcttt ggcggaacat
     2521 aaacttctgt gtgctgaact tcccggtggt ggtgaaggaa tcgacgctta tattggtact
     2581 gaagaatcgg aattaatcaa agcgtaatat catgcatgtg aatacttgta ctatatggtt
     2641 aatggttaat tgttaattgt taatggttaa tagtcaaaat ccatgaatca tgaaccatct
     2701 aactacaagt aattaagcgg acatgatgta aaaccgtttt ctttcaaact tgcaacagat
     2761 aatttggatt ggtttgcaca atccaaaatc ctttcatcca aaatccaaaa tggtaagacc
     2821 gtgacaagta ttgtagaaat cagtaccgct agacccgtaa caccccacgg tattttagta
     2881 gaagagttgc gaaaaactct agaaatggcg gaagctgcaa gcattccagc agaaattttg
     2941 gcaaccctgc gctctacgta tcaattagcg gcaggattag acccatatat cagtgaatgt
     3001 acgacgaccg aatcgtcagc gctgacagca ttggcgcgga aaactagcgc ggaagattgg
     3061 cgaaagcgct ttggtgatgg tgaaacggtg cgtcagttag aacaagagat gctttcggga
     3121 cacctggaag gacagacatt aaaaatgttt gttcgcatga ctaaagccaa gcgggttttg
     3181 gaagtgggga tgtttacagg atattcagcg ctagcaatgg cggaagcgtt acccgatgat
     3241 gggaagttgg tggcttgtga ggttgatgct tatgtcgcca actttgccca aaactgcttt
     3301 gctgattcgc cccacggcga taaaattacc gtggaagtag cacctgcttt ggaaacactt
     3361 cataaattgg ctgcagctgg tgaatctttt gatttgatat tcatcgatgc agataaaaag
     3421 gagtatgtgg agtacttcca aactattttg gatagtaact tgcttgcacc cgacggttta
     3481 atctgcgttg ataatacttt attgcaagga cagccttacc ttccaccaga gaaacggacg
     3541 gcgaatggag aagcgatcgc cacgtttaat tccattgtcg cccaagattc tcgtgttgag
     3601 caagtgctgt taccagtgcg agatggttta actttgatta gaaggttgga ttaaatggca
     3661 caatctatac cttttgattc tgcatcgcca acgccgcaag tttcctgggg agtaaggata
     3721 tctgcgttat ggaaaaccgt aggtacactg ttgttgttgt ttttagcatt accagtaaat
     3781 gcctcgattg ttttgatatc tttgctgtgg ggtattttca gcaaaccctt tgagaaacga
     3841 gttgtagccg ccgcaccgaa aaatatctta attagcggcg gtaaaatgac caaggctttg
     3901 caacttgcgc gatcgttcca cgccgcagga caccgagtag tattggtgga aagtcataaa
     3961 tattggttaa ccggacatca attttccaat gctgtgagtg ttttttatac tgtaagtcca
     4021 ccggaaaaag acccggaagg ctatacacag caactattag acatcgtaaa aaaagaacgc
     4081 attgatgttt acgtaccagt gtgcagtcct gtcgccagtt actacgattc cttggtgaaa
     4141 cctgcgcttt ctcagcattg tgaggtgttt cactgcgatg cagagataac gcagatgttg
     4201 gatgataaat acgcattttc cgaaaaagct agaagttttg gtttatctgt tcccaagtcg
     4261 tttaaaatta cgaatcccga acaggttatt aacttcgatt tttctcagga aaagcgcaaa
     4321 tacatcctta aaagtatccc ttatgattcg gttcggcggt tgaatttaac taaacttccc
     4381 tgcgacactc ccgaagaaac agcagcgttt gtgcgaagtt tacccatcag tccggagaaa
     4441 ccttggatta tgcaagagtt cataccgggt aaggagtttt gcacccacag tactgttaga
     4501 aatggggaat taagattgca ttgctgttgc gaatcttcgg cgtttcaggt gaattacgaa
     4561 aatgtaaata atccgcaaat tttggaatgg gtaaaacatt tcatcaaaga aatgggtatc
     4621 accggacaag tttcctttga ttttattcaa accgaagacg gaacagttta cgcgattgaa
     4681 tgcaaccccc gcactcattc tgctatcacg atgttttaca atcatcctgg tgttgcggat
     4741 gcttatttgg gtaaaattcc tttacctgaa cctttacaac ctcttgctga tagtaagcca
     4801 acgtattggc tttatcatga aatttggcgg ttgacgggaa ttcgttcttt gtcgcaattt
     4861 tggacttggt taaagaattt gatgcgcggt aaagatgcaa tttatcagtt gaatgaccct
     4921 ttaccttttt tgactgttcc tcattggcaa attactttat tattgttgca aaatttgcga
     4981 caattacgag gttgggtgaa gatagatttc aatattggta agttggtgga gttgggtgga
     5041 gattaggttt tatgtagaaa gtaatagatt ttgtaggttg ggtgtagctt tcttcggaac
     5101 ccaacatcaa ccttgatttg ttgggttcca ctttgttcca acgccacttg cgcggctgcc
     5161 gggaaacccg tccaacgcag tggcttccca acctacaact agaaaaatgt tgaaggaaat
     5221 aaactaaaaa ttatgcaaag tatattaact aaagaattta actcaacaaa ctctaatata
     5281 ttccccctgt cgcaagttca aaaatcggct tggtttctct acaagttatc gacccaagga
     5341 tatgctgata agcttgcttt aggagttcgc attccttcag aagtagatat aaatagatta
     5401 aaaaatgcct ttaatatcct tgtggaacgt cacccaagtt tgcgaagtgc ttactttcaa
     5461 catgataagc aatttgttca gcaagtacag gataaaaatt ctgcaaaatt tgaagttgtc
     5521 gatgctgtgg ggtggagtga agaagaactt caagaaaatt tgcacaagtc gattcagctt
     5581 ccttttaact tagaagctgg ttttgtgatg cgaatgagtt tatttcatat ttcgcctagc
     5641 aattatgtct tgttgctaac ggtgcattac atcgcttgcg attggaagtc tttgctgata
     5701 tttaaaaatg aattactgga attatatcaa gaaaaaaata ttattgactt acccctgtta
     5761 actcaatcat atcaagatta tgtagaggca gaaatcaagc tttttgatag ttcgagatta
     5821 cagcaactgc aaaattattg gggaaaacag ttagtaggtg aattaccgct gttaaattta
     5881 cccatgagtc aatctcgtac ggttatgcga agctataacg gtgcttctga agtattgaaa
     5941 atagcaccgc aattaaagga taaattacaa gttttagccg aatcgaaaaa tattacttta
     6001 cctgtattac ttctgactgc attccaagtc ttaatttatc gctatacggg tgaagaagat
     6061 attatagttg gtttgctaac ggaactcaga aaatcagagc tagctggcgt tgttgggaac
     6121 ttcactaata tattaccctt gcgagtagct atttctgata atctgacatt ttgggaatta
     6181 tgttatcagg taaaagaggt atgggaaaat gcgatcgctt atcaagatta tcccttccct
     6241 tccctagtcc gacagctaca accagacttt aaccccgcac atccccccat ttgtcaggtt
     6301 ggttttgcat gggaagacct gaataatctc aataatctca aaaatctttc ccaaatcgaa
     6361 tattttgaac taccacagca gcgagttgaa tttgatgtca gccttgatat ttccgctttg
     6421 gaaaattcgc tgacatgtgc gttgaaatat aataccgatt tattttacgc ttctactatt
     6481 acttttatgg tagaacattt ccaaaattta ttgcaagcaa ttacatttga tttggattcc
     6541 tctatatctc aactttcctt gttaagtgca aaggaaaaac aacaactatt atttgactgg
     6601 aataatactc aagttgaata tgacttatca tgctgtttgc atcaattatt tgaaattcag
     6661 gtagagaaaa cacctgatgc tgttgcagtc aaattcgcag ataaacacct cacctacaga
     6721 gaattaaaca accgcgccaa tcaactcgca cattacttgc aatctttagg cgtccaacca
     6781 gaaacgctgg tgggaatttg tattcaacgt tgcctagaaa tggtagtggg actgttagga
     6841 attctcaaag ctggtggtgc atatgtacca attgacccag gatatccctc cgaacgcatt
     6901 acatacatgc ttgaagattc ccaggtgtca attttactaa cccaaagtaa cctgattgtc
     6961 agtttaccaa cgcagccaaa tcatacaatc tgcttggata gcgattggga taaaatttcc
     7021 caaaattctc aaaataatta taccaatccc aaatctggag tcaagccaga aaatttagct
     7081 tatgtaattt acacctccgg ttccacagga aaacccaagg gtgcgatgaa cacccatcaa
     7141 ggtatctgca atcgcttgct gtggatgcaa gatgcatacc aactcaccgc tgaagataaa
     7201 attctccaga aaacaccttt tagttttgat gtttcagtgt gggaattttt ctggacattg
     7261 ataacaggtg cgcgtttagt agttgcggaa ccaggtggac accgtgatag tacttacctg
     7321 tttaacacca ttattgaaga gggaattact acactgcact ttgttccctc catgcttcag
     7381 gtatttttgg aaactaagga attagaaaaa tgtcaatcgc tcaaacgagt gatttgtagc
     7441 ggtgaagctt tacctctaga tttacaaaag cgtttctttg cacgtctggg atgcgaatta
     7501 cacaacctct acggccctac cgaagccgct attgatgtca cattttggca gtgtcaacca
     7561 aatactaact taaaaagtgt acccattggt cgtcccattg ccaacaccca aatttacata
     7621 ctcgacaaac atttacaacc cgtccccgca ggtgttgttg gcgaactata tattggtggt
     7681 gtgggagttg gaagagggta tctaaacaaa gttgaattaa caggcgatcg ctttatttcc
     7741 aatcctttcg ctttcggtaa aatgtacaaa actggcgact tagctcgcta tctacctgat
     7801 ggtaagattg attacatcgg tagaattgat aatcaagtaa aaattcgcgg ctttcgtatc
     7861 gagctaggtg aaatagagaa cgctataagt cagtataatt tcgtaagaga agcagttgtt
     7921 gttgctccta gcgatcgcct aaacaacaaa caactaattg cttatattgt cccccatcaa
     7981 gaagaactta catctaagga attgcgggat tttctcaaag aaaaacttcc cgaatttatg
     8041 ataccgtcag cgtttgtaat cttagatgca ttacccttaa ctccgagtgg aaaagttgat
     8101 agacgtgcgc ttcccaaacc ggaattatct gtttttagcc gtcatgaatc ttttgtttct
     8161 ccccgcaata acttagaaaa acaactagcg gaaatttggt cagaaatttt aaatatttca
     8221 gaaatcagcg tcaaagataa cttttttgaa attggcggaa attcgctttc agcagtttat
     8281 ttaatgagtg cgatcgccga aaaatttcat cgagaattac ccctagcgat tctatttgca
     8341 aatagcacaa ttgaagagtt agctgaaatt ctgcaaaact caaatcaagt ttttgctaac
     8401 tctccattag ttccaattca gccaaaaggt aaaaaacaac ctttcttctg cattcatccc
     8461 gcaggcggtc acgttttgtg ctactttaat ttatcgcgtt atttgggtaa tgaccaacca
     8521 ttttatggtt tacaagcaca aggattttat ggcgatgaag aaccgttaac aacagttgaa
     8581 gaaatggcga gtctctacgt caaaaccatc cgaaccttta aaccagaagg tccatatcaa
     8641 attggcggat ggtcttttgg tggagttgtc gcttatgaag ttgctcaaca attgcagcga
     8701 cagggacaag aagtatcgct gttagcaata ttagattctt acgtaccaat tttattagat
     8761 aaagccaaac caatagataa tacttattta gttggcgtgc tttctcgcgt ttttggcgga
     8821 atgtttggtc aagataactt agtttcacct caagaaattg agcatttaac tatagaagaa
     8881 aaacttgatt atatcatcga caaagccaga caagccaaga tatttcctcc tggtgtggaa
     8941 cgtcaaaaca accgtcgaat tttagacgtt ttggtgggaa ctctcaaagc cacttattct
     9001 tatcaacgtc aaccttatcc aggaaaagta actatttttc gggcacagga aaaacatatc
     9061 atggctcccg acccaacaat ggtatgggta gaattatttt ccgtacttgc agcaaatgat
     9121 attgagattt ataaagttcc cggtcatcat tactcatttg tgctggaacc acacgttaaa
     9181 gttttagcgg aacgtttaag cgatcgcttg tgtaaataaa caaaactata ctgcaatggg
     9241 aaaagggaaa atattaaact ttttttccct ttgccccgct ttcgtttact taagcgtctg
     9301 cgtttgtttg ttctctaacc cttctttaga atggtcgagg cgtaattcac ctttaggtgc
     9361 aaagcgcatc caaaaaggag tgtctgaacc tgcagaccct ttagcaacgg tgactccacc
     9421 atcagccagc cacaatgcgc ccgtaacgta actggcttca tcggaagcta taaaagcgta
     9481 gacattcgcc atttcttccg gtgtaccgcg tcgcgccaat ggcgtacctt caatcaaact
     9541 tttttccatc ttggcgtcca tcggtccagt ttccttatgg gtccacgcag tatcaatcgc
     9601 accgggacag acacagttag cacggatacc atacttggct tgttcgaccg caacaccaag
     9661 catgaatgaa tgcatccagc ctttggtacc gccgtatgtt gtgttgaatg ccaatccgtt
     9721 gaaacccgct tcagaacccg cagagacaat gttaccccgc gttttctgca agtgcggaag
     9781 tgcgtatttg gtcatcaaaa acgccgatcg catgttattg cggattgtgt catcgaatac
     9841 atcaactggg taatcttgag tttcggctgt agcaagaaat actccagcat tattaatcaa
     9901 gatgtctaat ttgccgtaag cattaattgc ggtttggacg cagttttgag cgtggaattc
     9961 ttgagaaaca tctcctaagt aggcgatcgc tttaccgcca taatgtctaa ttgcctgtac
    10021 tacatcttca atcggatcgt caggtaatcc gttaacaata actgatgcac cttctttggc
    10081 aaatttgtga gcgatcgctt ctccaatacc agcgccagca ccagtgataa ttgcaacttt
    10141 accttctaaa cgtctatcca ccggaatacc tcctagaagt aaaaatctgt tagtagatag
    10201 ttgttagggg tttgttggtg gttgattgtt aatggttctt tgggaagaaa accaccaacc
    10261 aacacccaac acccaacccc caacaaccaa cacccaacac ccaacaactt ccaattgttc
    10321 ttctctttga ttgagataca tctgtcttca gtagggttaa acgccggata cagctttgat
    10381 acctttgctt tagtgcatca ttaattgcaa tcggagtttg ttatgacatt tactcaagct
    10441 ggcgacccaa ctattcgcga acatgttcaa gcttttcaaa atttggatgt agatgaccaa
    10501 ctcgctttgt tttggttcat ctataaagaa atgggcaaat caatcacacc agcagccccc
    10561 ggtgctagca ctgtttctcc acaaattgct gaaggtttgt tcaaccaagt taaggaatta
    10621 tctcacgaag aacaactaca acttcagcgc gatttgatta atcgtgtgga taaccaactt
    10681 acccgtgaat atggctcttt gggtgacact accaagctgc tattttggta tctgttagct
    10741 caaggcatgg acaatgctac tatcattcct atgcctgcca attatcagct ttcctcagaa
    10801 tcggaaaacc tgttcaacaa aatcaaagaa attccttttg aagaacagat aactcttttc
    10861 cgcgattatg tttcgccgat gggtgctgaa gcaaaagcag gggctgaaat ttagttgaaa
    10921 tttaattaaa atttcaatct tagtaatttt gcttttcaag agtttctcat gcattaattg
    10981 cgagaag
//
