LOCUS       BGC0001655              5572 bp    DNA     linear   CON 16-MAR-2022
DEFINITION  Nocardiopsis alba DSM 43377 contig_10, whole genome shotgun
            sequence.
ACCESSION   BGC0001655
VERSION     BGC0001655
KEYWORDS    .
SOURCE      Nocardiopsis alba DSM 43377
  ORGANISM  Nocardiopsis alba DSM 43377
            Bacteria; Actinobacteria; Streptosporangiales; Nocardiopsaceae;
            Nocardiopsis.
COMMENT     REFSEQ INFORMATION: The reference sequence is identical to
            ANAC01000010.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2022-11-08 03:16:12
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 360088
            Orig. end    :: 365660
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     subregion       1..5572
                     /aStool="mibig"
                     /contig_edge="False"
                     /label="RiPP"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..5572
                     /contig_edge="False"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            379..519
                     /locus_tag="D451_RS27835"
     CDS             379..519
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014911574.1"
                     /locus_tag="D451_RS27835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="lasso RiPP family leader peptide-containing
                     protein"
                     /protein_id="WP_014911574.1"
                     /transl_table=11
                     /translation="MDEEKIGNEISAYETPTVTEMGAFSEVTLGRPNWGFENDWSCVRV
                     C"
     gene            657..2501
                     /locus_tag="D451_RS0108905"
     CDS             657..2501
                     /GO_process="GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Asn_synthase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1177:asparagine synthase (glutamine-hydrolyzing)
                     (Score: 114.3; E-value: 1e-34)"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017534152.1"
                     /locus_tag="D451_RS0108905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="asparagine synthase-related protein"
                     /protein_id="WP_017534152.1"
                     /sec_met_domain="Asn_synthase (E-value: 5.4e-36, bitscore:
                     113.8, seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFIVLPSTGDVAGVSIERLARTHLQVVHRASGRPWIIGDWDEGD
                     VYKARIGNRRVVLLGRFLMDEPALLRVLDSAESLSDLSRVTEEIPGSYHLVAELDEGVR
                     IQGTLSSGRQIFYSNRGSPVLAASDPALLLPFVGREWDRSILASRLIAPWPPWPVSEIP
                     VWKGVSALAPGHFLQVDSRTGTARQVRWWSPEEPHLTLERGAELLASAVHDAVRSRGRG
                     RTTVSADLSGGLDSTGLCFVAAATGVPLVTTRWQATDVADEDEAWARKAERRLPEARHL
                     TFDRRSAPGWFTELLPRRPGVHDHEGPFPWIRTRGRLAHAAEAVADEGSRTHLTGHGAD
                     ELFFATPLNLHSLARTHPAKVPAHVLGYQALYRWRTAPTLRGLADSSTFSAWLGKVTGS
                     LRTPLREFSTAPDFGWGLSGRMARWVAPEAVEAASEALRRDDVLSRGPLSRYRAQHASW
                     QDARLCGDTLRKVNHITRAHGVSWEAPYLDDRVMRIALSVRFDQWSMPDRYKPLLVEAL
                     RGTAPPEILERSTKSEYSAEAYTGLRRSREAVAELCDGMRLEEHGLVDAEAFGGTVFNP
                     PSSSFGIIPVINTLACESWLRSIEDGSREKDLVSILEG"
     gene            2504..2761
                     /locus_tag="D451_RS0108910"
     CDS             2504..2761
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RRE-containing: Stand_Alone_Lasso_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014908438.1"
                     /locus_tag="D451_RS0108910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="lasso peptide biosynthesis PqqD family chaperone"
                     /protein_id="WP_014908438.1"
                     /sec_met_domain="PF05402 (E-value: 3.2e-17, bitscore: 51.6,
                     seeds: 461, tool: rule-based-clusters)"
                     /sec_met_domain="Stand_Alone_Lasso_RRE (E-value: 1.6e-23,
                     bitscore: 71.6, seeds: 25, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEFIDDTSIISTETEDGIVLLDQRKGVYWHLNGTAAEILRSLRED
                     GDRDVAVAEIRDRYGIDESRARNDVEDLIRSLESVGVLRR"
     gene            2857..3192
                     /locus_tag="D451_RS0108915"
     CDS             2857..3192
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: PF13471"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017622998.1"
                     /locus_tag="D451_RS0108915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="WP_234305725.1"
                     /sec_met_domain="PF13471 (E-value: 1e-25, bitscore: 79.0,
                     seeds: 84, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSPGRIRSVLAFLAGSAGPAGFAEALAARNAVVHVSRRCAGPGCL
                     QRSIATFLLCRIRGTVPEWCTGVRVSPFRAHAWVEVDGSPVGELGEVRHFHKVMTVRPV
                     IVVRDDD"
     gene            3182..3775
                     /locus_tag="D451_RS0108920"
     CDS             3182..3775
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014913299.1"
                     /locus_tag="D451_RS0108920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_020379946.1"
                     /transl_table=11
                     /translation="MMTEIVALAAQYALFLIFLAAWVGKIKGPGRFSGFATVISRLTGW
                     SPPTARAVGVTVVAGEVLACLLLISPLTAAAGAVVALTLLTAFTGVAVKALWSGIRSEC
                     ACFGGSDSAIGYAMIVRNLLLIALALPPLVVRADLADLSATGVVAVALGALAIALLLIG
                     FYDPLARRATAAIIATRRATRPRAGTAGGGDDVG"
     gene            3765..4697
                     /locus_tag="D451_RS0108925"
     CDS             3765..4697
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 191.7; E-value:
                     2.7e-58)"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017534155.1"
                     /locus_tag="D451_RS0108925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_202421642.1"
                     /transl_table=11
                     /translation="MSGDSIEVSKVGKVFGSVRALNDVSLSVSPGGVLGLLGHNGAGKS
                     TLINILSGVLEPTTGTATVAGFDVVSQASRVRRRIGVAGQYVALDEQISGRDNLVLIAR
                     LLGASRKEARERTHELLERFGMTESADRAVRTYSGGMRRRIDLAASIVGGPEVLFLDEP
                     TAGLDPESRAELWAIVHRLTEEGVTVLLTTQYLEEADRLSDQVVLLSRGHVVASGSPSE
                     LKRGVGRRKASLGFNERERFSAARDEVRRMGLVADIGEANFDIDITIASPRDLGAVIHA
                     LEERNLGIDRLRLFEPSLEDVYLAHVSTR"
     gene            4730..5572
                     /locus_tag="D451_RS0108930"
     CDS             4730..5572
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1065:ABC-2 type
                     transporter (Score: 167.2; E-value: 5.9e-51)"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014911386.1"
                     /locus_tag="D451_RS0108930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter permease"
                     /protein_id="WP_036567929.1"
                     /transl_table=11
                     /translation="MTIDARARRSTVPWKSVSWRGCSVPLQIHILTTRALRALWMDPKL
                     VLVSVASPLVMLLVLSQVFTSISETRSFPGGVDYIQFLLPAIMIMGVLQSGMGSGAALG
                     QEIKNGFVHRMRTLPVWPGSVLLGRSASDLVRSLIQLSALSTAAHLFFSFHAPGGAVGM
                     AVAMLIALMTGNCLGWVFIAMACWFRNAEVMQTFSGLAMFALMFGTNAFVPLDGLPDWL
                     RSLSHVNPMTYAITAARGWILGDPDMSATLYAVIGSAVIALPCALLAVKGFARPLGTN"
ORIGIN
        1 ctctgggatc ttcacccata agctccttca tggtccagcc attctggaag cgatgtcgca
       61 cttgagaacg gccgaataga gccagaatga ttattcgacg gtcccccttg tccagatccg
      121 gccaaagcac cacctccacc gacatcgatc tcactgaaag atcagaccag aaccgcatcc
      181 acctgcgatg attcccatgg atccccatga acgcaccaca tcagagaatc gtactttaat
      241 ctgcgaaatc aactcaaaaa gcatttgtcg caccgaccga cgcactcttg accctcgaac
      301 cggggcgcgg ttaacgtctc tttcatcgcc ggggatatct ccgccccgtc gagaccctcc
      361 gagaaaccgg gagcgacaat ggacgaagaa aagatcggca acgaaatctc cgcctacgag
      421 actccgaccg tcaccgagat gggcgcgttc tccgaggtca ccctcggccg ccccaactgg
      481 ggattcgaga acgactggtc ctgcgtccgg gtctgctgac ccgatctcgg aaccggccac
      541 cgcccggaac gaccgaacgg gcactcgggg tcgcgccggc gccgctcggg cgtcggcgcg
      601 ctcatggcct gaggacgagg aatacagggc gaactcgaaa ggcctctcgg agaaaaatga
      661 agttcatcgt tcttccctcc acgggcgacg tggccggagt gtcgatcgag cgtctggcgc
      721 ggacacacct ccaagtcgtt catcgcgcgt ccgggaggcc gtggatcatc ggtgactggg
      781 acgagggcga cgtctacaag gcgaggatcg ggaacaggag ggtcgtcctc ttgggacggt
      841 tcctcatgga cgagcccgcc cttctgcgcg tcctcgactc ggccgagagc ctctccgacc
      901 tctcccgggt cacggaggag atcccgggaa gctaccacct cgtggccgag ctggacgagg
      961 gcgtccggat ccaggggaca ctctcctccg gccgccagat cttctactcg aaccggggct
     1021 cccccgtcct ggccgccagc gaccccgccc tgctgctccc gttcgtcgga cgcgagtggg
     1081 accgctcgat cctggcctca cgactcatcg cgccctggcc gccctggccg gtgagcgaga
     1141 tcccggtctg gaagggggtc tccgccctcg ccccgggcca cttcctccag gtcgactcgc
     1201 ggaccggtac cgcgcgccag gtccgctggt ggtcgcccga ggaaccccac ctgacgttgg
     1261 agcggggggc cgagctcctg gcgtccgccg ttcacgacgc cgtgcgcagc cgcggccgag
     1321 gacggaccac ggtgagcgcc gacctctccg gaggtctgga ctccaccggt ctctgtttcg
     1381 tcgcagccgc cacgggcgta cccctcgtca ccacgaggtg gcaggcgacg gacgtggccg
     1441 acgaggacga ggcatgggct cgcaaggcgg agcggcgact ccccgaggcc cggcacctga
     1501 ccttcgaccg acgctccgcc cccggctggt tcacggagct gctcccccgg cgccccggcg
     1561 tacacgacca cgagggcccc tttccctgga tccgcacccg cggccggctc gcccacgcgg
     1621 ccgaggccgt ggcggatgag gggtcccgga cccacctgac cggccatggc gcggacgagc
     1681 tcttcttcgc cacaccgctc aacctccact ccctggcccg aacacatccg gccaaggtgc
     1741 cggcccacgt tctcggctac caggccctgt accgctggag gaccgctccg acgctcagag
     1801 ggctggccga ttcgagcacg ttctccgcct ggttggggaa ggtcacgggc tccctgcgca
     1861 ccccgctgcg ggagttctcg accgcacccg acttcggatg gggcctctcg ggccggatgg
     1921 cccggtgggt cgccccggag gccgtcgagg ccgcctccga ggcgttgagg cgcgacgacg
     1981 tcctctcccg gggccctctc tcccgttacc gggcgcagca cgcctcctgg caggacgcgc
     2041 gtctgtgcgg agacacgctc cggaaggtga accacatcac ccgcgcgcac ggggtctcct
     2101 gggaggcccc ctatctggac gatcgggtca tgaggatcgc tctgagcgtc cgcttcgacc
     2161 aatggtccat gccggaccgc tacaaacccc tcctcgtcga ggcactgcgc ggcacggcac
     2221 cgccggagat cctcgaacgc tccacgaagt ccgagtacag cgcggaggcc tacaccggac
     2281 tccggaggag tcgcgaggcc gtcgccgaac tctgcgacgg aatgagactc gaagaacacg
     2341 gcctcgtcga cgccgaggcc ttcggcggga cggtcttcaa ccccccgtcc tcctccttcg
     2401 ggatcatccc cgtcatcaac acactggcct gcgagtcctg gctccggagc atcgaggacg
     2461 gaagcaggga gaaggacctc gtatcgatcc tggaaggctg aggatggaat tcatcgacga
     2521 cacgtccatc atctccaccg agaccgagga cgggatcgtt cttctggacc agaggaaggg
     2581 cgtgtactgg cacctgaacg gtaccgccgc ggagatcctg cgctccctga gagaggacgg
     2641 ggatcgggac gtcgccgtcg ccgagatccg ggacaggtac gggatagacg aatcccgggc
     2701 caggaacgac gtcgaggacc tgatccggtc gctcgaatcc gtgggggtgc tgcggcgatg
     2761 acggtacccg tggccctcga acggagcacg cgaccgcccc tgaggtatcg cctcctcgcg
     2821 aggtgtgtcg tcctcccggc acgtctgatg acacggatga gccccggccg tatccggagc
     2881 gtcctcgcct tcctggcggg ctccgccggt cccgccggct tcgcggaggc cctggccgcg
     2941 cgcaacgcgg tggtccacgt cagccgcagg tgcgcgggcc ccggttgcct acagcgctcc
     3001 atcgcgacct tcctcctctg ccggatcagg gggaccgtgc cggaatggtg cacgggtgtc
     3061 cgtgtgtccc cgttccgggc gcacgcctgg gtggaggtcg acggctcccc cgtgggagag
     3121 ctcggggaag tacggcactt ccacaaggtc atgacggtga ggccggtgat cgtggtccgt
     3181 gatgatgact gagatcgtcg cgctcgcggc gcagtacgcg ctgttcctga tcttcctggc
     3241 cgcctgggtc gggaagatca agggccctgg gcgtttcagc ggcttcgcga cggtgatctc
     3301 ccggttgacc gggtggtccc ctcctacggc gcgggccgtg ggcgtcaccg tggtcgccgg
     3361 tgaggtcctc gcctgccttc tcctgatctc gcccctcacc gccgcggcgg gggccgtcgt
     3421 ggccctgacc ctgctcaccg ccttcaccgg ggtggcggtc aaggccctgt ggagcggtat
     3481 ccgctccgag tgcgcctgct tcggcggatc ggattcggcc atcggttacg ccatgatcgt
     3541 gaggaacctg ctcctgatcg ccctcgccct gccaccgctc gtcgtacggg cggacctcgc
     3601 ggacctctcg gccaccggcg tggtcgcggt ggcgctcgga gccctcgcga tcgccctcct
     3661 cctcatcggg ttctacgatc cgctcgcccg ccgcgccacg gcggcgatca tcgcgacccg
     3721 tcgggcgacg cgaccccggg ccgggacggc ggggggtggc gacgatgtcg ggtgactcca
     3781 tcgaggtctc gaaggtcggg aaggtgttcg gaagcgtccg ggccctgaac gacgtctccc
     3841 tgtccgtctc ccccggaggg gtgctcggtc tgctgggaca caacggggcc ggcaagagca
     3901 cactcatcaa catcctctcc ggggtcctcg aaccgaccac gggaacggcg acggtcgcgg
     3961 ggttcgacgt ggtctcgcag gcgagccggg tccgacgtcg tatcggtgtg gccggacagt
     4021 acgtggccct ggacgaacag atctccgggc gggacaacct cgtcctgatc gcacggctgt
     4081 tgggggcgtc ccgtaaagag gcacgagagc ggacgcacga gcttctggag cggttcggca
     4141 tgaccgaatc cgccgatcgc gcggtgcgca cctactccgg cggcatgcga agaaggatcg
     4201 atctcgccgc gagcatcgtc ggcggccccg aggtcctgtt cctggacgag cccacggcgg
     4261 ggctggaccc cgagagccgc gcggagctgt gggcgatcgt ccaccgactg acggaggagg
     4321 gcgtcaccgt cctcttgacc acgcagtacc tggaggaggc cgaccgtctc tccgaccagg
     4381 tggtcctcct ctcccgggga cacgtcgtcg cctccggctc gccctcggag ctgaagaggg
     4441 gcgtgggccg gaggaaggcg tccctggggt tcaacgagag agagcgtttc tcggccgccc
     4501 gggacgaggt ccggaggatg ggactggtcg ccgacatcgg tgaggcgaac ttcgacatcg
     4561 acatcacgat cgcctcgcct cgcgacctgg gggccgtgat ccacgccctg gaggagagga
     4621 atctggggat cgaccgtctc cggctcttcg aacccagcct cgaagacgtc tacctcgccc
     4681 atgtctccac gcgatgaacg cggccacgaa gcgatcggtg aaggacccga tgacgatcga
     4741 cgccagagcc cgaaggtcca ccgtcccttg gaagtccgtg tcctggcggg gctgttccgt
     4801 ccccctacag atccacatcc tcacgaccag ggcgctccgc gcactctgga tggatccgaa
     4861 gctcgtcctc gtcagtgtcg cctcgcccct ggtcatgctc ctggtcctga gccaggtctt
     4921 cacgagcatc agcgagaccc ggagcttccc cggaggagtc gactacatcc agttcctgct
     4981 gcccgcgatc atgatcatgg gcgtcctaca gtccggcatg gggtccgggg cggccttggg
     5041 ccaggagatc aagaacggtt tcgtccatcg catgcgcacc ctgccggtgt ggccgggatc
     5101 cgtgcttctg ggaagaagcg cctcggacct ggtgaggagc ctgatacagc tctccgcgct
     5161 gtcgaccgcc gcgcacctgt tcttctcctt ccacgccccc ggtggagcgg tcggcatggc
     5221 ggtcgcgatg ctcatcgccc tgatgaccgg gaactgcctc ggctgggtgt tcatcgccat
     5281 ggcctgctgg ttccgcaacg ccgaggtcat gcagaccttc tccggcctgg ccatgttcgc
     5341 gctcatgttc ggcaccaacg cgttcgtccc tttggacggc ctgccggact ggctgcggag
     5401 tctctcccac gtcaacccca tgacctacgc catcacggcg gcccgaggtt ggatcctggg
     5461 cgatcccgac atgagcgcca ccctctacgc ggtgatcgga agcgcggtga tcgcgctgcc
     5521 ctgcgccctc ttggccgtca aggggttcgc acgccccctc ggaacgaact ga
//
