LOCUS       BGC0001725              6801 bp    DNA     linear   BCT 24-JUL-2016
DEFINITION  Bacillus megaterium plasmid pOXTB2 oxsA, oxsB, oxrB, oxrA genes,
            complete cds.
ACCESSION   BGC0001725
VERSION     BGC0001725
KEYWORDS    .
SOURCE      Priestia megaterium
  ORGANISM  Priestia megaterium
            Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Priestia.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-08 03:53:26
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..6801
                     /db_xref="taxon:1404"
                     /mol_type="genomic DNA"
                     /note="Bgl2 sites of pOXT1 was for cloning"
                     /organism="Priestia megaterium"
                     /plasmid="pOXTB2"
                     /strain="NK84-0128"
     subregion       1..6801
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0001725"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..6801
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            1722..2306
                     /gene="oxsA"
     CDS             1722..2306
                     /codon_start=1
                     /gene="oxsA"
                     /note="oxetanocin A biosynthetic enzyme"
                     /protein_id="BAA21630.1"
                     /transl_table=11
                     /translation="MSSLLDIIYQLRQVPRWDGSFQFEKEDVSQHSFSVIAISHILCEL
                     KETLEGKKINKEKLLLYALYHDVTEVVSTHIISPVKKNSILKDPFNAFREQIKNSLFDN
                     LPITLSDTLSTILNNNDLEIQEIVEHADHVDAYCKSCIEVHRGNKDFISIQRSLGDKLD
                     NLTKEYPYLKEFQNLFLKDFPLENKNYRYLN"
     gene            2318..4552
                     /gene="oxsB"
     CDS             2318..4552
                     /codon_start=1
                     /gene="oxsB"
                     /gene_functions="other (smcogs) SMCOG1277:Radical SAM
                     domain protein (Score: 154.3; E-value: 7.3e-47)"
                     /gene_kind="other"
                     /note="oxetanocin A biosynthetic enzyme"
                     /protein_id="BAA21631.1"
                     /transl_table=11
                     /translation="MQTYLSTKSIEYYLKELKEIFSQIWLKPSEIEKRCEELFKRSKEF
                     DYKRILVSGETDNTTLYVIEDSSKIHVFSPNRDLRENPLLMRWHPSWYEIESKEIYYKC
                     FLSCEELYEHLELPTVTLVNLCVIENFPIPRLNLSTGTLSSYLRKEQLAKVELIDMQVG
                     TTINQIIKNLLDSQPDIIGLSVNFGQKKLAFEILDLIYSHIENGDLSSIITVGNVIPSF
                     SPEQFFERYPSLLICDKEGEYTLRDLIKMLKKELKLDEVNGISYVDESGEVKHNVAETV
                     NFKEEVPTPSLDILGEISKFRGALTLETSRGCDYSRCTFCPRDHKLRSWRPLSVEQTLK
                     QLDDILRAGKHFNIKPHIYMADEEFIGELPNGTEAQRIIDICEGLLKREEKIKFDFAAR
                     ADSVYEPKRTKEWNVERLKMWHYCALAGADRIFIGVESGSNQQLKRYGKGTTSEQNIIA
                     LRLVSALGINLRIGFIMFDQLMKGLDNLKENLDFLERTDALMKPIDIGDMTYEELYDKL
                     LNDKEFIEKHKTGKPVYTIVSYMLASMEILMNTPYSRMVQLTERKEEVNLIMNDGKPDM
                     NMGRYATSFVDKTNGNLSEACQMWIDSNFGVMYTIKSLHKVANPREKKKLYSYMETHRE
                     ISHFLLKYLVYNLSPDKESQIILSDFLRMHSMEHILDNSKINVGDGSKENILNVMTNWQ
                     LIMEKLLRDVEADLNKGIITDSEDHRLHNTLKRWFSDMGNWSLINAYELN"
     gene            complement(4567..5544)
                     /gene="oxrB"
     CDS             complement(4567..5544)
                     /codon_start=1
                     /gene="oxrB"
                     /note="protein responsible for oxetanocin A resistance"
                     /protein_id="BAA21632.1"
                     /transl_table=11
                     /translation="MKIFDPIYGEYDLEPVLTELIHTKPVQRLKKIHQAGGCYLINGKW
                     DVTRYDHSVGVMLLIRYLNGSILEQIAGLLHDISHTAFSHVVDYVLDNDEEDYHEKIVD
                     KIVFSSEIPSILKKYDIDINLILDHSCWGILEQDLPLPCADRIDYTLRDMYYYQGENLE
                     DIQKFLNNLHVINNKIVIKNIESAEWFTKLYYKEVIDFFLHPLNVFSNRHLTKILTLAL
                     EKEIIHLDDFLTDDFTVMEKLTSSRDKQIINLISKFDLNHNHIIESKEDYEYSSKIKMR
                     EIDPLVNNQKKVECASSISPQIKQFTIEAYHKCEKGTYVKAVGI"
     gene            complement(5826..6383)
                     /gene="oxrA"
     CDS             complement(5826..6383)
                     /codon_start=1
                     /gene="oxrA"
                     /note="protein responsible for oxetanocin A resistance"
                     /protein_id="BAA21633.1"
                     /transl_table=11
                     /translation="MFNVFPIVQQLHEILWYLSEALEFQATNSIHDDLRISFEETERLI
                     KQTPENILKIDVHGHRMKVRELLLRTSELVRPKTSSKKLGKKFKRSSDYIGAKLRRANL
                     RGANLKGALFIAADLREADLRLTDLIGADFRDADLSGADLTGSIFLTQAQVNAAKGDLH
                     TKLPSALATPGHWRKRAQGLLN"
ORIGIN
        1 agatcttttt gctaggggtt taccactaag cccacctatt tggtgtttat gaatagatgt
       61 taatccctct cttaaaatag ttgtattact aactataagt ccgtctatgc caaccaataa
      121 tgcaatatca acagcttgtt caagctgatc taatgttaaa tcaggagcta cctttaaaat
      181 aataggtcga aacttccctg tgtttttaga taagttgttt cttgtattta ggattttttc
      241 taaaaaaggt tttagattgt ttgtatgttg taaatcacgt aggtttttag tattgggaga
      301 acttatgtta ataacaaagt aatctccata aagatataat aagtctaagc ttttcgtata
      361 atctgaagct gcatctacat tcgaagtatc tttatttttt cctagattaa tgccaatagg
      421 tgtctgaggt tttggtaatt tttcaaaatt ttctttaact ttatgtattc catgactatt
      481 aaatcccata cgattaatta atccattatc ttctggtaag cgataaagtc taggtaacgt
      541 atttccaggt tgtggagctg gtgtaactga cccaatctct acagatccaa aacctagggc
      601 agataggcct ggatatactt cagcatgttt gtcaaaccca gctgctaaac caacgggatt
      661 taaaaaatcc aatccccagt acgaggattt aagtttaggg tcgttagttg tcatcttttt
      721 attatactgg tctcttacaa aagaatttga ttgcattatt ttcagagccc taatacttaa
      781 attatgtgct ctttctggat ctacattaaa tagcattgga cggacagtag aataaaccat
      841 tagataatct cactttctat gtaatcttta ttttctggat aagatttcag attgaattac
      901 attaaaataa aatgtcttaa tcaatctata agtaattaga aaatcacttt ctaattataa
      961 gattataaca aaagggtatt ctataaaatc cctaaattgt aaaattataa aaatgagact
     1021 aaggaaataa tactaaattg tatccaaatc ttttttatgt aatttatcta gatatataag
     1081 actaatttaa gagagtttat ttttcactta gaaaatcagc gctaattttt ataagaatga
     1141 gtttttaaaa aaataggatt tttttatatt tgtttaaaag aacatttagg aaggtgcaat
     1201 ttattaggtg ctagaagccc ttgataagag agttttttag taagctattg tcaataactt
     1261 gccttatgga aactaaacat aaatatcttc tatattttag tgagttaaga gccatcacag
     1321 tcagctaact gattatttgt tgctctatag aagtgatagt aaaccttctt ggtcatatgc
     1381 ttttctttca tatttaatgc atattgaaaa attattggat ccattcagta ggtttttcta
     1441 tgtatggagt tttagtaact ctaaaaaatt taacaagtag ctgtttactt ttttaagtct
     1501 gttaatttta tgtgctattt atttaaatgt taatctctat aaaaaagtta catacctgaa
     1561 aacatagact aatttctgca agtatttgta acagatccat ttattatatt aaagagctcc
     1621 aattatcatt ttatggatat aaaagggtta ttttaccttg taataataaa ccatatatgt
     1681 taattatata attggaatat tttttcagga gaggtgtagc aatgagtagt ctattagaca
     1741 ttatttatca gctaagacag gttccgcggt gggatggctc ttttcaattt gaaaaagaag
     1801 atgtatctca gcatagtttc tcagtaatag caatatctca tatactttgt gagttaaaag
     1861 aaacccttga aggtaaaaaa atcaacaaag aaaaactctt gttatatgca ctttatcatg
     1921 atgttacaga agtcgtttct actcatatta ttagtcctgt gaagaagaat agtatattaa
     1981 aagatccttt taatgctttt agggagcaga ttaaaaatag tttgttcgat aatttgccaa
     2041 taacattgtc agacacatta tcaaccatct taaataataa cgatttagag atacaagaaa
     2101 ttgtggagca tgcagaccat gttgatgcgt actgcaaatc atgtatagaa gtacatagag
     2161 gaaataaaga ttttatctct attcagagaa gccttggaga taaactagat aacttgacta
     2221 aagaatatcc atacctaaaa gaatttcaaa atctatttct taaggatttt cccttagaaa
     2281 ataaaaatta caggtacctt aactgaggag gataaaaatg cagacttatt tatcaactaa
     2341 aagtattgag tactatttaa aggagcttaa ggagatattt tcacaaatat ggttgaaacc
     2401 ttctgagata gaaaaaaggt gtgaagagct ttttaaacgt agtaaagaat ttgattataa
     2461 aaggatactt gtatcagggg aaactgataa cacaaccctc tacgtaatag aagatagttc
     2521 caagatacat gttttttcac ctaatagaga tttaagagaa aaccctctat taatgcgttg
     2581 gcacccttcc tggtatgaga ttgagagcaa agaaatttac tataaatgct tcctgagttg
     2641 tgaagaatta tatgaacatt tagaacttcc tactgtaaca ttagtaaatt tatgcgtaat
     2701 agaaaatttc cccattccaa gattaaactt aagtacggga actttatctt cttacttaag
     2761 aaaagaacaa ttagctaaag ttgagttaat tgatatgcaa gtagggacta caattaacca
     2821 aattataaaa aacctcttgg atagtcagcc tgatattatt ggcttatctg tgaattttgg
     2881 gcaaaagaag ttagccttcg agattttaga tttgatttat tcacatatag agaatggaga
     2941 cttatcttca atcattactg taggaaacgt aattccttca ttctctccag aacaattctt
     3001 tgaaaggtac ccttcattgc tgatttgtga taaggagggt gaatatacac taagagacct
     3061 gataaaaatg ctaaaaaaag aacttaaact tgatgaagta aatggaatat catatgtaga
     3121 tgagtctgga gaagtaaagc ataacgtagc tgaaactgtt aactttaagg aagaagtccc
     3181 cacaccttca ctggatattt taggcgaaat atcgaagttt aggggagcat tgacactaga
     3241 aactagtagg ggttgtgatt attctcgatg taccttctgc ccaagagatc ataaattaag
     3301 atcatggcga ccattatctg tcgaacaaac tcttaaacaa ttagatgata ttttaagagc
     3361 cggtaaacat ttcaatataa agccccatat ttatatggct gacgaagaat ttattggtga
     3421 gctaccaaat ggaacagagg ctcagagaat tattgatata tgtgaggggt tattaaaaag
     3481 agaggaaaaa ataaaatttg attttgctgc tagagcagac tctgtgtatg aaccaaaaag
     3541 aacaaaagag tggaacgttg aaaggctaaa aatgtggcat tactgcgctc tggcaggtgc
     3601 tgatagaatc tttattggag tagaatcagg atccaatcaa caattgaaga gatatgggaa
     3661 gggcaccaca tcagaacaaa atataattgc tttgagatta gtaagtgcat tgggtatcaa
     3721 tttgagaatt ggttttatca tgtttgatca attaatgaag ggattagata acctcaaaga
     3781 aaacctagat tttctagaaa gaacagatgc tctcatgaaa cctattgata ttggtgatat
     3841 gacatatgaa gaattatatg acaaactttt aaacgacaaa gaatttattg aaaaacataa
     3901 aacaggtaaa cctgtctata ctattgtttc atatatgtta gcttctatgg aaattctgat
     3961 gaatacccct tattcaagaa tggttcaatt aacagaacga aaagaagaag tgaaccttat
     4021 aatgaatgat ggaaagccgg atatgaacat gggaagatat gcaacttcct ttgtagataa
     4081 aacaaacggt aatttaagtg aggcttgtca aatgtggata gatagtaact ttggggtaat
     4141 gtacacaatt aagagtttgc ataaagttgc taatcctaga gaaaagaaaa agttgtatag
     4201 ttatatggaa acacatagag agattagtca ttttctttta aagtatcttg tatataatct
     4261 tagtcccgat aaggaaagtc aaattatctt atcagatttt ctaagaatgc attcaatgga
     4321 acacatttta gataattcta aaataaatgt gggggatggt tctaaagaaa atattttgaa
     4381 tgtaatgaca aattggcaac taattatgga aaaactttta agagatgtag aggctgattt
     4441 aaataaaggg attattactg atagcgaaga tcatagactc cataatacgt taaaacgatg
     4501 gtttagtgat atgggtaact ggtctttaat aaatgcttac gagctaaact aaaggcagtg
     4561 ggaattctat attcccactg cttttacata ggtacccttt tcacatttat gataagcctc
     4621 tatagtaaat tgtttaatct gtggtgaaat ggaagaggcg cactcaactt tcttctgatt
     4681 attaactaat ggatctattt ccctcatttt tattttagag ctatattcat agtcttcttt
     4741 tgattctata atatgattat gattaagatc aaacttacta attaagttta ttatttgttt
     4801 atctctacta gaagttaatt tttccattac agtaaaatcg tctgtaagga agtcatctaa
     4861 atgaataatt tctttttcaa gagccaaggt tagaatttta gttaaatgtc tattcgagaa
     4921 gacatttaat ggatgtaaaa agaaatctat tacttcttta taatataatt tagtaaacca
     4981 ttctgccgac tcaatattct ttatcactat cttattattg ataacatgaa ggttatttaa
     5041 aaatttttga atatcctcta aattttcgcc ttgataataa tacatatccc tcaacgtata
     5101 atcaattctg tctgcacaag gtaaaggcaa atcttgttct aaaattcccc aacaactgtg
     5161 gtcgagaatt aaattaatat caatatcata tttttttaat atacttggaa tttctgatga
     5221 aaaaacaatt ttatctacaa tcttttcatg ataatcttcc tcatcattat ctaatacata
     5281 gtctactacg tgggaaaagg ccgtatgtga gatgtcatga agtaaaccag caatctgttc
     5341 taatatactt ccatttaaat atcttattaa caacattact cctacggaat gatcgtaacg
     5401 agtaacatcc cacttgccat taattaaata acatccccct gcctggtgta ttttttttaa
     5461 acgttgaaca ggctttgtat gtattagttc tgttaaaact ggttccaaat catattctcc
     5521 ataaattgga tcaaatattt tcattatgtt ccccctttaa aataaaattt taaaataatc
     5581 ttctttctat ttaattatgg aataattagg gattttgtac aactatttta aaatatattt
     5641 taaatattag ctactccatt accgatattt acctaaaaat aagttttttt acattatata
     5701 tagatatttc cttctgacta tatatagcta ctatacatac attattaacg ctggggagta
     5761 atatacattg ttatttttta tacttaacgt aatcaaaatt ataggaacga aaagaagctc
     5821 aagaactagt taagtagtcc ttgagctctt tttctccagt gtcctggagt cgctaaagca
     5881 gagggcaact ttgtatgtaa atcgcctttt gctgcattca cctgagcttg ggtaaggaaa
     5941 atacttcctg taagatcagc accacttaag tcagcatctc tgaagtctgc tccaataaga
     6001 tccgttaatc tgaggtcagc ttctctaaga tcagcagcta taaataatgc tccctttaaa
     6061 ttagctcctc taagattagc tcttcttaac ttagccccaa tatagtcact actacgctta
     6121 aatttctttc ctaacttttt gctagatgtt ttgggacgaa ccaattcact tgtacgtaaa
     6181 agtaattctc tgactttcat tcgatgccca tgcacatcaa tttttaaaat gttttctggt
     6241 gtttgtttaa ttaaacgttc cgtttcttca aaggaaatcc ttaaatcatc atgaatggaa
     6301 ttcgtagctt gaaattcaag tgcctcgctt aaataccaaa gtatttcatg caattgctga
     6361 acaattggaa acacattaaa catttcttta gctcttgctt tattacctct ccaatcattt
     6421 ccttcataaa tcgtttgaga aaccctttgg ccagcaccga aacattcata tcccacacag
     6481 cctttaaatc ccgtagtgat aagattcttg tgaatggaac atgaatagtt tgactgtaaa
     6541 tgacggcaag gagttccacc atctttgtca aaggcaaaat cagcagattt tgcgtaaggt
     6601 aaagctacgc agcacaaacc aaaacaattt gagcagtccg aacgtaaatt ttctcgaact
     6661 ttattatcta tctctagttt gttaggcaag aaaacacatt cctttcttat aatttcctgt
     6721 aattttataa taattggaat tataagaaaa ataaaggttg ttactgattt agatttatta
     6781 ctgtataaga tatacagatc t
//
