BGC0000115: nystatin A1 biosynthetic gene cluster from Streptomyces noursei ATCC 11455
Shows the layout of the region, marking coding sequences and areas of interest. Clicking a gene will select it and show any relevant details. Clicking an area feature (e.g. a candidate cluster) will select all coding sequences within that area. Double clicking an area feature will zoom to that area. Multiple genes and area features can be selected by clicking them while holding the Ctrl key.
View antiSMASH-generated output
Download Cluster GenBank file
Download region SVG
Location: 1 - 123,580 nt. (total: 123,580 nt).
This entry is originally from NCBI GenBank AF263912.1.

Legend:

core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
reset zoomreset view
zoomzoom to selection
Gene details
Shows details of the most recently selected gene, including names, products, location, and other annotations.
Select a gene to view the details available for it
General
Compounds
Genes
Polyketide
Saccharide
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0000115
Short description nystatin A1 biosynthetic gene cluster from Streptomyces noursei ATCC 11455
Status Minimal annotation: no
A minimal annotation only contains information on the BGC loci and one or more linked chemical product(s)

Completeness: complete
Whether the loci encodes everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • Polyketide (Polyene)
  • Saccharide (hybrid/tailoring)
Loci NCBI GenBank: AF263912.1
Compounds
  • nystatin A1
Species Streptomyces noursei ATCC 11455 [taxonomy]
References
Chemical products information
nystatin A1
Copy SMILES
C47H75N1O17
Chemical database entries
PubCHEM
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • AAF71762.1
  • nysF
 46 - 783 (-) nysF
  • Activation / processing
Knock-out
copy AA seq
copy Nt seq
  • AAF71763.1
  • nysG
 867 - 2684 (-) nysG
  • Transport
Knock-out
copy AA seq
copy Nt seq
  • AAF71764.1
  • nysH
 2662 - 4416 (-) nysH
  • Transport
Knock-out
copy AA seq
copy Nt seq
  • AAF71765.1
  • nysDIII
 4714 - 5748 (+) nysDIII
  • Precursor biosynthesis
Activity assay
copy AA seq
copy Nt seq
  • AAF71766.1
  • nysI
 5930 - 34363 (+) nysI
  • Scaffold biosynthesis
Sequence-based prediction
copy AA seq
copy Nt seq
  • AAF71767.1
  • nysJ
 34384 - 50691 (+) nysJ
  • Scaffold biosynthesis
Sequence-based prediction
copy AA seq
copy Nt seq
  • AAF71768.1
  • nysK
 50747 - 56947 (+) nysK
  • Scaffold biosynthesis
Sequence-based prediction
copy AA seq
copy Nt seq
  • AAF71769.1
  • nysL
 57095 - 58279 (+) nysL
  • Tailoring (Monooxygenation)
Activity assay
copy AA seq
copy Nt seq
  • AAF71770.1
  • nysM
 58378 - 58572 (-) nysM
  • Activation / processing
Sequence-based prediction
copy AA seq
copy Nt seq
  • AAF71771.1
  • nysN
 58637 - 59833 (-) nysN
  • Tailoring (Monooxygenation)
Knock-out
copy AA seq
copy Nt seq
  • AAF71772.1
  • nysDII
 59830 - 60888 (-) nysDII
  • Precursor biosynthesis
Sequence-based prediction
copy AA seq
copy Nt seq
  • AAF71773.1
  • nysDI
 60909 - 62429 (-) nysDI
  • Tailoring (Glycosylation)
Knock-out
copy AA seq
copy Nt seq
  • AAF71774.1
  • nysA
 62659 - 66759 (+) nysA
  • Scaffold biosynthesis
Knock-out
copy AA seq
copy Nt seq
  • AAF71775.1
  • nysB
 66805 - 76383 (+) nysB
  • Scaffold biosynthesis
Sequence-based prediction
copy AA seq
copy Nt seq
  • AAF71776.1
  • nysC
 76403 - 109693 (+) nysC
  • Scaffold biosynthesis
Knock-out
copy AA seq
copy Nt seq
  • AAF71777.1
  • nysE
 110113 - 110868 (+) nysE
  • Unknown
Knock-out
copy AA seq
copy Nt seq
  • AAF71778.1
  • nysRI
 111258 - 114158 (+) nysRI
  • Regulation
Knock-out
copy AA seq
copy Nt seq
  • AAF71779.1
  • nysRII
 114182 - 117043 (+) nysRII
  • Regulation
Knock-out
copy AA seq
copy Nt seq
  • AAF71780.1
  • nysRIII
 117033 - 119816 (+) nysRIII
  • Regulation
Knock-out
copy AA seq
copy Nt seq
  • AAF71781.1
 120268 - 120900 (+) ORF4
  • Regulation
Knock-out
copy AA seq
copy Nt seq
  • AAF71782.1
 121589 - 122350 (+) ORF3
  • Unknown
Knock-out
copy AA seq
copy Nt seq
  • AAF71783.1
 122404 - 123468 (-) ORF2
copy AA seq
copy Nt seq
Polyketide-specific information
Subclass Polyene
Starter unit Malonyl-CoA
Cyclic? yes
Release type
  • Macrolactonization
Polyketide-synthases
Genes Properties Modules
nysK
+
nysK
+
nysC
+
nysJ
+
nysB
+
nysE
+
nysI
+
nysA 		Synthase subclass: Modular type I
Thioesterases:
  • nysK (Unknown)
  • nysE (Unknown)
Module 1
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: nysA
Core domains: Ketosynthase, Acyltransferase, Dehydratase, Thiolation (ACP/PCP)
Module 2
Specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
Genes: nysB
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: D-OH
Module 3
Specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
Genes: nysB
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: D-OH
Module 4
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: nysC
Core domains: Ketosynthase, Acyltransferase, Dehydratase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 5
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: nysC
Core domains: Ketosynthase, Acyltransferase, Dehydratase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 6
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: nysC
Core domains: Ketosynthase, Acyltransferase, Dehydratase, Enoylreductase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 7
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: nysC
Core domains: Ketosynthase, Acyltransferase, Dehydratase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 8
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: nysC
Core domains: Ketosynthase, Acyltransferase, Dehydratase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 9
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: nysC
Core domains: Ketosynthase, Acyltransferase, Dehydratase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 10
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: nysI
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: D-OH
Module 11
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: nysI
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: D-OH
Module 12
Specificity: Methylmalonyl-CoA
Evidence for specificity: Structure-based inference
Genes: nysI
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 13
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: nysI
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 14
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: nysI
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 15
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: nysI
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 16
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: nysJ
Core domains: Ketosynthase, Acyltransferase, Dehydratase, Enoylreductase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 17
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: nysJ
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 18
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: nysJ
Core domains: Ketosynthase, Acyltransferase, Dehydratase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module 19
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: nysK
Core domains: Ketosynthase, Acyltransferase, Dehydratase, Ketoreductase, Thiolation (ACP/PCP), Thioesterase
KR-domain stereochemistry: Unknown
Saccharide-specific information
Subclass hybrid/tailoring
Glycosyltransferases
nysDI
Specificity: mycoamine
Evidence:
  • Sequence-based prediction
Annotation changelog
MIBiG version Submitter Notes
1.0
  • Hidden contributor (ID: MU4LZLKCEF7Q67JF6ZEMKX3R, no GDPR consent given).
  • Submitted
2.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Migrated from v1.4
3.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Removed gene names of 'No gene ID'
  • Removed ketoreductase stereochemistry annotation from modules without ketoreductases
  • Sorted modules by module number
3.1
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Update chemical activity to schema version 2.11
Detailed domain annotation
Shows NRPS- and PKS-related domains for each feature that contains them. Click on each domain for more information about the domain's location, consensus monomer prediction, and other details.
A glossary is available here.
Selected features only
Show module domains
Similar known gene clusters
Shows clusters from the MiBIG database that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
Click on reference genes to show details of similarities to genes within the current region.
Click on an accession to open that entry in the MiBIG database.