BGC0000187: asukamycin biosynthetic gene cluster from Streptomyces nodosus subsp. asukaensis
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Location: 1 - 63,922 nt. (total: 63,922 nt).
This entry is originally from NCBI GenBank GQ926890.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
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Gene details
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General
Compounds
Genes
Biosynthesis
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0000187
Short description asukamycin biosynthetic gene cluster from Streptomyces nodosus subsp. asukaensis
Status Quality: questionable
The quality level of this entry.

Status: active
The status of this entry.

Completeness: complete
Whether the entry covers everything needed for the pathway producing the compound(s)
Remarks "3-amino-4-hydroxybenzoic acid is the precursor for 2-amino-4-hydroxy-5,6-epoxycyclohex-2-enone"
Biosynthetic class(es)
  • PKS (Type II)
Loci
GQ926890.1
via Knock-out studies, Enzymatic assays, Heterologous expression, Gene expression correlated with compound production
Compounds
  • asukamycin
Species Streptomyces nodosus subsp. asukaensis [taxonomy]
References
Chemical products information
asukamycin Evidence:
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C31H34N2O7
Chemical database entries
NPAtlas
PubCHEM
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • ADI58659.1
 29 - 1096 (+) hypothetical protein
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    • ADI58660.1
    		 1341 - 1643 (+) hypothetical protein
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      • ADI58661.1
      		 1866 - 3689 (+) hypothetical protein
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        • ADI58662.1
        		 3689 - 4354 (+) hypothetical protein
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          • ADI58663.1
          		 4347 - 5102 (+) hypothetical protein
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            • ADI58664.1
            		 5099 - 5866 (+) hypothetical protein
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              • ADI58665.1
              		 5853 - 7118 (-) hypothetical protein
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                • ADI58666.1
                		 7370 - 7933 (+) hypothetical protein
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                  • ADI58667.1
                  		 8052 - 8630 (+) hypothetical protein
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                    • ADI58668.1
                    		 8737 - 9168 (+) hypothetical protein
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                      • ADI58669.1
                      		 9251 - 10393 (-) hypothetical protein
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                        • ADI58670.1
                        		 10390 - 10692 (-) hypothetical protein
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                          • ADI58671.1
                          		 10698 - 11426 (-) hypothetical protein
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                            • ADI58672.1
                            		 11463 - 13016 (-) hypothetical protein
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                              • ADI58673.1
                              		 13013 - 14686 (-) hypothetical protein
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                                • ADI58674.1
                                		 14761 - 16332 (-) hypothetical protein
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                                  • ADI58675.1
                                  		 16427 - 17881 (-) hypothetical protein
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                                    • ADI58676.1
                                    		 18185 - 19852 (-) hypothetical protein
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                                      • ADI58677.1
                                      		 19965 - 20771 (-) hypothetical protein
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                                        • ADI58678.1
                                        		 20849 - 21142 (+) hypothetical protein
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                                          • ADI58679.1
                                          		 21139 - 22389 (+) hypothetical protein
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                                            • ADI58680.1
                                            		 22419 - 23357 (+) hypothetical protein
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                                              • ADI58681.1
                                              		 23354 - 23872 (+) hypothetical protein
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                                                • ADI58682.1
                                                		 23921 - 24289 (-) hypothetical protein
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                                                  • ADI58683.1
                                                  		 24430 - 24885 (-) hypothetical protein
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                                                    • ADI58684.1
                                                    		 25156 - 25386 (+) hypothetical protein
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                                                      • ADI58623.1
                                                      • asuR4
                                                      		 25411 - 25836 (-) Transcriptional regulation
                                                      • Regulation
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                                                      • ADI58624.1
                                                      • asuR3
                                                      		 25976 - 26899 (-) Transcriptional regulation
                                                      • Regulation
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                                                      • ADI58625.1
                                                      • asuR2
                                                      		 26960 - 27535 (-) Transcriptional regulation
                                                      • Regulation
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                                                      • ADI58626.1
                                                      • asuR1
                                                      		 27554 - 28204 (-) Transcriptional regulation
                                                      • Regulation
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                                                      • ADI58627.1
                                                      • asuC1
                                                      		 28309 - 28968 (-) Phosphopantetheinyl transferase
                                                      • Scaffold biosynthesis
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                                                      • ADI58628.1
                                                      • asuE1
                                                      		 29329 - 30552 (+) Protoasukamycin 4-hydroxylase
                                                      • Tailoring
                                                      • Knock-out
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                                                      • ADI58629.1
                                                      • asuB4
                                                      		 30611 - 32614 (-) 2,4-DienoylCoA reductase
                                                      • Precursor biosynthesis
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                                                      • ADI58630.1
                                                      • asuB3
                                                      		 32658 - 33491 (-) 1-Cyclohexenylcarbonyl CoA reductase
                                                      • Precursor biosynthesis
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                                                      • ADI58631.1
                                                      • AsuB2
                                                      		 33488 - 34672 (-) Acyl-CoA dehydrogenase
                                                      • Precursor biosynthesis
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                                                      • ADI58632.1
                                                      • asuB1
                                                      		 34669 - 37620 (-) 5-Enolpyruvylshikimate-3-phosphate synthase/CHC-CoA ligase
                                                      • Precursor biosynthesis
                                                      • Knock-out
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                                                      • ADI58633.1
                                                      • asuC5
                                                      		 37663 - 37917 (-) Asukamycin KSIII associated ACP
                                                      • Scaffold biosynthesis
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                                                      • ADI58634.1
                                                      • asuC4
                                                      		 37928 - 39013 (-) Asukamycin ketosynthase III
                                                      • Scaffold biosynthesis
                                                      • Knock-out
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                                                      • ADI58635.1
                                                      • asuC3
                                                      		 39010 - 40026 (-) Asukamycin ketosynthase III
                                                      • Scaffold biosynthesis
                                                      • Knock-out
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                                                      • ADI58636.1
                                                      • AsuC2
                                                      		 40108 - 40917 (-) Arylamine N-acyltransferase
                                                      • Scaffold biosynthesis
                                                      • Knock-out
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                                                      • ADI58637.1
                                                      • asuC6
                                                      		 41274 - 41729 (+) Thioesterase
                                                      • Scaffold biosynthesis
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                                                      • ADI58638.1
                                                      • asuE2
                                                      		 41746 - 42303 (+) Flavin reducatse
                                                      • Tailoring
                                                      • Activity assay
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                                                      • ADI58639.1
                                                      • asuC7
                                                      		 42365 - 43069 (+) Ketoreductase
                                                      • Scaffold biosynthesis
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                                                      • ADI58640.1
                                                      • asuC10
                                                      		 43147 - 43431 (-) Unknown
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                                                        • ADI58641.1
                                                        • asuC9
                                                        		 43428 - 43880 (-) Acyl dehydratase
                                                        • Scaffold biosynthesis
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                                                        • ADI58642.1
                                                        • asuC8
                                                        		 43877 - 44335 (-) Acyl dehydratase
                                                        • Scaffold biosynthesis
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                                                        • ADI58643.1
                                                        • asuA2
                                                        		 44332 - 45747 (-) 3,4-AHBA carboxyl group adenylation
                                                        • Activation / processing
                                                        • Activity assay
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                                                        • ADI58644.1
                                                        • asuA1
                                                        		 45867 - 46985 (-) 3,4-AHBA synthase
                                                        • Precursor biosynthesis
                                                        • Knock-out
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                                                        • ADI58645.1
                                                        • asuD1
                                                        		 47448 - 49043 (+) Amide synthase
                                                        • Precursor biosynthesis
                                                        • Knock-out
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                                                        • ADI58646.1
                                                        • asuD2
                                                        		 49040 - 50269 (+) 2-Oxoamine synthase
                                                        • Precursor biosynthesis
                                                        • Knock-out
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                                                        • ADI58647.1
                                                        • asuD3
                                                        		 50506 - 51912 (+) 5-Aminolevulinate CoA ligase
                                                        • Precursor biosynthesis
                                                        • Knock-out
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                                                        • ADI58648.1
                                                        • asuE3
                                                        		 51995 - 53110 (-) 4-Hydroxyprotoasukamycin epoxidase
                                                        • Tailoring
                                                        • Activity assay
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                                                        • ADI58649.1
                                                        • asuC14
                                                        		 53157 - 53879 (-) Ketosynthase I/II
                                                        • Scaffold biosynthesis
                                                        • Knock-out
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                                                        • ADI58650.1
                                                        • asuC13
                                                        		 53935 - 55116 (-) Ketosynthase I/II
                                                        • Scaffold biosynthesis
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                                                        • ADI58651.1
                                                        • asuC12
                                                        		 55118 - 55381 (-) 3,4-AHBA carrier protein
                                                        • Activation / processing
                                                        • Activity assay
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                                                        • ADI58652.1
                                                        • asuC11
                                                        		 55378 - 55653 (-) KSI/II associated ACP
                                                        • Scaffold biosynthesis
                                                        • Knock-out
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                                                        • ADI58653.1
                                                        • asuA3
                                                        		 55734 - 56570 (-) Condensation of L-aspartate-4-semialdehyde and dihydroxyacetone phosphate
                                                        • Precursor biosynthesis
                                                        • Knock-out
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                                                        • ADI58654.1
                                                        • asuC15
                                                        		 56829 - 57611 (+) Type II thioesterase
                                                        • Activation / processing
                                                        • Knock-out
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                                                        • ADI58655.1
                                                        • asuM1
                                                        		 57721 - 59259 (+) Asukamycin exporter
                                                        • Transport
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                                                        • ADI58657.1
                                                        • asuR5
                                                        		 59539 - 60303 (+) Transcriptional regulation
                                                        • Regulation
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                                                        • ADI58656.1
                                                        • asuH1
                                                        		 60425 - 61579 (+) Hypothetical
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                                                          • ADI58658.1
                                                          • asuR6
                                                          		 61590 - 62432 (-) Transcriptional regulation
                                                          • Regulation
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                                                          Biosynthesis information
                                                          Annotation changelog

                                                          Entry version: 4

                                                          Date
                                                          Changes
                                                          Submitters
                                                          Reviewers
                                                          2022-10-07
                                                          Update chemical activity to schema version 2.11
                                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                                                          Entry version: 3

                                                          Date
                                                          Changes
                                                          Submitters
                                                          Reviewers
                                                          2022-09-15
                                                          Updated bioactivity data
                                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                          2022-09-15
                                                          Added AMP-binding domain substrate specificities
                                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                                                          Entry version: 2

                                                          Date
                                                          Changes
                                                          Submitters
                                                          Reviewers
                                                          2019-10-16
                                                          Migrated from v1.4
                                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                          2019-10-16
                                                          Updated compound(s) information (NPAtlas curation)
                                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                                                          Entry version: 1

                                                          Date
                                                          Changes
                                                          Submitters
                                                          Reviewers
                                                          2015-06-12
                                                          Submitted
                                                          • (ID: VQJTWYI3STDVOHG36EUY4LM4)
                                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
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