BGC0000459: griseoviridin biosynthetic gene cluster from Streptomyces griseoviridis
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Location: 13,530 - 118,200 nt. (total: 104,671 nt).
This entry is originally from NCBI GenBank JX508597.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
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Gene details
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General
Compounds
Genes
Polyketide
NRP
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0000459
Short description griseoviridin biosynthetic gene cluster from Streptomyces griseoviridis
Status Minimal annotation: no
A minimal annotation only contains information on the BGC loci and one or more linked chemical product(s)

Completeness: complete
Whether the loci encodes everything needed for the pathway producing the compound(s)
Remarks "VG is synthesised by the NRPS within this cluster whereas GV is hybrid PK-NRP and as such utilises the PKS machinery also."
Biosynthetic class(es)
  • NRP (Cyclic depsipeptide)
  • Polyketide (Other)
Loci NCBI GenBank: JX508597.1
Compounds
  • griseoviridin
  • fijimycin A
Species Streptomyces griseoviridis [taxonomy]
References
Chemical products information
griseoviridin [synonyms: virginiamycin m, pristinamycin IIA]
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C22H27N3O7S1
Chemical database entries
NPAtlas
fijimycin A
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C44H62N8O11
Chemical database entries
NPAtlas
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • AGN74872.1
 13530 - 14696 (+) SARP-type regulator
  • Regulation
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  • AGN74873.1
 14816 - 16408 (-) major facilitator transporter
  • Transport
Sequence-based prediction
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  • AGN74874.1
 16726 - 17847 (-) branched-chain amino acid aminotransferase
  • Precursor biosynthesis
Sequence-based prediction
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  • AGN74875.1
 17916 - 18935 (-) methyltransferase
  • Precursor biosynthesis
Sequence-based prediction
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  • AGN74876.1
 19073 - 26989 (-) nonribosomal peptide synthetase
  • Scaffold biosynthesis
Sequence-based prediction
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  • AGN74877.1
 27311 - 28057 (+) type ii thioestrerase
  • Scaffold biosynthesis
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  • AGN74878.1
 28052 - 29134 (-) flavin-dependent oxidoreductase
  • Precursor biosynthesis
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  • AGN74879.1
 29368 - 29613 (-) hypothetical protein
  • Unknown
Sequence-based prediction
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  • AGN74880.1
 29782 - 31404 (+) 3-hydroxypicolinic acid:AMP ligase
  • Scaffold biosynthesis
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  • AGN74881.1
 31408 - 32709 (+) L-lysine 2-aminotransferase
  • Precursor biosynthesis
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  • AGN74882.1
 32763 - 32951 (+) 4-oxalocrotonate tautomerase
  • Unknown
Sequence-based prediction
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  • AGN74883.1
 33036 - 34343 (-) phenylacetyl-CoA dioxygenase
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  • AGN74884.1
 34358 - 34609 (-) MbtH domain-containing protein
  • Scaffold biosynthesis
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  • AGN74885.1
 34683 - 51854 (-) nonribosomal peptide synthetase
  • Scaffold biosynthesis
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  • AGN74886.1
 51851 - 56554 (-) nonribosomal peptide synthetase
  • Scaffold biosynthesis
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  • AGN74887.1
 57108 - 58265 (+) branched-chain alpha-keto acid dehydrogenase E1 subunit alpha
  • Precursor biosynthesis
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  • AGN74888.1
 58262 - 59317 (+) branched-chain alpha-keto acid dehydrogenase E1 subnuit beta
  • Precursor biosynthesis
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  • AGN74889.1
 59323 - 60720 (+) branched-chain alpha-keto acid dehydrogenase E2
  • Precursor biosynthesis
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  • AGN74890.1
 60959 - 62614 (+) ABC transporter
  • Transport
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  • AGN74891.1
 62773 - 63963 (-) P450-like monooxygenase
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  • AGN74892.1
 64158 - 77825 (+) nonribosomal peptide synthetase/polyketide synthase hybrid protein
  • Scaffold biosynthesis
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  • AGN74893.1
 77828 - 84568 (+) nonribosomal peptide synthetase/polyketide synthase hybrid protein
  • Scaffold biosynthesis
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  • AGN74894.1
 84740 - 91246 (+) nonribosomal peptide synthetase/polyketide synthase hybrid protein
  • Scaffold biosynthesis
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  • AGN74895.1
 91257 - 99092 (+) nonribosomal peptide synthetase/polyketide synthase hybrid protein
  • Scaffold biosynthesis
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  • AGN74896.1
 99089 - 104953 (+) nonribosomal peptide synthetase/polyketide synthase hybrid protein
  • Scaffold biosynthesis
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  • AGN74897.1
 104950 - 105900 (+) acyltransferase
  • Scaffold biosynthesis
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  • AGN74898.1
 105894 - 108320 (+) nonribosomal peptide synthetase
  • Scaffold biosynthesis
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  • AGN74899.1
 108570 - 109964 (+) major facilitator transporter
  • Transport
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  • AGN74900.1
 109996 - 110889 (-) streptogramin B lyase
  • Resistance/immunity
Sequence-based prediction
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  • AGN74901.1
 111137 - 112285 (-) sarcosine oxidase
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  • AGN74902.1
 112395 - 113237 (-) SARP-type regulator
  • Regulation
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  • AGN74903.1
 113627 - 114274 (-) TetR-type regulator
  • Regulation
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  • AGN74904.1
 114756 - 115520 (+) ketoreductase
  • Regulation
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  • AGN74905.1
 115710 - 116678 (+) AfsA-like gamma-butyrolactone biosynthetic protein
  • Regulation
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  • AGN74906.1
 116703 - 117386 (-) gamma-butyrolactone receptor
  • Regulation
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  • AGN74907.1
 117511 - 118200 (-) phosphatase
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Polyketide-specific information
Subclass Other
Starter unit Acetyl-CoA
Cyclic? yes
Release type
  • Macrolactonization
Polyketide-synthases
Genes Properties Modules
AGN74877.1
+
AGN74892.1
+
AGN74893.1
+
AGN74895.1
+
AGN74894.1
+
AGN74897.1 		Synthase subclass: Trans-AT type I
Trans-acyltansferases: AGN74897.1
Thioesterases:
  • AGN74877.1 (Unknown)
Module B1
Genes: AGN74892.1
Core domains: Ketosynthase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: D-OH
Module B2
Genes: AGN74892.1
Core domains: Ketosynthase, Dehydratase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: D-OH
Module B4
Genes: AGN74893.1
Core domains: Ketosynthase, Dehydratase, Ketoreductase, Thiolation (ACP/PCP), Ketosynthase
KR-domain stereochemistry: D-OH
Module B5
Genes: AGN74894.1
Core domains: Ketosynthase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: L-OH
Module B6
Genes: AGN74894.1
Core domains: Ketosynthase, Thiolation (ACP/PCP)
Module B8
Genes: AGN74895.1
Core domains: Ketosynthase, Thiolation (ACP/PCP)
Module 0
Genes: AGN74892.1
Core domains: Thiolation (ACP/PCP)
NRP-specific information
Subclass Cyclic depsipeptide
Cyclic? yes
Release type
  • Macrolactonization
NRP-synthases
Gene Modules
AGN74880.1
Module 0
Specificity: 3-hydroxypicolinic acid
Evidence for specificity:
  • Sequence-based prediction
AGN74876.1
Module 1
Specificity: threonine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
Module 2
Specificity: leucine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
AGN74886.1
Module A3
Specificity: 4R-hydroxyproline
Evidence for specificity:
  • Structure-based inference
Condensation domain type: DCL
AGN74885.1
Module 4
Specificity: glycine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: DCL
Modification domains: Methylation
Module 5
Specificity: 3-methylleucine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
Modification domains: Methylation
Module 6
Specificity: alanine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
Module A7
Specificity: phenylglycine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
Modification domains: Methylation
AGN74892.1
Module B3
Specificity: glycine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: Unknown
AGN74895.1
Module B7
Specificity: serine
Evidence for specificity:
  • Structure-based inference
Modification domains: Unknown
AGN74898.1
Module B9
Specificity: cysteine
Evidence for specificity:
  • Structure-based inference
AGN74896.1
Module B10 [inactive]
Condensation domain type: Unknown
Module 9
Condensation domain type: Unknown
Annotation changelog
MIBiG version Submitter Notes
1.0
  • Hidden contributor (ID: AADADFAEMFE2AOMR24EUYNFH, no GDPR consent given).
  • Submitted
2.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Migrated from v1.4
  • Updated compound(s) information (NPAtlas curation)
3.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Corrected NRP module activity
  • Removed ketoreductase stereochemistry annotation from modules without ketoreductases
  • Sorted modules by module number
  • Corrected gene identifiers
  • Updated bioactivity data
  • Updated NRP substrate specificities
3.1
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Update chemical activity to schema version 2.11
Detailed domain annotation
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