BGC0000950: napsamycin A biosynthetic gene cluster from Streptomyces sp. DSM 5940
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Location: 1 - 35,298 nt. (total: 35,298 nt).
This entry is originally from NCBI GenBank HQ287563.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
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General
Compounds
Genes
Biosynthesis
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0000950
Short description napsamycin A biosynthetic gene cluster from Streptomyces sp. DSM 5940
Status Quality: questionable
The quality level of this entry.

Status: active
The status of this entry.

Completeness: complete
Whether the entry covers everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • NRPS (Type I)
  • other (nucleoside)
Loci
HQ287563.1
via Heterologous expression
Compounds
  • napsamycin A
  • napsamycin B
  • napsamycin C
  • napsamycin D
  • mureidomycin A
  • mureidomycin B
Species Streptomyces sp. DSM 5940 [taxonomy]
References
Chemical products information
napsamycin A Evidence:
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C39H48N8O12S1
Chemical database entries
PubCHEM
napsamycin B Evidence:
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C40H50N8O12S1
Chemical database entries
PubCHEM
napsamycin C Evidence:
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C39H50N8O12S1
Chemical database entries
NPAtlas
napsamycin D Evidence:
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C40H52N8O12S1
Chemical database entries
NPAtlas
mureidomycin A Evidence:
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C38H50N8O12S1
Chemical database entries
PubCHEM
mureidomycin B Evidence:
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C38H50N8O12S1
Chemical database entries
PubCHEM
PubCHEM
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • ADY76657.1
  • npsA
 1 - 1035 (-) transcriptional regulator
  • Regulation
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  • ADY76658.1
  • npsB
 1184 - 1714 (-) N-acetyltransferase
  • Tailoring
  • Knock-out
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  • ADY76659.1
  • npsC
 1889 - 2656 (+) phosphatase
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    • ADY76660.1
    • npsP1
    		 2783 - 3067 (+) non-ribosomal peptide synthetase
    • Scaffold biosynthesis
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    • ADY76661.1
    • npsP2
    		 3115 - 4338 (+) non-ribosomal peptide synthetase
    • Scaffold biosynthesis
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    • ADY76662.1
    • npsD
    		 4347 - 4571 (+) MbtH-like protein
    • Scaffold biosynthesis
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    • ADY76663.1
    • npsE
    		 4568 - 5899 (+) FAD-depending oxidoreductase
    • Precursor biosynthesis
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    • ADY76664.1
    • npsP3
    		 5930 - 8485 (-) non-ribosomal peptide synthetase
    • Scaffold biosynthesis
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    • ADY76665.1
    • npsF
    		 8505 - 8870 (-) cupin 2 domain-containing protein
    • Precursor biosynthesis
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    • ADY76666.1
    • npsP4
    		 8874 - 10535 (-) non-ribosomal peptide synthetase
    • Scaffold biosynthesis
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    • ADY76667.1
    • npsP5
    		 10789 - 13350 (+) non-ribosomal peptide synthetase
    • Scaffold biosynthesis
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    • ADY76668.1
    • npsG
    		 13605 - 14870 (+) argininosuccinate lyase
    • Precursor biosynthesis
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    • ADY76669.1
    • npsH
    		 14867 - 15790 (+) homoserine kinase
    • Precursor biosynthesis
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    • ADY76670.1
    • npsI
    		 15808 - 18075 (+) DABA synthase
    • Precursor biosynthesis
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    • ADY76671.1
    • npsJ
    		 18092 - 19135 (+) threonine aldolase
    • Precursor biosynthesis
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    • ADY76672.1
    • npsK
    		 19263 - 20105 (-) phenylalanine 3-hydroxylase
    • Precursor biosynthesis
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    • ADY76673.1
    • npsL
    		 20112 - 20600 (-) hypothetical protein
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      • ADY76674.1
      • npsP6
      		 20727 - 22250 (-) non-ribosomal peptide synthetase
      • Scaffold biosynthesis
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      • ADY76675.1
      • npsM
      		 22944 - 23765 (+) hypothetical protein
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        • ADY76676.1
        • npsN
        		 23760 - 24812 (-) tRNA-dependent amide synthetase
        • Scaffold biosynthesis
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        • ADY76677.1
        • npsO
        		 24865 - 26148 (-) exporter
        • Transport
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        • ADY76678.1
        • npsP7
        		 26411 - 27607 (+) non-ribosomal peptide synthetase
        • Scaffold biosynthesis
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        • ADY76679.1
        • npsQ
        		 27635 - 28912 (+) aminotransferase
        • Precursor biosynthesis
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        • ADY76680.1
        • npsR
        		 28937 - 29872 (+) dioxygenase
        • Precursor biosynthesis
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        • ADY76681.1
        • npsS
        		 29994 - 30728 (+) hypothetical protein
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          • ADY76682.1
          • npsT
          		 30800 - 31138 (+) hypothetical protein
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            • ADY76683.1
            • npsU
            		 31135 - 32118 (+) FMN-depending oxidoreductase
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              • ADY76684.1
              • npsP8
              		 32220 - 33788 (-) non-ribosomal peptide synthetase
              • Scaffold biosynthesis
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              • ADY76685.1
              • npsV
              		 33811 - 34581 (-) methyltransferase
              • Tailoring
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              Biosynthesis information

              Biosynthetic modules

              Name
              Unk03
              Type
              nrps-type1
              Genes
              npsP3
              Substrates
              meta-tyrosine (evidence: Sequence-based prediction)
              Integrated Monomers
              Domains
              adenylation
              Name
              Unk05
              Type
              nrps-type1
              Genes
              npsP5
              Substrates
              2S,3S-diaminobutyric acid (evidence: Sequence-based prediction)
              Integrated Monomers
              Domains
              adenylation
              Name
              Unk06
              Type
              nrps-type1
              Genes
              npsP6
              Substrates
              meta-tyrosine (evidence: Sequence-based prediction)
              Integrated Monomers
              Domains
              adenylation
              Name
              Unk08
              Type
              nrps-type1
              Genes
              npsP8
              Substrates
              methionine (evidence: Sequence-based prediction)
              Integrated Monomers
              Domains
              adenylation
              Annotation changelog

              Entry version: 4

              Date
              Changes
              Submitters
              Reviewers
              2022-10-07
              Update chemical activity to schema version 2.11
              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

              Entry version: 3

              Date
              Changes
              Submitters
              Reviewers
              2022-09-15
              Corrected NRP module activity
              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
              2022-09-15
              Updated bioactivity data
              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
              2022-09-15
              Updated NRP substrate specificities
              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

              Entry version: 2

              Date
              Changes
              Submitters
              Reviewers
              2019-10-16
              Migrated from v1.4
              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
              2019-10-16
              Updated compound(s) information (NPAtlas curation)
              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

              Entry version: 1

              Date
              Changes
              Submitters
              Reviewers
              2015-06-12
              Submitted
              • (ID: MQCAQSV425YQGLSERITRLP7K)
              • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
              Detailed domain annotation
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