BGC0000963: bleomycin biosynthetic gene cluster from Streptomyces verticillus
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Location: 1 - 77,457 nt. (total: 77,457 nt).
This entry is originally from NCBI GenBank AF210249.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
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resistance
TTA codons
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Gene details
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General
Compounds
Genes
Polyketide
NRP
Saccharide
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0000963
Short description bleomycin biosynthetic gene cluster from Streptomyces verticillus
Status Minimal annotation: no
A minimal annotation only contains information on the BGC loci and one or more linked chemical product(s)

Completeness: complete
Whether the loci encodes everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • NRP (Glycopeptide)
  • Polyketide (Other)
  • Saccharide (hybrid/tailoring)
Loci NCBI GenBank: AF210249.1
Compounds
  • bleomycin
Species Streptomyces verticillus [taxonomy]
References
Chemical products information
bleomycin
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C55H84N17O21S3
Chemical database entries
PubCHEM
NPAtlas
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • AAG02342.1
 1199 - 2185 (-) SyrP-like protein
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  • AAG02343.1
 2182 - 3918 (-) peptide synthetase NRPS12
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  • AAG02344.1
 3915 - 4733 (-) hypothetical protein
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  • AAG02345.1
 4741 - 6024 (-) putative transporter
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  • AAG02346.1
 6021 - 6773 (-) thioesterase
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  • AAG02347.1
 6784 - 7797 (-) clavaminic acid synthase-like protein
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  • AAG02348.1
 7859 - 9214 (-) putative transporter
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  • AAG02349.1
 9211 - 17736 (-) peptide synthetase NRPS11-10
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  • AAG02350.1
 18307 - 18660 (-) PHNA-like protein
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  • AAG02351.1
 18823 - 19164 (+) metal-dependent regulatory protein
  • Regulation
Sequence-based prediction
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  • AAG02352.1
 19161 - 20909 (+) putative transporter
  • Transport
Sequence-based prediction
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  • AAG02353.1
 21367 - 22086 (+) phosphatidylserine decarboxylase
  • Unknown
Sequence-based prediction
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  • AAG02354.1
  • blmXI
 22127 - 24193 (+) peptide synthetase NRPS
  • Unknown
Sequence-based prediction
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  • AAG02355.1
  • blmX
 24406 - 30894 (+) peptide synthetase NRPS9-8
  • Scaffold biosynthesis
Sequence-based prediction
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  • AAG02356.1
  • blmIX
 30891 - 34091 (+) peptide synthetase NRPS7
  • Scaffold biosynthesis
Sequence-based prediction
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  • AAG02357.1
  • blmVIII
 34088 - 39613 (+) polyketide synthase
  • Scaffold biosynthesis
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  • AAG02358.1
  • blmVII
 39610 - 43266 (+) peptide synthetase NRPS6
  • Scaffold biosynthesis
Sequence-based prediction
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  • AAG02359.1
  • blmVI
 43263 - 51290 (+) peptide synthetase NRPS5-4-3
  • Scaffold biosynthesis
Sequence-based prediction
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  • AAG02360.1
  • blmV
 51493 - 53430 (+) peptide synthetase NRPS3CT
  • Scaffold biosynthesis
Sequence-based prediction
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  • AAG02361.1
  • blmG
 53427 - 54404 (+) sugar epimerase BlmG
  • Tailoring (Epimerization)
Sequence-based prediction
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  • AAG02362.1
  • blmF
 54418 - 55902 (+) hydroxylase BlmF
  • Tailoring (Unknown)
Sequence-based prediction
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  • AAG02363.1
 55899 - 57815 (+) asparagine synthetase
  • Precursor biosynthesis
Sequence-based prediction
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  • AAG02364.1
  • blmIV
 57901 - 65781 (+) peptide synthetase NRPS2-1
  • Scaffold biosynthesis
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  • AAG02365.1
  • blmIII
 65778 - 68585 (+) peptide synthetase NRPS0
  • Scaffold biosynthesis
Activity assay
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  • AAG02366.1
 68582 - 69601 (+) SyrP-like protein
  • Regulation
Sequence-based prediction
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  • AAG02367.1
  • blmII
 69598 - 70986 (+) peptide synthetase NRPS
  • Unknown
Sequence-based prediction
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  • AAG02368.1
 70983 - 71546 (+) MbtH-like protein
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  • AAG02369.1
  • blmE
 71618 - 72790 (+) sugar transferase BlmE
  • Tailoring (Glycosylation)
Sequence-based prediction
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  • AAG02370.1
  • blmD
 72787 - 74424 (+) carbamyltransferase BlmD
  • Tailoring (Acylation)
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  • AAD42077.1
  • blmI
 74421 - 74693 (+) peptide synthetase NRPS type II-PCP
  • Scaffold biosynthesis
Activity assay
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  • AAG02371.1
  • blmC
 74690 - 76186 (+) sugar synthase BlmC
  • Activation / processing
Sequence-based prediction
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  • AAG02372.1
 76183 - 77457 (+) coproporphyrinogen III oxidase-like protein
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Polyketide-specific information
Subclass Other
Cyclic? no
Polyketide-synthases
Genes Properties Modules
blmVIII Synthase subclass: Modular type I
Module 7
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: blmVIII
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
Scaffold-modifying domains: Methylation
KR-domain stereochemistry: D-OH
NRP-specific information
Subclass Glycopeptide
Cyclic? no
NRP-synthases
Gene Modules
blmX
Module 3
Specificity: asparagine
Evidence for specificity:
  • Sequence-based prediction
Condensation domain type: LCL
Module 4
Specificity: histidine
Evidence for specificity:
  • Sequence-based prediction
Condensation domain type: LCL
blmIX
Module 5
Specificity: alanine
Evidence for specificity:
  • Sequence-based prediction
Condensation domain type: LCL
blmVII
Module 6
Specificity: threonine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
blmVI
Module 0
Condensation domain type: Starter
Module 1
Specificity: serine
Evidence for specificity:
  • Sequence-based prediction
Condensation domain type: LCL
Module 2
Specificity: asparagine
Evidence for specificity:
  • Sequence-based prediction
Condensation domain type: LCL
blmV
Module 2
Trans-activates asparagine

Condensation domain type: LCL
blmIV
Module 7
Specificity: beta-alanine
Evidence for specificity:
  • Sequence-based prediction
Condensation domain type: LCL
Module 8
Trans-activates BlmIII

Specificity: cysteine
Evidence for specificity:
  • Sequence-based prediction
Condensation domain type: Heterocyclization
blmIII
Module 9 [inactive]
Trans-activates cysteine

Modification domains: Oxidation
Saccharide-specific information
Subclass hybrid/tailoring
Annotation changelog
MIBiG version Submitter Notes
1.0
  • Hidden contributor (ID: E2T2HCM4X7QXVS4LL4JVBPPK, no GDPR consent given).
  • Submitted
2.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Migrated from v1.4
3.0
  • Hidden contributor (ID: H5KVDLSVQ3TJW7SKCDNBDEVC, no GDPR consent given).
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Linked new NPAtlas id for bleomycin (npatlas:NPA020036)
  • Corrected NRP module activity
  • Removed substrate from blmV gene
  • Fixed module numbers
  • Updated NRP substrate specificities
3.1
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Update chemical activity to schema version 2.11
Detailed domain annotation
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