BGC0000967: calyculin A biosynthetic gene cluster from uncultured Candidatus Entotheonella sp.
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Location: 1 - 148,229 nt. (total: 148,229 nt).
This entry is originally from NCBI GenBank AB933566.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
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Gene details
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General
Compounds
Genes
Biosynthesis
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0000967
Short description calyculin A biosynthetic gene cluster from uncultured Candidatus Entotheonella sp.
Status Quality: questionable
The quality level of this entry.

Status: active
The status of this entry.

Completeness: complete
Whether the entry covers everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • NRPS (Type I)
  • PKS (Trans-AT type I)
Loci
AB933566.1
via Enzymatic assays
Compounds
  • calyculin A
  • phosphocalyculin A
  • calyculinamide A
  • dephosphonocalyculin A
Species uncultured Candidatus Entotheonella sp. [taxonomy]
References
Chemical products information
calyculin A Evidence:
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C50H81N4O15P1
Chemical database entries
PubCHEM
phosphocalyculin A Evidence:
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C50H82N4O18P2
calyculinamide A Evidence:
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C50H83N4O16P1
Chemical database entries
PubCHEM
ChemSpider
dephosphonocalyculin A Evidence:
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C50H80N4O12
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • BAP05573.1
  • calY
 1 - 2364 (+) calY
  • Scaffold biosynthesis
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  • BAP05574.1
  • calX
 2395 - 2643 (+) calX
  • Scaffold biosynthesis
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  • BAP05575.1
  • calW
 2621 - 3850 (+) calW
  • Scaffold biosynthesis
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  • BAP05576.1
  • calV
 4030 - 4428 (+) calV
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    • BAP05577.1
    • calU
    		 4425 - 6545 (+) calU
    • Transport
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    • BAP05578.1
    • calT
    		 6647 - 7921 (+) calT
    • Scaffold biosynthesis
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    • BAP05579.1
    • calS
    		 7909 - 8715 (+) calS
    • Scaffold biosynthesis
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    • BAP05580.1
    • calR
    		 8708 - 9445 (+) calR
    • Scaffold biosynthesis
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    • BAP05581.1
    • calQ
    		 9473 - 10606 (+) calQ
    • Tailoring
    • Activity assay
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    • BAP05582.1
    • calP
    		 10603 - 11298 (+) calP
    • Tailoring
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    • BAP05583.1
    • calO
    		 11313 - 12122 (-) calO
    • Resistance/immunity
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    • BAP05584.1
    • calN
    		 12379 - 13650 (-) calN
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      • BAP05585.1
      • calM
      		 14351 - 15049 (+) calM
      • Tailoring
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      • BAP05586.1
      • calL
      		 15257 - 16411 (+) calL
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        • BAP05587.1
        • calK
        		 16776 - 17969 (+) calK
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          • BAP05588.1
          • calJ
          		 18377 - 20068 (+) calJ
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            • BAP05589.1
            • calA
            		 20404 - 31761 (+) calA
            • Scaffold biosynthesis
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            • BAP05590.1
            • calB
            		 31828 - 51879 (+) calB
            • Scaffold biosynthesis
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            • BAP05591.1
            • calC
            		 51876 - 65756 (+) calC
            • Scaffold biosynthesis
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            • BAP05592.1
            • calD
            		 65768 - 66895 (+) calD
            • Tailoring
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            • BAP05593.1
            • calE
            		 66955 - 90753 (+) calE
            • Scaffold biosynthesis
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            • BAP05594.1
            • calF
            		 90764 - 108340 (+) calF
            • Scaffold biosynthesis
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            • BAP05595.1
            • calG
            		 108337 - 125763 (+) calG
            • Scaffold biosynthesis
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            • BAP05596.1
            • calH
            		 125766 - 140192 (+) calH
            • Scaffold biosynthesis
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            • BAP05597.1
            • calI
            		 140205 - 148229 (+) calI
            • Scaffold biosynthesis
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            Biosynthesis information

            Biosynthetic modules

            Name
            0
            Type
            nrps-type1
            Genes
            calA
            Substrates
            serine (evidence: Sequence-based prediction)
            Integrated Monomers
            Domains
            adenylation, methyltransferase
            Name
            4
            Type
            nrps-type1
            Genes
            calB
            Substrates
            glycine (evidence: Sequence-based prediction)
            Integrated Monomers
            Domains
            condensation (LCL), adenylation
            Name
            8
            Type
            nrps-type1
            Genes
            calC
            Substrates
            serine (evidence: Sequence-based prediction)
            Integrated Monomers
            Domains
            adenylation
            Name
            29
            Type
            nrps-type1
            Genes
            calH
            Substrates
            alanine (evidence: Sequence-based prediction)
            Integrated Monomers
            Domains
            condensation (LCL), adenylation
            Name
            33
            Type
            nrps-type1
            Genes
            calI
            Substrates
            serine (evidence: Sequence-based prediction)
            Integrated Monomers
            Domains
            condensation (LCL), adenylation
            Name
            1
            Type
            pks-trans-at
            Genes
            calA
            Substrates
            Integrated Monomers
            Domains
            ketosynthase, methyltransferase, carrier (ACP)
            Name
            2
            Type
            pks-trans-at
            Genes
            calA
            Substrates
            Integrated Monomers
            Domains
            ketosynthase, methyltransferase, carrier (ACP)
            Name
            3
            Type
            pks-trans-at
            Genes
            calB
            Substrates
            Integrated Monomers
            Domains
            ketosynthase, carrier (ACP)
            Name
            5
            Type
            pks-trans-at
            Genes
            calB
            Substrates
            Integrated Monomers
            Domains
            ketosynthase, dehydratase, ketoreductase, enoylreductase, carrier (ACP)
            Name
            6
            Type
            pks-trans-at
            Genes
            calB
            Substrates
            Integrated Monomers
            Domains
            ketosynthase, carrier (ACP)
            Name
            7
            Type
            pks-trans-at
            Genes
            calB
            Substrates
            Integrated Monomers
            Domains
            ketosynthase, carrier (ACP)
            Name
            9
            Type
            pks-trans-at
            Genes
            calC
            Substrates
            Integrated Monomers
            Domains
            ketosynthase, oxidase, carrier (ACP)
            Name
            10
            Type
            pks-trans-at
            Genes
            calC
            Substrates
            Integrated Monomers
            Domains
            ketosynthase, dehydratase, ketoreductase, oxidase, carrier (ACP)
            Name
            11
            Type
            pks-trans-at
            Genes
            calC
            Substrates
            Integrated Monomers
            Domains
            ketosynthase, ketoreductase, oxidase, carrier (ACP)
            Name
            12
            Type
            pks-trans-at
            Genes
            calE
            Substrates
            Integrated Monomers
            Domains
            ketosynthase, dehydratase, carrier (ACP)
            Name
            13
            Type
            pks-trans-at
            Genes
            calE
            Substrates
            Integrated Monomers
            Domains
            ketosynthase
            Name
            14
            Type
            pks-trans-at
            Genes
            calE
            Substrates
            Integrated Monomers
            Domains
            ketosynthase, ketoreductase, carrier (ACP)
            Name
            15
            Type
            pks-trans-at
            Genes
            calE
            Substrates
            Integrated Monomers
            Domains
            ketosynthase, ketoreductase, carrier (ACP)
            Name
            16
            Type
            pks-trans-at
            Genes
            calE
            Substrates
            Integrated Monomers
            Domains
            ketosynthase
            Name
            17
            Type
            pks-trans-at
            Genes
            calE
            Substrates
            Integrated Monomers
            Domains
            ketosynthase, carrier (ACP)
            Name
            18
            Type
            pks-trans-at
            Genes
            calE
            Substrates
            Integrated Monomers
            Domains
            ketosynthase, ketoreductase
            Name
            19
            Type
            pks-trans-at
            Genes
            calF
            Substrates
            Integrated Monomers
            Domains
            ketosynthase, carrier (ACP)
            Name
            20
            Type
            pks-trans-at
            Genes
            calF
            Substrates
            Integrated Monomers
            Domains
            ketosynthase, ketoreductase, carrier (ACP)
            Name
            21
            Type
            pks-trans-at
            Genes
            calF
            Substrates
            Integrated Monomers
            Domains
            ketosynthase, carrier (ACP)
            Name
            22
            Type
            pks-trans-at
            Genes
            calF
            Substrates
            Integrated Monomers
            Domains
            ketosynthase, ketoreductase, carrier (ACP)
            Name
            23
            Type
            pks-trans-at
            Genes
            calF calG
            Substrates
            Integrated Monomers
            Domains
            ketosynthase, ketoreductase, methyltransferase, carrier (ACP)
            Name
            24
            Type
            pks-trans-at
            Genes
            calG
            Substrates
            Integrated Monomers
            Domains
            ketosynthase, dehydratase, ketoreductase, methyltransferase, carrier (ACP)
            Name
            25
            Type
            pks-trans-at
            Genes
            calG
            Substrates
            Integrated Monomers
            Domains
            ketosynthase, methyltransferase
            Name
            26
            Type
            pks-trans-at
            Genes
            calG
            Substrates
            Integrated Monomers
            Domains
            ketosynthase, dehydratase, ketoreductase, methyltransferase, carrier (ACP)
            Name
            27
            Type
            pks-trans-at
            Genes
            calG
            Substrates
            Integrated Monomers
            Domains
            ketosynthase, methyltransferase
            Name
            28
            Type
            pks-trans-at
            Genes
            calH
            Substrates
            Integrated Monomers
            Domains
            ketosynthase
            Name
            30
            Type
            pks-trans-at
            Genes
            calH
            Substrates
            Integrated Monomers
            Domains
            ketosynthase, dehydratase, ketoreductase
            Name
            31
            Type
            pks-trans-at
            Genes
            calH calI
            Substrates
            Integrated Monomers
            Domains
            ketosynthase, dehydratase, carrier (ACP)
            Name
            32
            Type
            pks-trans-at
            Genes
            calI
            Substrates
            Integrated Monomers
            Domains
            ketosynthase
            Annotation changelog

            Entry version: 4

            Date
            Changes
            Submitters
            Reviewers
            2022-10-07
            Update chemical activity to schema version 2.11
            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

            Entry version: 3

            Date
            Changes
            Submitters
            Reviewers
            2022-09-15
            Corrected NRP module activity
            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
            2022-09-15
            Removed ketoreductase stereochemistry annotation from modules without ketoreductases
            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
            2022-09-15
            Merged cross-CDS PKS modules
            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
            2022-09-15
            Changed amino acid substrates to lower case
            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

            Entry version: 2

            Date
            Changes
            Submitters
            Reviewers
            2019-10-16
            Migrated from v1.4
            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
            2019-10-16
            Updated compound(s) information (NPAtlas curation)
            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

            Entry version: 1

            Date
            Changes
            Submitters
            Reviewers
            2015-06-12
            Submitted
            • (ID: CUIZDUBUBVAF27VHF4OZXTI3)
            • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
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