BGC0001016: microcystin biosynthetic gene cluster from Anabaena sp. 90
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Location: 1 - 57,817 nt. (total: 57,817 nt).
This entry is originally from NCBI GenBank AY212249.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
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Gene details
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General
Compounds
Genes
Polyketide
NRP
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0001016
Short description microcystin biosynthetic gene cluster from Anabaena sp. 90
Status Minimal annotation: no
A minimal annotation only contains information on the BGC loci and one or more linked chemical product(s)

Completeness: complete
Whether the loci encodes everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • NRP
  • Polyketide
Loci NCBI GenBank: AY212249.1
Compounds
  • microcystin
Species Anabaena sp. 90 [taxonomy]
References
Chemical products information
microcystin
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C49H74N10O12
Chemical database entries
PubCHEM
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • AAO62577.1
 174 - 566 (+) hypothetical protein
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  • AAO62578.1
 972 - 1328 (+) hypothetical protein
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  • AAO62579.1
  • mcyH
 1301 - 3079 (-) ABC transporter ATP-binding-like protein
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  • AAO62580.1
  • mcyI
 3374 - 4387 (-) phosphoglycerate dehydrogenase-like protein
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  • AAO62581.1
  • mcyF
 4438 - 5196 (-) amino acid racemase
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  • AAO62582.1
  • mcyE
 5238 - 15686 (-) polyketide synthase peptide sythetase fusion protein
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  • AAO62583.1
  • mcyJ
 15723 - 16655 (-) putative O-methyl transferase
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  • AAO62584.1
  • mcyD
 16714 - 28323 (-) polyketide synthase type 1
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  • AAO62585.1
  • mcyG
 28417 - 36324 (-) peptide sythetase polyketide synthase fusion protein
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  • AAO62586.1
  • mcyA
 37522 - 45888 (+) peptide sythetase
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  • AAO62587.1
  • mcyB
 45906 - 52307 (+) peptide sythetase
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  • AAO62588.1
  • mcyC
 52304 - 56158 (+) peptide sythetase
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  • AAO62589.1
  • atn1
 56675 - 57817 (+) putative transposase
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Polyketide-specific information
Subclass Unknown
Cyclic? no
Polyketide-synthases
Genes Properties Modules
mcyD
+
mcyG
+
mcyE 		Synthase subclass:
Module 1
Specificity: Malonyl-CoA
Genes: mcyG
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
Scaffold-modifying domains: Methylation
KR-domain stereochemistry: Unknown
Module 2
Genes: mcyD
Core domains: Ketosynthase, Dehydratase, Ketoreductase, Thiolation (ACP/PCP)
Scaffold-modifying domains: Methylation
KR-domain stereochemistry: Unknown
Module 3
Specificity: Malonyl-CoA
Genes: mcyD
Core domains: Ketosynthase, Acyltransferase, Dehydratase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: Unknown
Module 4
Specificity: Malonyl-CoA
Genes: mcyE
Core domains: Ketosynthase, Acyltransferase, Thiolation (ACP/PCP)
Scaffold-modifying domains: Methylation
NRP-specific information
Subclass N/A
Cyclic? yes
Release type
  • Hydrolysis
NRP-synthases
Gene Modules
mcyC
Module 11
Specificity: arginine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
mcyB
Module 9
Specificity: leucine / arginine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: DCL
Module 10
Specificity: 3S-methyl-D-aspartic acid branched / aspartic acid branched
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
mcyA
Module 7
Specificity: serine
Evidence for specificity:
  • Structure-based inference
  • Homology
Modification domains: Methylation
Module 8
Specificity: alanine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: DCL
mcyG
Module 0
Specificity: cinnamic acid / hydrocinnamic acid / D-phenylalanine
Evidence for specificity:
  • Structure-based inference
  • Homology
mcyE
Module 5
Condensation domain type: LCL
Modification domains: Aminotransferase
Module 6
Specificity: D-glutamic acid branched
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
Annotation changelog
MIBiG version Submitter Notes
1.0
  • Hidden contributor (ID: BAJIZJS2YANDM4M7JHS5DFUK, no GDPR consent given).
  • Submitted
2.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Migrated from v1.4
3.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Corrected NRP module activity
  • Removed ketoreductase stereochemistry annotation from modules without ketoreductases
  • Updated NRP substrate specificities
3.1
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Update chemical activity to schema version 2.11
Detailed domain annotation
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