BGC0001017: microcystin LR biosynthetic gene cluster from Microcystis aeruginosa PCC 7806
Shows the layout of the region, marking coding sequences and areas of interest. Clicking a gene will select it and show any relevant details. Clicking an area feature (e.g. a candidate cluster) will select all coding sequences within that area. Double clicking an area feature will zoom to that area. Multiple genes and area features can be selected by clicking them while holding the Ctrl key.
View antiSMASH-generated output
Download Cluster GenBank file
Download region SVG
Location: 1 - 63,626 nt. (total: 63,626 nt).
This entry is originally from NCBI GenBank AF183408.1.

Legend:

core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
reset zoomreset view
zoomzoom to selection
Gene details
Shows details of the most recently selected gene, including names, products, location, and other annotations.
Select a gene to view the details available for it
General
Compounds
Genes
Polyketide
NRP
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0001017
Short description microcystin LR biosynthetic gene cluster from Microcystis aeruginosa PCC 7806
Status Minimal annotation: no
A minimal annotation only contains information on the BGC loci and one or more linked chemical product(s)

Completeness: complete
Whether the loci encodes everything needed for the pathway producing the compound(s)
Remarks "This cyclic peptide is an inhibitor of eukaryotic protein phosphatases type 1 and 2A. Synthesized nonribosomally by NRPS, PKS and hybrid PKS-NRPS enzymes, the microcystins contain a number of unusual amino acid residues, including the L-amino polyketide moiety Adda (3-amino-9-methoxy-2,6,8-trimethyl-10-phenyl-4,6-decadienoic acid)."
Biosynthetic class(es)
  • NRP
  • Polyketide (Other)
Loci NCBI GenBank: AF183408.1
Compounds
  • microcystin LR
Species Microcystis aeruginosa PCC 7806 [taxonomy]
References
Chemical products information
microcystin LR [synonyms: cyanoginosin lr, toxin i cyanobacterium]
Copy SMILES
C49H74N10O12
Chemical database entries
PubCHEM
ChEBI
ChEMBL
ChemSpider
NPAtlas
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • AAF00953.1
  • dnaN
 3 - 422 (+) DNA polymerase III beta subunit
copy AA seq
copy Nt seq
  • AAF00954.1
  • mcyJ
 992 - 1828 (-) mcyJ
  • Tailoring (Methylation)
Knock-out
copy AA seq
copy Nt seq
  • AAF00955.1
  • mcyI
 2004 - 3017 (-) mcyI
  • Tailoring (Reduction)
Activity assay
copy AA seq
copy Nt seq
  • AAF00956.1
  • mcyH
 3056 - 4672 (-) mcyH
  • Transport
Knock-out
copy AA seq
copy Nt seq
  • AAF00957.1
  • mcyG
 4896 - 12791 (-) mcyG
copy AA seq
copy Nt seq
  • AAF00963.1
  • mcyF
 12923 - 13678 (-) mcyF
copy AA seq
copy Nt seq
  • AAF00958.1
  • mcyE
 13710 - 24173 (-) mcyE
copy AA seq
copy Nt seq
  • AAF00959.1
  • mcyD
 24340 - 36060 (-) mcyD
  • Scaffold biosynthesis
Knock-out
copy AA seq
copy Nt seq
  • AAF00960.1
  • mcyA
 36793 - 45180 (+) mcyA
  • Scaffold biosynthesis
Knock-out
copy AA seq
copy Nt seq
  • AAF00961.1
  • mcyB
 45196 - 51576 (+) mcyB
  • Scaffold biosynthesis
Knock-out
copy AA seq
copy Nt seq
  • AAF00962.1
  • mcyC
 51573 - 55448 (+) mcyC
copy AA seq
copy Nt seq
  • AAF00964.1
  • uma1
 55541 - 57592 (-) uma1
copy AA seq
copy Nt seq
  • AAF00965.1
  • uma2
 57836 - 58399 (-) uma2
copy AA seq
copy Nt seq
  • AAF00966.1
  • uma3
 58446 - 59972 (-) uma3
copy AA seq
copy Nt seq
  • AAF00967.1
  • uma4
 60057 - 61271 (-) uma4
copy AA seq
copy Nt seq
  • AAF00968.1
  • uma5
 62180 - 62614 (-) uma5
copy AA seq
copy Nt seq
  • AAF00969.1
  • uma6
 62656 - >63624 (-) uma6
copy AA seq
copy Nt seq
Polyketide-specific information
Subclass Other
Cyclic? yes
Release type
  • Hydrolysis
Polyketide-synthases
Genes Properties Modules
mcyD
+
mcyE
+
mcyG 		Synthase subclass: Modular type I
Module 1
Genes: mcyG
Core domains: Ketosynthase, Acyltransferase, Ketoreductase, Thiolation (ACP/PCP)
Scaffold-modifying domains: Methylation
KR-domain stereochemistry: Unknown
Module 2
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: mcyE
Core domains: Ketosynthase, Acyltransferase
Scaffold-modifying domains: Methylation
Module 5
Genes: mcyD
Core domains: Ketosynthase, Dehydratase, Ketoreductase, Thiolation (ACP/PCP)
Scaffold-modifying domains: Methylation
KR-domain stereochemistry: Unknown
Module 6
Specificity: Malonyl-CoA
Evidence for specificity: Structure-based inference
Genes: mcyD
Core domains: Ketosynthase, Acyltransferase, Dehydratase, Ketoreductase, Thiolation (ACP/PCP)
KR-domain stereochemistry: Unknown
NRP-specific information
Subclass N/A
Cyclic? yes
Release type
  • Hydrolysis
Thioesterase genes
  • mcyC (Type I)
NRP-synthases
Gene Modules
mcyG
Module 0
Specificity: cinnamic acid
Evidence for specificity:
  • Structure-based inference
  • Homology
mcyE
Module 3
Specificity: D-glutamic acid branched
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
Module 4
Condensation domain type: LCL
mcyA
Module 6
Specificity: serine
Evidence for specificity:
  • Structure-based inference
Modification domains: Methylation
Module 7
Specificity: alanine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: DCL
mcyB
Module 8
Specificity: leucine / valine / tyrosine / isoleucine / glycine
Evidence for specificity:
  • ATP-PPi exchange assay
Condensation domain type: DCL
Module 9
Specificity: 3S-methyl-D-aspartic acid branched / D-aspartic acid branched
Evidence for specificity:
  • Structure-based inference
  • Feeding study
Condensation domain type: LCL
mcyC
Module 10
Specificity: valine / leucine / isoleucine / arginine / glycine / lysine
Evidence for specificity:
  • ATP-PPi exchange assay
Condensation domain type: LCL
Annotation changelog
MIBiG version Submitter Notes
1.0
  • Hidden contributor (ID: EI7LXM427LE4WIOQ5U54V3BB, no GDPR consent given).
  • Submitted
2.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Migrated from v1.4
3.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Linked new NPAtlas id for microcystin LR (npatlas:NPA0593)
  • Corrected NRP module activity
  • Removed ketoreductase stereochemistry annotation from modules without ketoreductases
  • Updated NRP substrate specificities
3.1
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Update chemical activity to schema version 2.11
Detailed domain annotation
Shows NRPS- and PKS-related domains for each feature that contains them. Click on each domain for more information about the domain's location, consensus monomer prediction, and other details.
A glossary is available here.
Selected features only
Show module domains
Similar known gene clusters
Shows clusters from the MiBIG database that are similar to the current region. Genes marked with the same colour are interrelated. White genes have no relationship.
Click on reference genes to show details of similarities to genes within the current region.
Click on an accession to open that entry in the MiBIG database.