BGC0001470: macrobrevin biosynthetic gene cluster from Brevibacillus sp. Leaf182
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Location: 223,174 - 318,348 nt. (total: 95,175 nt).
This entry is originally from NCBI GenBank LMPN02000017.1.

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core biosynthetic genes
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transport-related genes
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Gene details
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General
Compounds
Genes
Biosynthesis
Operons
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0001470
Short description macrobrevin biosynthetic gene cluster from Brevibacillus sp. Leaf182
Status Quality: questionable
The quality level of this entry.

Status: active
The status of this entry.

Completeness: complete
Whether the entry covers everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • PKS (Trans-AT type I)
Loci
LMPN02000017.1
223174 - 318348
via Knock-out studies
Compounds
  • macrobrevin
Species Brevibacillus sp. Leaf182 [taxonomy]
References
Chemical products information
macrobrevin Evidence: NMR, Mass spectrometry
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C42H66O6
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • ASG16_007415
  • RAT98517.1
  • breO
 223174 - 237495 (-) trans-acyltransferase polyketide synthase
  • Scaffold biosynthesis
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  • ASG16_007420
  • RAT98518.1
  • breN
 237539 - 247891 (-) trans-acyltransferase polyketide synthase
  • Scaffold biosynthesis
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  • ASG16_007425
  • RAT98519.1
  • breM
 247930 - 253734 (-) trans-acyltransferase polyketide synthase
  • Scaffold biosynthesis
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  • ASG16_007430
  • RAT98520.1
  • breL
 253757 - 254503 (-) enoyl-CoA hydratase
  • Scaffold biosynthesis
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  • ASG16_007435
  • RAT98521.1
  • breK
 254510 - 255274 (-) enoyl-CoA hydratase
  • Scaffold biosynthesis
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  • ASG16_007440
  • RAT98522.1
  • breJ
 255274 - 256524 (-) 3-hydroxy-3-methylglutaryl-ACP synthase
  • Scaffold biosynthesis
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  • ASG16_007445
  • RAT98523.1
  • breI
 256549 - 257787 (-) polyketide beta-ketoacyl:ACP synthase
  • Scaffold biosynthesis
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  • ASG16_007450
  • RAT98524.1
  • breH
 257765 - 258007 (-) acyl carrier protein
  • Scaffold biosynthesis
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  • ASG16_007455
  • RAT98525.1
  • breG
 258004 - 271413 (-) trans-acyltransferase polyketide synthase
  • Scaffold biosynthesis
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  • ASG16_007460
  • RAT98526.1
  • breF
 271432 - 276963 (-) trans-acyltransferase polyketide synthase
  • Scaffold biosynthesis
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  • ASG16_007465
  • RAT98527.1
  • breE
 276988 - 292734 (-) trans-acyltransferase polyketide synthase
  • Scaffold biosynthesis
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  • ASG16_007470
  • RAT98528.1
  • breD
 292737 - 300644 (-) trans-acyltransferase polyketide synthase
  • Scaffold biosynthesis
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  • ASG16_007475
  • RAT98529.1
  • breC
 300685 - 310422 (-) trans-acyltransferase polyketide synthase
  • Scaffold biosynthesis
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  • ASG16_007480
  • RAT98530.1
  • breB
 310472 - 315031 (-) trans-acyltransferase polyketide synthase
  • Scaffold biosynthesis
  • Knock-out
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  • ASG16_007485
  • RAT98531.1
  • breA
 315049 - 318348 (-) acyltransferase acyltransferase 2-Nitropropane dioxygenase (NPD)-like domain
  • Scaffold biosynthesis
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Biosynthesis information

Biosynthetic modules

Name
1
Type
pks-trans-at
Genes
breB breC
Substrates
Integrated Monomers
Domains
ketosynthase, dehydratase, ketoreductase, methyltransferase, carrier (ACP)
Name
2
Type
pks-trans-at
Genes
breC
Substrates
Integrated Monomers
Domains
ketosynthase, dehydratase, ketoreductase, carrier (ACP)
Name
3
Type
pks-trans-at
Genes
breC breD
Substrates
Integrated Monomers
Domains
ketosynthase, ketoreductase, methyltransferase, carrier (ACP)
Name
4
Type
pks-trans-at
Genes
breD
Substrates
Integrated Monomers
Domains
ketosynthase, dehydratase, ketoreductase, carrier (ACP)
Name
5
Type
pks-trans-at
Genes
breD breE
Substrates
Integrated Monomers
Domains
ketosynthase, dehydratase, carrier (ACP)
Name
6
Type
pks-trans-at
Genes
breE
Substrates
Integrated Monomers
Domains
ketosynthase, dehydratase, methyltransferase, carrier (ACP)
Name
7
Type
pks-trans-at
Genes
breE
Substrates
Integrated Monomers
Domains
ketosynthase, dehydratase, ketoreductase, enoylreductase, methyltransferase, carrier (ACP)
Name
8
Type
pks-trans-at
Genes
breE
Substrates
Integrated Monomers
Domains
ketosynthase
Name
9
Type
pks-trans-at-starter
Genes
breF
Substrates
Integrated Monomers
Domains
ketoreductase, carrier (ACP)
Name
10
Type
pks-trans-at
Genes
breF breG
Substrates
Integrated Monomers
Domains
ketosynthase, ketoreductase, methyltransferase, carrier (ACP)
Name
11
Type
pks-trans-at
Genes
breG
Substrates
Integrated Monomers
Domains
ketosynthase, dehydratase, ketoreductase, carrier (ACP)
Name
12
Type
pks-trans-at
Genes
breG
Substrates
Integrated Monomers
Domains
ketosynthase, dehydratase, ketoreductase, carrier (ACP)
Name
13
Type
pks-trans-at
Genes
breG
Substrates
Integrated Monomers
Domains
ketosynthase, carrier (ACP), carrier (ACP)
Name
14
Type
pks-trans-at
Genes
breM breN
Substrates
Integrated Monomers
Domains
ketosynthase, dehydratase, ketoreductase, methyltransferase, carrier (ACP)
Name
15
Type
pks-trans-at
Genes
breN
Substrates
Integrated Monomers
Domains
ketosynthase, ketoreductase, carrier (ACP)
Name
16
Type
pks-trans-at
Genes
breN
Substrates
Integrated Monomers
Domains
ketosynthase, ketoreductase, carrier (ACP)
Name
17
Type
pks-trans-at
Genes
breN breO
Substrates
Integrated Monomers
Domains
ketosynthase, dehydratase, carrier (ACP)
Name
18
Type
pks-trans-at
Genes
breO
Substrates
Integrated Monomers
Domains
ketosynthase, ketoreductase, methyltransferase, carrier (ACP)
Name
19
Type
pks-trans-at
Genes
breO
Substrates
Integrated Monomers
Domains
ketosynthase, dehydratase, ketoreductase
Name
20
Type
pks-trans-at
Genes
breO
Substrates
Integrated Monomers
Domains
ketosynthase, thioesterase, carrier (ACP)
Operons
Genes Evidence
breJ, breC, breG, breL, breM, breA, breN, breH, breB, breO, breK, breF, breD, breI, breE
Annotation changelog

Entry version: 4

Date
Changes
Submitters
Reviewers
2022-10-07
Update chemical activity to schema version 2.11
  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

Entry version: 3

Date
Changes
Submitters
Reviewers
2022-09-15
Removed ketoreductase stereochemistry annotation from modules without ketoreductases
  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
2022-09-15
Merged cross-CDS PKS modules
  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
2022-09-15
Corrected publication
  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
2022-09-15
Sorted modules by module number
  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

Entry version: 2

Date
Changes
Submitters
Reviewers
2019-10-16
Migrated from v1.4
  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

Entry version: 1

Date
Changes
Submitters
Reviewers
2018-08-06
Submitted
  • (ID: CUIZDUBUBVAF27VHF4OZXTI3)
  • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
Detailed domain annotation
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